KR102229831B1 - A Composition for extracting effective ingredients from plant comprising deep eutectic solvent and a method for extracting effective ingredients from plant using the same - Google Patents

Abstract

It relates to an extraction composition for extracting an active ingredient from a plant containing a eutectic solvent, and a method of extracting the active ingredient from a plant using the extraction composition, wherein the composition can be easily prepared by a simple manufacturing process, and the reaction There is no need to use an additional organic solvent in the process, and as no organic solvent is used, there is no emission of toxic substances and no by-products, so it is expected that there will be less emission of environmental pollutants. In addition, active ingredients can be extracted from various plants with excellent purity and yield. Accordingly, the extraction composition for extracting an active ingredient from a plant containing the eutectic solvent according to the present invention can be usefully used as an eco-friendly and economical extraction solvent replacing the extraction solvent of the conventional organic solvent.

Description

A composition for extracting effective ingredients from plant comprising deep eutectic solvent and a method for extracting effective ingredients from plant using the same}

It relates to an extraction composition for extracting an active ingredient from a plant containing a eutectic solvent, and a method of extracting the active ingredient from a plant using the extraction composition.

Polyphenols are natural antioxidants of plants, especially natural plants, and play an important role in human health because of their antioxidant enzyme activity and antioxidant enzyme activator, as well as the prooxidation metal chelation of free radical metal ions in the body. For the above reasons, many studies have been conducted on the use of waste products and by-products with polyphenols. Research has largely focused on the development of an effective and cost-effective downstream process, which aims to produce products on the basis of extracts or purification of extracts from the point of view of being used in the manufacture of foods, food supplements, cosmetics and pharmaceuticals. Most of them do.

Specifically, the existing extraction techniques for recovering polyphenol compounds from natural plants are usually solid-liquid leaching using volatile organic compounds (VOCs) such as ethanol, methanol or acetone solutions as extraction solvents. . However, VOC solvents are not beneficial to consumers or the environment due to their toxicity, high variability and non-renewable properties, and international law, including REACH, strictly regulates their use.

Thus, there is a growing need to improve the efficiency of downstream processes to upgrade food waste materials to valuable products, including the production of natural polyphenol antioxidants of commercial value, while looking for alternative green solvents to reduce environmental impact. I'm doing it.

On the other hand, as a new type of solvent formed by mixing with a solid material with a high melting point, a new solvent system called "Deep Eutectic Solvents" composed of natural materials and renewable starting materials has been developed (Chem. Commun., 2003). ,0, 70-71), the eutectic solvent is generally composed of a mixture of a hydrogen bond donor (HBD) and a hydrogen bond acceptor (HBA), and the eutectic solvent according to the combination of HBD and HBA The chemical and physical properties of are determined.

Korean Patent Publication 1020150070610

One object of the present invention is to provide an extraction composition for extracting an active ingredient from a plant.

Another object of the present invention is to provide a method for extracting an active ingredient from a plant.

To achieve the above object,

According to one aspect of the present invention, water, ethylene glycol (EG), urea (Urea), lactic acid (LA), glycerol (GLycerol, GL), oxalic acid (OA), and at least one hydrogen bond donor (HBD) selected from the group consisting of n-butyl alcohol (BA); And

At least one hydrogen bond receptor selected from the group consisting of choline chloride (ChCl), betaine (BT), tetrabutylammonium bromide (TBAB), and tetramethylammonium chloride (TMAC) ( An extraction composition for extracting an active ingredient from a plant comprising a eutectic solvent composed of a mixture of hydrogen bond acceptor, HBA) is provided.

According to another aspect of the invention,

Preparing a plant (Step 1); And

An extraction composition for extracting an active ingredient from a plant containing the eutectic solvent, and a method of extracting an active ingredient from a plant comprising the step of extracting the active ingredient by mixing the plant prepared in step 1 is provided.

