KR102196680B1 - Method for producing Aralia continentalis herbal drink - Google Patents

Method for producing Aralia continentalis herbal drink Download PDF

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KR102196680B1
KR102196680B1 KR1020180171995A KR20180171995A KR102196680B1 KR 102196680 B1 KR102196680 B1 KR 102196680B1 KR 1020180171995 A KR1020180171995 A KR 1020180171995A KR 20180171995 A KR20180171995 A KR 20180171995A KR 102196680 B1 KR102196680 B1 KR 102196680B1
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herbal
mixture
beverage
beverages
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KR20200081943A (en
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이상천
최유진
진희연
김수환
방지혜
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재단법인 임실치즈앤식품연구소
임실군
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/302Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/14Extraction
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/24Heat, thermal treatment

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

본 발명은 (1) 독활, 작약, 당귀, 백출, 귤피, 복령, 사삼, 계피, 맥아, 황기, 감초, 생강 및 스테비아를 혼합한 한약재 혼합물을 준비하는 단계; 및 (2) 상기 (1)단계의 준비한 한약재 혼합물에 물을 첨가한 후 추출하는 단계를 포함하여 제조하는 것을 특징으로 하는 독활 한방 음료의 제조방법 및 상기 방법으로 제조된 독활 한방 음료에 관한 것이다.The present invention comprises the steps of (1) preparing a mixture of herbal medicines in which dokhwa, peony, angelica, baekchul, orange peel, bokryeong, sasam, cinnamon, malt, astragalus, licorice, ginger and stevia are mixed; And (2) extracting after adding water to the herbal mixture prepared in step (1), and to a method for producing a herbal beverage prepared by the above method.

Description

독활 한방 음료의 제조방법{Method for producing Aralia continentalis herbal drink}Method for producing a herbal drink {Method for producing Aralia continentalis herbal drink}

본 발명은 독활, 작약, 당귀, 백출, 귤피, 복령, 사삼, 계피, 맥아, 황기, 감초, 생강 및 스테비아를 혼합한 한약재 혼합물을 이용하여 제조하는 것을 특징으로 하는 독활 한방 음료의 제조방법 및 상기 방법으로 제조된 독활 한방 음료에 관한 것이다.The present invention is a method for producing a herbal beverage of Dokhwal, characterized in that it is prepared using a mixture of herbal medicines mixed with Dokhwa, Peony, Angelica, Baekchul, Mandarin Peony, Bokryeong, Sasam, Cinnamon, Malt, Astragalus, Licorice, Ginger and Stevia. It relates to an herbal beverage prepared by the method.

독활은 쌍떡잎식물로 산형화목 두릅나무과의 여러해살이풀로 땅두릅이라고도 한다. 유사한 발음 때문에 땃두릅이라 불리기도 하나 땃두릅나무와는 다른 종이다. 산에서 자라며, 높이는 1.5 m이고 꽃을 제외한 전체에 털이 약간 있다. 잎은 어긋나고 길이 50~100 cm, 너비 3~20 cm이며 어릴 때에는 연한 갈색 털이 있다. 작은잎은 달걀 모양 또는 타원형이고 가장자리에 톱니가 있다. 잎 표면은 녹색이고 뒷면은 흰빛이 돌며 잎자루 밑부분 양쪽에 작은 떡잎이 있다. 꽃은 7~8월에 크고 연한 녹색으로 피고 원추꽃차례가 자라며 총상(總狀)으로 갈라진 가지 끝에 산형꽃차례로 달린 양성화이다. 열매는 장과로서 9~10월에 검게 익는다. 바람에 움직이지 않는다는 뜻으로 독활이라고 부른다. 이른봄 어린 순은 식용하며, 가을에 잎이 죽은 다음 흙을 덮어서 어린 순이 길게 자랄 수 있도록 한다. 뿌리는 약용하는데, 근육통·하반신·마비·두통·중풍의 반신불수 등에 많이 쓰인다.Dok-Hwal is a dicotyledonous plant and is a perennial plant of the umbel family Araliaceae. Because of its similar pronunciation, it is also called a firewood, but it is a different species from firewood. It grows in the mountains, the height is 1.5 m, and there are some hairs on the whole except for flowers. The leaves are alternate, 50-100 cm long, 3-20 cm wide, and have light brown hairs when they are young. Small leaves are egg-shaped or oval, and there are saw teeth on the edge. The surface of the leaf is green, the back side is white, and there are small cotyledons on both sides of the bottom of the petiole. Flowers bloom large and light green in July-August, conical inflorescences grow, and are bisexual flowers hung in umbelous inflorescences at the ends of branches split into racemes. The fruit is a berry and ripens black in September-October. It is called Dok-Hwal, meaning that it does not move in the wind. Early spring young sprouts are edible, and cover the soil after the leaves die in autumn so that young sprouts can grow longer. The root is medicated, and it is often used for muscle pain, lower body, paralysis, headache, and paraplegic dysentery.

