KR102220754B1 - manufacturing method of cosmetic composition including liposome powder - Google Patents

Abstract

Disclosed is a method for preparing a cosmetic composition comprising liposome powder. According to the method for manufacturing a cosmetic composition comprising liposome powder according to the present invention, it is possible to provide a more homogeneous and stable cosmetic composition by achieving change in physical properties such as particle size distribution without chemical additives, and to achieve product function improvement such as the feeling of use and adhesion of the cosmetic. can do.
In addition, it is possible to increase the absorption power of the active ingredient by changing the particle size distribution, and when preparing liposome particles by improving the dispersibility, productivity can be improved by increasing the production yield more usefully.

Description

Manufacturing method of cosmetic composition including liposome powder

The present invention relates to a method for manufacturing a cosmetic composition comprising liposome powder, and more particularly, to provide a more homogeneous and stable cosmetic composition by changing the particle size distribution in a desired direction, and to improve the feeling of use and adhesion of the cosmetic. It relates to a method for producing a cosmetic composition comprising a.

A woman's instinct to pursue beauty is not yesterday and today, but in recent years, interest in body shape management, beauty, or cosmetics to make her look more beautiful is on the rise.

And as men as well as women are interested in cosmetics for skin care, the cosmetics market is continuously expanding, and various types of cosmetics are being released on the market.

In general, cosmetics can be largely divided into basic cosmetics, makeup bases, and color cosmetics, and are manufactured as solid, liquid or gel cosmetics, and cosmetic containers storing them are also variously applied.

Recently, functional cosmetics that can perform various functions such as moisturizing, whitening, wrinkle improvement, UV protection, acne relief, atopy relief, anti-inflammatory or keratin dissolution are also widely consumed.

Meanwhile, skin cells often supply substances required by cells from the outside in order to maintain life phenomena or improve problems. Accordingly, formulation technology using a drug delivery system (DDS) is constantly being studied as a method to efficiently move an effective substance desired by cells into cells in the industrial field related to cosmetics or pharmaceuticals.

In addition, in order to apply this to cosmetics, a stabilization technology that does not deteriorate during the manufacturing and distribution process and maintains its original activity is indispensable.

Recently, a capsule formulation is mentioned as a transdermal absorption system that can be absorbed into the skin most stably while protecting the active ingredients of cosmetics.

The carriers of these encapsulations are classified into Microsphere (MIS), Microcapsules (MIC), Liposome (LIP), etc. according to their properties and origin. Among these, liposomes are effectively used to stably absorb natural physiologically active ingredients used as raw materials for functional cosmetics as carriers of various drugs into skin cells.

Specifically, liposome formulations are a concept of encapsulation that can contain useful cosmetic ingredients, and are in the spotlight as a medium that delivers natural ingredients for the efficacy of whitening anti-wrinkle anti-aging to the skin relatively stably, and is currently in the form of grafting with nano cosmetic formulations. It is also being studied continuously.

As a prior art for a cosmetic composition using such a liposome formulation, there is Korean Patent No. 10-0654102 and the like.

(Patent Document 1) KR10-0654102 B1

However, in general, when the size of the liposome is too large, the stability decreases, whereas when the size is too small, the capacity of the nutrient decreases, so it is important to be prepared to have a homogeneous distribution with an appropriate size.

In addition, liposomes have problems such as physical instability of the lipid bilayer membrane constituting the liposome, low emulsion stability, and low capture efficiency of active ingredients. In addition, there are many cases in which the effect of the active substance is limited because the skin penetration of the active substance is not achieved as expected, and various measures have been studied to improve this problem.

Therefore, the present invention provides a more homogeneous and stable cosmetic composition by changing the particle size distribution in a desired direction by solving the problems of the existing liposomes, and a cosmetic composition manufacturing method including liposome powder that can improve the feeling and adhesion of the cosmetic. I want to present it.

Embodiments of the present invention aim to provide a more homogeneous and stable cosmetic composition by achieving a change in physical properties such as particle size distribution without chemical additives.

In addition, it aims to improve product functions such as the feeling of use and adhesion of cosmetics.

In addition, it is intended to increase the absorption of active ingredients by changing the particle size distribution.

In addition, it is intended to improve productivity by increasing the production yield more usefully when preparing liposome particles through improved dispersibility.

