CN106577829A - Plant composition for black spot disease of Stevia rebaudiana and preparation method thereof - Google Patents

Plant composition for black spot disease of Stevia rebaudiana and preparation method thereof Download PDF

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Publication number
CN106577829A
CN106577829A CN201610949877.4A CN201610949877A CN106577829A CN 106577829 A CN106577829 A CN 106577829A CN 201610949877 A CN201610949877 A CN 201610949877A CN 106577829 A CN106577829 A CN 106577829A
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Prior art keywords
parts
melasma
radix
stevlae rebaudianae
folium stevlae
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CN201610949877.4A
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Chinese (zh)
Inventor
贾德军
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Mingguang City A Total Of Stevia Professional Cooperatives
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Mingguang City A Total Of Stevia Professional Cooperatives
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Priority to CN201610949877.4A priority Critical patent/CN106577829A/en
Publication of CN106577829A publication Critical patent/CN106577829A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/36Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention provides a plant composition for black spot disease of Stevia rebaudiana and a preparation method thereof. The plant composition comprises the following components in parts by weight: 18-25 parts of toosendan fruits, 12-18 parts of giant knotweed rhizomes, 15-20 parts of bupleurum roots, 17-23 parts of thunder god vine roots, 17-25 parts of Aconitum coreanum, 18-26 parts of dichroa leaves, 18-27 parts of cornus fruits, 15-22 parts of soapberries, 15-20 parts of garden burnet roots, 18-26 parts of nux vomica, 12-18 parts of ginkgo testa, and 15-20 parts of phellodendron barks; and each component is crushed and extracted in order to obtain the composition. The plant composition for black spot disease of Stevia rebaudiana employs pure natural plants as raw materials, the composition is green and environmentally protected without pollution, the preparation method is simple and controllable, the composition can be effectively used for controlling black spot disease of Stevia rebaudiana, and the composition can be widely applied to control of black spot disease of Stevia rebaudiana.

