KR102243579B1 - Functional foods for lymphatic circulation promotion - Google Patents

Abstract

Disclosed is a functional food composition that promotes lymphatic circulation in the body, improves the environment around cells, and helps to restore normal cell metabolism. The functional food composition for promoting lymphatic circulation according to the present invention includes cheonggung, maple syrup, crust, tabasheer, haedongpi and uiyiin in powder, liquid or extract form.

Description

Functional food composition for promoting lymphatic circulation {FUNCTIONAL FOODS FOR LYMPHATIC CIRCULATION PROMOTION}

The present invention relates to a functional food composition for promoting lymphatic circulation, and more particularly, a functional food composition for promoting lymphatic circulation that can help improve the environment around cells by promoting lymphatic circulation in the body and restore normal metabolism of cells. It is about.

The inventors of the present invention have found that smooth lymph circulation plays an important role in maintaining a healthy body condition and rapidly recovering a diseased body through numerous clinical experiences.

Bodily fluid circulation includes both blood circulation and lymph circulation and is responsible for the physical circulation of substances in the body. Blood circulation plays a central role in the circulation of substances in the body, and lymph circulation plays an auxiliary role in helping the circulation of substances in the cardiovascular system.

The lymphatic system is composed of lymphatic fluid, lymphatic vessels, and lymph nodes. They are responsible for returning fluids that may exist excessively between the tissues of the human body back to the heart. In addition, it plays a role of sending waste products left after cell metabolism from tissues and cell metabolites such as fats and proteins with a large molecular weight that cannot pass through capillaries through the vena cava into the blood. Capillary lymphatic vessels exist adjacent to the capillaries and are distributed throughout the body. The tissue fluid in the tissue becomes lymph fluid as it enters the capillary lymphatic vessels. When body fluid enters the lymphatic vessel, it does not pass through the membrane, unlike capillaries, but through small holes at the end of the capillary lymphatic vessel. Therefore, substances having a large molecular weight that cannot pass through the capillary membrane can easily pass through, and most of the components of tissue fluid are included in the lymph fluid. The distal part of the capillary lymphatic vessel has a structure that makes it difficult for the body fluid that has entered the lymphatic vessel to escape. The composition of lymph fluid is similar to that of tissue fluid, and consists of water, dead cells, fat and protein, salt, sugar, urea, and lymphocytes. Lymphatic vessels are the pathways that carry lymph fluid from tissues to the vena cava. Lymphatic vessels are divided into shallow lymphatic vessels located between the skin and fascia and deep lymphatic vessels located below the fascia, depending on their location. Most of them are made up of shallow lymphatic vessels. Therefore, the superficial lymphatic vessel plays an important role in lymphatic circulation, and it is possible to promote lymphatic circulation through a lymphatic massage that physically stimulates the skin. The movement of lymph fluid is mainly caused by a small power generated by the connection of the valves in the lymphatic vessels and the contraction and relaxation movements of the muscles. When the muscle contracts, the volume inside the lymphatic vessel decreases as the lymphatic vessel is partially pressed by the thickened muscle. At this time, the lymph fluid does not go to the direction blocked by the valve as much as the volume of the lymphatic vessel is reduced, but moves toward the heart. On the contrary, when the muscles are relaxed, the volume inside the lymphatic vessels increases as the lymphatic vessels that were pressed partially become thinner. At this time, the lymph fluid must be replenished by the increased volume, but it cannot come from the direction of the heart blocked by the valve, but moves away from the heart to fill it. Therefore, one section of the valve acts as a small heart-like pump that helps lymphatic circulation according to the movement of the muscles. In addition, since a number of these small pumps are connected in series, muscle movements in long sections such as arms and legs act as a power source that strongly promotes the movement of lymph fluid. Capillary lymphatic vessels closer to the skin than capillaries are susceptible to infection by pathogenic microorganisms such as bacteria and viruses even with minor skin damage. To compensate for this, the lymph nodes are present before the lymphatic vessels reach the vena cava. Lymph nodes have a mesh structure, and the internal mesh structure can be well blocked, so they are mainly distributed under the ears, under the tongue, under the tongue, under the armpits, and in the groin, where there is a lot of movement or large muscle movement. Lymph nodes produce lymphocytes to protect the body from invasion by pathogens, and filter, break down and remove pathogens such as bacteria and viruses, and dead cells and waste products in lymph fluid before returning to the blood. The movement of lymph fluid begins when tissue fluid enters the capillary lymphatic vessels. The capillary lymphatic vessels join the lymphatic vessels, and the lymphatic vessels contain lymphatic valves that block the backflow of lymph fluid like valves in a vein and create a circulating power for lymphatic fluid. Again, the lymph fluid passes through the lymph nodes, which are responsible for preventing microbial invasion, and merges with the blood in the vena cava. Thus, lymph circulation begins at the tip of the fingertips, toes and head and proceeds toward the heart, then passes through the lymph nodes in the armpits, groin, under the ears and under the tongue to filter pathogenic microorganisms and waste products, merges with the vena cava and enters the vena cava. This is the circulation of bodily fluids.

As such, the lymphatic system is closely related to the cardiovascular system and is an auxiliary circulatory system that aids in body fluid circulation. When substances are exchanged between capillaries and tissues, structurally, the amount of body fluid that flows from the capillaries into the tissue is always greater than the amount that enters the capillaries from the tissue. At this time, if the remaining body fluid is accumulated as it is, swelling occurs in the tissue and the hydrostatic pressure increases. In addition, blood loses moisture due to insufficient body fluids, making it dry and sticky. If these phenomena are not improved and persist, serious fluid circulation disorders are caused, leading to many problems. The lymphatic system plays a role in improving such imbalance by allowing fluids accumulated in tissues to move to the vena cava through lymphatic vessels, which are channels through which fluids can move from tissues to vena cava. Thus, edema in the human body means a disorder of fluid circulation, and a disorder of fluid circulation means a disorder of lymphatic circulation. In addition, whether improvement of systemic edema or local edema, improvement of human body edema means improvement of fluid circulation disorder, and improvement of fluid circulation disorder means improvement of lymphatic circulation disorder.

Lymphatic circulation is the process of collecting stagnant body fluids from every corner of the body and returning them to the blood through the vena cava. In the process, it promotes the discharge of waste products and allows the recycling of what is needed. From the perspective of simply bodily fluid circulation, blood circulation is more important than lymph circulation, but from the perspective of tissue environment that affects cell metabolism, lymph circulation, which is directly involved in the circulation of tissue fluid, is more important than blood circulation. Lymphatic circulation first aids blood circulation in the cardiovascular system and promotes the supply of nutrients, moisture, oxygen, hormones, etc., which are necessary substances for cells, and at the same time quickly removes waste products, toxic substances, and inflammatory substances accumulated in tissues or cells. It helps to create an optimal environment for cells to function normally. In addition, it helps the absorption and movement of ingested medicines or nutrients, and increases the effect by quickly delivering them to the necessary areas. As described above, lymph circulation plays a large role in creating the optimal conditions for cells to function, so it can be said to be an important factor for healthy human body activity.

Lymphatic circulation disorder causes systemic swelling or localized swelling because body fluid accumulated in tissues cannot return to blood through lymphatic circulation and remains in the tissues. In other words, it can be concluded that both systemic edema or local edema of the body is a lymphatic circulatory disorder. If such edema is short-term, it is not a big problem, but if it lasts for a long time, it lowers the metabolic function of the affected area and acts as a cause of many diseases. Considering that muscle movement is the only driver of lymph circulation, all symptoms that can occur due to lack of exercise are symptoms of lymphatic circulation disorder, and all the effects that can be improved by exercise are the effects that can be obtained by promoting lymph circulation. Lymphatic circulation acquires circulatory power only through muscle movement, and the reality is that the modern man, who has less need for movement for survival, lacks the opportunity for muscle movement, so that lymphatic circulation is not smoothly performed. Therefore, it is urgent to find a method for promoting lymph circulation for modern people who have a lack of fluid circulation due to an absolute lack of exercise. Nevertheless, although a lot of discussions have been made on blood circulation and a lot of countermeasures have been developed, it is true that the discussion of lymph circulation is insufficient compared to its importance. The concept of lymphatic circulation has not yet been recognized as medically important compared to its practical importance, and the development of medicines is also insufficient.

An object of the present invention is to provide a functional food composition that can help improve the environment around cells by promoting lymphatic circulation in the body and restore normal metabolism of cells.

Another object of the present invention is to provide a functional food composition capable of improving various symptoms that may occur due to lymphatic circulation disorders.