According to an aspect of the invention, preparing a phospholipid solution; Forming liposome particles in the phospholipid solution through ultrasonic irradiation or a high-pressure microemulsifier; A method for preparing a cosmetic composition comprising liposome powder comprising a step of pulverizing the liposome particles through any one of spray drying, freeze drying or evaporation may be provided.

The method for preparing a cosmetic composition comprising liposome powder according to the present invention may further include the step of selectively passing the liposome particles through a membrane.

The phospholipid solution may contain ethanol.

The method for preparing a cosmetic composition comprising liposome powder according to the present invention further includes an active ingredient, and the active ingredient is a natural extract, oil, moisturizer, whitening agent, wrinkle improving agent, acne reliever, atopic reliever, sunscreen, hair growth agent, vitamin And its derivatives, amino acids or polypeptides, anti-inflammatory agents, female hormones, exfoliating agents, fungicides, placenta, allantoin, yeast extract, collagen, elastin, DHA, EPA, ceramide, fragrance, and at least any one selected from the group consisting of a mixture thereof It can be made one.

According to another aspect of the present invention, a cosmetic composition comprising the liposome powder prepared by the above-described manufacturing method may be provided.

Embodiments of the present invention can provide a more homogeneous and stable cosmetic composition by achieving changes in physical properties such as particle size distribution without chemical additives.

In addition, it is possible to achieve product functions such as the feeling of use and adhesion of cosmetics.

In addition, it is possible to increase the absorption power of the active ingredient by changing the particle size distribution.

In addition, when preparing liposome particles through improved dispersibility, productivity can be improved by increasing the production yield more usefully.

1 is a flow chart showing the manufacturing process of a cosmetic composition containing liposome powder according to an embodiment of the present invention
2 to 6 are images showing liposomes formed according to each condition using Cryo-TEM (Cryo-Transmission Electron Microscopy)
7 to 13 are graphs showing the results of measuring the particle size of liposomes formed according to each condition
14 to 20 are graphs showing the results of measuring the zeta potential of liposomes formed according to each condition
21 is a graph showing the result of measuring the color intensity of liposomes formed according to each condition
22 is a graph showing the results of analysis of Retinal content under 25°C conditions
23 is a graph showing the results of analysis of Retinal content under 45°C conditions
24 is a graph showing the results of evaluating the skin penetration rate of liposomes formed according to each condition.
25 to 29 are graphs evaluating the stability of liposomes formed according to each condition

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content may be thorough and complete, and the spirit of the present invention may be sufficiently conveyed to those skilled in the art. The same reference numbers throughout the specification indicate the same elements.

1 is a flow chart showing the manufacturing process of a cosmetic composition containing liposome powder according to an embodiment of the present invention.

Referring to FIG. 1, a method for preparing a cosmetic composition comprising liposome powder according to an embodiment of the present invention includes preparing a phospholipid solution (S10); Forming liposome particles in the phospholipid solution through ultrasonic irradiation or a high-pressure microemulsifier (S20); Powdering the liposome particles through any one of spray drying, lyophilization or evaporation (S40); may be included.

First, a method for preparing a cosmetic composition according to an embodiment of the present invention prepares a phospholipid solution (S10). In this embodiment, the phospholipid is yolk-derived phosphatidylcholine (Sigma, P3556), but is not limited thereto.

Specifically, a certain amount of powdery phospholipid is added to a washed test tube, and an organic solvent is added to dissolve it, and then the solvent is evaporated to dry the phospholipid to form a thin layer on the bottom of the test tube. And after adding a certain amount of the buffer solution, it is stirred with a vibrating mixer to disperse and dilute the phospholipid to form a phospholipid solution. At this time, the phospholipid solution may be composed of ethanol by applying ethanol as the buffer solution.

The specific composition of the phospholipid solution is as follows.

Table 1 shows the composition of the phospholipid solution when only simple liposomes are prepared.

Table 2 shows the composition of the phospholipid solution components when preparing a nanoemulsion.

Table 3 shows the composition of a phospholipid solution when preparing a nanoemulsion by applying Retinal.

Table 4 shows the composition of a phospholipid solution when preparing niosomes by applying Retinal.

Liposomal particles may be formed through ultrasonic irradiation or high-pressure microemulsifier in various phospholipid solutions configured as described above (S20). At this time, liposome formation can be performed by different conditions as shown in Table 5 below, and the size and volume of the liposome can be adjusted according to each condition.