The extraction composition for extracting the active ingredient from the plant containing the eutectic solvent according to the present invention can be easily prepared by a simple manufacturing process, and there is no need to use an additional organic solvent in the reaction process, and the organic solvent is not used. Accordingly, it is expected that there will be little emission of environmental pollutants because there is no emission of toxic substances or by-products. In addition, active ingredients can be extracted from various plants with excellent purity and yield. Accordingly, the extraction composition for extracting an active ingredient from a plant containing the eutectic solvent according to the present invention can be usefully used as an eco-friendly and economical extraction solvent replacing the extraction solvent of the conventional organic solvent.

FIG. 1 shows the results of extracting caffeic acid, ferulic acid, and rutin from orange peel using an extraction composition for extracting an active ingredient from a plant containing the eutectic solvent of Example 1-13 of the present invention. . (DES-1: Example 1, DES-2: Example 2, DES-3: Example 3, DES-4: Example 4, DES-5: Example 5, DES-6: Example 6, DES -7: Example 7, DES-8: Example 8, DES-9: Example 9, DES-10: Example 10, DES-11: Example 11, DES-12: Example 12, DES-13 : Example 13, MeOH: Comparative Example 1)

Hereinafter, the present invention will be described in detail.

One aspect of the present invention,

Water, ethylene glycol (EG), urea, lactic acid (LA), glycerol (GL), oxalic acid (OA), and n-butyl alcohol alcohol, BA) at least one hydrogen bond donor selected from the group consisting of (Hydrogen bond donor, HBD); And

At least one hydrogen bond receptor selected from the group consisting of choline chloride (ChCl), betaine (BT), tetrabutylammonium bromide (TBAB), and tetramethylammonium chloride (TMAC) ( It provides an extraction composition for extracting an active ingredient from a plant comprising a eutectic solvent consisting of a mixture of hydrogen bond acceptor, HBA).

The plant is not particularly limited, but the plant is orange peel, green tea, Injinho (Herba Artemisiae Scopariae), Dansam (Salvia miltiorrhiza Bge), Ginkgo biloba L., and honeysuckle vine. It may be any one selected from the group consisting of (Lonicera japonica), fennel seed, seaweed, and liquorice.

The active ingredient is contained in the plant and refers to an ingredient that exhibits specific effects, such as an antioxidant effect, a whitening effect, and an inflammation treatment effect, and the active ingredient is caffeic acid, ferulic acid ( Ferulic acid), rutin, chlorogenic acid, and glabridin may be at least one selected from polyphenol compounds consisting of, but is not limited thereto.

The hydrogen bond donor (HBD) may be ethylene glycol (EG).

The hydrogen bond acceptor (HBA) may be tetramethylammonium chloride (TMAC).

In the composition, a hydrogen bond donor (HBD) may be included in a concentration of 1 to 4 molar to 1 molar concentration of a hydrogen bond acceptor (HBA).

If a hydrogen bond donor (HBD) is contained in less than 1 molar concentration per 1 molar concentration of a hydrogen bond acceptor (HBA), the melting point is higher than room temperature and the viscosity increases. And, when it is included in an amount exceeding 4 molar concentration, there may be a problem in that the extraction efficiency decreases when used as an extraction solvent.

The extraction composition for extracting the active ingredient from the plant containing the eutectic solvent according to the present invention can be easily prepared by a simple manufacturing process, and there is no need to use an additional organic solvent in the reaction process, and the organic solvent is not used. Accordingly, it is expected that there will be little emission of environmental pollutants because there is no emission of toxic substances or by-products. In addition, it is possible to extract the active ingredient from various plants with excellent purity and yield (see Experimental Examples 1 and 2).

Accordingly, the extraction composition for extracting an active ingredient from a plant containing the eutectic solvent according to the present invention can be usefully used as an eco-friendly and economical extraction solvent replacing the extraction solvent of the conventional organic solvent.