The above-described problems of the present invention can be solved by a functional food composition comprising cinnamon, maple syrup, crust, tabasheer, haedongpi, and uiyiin in powder, liquid or extract form.

The functional food composition may be made into pills, granules, or powders for oral administration by mixing maple syrup and tabasheer in a mixed powder of cheonggung, crust, haedongpi and uiyiin.

The functional food composition is a liquid or powder extract extracted with water, ethanol, or water and ethanol from the mixed powder of Chungoong, Crust, Haedongpi, and Uiiin, and maple syrup and tabasheer are mixed to form pills, granules, powders or It can be made liquid.

The functional food composition includes, as an active ingredient, an extract extracted with water, ethanol, or water and ethanol by mixing cinnamon, maple syrup, crust, tabasheer, haedongpi, and uiyiin at a certain ratio, as an active ingredient, as an active ingredient, a pill, granule, powder or It can be made liquid.

The functional food composition according to the present invention basically improves the environment around cells by making lymphatic circulation smooth in the body, helps to restore normal metabolism of cells, and creates an environment so that cells in the body can function normally. give. Directly, by promoting lymphatic circulation in the body, it exhibits effects such as improving edema, improving inflammation and pain, removing bruises, controlling body temperature, improving cellular environment, and promoting drug delivery.

1) The effect of improving edema: The promotion of lymphatic circulation according to the present invention basically circulates the body fluid accumulated in the tissue through the lymphatic vessels so that it merges with the blood in the vena cava, and through this, the body fluid is included in the systemic circulation. The effect is the improvement of edema. Edema occurs due to stagnation of body fluids. The stagnation of fluid in the human body can only be resolved by lymphatic circulation. Since the circulation of bodily fluid occurs through lymph circulation, stagnation of bodily fluid means a disorder of lymphatic circulation. Relieving the stagnation of body fluids will eliminate swelling. Therefore, it can be directly confirmed that lymphatic circulation is improved from the improvement in edema according to the present invention. The following effects are of course physiologically predicted effects when lymph circulation is promoted.

2) The effect of improving inflammation and pain: Muscle pain is mainly caused by excessive use of muscles. Sensory nerves are loosely wound like a coil in the muscle fibers. Therefore, when the muscle fibers are excessively contracted or edema occurs due to accumulation of lactic acid, inflammatory substances, etc., and the muscle fibers become thicker than a certain degree, the sensory nerves are physically pulled tight, resulting in pain. Having such a structure is to prevent damage to muscles, ligaments, joints, and bones that are likely to occur due to excessive contraction or swelling of the muscles. Clinically, there are two cases in which muscle pain is caused, when there is no pain when not moving or when moving lightly, but when pain comes when exercise is strengthened, and when pain is caused even when not moving. There is no pain when not moving or moving lightly, but when the pain comes when exercising intensely, there is a slight swelling of the muscle fibers. When you do not move or move lightly, the muscles are relaxed or contracted properly and the sensory nerve fibers are not pulled tightly, so there is no pain. However, when the muscle contraction occurs by strong exercise, the muscle fiber becomes thick due to edema and the muscle fiber becomes thick due to the contraction, so that the sensory nerve fibers of the muscle fiber are pulled tightly, causing pain. In the case where pain is caused even when not moving, the sensory nerve fibers surrounding the muscle fibers are pulled tightly, causing pain even if there is no contraction of the muscle due to severe swelling of the muscle fibers. In both cases, if the swelling of the muscle fibers is rapidly improved by promoting lymph circulation according to the present invention, pain is reduced and the inflammatory substance that caused the edema is also removed, thereby improving the inflammatory state. Tendon pain is also mainly caused by excessive muscle use. Sensory nerve fibers are loosely attached to the tendon like a spider web. Therefore, when swelling occurs in the tendon due to the accumulation of lactic acid or inflammatory substances, or when the tendon is stretched more than necessary due to excessive contraction of the muscle fiber, the sensory nerves are physically pulled tight, resulting in pain. This structure is to prevent damage to muscles, tendons, ligaments, joints, bones, etc. that are likely to occur due to excessive contraction of the muscles. Clinically, when pain is caused in the tendon, there is no pain when it is not moving or when moving lightly, but when severe exercise causes pain, and when it is not moving, pain is caused. If you do not move or move lightly, there is no pain, but if you do severe exercise, pain comes in, there is a slight swelling of the tendon. There is no pain because the sensory nerve fibers attached to the tendon are not pulled tightly at the level of motionless or light movement. However, if more muscle contraction occurs due to severe exercise, the tendon becomes thick due to swelling and at the same time, the tendon is stretched due to the contraction of the muscle fiber, so that the sensory nerve fiber of the muscle fiber is pulled tight, causing pain. In the case where pain is caused even when not moving, pain is caused by the tension of the sensory nerve fibers wrapped around the tendon even if there is no contraction of the muscle due to severe swelling of the tendon. In both cases, when the swelling of the tendon is improved by promoting lymph circulation according to the present invention, pain is reduced, and at the same time, inflammatory substances that cause edema are also reduced, thereby reducing inflammation. Lymphatic circulation helps to circulate body fluids to improve pain in muscles and tendons. This situation can occur not only in the muscles and tendons, but also in other areas of inflammation.

3) Cell environment improvement effect: It promotes lymph circulation, improves the environment around the cells of the body tissues and helps cells to restore normal metabolism. Lymphatic circulation first aids blood circulation in the cardiovascular system and promotes the supply of nutrients, moisture, oxygen, hormones, etc., which are necessary substances for cells, and at the same time, it quickly removes waste products, toxic substances, and inflammatory substances accumulated in tissues or cells. To create an optimal environment in which children can normally operate. This is an immediate effect by allowing cells to efficiently utilize nutrients and oxygen in the body.

4) Blood bruise removal effect: Blood bruises are caused by rupture of blood vessels due to bruises or various causes, causing blood to come out of the blood vessels to form bruises under the skin, and appear red or dark red due to red blood cells in the blood. At this time, the red blood cells contained in the bruise cannot pass through the capillary walls due to their high molecular weight, so they must be decomposed on the spot or removed by lymphatic circulation. The promotion of lymphatic circulation according to the present invention promotes the movement of substances having a large molecular weight that cannot pass through the capillary walls, thereby rapidly removing bruises.

5) Body temperature regulation effect: The promotion of lymphatic circulation according to the present invention promotes the circulation of body heat through the circulation of body fluids. Lymphatic circulation promotes the transmission and distribution of body heat and improves the metabolism of cells to normalize a locally low body temperature, and when the body temperature is high, it helps to dissipate body heat and lowers the body temperature to normalize.

6) Effect of promoting drug delivery: When taking a drug prescribed for a specific disease or administering an injection, when promoting lymphatic circulation according to the present invention, the drug moves quickly and reliably to the lesion so that the administered drug effectively acts on the lesion. do.

These various effects can only be explained by the effect of promoting lymphatic circulation, both anatomically and pathologically. Therefore, it is possible to confirm the lymphatic circulation promoting effect of the present invention by confirming these individual effects.

As such, the functional food composition according to the present invention can be widely applied to improve various pathological symptoms.

1 is a photograph of the product contents of the functional food composition according to the present invention.
Figure 2 is a photograph of the product packaging of the functional food composition according to the present invention.
3 is a dissected photograph of a rat used to measure the change in lymph fluid flow rate of the present functional food composition.
Figure 4 is a chart showing the measurement result of the lymph fluid flow rate change of the functional food composition according to the present invention.
5 to 8 are diagrams showing the results of the edema improvement test of the functional food composition according to the present invention.
9 and 10 are diagrams showing pain improvement test results of the functional food composition according to the present invention.
11 is a chart showing the results of measuring the viscosity of the lymph fluid of the functional food composition according to the present invention.

The functional food composition for promoting lymphatic circulation according to the present invention includes cheonggung, maple syrup, crust, tabasheer, haedongpi and uiyiin in powder, liquid or extract form.

Functional food composition for promoting lymphatic circulation according to the present invention is composed of the following compositional components and composition ratio.

i) 20-50% by weight of cnidium known to be good for blood circulation (based on total weight, the same as below);

ii) 20-50% by weight of maple syrup, which has a sweet taste and is used as a sugar substitute or sweetener, but not known about its pharmacological action;

iii) 10 to 30% by weight of the crust known to cause stagnation to spin

iv) 10 to 30% by weight of tabasheer known to reduce heat and sputum,

v) 5-25% by weight of haedongpi, known to pass the meridians well;

vi) 1-20% by weight, which is known to be effective in edema.