A nanoemulsion (liposome) containing Retinal made using a high-pressure microemulsifier was passed through a membrane filter to obtain an emulsion (liposome) having particles of a certain size (S30).

The device and membrane filter used at this time were High Pressure Liposome Extruder (Jacketed Liposome Extruder with Track-Etched Membrane), Polycarbonate (PCTE) membrane filter (STERLITECH Corp., 0.1 Micron, 47 mm, 100/Pk), respectively.

As a process of powdering liposomes made with ultrasonic and high-pressure microemulsifiers, spray dryer, freeze drying, and evaporation were used. Each experimental device and conditions are shown in Table 6 below.

When preparing and pulverizing liposome particles, each sample was named according to the conditions for forming the sample.

Kinds Sample Unsaturated
(Lecithin) MF_LIPOSOME-1 MF_LIPOSOME-3 MF_LIPOSOME-5 saturation
(Hydrogenated Lecithin) MF_LIPOSOME-1 MF_LIPOSOME-3 MF_LIPOSOME-5 Unsaturated
(Lecithin) US_LIPOSOME-T10 US_LIPOSOME-T30 US_LIPOSOME-T50 saturation
(Hydrogenated Lecithin) US_LIPOSOME-T10 US_LIPOSOME-T30 US_LIPOSOME-T50 Unsaturated
(Lecithin) US_LIPOSOME-A30 US_LIPOSOME-A50 US_LIPOSOME-A70 saturation
(Hydrogenated Lecithin) US_LIPOSOME-A30 US_LIPOSOME-A50 US_LIPOSOME-A70

Condition Sample Normal

(Retinal applied) MF (unsaturated)_LIPOSOME-3 MF (saturated)_LIPOSOME-3 US (unsaturated)_LIPOSOME-T30 US (saturated)_LIPOSOME-T30 MF (saturation)_EMULSION-3 US (saturated)_EMULSION-T30 Freeze dryer

(Retinal applied) MF (unsaturated)_Retinal-PDR ^TM (FD) MF (saturated)_Retinal-PDR ^TM (FD) US (unsaturated)_Retinal-PDR ^TM (FD) US (saturated)_Retinal-PDR ^TM (FD) Evaporation

(Retinal applied) MF (unsaturated)_Retinal-PDR ^TM (EP) MF (saturated)_Retinal-PDR ^TM (EP) US (unsaturated)_Retinal-PDR ^TM (EP) US (saturated)_Retinal-PDR ^TM (EP) Spray dryer

(Retinal applied) MF (unsaturated)_Retinal-PDR ^TM (SD) MF (saturated)_Retinal-PDR ^TM (SD) US (unsaturated)_Retinal-PDR ^TM (SD) US (saturated)_Retinal-PDR ^TM (SD) Membrane

(Retinal applied) MF_NIOSOME MF_NIOSOME/F

When explaining the nomenclature of each sample shown in Tables 7 and 8, in the case of liposomes made using a high-pressure microemulsifier, it was named MF_LIPOSOME (EMULSION), and named as ‘MF_LIPOSOME-number of times’ according to the number of MF cycles. For example, MF_LIPOSOME-1 is a liposome made with a high-pressure microemulsifier, and the MF cycle refers to a sample that is once.

In the case of liposomes made by ultrasonic treatment, it was named US_LIPOSOME (EMULSION), and the nomenclature was changed according to the reaction time and intensity of the ultrasonic treatment. When the sonication reaction time was varied, the intensity was fixed at 50% and then the intensity was fixed at 50%, and when the reaction intensity was changed, the time was fixed at 30 minutes before proceeding. When the reaction time was changed, it was named “US_LIPOSOME-T time”, and when the intensity was changed, it was named “US_LIPOSOME-A century”.

For example, US_LIPOSOME-T30 is a liposome made by sonication, and refers to a sample in which the sonication reaction time is 30 minutes and the intensity is 50%. US_LIPOSOME-A30 refers to a sample with an ultrasonic treatment intensity of 30% and a reaction time of 30 minutes.

Freeze Drying, Evaporation, and Spray Drying were carried out under three MF conditions with stable size and zeta values, and US T30 sample conditions. They were named as'-Retinal-PDR ^™ (FD), (EP), (SD), respectively. In addition, samples that passed through the membrane filter were named with'/F' at the end.