An extraction composition for extracting an active ingredient from a plant containing the eutectic solvent,

Water, ethylene glycol (EG), urea, lactic acid (LA), glycerol (GL), oxalic acid (OA), and n-butyl alcohol alcohol, BA) at least one hydrogen bond donor selected from the group consisting of (Hydrogen bond donor, HBD); And at least one hydrogen bond receptor selected from the group consisting of choline chloride (ChCl), betaine (BT), tetrabutylammonium bromide (TBAB), and tetramethylammonium chloride (TMAC). Mixing (Hydrogen bond acceptor, HBA) (Step 1);

Heating the mixture of step 1 (step 2); And

Drying the heated mixture of step 2 (step 3); It can be prepared through a method for producing an extraction composition for extracting the active ingredient from the plant containing the eutectic solvent.

Hereinafter, the manufacturing method will be described in detail.

In the method for preparing an extraction composition for extracting an active ingredient from a plant of the present invention, step 1 is a step of mixing a hydrogen bond donor and a hydrogen bond acceptor. In this case, a hydrogen bond donor (HBD) may be used in a concentration of 1 to 4 molar to 1 molar concentration of the hydrogen bond acceptor (HBA).

In the method for preparing an extraction composition for extracting an active ingredient from a plant of the present invention, step 2 is a step of heating the mixture of step 1.

The heating of step 2 may be performed at 60 °C to 120 °C, and may be performed at 80 °C.

In addition, the heating in step 2 may be performed for 0.5 to 2 hours.

In the method for preparing an extraction composition for extracting an active ingredient from a plant of the present invention, step 3 is a step of drying the heated mixture of step 2. The drying may be performed using an oven, may be performed at 80 °C to 120 °C, and may be performed at 80 °C.

In addition, drying in step 3 may be performed for 12 hours.

A specific method of manufacturing an extraction composition for extracting an active ingredient from the plant may be carried out by performing a procedure similar to Examples 1-14 below, but this is only an example and is not limited thereto.

Another aspect of the present invention,

Preparing a plant (Step 1); And

It provides a method for extracting an active ingredient from a plant comprising the step of extracting the active ingredient by mixing the composition for extracting the active ingredient from the plant containing the eutectic solvent, and the plant prepared in step 1 above.

At this time, the extraction in step 2 may be used without limitation as long as it is a method that can be extracted using a solution, but it can be extracted using methods such as ultrasonic treatment (Ultra sonic), dipping, and reflux. have.

Hereinafter, the present invention will be described in detail by examples and experimental examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following Examples and Experimental Examples.

<Example 1> Preparation of eutectic solvent (DES-1)

Mix 12.4 g (0.2 mol) of ethylene glycol (EG) and 1.8 g (0.1 mol) of water with 14.00 g (0.1 mol) of choline chloride (ChCl) and heat to 80 °C to completely If held for 1 hour until dissolved, a colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-1 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 2> Preparation of eutectic solvent (DES-2)

Mix 18 g (0.2 mol) of lactic acid (LA) and 1.8 g (0.1 mol) of water with 14.00 g (0.1 mol) of choline chloride (ChCl) and heat to 80 °C to completely dissolve the material. If it is kept for 1 hour until it becomes clear, a colorless and transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-2 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 3> Preparation of eutectic solvent (DES-3)

Mix ethylene glycol (EG) 24.8 g (0.4 mol) and choline chloride (ChCl) 14.00 g (0.1 mol) together and heat to 80 °C for 1 hour until the material is completely dissolved When done, a colorless and transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-3 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 4> Preparation of eutectic solvent (DES-4)

Ethylene glycol (EG) 18.6 g (0.3 mol) and choline chloride (ChCl) 14.00 g (0.1 mol) are mixed together and heated to 80 °C for 1 hour until the material is completely dissolved. When done, a colorless and transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-4 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 5> Preparation of eutectic solvent (DES-5)

12.4 g (0.2 mol) of ethylene glycol (EG) and 14.00 g (0.1 mol) of choline chloride (ChCl) are mixed together and heated to 80 °C for 1 hour until the material is completely dissolved. When done, a colorless and transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-5 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 6> Preparation of eutectic solvent (DES-6)

