KR102263698B1 - A mixture of Lactobacillus plantarum LRCC5195 and isomaltooligosaccharide having ameliorating or improving atopic dermatitis - Google Patents

Abstract

The present invention relates to Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain and isomaltooligosaccharide complex, and uses thereof. More specifically, a complex prepared by mixing the Lactobacillus plantarum LRCC5195 strain, a novel lactic acid bacterium, and isomaltooligosaccharide, which has the effect of inhibiting inflammatory cytokine expression and nitric oxide (NO) production, which is an inflammatory mediator And it relates to the use of alleviating or improving atopic dermatitis.

Description

Lactobacillus plantarum Lactobacillus plantarum Lactobacillus plantarum LRCC5195 and isomaltooligosaccharide having ameliorating or improving atopic dermatitis having atopic dermatitis alleviation or improvement effect

The present invention relates to Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain and isomaltooligosaccharide complex, and uses thereof. More specifically, a complex prepared by mixing the Lactobacillus plantarum LRCC5195 strain, a novel lactic acid bacterium, and isomaltooligosaccharide, which has the effect of inhibiting inflammatory cytokine expression and nitric oxide (NO) production, which is an inflammatory mediator And it relates to the use of alleviating or improving atopic dermatitis.

Atopic dermatitis (AD) is an inflammatory disease accompanied by pruritus, dry skin, eczema, and the like. Although the exact cause has not yet been identified, it is known that the cause of atopic dermatitis known so far is largely due to genetic factors inherited from parents and immunological causes in addition to environmental causes due to urbanization and industrialization. In the past, atopic dermatitis, commonly called fetal fever, occurred well in infancy and childhood, and recently, it is not cured even after adults, and many people are suffering a lot mentally and physically.

Most of patients with atopic dermatitis are characterized by elevated blood IgE levels, and macrophages activated in this inflammatory response are nitric oxide (NO), TNF-α (tumor necrosis factor-α), IL-1 ( It is characterized by releasing interleukin-1) and IL-6. Specifically, the mechanism of inflammation is that cells express COX-2 by stimulation of LPS contained in bacteria, such as NO and PGE ₂ inflammatory mediators or IL-1, TNF-α, and IL-6. Inflammatory cytokines are secreted (Hong JH., The Korean Society For Eco Early Childhood Education , 7(1): 65-81, 2008). As an inflammatory mediator, NO is a highly reactive biogenic molecule and is known to play a role in blood pressure regulation, neurotransmission, blood coagulation, immune function, vasodilation, and antibacterial action (Bang SH et.al. , Korean J Dermatol). ., 42 (1): 29-36 , 2004; Jung SY et.al., J Korean Orient Med Ophthalmol Otolaryngol Dermatol, 22 (1):. 108-19, 2009).

Common atopic dermatitis symptom relievers currently used include moisturizers, antihistamines that reduce pruritus (itchiness), and topical steroids that have therapeutic effects through anti-inflammatory, vasoconstriction, and immunosuppressive action. Steroid-type drugs such as Glucocorticoid and Cyclospoline can cause diabetes and high blood pressure, and cause serious side effects such as Cushing's syndrome, ophthalmic disease (cataract, glaucoma), kidney and liver toxicity. The need for a new therapeutic agent is emerging.

On the other hand, lactic acid bacteria are widely distributed in the digestive tract, oral cavity, feces, various fermented foods and soils of humans or mammals, and are closely related to human life. Lactobacillus and Bifidobacterium are representative lactic acid bacteria represented by probiotics, and these probiotics exhibit various functional effects such as improvement of intestinal flora, improvement of lactose intolerance, prevention of blood lipids and heart disease, anticancer effect and prevention of hypertension. In addition, research results have been reported that lactic acid bacteria have the ability to reduce the accumulation of active oxygen in the body or to remove active oxygen. Therefore, interest in product development using lactic acid bacteria having such an antioxidant effect is increasing.

Hong JH., The Korean Society For Eco Early Childhood Education, 7(1): 65-81, 2008 Bang SH et.al., Korean J Dermatol., 42(1): 29-36, 2004 Jung SY et.al., J Korean Orient Med Ophthalmol Otolaryngol Dermatol., 22(1): 108-19, 2009

Under the above background, the present inventors have made intensive efforts to develop new natural-derived products for alleviating or improving atopic dermatitis, which have excellent effects in treating atopic dermatitis and have fewer side effects. As a result, new lactic acid bacteria isolated from kimchi, a traditional fermented food, and By confirming that the complex prepared by mixing isomaltooligosaccharide has a synergistic effect of inhibiting inflammatory cytokine expression and nitric oxide (NO) production, which is an inflammatory mediator, the present invention was completed.

It is an object of the present invention to provide a Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P.

Another object of the present invention is to provide a food composition for alleviating or improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide as active ingredients.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide as active ingredients.

Another object of the present invention is to provide a cosmetic composition for improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide as active ingredients.

The object of the present invention is not limited to the object mentioned above. The objects of the present invention will become more apparent from the following description, and will be realized by means and combinations thereof described in the claims.

In order to achieve the above object, the following solutions are provided.

One aspect of the present invention provides a Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P.

In one aspect of the present invention, it provides a strain that inhibits the expression of any one of inflammatory cytokines IL-1β, IL-6 and TNF-α, and inhibits the production of inflammatory mediator NO.

In one aspect of the present invention, it provides a strain having an anti-inflammatory effect.

In one aspect of the present invention, it provides a strain comprising the 16S rDNA base sequence of SEQ ID NO: 1.

Another aspect of the present invention provides a food composition for alleviating or improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

In another aspect of the present invention, the composition contains the dead cells of the Lactobacillus plantarum LRCC5195 strain at ^{a concentration of 10 5} to 10 ⁹ CFU/ml, it provides a food composition for alleviating or improving atopic dermatitis.