If less than 20% by weight of cnidium is added, the effect of promoting lymphatic circulation is insignificant, and when more than 50% by weight of cnidium is added, the increase in the effect of promoting lymphatic circulation by the cnidium does not reach an increase in the amount of cnidium added.

If less than 20% by weight of maple syrup is added, the effect of promoting lymphatic circulation by maple syrup is insignificant, and if more than 50% by weight of maple syrup is added, the effect of promoting lymphatic circulation by maple syrup is increased. Does not reach

When the crust is added in less than 10% by weight, the effect of promoting lymphatic circulation by the crust is insignificant, and when the crust is added in more than 30% by weight, the increase in the effect of promoting lymph circulation by the crust does not reach the increase in the amount of crust added.

If tabasheer is added less than 10% by weight, the effect of promoting lymphatic circulation by tabasheer is insignificant, and if tabasheer is added in excess of 30% by weight, the increase in the effect of promoting lymphatic circulation by tabasheer does not reach an increase in the amount of tabasheer added.

If less than 5% by weight of thawed blood is added, the effect of promoting lymphatic circulation by the thawed skin is insignificant, and if more than 25% by weight of thawed blood is added, the increase in the effect of promoting lymph circulation due to the thawed skin does not reach the increase in the amount of added thawed blood.

If less than 1% by weight of Ui-in is added, the effect of promoting lymphatic circulation by Ui-in is insignificant, and when more than 20% by weight of Ui-in is added, the increase in the effect of promoting lymphatic circulation by Ui-in does not reach the increase of the amount of Uui-in.

The functional food composition according to the present invention may be made into a pill, granule, or powder for oral administration by mixing maple syrup and tabasheer in a mixture powder of cnidium, crust, haedongpi and uiyiin.

Functional food composition according to the present invention is a mixture of water, ethanol, or a liquid or powder extract extracted with water and ethanol from the mixed powder of cheonggung, crust, haedongpi and uiyiin, maple syrup, tabasheer, and internal medicine pills, granules, It can be made as a powder or liquid.

The functional food composition according to the present invention includes as an active ingredient an extract extracted with water, ethanol, or water and ethanol by mixing cinnamon, maple syrup, crust, tabasheer, haedongpi, and uiyiin at a certain ratio, as an active ingredient, pills, granules, It can be made as a powder or liquid.

Cheongung is a dried rootstock of a parsley family, such as Gunggung or Sangungi. The taste is spicy and the nature is warm.

Maple syrup is concentrated by collecting the sap of Canadian maple trees and is widely used as a substitute for sugar.

The crust is a mature fruit of a light-tangerine tree, which is known to cause stagnant energy to turn and to improve indigestion.

Tabasheer, It is a collection of bamboo sap that is evaporated by strong heat in the process of making charcoal out of bamboo.

Haedongpi is dried bark of the Ogalpiaceae family.

Uii-in is the dried sprouting seeds of the rice family plant, Yulmu. Yulmu is planted in various places in Korea. After the seeds are ripe in the fall, cut the outposts and pat them to dry, shake off the seeds, and peel the seeds.

Among them, tabasheer, haedongpi, and maple syrup have the most direct and powerful effect on promoting lymphatic circulation, regardless of their component ratio. Therefore, if any one component of tabasheer, thawed skin, and maple syrup is omitted, the effect of promoting lymph circulation is remarkably lowered. Cheongung, Jigak, and Uiiin supplement and strengthen the lymphatic circulation promoting effects of tabasheer, haedongpi, and maple syrup. Functional food composition according to the present invention exhibits a variety of effects such as improving edema, improving inflammation and pain, removing bruises, regulating body temperature, improving cellular environment, and promoting drug delivery. Because it does.

Since the promotion of lymphatic circulation creates an optimal environment for the metabolic activity of cells, the functional food composition according to the present invention can be used for almost all symptoms arising from an improper cell metabolic environment. In a narrow range, it can be used not only to improve edema, improve pain, improve inflammation, control body temperature, remove bruises, and promote drug delivery, but if expanded widely, it can be used for all symptoms that may be caused by lack of exercise, which is the cause of lymphatic circulation. have. In addition, the functional food composition according to the present invention can be used in almost all fields for preventing diseases, improving symptoms of diseases, and treating diseases.

It does not cause problems even if it is mixed with drugs or functional foods with other functions, but rather helps the absorption and delivery of active ingredients and improves the cellular environment of the site causing symptoms, thus increasing the efficacy of the active ingredients. For example, when the functional food composition according to the present invention is taken together with vitamin C or a fat-soluble blood circulation agent, the effect of reliably increasing their effect is obtained.

<Example 1: C4U-1>

For clinical trials and drug efficacy of the functional food composition according to the present invention, a functional food composition pill for internal use was prepared several times in the following amount.

Make a mixed powder of 2,700g of Chungoong powder, 1,400g of crust powder, 1,100g of haedongpi powder, 800g of Uuiin powder, and add 2,600g of maple syrup and 1,400g of tabasheer to make about 10,000g in a small ring with a diameter of 3-5mm. And packaged them in units of 4.8g.

<Example 2: C4U-2>

About 1,000 g of syrup was made by mixing 220 g of thawed blood extract, 500 g of maple syrup, and 280 g of tabasheer, and these were packaged in units of 4.8 g.

<Example 3: C4U-3>

Chungoong extract 540g, crust extract 300g, and Uiiin extract 160g were mixed to make about 1,000g of syrup, and these were packaged in units of 4.8g.

[Test for evaluating the change in the flow rate of lymphatic fluid by collecting lymphatic fluid from the mesenteric lymphatic vessels of mice]

1) Purpose of the experiment: To evaluate the increase in lymph flow rate of C4U-1, C4U-2, and C4U-3 samples, we established an experimental method for collecting lymph from the mesenteric lymphatic vessels of rats, and the effect of each sample on the lymphatic flow rate and The purpose of this study was to evaluate the effect of promoting lymphatic circulation.

2) Experiment method

(1) Animal treatment and sample administration

To check lymphatic vessels, 1.5 ml of peanut oil is administered orally to mice (Male, Sprague-Dawley Rat, weight 240 250g) that have been fasted for 24 hours. After 30 minutes of administration of Peanut oil, C4U-1 was administered orally by taking an amount equivalent to 1,666 mg to form a powder, and then dispersing it in a small amount of purified water. C4U-2 and C4U-3 were administered orally by taking 3,636 mg accurately and dividing it into two. As a control, purified water in the same amount as C4U-2 and C4U-3 was orally administered. After 30 minutes after administration of the sample, 1 ml of urethane was administered intraperitoneally to anesthetize the rat.

(2) Lymphatic fluid collection and lymphatic fluid flow measurement

As shown in Figure 3, the peritoneum of the anesthetized rat was incised 3-4 cm. At this time, the small intestine was slightly moved to the side of the abdominal cavity and then protected with gauze soaked in warm physiological saline. A fine tube made of polyethylene (0.5 mm inner diameter, 0.9 mm outer diameter) was inserted into a white and shiny lymphatic vessel (see FIG. 3) located vertically in the vena cava under the liver, and the lymph fluid was collected for 1 hour. After 1 hour, the microtube was removed, and the weight of the flow rate of the lymph fluid flowing into the microtube was measured with a microbalance.

3) Experiment result

The test results are shown graphically in FIG. 4. As can be seen with reference to Figure 4, The flow rate of lymph fluid collected for 1 hour without administration to rats was 1.1 mg by weight, and there was almost no change in the flow rate of lymph fluid even when measured after administration of purified water. However, after administration of C4U-1, C4U-2, and C4U-3, the flow rate increased significantly to 1.8 mg (63.6% increase), 1.6 mg (45.4% increase), and 1.3 mg (18.2% increase). It was found that samples 1, C4U-2, and C4U-3 can significantly increase the flow rate of lymph fluid. This change in flow rate was noticeable even during lymphatic collection. Through this, it can be seen that the circulation of lymph fluid is promoted when C4U-1, C4U-2, and C4U-3 are administered. In particular, it can be seen that the increase rate of lymphatic circulation promotion of C4U-2 is more than twice that of C4U-3, and the increase rate of lymphatic circulation promotion of C4U-1 is more than 3 times of that of C4U-3. Able to know. In other words, it was confirmed that the extracts of Haedongpi, Maple syrup, and tabasheer had a greater effect on promoting lymphatic circulation than the extracts of Cheongung, Jigak and Uiiin, and when they were all mixed, the effect of promoting lymphatic circulation was synergistic.