For the liposome samples prepared according to each condition as described above, Cryo-TEM (Cryo-Transmission Electron Microscopy), particle size, and zeta potential analysis were performed.

2 to 6 are images showing liposomes formed according to each condition using Cryo-TEM (Cryo-Transmission Electron Microscopy). Specifically, the sample was kept at -176°C using liquid nitrogen to perform structural analysis in a frozen state.

Referring to each drawing, FIG. 2 is an image of analyzing normal liposomes. And Figure 3 is a freeze-drying, Figure 4 is an evaporation, Figure 5 is an image of the analysis of the liposome powdered by spray drying, Figure 6 is an image comparing the case of passing through the membrane filter and not passing through. .

Both the high-pressure microemulsifier and the sonication process formed liposomes well, and it can be seen that liposomes were formed more well when unsaturated lecithin was used than saturated lecithin. And the particle size became uniform when passing through the membrane filter.

Next, the average size of the formed liposome particles was measured. 7 to 13 are graphs showing the results of measuring the particle size of liposomes formed according to each condition.

In the experimental method, after preparing a 10% solution using purified water, particle size was measured using a laser light scattering system (Photal, ELS-Z, Japan).

Referring to each drawing, FIGS. 7 to 9 are graphs of the results of measuring the particle size of normal liposomes. And Figure 10 is a freeze-drying, Figure 11 is evaporation, Figure 12 is a graph showing the result of measuring the particle size of the liposome powdered by spray drying, Figure 13 is a case that passed through a membrane filter and not It is a graph comparing the particle size of the case.

The size was formed smaller when unsaturated lecithin was used than saturated lecithin, and in the case of ultrasonic liposomes, the size was the smallest when the reaction time was 30 minutes and the ultrasonic intensity was 50%.

On the other hand, the particle size increases when freeze-drying or evaporation is performed. At this time, the particle size is increased. Therefore, the particle size is measured after finely grinding with a mixer. And it can be seen that the particle size of the ultrasonic liposome is smaller than that of the high pressure microemulsifier liposome when the spray drying process is performed.

It can be seen that large-sized particles were filtered out when passing through the membrane.

Next is the measurement result of the zeta potential of the formed liposome. 14 to 20 are graphs showing the results of measuring the zeta potential of liposomes formed according to each condition.

In the experimental method, after preparing a 10% solution using purified water, the zeta potential was measured using a laser light scattering system (Photal, ELS-Z, Japan).

Referring to each drawing, FIGS. 14 to 16 are graphs of a result of measuring the zeta potential of a normal liposome. And FIG. 17 is a graph showing the results of measuring the zeta potential of liposomes powdered by lyophilization, FIG. 18 is evaporation, and FIG. 19 is a graph showing the results of measuring the zeta potential of the liposome powdered by spray drying. This is a graph comparing the zeta potential of the case.

Zeta potential can be seen as a stable state with a value of more than |40|mV. In the case of ultrasonic liposomes, it is believed that the reaction time of 30 minutes and the ultrasonic intensity of 30% have a high zeta potential value, and when freeze-drying, evaporation, and spray drying processes are performed, a higher zeta potential value than normal samples appears. It can be seen that it is relatively more stable.

In addition, when the membrane filter was used, there was little difference in the zeta potential value.

Next, stability evaluation was performed using a Chromameter. 21 is a graph showing the results of measuring the color intensity of liposomes formed according to each condition.

The experimental method was conducted for 1 month under a total of three conditions, such as light exposure, 40°C, and 25°C, using Color JS 555 (Color techno system, Japan). Unsaturated resin and Retinal applied samples were tested.

The color intensity was higher in MF_LIPOSOME and US_LIPOSOME than Retinal-PDR ^TM (FD), (EP) and (SD). ^{Comparing the color intensity, it can be seen that the color intensity of MF_LIPOSOME and US_LIPOSOME was higher than that of Retinal-PDR TM} (FD), (EP), and (SD) both when the light was illuminated and when the temperature was raised. .

When conditions change, MF_LIPOSOME and US_LIPOSOME have ^{more color change than Retinal-PDR TM} (FD), (EP), (SD), and through this, Retinal-PDR ^TM (FD), (EP), (SD) are MF_LIPOSOME, It can be seen that it is more stable than US_LIPOSOME.

Next, the change in the content of Retinal was analyzed. Specifically, the stability of the liposome was confirmed by analyzing the change in the 0.1% content of Retinal for 1 month. 22 is a graph showing the results of Retinal content analysis under 25°C condition, and FIG. 23 is a graph showing the results of Retinal content analysis under 45°C condition.

