KR102422818B1 - Method for producing solid tea using powder of Aralia continentalis mixed extract having immune activity - Google Patents

Method for producing solid tea using powder of Aralia continentalis mixed extract having immune activity Download PDF

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KR102422818B1
KR102422818B1 KR1020200110146A KR20200110146A KR102422818B1 KR 102422818 B1 KR102422818 B1 KR 102422818B1 KR 1020200110146 A KR1020200110146 A KR 1020200110146A KR 20200110146 A KR20200110146 A KR 20200110146A KR 102422818 B1 KR102422818 B1 KR 102422818B1
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concentrate
ssanghwa
weight
poisonous
prepared
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최유진
정석근
송다혜
김남길
정성운
김영래
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재단법인 임실치즈앤식품연구소
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/22Drying or concentrating tea extract
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/30Further treatment of dried tea extract; Preparations produced thereby, e.g. instant tea
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • A23V2250/214Tea
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/10Drying, dehydrating
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/14Extraction
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/50Concentrating, enriching or enhancing in functional factors

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Plant Substances (AREA)
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Abstract

본 발명은 (1) 당귀, 천궁, 작약, 감초, 계피, 숙지황 및 황기의 쌍화재료에 주정을 첨가한 후 추출하고 농축하여 쌍화농축액을 제조하는 단계; (2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 혼합한 후 분무건조하여 쌍화농축액 분말을 제조하는 단계; (3) 독활, 도라지, 감초, 뽕나무열매, 건강, 구기자나무뿌리 및 모창출의 독활혼합재료에 물을 첨가하여 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계; (4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 혼합한 후 살균하고 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및 (5) 상기 (2)단계의 제조한 쌍화농축액 분말 및 상기 (4)단계의 제조한 독활 혼합추출분말과 설탕, 말토덱스트린, 호두 분쇄물, 대추 분쇄물, 생강엑기스 분말, 소금, 아몬드 및 전분을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법 및 상기 방법으로 제조된 고형차에 관한 것이다.The present invention comprises the steps of (1) extracting and concentrating Ssanghwa concentrate by adding alcohol to the Ssanghwa materials of Angelica keis, cheongung, peony, licorice, cinnamon, Sukjihwang and Hwanggi; (2) mixing dextrin with the Ssanghwa concentrate prepared in step (1), and then spray-drying to prepare a powder of the Ssanghwa concentrate; (3) preparing a poisonous mixture concentrate by adding water to the poisonous active mixture, bellflower, licorice, mulberry fruit, health, goji berry root, and seedling, followed by filtration and concentration; (4) mixing dextrin with the poisonous activity mixed concentrate prepared in step (3), followed by sterilization and hot air drying to prepare a poisonous activity mixed extract powder; and (5) the Ssanghwa concentrate powder prepared in step (2) and the mixed extract powder prepared in step (4) and sugar, maltodextrin, pulverized walnut, pulverized jujube, ginger extract powder, salt, almond and The present invention relates to a method for producing solid tea, comprising the step of mixing starch, and to a solid tea prepared by the method.

Description

면역 활성을 지니는 독활 혼합추출분말을 이용한 고형차의 제조방법{Method for producing solid tea using powder of Aralia continentalis mixed extract having immune activity}Method for producing solid tea using powder of Aralia continentalis mixed extract having immune activity

본 발명은 쌍화농축액 분말, 독활 혼합추출분말, 백설탕, 말토덱스트린, 호두 분쇄물, 대추 분쇄물, 생강엑기스 분말, 소금, 아몬드 및 전분을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법 및 상기 방법으로 제조된 고형차에 관한 것이다.The present invention is a solid tea comprising the step of mixing Ssanghwa concentrate powder, poison active mixed extract powder, white sugar, maltodextrin, pulverized walnut, jujube pulverized, ginger extract powder, salt, almond and starch. It relates to a manufacturing method and a solid tea manufactured by the method.