[Carrageenan-induced foot edema test using Sprague-Dawley rat]

1) Test purpose

The easiest way to check the effect of promoting lymphatic circulation is the edema test. This is because almost all conditions that cause edema involve stagnation of lymph fluid. Therefore, the lymphatic circulation effect can be confirmed as a method of confirming the improvement of edema.

2) Test substance

C4U-1, C4U-2, and C4U-3 were used as test materials, and Diclofenac Sodium was used as a positive control material. As excipient 1, sterile water for injection (Daehan sterile distilled water-source: Daehan Pharmaceutical Industries Co., Ltd.) was used, and as excipient 2, 0.9% saline (sterile physiological saline solution-source: Daehan Pharmaceutical Industries, Ltd.) was used.

3) Test method

(1) Test preparation

Specific pathogen-free (SPF) rat, Hsd:Sprague Dawley®™ SD®™ (producer and source: Coretech Co., Ltd. (181-21, Jinwi-ro, Jinwi-myeon, Pyeongtaek-si, Gyeonggi-do), male 6 weeks old, 30 males were used. Rats were chosen because they are widely used in various drug efficacy and toxicity tests, and abundant test data are accumulated, and it is easy to interpret and evaluate test results using these data. , During the administration and observation period (red), an individual identification card was attached to the breeding box, and an animal room usage record was attached to the entrance of the breeding room. 3 ℃, relative humidity 55±15%, ventilation frequency 10-20 times/hr, lighting time 12 hours (8 am to 8 pm off) and illuminance 150 to 300 Lux. During the breeding period, environmental conditions such as temperature/humidity, ventilation frequency and illuminance in the animal room were measured regularly. Rho 507) and freely ingested water, using a polycarbonate drinking bottle for water, and a wooden rug supplied from Coretech Co., Ltd. (406 Dongcheon-ri, Jinwi-myeon, Pyeongtaek-si, Gyeonggi-do). During the period of acclimatization and experimentation, no more than 5 animals/carriers were raised in a polycarbonate breeding box for rodents (W 235 x L 380 x H 175 mm) The breeding boxes, rugs, and water bottles were exchanged at least once a week. The weights of animals determined to be healthy were measured and grouped by a random method so that the average weight of each group was distributed as uniformly as possible according to the ranked weight.

(2) Composition of test group

The composition and dosage of the test group were determined as follows. Test substance 1 represents C4U-1, test substance 2 represents C4U-2, and test substance 4 represents C4U-3. As described above, the positive control material is Diclofenac Sodium.

(3) Preparation of test substance for administration

In the case of Carrageenan, after weighing the required amount, it was prepared by dissolving it at a concentration of 1% (10mg/mL) in sterile physiological saline as an excipient, and used as an edema inducing substance.

In the case of a test substance, at the request of the client, weigh the required amount according to the following information provided by the client, and add sterile water for injection, prepare it to a concentration of 400 mg (based on the main ingredient) / 10 mL, and then use it for the test. I did.

Test substance 1 (C4U-1)

Formulation: Pill

Prescription: 240 mg of active ingredient in 1000 mg of pills, 760 mg of excipient

Dosage: 400 mpk as the main ingredient (about 1666 mg as a powder)

Test substance 2 (C4U-2)

Formulation: Syrup

Prescription: 110 mg of active ingredient in 1000 mg of syrup, 890 mg of excipient

Dosage: 400 mpk as the main ingredient (about 3636 mg as a syrup)

Test substance 3 (C4U-3)

Formulation: Syrup

Prescription: 110 mg of active ingredient in 1000 mg of syrup, 890 mg of excipient

Dosage: 400 mpk as the main ingredient (approximately 3636 mg as a syrup)

In the case of a positive control substance, the required amount was taken, and then prepared to a concentration of 30 mg/10 mL by adding sterile water for injection, and used for the test.

(4) administration

In the case of the test substance, oral administration, which is the pre-clinical route, was selected. In the case of Carrageenan, an edema inducing substance, intradermal administration to the sole of the foot was selected. In the case of the test substance, a single dose was administered 2 hours before the administration of the trigger substance (Carrageenan), and the trigger substance was also administered once. In the case of the test substance and the positive control substance, it is calculated and administered as 10 mL/kg based on the body weight measured on the day of the test. In the case of Carrageenan, 50 μL was administered intradermally to the sole of the right hind limb. In the case of oral administration, animals were fixed by the cervical skin fixation method, and administered directly into the stomach using a sonde for oral administration. In the case of Carrageenan, 50 μL was administered intradermally to the sole of the right hind limb. Animals for administration of the test substance were fasted for 16 hours or more before administration of the test substance.

(5) Observation and inspection items

Before Carrageenan administration and 3 hours after injection, measure the volume of the hind limb with a leg edema meter.

It was measured using, and the edema inhibition (%) was calculated using the calculation formula presented below.

* The edema rate (%) and edema inhibition (%) of each test group were calculated.

Edema rate (%) = {(Vt-Vn)/Vn} X 100

Vt: Foot volume after a certain period of time after Carrageenan injection,

Vn: Foot volume before Carrageenan injection

Edema inhibition power (%) = (average edema rate of inducing control group. Edema rate of each individual) / (edema rate of inducing control group) x 100

4) Test result

The test results are shown graphically in FIGS. 5 to 8.

5 is a result of measuring the volume of the hind limb using a lower extremity edema meter before injection of the test substance and administration of Carrageenan. It can be seen that the volume is around 1 mL in all groups (G1-G6). 6 is a result of measuring the volume of the hind limb 3 hours after administration of Carrageenan to the G2-G6 group using a lower limb edema meter, and FIG. 7 is a difference between the measurement result of FIG. 6 and the measurement result of FIG. 5 (edema Increase volume). FIG. 8 is a table showing the results of calculating the edema rate using the measured value of FIG. 7 and calculating the edema suppressing power (%) using this edema rate.

As can be seen in FIG. 8, it can be seen that the edema inhibitory power of G4(C4U-1) is close to 17%, which is the edema inhibitory power of Diclofenac Sodium as a positive control material. It can be seen that the edema suppressing power of G5(C4U-2) was around 8%, and that of G6(C4U-3) was around 4%. It can be seen that C4U-1, in which the components of C4U-2 and C4U-3 are combined, exhibits a synergistic effect of improving edema. In addition, it is possible to confirm the lymph-promoting effect of the present invention through the effect of improving edema.

[PBQ-induced twist test using Sprague-Dawley rat]

1) Test purpose

Pain is the result of the accumulation of pain-causing substances in the muscles and back. When lymphatic circulation is promoted, the excretion of pain-causing substances is promoted. This test was conducted to evaluate the effect of treatment of test substances in a pain inducing model using Sprague Dawley rats. The lymphatic circulation effect can be checked as a way to check the pain improvement.

2) Test substance

C4U-1, C4U-2, and C4U-3 were used as test materials, and Diclofenac Sodium was used as a positive control material. As excipient 1, sterile water for injection (Daehan sterile distilled water-source: Daehan Pharmaceutical Industries Co., Ltd.) was used, and as excipient 2, 0.9% saline (sterile physiological saline solution-source: Daehan Pharmaceutical Industries, Ltd.) was used.

3) Test method

(1) Test preparation

Specific pathogen-free (SPF) rat, Hsd:Sprague Dawley®™ SD®™ (producer and source: Coretech Co., Ltd. (181-21, Jinwi-ro, Jinwi-myeon, Pyeongtaek-si, Gyeonggi-do), male 6 weeks old, 30 males were used. Rats were chosen because they are widely used in various drug efficacy and toxicity tests, and abundant test data are accumulated, and it is easy to interpret and evaluate test results using these data. , During the administration and observation period (red), an individual identification card was attached to the breeding box, and an animal room usage record was attached to the entrance of the breeding room. 3 ℃, relative humidity 55±15%, ventilation frequency 10-20 times/hr, lighting time 12 hours (8 am to 8 pm off) and illuminance 150 to 300 Lux. During the breeding period, environmental conditions such as temperature/humidity, ventilation frequency and illuminance in the animal room were measured regularly. Rho 507) and freely ingested water, using a polycarbonate drinking bottle for water, and a wooden rug supplied from Coretech Co., Ltd. (406 Dongcheon-ri, Jinwi-myeon, Pyeongtaek-si, Gyeonggi-do). During the period of acclimatization and experimentation, no more than 5 animals/carriers were raised in a polycarbonate breeding box for rodents (W 235 x L 380 x H 175 mm) The breeding boxes, rugs, and water bottles were exchanged at least once a week. The weights of animals determined to be healthy were measured and grouped by a random method so that the average weight of each group was distributed as uniformly as possible according to the ranked weight.