독활의 국내 연구에서는 관절건강과 관련되는 면역기능 강화 분야에서 독활 물 추출물이 대식세포 면역 활성을 증진시키는 효능이 있다고 다수 보고되었으며, 독활은 항염증 유효성분 continentalic acid 화학구조 등 다수의 항염증 관련 보고가 있다. 또한, 독활의 국외연구로는 면역활성 증진이 독보적이고, 관절건강과 관련된 콜라겐 생합성을 증가시켰다는 보고가 있다. 우리나라 농가의 소득 대체 작목인 독활의 어린 새순을 식품에 적용하는 연구가 보고되고 있으며 성숙한 독활 잎은 가을이 되면 쓸모없이 그대로 버려지고 있어서 이를 채취하여 조사료원으로 이용한 연구가 보고되었다. 독활을 깻잎과 비교해보면 질기고 더 두꺼운 특성이 있으며, 독활을 이용하여 식품에 적용한 연구는 미미한 실정이다. 우리나라의 소득작물인 독활 잎은 2-5월에 비가림 및 노지재배 상품이 출하되는데 5월 중순 이후에는 온도상승으로 생식용으로 부적합한 상품이 다량 발생하여 저장식품인 김치를 제조하여 판매를 시도하고 있으나 저품질로 인해 판매의 어려움을 겪고 있어 독활 잎을 활용한 가공식품의 레시피 개발과 품질 개선이 필요한 실정이다.In domestic studies of Dok-Hwal, it has been reported that poison-half extract is effective in enhancing macrophage immune activity in the field of strengthening immune function related to joint health. There is. In addition, it is reported that the improvement of immune activity is unrivaled as an overseas study of Dok-Hwal, and that it increases collagen biosynthesis related to joint health. Studies have been reported on applying young sprouts of Dok-Hwal, which is an income replacement crop of Korean farms, to food, and as mature Dok-Hwal leaves are discarded as they are in autumn, studies have been reported that were collected and used as a forage source. Compared with sesame leaves, Dok-Hwal has a tougher and thicker character, and studies using Dok-Hwal to apply to food are insignificant. Dokhwa leaves, an income crop in Korea, are shipped in rain cover and field cultivation products in February-May. After mid-May, a large number of products unsuitable for reproductive use due to temperature increase occurred. However, due to the low quality, it is difficult to sell, so it is necessary to develop a recipe and improve the quality of processed foods using the leaves.

한국등록특허 제0931880호에는 침향, 조릿대, 원지 및 산조인을 함유하는 한방 음료의 제조방법이 개시되어 있으며, 한국등록특허 제0931879호에는 황금, 갈대뿌리 및 왕대를 포함하는 한방 음료가 개시되어 있으나, 본 발명의 독활 한방 음료의 제조방법과는 상이하다.Korean Patent No. 0931880 discloses a method of manufacturing an herbal beverage containing aloes, chorizo, raw paper, and sanjoin, and Korean Patent No. 0931879 discloses an herbal beverage containing gold, reed root, and royal stalk, It is different from the manufacturing method of the herbal beverage of the present invention.

본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명은 독활을 이용하여 음료 제조 시 품질 및 기호도가 우수한 음료를 제조하기 위해, 부재료 선정, 재료 전처리, 배합비 등의 제조조건을 최적화하여, 이취 및 이미가 없고 풍미 및 기호도가 우수한 독활 한방 음료를 개발하여 본 발명에 이르렀다.The present invention was conceived by the above requirements, and the present invention optimizes manufacturing conditions such as selection of subsidiary materials, material pretreatment, and mixing ratio in order to manufacture beverages with excellent quality and preference when manufacturing beverages by using poisoning. And it has come to the present invention by developing an herbal beverage that has no taste and has excellent flavor and preference.

상기 과제를 해결하기 위해, 본 발명은 (1) 독활, 작약, 당귀, 백출, 귤피, 복령, 사삼, 계피, 맥아, 황기, 감초, 생강 및 스테비아를 혼합한 한약재 혼합물을 준비하는 단계; 및 (2) 상기 (1)단계의 준비한 한약재 혼합물에 물을 첨가한 후 추출하는 단계를 포함하여 제조하는 것을 특징으로 하는 독활 한방 음료의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of: (1) preparing a mixture of herbal medicines in which dokhwa, peony, angelica, baekchul, orange peel, bokryeong, sasam, cinnamon, malt, astragalus, licorice, ginger and stevia are mixed; And (2) adding water to the herbal medicine mixture prepared in step (1) and then extracting it.