In another aspect of the present invention, the composition provides a food composition for alleviating or improving atopic dermatitis, further comprising at least one selected from the group consisting of inulin, galactooligosaccharide, sorbitol and allulose.

In another aspect of the present invention, the composition inhibits the expression of any one of the inflammatory cytokines IL-1β, IL-6 and TNF-α, and inhibits the production of inflammatory mediator NO, atopic dermatitis alleviation or improvement food A composition is provided.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

Another aspect of the present invention provides a cosmetic composition for improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain according to one aspect of the present invention is isolated from kimchi, a traditional fermented food, and has high safety, inflammatory cytokine expression and nitric oxide (NO) production, which is an inflammatory mediator has a destructive effect.

The complex prepared by mixing the dead cells and isomaltooligosaccharide of the Lactobacillus plantarum LRCC5195 strain according to an aspect of the present invention inhibits inflammatory cytokine expression and nitric oxide production, which is an inflammatory mediator, compared to the strain or isomaltooligosaccharide alone It has a synergistic effect.

The Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide complex according to an aspect of the present invention significantly inhibits inflammatory cytokine expression and nitric oxide production, which is an inflammatory mediator, and ultimately exhibits an anti-inflammatory effect, thus alleviating atopic dermatitis, It can be used as a food, pharmaceutical, or cosmetic material useful for improvement or treatment.

The effects of the present invention are not limited to the above-mentioned effects. It should be understood that the effects of the present invention include all effects that can be inferred from the following description.

1 is a graph showing the results of confirming the cytotoxicity of each concentration to Raw 264.7 cells of Lactobacillus plantarum LRCC5195 dead cells of the present invention.
2 is Raw 264.7 cells of various saccharide materials, specifically isomaltooligosaccharide, galactooligosaccharide, inulin, sorbitol, and allulose according to an embodiment of the present invention. It is a graph showing the results of confirming the cytotoxicity for each concentration.
3 is a graph showing the results of confirming the change in the expression level of the inflammatory cytokine IL-1β by the Lactobacillus plantarum LRCC5195 dead cells and various saccharide complexes of the present invention.
4 is a graph showing the results of confirming the change in the expression level of the inflammatory cytokine IL-6 by the Lactobacillus plantarum LRCC5195 dead cells and various saccharide complexes of the present invention.
5 is a graph showing the results of confirming the change in the expression level of the inflammatory cytokine TNF-α by the Lactobacillus plantarum LRCC5195 dead cells and various saccharide complexes of the present invention.
6 is a graph showing the results of confirming the inhibition of nitric oxide (NO) production, which is an important mediator in the inflammatory process of Lactobacillus plantarum LRCC5195 dead cells and various sugar complexes of the present invention.

The above objects, other objects, features and advantages of the present invention will be easily understood through the following preferred embodiments in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content may be thorough and complete, and the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

In the present specification, terms such as “comprise” or “have” are intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features It is to be understood that it does not preclude the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof.

In this specification, when a range is described for a variable, the variable will be understood to include all values within the stated range including the stated endpoints of the range. For example, a range of “5 to 10” includes the values of 5, 6, 7, 8, 9, and 10, as well as any subranges such as 6 to 10, 7 to 10, 6 to 9, 7 to 9, etc. It will be understood to include any value between integers that are appropriate for the scope of the recited range, such as 5.5, 6.5, 7.5, 5.5 to 8.5 and 6.5 to 9, and the like. Also for example, a range of "10% to 30%" includes values such as 10%, 11%, 12%, 13%, and all integers up to and including 30%, as well as 10% to 15%, 12% to It will be understood to include any subrange, such as 18%, 20% to 30%, etc., as well as any value between reasonable integers within the scope of the recited range, such as 10.5%, 15.5%, 25.5%, and the like.

Hereinafter, the present invention will be specifically described.

The present invention relates to Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain, isomaltooligosaccharide complex, and uses thereof, and more particularly, having an effect of inhibiting inflammatory cytokine expression and nitric oxide production, which is an inflammatory mediator , It relates to a complex prepared by mixing a novel lactic acid bacterium, Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide, and its use for alleviating or improving atopic dermatitis.

One aspect of the present invention provides a Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P.

The " Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain" is a novel lactic acid bacteria strain isolated from kimchi, deposited on September 23, 2019 at the Korea Microorganism Conservation Center (overseas) with accession number KCCM12591P, the strain is 16S rDNA and contains the nucleotide sequence shown in SEQ ID NO: 1. The strain inhibits the expression of any one of the inflammatory cytokines IL-1β, IL-6, and TNF-α, as well as inhibits the production of nitric oxide (NO), an inflammatory mediator. Accordingly, ultimately, the strain has an anti-inflammatory effect.

In one embodiment of the present invention, the dead cells of the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain isolated and identified from kimchi are inflammatory cytokines IL-1β, IL-6, and expression of any one of TNF-α It was confirmed that it inhibits (FIGS. 3 to 5) and also inhibits the production of nitric oxide (NO), which is an inflammatory mediator (FIG. 6), and thus, it can be seen that the strain ultimately exhibits an anti-inflammatory effect. .

Another aspect of the present invention provides a food composition for alleviating or improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

The Lactobacillus plantarum LRCC5195 strain deposited with the accession number KCCM12591P is as described above.

The term "isomaltooligosaccharides (IMO)" refers to branched oligosaccharides such as isomaltose, isomaltotriose (α-1,6-linked), panose (α-1,6-linked and α-1,4-linked). ) and nigerose (α-1,3-linked) as the main component, it is contained in traditional fermented foods such as rice wine, sikhye, and miso, and is known as a sugar that not only gives sweetness but also umami. In terms of physical properties, IMO is widely used in processed foods because it has superior moisturizing properties compared to sugar or maltose, and is effective in improving sweetness and preventing starch aging. Its main use is mainly for confectionery, beverages, dairy products, and candy, and the effect of improving taste due to imparting umami has been recognized, and its use is expanding to high-quality vinegar and cakes. It has heat resistance and acid resistance, making it easy to use for confectionery, baking, and beverages.