(2) Composition of test group

The composition and dosage of the test group were determined as follows. Test substance 1 represents C4U-1, test substance 2 represents C4U-2, and test substance 4 represents C4U-3. As described above, the positive control material is Diclofenac Sodium.

(3) Preparation of test substance for administration

In the case of PBQ, after weighing the required amount, use a concentration of 2 mg/10 mL in sterile physiological saline as an excipient.

It was prepared by dissolving and used as a pain-causing substance.

In the case of a test substance, at the request of the client, weigh the required amount according to the following information provided by the client, and add sterile water for injection, prepare it to a concentration of 400 mg (based on the main ingredient) / 10 mL, and then use it for the test. I did.

Test substance 1 (C4U-1)

Formulation: Pill

Prescription: 240 mg of active ingredient in 1000 mg of pills, 760 mg of excipient

Dosage: 400 mpk as the main ingredient (about 1666 mg as a powder)

Test substance 2 (C4U-2)

Formulation: Syrup

Prescription: 110 mg of active ingredient in 1000 mg of syrup, 890 mg of excipient

Dosage: 400 mpk as the main ingredient (about 3636 mg as a syrup)

Test substance 3 (C4U-3)

Formulation: Syrup

Prescription: 110 mg of active ingredient in 1000 mg of syrup, 890 mg of excipient

Dosage: 400 mpk as the main ingredient (approximately 3636 mg as a syrup)

In the case of a positive control substance, the required amount was taken, and then prepared to a concentration of 30 mg/10 mL by adding sterile water for injection, and used for the test.

(4) administration

In the case of PBQ, intraperitoneal administration was selected based on references. In the case of PBQ, based on the body weight measured on the day of the test, it was calculated and administered as 10 mL/kg. In the case of the test substance, the triggering substance (PBQ) was administered once 2 hours before administration, and the triggering substance was also administered once. In the case of the test substance and the positive control substance, it is calculated and administered as 10 mL/kg based on the body weight measured on the day of the test. In the case of oral administration, animals were fixed by the cervical skin fixation method, and administered directly into the stomach using a sonde for oral administration. In the case of PBQ, animals are fixed with the cervical skin fixation method and administered intraperitoneally using a syringe equipped with a 26 gauge injection needle. Animals for administration of the test substance were fasted for 16 hours or more before administration of the test substance.

(5) Observation and inspection items

From 10 minutes after PBQ administration, the total number of twists was measured for 30 minutes for each individual, and the pain suppression power (%) was calculated using the calculation formula presented below.

Pain suppression power (%) = (number of twists in the trigger control group. Number of twists in the drug administration group) / (number of twists in the trigger control group) x 100

4) Test result

The test results are shown graphically in FIGS. 9 and 10.

9 is a table showing the results of measuring the total number of twists for 30 minutes for each individual from 10 minutes after administration of PBQ, and FIG. 10 is a chart showing the pain suppression power (%) calculated using the results of FIG. 9.

As can be seen in FIG. 10, it can be seen that the edema inhibitory power of G4 (C4U-1) is close to 42%, which is the edema inhibitory power of Diclofenac Sodium as a positive control material. It can be seen that G5 (C4U-2) has an edema suppressing power of around 39%, and G6 (C4U-3) has an edema suppressing power of around 8%. It can be seen that the component of C4U-2 particularly exerts a great effect on the pain suppressing power, and when combined with the C4U-3 component, as the result of C4U-1, it exhibits a greater pain suppression effect.

Through this pain suppression effect, the lymph promoting effect of the present invention can be confirmed.

[Viscosity Evaluation of Lymphatic Fluid Collected from Mesenteric Lymphatic Vessels in Rats]

1) When C4U-1, C4U-2, and C4U-3 were administered, it was expected that there would be a change in the viscosity of the lymph fluid, and the viscosity of the lymph fluid collected after administration of the sample to the rat was evaluated.

2. Experiment method

1) Animal treatment and sample administration

(1) To 40 rats (Sprague-Dawley rat, male, weight 190-200g) that had been fasted for 24 hours, TPA (12-O-tetradecanoylphorbol-13-acetate) was used at four points of the ears and lower extremities of the rat. Caused skin irritation.

(2) 6 hours after the induction of inflammation, 40 rats were divided into 4 groups of A, B, C, D by 10, and then powdered for groups B, C, and D excluding group A (control group). C4U-1, C4U-2, C4U-3 1,666 mg each was dispersed in purified water (4.5 ml) and administered orally.

(4) After 30 minutes of administration of C4U-1, C4U-2, C4U-3 as above, anesthetize the rat with chloroform, and then additionally administer 1 ml of urethane intraperitoneally to maintain the anesthesia of the rat during the experiment. I did.

2) Lymphatic collection

(1) After anesthetizing the rat, the peritoneum of the rat was incised 3-4 cm. After that, the small intestine was placed outside the abdominal cavity and covered with gauze soaked in warm physiological saline to prevent drying.

(2) Insert a polyethylene microtube (ID 0.5 mm, OD 0.9 mm) into a lymphatic vessel located vertically in the vena cava under the liver, and extract the lymph fluid leaking out of the tube in each group A, B, C, D. I collected not much.

3) Measurement of the viscosity of lymph fluid

(1) 2,000 μl of lymph fluid was taken for each group, and a rheometer (model name: HAAKE RheoStress 1, Thermo Fisher Scientific Co. Ltd.) equipped with a 35 mm diameter parallel plate (P TiL 30, Thermo Fisher Scientific Co. Ltd., USA). , USA) was used to measure the viscosity of the lymph fluid. At this time, the gap between the two plates was set to 1.0 mm, and the temperature of the lower plate was set to 20℃.

(3) After putting about 2,000 μl of sample between the two plates, the sample was stabilized for 5 minutes.

(4) The strain value of the sample was measured while applying a shear stress of 0.1 Pa for 500 seconds.

delete

(5) After the measurement was completed, the zero shear viscosity was calculated.

3. Experiment result

As shown in FIG. 11, in the case of the control group (group A), the zero shear viscosity was measured as an average of 2.21 mPa·s, and C4U-1 (group B), C4U-1 (group C), and C4U-3 (group D. ), the zero shear viscosity averaged 1.78 mPa·s (about 19.5% decrease in viscosity), 1.85 mPa·s (about 16.3% decrease in viscosity), and 1.93 mPa·s (about 12.7% decrease in viscosity). ) Was measured.

[Measurement of drug delivery promotion effect]

1) Purpose of the test

Promoting lymph circulation can lead to promotion of drug delivery. Therefore, if the drug delivery effect as well as the edema improvement effect and pain improvement effect are confirmed, the lymphatic circulation effect can be confirmed through these.

2) Test method

One point of the head of 10 hairless mice was infected with Staphylococcus aureus, Candida albicans, Microsporium canis, and yeast to cause erythema and inflammation.

In the 5 hairless mice selected as the test group, 0.5 g of C4U-1 was ground in powder form immediately after the occurrence of erythema and inflammation, mixed with hot water, and continuously administered orally every 6 hours, while 30 mg of vancomycin was added to the hairless mice every 12 hours. Was injected into. In 5 hairless mice selected as a control group, 30 mg of vancomycin was injected into the lower limbs of hairless mice at 12 hour intervals immediately after the occurrence of erythema and inflammation. For the test group and the control group, skin conditions were observed for 84 hours every 12 hours after the initial injection.

3) test result

As a result of the above experiment, in the case of the mice in the test group, it was observed that the inflammation subsided and the erythema was alleviated from the lapse of 12 hours in 4 mice. In the remaining one mouse, it was observed that the inflammation subsided and the erythema was relieved after 24 hours. After 72 hours, erythema and inflammation were no longer observed in 3 mice. After 84 long elapses, erythema and inflammation were no longer observed in all mice. In addition, no toxicity was observed in hairless mice during the test.

On the other hand, in the case of the mice of the control group, erythema tended to alleviate after 48 hours in 2 mice, and the remaining 3 mice showed a tendency to alleviate erythema after 72 hours. It was observed that the mice of the control group still had inflammation after 84 hours.

Through the test results, the drug delivery promoting effect of the present invention can be confirmed, and the lymphatic circulation effect of the present invention can be confirmed through the drug delivery promoting effect.

[Clinical test]

Hereinafter, unless otherwise described, the functional food composition according to the present invention is C4U-1 according to Example 1.