20세기 후반에 이르러 현대의 약으로 치료가 어려운 만성 대사성 질환이 급증하고 있으며, 특히 체질성(constitutional) 질환이나, 심인성(psychosomatic) 질환의 급증 및 합성의약품의 심각한 부작용이 알려지면서 기존의 의약품을 대체할 새로운 수단이 요구되고 있다. 전통적인 항생물질에 의한 치료의 가장 유망한 대체 수단으로 인체가 갖는 고유의 방어기구인 면역계의 활성화를 유도하는 면역 조절제의 이용이 제안되고 있다.In the second half of the 20th century, chronic metabolic diseases that are difficult to treat with modern drugs are rapidly increasing. A new means of doing this is required. The use of immune modulators that induce activation of the immune system, which is the body's own defense mechanism, has been proposed as the most promising alternative to treatment with traditional antibiotics.

면역계는 외부 위해 항원들의 침입이나 조직 손상을 야기하는 물질들이 신체에 침입하였을 때 이들로부터 신체를 보호하는 자기 방호 수단으로써 작용하고 림프구를 포함한 여러 가지 다양한 면역세포들에 의하여 다양한 면역반응들이 일어난다. 대식세포 활성화 지표 중의 하나인 NO는 사이토카인(cytokine)이나 미생물의 영향을 받아 대식세포에서 생성되는 반응질소 중간체로서 NOS(nitric oxide synthase)와 산소와 결합하여 L-아르기닌을 산화시켜 NO가 생성된다. 생성된 NO는 세포 내 감염을 일으키는 미생물과 암세포를 제어하는 것으로 보고되고 있고, NO가 필요 이상 생성되면 혈관확장, 염증반응에 의한 조직 손상, 돌연변이 등을 일으켜 생체 내 유해 작용을 나타내는 것으로도 알려져 있다. 따라서, NO는 이중적 특성을 나타내어, 약물 처리 시 세포독성을 유발하지 않는 농도에서 NO 생성을 촉진하면 면역기능을 증가시키는 것으로 판단할 수 있다.The immune system acts as a self-defense means to protect the body from intrusion of external harmful antigens or substances that cause tissue damage when they invade the body, and various immune responses occur by various immune cells including lymphocytes. NO, one of macrophage activation indicators, is a reactive nitrogen intermediate produced in macrophages under the influence of cytokines or microorganisms. It combines with NOS (nitric oxide synthase) and oxygen to oxidize L-arginine to produce NO. . The generated NO is reported to control microorganisms and cancer cells that cause intracellular infection, and when NO is generated more than necessary, it is also known to exhibit harmful effects in vivo by causing vasodilation, tissue damage due to inflammation, mutation, etc. . Therefore, NO exhibits dual characteristics, and it can be judged that when the production of NO is promoted at a concentration that does not induce cytotoxicity during drug treatment, the immune function is increased.

독활은 쌍떡잎식물로 산형화목 두릅나무과의 여러해살이풀로 땅두릅이라고도 하며, 바람에 움직이지 않는다는 뜻으로 독활이라고 부른다. 유사한 발음 때문에 땃두릅이라 불리기도 하나 땃두릅나무와는 다른 종이다. 산에서 자라며, 높이는 1.5 m이고 꽃을 제외한 전체에 털이 약간 있다. 잎은 어긋나고 길이 50~100 cm, 너비 3~20 cm이며 어릴 때에는 연한 갈색 털이 있다. 작은잎은 달걀모양 또는 타원형이고 가장자리에 톱니가 있다. 잎 표면은 녹색이고 뒷면은 흰빛이 돌며 잎자루 밑부분 양쪽에 작은 떡잎이 있다. 꽃은 7~8월에 크고 연한 녹색으로 피고 원추꽃차례가 자라며 총상(總狀)으로 갈라진 가지 끝에 산형꽃차례로 달린 양성화이다. 열매는 장과로서 9~10월에 검게 익는다. Poison bow is a dicotyledonous plant, and it is a perennial plant of the umbelliferae family Araliaceae. Although it is sometimes called a spruce tree because of its similar pronunciation, it is a different species from the spruce tree. It grows in the mountains, is 1.5 m high, and has some hairs all over except for flowers. Leaves are alternate phyllotaxis, 50-100 cm long and 3-20 cm wide, with light brown hairs when young. The small leaves are egg-shaped or oval and have serrated edges. The surface of the leaf is green and the back side is white, and there are small cotyledons on both sides of the lower part of the petiole. The flowers are large, light green, bloom in July-August, and a panicle grows, which is a bisexual flower that hangs in an umbel at the end of a branch divided into a raceme. The fruit is a berry and ripens black in September~October.