The term "atopic dermatitis" is a recurrent chronic skin disease accompanied by severe itching that begins mainly in infancy or childhood, and is a very common skin disease with a family history. Symptoms begin in infancy and infancy, especially around 2 months of age, and about 50% of them occur before 2 years of age, and most of them appear before 5 years of age. . In some patients, symptoms relieve or heal spontaneously with growth, and more than half of patients who develop in infancy improve before 2 years of age. The shape and distribution of skin lesions are characteristic findings, and it is accompanied by pruritus (itching), dry skin, and characteristic eczema. In infancy, it starts as eczema on the face and on the outstretched side of the limbs, but as you grow older, it characteristically appears in the form of eczema on the bent part of the arm and the bent part behind the knee. In adults, lichenification occurs when the skin at the fold is thickened, and eczema occurs on the face, chest, and nape of the neck other than the extremities compared to infancy and childhood.

The food composition for alleviating or improving atopic dermatitis is not limited thereto, but 10 ⁵ to 10 ⁹ CFU/ml, 10 ⁶ to 10 ⁹ CFU/ml, 10 ⁷ to 10 ^{9 of the Lactobacillus plantarum LRCC5195 strain.} CFU/ml, or 10 ⁸ CFU/ml.

The Lactobacillus plantarum LRCC5195 strain is preferably prepared as a dead cell, but is not limited thereto.

In the present invention, the term "dead cells" refers to a form prepared through heat treatment of a live cell cultured with a strain, and is a form that prevents the activity of the strain from occurring, including antibacterial active substances such as cytoplasm and bacteriocin, organic acids, etc. can do.

The food composition for alleviating or improving atopic dermatitis is not limited thereto, but may further include at least one selected from the group consisting of inulin, galactooligosaccharide, sorbitol and allulose in addition to isomaltooligosaccharide.

The term “inulin” is a storage-type polysaccharide found abundantly in tubers such as the root of chicory or Jerusalem artichoke, and is known as an indigestible water-soluble dietary fiber. Inulin is widely distributed in nature and is included in familiar foods such as grains (wheat, barley) and vegetables (onion, leek, garlic, burdock). The molecular structure of inulin is a linear structure in which 1 to 60 fructose molecules are β(2.1) bonded to a glucose residue, and it is known to act as prebiotics.

The term "galactooligosaccharide" is a generic term for oligosaccharides containing galactose as a main component. Most of them are composed of 2 to 6 sugars and are contained in breast milk or colostrum of milk. It is the only oligosaccharide that uses animal lactose. It is also present in breast milk, is resistant to heat and acid, and is most widely evaluated as a prebiotic.

The term "sorbitol" is a kind of hexahydric alcohol obtained by reducing hexoses such as glucose, and is a substance that gives a sweet taste similar to sugar. Sorbitol is contained in 1~2% of fruits such as pears, peaches, apricots, and grapes, and can be made by reducing glucose with hydrogen. Sorbitol is used not only as a sweetener, but also as an anti-drying agent, a moisture regulator, and a quality stabilizer.

The term "allulose" is a zero-calorie, natural sugar known as a rare sugar, used as a sugar substitute. Allulose is a sweetener found in small amounts in fruits such as wheat and figs as a monosaccharide.

Isomaltooligosaccharide included as an active ingredient in the food composition for alleviating or improving atopic dermatitis, inulin, galactooligosaccharide, sorbitol and allulose, which may be additionally selected in addition to one or more, are saccharide materials that can be used as a substitute for sugar, according to the present invention In the above, each of the sugar materials is included in the composition in the form of a complex prepared by mixing with the dead cells of the Lactobacillus plantarum LRCC5195 strain, which is the novel lactic acid bacterium.

The term "complex" is a new lactic acid bacterium, Lactobacillus plantarum LRCC5195 dead cells of the strain, isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol and allulose in a form in which it is mixed with one oligosaccharide selected from the group consisting of, It can be used in combination with 'mixture' or 'mixture'.

The dead cells and isomaltooligosaccharide complex of Lactobacillus plantarum LRCC5195 strain included as an active ingredient in the food composition for alleviating or improving atopic dermatitis are IL-1β, IL-6, TNF-α, etc. inflammatory cytokine expression and inflammatory mediation By synergistically inhibiting the production of factor NO, it ultimately exhibits an anti-inflammatory effect, and thus can be used as a useful food material for alleviating or improving atopic dermatitis.

In one embodiment of the present invention, the dead cells of the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain, a novel lactic acid bacteria isolated and identified from kimchi, and the isomaltooligosaccharide complex are inflammatory cytokines IL-1β, IL-6, and It was confirmed that the expression of TNF-α and the production of nitric oxide (NO), which is an inflammatory mediator, were significantly inhibited ( FIGS. 3 to 6 ), and accordingly, the strain and the isomaltooligosaccharide complex exhibited excellent anti-inflammatory effects, and ultimately It was found that it could be usefully used in the treatment of atopic dermatitis, a chronic skin inflammatory disease.

When the composition of the present invention is used as a food additive, the dead cells and isomaltooligosaccharide complex of the Lactobacillus plantarum LRCC5195 strain can be added as it is or used with other foods or food ingredients, and can be used appropriately according to a conventional method. have. The mixing amount of the active ingredient may be suitably determined according to the purpose of use (prevention, health or therapeutic treatment), and may further include a food pharmaceutically acceptable food supplement additive. Since the active ingredient of the composition of the present invention is derived from traditional fermented food, there is no problem in terms of stability, so there is no great limitation on the mixing amount.

The food composition of the present invention may include all foods in a conventional sense, and may be used interchangeably with terms known in the art, such as functional food and health functional food.