또한, 본 발명은 상기 방법으로 제조된 독활 한방 음료를 제공한다.In addition, the present invention provides a herbal beverage prepared by the above method.

본 발명의 독활 및 한약재를 이용한 음료는 재료 특유의 이취는 제거하고, 온화한 맛으로 인해 기호성이 증진되며, 항산화 활성이 높아 소비자들이 더욱 선호하는 음료를 제공할 수 있다.Beverages using toxic and herbal medicines of the present invention remove the characteristic odor of the ingredients, improve palatability due to a mild taste, and have high antioxidant activity, so that consumers can provide beverages that are more preferred by consumers.

도 1은 본 발명의 독활 한방 음료의 제조과정을 보여준다.
도 2는 독활 한방 음료의 저장 온도 및 기간에 따른 총 폴리페놀 함량 변화를 비교한 그래프이다.
도 3은 독활 한방 음료의 저장 온도 및 기간에 따른 DPPH 라디칼 소거능 변화를 비교한 그래프이다.
도 4는 독활 한방 음료의 저장 온도 및 기간에 따른 SOD 유사활성 변화를 비교한 그래프이다.
Figure 1 shows the manufacturing process of the Dokhwa herbal beverage of the present invention.
Figure 2 is a graph comparing the total polyphenol content change according to the storage temperature and duration of Dokhwa herbal beverages.
3 is a graph comparing changes in DPPH radical scavenging ability according to storage temperature and duration of toxic herbal beverages.
4 is a graph comparing the change in SOD-like activity according to the storage temperature and period of DokHwal herbal drinks.

본 발명의 목적을 달성하기 위하여, 본 발명은 In order to achieve the object of the present invention, the present invention

(1) 독활, 작약, 당귀, 백출, 귤피, 복령, 사삼, 계피, 맥아, 황기, 감초, 생강 및 스테비아를 혼합한 한약재 혼합물을 준비하는 단계; 및(1) preparing a mixture of herbal medicinal herbs mixed with Dokhwa, Peony, Angelica, Baekchul, Mandarin Peony, Bokryeong, Sasam, Cinnamon, Malt, Astragalus, Licorice, Ginger and Stevia; And

(2) 상기 (1)단계의 준비한 한약재 혼합물에 물을 첨가한 후 추출하는 단계를 포함하여 제조하는 것을 특징으로 하는 독활 한방 음료의 제조방법을 제공한다.(2) It provides a method for producing a herbal beverage, characterized in that it comprises the step of extracting after adding water to the herbal mixture prepared in step (1).

본 발명의 독활 한방 음료의 제조방법에서, 상기 (1)단계의 한약재 혼합물은 바람직하게는 한약재 혼합물 총 중량 기준으로, 독활 13~17 중량%, 작약 13~17 중량%, 당귀 6~9 중량%, 백출 5~7 중량%, 귤피 6~9 중량%, 복령 4~5 중량%, 사삼 5~7 중량%, 계피 2~4 중량%, 맥아 13~17 중량%, 황기 6~9 중량%, 감초 4~5 중량%, 생강 5~6 중량% 및 스테비아 2~4 중량%를 혼합하여 준비할 수 있으며, 더욱 바람직하게는 한약재 혼합물 총 중량 기준으로, 독활 15 중량%, 작약 15 중량%, 당귀 7.5 중량%, 백출 6 중량%, 귤피 7.5 중량%, 복령 4.5 중량%, 사삼 6 중량%, 계피 3 중량%, 맥아 15 중량%, 황기 7.5 중량%, 감초 4.5 중량%, 생강 5.5 중량% 및 스테비아 3 중량%를 혼합하여 준비할 수 있다. 상기와 같은 한약재 종류 및 배합비로 준비한 한약재 혼합물을 이용하여 음료를 제조하는 것이 독활과 한약재료들의 맛과 향이 잘 어우러지고 적절한 단맛과 깔끔한 맛으로 소비자들의 입맛에 맞는 음료를 제공할 수 있었다.In the method for producing a herbal beverage of the present invention, the herbal mixture of step (1) is preferably 13 to 17% by weight, 13 to 17% by weight of peony, 6 to 9% by weight of angelica, based on the total weight of the herbal mixture. , Baekchul 5-7 wt%, Tangerine peel 6-9 wt%, Bokryeong 4-5 wt%, Sasam 5-7 wt%, Cinnamon 2-4 wt%, Malt 13-17 wt%, Astragalus 6-9 wt%, Licorice 4-5% by weight, ginger 5-6% by weight, and 2-4% by weight of stevia can be mixed and prepared, more preferably, based on the total weight of the herbal mixture, 15% by weight, peony 15% by weight, Angelicae 7.5 wt%, Baekchul 6 wt%, Tangerine Peel 7.5 wt%, Bokryeong 4.5 wt%, Sasam 6 wt%, Cinnamon 3 wt%, Malt 15 wt%, Astragalus 7.5 wt%, Licorice 4.5 wt%, Ginger 5.5 wt% and Stevia It can be prepared by mixing 3% by weight. It is possible to provide a beverage that fits the tastes of consumers with an appropriate sweetness and clean taste, and the taste and aroma of the herbal medicines are well harmonized with the preparation of a beverage using the herbal medicine mixture prepared with the kind and mixing ratio of the herbal medicines as described above.