독활의 뿌리는 가을 또는 봄에 채취하여 햇볕에 말려두었다가 약용으로 쓰인다. 맛이 달면서 쓰고 성질은 평하며 독은 없다. 독활의 뿌리에는 디테르펜산 Ⅰ, Ⅱ, 정유(1~2%). 스테아린산(0.07%), 수지, 살리실산과 미량원소인 동, 망간, 니켈, 사포닌 등의 성분이 함유되어 있다. 독활은 스테로이드 호르몬의 복합물질을 함유하고 있어 신경 중추를 마비시키는 진통작용을 하며, 특히 신경통 치료에 그 효능이 인정되고 있다. 그 외에도 해열, 강심, 강장약, 근육통, 하반신마비, 중풍의 반신불수, 두통, 관절염, 감기, 부종, 치통, 류마티스, 피부가려움증, 만성기관지염 등에 약용한다.The roots of Dokhwal are collected in autumn or spring, dried in the sun, and used for medicinal purposes. The taste is sweet and bitter, and the nature is flat, and there is no poison. Diterpenic acids Ⅰ and Ⅱ, essential oil (1~2%) are contained in the roots of Poison Hal. It contains stearic acid (0.07%), resin, salicylic acid, and trace elements such as copper, manganese, nickel, and saponin. Toxin contains a compound of steroid hormones, and has an analgesic effect that paralyzes the nerve center, and its efficacy is particularly recognized for the treatment of neuralgia. In addition, it is used medicinally for antipyretic, strong heart, tonic, muscle pain, paralysis of the lower body, paraplegia, headache, arthritis, cold, edema, toothache, rheumatism, itchy skin, chronic bronchitis.

국내 연구에서는 관절건강과 관련되는 면역기능 강화 분야에서 독활 물 추출물이 대식세포 면역 활성을 증진시키는 효능이 있다고 다수 보고되었으며, 독활은 항염증 유효성분 continentalic acid 화학구조 등 다수의 항염증 관련 보고가 있다. 또한, 독활의 국외연구로는 면역활성 증진이 독보적이고, 관절건강과 관련된 콜라겐 생합성을 증가시켰다는 보고가 있다.In domestic studies, it has been reported that poisonous extracts are effective in enhancing macrophage immune activity in the field of strengthening immune function related to joint health. . In addition, there are reports that the enhancement of immune activity is unique in foreign studies of poison activity and that it increases collagen biosynthesis related to joint health.

한국등록특허 제1843499호에는 유자 및 쌍화차 분말을 이용한 티백차의 제조방법이 개시되어 있고, 한국공개특허 제2019-0021735호에는 독활, 두충 및 우슬로부터 생약 추출물의 제조방법이 개시되어 있으나, 본 발명의 독활 혼합추출분말을 이용한 고형차의 제조방법과는 상이하다.Korean Patent No. 1843499 discloses a method for producing tea bag tea using citron and Ssanghwa tea powder, and Korean Patent Publication No. 2019-0021735 discloses a method for preparing a herbal extract from poison ivy, duchung and mussel, but the present invention It is different from the manufacturing method of solid tea using the poison active mixed extract powder of

본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 독활을 이용하여 고형차 제조 시 품질 및 기호도가 우수한 고형차를 제조하기 위해, 부재료 선정, 재료 전처리, 배합비 등의 제조조건을 최적화하여, 이취 및 이미가 없고 풍미 및 기호도가 우수하면서 면역 활성을 지니는 고형차의 제조방법을 제공하는 데 있다.The present invention was devised in response to the above needs, and an object of the present invention is to manufacture a solid tea with excellent quality and preference when manufacturing a solid tea using poisonous activity, An object of the present invention is to provide a method for producing a solid tea having immune activity while having no off-flavor and taste, excellent flavor and palatability, by optimization.