The term "functional food" as used in the present invention means a food manufactured and processed using raw materials or ingredients useful for the human body according to Health Functional Food Act No. 6727, and "functionality" refers to the structure of the human body. And it refers to ingestion for the purpose of obtaining useful effects for health purposes such as regulating nutrients for function or physiological action.

In addition, the term "health functional food" as used herein refers to a food manufactured and processed using a specific ingredient as a raw material for the purpose of health supplementation or by extracting, concentrating, refining, or mixing a specific ingredient contained in the food raw material, It refers to food designed and processed to sufficiently exert biological control functions such as biological defense, regulation of biological rhythm, prevention and recovery of disease, etc., by the above ingredients, and the composition for health food is used for prevention of diseases and prevention of diseases. It can perform functions related to recovery, etc.

There is no limitation on the kind of food in which the composition of the present invention can be used. In addition, the composition comprising the dead cells of the Lactobacillus plantarum LRCC5195 strain of the present invention and the isomaltooligosaccharide complex as active ingredients can be prepared by mixing appropriate other auxiliary ingredients that may be contained in food and known additives according to the selection of those skilled in the art. can Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and There are vitamin complexes and the like, and it can be prepared by adding the complex according to the present invention as a main component to juice, tea, jelly, juice, and the like.

In addition, foods that can be applied to the present invention include, for example, special nutritional foods (eg, formula milk, infant food, etc.), processed meat products, fish meat products, tofu, jelly, noodles (eg, ramen, noodles, etc.), health supplements , seasoned foods (eg soy sauce, soybean paste, red pepper paste, mixed soy sauce, etc.), sauces, sweets (eg snacks), dairy products (eg fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.) ), beverages (eg, fruit, vegetable beverages, soy milk, fermented beverages, etc.), and natural seasonings (eg, ramen soup, etc.).

When the health functional food composition of the present invention is used in the form of a beverage, it may contain various sweetening agents, flavoring agents, or natural carbohydrates as additional ingredients, as in a conventional beverage. In addition to the above, the health functional food composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin , alcohol, a carbonation agent used in carbonated beverages, and the like. In addition, it may contain the pulp for the production of natural fruit juice, fruit juice beverage and vegetable beverage.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

The Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide deposited with the accession number KCCM12591P, and the anti-inflammatory effect and ultimately atopic dermatitis treatment effect by the complex thereof are as described above.

The pharmaceutical composition for preventing or treating atopic dermatitis of the present invention may further include conventional pharmaceutically acceptable carriers and/or excipients in addition to the active ingredients, in addition to pharmaceuticals such as binders, disintegrants, coating agents, lubricants, etc. It can be formulated and prepared with various additives commonly used.

The pharmaceutical composition for preventing or treating atopic dermatitis of the present invention is in the form of powder, granule, tablet, capsule or liquid by mixing the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide complex with an appropriate carrier, excipient, auxiliary active ingredient, etc. can be formulated as In addition, the pharmaceutical composition can be enteric-coated so that it passes through the stomach and reaches the small intestine, and the microorganism, which is an active ingredient, is rapidly released into the intestine by using a known method.

Excipients usable in the present invention include sugars such as sucrose, lactose, mannitol, glucose, and the like, and starches such as corn starch, potato starch, rice starch, and partially pregelatinized starch. The binder may include polysaccharides such as dextrin, sodium alginate, carrageenan, guar gum, acacia, agar, etc., naturally-occurring macromolecular substances such as tragacanth, gelatin, gluten, hydroxypropylcellulose, methylcellulose, hydroxypropylmethyl Cellulose derivatives such as cellulose, ethyl cellulose, hydroxypropyl ethyl cellulose, carboxymethyl cellulose sodium, polyvinylpyrrolidone, polyvinyl alcohol, polyvinyl acetate, polyethylene glycol, polyacrylic acid, polymethacrylic acid, and vinyl acetate resin contains polymers.

Disintegrants include cellulose derivatives such as carboxymethyl cellulose, carboxymethyl cellulose calcium, low-substituted hydroxypropyl cellulose, sodium carboxymethyl starch, hydroxypropyl starch, corn starch, potato starch, rice starch and partially pregelatinized starch, etc. of starch can be used.

Examples of lubricants usable in the present invention include talc, stearic acid, calcium stearate, magnesium stearate, colloidal silica, hydrosilicone dioxide, various types of waxes and hydrogenated oils.

As a coating agent, dimethylaminoethylmethacrylate-methacrylic acid copolymer, polyvinylacetaldiethylaminoacetate, ethylacrylate-methacrylic acid copolymer, ethylacrylate-methylmethacrylate-chlorotrimethylammoniumethylmethacrylate Water-insoluble polymer such as copolymer, ethyl cellulose, methacrylic acid-ethyl acrylate copolymer, hydroxypropylmethylcellulose phthalate, hydroxypropylmethylcellulose acetate succinate, and the like, and methylcellulose, hydroxypropylmethylcellulose , polyvinylpyrrolidone, water-soluble polymers such as polyethylene glycol, but is not necessarily limited thereto.

The dosage of the dead cells of the Lactobacillus plantarum LRCC5195 strain, which is an active ingredient in the pharmaceutical composition for preventing or treating atopic dermatitis of the present invention, is for the purpose of treatment or prevention, the type of patient to be treated or prevented, symptoms, weight, and age of the patient. It may be appropriately determined in consideration of age or gender. ^{Although not limited thereto, 10 5} to 10 ⁹ CFU / ml, 10 ⁶ to 10 ⁹ CFU / ml, 10 ⁷ to 10 ⁹ CFU / ml, or 10 ⁸ CFU / ml of the dead cells of the Lactobacillus plantarum LRCC5195 strain The concentration may be administered once or in divided doses as needed.

As used herein, the term “administration” refers to introducing a predetermined substance into an individual by an appropriate method, and the administration route of the composition may be administered through any general route as long as it can reach a target tissue. Intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, intrapulmonary administration, may be administered intrarectally, but is not limited thereto.