또한, 본 발명의 독활 한방 음료의 제조방법에서, 상기 (2)단계는 바람직하게는 한약재 혼합물에 혼합물 대비 물을 80~120배(v/w) 첨가한 후 90~110℃에서 1~3시간 동안 추출할 수 있으며, 더욱 바람직하게는 한약재 혼합물에 혼합물 대비 물을 100배(v/w) 첨가한 후 100℃에서 2시간 동안 추출할 수 있다. 상기와 같은 조건으로 추출하는 것이 각 재료들이 갖는 영양성분 및 풍미를 손상시키지 않으면서 재료들의 맛과 향이 잘 어우러지고 은은하게 느껴지는 한약재의 향과 맛으로 인해 기호도를 높일 수 있었다.In addition, in the manufacturing method of the herbal beverage of the present invention, the step (2) is preferably 80 to 120 times (v/w) of water compared to the mixture to the herbal medicine mixture, followed by 1 to 3 hours at 90 to 110°C. It can be extracted during, more preferably after adding 100 times (v/w) water compared to the mixture to the herbal medicine mixture, it can be extracted for 2 hours at 100 ℃. Extracting under the above-described conditions could enhance the preference due to the aroma and taste of the herbal medicine that the taste and aroma of the ingredients are well harmonized and subtle without damaging the nutritional components and flavors of each ingredient.

본 발명의 독활 한방 음료의 제조방법은, 보다 구체적으로는The manufacturing method of the herbal beverage of the present invention, more specifically

(1) 한약재 혼합물 총 중량 기준으로, 독활 13~17 중량%, 작약 13~17 중량%, 당귀 6~9 중량%, 백출 5~7 중량%, 귤피 6~9 중량%, 복령 4~5 중량%, 사삼 5~7 중량%, 계피 2~4 중량%, 맥아 13~17 중량%, 황기 6~9 중량%, 감초 4~5 중량%, 생강 5~6 중량% 및 스테비아 2~4 중량%를 혼합한 한약재 혼합물을 준비하는 단계; 및(1) Based on the total weight of the herbal mixture, Dok Hwa 13 to 17 wt%, Peony 13 to 17 wt%, Angelica 6 to 9 wt%, Baekchul 5 to 7 wt%, Tangerine Peel 6 to 9 wt%, Bokryeong 4 to 5 wt% %, 4 to 7% by weight, cinnamon 2 to 4% by weight, malt 13 to 17% by weight, Astragalus 6 to 9% by weight, licorice 4 to 5% by weight, ginger 5 to 6% by weight, and stevia 2 to 4% by weight Preparing a mixture of herbal medicine mixture; And

(2) 상기 (1)단계의 준비한 한약재 혼합물에 혼합물 대비 물을 80~120배(v/w) 첨가한 후 90~110℃에서 1~3시간 동안 추출하는 단계를 포함할 수 있으며,(2) After adding 80 to 120 times (v/w) water compared to the mixture to the herbal medicine mixture prepared in step (1) and then extracting at 90 to 110°C for 1 to 3 hours,

더욱 구체적으로는More specifically

(1) 한약재 혼합물 총 중량 기준으로, 독활 15 중량%, 작약 15 중량%, 당귀 7.5 중량%, 백출 6 중량%, 귤피 7.5 중량%, 복령 4.5 중량%, 사삼 6 중량%, 계피 3 중량%, 맥아 15 중량%, 황기 7.5 중량%, 감초 4.5 중량%, 생강 5.5 중량% 및 스테비아 3 중량%를 혼합한 한약재 혼합물을 준비하는 단계; 및(1) Based on the total weight of the herbal mixture, Dokhwa 15% by weight, Peony 15% by weight, Angelicae 7.5% by weight, Baekchul 6% by weight, Tangerine peel 7.5% by weight, Bokryeong 4.5% by weight, Sasam 6% by weight, Cinnamon 3% by weight, Preparing a herbal mixture of 15% by weight malt, 7.5% by weight Astragalus, 4.5% by weight licorice, 5.5% by weight ginger, and 3% by weight stevia; And

(2) 상기 (1)단계의 준비한 한약재 혼합물에 혼합물 대비 물을 100배(v/w) 첨가한 후 100℃에서 2시간 동안 추출하는 단계를 포함할 수 있다.(2) After adding 100 times (v/w) water compared to the mixture to the herbal medicine mixture prepared in step (1), extracting at 100°C for 2 hours may be included.