상기 과제를 해결하기 위해, 본 발명은 (1) 당귀, 천궁, 작약, 감초, 계피, 숙지황 및 황기의 쌍화재료에 주정을 첨가한 후 추출하고 농축하여 쌍화농축액을 제조하는 단계; (2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 혼합한 후 분무건조하여 쌍화농축액 분말을 제조하는 단계; (3) 독활, 도라지, 감초, 뽕나무열매, 건강, 구기자나무뿌리 및 모창출의 독활혼합재료에 물을 첨가하여 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계; (4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 혼합한 후 살균하고 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및 (5) 상기 (2)단계의 제조한 쌍화농축액 분말 및 상기 (4)단계의 제조한 독활 혼합추출분말과 백설탕, 말토덱스트린, 호두 분쇄물, 대추 분쇄물, 생강엑기스 분말, 소금, 아몬드 및 전분을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of: (1) extracting and concentrating Ssanghwa concentrate after adding alcohol to Ssanghwa materials of Angelica keis, chrysanthemum, peony, licorice, cinnamon, Ssukjihwang and Hwanggi; (2) mixing dextrin with the Ssanghwa concentrate prepared in step (1), and then spray-drying to prepare a powder of the Ssanghwa concentrate; (3) preparing a poisonous mixture concentrate by adding water to the poisonous active mixture, bellflower, licorice, mulberry fruit, health, goji berry root, and seedling, followed by filtration and concentration; (4) mixing dextrin with the poisonous activity mixed concentrate prepared in step (3), followed by sterilization and hot air drying to prepare a poisonous activity mixed extract powder; and (5) the Ssanghwa concentrate powder prepared in step (2) and the mixed extract powder prepared in step (4), white sugar, maltodextrin, pulverized walnut, pulverized jujube, ginger extract powder, salt, almond and It provides a method for manufacturing solid tea, characterized in that it is prepared including the step of mixing starch.

또한, 본 발명은 상기 방법으로 제조된 고형차를 제공한다.In addition, the present invention provides a solid tea prepared by the above method.

본 발명의 독활 혼합추출분말은 면역 활성을 지니며, 상기 독활 혼합추출분말과 다양한 한약재 및 부재료를 이용한 고형차는 재료들이 잘 어우러지고 온화한 맛으로 인해 기호성이 증진되어 소비자들이 더욱 선호하는 고형차를 제공할 수 있다.The toxic active mixed extract powder of the present invention has immune activity, and the solid tea using the toxic active mixed extract powder and various herbal medicines and auxiliary materials harmonizes well with the ingredients and improves palatability due to the mild taste. can do.

도 1은 독활혼합농축액의 농도별 RAW 264.7 세포에 대한 독성을 비교한 그래프이다.
도 2는 독활혼합농축액의 농도별 NO 생성량을 비교한 그래프이다.
도 3은 독활혼합농축액의 농도별 IL-6 및 TNF-α 생성량을 비교한 그래프이다.
1 is a graph comparing the toxicity to RAW 264.7 cells by concentration of the poison-active mixture concentrate.
Figure 2 is a graph comparing the NO production amount by concentration of the poison-active mixture concentrate.
3 is a graph comparing the production of IL-6 and TNF-α according to the concentration of the poison-active mixture concentrate.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(1) 당귀, 천궁, 작약, 감초, 계피, 숙지황 및 황기의 쌍화재료에 주정을 첨가한 후 추출하고 농축하여 쌍화농축액을 제조하는 단계;(1) extracting and concentrating Ssanghwa concentrate by adding alcohol to the ingredients of Ssanghwa of Angelica, Chunkyung, Peony, Licorice, Cinnamon, Sukhumi Hwang and Hwanggi;

(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 혼합한 후 분무건조하여 쌍화농축액 분말을 제조하는 단계;(2) mixing dextrin with the Ssanghwa concentrate prepared in step (1), and then spray-drying to prepare a powder of the Ssanghwa concentrate;