The experiment method is as follows.

-HPLC (Model 510 Waters USA)

-Detection by UV spectrophotometer(254nm)

-Capcell Pak C ₁₈ column(4.6 X 250 mm) shiseido

-Flow rate: 1.0 ml/min

-Mobile phase: 100% Methanol

-Unsaturated Lecithin, Retinal applied sample

As a result of analyzing the retinal content, Retinal-PDR™ (FD), Retinal-PDR™ (EP), and Retinal-PDR™ (SD) at both 25°C and 45°C have higher contents for 1 month than MF_LIPOSOME and US_LIPOSOME. Confirmed that there is. That is, since the reduction rate of Retinal content is low in Retinal-PDR™ (FD), Retinal-PDR™ (EP), and Retinal-PDR™ (SD), the stability is higher than that of MF_LIPOSOME and US_LIPOSOME.

Next, an experiment was conducted to evaluate the skin penetration effect. 24 is a graph showing the results of evaluating the skin penetration rate of liposomes formed according to each condition.

The experiment was carried out by attaching to a Franz-type diffusion cell (Transdermal Semi-Auto Diffusion Cell, LOGAN Instruments, USA) using an artificial skin Strat-M Membrane (Transdermal Diffusion Test Model).

After 50 mM phosphate buffer (pH 7.4, 0.1M NaCl) was added to the Receptor container (5 mL) of the Franz-type diffusion cell, the diffusion cell was mixed and dispersed at 32° C. and 600 rpm, and the sample containing the Retinal. Was put in a donor container.

It was absorbed and diffused according to a predetermined time, and it became 0.64 cm ² of the skin where absorption and diffusion occurred.

After the absorption and diffusion of the active ingredient is complete, wash the emulsion remaining on the skin without being absorbed with dried kimwipes or 10 mL of ethanol, and use a tip-type equalizer to grind the skin where the active ingredient is absorbed and diffused. The amount of Retinal absorbed inside was extracted using 4 mL of dichloromethane.

As a result of the experiment, as shown in FIG. 24, it can be seen that the skin penetration rate of Retinal-PDR™ (FD), Retinal-PDR™ (EP), and Retinal-PDR™ (SD) is higher than that of MF_LIPOSOME and US_LIPOSOME.

Next, the stability was evaluated using Turbiscan. 25 to 29 are graphs evaluating the stability of liposomes formed according to each condition.

The experimental method was conducted using Turbiscan (Turbiscan ^LAB , Formulaction, France) at 40°C, 4 hours, and 50 scans.

Referring to each drawing, FIG. 25 is MF_LIPOSOME-3, FIG. 26 is US_LIPOSOME-T30, FIG. 27 is Retinal-PDR™ (FD), FIG. 28 is Retinal-PDR™ (EP), FIG. 29 is Retinal-PDR™ ( SD) is a stability evaluation graph.

As shown in the graphs of each drawing, MF_LIPOSOME and US_LIPOSOME change over time, so creaming or coalescence/aggregation occurs, whereas Retinal-PDR™ (FD), Retinal-PDR™ (EP), Retinal-PDR™ (SD) can be confirmed to be in a stable state as the graph does not change significantly over time.

Overall, as seen above, it was confirmed that liposomes (emulsions) can be formed under various conditions such as high pressure microemulsifier, ultrasonic treatment, freeze drying, evaporation, and spray drying.

Comprehensive comparison of the particle size and the zeta potential value showed that unsaturated lecithin was more stable than saturated lecithin as a wall material, and when a membrane filter was applied, the particle size became more uniform.

As the number of MF increases, the particle size decreases, but there is no significant change when MF is performed 3 or more times. In the case of ultrasonic treatment, it was confirmed that, on average, a reaction time of 30 minutes and an ultrasonic intensity of 50% have a small particle size and a relatively stable zeta potential value.

Finally, a more stable powdered liposome form can be obtained through lyophilization, evaporation, and spray drying processes. In particular, Retinal-PDR™ samples that have undergone lyophilization, evaporation, and spray drying processes are more stable and , It was confirmed that the skin penetration rate was better.

Meanwhile, the cosmetic composition comprising the liposome powder according to the present invention may further include at least one active principle.