The term “individual” refers to all animals including humans, rats, mice, livestock, and the like. Preferably, it may be a mammal including a human.

In one embodiment of the present invention, the complex prepared by mixing the dead cells of the Lactobacillus plantarum LRCC5195 strain, a novel lactic acid bacteria isolated and identified from kimchi and isomaltooligosaccharide, is a strain or isomaltooligosaccharide alone, compared to the strain or isomaltooligosaccharide alone, inflammatory cytokine expression and inflammation It was confirmed that it has a synergic effect in inhibiting the production of nitric oxide, which is a mediator ( FIGS. 3 to 6 ).

That is, the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide complex significantly inhibits inflammatory cytokine expression and nitric oxide production, which is an inflammatory mediator, and ultimately exhibits an anti-inflammatory effect. It can be used as a useful food, pharmaceutical, or cosmetic material.

Another aspect of the present invention provides a cosmetic composition for improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

The Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide deposited with the accession number KCCM12591P, and the anti-inflammatory effect and ultimately atopic dermatitis improvement or therapeutic effect by the complex thereof are as described above.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, and skin, lotion, cream, essence, emulsion, gel, hand cream, lipstick, cleansing foam, cleansing cream, cleansing water, spray , shampoo, conditioner, treatment, body cleanser, soap, pack, massage agent, face powder, compact, foundation, two-way cake, and can be formulated in the form of a makeup base.

In addition, the cosmetic composition may additionally contain fatty substances, organic solvents, solubilizers, thickeners and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non-ionic emulsifiers, fillers , sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredient commonly used in cosmetic compositions. It may contain adjuvants.

The composition of the present invention uses a composition comprising, as active ingredients, Lactobacillus plantarum LRCC5195 strain, a novel lactic acid bacteria derived from kimchi, a traditional fermented food, and isomaltooligosaccharide as active ingredients, even when used as a food composition, pharmaceutical composition or cosmetic composition. Since it may have fewer side effects compared to general synthetic compounds, it can be safely included and usefully used.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1: Isolation and identification of Lactobacillus plantarum LRCC5195 strain

Example 1-1: Isolation of lactic acid bacteria strain from kimchi

Sterile distilled water was added to a sample collected from kimchi, a traditional fermented food, to make a diluted solution, and then the inside of the sample was homogenized with a grinding homogenizer (Stomacher, Pro-Media SH-001, ELMEX). Using sterile saline, the diluted solution was diluted step by step by a 10-fold dilution method, and then 0.1 ml was taken. This was smeared on MRS solid medium supplemented with 0.002% by weight of BCP (Bromocresol purple) and 1.5% by weight of agar, and cultured for 48 hours in an incubator maintained at 37°C. After culturing, colonies showing yellow rings were selected and subcultured 2-3 times in the same medium for each individual to purely isolate each strain. Separated strains were inoculated into MRS broth for each individual, cultured at 37°C for 48 hours, and then used while refrigerated at 4°C.

Example 1-2: Identification of Lactobacillus plantarum LRCC5195 strain

The 16S rDNA gene base sequence of the strain isolated in Example 1-1 was analyzed through 16S rDNA sequence analysis to identify the isolated strain. 16S rDNA gene nucleotide sequence was the same as SEQ ID NO: 1, and as a result of nucleotide sequence analysis, the strain was confirmed to have a maximum of 99% homology with the Lactobacillus sp. The identified new Lactobacillus plantarum strain was named Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195), and was deposited with the Korea Microbial Conservation Center on September 23, 2019 and was given an accession number KCCM12591P.

Example 2: Evaluation of cytotoxicity of dead cells and various sugars of Lactobacillus plantarum LRCC5195 strain

Prior to utilizing the dead cells and saccharide complex of the Lactobacillus plantarum LRCC5195 strain for the purpose of preventing or treating atopy, the cytotoxicity of the dead cells and the saccharide complex of the Lactobacillus plantarum LRCC5195 strain was first evaluated.

Dead cells of Lactobacillus plantarum LRCC5195 strain and each saccharide material, specifically, isomaltooligosaccharide (IMO), galactooligosaccharide, inulin, sorbitol, and allulose cells Safety at the actual cellular level was confirmed by measuring toxicity by performing an MTT assay.

As animal cells, Raw 264.7 (KCLB40071) mouse macrophages purchased from KCLB (Korean Cell Line Bank) were used. As a medium for cell culture, DMEM (Dulbecco's Modified Eagle's Medium, HyClone) medium containing 100 unit/ml penicillin and 100 μg/ml streptomycin (Penicillin-Streptomycin Solution, HyClone) and 10% FBS (HyClone) was used. Cells were cultured in an environment of 37° C., 5% CO _{2 .}

Specifically, Raw 264.7 cells were aliquoted in a 12-well plate at a concentration of ^{1×10 5} cells/well (cells/well), and cultured in an environment of _{37° C., 5% CO 2 for 24 hours.} Thereafter, the medium was replaced with a fresh DMEM medium containing 5% FBS and cultured in the same environment for 24 hours. The dead cells of the LRCC5195 strain were cultured at a strain concentration of 1×10 ⁹ CFU/ml, centrifuged, suspended in PBS, and then autoclaved to prepare dead cells. Dead cells were treated with cells to 1×10 ⁸ CFU/well and cultured for 24 hours. Each of the various saccharide materials was dissolved in PBS to 100 mg/ml, and then the cells were treated to 10 mg/well and cultured for 24 hours. In the case of dead cells and saccharide mixture, it ^{was treated with 1×10 8} CFU/well of dead cells and 10 mg/well of saccharide material in the same manner.

To measure the viability of the Raw 264.7 cell line, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reagent was used, and a microplate reader (model Infinite ^® F200, Tecan, Mannedorf, Switzerland) was used to measure the absorbance at a wavelength of 590 nm and calculate the cell viability (%).