(3) 독활, 도라지, 감초, 뽕나무열매, 건강, 구기자나무뿌리 및 모창출의 독활혼합재료에 물을 첨가하여 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계;(3) preparing a poisonous mixture concentrate by adding water to the poisonous active mixture, bellflower, licorice, mulberry fruit, health, goji berry root, and seedling, followed by filtration and concentration;

(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 혼합한 후 살균하고 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및(4) mixing dextrin with the poisonous activity mixed concentrate prepared in step (3), followed by sterilization and hot air drying to prepare a poisonous activity mixed extract powder; and

(5) 상기 (2)단계의 제조한 쌍화농축액 분말 및 상기 (4)단계의 제조한 독활 혼합추출분말과 설탕, 말토덱스트린, 호두 분쇄물, 대추 분쇄물, 생강엑기스 분말, 소금, 아몬드 및 전분을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법을 제공한다.(5) Ssanghwa concentrate powder prepared in step (2), mixed extract powder prepared in step (4), sugar, maltodextrin, pulverized walnut, pulverized jujube, ginger extract powder, salt, almond and starch It provides a method for manufacturing a solid tea, characterized in that it is prepared including the step of mixing.

본 발명의 고형차의 제조방법에서, 상기 (1)단계의 쌍화농축액은 바람직하게는 쌍화재료 총 중량 기준으로, 당귀 25~29 중량%, 천궁 21~25 중량%, 작약 7~9 중량%, 감초 9~11 중량%, 계피 15~17 중량%, 숙지황 7~9 중량% 및 황기 7~9 중량%의 쌍화재료에 45~55% 주정 8~10배(v/w) 첨가한 후 90~95℃에서 10~14시간 동안 추출하고 농축하여 제조할 수 있으며, 더욱 바람직하게는 쌍화재료 총 중량 기준으로, 당귀 27 중량%, 천궁 23 중량%, 작약 8 중량%, 감초 10 중량%, 계피 16 중량%, 숙지황 8 중량% 및 황기 8 중량%의 쌍화재료에 50% 주정 8~10배(v/w) 첨가한 후 90~95℃에서 12시간 동안 추출하고 농축하여 제조할 수 있다. 상기와 같은 재료 및 배합비로 쌍화재료를 혼합하는 것이 재료들의 향미와 맛이 조화를 이루고, 또한, 상기와 같은 조건으로 추출하는 것이 재료들의 맛과 향이 잘 어우러지고 깔끔한 맛을 내는 쌍화농축액으로 제조할 수 있었다.In the method for producing solid tea of the present invention, the Sssanghwa concentrate in step (1) is preferably 25 to 29% by weight of Angelica, 21 to 25% by weight of cheongung, 7 to 9% by weight of Peony, based on the total weight of Ssanghwa material, After adding 45~55% alcohol 8~10 times (v/w) to the Ssanghwa material of 9~11 wt% licorice, 15~17 wt% cinnamon, 7~9 wt% of Sukhumvitae, and 7~9 wt% of Astragalus, 90~ It can be prepared by extraction and concentration at 95° C. for 10 to 14 hours, and more preferably, based on the total weight of the Ssanghwa material, 27 wt% of angelica, 23 wt% of cheongung, 8 wt% of peony, 10 wt% of licorice, 16 wt% of cinnamon It can be prepared by adding 50% alcohol 8 to 10 times (v/w) to the Ssanghwa material of wt%, 8 wt% of Sukhumi sulfur, and 8 wt% of Astragalus, followed by extraction and concentration at 90-95°C for 12 hours. Mixing the Ssanghwa ingredients with the above ingredients and mixing ratio harmonizes the flavor and taste of the ingredients, and extracting under the same conditions as above can be prepared as a Ssanghwa concentrate that harmonizes the taste and aroma of the ingredients and gives a clean taste. could