When lactic acid (LA) 18 g (0.2 mol) and choline chloride (ChCl) 14.00 g (0.1 mol) are mixed together and heated to 80 °C, the material is kept for 1 hour until completely dissolved. A colorless transparent liquid is produced. After the material was completely finished, the eutectic solvent DES-6 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 7> Preparation of eutectic solvent (DES-7)

When 18 g (0.2 mol) of oxalic acid (OA) and 14.00 g (0.1 mol) of choline chloride (ChCl) are mixed together, heated to 80 °C, and maintained for 1 hour until the material is completely dissolved. A colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-7 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 8> Preparation of eutectic solvent (DES-8)

When 18.4 g (0.2 mol) of lactic acid (LA) and 14.00 g (0.1 mol) of choline chloride (ChCl) are mixed together, heated to 80 °C and held for 1 hour until the material is completely dissolved. A colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-8 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 9> Preparation of eutectic solvent (DES-9)

14.8 g (0.2 mol) of n-butyl alcohol (BA) and 14.00 g (0.1 mol) of choline chloride (ChCl) are mixed together and heated to 80 °C until the material is completely dissolved. If held for 1 hour, a colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-9 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 10> Preparation of eutectic solvent (DES-10)

It is colorless when mixed with lactic acid (LA) 18.4 g (0.2 mol) and betaine (BT) 11.7 g (0.1 mol) and heated to 80 °C for 1 hour until the material is completely dissolved. A clear liquid is produced. After the reaction was complete, the eutectic solvent DES-10 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 11> Preparation of eutectic solvent (DES-11)

Mix 18.4 g (0.2 mol) of lactic acid (LA) and 32.2 g (0.1 mol) of tetrabutylammonium bromide (TBAB) together and heat to 80 °C for 1 hour until the material is completely dissolved. If maintained, a colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-11 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 12> Preparation of eutectic solvent (DES-12)

Mix 18.4 g (0.2 mol) of lactic acid (LA) and 10.9 g (0.1 mol) of tetramethylammonium chloride (TMAC) together and heat to 80 °C for 1 hour until the material is completely dissolved. If maintained, a colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-12 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 13> Preparation of eutectic solvent (DES-13)

12.4 g (0.2 mol) of ethylene glycol (EG) and 10.9 g (0.1 mol) of tetramethylammonium chloride (TMAC) are mixed together and heated to 80 °C for 1 hour until the material is completely dissolved. If held for a while, a colorless transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-13 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

<Example 14> Preparation of eutectic solvent (DES-14)

Mix with ethylene glycol (EG) 12.4 g (0.2 mol) and urea 6 g (0.1 mol) tetramethylammonium chloride (TMAC) 10.9 g (0.1 mol) and heat to 80 °C Thus, if the material is kept for 1 hour until completely dissolved, a colorless and transparent liquid is produced. After the reaction was complete, the eutectic solvent DES-14 was prepared by drying in an oven at 80°C for 12 hours to obtain a viscous liquid.

The composition of the ternary deep eutectic solvent prepared in Examples 1, 2 and 14 is shown in Table 1 below.

Example HBA HBD 1 HBD 2 Molar/ratio
(HBA/HBD1/HBD2) One ChCl EG Water 1/2/1 2 ChCl EG LA 1/2/1 14 TMAC EG Urea 1/2/1

In the above table, HBA is a hydrogen bond acceptor, and HBD is a hydrogen bond donor.

The composition of the binary deep eutectic solvent prepared in Example 3-13 is shown in Table 2 below.

Example HBA HBD Molar/ratio
(HBA/HBD) 3 ChCl EG 1/4 4 ChCl EG 1/3 5 ChCl EG 1/2 6 ChCl LA 1/2 7 ChCl OA 1/2 8 ChCl GL 1/2 9 ChCl BA 1/2 10 BT LA 1/2 11 TBAB LA 1/2 12 TMAC LA 1/1 13 TMAC EG 1/2

In the above table, HBA is a hydrogen bond acceptor, and HBD is a hydrogen bond donor.