Description

A kind of vegetable composition for Folium Stevlae Rebaudianae melasma and preparation method thereof
Technical field
The present invention relates to Folium Stevlae Rebaudianae planting technology field, in particular it relates to a kind of plant group for Folium Stevlae Rebaudianae melasma Compound and preparation method thereof.
Background technology
Folium Stevlae Rebaudianae is Compositae Stevia herbaceos perennial.The whole strain of Folium Stevlae Rebaudianae all contains sugar, with the sugariness of blade most Height, containing glucosides about 14%, the glucosides of branch stalk can work as sugar material after the solarization dry grinding of Folium Stevlae Rebaudianae vanes equivalent to the half of blade, or Jing ethanol precipitatings obtain stevioside, 250-300 times equivalent to white sugar of sugariness, and heat is only the 1/300 of white sugar, Moreover, stevioside has no toxic side effect, and without carcinogen, edible safety, Jing often to eat is used can prophylaxis of hypertension, diabetes, obesity The diseases such as disease, heart disease, dental caries, are a kind of ideal sweeting agents of replacement sucrose.At present, food, drink are widely used to The industry such as material, medicine, daily-use chemical industry, wine brewing, cosmetics, and relatively apply sucrose escapable cost 60%.
Folium Stevlae Rebaudianae melasma is one of Major Diseases in Folium Stevlae Rebaudianae planting process, is typically fallen ill in the height of peak of growing season Temperature, high humidity season, the angle speckle of the pitchy of middle and lower part blade presentation before this, and it is rapid to full leaf extension, morbidity later stage fallen leaves, yield Loss causes serious economic loss to Planting household up to more than 50%.At present, entered using chemical pesticide more than Folium Stevlae Rebaudianae melasma Row preventing and treating, but life-time service, pathogen can produce certain Drug resistance, and this can cause chemical pesticide usage amount to increase so that agriculture Medicine residual is serious, also endangers health of people while pollution environment.As people are got over to the demand of pollution-free food, pollution-free food Come higher, with low toxicity, nontoxic, degradable, to the little medicine based on herbs of impact of ecological environment by before with good exploitation Scape and using value.
The content of the invention
For the defect that prior art is present, it is an object of the invention to provide a kind of environmental protection, it is eco-friendly for Vegetable composition of Folium Stevlae Rebaudianae melasma and preparation method thereof.
To reach above-mentioned purpose, the present invention is adopted the following technical scheme that:A kind of plant societys for Folium Stevlae Rebaudianae melasma Thing, including the component of following weight portion:18~25 parts of Fructus Toosendan, 12~18 parts of Rhizoma Polygoni Cuspidati, 15~20 parts of Radix Bupleuri, Radix Tripterygii Wilfordii 17~23 Part, 17~25 parts of Radix Aconiti Coreani, 18~26 parts of Ramulus et Folium Dichroae febrifugae, 18~27 parts of Fructus Corni, 15~22 parts of Fructus Sapindi Mukouossi, 15~20 parts of Radix Sanguisorbae, horse 18~26 parts of Semen Strychni, 12~18 parts of gingko episperm, 15~20 parts of Cortex Phellodendri.
Preferably, the vegetable composition for Folium Stevlae Rebaudianae melasma, including the component of following weight portion:Fructus Toosendan 22 Part, 16 parts of Rhizoma Polygoni Cuspidati, 17 parts of Radix Bupleuri, 20 parts of Radix Tripterygii Wilfordii, 22 parts of Radix Aconiti Coreani, 23 parts of Ramulus et Folium Dichroae febrifugae, 24 parts of Fructus Corni, 20 parts of Fructus Sapindi Mukouossi, ground 18 parts of elm, 23 parts of Semen Strychni, 15 parts of gingko episperm, 16 parts of Cortex Phellodendri.
A kind of preparation method of the vegetable composition as described for Folium Stevlae Rebaudianae melasma, comprises the steps:
(1)To plant outside Fructus Toosendan, Rhizoma Polygoni Cuspidati, Radix Bupleuri, Radix Tripterygii Wilfordii, Radix Aconiti Coreani, Ramulus et Folium Dichroae febrifugae, Fructus Corni, Fructus Sapindi Mukouossi, Radix Sanguisorbae, Semen Strychni, Semen Ginkgo Skin, Cortex Phellodendri are ground into 40~50 mesh;
(2)Taking the component after the above-mentioned crushing of weight proportion part adds the ethanol of its weight 8-10 times to extract, and filters, takes filtrate, Extract three times, merging filtrate obtains filtrate;
(3)By the step(2)In filtrate be concentrated into the 20%~25% of stock solution, add concentration after 10~15 times of stoste volume Water be diluted, after mix homogeneously, be heated to without alcohol taste, stand 10-12h, filter, obtain final product described for Folium Stevlae Rebaudianae melasma Vegetable composition.
Preferably, the step(2)In Extracting temperature be 70~80 DEG C.
Preferably, the step(2)In ethanol used be 80~95% ethanol.
Preferably, the step(2)In ethanol used be 90% ethanol.
It is, with Folium Stevlae Rebaudianae is sprayed on after 300-500 times of water dilution, to spray which per 7 days for preventing and treating Folium Stevlae Rebaudianae melasma Spill once, continuous 3 times.
Compared with prior art, the present invention has following beneficial effect:It is of the present invention for Folium Stevlae Rebaudianae melasma Vegetable composition using pure natural plant as raw material, green pollution-free, its preparation method are simply controllable, and can be effective Preventing and treating Folium Stevlae Rebaudianae melasma, can be widely applied to the preventing and treating of Folium Stevlae Rebaudianae melasma.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention Rather than limit the scope of the present invention.
Embodiment 1
It is used for the vegetable composition of Folium Stevlae Rebaudianae melasma, including the component of following weight portion described in the present embodiment:22 parts of Fructus Toosendan, 16 parts of Rhizoma Polygoni Cuspidati, 17 parts of Radix Bupleuri, 20 parts of Radix Tripterygii Wilfordii, 22 parts of Radix Aconiti Coreani, 23 parts of Ramulus et Folium Dichroae febrifugae, 24 parts of Fructus Corni, 20 parts of Fructus Sapindi Mukouossi, Radix Sanguisorbae 18 Part, 23 parts of Semen Strychni, 15 parts of gingko episperm, 16 parts of Cortex Phellodendri.
As described for the preparation method of the vegetable composition of Folium Stevlae Rebaudianae melasma, comprise the steps:
(1)To plant outside Fructus Toosendan, Rhizoma Polygoni Cuspidati, Radix Bupleuri, Radix Tripterygii Wilfordii, Radix Aconiti Coreani, Ramulus et Folium Dichroae febrifugae, Fructus Corni, Fructus Sapindi Mukouossi, Radix Sanguisorbae, Semen Strychni, Semen Ginkgo Skin, Cortex Phellodendri are ground into 40~50 mesh;
(2)Take the component after the above-mentioned crushing of weight proportion part add 9 times of its weight 90% ethanol and 70 DEG C at extract, filter, Filtrate is taken, is extracted three times, merging filtrate obtains filtrate;
(3)By the step(2)In filtrate be concentrated into the 20% of stock solution, after adding concentration, the water of 15 times of stoste volume is carried out Dilution, after mix homogeneously, is heated to, without alcohol taste, standing 12h, filters, obtain final product the plant societys for Folium Stevlae Rebaudianae melasma Thing.
It is, with Folium Stevlae Rebaudianae is sprayed on after 300 times of water dilutions, to spray which once per 7 days for preventing and treating Folium Stevlae Rebaudianae melasma, Continuous 3 times, the control rate of Folium Stevlae Rebaudianae melasma reaches 91%, and using the spray of 70% thiophanate methyl powder of chemical pesticide, 800 times of liquid The prevention effect of mist is suitable, and the vegetable composition for Folium Stevlae Rebaudianae melasma is more friendly to environment.
Embodiment 2
It is used for the vegetable composition of Folium Stevlae Rebaudianae melasma, including the component of following weight portion described in the present embodiment:25 parts of Fructus Toosendan, 12 parts of Rhizoma Polygoni Cuspidati, 15 parts of Radix Bupleuri, 17 parts of Radix Tripterygii Wilfordii, 25 parts of Radix Aconiti Coreani, 18 parts of Ramulus et Folium Dichroae febrifugae, 18 parts of Fructus Corni, 22 parts of Fructus Sapindi Mukouossi, Radix Sanguisorbae 20 Part, 18 parts of Semen Strychni, 12 parts of gingko episperm, 20 parts of Cortex Phellodendri.