또한, 본 발명의 고형차의 제조방법에서, 상기 (2)단계의 쌍화농축액 분말은 바람직하게는 쌍화농축액에 덱스트린을 2.5~3.5:6.5~7.5(v:w) 비율로 혼합한 후 180~190℃에서 분무건조하여 제조할 수 있으며, 더욱 바람직하게는 쌍화농축액에 덱스트린을 3:7(v:w) 비율로 혼합한 후 180~190℃에서 분무건조하여 제조할 수 있다. 상기와 같은 조건으로 분무건조하는 것이 저장성과 침강성 및 용해성도 우수하여, 고형차 제조에 적합한 쌍화농축액 분말로 제조할 수 있었다.In addition, in the method for producing a solid tea of the present invention, the powder of the Ssanghwa concentrate in step (2) is preferably 180 to 190 after mixing dextrin in the Ssanghwa concentrate in a ratio of 2.5 to 3.5:6.5 to 7.5 (v:w). It can be prepared by spray-drying at ℃, more preferably by mixing dextrin in the ssanghwa concentrate in a 3:7 (v:w) ratio and then spray-drying at 180-190 ℃. Spray-drying under the same conditions as described above was excellent in storability, settling properties, and solubility, and thus it was possible to prepare a Ssanghwa Concentrate powder suitable for the manufacture of solid tea.

또한, 본 발명의 고형차의 제조방법에서, 상기 (3)단계의 독활혼합농축액은 바람직하게는 독활혼합재료 총 중량 기준으로, 독활 20~24 중량%, 도라지 20~24 중량%, 감초 20~24 중량%, 뽕나무열매 10~12 중량%, 건강 10~12 중량%, 구기자나무뿌리 5~7 중량% 및 모창출 5~7 중량%의 독활혼합재료에 물을 7~9배(v/w) 첨가한 후 90~110℃에서 24~30시간 동안 추출한 후 여과 및 농축하여 제조할 수 있으며, 더욱 바람직하게는 독활혼합재료 총 중량 기준으로, 독활 22중량%, 도라지 22 중량%, 감초 22 중량%, 뽕나무열매 11 중량%, 건강 11 중량%, 구기자나무뿌리 6 중량% 및 모창출 6 중량%의 독활혼합재료에 물을 8배(v/w) 첨가한 후 100℃에서 27시간 동안 추출한 후 여과 및 농축하여 제조할 수 있다. 상기와 같은 조건으로 배합하여 추출하는 것이 각 재료들이 갖는 영양성분 및 풍미를 손상시키지 않으면서 재료들의 맛과 향이 잘 어우러지고 은은하게 느껴지는 한약재의 향과 맛으로 인해 기호도를 높일 수 있었다.In addition, in the method for producing solid tea of the present invention, the poisonous activity mixed concentrate of step (3) is preferably 20 to 24% by weight of poisonous activity, 20 to 24% by weight of bellflower, 20 to 24% by weight of licorice, based on the total weight of the poisonous active mixture. Water 7-9 times (v/w ) after addition, extraction at 90 to 110° C. for 24 to 30 hours, followed by filtration and concentration, and more preferably, based on the total weight of the poisonous active mixture, 22% by weight of poisonous activity, 22% by weight of bellflower, 22% by weight of licorice %, 11% by weight of mulberry fruit, 11% by weight of health, 6% by weight of Goji berry root, and 6% by weight of seedlings After adding water 8 times (v/w) to the poisonous mixture, extraction was performed at 100° C. for 27 hours. It can be prepared by filtration and concentration. Mixing and extracting under the above conditions did not damage the nutritional components and flavor of each material, and the taste and flavor of the ingredients harmonized well, and the taste and flavor of the herbal medicines were mildly felt.