Cell viability (%) = (absorbance of the group treated with the strain or saccharide culture medium / absorbance of the group not treated with the strain or saccharide culture medium) × 100

As a result of measuring the degree of cytotoxicity by concentration of Lactobacillus plantarum LRCC5195 dead cells to Raw 264.7 cells by performing MTT assay, as shown in FIG. 1, LRCC5195 dead cells 1 × 10 ⁸ CFU / ml, 1 When treated with ×10 ⁷ CFU / ㎖, 1 × ¹⁰⁶ CFU / ㎖, it was confirmed that there is no significant change in the viability of Raw 264.7 cells. Therefore, in the case of LRCC5195 dead cells, it ^{was found that even if the cells were treated up to 10 8} CFU/ml, it did not induce toxicity to the cells.

In addition, various saccharide materials for each type, i.e., isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose, were treated in Raw 264.7 cells at a concentration of 10 mg/ml to measure the degree of cytotoxicity. As shown, it was confirmed that there was no significant change in the viability of Raw 264.7 cells when treated with isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose. Therefore, it was found that 10 mg/ml of isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose did not induce cell toxicity.

Through the above results, it was confirmed that the dead cells of the Lactobacillus plantarum LRCC5195 strain and various sugars, that is, isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose do not show cytotoxicity, the Lactobacillus plan of the present invention It was found that the complex mixed with the dead cells of the Tarum LRCC5195 strain and various saccharide materials can be safely used for the purpose of preventing or treating atopy.

Example 3: Evaluation of atopic dermatitis treatment efficacy of dead cells and saccharide complex of Lactobacillus plantarum LRCC5195 strain

Inflammation in the skin has many different meanings. The basic physiological mechanism of atopic skin disease, acne-prone skin disease, and sensitive skin disease undergoes an inflammatory process, and a representative disease among them is atopic dermatitis (AD). AD is a chronic inflammatory disease accompanied by itching and inflammation. Most of the patients are characterized by elevated blood IgE levels, and macrophages activated in this inflammatory response are NO (nitric oxide) and TNF-α (tumor necrosis factor). -α), IL-6 (interleukin-6), IL-1β, etc. are released.

Accordingly, in the following, the dead cells of the Lactobacillus plantarum LRCC5195 strain and the saccharide complex In order to verify the improvement and therapeutic effect of atopic dermatitis, the production of inflammatory cytokines IL-1β, IL-6, TNF-α and NO, an inflammatory factor, in Raw 264.7 cell culture medium of macrophages were measured.

Example 3-1: Evaluation of inflammatory cytokine inhibitory ability of dead cells and saccharide complex of Lactobacillus plantarum LRCC5195 strain

To evaluate the anti-inflammatory efficacy of the dead cells of the Lactobacillus plantarum LRCC5195 strain and each saccharide material (isomaltooligosaccharide, galactooligosaccharide, inulin, sorbitol, allulose) complex, ELISA (enzyme-linked immunosorbent assay) was performed to The production amount of cytokines IL-1β, IL-6, TNF-α was measured.

Specifically, Raw 264.7 cells were cultured, aliquoted in a 12-well plate at a concentration of ^{1Х10 5 cells/well, and cultured for 24 hours.} To induce inflammation, LPS was added to 2.5 μg/ml in DMEM medium containing 5% FBS. Lactobacillus plantarum LRCC5195 dead cells were cultured at a strain concentration of 1×10 ⁹ CFU/ml, centrifuged, suspended in PBS, and then autoclaved to prepare dead cells. Dead cells were treated with cells to 1×10 ⁸ CFU/well and cultured for 24 hours. Each of the various saccharide materials was dissolved in PBS to 100 mg/ml, and then the cells were treated to 10 mg/well and cultured for 24 hours. In the case of dead cells and saccharide mixture, it ^{was treated with 1×10 8} CFU/well of dead cells and 10 mg/well of saccharide material in the same manner. When performing ELISA, use IL-1β mouse ELISA kit (Invitrogen, USA), IL-6 mouse ELISA kit (Invitrogen, USA), and TNF-α mouse ELISA kit (Invitrogen, USA) for each inflammatory cytokine to be measured. and measurements were performed according to the manufacturer's guidelines.

Changes in the expression level of the inflammatory cytokine IL-1β

Dead cells of the Lactobacillus plantarum LRCC5195 strain and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) were treated with Raw 264.7 cells alone or in a mixture, and As a result of measuring the change in the expression level of IL-1β, as shown in FIG. 3 , when LPS, known as an inflammatory substance, was treated and Lactobacillus plantarum LRCC5195 dead cells were treated alone, the expression level of IL-1β was very high. appeared to be low. It was found that there was almost no induction of inflammation by the Lactobacillus plantarum LRCC5195 dead cells.

In addition, it was confirmed that the expression level of IL-1β was very low even when isomaltooligosaccharide, inulin, and galactooligosaccharide among various saccharide materials were administered alone. In contrast, when sorbitol and allulose were administered alone, it was confirmed that there was no significant reduction effect on IL-1β expression.

In addition, when Lactobacillus plantarum LRCC5195 dead cells and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) were treated in combination, a significant decrease in IL-1β expression was confirmed in all experimental groups. became In particular, among these, when Lactobacillus plantarum LRCC5195 dead cells and each isomaltooligosaccharide, inulin, and galactooligosaccharide were treated in a complex manner, similar to the case where each saccharide material was treated alone, IL-1β expression was more pronounced It was confirmed that it shows a decreasing trend.

Therefore, through the above results, it was confirmed that the Lactobacillus plantarum LRCC5195 dead cells and the complex mixed with isomaltooligosaccharide, inulin, and galactooligosaccharide among various saccharide materials significantly inhibited the expression of IL-1β, an inflammatory cytokine, It was found that it can be usefully used as an anti-inflammatory treatment candidate for atopic dermatitis.