<Measurement of edema improvement effect>

Edema refers to a condition in which fluid, such as lymph fluid or tissue exudate, is accumulated and excessively present in the tissue due to lymphatic circulation disorder. When swelling occurs in the skin and soft tissues, it swells clinically and has a loose sensation, and when pressed, the skin temporarily dents. If the lymphatic fluid circulates smoothly, these symptoms disappear. Therefore, improvement of edema means improvement of lymphatic circulation. If edema improvement is confirmed, it can be concluded that there is an improvement in lymphatic circulation.

50 female patients who are 50 years of age or older with edema of the lower extremities were selected, and these patients were asked to take the functional food composition according to the present invention twice a day, 2 packets at a time, and after taking 7 consecutive days, Each patient was asked to measure i) change in weight (using a weight scale), ii) change in circumference length of the area with the most edema among thighs, calves, and insteps (using a plastic tape measure), and iii) edema status (visual observation). The results are shown in Table 3 below.

From the measurement results below, it was confirmed that the circumference length of the most severe part of the lower extremity edema decreased in 45 (90%) of 50 patients, and that weight decreased in 40 (80%) patients. And, it was confirmed that the edema condition was alleviated in 46 patients (92%). It was confirmed that the circumference of the edema area decreased in the range of 1.0% to 10.0% of the circumference before administration. It was confirmed that the body weight decreased in the range of 0.5% to 4.0% of the body weight before administration. Body fluid (lymphatic or tissue exudate) stagnated in the lower extremity tissue was discharged into the stool through urine or bile, resulting in a change in body weight. Improvements in edema are usually accompanied by significant weight loss.

From these results, it can be confirmed that the edema of the present invention is improved, that is, lymph circulation is improved.

Measurement item

Number of patients (persons)
Patient rate


Changes in the length of the circumference of the thigh, calves, or instep where the swelling is most severe
The circumference of the edema area has decreased.

45
90%
There is no change in the circumference of the edema area

4
8%

The circumference of the edema area has increased.

One
2%



Weight change
I lost weight.

40
80%
No change in weight

9
18%
Gained weight

One
2%



Edema condition
The edema condition has been alleviated.

46
92%
No change in edema condition

4
8%
The edema condition worsened.

0
0%

<Measurement of pain relief effect>

As described above, the promotion of lymphatic circulation relieves pain through rapid discharge of pain-causing substances anatomically or pathologically. Therefore, of the present invention Through checking the pain relief effect, the lymphatic circulation promoting effect of the present invention can be confirmed.

10 patients with low back pain who feel back pain even without movement were selected, and 5 patients (M1-M5) were asked to take C4U-1 once a day for 28 days, 3 times a day, and the remaining 5 patients (m1-m5) had to take C4U-1. C4U-3 was administered three times a day, one bag at a time for 28 days, and the effect was measured.

After 28 days of taking, 3 points were given if no pain was felt even when moving, 1 point was given if pain was not felt when not moving, but 1 point was felt when pain was felt when moving, and 0 points were given if pain was still felt even when not moving.

The measurement results are shown in Table 4 below.

From the measurement results below, it can be seen that the degree of pain improvement of patients taking C4U-1 is superior to that of patients taking pills according to C4U-3.

Patients taking C4U-1

Patients taking C4U-3

M1

M2
M3
M4
M5

m1
m2
m3
m4
m5
3

3
3
2
3
2
0
2
2
0

Through this, the effect of promoting lymphatic circulation of the present invention can be confirmed.

<Measurement of bruise removal effect 1>

The disappearance of bruises is directly related to lymphatic circulation either anatomically or pathologically. Therefore, it can be said that the rapid disappearance of the bruise means the promotion of lymphatic circulation.

For 10 female patients with bruises due to rupture of blood vessels around the eyes due to the maesun procedure, 5 patients (M1 to M5) were given the functional food composition according to the present invention to be continuously taken 3 times a day, 2 packets at a time, and the remaining 5 The people (m1~m5) were given an egg massage 6 times a day for 5 minutes or more around the blood mung. The time required for the bruising to no longer be observed with the naked eye was measured.

The measurement results are shown in Table 5 below.

Patients taking C4U-1

Egg massage patient
M1

M2
M3
M4
M5
m1
m2
m3
m4
m5
15th

17th
13th
18th
11th
55 days
45 days
60 days
75 days
70 days

From this measurement result, it was confirmed that the bruising removal rate of the patient taking the example of the present invention was three times faster than the bruising removal rate of the patient, although the egg massage was performed.

<Measurement of bruise removal effect 2>

For 10 male patients whose eyes were bruised to a similar degree due to a bruise, 5 patients (M1 to M5) were allowed to continuously take the pills according to the present invention 3 times a day at a time of 2 capsules at a time, and the remaining 5 patients (m1 ~m5) were subjected to egg massage 6 times a day for 5 minutes or more around the blood meng. The time required for the bruising to no longer be observed with the naked eye was measured.

The measurement results are shown in Table 6 below.

Patients taking C4U-1

Egg massage patient
M1

M2
M3
M4
M5
m1
m2
m3
m4
m5
5 days

4 days
7 days
3 days
6 days
14 days
18th
20 days
15th
16th

From the measurement results of bruising patients due to bruises, it was confirmed that the bruising removal rate of the patient taking the embodiment of the present invention was 2 to 3 times faster than the bruising removal rate of the patient, although egg massage was performed.

Through this, the effect of promoting lymphatic circulation of the present invention can be confirmed.

<Measurement of Minimum Masking Level (MML) for Tinnitus Patients>

5 relatively severe male tinnitus patients and 5 female tinnitus patients were selected, and the functional food composition according to Example 1 in the present invention was given a single packet at a time with tid for 1 month, and the minimum shielding was performed before and after 1 month of initiation. The threshold (MML) was measured. The minimum shielding threshold (MML) refers to the minimum value at which the tinnitus is not heard by increasing the noise including the tinnitus frequency by 1dB in the ear where the tinnitus is heard. In general, a decrease in the minimum shielding threshold is interpreted as an improvement in tinnitus. The decrease in the minimum shielding threshold means that the tinnitus intensity decreases.

First, the selected tinnitus patients were acquired through a pitch matching test. While increasing the narrow-band noise frequency including the tinnitus frequency found in the tinnitus frequency test by 1dB, the shielding sound is heard to the ear with the tinnitus, and the minimum intensity value (minimum shielding threshold) of the shielded sound in which the tinnitus is not heard is obtained, and the Sensation Level ( dB SL) (difference from the listening threshold). The measurement results are shown in Table 7 below. The results below show that after taking the functional food composition according to the present invention in tinnitus patients, the minimum shielding threshold decreased in 9 out of 10 patients. This means that the degree of tinnitus was alleviated in 9 out of 10 patients. The magnitude of improvement is 1dBSL-4dBSL, although there is individual deviation, it shows significance for tinnitus improvement overall.

patient

M1
M2
M3

M4
M5
W1
W2
W3
W4
W5
tinnitus
frequency
(Hz)


4,000

3,000

4,000

4,000

5,000

3,000

4,000

4,000

4,000

5,000
Before taking
MML
(dBSL)


5

7

8

8

6

8

8

7

7

7
After taking 1 month
MML
(dBSL)

5


4

4

6

5

6

5

3

4

4

There are various causes of tinnitus, but it is thought that tinnitus is eventually caused by affecting the endolymph fluid in the cochlea. If the amount of lymph fluid in the cochlea is too high or low for any reason, changes in the concentration, pressure or temperature of the lamp fluid, changes in the composition of the lymph fluid, or problems with the circulation of the lamp fluid, This ideally stimulates the hair cells, and the stimulation is seen as being transmitted to the brain through the auditory nerve. The functional food composition according to the present invention basically improves the environment around cells by smoothing lymph circulation in the body, helps to restore normal metabolism of cells, and creates an environment so that cells in the body can function normally. give. Directly, it promotes the circulation of lymph fluid in the cochlea, restores insufficient or excessive lymph fluid in the cochlea to an appropriate level, and quickly discharges impurities or wastes mixed in the lymph fluid in the cochlea. As a result, the amount, composition and circulation of lymph fluid in the cochlea are quickly restored to a normal state, and the environment and function of the auditory cells (hair cells) are restored to normal, thereby rapidly improving tinnitus symptoms. In addition, by promoting the circulation of lymphatic fluid, the movement of substances is promoted, which facilitates the supply of nutrients, oxygen, and moisture necessary for hair cells, improves nutritional imbalance, and facilitates metabolism by helping the movement of cellular metabolites. So that the hair cells do not overreact in response to changes in the lymphatic fluid.

<Clinical example for individual patients>

The following clinical trial results are, of course, physiologically predicted results when lymph circulation is promoted, and are confirmed through clinical practice.