Specifically, the active ingredient is a natural extract, an oil, a moisturizing agent, a whitening agent, an anti-wrinkle agent, an acne reliever, an atopic reliever, a sunscreen agent, a hair restorer, a vitamin and its derivatives, an amino acid or a polypeptide, an anti-inflammatory agent, a female hormone agent, an exfoliating agent. , Fungicides, placenta, allantoin, yeast extract, collagen, elastin, DHA, EPA, ceramide, fragrance, and at least one selected from the group consisting of a mixture thereof.

At this time, the oil among the active ingredients may be made of at least one selected from the group consisting of fats and oils, waxes, hydrocarbon oils, fatty acid ester oils, alcohol oils, butters, silicone oils, ester oils, and mixtures thereof.

Specifically, the oils and fats include macadamia nut oil, olive oil, jojoba oil, almond oil, apricot seed oil, green tea oil, meadow foam oil, sun flower oil, argan oil, camellia oil, avocado oil, soybean oil, grape seed oil, castor oil, At least one selected from the group consisting of rice bran oil and mixtures thereof may be applied.

In addition, the wax may be made of at least one selected from the group consisting of carnauba wax, candelilly wax, jojoba oil, beeswax, lanolin, soy wax, rice wax, silicone wax, and mixtures thereof.

Meanwhile, as the hydrocarbon-based oil, at least one selected from the group consisting of liquid paraffin, paraffin, petrolatum, ceresin, micro crystal wax, squalane, and mixtures thereof may be used.

And the fatty acid ester-based oil may be made of at least one selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, isostearic acid, and mixtures thereof, and the alcohol-based oil includes cetyl alcohol, stearyl alcohol , Isostearyl alcohol, 2-octyldodecyl, 2-ethylhexanecetyl, diisostearyl malic acid, and at least one selected from the group consisting of mixtures thereof may be applied.

As the butter, at least one selected from the group consisting of shea butter, soy butter, mango butter, and mixtures thereof may be used.

And, as the silicone oil, at least one selected from the group consisting of dimethicones, cyclomethicones, silicone polymers, and mixtures thereof may be applied.

The ester-based oils are: cetylethylhexanoate, diglyceryltriisostearate, capric/caprylic triglyceride, glyceryltri2-ethylhexanoate, isononylisononanoate, ethylhexyl Sononanoate, ethylhexyl palmitate, isostearyl isostearate, neopentyl glycol dicaprate, neopentyl glycol diethylhexanoate, octyldodecylmyristate, pentaethryl tetraethylhexanoate, pentaethrylyl At least one selected from the group consisting of tetraisostearate, isotridensyl isononanoate, trimethylopropane triisostearate, squalane, and mixtures thereof may be used.

On the other hand, among the active ingredients, the moisturizing agent is a group consisting of sodium lactate, hydroproline, 2-phytolipo-5-carboxylic acid sodium, hyaluronic acid, sodium hyaluronate, ceramide, phytosterol, cholesterol, sitosterol, and mixtures thereof. It may be made of at least one selected from.

In addition, as the sunscreen agent among the active ingredients, at least one selected from the group consisting of a benzophenone derivative, a paraaminobenzoic acid derivative, a methoxycinnamic acid derivative, a salicylic acid derivative, and a mixture thereof may be applied.

Among the active ingredients, the whitening agent may be formed of at least one selected from the group consisting of arbutin and arbutin derivatives, kojic acid, vitamin C and vitamin C derivatives, and mixtures thereof.

Among the active ingredients, the hair growth agent may be composed of at least one or more of a blood circulation promoting agent or a local stimulating agent. Among these, the blood circulation promoting agent is Moongyeoncho extract, ceparatin, vitamin E and derivatives thereof, gamma oryzanol, proteoglycan, and their At least any one selected from the group consisting of mixtures may be used.

In addition, as the topical irritant among the hair restorer components, at least one selected from the group consisting of pepper tink, ginger tink, cantari tink, benzyl nicotinic acid, and mixtures thereof may be applied.

Among the active ingredients, the vitamin and its derivatives are not particularly limited, but vitamin A and its derivatives, vitamins B1, B2, B6, E and its derivatives, vitamins D, H, K, pantothenic acid and its derivatives, biotin, panthenolo and It may consist of at least one selected from the group consisting of a mixture thereof.