Changes in the expression level of the inflammatory cytokine IL-6

The expression level of IL-6 among inflammatory cytokines by treating Raw 264.7 cells with dead cells of Lactobacillus plantarum LRCC5195 strain and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) alone or in combination As a result of measuring the change, as shown in FIG. 4 , when LPS, known as an inflammatory substance, was treated and Lactobacillus plantarum LRCC5195 dead cells were treated alone, IL- corresponding to about 55% level compared to LPS alone treatment 6 numbers were found. However, when each saccharide material, ie, isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose, was treated alone, it was not possible to confirm the decrease in the expression level of IL-6 in most samples.

On the other hand, when Lactobacillus plantarum LRCC5195 dead cells and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) were treated in combination, there was a marked decrease in IL-6 expression in all experimental groups. has been confirmed

In the case of a complex treated with Lactobacillus plantarum LRCC5195 dead cells and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose), 1×10 ⁸ CFU/well of Lactobacillus plantarum LRCC5195 The cells and 10 mg/well of each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) are mixed in the same amount at a weight ratio of 1:1 (50% by weight each), and IL-6 expression If it is assumed that the amount reduction is only the effect of the Lactobacillus plantarum LRCC5195 dead cells alone, it was expected that the decrease in the expression level of IL-6 would be higher when mixed with each saccharide material compared to treatment with the Lactobacillus plantarum LRCC5195 dead cells alone.

However, as shown in FIG. 4 , substantially the Lactobacillus plantarum LRCC5195 dead cells and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) were mixed and the expression level of IL-6 during complex treatment was rather It was confirmed that it showed a lower decreasing trend. Based on this, it was found that when the Lactobacillus plantarum LRCC5195 dead cells and each saccharide material were mixed and treated with each other, there was a clear synergy in reducing the expression level of IL-6.

Therefore, even through the above results, it was confirmed that the Lactobacillus plantarum LRCC5195 dead cells and the complex mixed with isomaltooligosaccharide, inulin, and galactooligosaccharide among various saccharide materials significantly inhibited the expression of IL-6, an inflammatory cytokine, It was found that it can be usefully used as an anti-inflammatory treatment candidate for atopic dermatitis.

Changes in the expression level of the inflammatory cytokine TNF-α

Dead cells of Lactobacillus plantarum LRCC5195 strain and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) were treated with Raw 264.7 cells alone or in combination to the expression level of TNF-α among inflammatory cytokines As a result of measuring the change, as shown in FIG. 5 , when LPS, known as an inflammatory substance, was treated and Lactobacillus plantarum LRCC5195 dead cells were treated alone, the expression level of TNF-α was significant compared to the LPS alone treatment group. It was confirmed that there was a slight decrease with no difference.

On the other hand, when each saccharide material, that is, isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose, was treated alone, it was confirmed that the TNF-α level decreased compared to the LPS alone treatment group in most of each saccharide material. became It was confirmed that the most significantly decreased in galactooligosaccharide among each saccharide material.

However, when the dead cells of the Lactobacillus plantarum LRCC5195 strain and each saccharide material were mixed and administered in combination, as shown in FIG. 5, when the dead cells of the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide were administered in combination It was confirmed that the TNF-α level was most significantly reduced.

On the other hand, in the case of complex treatment by mixing other saccharide materials other than isomaltooligosaccharide and dead cells of the Lactobacillus plantarum LRCC5195 strain, the maximum TNF-α expression level shown in the LPS-only treatment group was similarly compared to the LPS-only treatment group. was found to increase.

Therefore, through the above results, it was confirmed that the complex mixed with Lactobacillus plantarum LRCC5195 dead cells and isomaltooligosaccharide among various sugar materials significantly inhibited the expression of TNF-α, an inflammatory cytokine, for anti-inflammatory treatment for atopic dermatitis It was found that it can be usefully used as a candidate material.

Example 3-2: Evaluation of NO inhibitory ability of dead cells and saccharide complexes of Lactobacillus plantarum LRCC5195 strain

To evaluate the anti-inflammatory efficacy of the dead cells of the Lactobacillus plantarum LRCC5195 strain and each saccharide material (isomaltooligosaccharide, galactooligosaccharide, inulin, sorbitol, allulose) complex, NO assay was performed to evaluate the inflammatory mediator NO ( nitric oxide) was measured.

Specifically, NO was measured using the cell culture medium used for the inflammatory cytokine analysis of Example 3-1. NO production was measured using the Griess reagent system (Promega, USA), and was performed according to the manufacturer's guidelines.

Dead cells of Lactobacillus plantarum LRCC5195 strain and each saccharide material (isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, allulose) were treated alone or in combination in Raw 264.7 cells to reduce the expression level of NO, an inflammatory mediator, As a result of measuring by performing the NO assay, as shown in FIG. 6 , when LPS, known as an inflammatory substance, was treated and Lactobacillus plantarum LRCC5195 dead cells were treated alone, the expression level of NO compared to the LPS alone treatment group It was confirmed that there was a slight decrease without a significant difference.

On the other hand, when each saccharide material, that is, isomaltooligosaccharide, inulin, galactooligosaccharide, sorbitol, and allulose, is treated alone, LPS alone in each isomaltooligosaccharide, galactooligosaccharide, inulin and allulose except for sorbitol It was confirmed that the NO level decreased compared to the treatment group. It was confirmed that the most significantly decreased in galactooligosaccharide among each saccharide material.

In addition, when the dead cells of the Lactobacillus plantarum LRCC5195 strain and each saccharide material were mixed and administered in combination, as shown in FIG. 6, the dead cells of the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide or galactooligosaccharide were combined It was confirmed that the NO level was most significantly reduced when administered as

On the other hand, in the case of complex treatment by mixing the dead cells of the Lactobacillus plantarum LRCC5195 strain with other saccharide materials other than isomaltooligosaccharide and galactooligosaccharide, the NO expression level shown in the LPS alone treatment group is similar to that of the LPS alone treatment group was found to increase significantly.