본 발명은 또한, 상기 방법으로 제조된 독활 한방 음료를 제공한다.The present invention also provides a herbal beverage prepared by the above method.

이하, 본 발명의 제조예 및 실시예를 들어 상세히 설명한다. 단, 하기 제조예 및 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 제조예 및 실시예에 한정되는 것은 아니다.Hereinafter, preparation examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples are only illustrative of the present invention, and the content of the present invention is not limited to the following Preparation Examples and Examples.

제조예Manufacturing example 1. One. 독활Poison 한방 음료 Herbal drink

(1) 한약재 혼합물 총 중량 기준으로, 독활 15 중량%, 작약 15 중량%, 당귀 7.5 중량%, 백출 6 중량%, 귤피 7.5 중량%, 복령 4.5 중량%, 사삼 6 중량%, 계피 3 중량%, 맥아 15 중량%, 황기 7.5 중량%, 감초 4.5 중량%, 생강 5.5 중량% 및 스테비아 3 중량%를 혼합한 한약재 혼합물을 준비하였다.(1) Based on the total weight of the herbal mixture, Dokhwa 15% by weight, Peony 15% by weight, Angelicae 7.5% by weight, Baekchul 6% by weight, Tangerine peel 7.5% by weight, Bokryeong 4.5% by weight, Sasam 6% by weight, Cinnamon 3% by weight, An herbal mixture was prepared in which 15% by weight of malt, 7.5% by weight of Astragalus, 4.5% by weight of licorice, 5.5% by weight of ginger, and 3% by weight of stevia were mixed.

(2) 상기 (1)단계의 준비한 한약재 혼합물에 혼합물 대비 물을 100배(v/w) 첨가한 후 100℃에서 2시간 동안 추출하였다.(2) After adding 100 times (v/w) water compared to the mixture to the herbal medicine mixture prepared in step (1), the mixture was extracted at 100°C for 2 hours.

독활 한방 음료 배합비(중량%)Mixing ratio of herbal drinks (% by weight) 재료material 함량(%) content(%) AA BB CC DD 독활 Poison 1818 1717 1515 1313 작약Peony 1515 1515 1515 1515 당귀Angelica 7.57.5 7.57.5 7.57.5 7.57.5 백출Baekchul 6.06.0 6.06.0 6.06.0 6.06.0 귤피Tangerine 7.57.5 7.57.5 7.57.5 7.57.5 복령Bokryeong 4.54.5 4.54.5 4.54.5 4.54.5 사삼Four three 6.06.0 6.06.0 6.06.0 6.06.0 계피cinnamon 3.03.0 3.03.0 3.03.0 3.03.0 맥아malt 1515 1515 1515 1515 황기Astragalus 7.57.5 7.57.5 7.57.5 7.57.5 감초licorice 4.54.5 4.54.5 4.54.5 4.54.5 생강ginger 5.55.5 5.55.5 5.55.5 5.55.5 스테비아 Stevia 00 1One 33 55 합계(g)Total (g) 100100 100100 100100 100100

상기 제조예 1의 방법으로 독활 한방 음료를 제조하되, (1)단계의 한약재 혼합물 준비 시 재료 배합비를 표 1의 A, B 및 D의 배합비로 준비한 한약재 혼합물을 이용하여 독활 한방 음료를 각각 제조한 후, 하기 실시예 1 내지 6과 같이 품질을 비교하였다.In the preparation of the herbal beverage, the herbal beverage was prepared by the method of Preparation Example 1, and the herbal herbal beverage was prepared by using the herbal mixture prepared in the composition ratio of A, B, and D in Table 1 when preparing the herbal mixture in step (1). Then, the quality was compared as in Examples 1 to 6 below.