1) Clinical trial 1-Improvement of edema (43 years old, female)

After surgery for thyroid cancer, the patient continued to take the thyroid drug Synthroid for a long period of time and continued to have symptoms of swelling of the whole body as well as the face and fingers every morning due to weak poisoning. It was observed with the naked eye that facial swelling disappeared after 7 days and the swelling of the whole body disappeared after 5 days while starting to eat the functional food composition according to the present invention as b.i.d. It was confirmed that the weight was also reduced by 2.0 kg. The patient himself stated that his body became lighter, his face became smaller, and his skin was improved. Treatment of edema generally limits salt intake or administer diuretics, but does not produce satisfactory effects. According to the present invention, it was confirmed that a very rapid edema improvement effect appeared. Since edema is caused by stagnation of body fluids, and stagnation of fluid is caused by lymphatic circulation disorders, the improvement in edema means that lymph circulation is improved.

2) Clinical trial 2-Improvement of edema (54 years old, female)

This patient was a 54-year-old female pharmacist with frequent allergies and swelling. Due to degenerative arthritis, there was always swelling in the knee. I was bitten by a mosquito a few days ago, but I left it. After two days, my wrist became swollen and itchy. When I applied the steroid ointment, it spread largely, swelling and fever. Two packets of the functional food composition according to the present invention were taken, and two packets were again taken after 5 hours. The next morning, little swelling of the wrist was observed. After that, 1 packet of b.i.d was taken for 5 more days, and the swelling of the knee was no longer observed. Since edema is caused by stagnation of body fluids, and stagnation of fluid is caused by lymphatic circulation disorders, the improvement in edema means that lymph circulation is improved.

3) Clinical trial 3-Improvement of edema (40 years old, female)

A 40-year-old female patient who was overweight and complained of frequent swelling of the insteps and soles of the feet and pain in the back neck and shoulders. The functional food composition according to the present invention was taken for 5 days in t.i.d by 2 packets each. Five days after taking the drug, the swelling of the back and soles completely disappeared, and he stated that he felt a little less weight. It was judged that a large amount of systemic waste products were discharged by promoting lymphatic circulation.

4) Clinical trial 4-Improvement of edema (72 years old, female)

It was a female patient with signs of cracked skin due to swelling of the lower extremities for more than 10 years. His skin was sensitive, so he felt pain even when he touched his hand slightly. Entelon Tab (150mg), marketed as a circulatory system drug, was taken for several years, but there was no improvement. This patient was allowed to take 3 packets of the functional food composition according to the present invention in t.i.d for 20 days. After 20 days, it was visually confirmed that the swelling was reduced to the extent that wrinkles appeared on the calf skin.

5) Clinical trial 5-Pain improvement (48 years old, female)

She is a female pharmacist and complained that her wrist pain was so severe that she couldn't lift a box of 500g medicine at a pharmacy. One packet of the functional food composition according to the present invention was taken for 3 days as t.i.d. It was stated that wrist pain was relieved from the first dose of 1 packet, and that wrist pain had completely disappeared on the third day. Since the promotion of lymphatic circulation promotes the discharge of pain-causing substances, it is possible to confirm the lymphatic circulation effect of the present invention.

6) Clinical trial 6-Pain improvement (50 years old, female)

It was a strange patient who had been doing business in the market for 30 years. This patient had acupuncture and painkillers for a long time due to severe pain in the ankle and heel. After taking the functional food composition according to the present invention for 5 days in t.i.d by 2 packets each, it was made to take more as b.i.d by 1 packet for 10 days. After about 2 weeks, the patient stated that he no longer felt pain in the ankle and heel. Since the promotion of lymphatic circulation promotes the discharge of pain-causing substances, it is possible to confirm the lymphatic circulation effect of the present invention.

7) Clinical Trial 7-Pain improvement (80 years old, female)

It was a patient with a grandmother who had back surgery and knee artificial surgery and carried a cane because her whole body was sore. After taking the functional food composition according to the present invention for 10 days in t.i.d by 2 packets each, 1 packet by b.i.d for 3 months from 10 days later was further taken. This patient stated that the pain in the lower back and knee had been relieved after about 2 days, and that all the pain in the whole body had disappeared after 3 months. Since the promotion of lymphatic circulation promotes the discharge of pain-causing substances, it is possible to confirm the lymphatic circulation effect of the present invention.

8) Clinical trial 8-Pain improvement (61 years old, female)

A woman in her 60s, she was a caregiver from China. She said she had severe back pain since she was young. The patient explained that the pain in the lower back became more severe as he was taking care of it, but he also pulled the buttocks and thighs and explained that it hurts. When the functional food composition according to the present invention was taken for 10 days in t.i.d, one packet at a time, the patient stated that the pain was greatly relieved after 10 days. After 10 days, they were asked to take one more b.i.d at a time for 3 months. After 3 months, it was stated that not only back pain but also thigh pain disappeared. Since the promotion of lymphatic circulation promotes the discharge of pain-causing substances, it is possible to confirm the lymphatic circulation effect of the present invention.

9) Clinical trial 9-Pain improvement (31 years old, female)

This patient was a 31-year-old female track and field athlete. This patient suffered an injury to the Achilles tendon during training. This patient had undergone hospital treatment for a considerable period of time, but there was no improvement in symptoms at all. Not only was this patient unable to run, but he also struggled to walk. In addition, the entire Achilles tendon is swollen, so even if you touch it slightly with your fingertips, you are in a state of complaining of severe pain that makes you yell that it hurts. Upon closer examination, the entire Achilles tendon was in a state of severe swelling due to inflammation and a state of severe pain due to severe swelling.

When the functional food composition according to the present invention was intensively taken for 3 days with 3 packets of t.i.d at a time, the pain was improved to the extent that there was no pain in touching and massaging the Achilles tendon, and there was no difficulty in walking. Thereafter, after the functional food composition according to the present invention was taken for 7 days with t.i.d at a time of 2 packets, it was possible to jog through morning exercise. After 7 days of taking the functional food composition according to the present invention as tid by 2 packets each, it was possible to exercise by increasing the exercise intensity by 90%, and there was almost no pain in the Achilles tendon. Strong lymph circulation promotion in athletes Achilles This is a good example of what helped recover a tendon injury. Since the promotion of lymphatic circulation promotes the discharge of pain-causing substances, it is possible to confirm the lymphatic circulation effect of the present invention.

10) Clinical trial 10-Improvement of inflammation (42 years old, male)

This patient The Achilles tendon was broken in an accident while riding a motorcycle before, and the surgical site became inflamed after the joint surgery. Since then, the inflammation worsened almost every year, and although dozens of reoperations and treatments were performed, the inflammation did not heal clearly. Seoul National University Hospital confirmed the infection of superbacteria and virus in the wound, and said that it is no longer possible to cure the wound with surgery and drug therapy. Also, if the inflammation worsens, there is a risk of systemic infection, so amputation of the ankle was recommended. In this state, when the functional food composition according to the present invention was intensively taken for 5 days with 4 packets of tid at a time, blood pus appeared at first, and immediately after a large amount of dark black blood came out, the size of the inflammation was greatly reduced. Thereafter, when the functional food composition according to the present invention was taken for 7 days as tid at a time of 3 packets, the inflammation subsided cleanly and there was no discomfort due to the affected area. Of course, there was no ankle amputation, and when retested at the hospital, the superbacterial virus was no longer identified. In this case, it was thought that the functional food composition according to the present invention promotes circulatory discharge of inflammatory substances, strengthens immune functions, and helps the movement of immune cells, thereby removing super bacteria and viruses from the inflammatory site, thereby eliminating inflammation.

<Clinical Example 11> Promoting skin cell regeneration (45 years old, male)

The patient of Clinical Example 1 shown in Table 7 below was a patient who was damaged to the dermal layer due to an accident where a hand was caught in the belt, and it was diagnosed as a situation in which regeneration was impossible because it was a very large wound over 5 cm in all directions in the hospital, and skin transplantation was essential.

Photo a) Before treatment Picture b) After 2 months of patch replacement twice a week

Photo c) After 4 months of patch replacement twice a week Photo d) After 6 months of patch replacement twice a week

As can be seen from the photos in Table 8, when no treatment is performed in this patient or patients with similarly affected areas, or when only massage management is applied, not only damage to skin tissues but also internals such as muscles, blood vessels and nerves It can cause serious damage to the tissues. In addition, such severe burns are accompanied by complex pathological and physiological changes. The patch used in Example 1 was attached and the pill was taken as t.i.d at a time of 2 packets at a time. As can be seen in photo b of Table 8, recovery of the dermal layer was observed in most of the burned areas after 2 months of treatment. In addition, as can be seen in photo c of Table 8, it was observed that a significant portion of the epidermal layer was recovered in most of the burned areas after 4 months of treatment. In addition, as can be seen in photo d of Table 8, it was observed that all of the epidermal layer was recovered in most of the burned areas after 6 months of treatment. In addition, keloids were also observed finely.