Therefore, through the above results, it was confirmed that the Lactobacillus plantarum LRCC5195 dead cells and the complex mixed with isomaltooligosaccharide and galactooligosaccharide among various sugar materials significantly inhibited the expression of NO, an inflammatory mediator, for atopic dermatitis. It was found that it can be usefully used as an anti-inflammatory treatment candidate.

Example 4: Selection of candidate materials for dead cells and saccharide complexes of Lactobacillus plantarum LRCC5195 strain for the treatment of atopic dermatitis

The cytotoxicity, inflammatory cytokines, and NO measurement results analyzed in Examples 2 and 3 are summarized in Table 1 below.

In the selection of anti-inflammatory treatment candidates for atopic dermatitis, the dead cells of Lactobacillus plantarum LRCC5195 strain and each saccharide complex were selected. As a result of the final analysis of cell viability and the decrease in the levels of inflammatory cytokines (IL-1β, IL-6, TNF-α) and inflammatory mediators (NO), the dead cells of the Lactobacillus plantarum LRCC5195 strain and isoforms in the sugar material In the case of the maltooligosaccharide-mixed complex, it was found that the production of inflammatory cytokines and inflammatory mediators was consistently reduced, and the anti-inflammatory effect was excellent.

Accordingly, it was found that the complex in which the dead cells of the Lactobacillus plantarum LRCC5195 strain and isomaltooligosaccharide were mixed could be usefully used as an anti-inflammatory treatment material for atopic dermatitis.

LRCC5195 dead cells alone LRCC5195 dead cells + each sugar material complex isomaltooligosaccharide inulin galacto
oligosaccharide sorbitol allulose Cytotoxicity (MTT) ¹⁾ No cytotoxicity Reduced inflammatory cytokines and inflammatory mediators compared to LPS-treated group
Whether ²⁾ IL-1β ○ ○ ○ ○ ○ ○ IL-6 ○ ○ ○ ○ ○ ○ TNF-α ○ ○ × × ○ ○ NO ○ ○ × ○ × ×

^{1) If the} cell viability is 80% or more, it is determined that there is no cytotoxicity;

²⁾ ○, × are determined according to whether the number has decreased.

Korea Microorganism Conservation Center (Overseas) KCCM12591P 20190923

<110> LOTTE CHILSUNG BEVERAGE CO LTD <120> A mixture of Lactobacillus plantarum LRCC5195 and isomaltooligosaccharide having ameliorating or improving atopic dermatitis <130> DP-2019-0312 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1314 <212> DNA <213> Artificial Sequence <220> <223> LRCC5195_16S rDNA <400> 1 ttggttgcta gcgtagagcg tgatagtggc tcgaggtctg gtaatgatgt gagcgcgttg 60 catcatgaat tacatttgag tgagtggcga actggtgagt aacacgtggg aaacctgccc 120 agaagcgggg gataacacct ggaaacagat gctaataccg cataacaact tggaccgcat 180 ggtccgagtt tgaaagatgg cttcggctat cacttttgga tggtcccgcg gcgtattagc 240 tagatggtga ggtaacggct caccatggca atgatacgta gccgacctga gagggtaatc 300 ggccacattg ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt 360 ccacaatgga cgaaagtctg atggagcaac gccgcgtgag tgaagaaggg tttcggctcg 420 taaaactctg ttgttaaaga agaacatatc tgagagtaac tgttcaggta ttgacggtat 480 ttaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag 540 cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg ttttttaagt ctgatgtgaa 600 agccttcggc tcaaccgaag aagtgcatcg gaaactggga aacttgagtg cagaagagga 660 cagtggaact ccatgtgtag cggtgaaatg cgtagatata tggaagaaca ccagtggcga 720 aggcggctgt ctggtctgta actgacgctg aggctcgaaa gtatgggtag caaacaggat 780 tagataccct ggtagtccat accgtaaacg atgaatgcta agtgttggag ggtttccgcc 840 cttcagtgct gcagctaacg cattaagcat tccgcctggg gagtacggcc gcaaggctga 900 aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc 960 tacgcgaaga accttaccag gtcttgacat actatgcaaa tctaagagat tagacgttcc 1020 cttcggggac atggatacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg 1080 ggttaagtcc cgcaacgagc gcaaccctta ttatcagttg ccagcattaa gttgggcact 1140 ctggtgagac tgccggtgac aaaccggagg aaggggggga tgacgtcaaa tcatcatgcc 1200 ccttatgacc tgggctaccc ccgtgctaca agggatggta aaccaattgc gaactcgcga 1260 gagaaagcta attccttaaa ccattttccg ttcggattgg aggtggaaat cgcc 1314

Claims (10)

Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and comprising the 16S rDNA nucleotide sequence of SEQ ID NO: 1.
According to claim 1, wherein the strain inhibits the expression of any one of the inflammatory cytokines IL-1β, IL-6, and TNF-α, and inhibits the production of the inflammatory mediator NO, the strain.
The strain according to claim 1, wherein the strain has an anti-inflammatory effect.
delete A food composition for alleviating or improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.
The food composition of claim 5, wherein the composition contains the dead cells of the Lactobacillus plantarum LRCC5195 strain at ^{a concentration of 10 5} to 10 ⁹ CFU/ml.
The food composition of claim 5, wherein the composition further comprises at least one selected from the group consisting of inulin, galactooligosaccharide, sorbitol and allulose.
According to claim 5, wherein the composition inhibits the expression of any one of the inflammatory cytokines IL-1β, IL-6 and TNF-α, and inhibits the production of inflammatory mediator NO, atopic dermatitis alleviation or improvement food composition.
A pharmaceutical composition for preventing or treating atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.
A cosmetic composition for improving atopic dermatitis, comprising the Lactobacillus plantarum LRCC5195 (Lactobacillus plantarum LRCC5195) strain deposited with accession number KCCM12591P and isomaltooligosaccharide as active ingredients.

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