실시예Example 1. One. 독활Poison 한방 음료의 관능검사 Sensory evaluation of herbal beverages

한방 음료의 기호도 검사를 실시하였다. 평가 항목으로는 색, 향, 맛, 전반적인 기호도로 하였고, 각 항목에 대하여 9점 척도법(1점: 아주 많이 싫음, 5점: 보통, 9점: 아주 많이 좋음)을 사용하여 평가하도록 하였다. 한방 음료는 제조하여 4℃ 및 25℃에 보관한 후 검사 직전에 꺼내어 일회용 종이컵에 약 30 mL씩 담아 입안을 헹굴 수 있도록 정수된 물을 함께 제공하였다. 기호도 조사 결과는 향과 전체적인 기호도에서 C 음료가 가장 높은 점수로 평가되었다.The preference of herbal beverages was also tested. The evaluation items were color, scent, taste, and overall preference, and each item was evaluated using a 9-point scale (1 point: very disliked, 5 points: average, 9 points: very good). Herbal beverages were prepared, stored at 4° C. and 25° C., taken out immediately before the test, and put about 30 mL each in a disposable paper cup, and purified water was provided together to rinse the mouth. As a result of the preference survey, C beverage was evaluated as the highest score in the aroma and overall preference.

독활 한방 음료의 관능검사Poison Sensory evaluation of herbal beverages 종류Kinds color incense flavor 전체적인 기호도Overall preference AA 6.456.45 5.925.92 6.546.54 8.008.00 BB 6.536.53 5.855.85 5.175.17 7.927.92 CC 6.516.51 6.166.16 5.775.77 8.848.84 DD 6.776.77 5.775.77 4.774.77 7.317.31

실시예Example 2. 2. 독활Poison 한방 음료의 pH 및 당도 PH and sugar content of herbal beverages

독활 한방 음료의 pH는 pH 미터(Orion star A211, Thermo scientific)를 이용하여 측정하였고, 당도 변화는 디지털 당도계(RA-250WE, ATAGO)로 측정하였다. pH 변화는 4℃ 및 25℃에서 저장기간이 경과함에 따라 큰 차이 없이 4.9~5.1 사이로 나타났다.The pH of Dokhwa herbal beverages was measured using a pH meter (Orion star A211, Thermo scientific), and the change in sugar content was measured with a digital sugar content meter (RA-250WE, ATAGO). The pH change was between 4.9 and 5.1 with no significant difference as the storage period elapsed at 4℃ and 25℃.

독활 한방 음료의 pHPH of herbal beverages pH pH 종류 Kinds AA BB CC D D 4℃4 0일0 days 4.974.97 4.994.99 4.984.98 5.005.00 5일5 days 4.934.93 4.914.91 4.924.92 4.954.95 10일10 days 5.035.03 5.025.02 5.005.00 4.904.90 15일15th 5.055.05 5.075.07 5.105.10 5.015.01 20일 20 days 5.015.01 5.015.01 4.904.90 5.005.00 25℃25 0일0 days 4.974.97 4.994.99 4.984.98 5.005.00 5일5 days 5.045.04 4.954.95 5.015.01 5.155.15 10일10 days 5.025.02 4.904.90 4.954.95 4.974.97 15일15th 5.015.01 4.984.98 4.934.93 4.854.85 20일20 days 4.974.97 4.954.95 4.914.91 4.904.90

당도는 A 음료는 스테비아 첨가 B, C 및 D 음료보다 당도 1 Brix 정도 낮게 나타내었고, 저장온도 및 기간에 따라 큰 변화량이 없었다.The sugar content of beverage A was about 1 Brix lower than that of beverages B, C and D with stevia, and there was no significant change depending on the storage temperature and duration.

독활 한방 음료의 당도The sweetness of the herbal drink BrixBrix 종류 Kinds AA BB CC D D 4℃4 0일0 days 2.802.80 3.403.40 3.503.50 3.603.60 5일5 days 2.802.80 3.103.10 3.603.60 3.703.70 10일10 days 2.702.70 3.003.00 3.403.40 3.503.50 15일15th 2.802.80 3.103.10 3.403.40 3.603.60 20일 20 days 2.802.80 3.103.10 3.403.40 3.503.50 25℃25 0일0 days 2.802.80 3.403.40 3.503.50 3.603.60 5일5 days 2.702.70 3.203.20 3.203.20 3.503.50 10일10 days 2.702.70 3.303.30 3.203.20 3.503.50 15일15th 2.802.80 3.103.10 3.303.30 3.403.40 20일20 days 2.802.80 3.203.20 3.303.30 3.403.40