The task of improving scars by reducing already formed keloids takes a long time and several efforts and is a difficult task, but reducing the production of keloids before the formation of keloids to prevent scarring is simpler than improving already formed keloids. It's an easy task. In order to have a keloid-like wound on the skin, it is caused when the dermis is damaged and normal skin regeneration cannot be achieved. At this time, if the functional food composition according to the present invention is used to actively help bodily fluid circulation, it prevents inflammation and improves pain by rapidly expelling inflammatory substances or toxic substances from damaged skin, and helps the movement of immune cells to help the affected area's immune function. It strengthens and improves inflammation caused by microbial infection, and helps skin regeneration by smoothly supplying nutrients, oxygen, and moisture necessary for skin regeneration. Patches and pills using the functional food composition in the present invention help to restore the original state of the damaged skin by the above functions so as not to provide a fundamental cause for producing keloids.

<Clinical Example 12> Promoting skin cell regeneration (35 years old, male)

Photo a) After one-time management Photo b) After 4 months of patch replacement twice a week

The above person was a patient who was sent to 00 Surgery, a burn specialist in Incheon, to check if the scar caused by burns could be removed by the patch of Example 1. A patient who had been damaged by a burn for more than one year had already completed the formation of keloids, so it was impossible to reduce the scar, and the skin layer on the scar area was thickened by a skin transplant.

To this patient, a patch using the functional food composition of Example 1 was attached, and the pill was taken at a time t.i.d by 2 packets at a time. As can be seen from the photo a) of Table 9, it was observed that after 4 months (about 30 times of patch replacement), the keloid disappeared more than 95% in volume.

The principle of removing scars is not to remove keloids directly, but to promote lymphatic circulation and improve the environment of damaged dermal tissues to help normal skin cells grow well. Then, keloids cannot be formed anymore, and keloids that have already formed are peeled off over time and gradually disappear. Scar management that has already occurred requires a lot of time to be recovered from the dermal layer. In particular, burn scar management is a difficult field due to the large number of keloid formations. This improvement of burn scar gives new hope not only for burn scar but also for general scar improvement. In addition, in the case of the above person, because of nerve damage during skin transplantation, he had to wear compression socks all the time because he had to wear compression socks at all times. This is thought to be the case where not only skin cells are recovered, but also nerve cells are damaged, and it is the result of knowing how much the cell regeneration effect is when the cellular environment is normalized by promoting lymph circulation.

<Clinical Example 13> Promoting skin cell regeneration (48 years old, female)

Photo a) Before treatment Picture b) After 14 days of patch replacement once a day

Photo c) After 28 days of treatment with patch replacement once a day Photo d) After daily patch replacement 40 days after treatment

As shown in Table 10, a female patient who suffered an ankle burn in hot water was allowed to take the pills of Example 1 at a time t.i.d 3 packets at a time.

After 14 days of treatment, it was observed that not only the dermal cells but also the epidermal cells were partially regenerated in the entire area of the image, as can be seen in the photo b) of Table 3.

After 28 days of treatment, it was observed that the epidermal cells were regenerated to a considerable extent in the entire area of the burned area, as can be seen in the photo c) of Table 3.

After 40 days of treatment, it was observed that skin cells in the entire area of the burn area were regenerated, as shown in photo d) of Table 3. In addition, almost no burn scar (keloid) was observed even in severe burns.

As described above, the composition according to the present invention basically improves the environment around skin cells by smoothing lymph circulation in the skin, helps to restore the metabolism of skin cells normally, and allows the skin cells to function normally. To make damaged skin cells regenerate.

14) Clinical trial 14-Bruise removal (48 years old, female)

This patient had a dark red color due to bruising due to the rupture of blood vessels under the eyes during the procedure. In the case of this patient, the bruise was severe in the hospital, and it was diagnosed that it would take about 3-6 months to remove the bruise. After taking the functional food composition according to the present invention in t.i.d for 7 days at a time of 2 packets, the bruise was removed so that it was hardly observed with the naked eye. According to the principle of lymph circulation, bruises easily disappear when lymph circulation is promoted.

15) Clinical trial 15-Bruise removal (32 years old, female)

It was a female patient who was bruised all over her eyes with a bruise. The blood bruise-removing functional food composition pill according to the present invention was taken for 5 days in t.i.d by 2 packets at a time. It was observed with the naked eye that all other bruises disappeared, leaving only traces of small spots that could not be seen unless you look closely. Since bruises caused by bruises disappear by circulation of body fluids (lymph fluid), rapid removal of bruises confirms the effect of promoting lymphatic circulation.

16) Clinical trial 16-Body temperature control (50 years old, female)

She is a middle-aged woman with menopausal symptoms who feels very tired for no reason, her buttocks and stomach are cold, and her back is hot, and when she wakes up in the morning she feels cold all over her body. The functional food composition according to the present invention was taken for 1 month in b.i.d. After January, he stated that his buttocks and stomach were less cold, his back disappeared, and he no longer felt cold when he woke up in the morning. Above all, he stated that he no longer felt fatigue and found vitality in his body. When lymphatic circulation is promoted anatomically, it can be explained that local cold air disappears, and the lymphatic circulation promoting effect of the present invention can be confirmed through such clinical examples.

17) Clinical trial 17-Facilitating drug delivery (45, female)

This patient was a chronic cystitis patient. Septrin Tab (antibiotics for the treatment of acute chronic cystitis, pyelonephritis, urethritis, and prostatitis) was taken when cystitis was severe. However, there were frequent cases of not showing any effect even after taking Septrin Tab. This patient was allowed to take the functional food composition according to the present invention together with Septrin Tab. Subsequently, this patient stated that the effect of taking Septrin Tab appeared quickly, and that symptom improvement was clearly and clearly seen compared to before. According to the present invention, lymph circulation was promoted, and the active ingredient of Septrin Tab moved quickly and reliably to the bladder.

18) Clinical trial 18-Drug delivery promotion (68 years old, male)

It was a patient with a relatively wide area of shingles around the mouth. Aciclovir ointment was prescribed at the hospital and applied for 1 week, but there was no improvement at all. Again, I was prescribed Famclean Tab, an intravenous drug at the hospital, and took one tablet every day for one week, but the symptoms did not improve. The functional food composition according to the present invention was taken with 2 packets each time Famclean Tab was taken. After 2 days, it was observed with the naked eye that the shingles disappeared cleanly, leaving only traces. These results can be predicted through the lymphatic circulation promoting effect of the present invention.

As described above, these various effects can only be explained by the lymphatic circulation promoting effect both anatomically and pathologically. Therefore, it is possible to confirm the lymphatic circulation promoting effect of the present invention by confirming these individual effects.

<Manufacturing and marketing>

The functional food composition according to the present invention was prepared in the following composition ratios and packaging units.

A mixed powder of 27% by weight of Chungoong powder, 14% by weight of crust powder, 11% by weight of defrosted skin powder, and 8% by weight of Uuiin powder was made, and 26% by weight of maple syrup and 14% by weight of tabasheer were further mixed to make a small 3.8mm in diameter. After making a lyophilized ring, it was packaged with a stick of 4.8 g.

1 is a photograph of product contents of a functional food composition for promoting lymphatic circulation according to the present invention, and FIG. 2 is a photograph of a product packaging of a functional food composition for promoting lymphatic circulation according to the present invention.

From May 1, 2017 to March 31, 2018, more than 6,000,000 stick packages (4.8g per stick package) were sold through 2,000 pharmacies in Korea. As a result of tracking the results of administration to 2,000 edema patients through pharmacists participating in the sales, 1,960 (about 98%) patients had a weight loss effect of 1.0% to 5.0% along with edema relief.

As described above, the effect of improving edema is directly related to the effect of promoting lymphatic circulation both anatomically and pathologically. In addition, since edema is caused by stagnation of body fluids, and stagnation of body fluids is caused by lymphatic circulation disorders, the improvement in edema means that lymphatic circulation is improved. Therefore, it is possible to confirm the lymphatic circulation promoting effect of the present invention by confirming the effect of improving such edema.

Claims (3)

A functional food composition for promoting lymphatic circulation, comprising maple syrup, tabasheer, haedongpi, cinnamon, crust and uiyiin in the form of powder, liquid or extract. delete delete

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