Here, among the active ingredients, the amino acids are cystine, cysteine, methionine, serine, leucine, tryptophan, amino acid extract, EGF (Epidermal Growth Factor), IGF (insulin-like growth factor), and It may be made of at least one selected from the group consisting of FGF (Fibroblast Growth Factor), kappa-peptide, and mixtures thereof.

In addition, among the active ingredients, the anti-inflammatory agent may consist of at least one selected from the group consisting of beta-glytilithinic acid, glytilytanic acid derivatives, aprene, aminocapronic acid, hydrocortisone, beta-glucan, lychorus, and mixtures thereof. have.

On the other hand, among the active ingredients, the acne treatment agent may consist of at least one selected from the group consisting of estradiol, estro, ethinylestrariol, tricrosan, azelic acid, and mixtures thereof.

In addition, among the active ingredients, the fungicide may be made of at least one selected from the group consisting of benzalkonium chloride, benzeltoniol chloride, harocalban, and mixtures thereof.

Among the active ingredients, the female hormone agent may be estrogen, and the estrogen may be at least one of estradiol, ethinyl estradiol, and isoflavone, which is a vegetable estrogen.

Of the active ingredients, the keratin dissolving agent may be made of at least one selected from the group consisting of sulfur, salicylic acid, aha, baha, lesorosine, and mixtures thereof.

On the other hand, as the natural extract among the active ingredients, extracts of animals and plants, marine animals, or seaweeds or ingredients obtained from them (functional ingredients having effects such as moisturizing, moisture, pores, troubles, whitening, elasticity moisturizing, anti-wrinkle, etc.) Can be applied.

Specifically, as the natural extracts, the natural extracts include pungyeonhwa, cheek beard, white flower, great king, licorice, aloe, chamomile, rosehip, hinokitiol, maroni, beta-carotene, ginseng extract, loofah, cucumber, seaweed extract, seaweed extract, young woman, self-made At least one selected from the group consisting of tree sap and mixtures thereof may be applied.

In addition, the natural extracts are Icelandic Notted Lac (Ascophyllum Nodosum Extract), Icelandic Yompus glacier water, deep sea microorganisms, Antarctic microorganisms, Morera fluviatilis extract, Arctic yeast, brown algae in the Arctic sea, sea parsley (Dulse) , piceaabies (nidiformis) extract, and it is possible to consist of at least one selected from the group consisting of mixtures thereof.

According to the method for preparing a cosmetic composition including liposome powder according to the embodiments of the present invention described so far, it is possible to provide a more homogeneous and stable cosmetic composition by achieving changes in physical properties such as particle size distribution without chemical additives, and Product functions such as adhesion can be improved.

In addition, it is possible to increase the absorption power of the active ingredient through a change in the particle size distribution, and when preparing liposome particles through improved dispersibility, productivity can be improved by more usefully increasing the production yield.

Although the above has been described with reference to an embodiment of the present invention, those skilled in the art may variously modify and change the present invention within the scope not departing from the spirit and scope of the present invention described in the claims below. You can do it. Therefore, if the modified implementation basically includes the elements of the claims of the present invention, all should be considered to be included in the technical scope of the present invention.

Claims (5)

Preparing a phospholipid solution containing unsaturated lecithin or saturated lecithin and an active ingredient;
Forming liposome particles by applying a high-pressure microemulsifier for 10 to 50 minutes, 30% to 70% intensity, or 1 to 5 cycles of the phospholipid solution;
Powdering the liposome particles through any one of spray drying, lyophilization, or evaporation; a method for preparing a cosmetic composition comprising liposome powder. The method of claim 1,
The method of manufacturing a cosmetic composition comprising a liposome powder further comprising; selectively passing the liposome particles through a membrane. The method of claim 1,
The method for producing a cosmetic composition comprising liposome powder, characterized in that the phospholipid solution contains ethanol. The method of claim 1,
The active ingredients are natural extracts, oils, moisturizers, whitening agents, anti-wrinkle agents, acne relieving agents, atopy relieving agents, sunscreens, hair growth agents, vitamins and derivatives thereof, amino acids or polypeptides, anti-inflammatory agents, female hormones, exfoliating agents, disinfectants, Placenta, allantoin, yeast extract, collagen, elastin, DHA, EPA, ceramide, at least one selected from the group consisting of fragrances and mixtures thereof. A cosmetic composition comprising liposome powder prepared by the manufacturing method according to any one of claims 1 to 4.

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