실시예Example 3. 3. 독활Poison 한방 음료의 총 폴리페놀 Total Polyphenols in Herbal Beverages

총 폴리페놀은 Folin-Ciocaltus 발색법에 준해 분석하였다. 시료 1 mL에 증류수 7.5 mL를 가한 후 3분간 방치시킨 다음 Folin-Ciocalteu 시약 0.5 mL를 첨가하고 3.5% 무수탄산나트륨(sodium carbonate anhydrous) 1 mL를 넣고 1시간 동안 방치 후, 760 nm에서 흡광도를 측정하였다. 표준물질은 갈산을 사용하여 표준 검량 곡선을 작성 후 총 폴리페놀 함량을 정량하였다. 한방 음료의 저장기간이 경과함에 따라 총 폴리페놀 함량은 점차 증가하다가 일정한 수준을 나타내었으며 스테비아 첨가군들이 무첨가군에 비해 총 폴리페놀 함량이 높았지만 25℃에서는 무첨가군이 다소 높게 나타났다(도 2).Total polyphenols were analyzed according to the Folin-Ciocaltus color development method. After adding 7.5 mL of distilled water to 1 mL of the sample, it was allowed to stand for 3 minutes, 0.5 mL of Folin-Ciocalteu reagent was added, 1 mL of 3.5% sodium carbonate anhydrous was added, and allowed to stand for 1 hour, and the absorbance was measured at 760 nm. . As a standard, gallic acid was used to prepare a standard calibration curve and the total polyphenol content was quantified. As the storage period of herbal beverages elapsed, the total polyphenol content gradually increased and then showed a constant level. The total polyphenol content of the stevia-added group was higher than that of the non-added group, but the non-added group was slightly higher at 25°C (Fig. 2). .

실시예Example 4. 4. 독활Poison 한방 음료의 Herbal beverage DPPHDPPH 라디칼Radical 소거능Erasing ability

DPPH 자유 라디칼 소거활성은 Blois의 방법을 변형하여 측정하였다. 0.1 mM DPPH 용액 150 ㎕와 시료 50 ㎕를 혼합하여 30분간 암소에서 반응시킨 후 530 nm에서 흡광도를 측정하였다. 제조 당일 DPPH 라디칼 소거능은 스테비아(당성분) 첨가량이 많을수록 약간 높게 나타났으며 저장기간 및 온도에 따라 큰 변화가 없는 것으로 보아 영향을 미치지 않았다(도 3).DPPH free radical scavenging activity was measured by modifying the method of Blois. 150 µl of a 0.1 mM DPPH solution and 50 µl of a sample were mixed, reacted in the dark for 30 minutes, and the absorbance was measured at 530 nm. The DPPH radical scavenging ability on the day of manufacture was slightly higher as the amount of stevia (sugar component) added increased, and it was not affected by the fact that there was no significant change depending on the storage period and temperature (FIG. 3).

실시예Example 5. 5. 독활Poison 한방 음료의 SOD 유사 활성 SOD-like activity of herbal beverages

SOD 유사 활성은 시료 0.5 mL, Tris-HCl buffer(pH 8.5) 3 mL, 7.2 mM 피로갈롤 0.2 mL를 혼합하여 25℃에서 10분간 반응시킨 후, 1N HCl 1 mL로 반응을 정지시키고 420 nm에서 흡광도를 측정하였다. SOD 유사 활성 함량은 모든 음료가 저장기간에 따라 꾸준히 증가하였으며, 저장온도에는 큰 영향을 미치지 않는 것으로 나타났다(도 4).For SOD-like activity, 0.5 mL of a sample, 3 mL of Tris-HCl buffer (pH 8.5), and 0.2 mL of 7.2 mM pyrogallol were mixed and reacted for 10 minutes at 25°C, and the reaction was stopped with 1 mL of 1N HCl, and absorbance at 420 nm. Was measured. It was found that the SOD-like activity content steadily increased with the storage period of all beverages, and did not significantly affect the storage temperature (FIG. 4).

실시예Example 6. 6. 독활Poison 한방 음료의 미생물 오염도 Microbial contamination of herbal beverages

무균적으로 시료 1 mL를 채취하여 3M 페트리 필름에 분주한 후 일반세균 및 대장균군을 확인하기 위하여 37℃에서 24~48시간 배양한 후 미생물의 오염 여부를 확인하였다. 일반세균 및 대장균군은 저장기간 동안 음료에서 검출되지 않아 미생물학적으로 안전한 것으로 나타났다(표 5). 따라서 독활 첨가 한방 음료는 식품위생학적으로 안전한 것으로 판단되며 식품 공전상의 기준치(세균수: 1 mL당 100 이하, 대장균군: 음성)에 적합한 것으로 나타났다.1 mL of a sample was collected aseptically, dispensed on 3M Petri film, and cultured at 37° C. for 24 to 48 hours to check general bacteria and coliform groups, and then checked for contamination of microorganisms. General bacteria and coliforms were found to be microbiologically safe as they were not detected in beverages during the storage period (Table 5). Therefore, it was judged that herbal beverages added to poisonous medicine were safe for food hygiene and were found to be suitable for the standard value of food revolution (number of bacteria: less than 100 per mL, coliform group: negative).