As described for the preparation method of the vegetable composition of Folium Stevlae Rebaudianae melasma, comprise the steps:
(1)To plant outside Fructus Toosendan, Rhizoma Polygoni Cuspidati, Radix Bupleuri, Radix Tripterygii Wilfordii, Radix Aconiti Coreani, Ramulus et Folium Dichroae febrifugae, Fructus Corni, Fructus Sapindi Mukouossi, Radix Sanguisorbae, Semen Strychni, Semen Ginkgo Skin, Cortex Phellodendri are ground into 40~50 mesh;
(2)Take the component after the above-mentioned crushing of weight proportion part add 10 times of its weight 80% ethanol and 80 DEG C at extract, mistake Filter, takes filtrate, extracts three times, and merging filtrate obtains filtrate;
(3)By the step(2)In filtrate be concentrated into the 23% of stock solution, after adding concentration, the water of 13 times of stoste volume is carried out Dilution, after mix homogeneously, is heated to, without alcohol taste, standing 10h, filters, obtain final product the plant societys for Folium Stevlae Rebaudianae melasma Thing.
It is, with Folium Stevlae Rebaudianae is sprayed on after 400 times of water dilutions, to spray which once per 7 days for preventing and treating Folium Stevlae Rebaudianae melasma, Continuous 3 times, the control rate of Folium Stevlae Rebaudianae melasma reaches 89%, and using the spray of 70% thiophanate methyl powder of chemical pesticide, 800 times of liquid The prevention effect of mist is suitable, and the vegetable composition for Folium Stevlae Rebaudianae melasma is more friendly to environment.
Embodiment 3
It is used for the vegetable composition of Folium Stevlae Rebaudianae melasma, including the component of following weight portion described in the present embodiment:18 parts of Fructus Toosendan, 18 parts of Rhizoma Polygoni Cuspidati, 15 parts of Radix Bupleuri, 23 parts of Radix Tripterygii Wilfordii, 17 parts of Radix Aconiti Coreani, 26 parts of Ramulus et Folium Dichroae febrifugae, 27 parts of Fructus Corni, 15 parts of Fructus Sapindi Mukouossi, Radix Sanguisorbae 15 Part, 26 parts of Semen Strychni, 12 parts of gingko episperm, 15 parts of Cortex Phellodendri.
As described for the preparation method of the vegetable composition of Folium Stevlae Rebaudianae melasma, comprise the steps:
(1)To plant outside Fructus Toosendan, Rhizoma Polygoni Cuspidati, Radix Bupleuri, Radix Tripterygii Wilfordii, Radix Aconiti Coreani, Ramulus et Folium Dichroae febrifugae, Fructus Corni, Fructus Sapindi Mukouossi, Radix Sanguisorbae, Semen Strychni, Semen Ginkgo Skin, Cortex Phellodendri are ground into 40~50 mesh;
(2)Take the component after the above-mentioned crushing of weight proportion part add 8 times of its weight 95% ethanol and 75 DEG C at extract, filter, Filtrate is taken, is extracted three times, merging filtrate obtains filtrate;
(3)By the step(2)In filtrate be concentrated into the 25% of stock solution, after adding concentration, the water of 10 times of stoste volume is carried out Dilution, after mix homogeneously, is heated to, without alcohol taste, standing 11h, filters, obtain final product the plant societys for Folium Stevlae Rebaudianae melasma Thing.
It is, with Folium Stevlae Rebaudianae is sprayed on after 500 times of water dilutions, to spray which once per 7 days for preventing and treating Folium Stevlae Rebaudianae melasma, Continuous 3 times, the control rate of Folium Stevlae Rebaudianae melasma reaches 85%, and using the spray of 70% thiophanate methyl powder of chemical pesticide, 800 times of liquid The prevention effect of mist is suitable, and the vegetable composition for Folium Stevlae Rebaudianae melasma is more friendly to environment.
Above the specific embodiment of the present invention is described.It is to be appreciated that the invention is not limited in above-mentioned Particular implementation, those skilled in the art can make various modifications or modification within the scope of the claims, this not shadow Ring the flesh and blood of the present invention.