Table 3 below summarizes the plants used in the following experimental examples and the active ingredients extracted from each plant.

Plant Extracted active ingredient Orange peel
Green tea leaves
Injinho (Herba Artemisiae Scopariae) leaves
Salvia miltiorrhiza Bge root
Ginkgo biloba L. leaves
Honeysuckle (Lonicera japonica) stem
Fennel seed
Seaweed leaves <Antioxidant quality>
Caffeic acid
Ferulic acid
Rutin
Chlorogenic acid Liquorice root <Whitening substance>
Glabridin

<Experimental Example 1> Extraction of active ingredients from orange peel using an extraction composition for extracting active ingredients from plants containing eutectic solvent

In order to examine the extraction efficiency of the extraction composition for extracting the active ingredient from the plant containing the eutectic solvent according to the present invention, the active ingredient was extracted from various plants using the composition of Examples 1-14. As a comparative example, the same experiment was performed using methanol as an organic solvent.

The specific extraction method is as follows.

0.1 g of plants (green tea leaves, injinho leaves, sweet ginseng roots, ginkgo leaves, honeysuckle stems, fennel seeds, seaweed leaves, sensitive grass roots) and 1 ml of the eutectic solvent of Example 1-13 at 40 °C and 20 rpm for 30 minutes After mixing, put the prepared mixture into a syringe attached with a filter 0.45 (μm) and extract the extract.

Using the composition of Example 1-13 as an extraction solvent, caffeic acid, ferulic acid, and rutin were extracted from orange peel, and the extraction yield was summarized and shown in FIG. 1.

As shown in Figure 1,

It can be seen that caffeic acid, ferulic acid, and rutin can be extracted from orange peel by using the composition for extraction for extracting the active ingredient from the plant containing the eutectic solvent of the present invention as an extraction solvent. In particular, it can be seen that the composition of Example 13 exhibits the best extraction efficiency among the examples, and significantly superior extracting amount of active ingredients than methanol, an organic solvent widely used as an extraction solvent.

In terms of extracting active ingredients,

Ferulic acid, when using the compositions of Examples 3, 4, 7, 9, and 13 as an extraction solvent, it can be seen that 10 mg / g or more exhibits an extraction amount similar to methanol, an organic solvent widely used as an extraction solvent, In particular, in the case of using Example 13, the extraction amount was higher than that of methanol, and it was found that the extraction efficiency of ferulic acid was more excellent.

Caffeic acid, when using the composition of Examples 2, 3, 4, 8, and 13 as an extraction solvent, exhibits a higher extraction amount than methanol, and in particular, when using Example 13, the extraction amount is about 1.5 times higher than that of methanol. By showing, it can be seen that the extraction efficiency of caffeic acid is remarkably excellent.

Rutin, when using Example 13, showed an extraction amount that is about 1.5 times higher than that of methanol, and it can be seen that the extraction efficiency of rutin was remarkably excellent.

<Experimental Example 2> Extraction of active ingredients from various plants using an extraction composition for extracting active ingredients from plants containing eutectic solvent

In the results of FIG. 1, using the composition of Example 13, which is an extraction solvent showing the highest extraction effect with respect to caffeic acid, ferulic acid, and rutin, orange peel, green tea, injinho, dansam, ginkgo, honeysuckle and fennel Caffeic acid, ferulic acid, and rutin were extracted from, and the extraction yield using Example 13 and the extraction yield extracted using methanol were compared with specific values, and are shown in Table 4 below.

In addition, chlorogenic acid was extracted from various plants using Example 13 as an extraction solvent, and the extraction yield using Example 13 and the extraction yield extracted using ethanol were compared with specific values, and are shown in Table 5 below.

In addition, examples 13 and 14 were used as extraction solvents to extract glabridin from the sensitive weeds, and the extraction yields using Examples 13 and 14 and the extraction yields extracted using ethanol were compared with specific values in Table 6 below. Indicated.

The specific experimental method of each experiment was performed in the same manner as in Experimental Example 1.