독활 한방 음료의 미생물 오염도 측정Measurement of microbial contamination of herbal beverages 조건 Condition 일반세균
(Log cfu/g)
Common bacteria
(Log cfu/g)
대장균군
(Log cfu/g)
Coliform
(Log cfu/g)
온도(℃)Temperature(℃) 기간 (Days)Period (Days) 4℃4℃ 00 -- -- 55 -- -- 1010 -- -- 1515 -- -- 2020 -- -- 25℃25℃ 00 -- -- 55 -- -- 1010 -- -- 1515 -- -- 2020 -- --

실시예Example 7. 재료 종류에 따른 7. According to the type of material 독활Poison 한방 음료의 관능검사 Sensory evaluation of herbal beverages

상기 제조예 1의 방법으로 독활 한방 음료를 제조하되, (1)단계의 한약재 혼합물 준비 시 재료 배합비를 표 6의 E 및 F의 재료 및 배합비로 준비한 한약재 혼합물을 이용하여 독활 한방 음료를 각각 제조한 후, 실시예 1과 동일한 방법으로 관능검사를 실시하였다.In the preparation of the herbal beverage, the herbal beverage was prepared by the method of Preparation Example 1, and the herbal herbal beverage was prepared by using the herbal mixture prepared with the ingredients and mixing ratios of E and F in Table 6 when preparing the herbal mixture in step (1). Then, a sensory test was performed in the same manner as in Example 1.

독활 한방 음료 배합비(중량%)Mixing ratio of herbal drinks (% by weight) 재료material 함량(%) content(%) CC EE FF 독활 Poison 1515 1818 1515 작약Peony 1515 1818 1414 당귀Angelica 7.57.5 1010 66 백출Baekchul 6.06.0 77 55 귤피Tangerine 7.57.5 1010 66 복령Bokryeong 4.54.5 66 44 사삼Four three 6.06.0 -- 55 계피cinnamon 3.03.0 55 33 맥아malt 1515 -- 1414 황기Astragalus 7.57.5 1010 66 감초licorice 4.54.5 66 44 생강ginger 5.55.5 77 55 도라지Bellflower -- -- 44 갈근Prickly -- -- 33 천궁Sky -- -- 33 스테비아 Stevia 33 33 33 합계(g)Total (g) 100100 100100 100100

그 결과, C 음료와 같이, 독활, 작약, 당귀, 백출, 귤피, 복령, 사삼, 계피, 맥아, 황기, 감초 및 생강의 12가지 한약재만을 사용하여 음료를 제조하는 것이 향, 맛 및 전체적인 기호도가 가장 우수함을 확인할 수 있었다.As a result, like beverage C, making a beverage using only 12 herbal medicines such as Dokhwa, Peony, Angelica, Baekchul, Mandarin Peel, Bokryeong, Sasam, Cinnamon, Malt, Astragalus, Licorice, and Ginger The best was confirmed.

독활 한방 음료의 관능검사Sensory test of herbal drinks 종류Kinds color incense flavor 전체적인 기호도Overall preference CC 6.516.51 6.166.16 5.775.77 8.848.84 EE 6.506.50 5.685.68 5.225.22 6.926.92 FF 6.406.40 5.405.40 5.005.00 6.086.08

Claims (4)

(1) 한약재 혼합물 총 중량 기준으로, 독활 15 중량%, 작약 15 중량%, 당귀 7.5 중량%, 백출 6 중량%, 귤피 7.5 중량%, 복령 4.5 중량%, 사삼 6 중량%, 계피 3 중량%, 맥아 15 중량%, 황기 7.5 중량%, 감초 4.5 중량%, 생강 5.5 중량% 및 스테비아 3 중량%를 혼합한 한약재 혼합물을 준비하는 단계; 및
(2) 상기 (1)단계의 준비한 한약재 혼합물에 혼합물 대비 물을 100배(v/w) 첨가한 후 100℃에서 2시간 동안 추출하는 단계를 포함하여 제조하는 것을 특징으로 하는 독활 한방 음료의 제조방법.
(1) Based on the total weight of the herbal mixture, Dokhwa 15% by weight, Peony 15% by weight, Angelicae 7.5% by weight, Baekchul 6% by weight, Tangerine peel 7.5% by weight, Bokryeong 4.5% by weight, Sasam 6% by weight, Cinnamon 3% by weight, Preparing a herbal mixture of 15% by weight malt, 7.5% by weight astragalus, 4.5% by weight licorice, 5.5% by weight ginger, and 3% by weight stevia; And
(2) Preparation of an herbal beverage comprising the step of extracting for 2 hours at 100° C. after adding 100 times (v/w) water compared to the mixture to the herbal medicine mixture prepared in step (1) Way.
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