Claims (6)

1. a kind of vegetable composition for Folium Stevlae Rebaudianae melasma, it is characterised in that including the component of following weight portion:Fructus Toosendan 18 ~25 parts, 12~18 parts of Rhizoma Polygoni Cuspidati, 15~20 parts of Radix Bupleuri, 17~23 parts of Radix Tripterygii Wilfordii, 17~25 parts of Radix Aconiti Coreani, 18~26 parts of Ramulus et Folium Dichroae febrifugae, 18~27 parts of Fructus Corni, 15~22 parts of Fructus Sapindi Mukouossi, 15~20 parts of Radix Sanguisorbae, 18~26 parts of Semen Strychni, 12~18 parts of gingko episperm, 15~20 parts of Cortex Phellodendri.
2. the vegetable composition of Folium Stevlae Rebaudianae melasma is used for according to claim 1, it is characterised in that described for Folium Stevlae Rebaudianae The vegetable composition of melasma, including the component of following weight portion:22 parts of Fructus Toosendan, 16 parts of Rhizoma Polygoni Cuspidati, 17 parts of Radix Bupleuri, Radix Tripterygii Wilfordii 20 Part, 22 parts of Radix Aconiti Coreani, 23 parts of Ramulus et Folium Dichroae febrifugae, 24 parts of Fructus Corni, 20 parts of Fructus Sapindi Mukouossi, 18 parts of Radix Sanguisorbae, 23 parts of Semen Strychni, gingko episperm 15 Part, 16 parts of Cortex Phellodendri.
3. a kind of preparation method of the vegetable composition for being used for Folium Stevlae Rebaudianae melasma as described in any one of claim 1-2, which is special Levy and be, the preparation method comprises the steps:
(1)To plant outside Fructus Toosendan, Rhizoma Polygoni Cuspidati, Radix Bupleuri, Radix Tripterygii Wilfordii, Radix Aconiti Coreani, Ramulus et Folium Dichroae febrifugae, Fructus Corni, Fructus Sapindi Mukouossi, Radix Sanguisorbae, Semen Strychni, Semen Ginkgo Skin, Cortex Phellodendri are ground into 40~50 mesh;
(2)Taking the component after the above-mentioned crushing of weight proportion part adds the ethanol of its weight 8-10 times to extract, and filters, takes filtrate, Extract three times, merging filtrate obtains filtrate;
(3)By the step(2)In filtrate be concentrated into the 20%~25% of stock solution, add concentration after 10~15 times of stoste volume Water be diluted, after mix homogeneously, be heated to without alcohol taste, stand 10-12h, filter, obtain final product described for Folium Stevlae Rebaudianae melasma Vegetable composition.
4. the preparation method of the vegetable composition of Folium Stevlae Rebaudianae melasma is used for according to claim 3, it is characterised in that described Step(2)In Extracting temperature be 70~80 DEG C.
5. the preparation method of the vegetable composition of Folium Stevlae Rebaudianae melasma is used for according to claim 3, it is characterised in that described Step(2)In ethanol used be 80~95% ethanol.
6. the preparation method of the vegetable composition of Folium Stevlae Rebaudianae melasma is used for according to claim 5, it is characterised in that described Step(2)In ethanol used be 90% ethanol.
CN201610949877.4A 2016-11-03 2016-11-03 Plant composition for black spot disease of Stevia rebaudiana and preparation method thereof Withdrawn CN106577829A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942295A (en) * 2017-05-03 2017-07-14 沈荣存 A kind of preparation method for the natural bactericidal agent for preventing and treating safron black spot
CN106962419A (en) * 2017-05-03 2017-07-21 沈荣存 A kind of natural bactericidal agent for preventing and treating safron black spot and its application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1448057A (en) * 2002-04-01 2003-10-15 张水良 Dressing agent
CN104026174A (en) * 2014-06-26 2014-09-10 太仓市荣德生物技术研究所 Sterilizing agent for crops
CN104082358A (en) * 2014-06-27 2014-10-08 匡铁铃 Chinese herbal medicine pesticide
CN105815352A (en) * 2016-04-13 2016-08-03 储志强 Chinese herbal medicinal pesticide

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1448057A (en) * 2002-04-01 2003-10-15 张水良 Dressing agent
CN104026174A (en) * 2014-06-26 2014-09-10 太仓市荣德生物技术研究所 Sterilizing agent for crops
CN104082358A (en) * 2014-06-27 2014-10-08 匡铁铃 Chinese herbal medicine pesticide
CN105815352A (en) * 2016-04-13 2016-08-03 储志强 Chinese herbal medicinal pesticide

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106942295A (en) * 2017-05-03 2017-07-14 沈荣存 A kind of preparation method for the natural bactericidal agent for preventing and treating safron black spot
CN106962419A (en) * 2017-05-03 2017-07-21 沈荣存 A kind of natural bactericidal agent for preventing and treating safron black spot and its application

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