Extraction yield (mg/g) Caffeic acid Ferulic acid Routine Plant MeOH Example 13 MeOH Example 13 MeOH Example 13 orange peel 1.77 3.18 11.8 12.24 0.75 1.29 green tea 0.26 0.48 0.24 0.59 0.63 1.50 Jinho In 0.25 0.46 0.39 0.47 0.25 0.48 Dansam 0.17 0.28 0.41 0.84 0.56 0.62 Ginkgo 1.15 1.89 0.75 1.08 1.12 1.53 Seahorse 0 0 0.55 0.86 0 0 Honeysuckle 0.22 0.41 0.26 0.61 0.47 0.69 fennel 0.12 0.15 0 0 0.13 0.18

As shown in Table 4 above,

It can be seen that the extraction composition for extracting the active ingredient from the plant containing the eutectic solvent of the present invention can extract the active ingredient regardless of the type of the plant. In addition, it can be seen that the extraction amount of the active ingredient is significantly better than methanol, which is an organic solvent widely used as an extraction solvent.

Chlorogenic acid extraction yield (mg/g) Plant EtOH Example 13 green tea 0.092 0.17 Jinho In 0.49 2.20 Ginkgo 0.13 0.15 Honeysuckle 0.085 0.099 Sensitive herb 0.88 4.3 fennel 0.11 0.23 orange peel 0.13 0.49

As shown in Table 5 above,

It can be seen that the extraction composition for extracting the active ingredient from the plant containing the eutectic solvent of the present invention can extract the active ingredient regardless of the type of the plant. In addition, it can be seen that the extraction amount of the active ingredient is significantly better than ethanol, which is an organic solvent widely used as an extraction solvent.

Extraction yield Glabridin (mg/g) Plant EtOH Example 13 Example 14 Sensitive herb 0.10 0.063 0.10

As shown in Table 6 above,

It can be seen that the extraction composition for extracting the active ingredient from the plant containing the eutectic solvent of the present invention can extract the active ingredient glabridin, which is well known as a whitening substance, from the sensitive herb. Particularly, in the case of Example 13, it can be seen that the extraction amount of the active ingredient is significantly better than ethanol, which is an organic solvent widely used as an extraction solvent.

As mentioned above,

The extraction composition for extracting the active ingredient from the plant containing the eutectic solvent according to the present invention can be easily prepared by a simple manufacturing process, and there is no need to use an additional organic solvent in the reaction process, and the organic solvent is not used. Accordingly, it is expected that there will be little emission of environmental pollutants because there is no emission of toxic substances or by-products. In addition, active ingredients can be extracted from various plants with excellent purity and yield.

Accordingly, the extraction composition for extracting an active ingredient from a plant containing the eutectic solvent according to the present invention can be usefully used as an eco-friendly and economical extraction solvent replacing the extraction solvent of the conventional organic solvent.

Claims (10)

Orange peel, Green tea, Herba Artemisiae Scopariae, Salvia miltiorrhiza Bge, Ginkgo biloba L., Lonicera japonica, Fennel seed and sea horse (seaweed) preparing any one plant selected from the group consisting of; And
Hydrogen bond donor (HBD), which is ethylene glycol (EG); And extracting an active ingredient by mixing an extraction solvent consisting of a eutectic solvent composed of a mixture of a hydrogen bond acceptor (HBA), which is tetramethylammonium chloride (TMAC), and the plant,
The active ingredient is at least one selected from polyphenol compounds consisting of caffeic acid, ferulic acid, rutin, and chlorogenic acid. .
delete delete delete delete The method of claim 1,
A method for extracting an active ingredient from a plant comprising 1 to 4 molar concentrations of a hydrogen bond donor (HBD) to 1 molar concentration of the hydrogen bond acceptor (HBA) .
delete delete delete The method of claim 1,
The extraction method for extracting an active ingredient from a plant, characterized in that it is carried out through one type of method selected from the group consisting of ultrasonic treatment (Ultra sonic), dipping (Dipping) and reflux (Reflux).

Images