또한, 본 발명의 고형차의 제조방법에서, 상기 (4)단계의 독활 혼합추출분말은 바람직하게는 독활혼합농축액에 덱스트린을 2.5~3.5:6.5~7.5(v:w) 비율로 혼합한 후 80~85℃에서 15~20분 동안 살균한 후 열풍건조하여 제조할 수 있으며, 더욱 바람직하게는 독활혼합농축액에 덱스트린을 3:7(v:w) 비율로 혼합한 후 80~85℃에서 15~20분 동안 살균한 후 열풍건조하여 제조할 수 있다. 상기와 같은 조건으로 혼합하여 살균처리 및 열풍건조하는 것이 한약재 특유의 쓴맛은 감소하고 부드러운 맛은 증진되어 기호도가 우수한 고형차 제조에 적합한 독활 혼합추출분말로 제조할 수 있었다. In addition, in the method for producing solid tea of the present invention, the poisonous activity mixed extract powder of step (4) is preferably mixed with dextrin in the poisonous activity mixed concentrate in a ratio of 2.5 to 3.5:6.5 to 7.5 (v:w), and then 80 It can be prepared by sterilizing at ~85℃ for 15~20 minutes and then drying with hot air, more preferably, after mixing dextrin in a poison-active mixture concentrate in a 3:7 (v:w) ratio, 15~ at 80~85℃ After sterilization for 20 minutes, it can be prepared by hot air drying. By mixing under the same conditions as described above, sterilization treatment and hot air drying reduce the characteristic bitter taste of herbal medicines and enhance the soft taste, thereby making it possible to prepare a poisonous active mixed extract powder suitable for manufacturing solid tea with excellent taste.

또한, 본 발명의 고형차의 제조방법에서, 상기 (5)단계는 바람직하게는 고형차 총 중량 기준으로, 쌍화농축액 분말 4~4.5 중량%, 독활 혼합추출분말 0.8~1.2 중량%, 설탕 63~65 중량%, 말토덱스트린 21~23 중량%, 호두 분쇄물 1.5~2.5 중량%, 대추 분쇄물 1.5~2.5 중량%, 생강엑기스 분말 0.4~0.6 중량%, 소금 0.4~0.6 중량%, 아몬드 1.5~2.5 중량% 및 전분 1.5~2.5 중량%를 혼합할 수 있으며, 더욱 바람직하게는 고형차 총 중량 기준으로, 쌍화농축액 분말 4.2 중량%, 독활 혼합추출분말 1 중량%, 백설탕 63.8 중량%, 말토덱스트린 22 중량%, 호두 분쇄물 2 중량%, 대추 분쇄물 2 중량%, 생강엑기스 분말 0.5 중량%, 소금 0.5 중량%, 아몬드 2 중량% 및 전분 2 중량%를 혼합하는 단계를 포함할 수 있다. 상기와 같은 재료 종류 및 배합비로 혼합하여 고형차를 제조하는 것이 독활과 한약재료들의 맛과 향이 잘 어우러지고 적절한 단맛과 깔끔한 맛으로 소비자들의 입맛에 맞는 고형차를 제공할 수 있었다.In addition, in the method for producing solid tea of the present invention, the step (5) is preferably based on the total weight of the solid tea, 4 to 4.5 wt% of Ssanghwa concentrate powder, 0.8 to 1.2 wt% of poison active mixed extract powder, 63 to sugar 65 wt%, maltodextrin 21-23 wt%, walnut pulverized 1.5-2.5 wt%, jujube pulverized 1.5-2.5 wt%, ginger extract powder 0.4-0.6 wt%, salt 0.4-0.6 wt%, almond 1.5-2.5 wt% Weight % and starch 1.5 to 2.5 wt % may be mixed, and more preferably, based on the total weight of solid tea, 4.2 wt % of Ssanghwa Concentrate Powder, 1 wt % of poison active mixed extract powder, 63.8 wt % of white sugar, 22 wt % of maltodextrin %, walnut pulverized 2% by weight, jujube pulverized 2% by weight, ginger extract powder 0.5% by weight, salt 0.5% by weight, almond 2% by weight and starch 2% by weight. By mixing the ingredients and mixing ratio as described above to prepare solid tea, it was possible to provide a solid tea that satisfies the taste of consumers with a suitable sweetness and clean taste, while harmonizing well with the taste and aroma of herbal ingredients.

본 발명의 고형차의 제조방법은, 보다 구체적으로는The method for producing a solid tea of the present invention is more specifically

(1) 쌍화재료 총 중량 기준으로, 당귀 25~29 중량%, 천궁 21~25 중량%, 작약 7~9 중량%, 감초 9~11 중량%, 계피 15~17 중량%, 숙지황 7~9 중량% 및 황기 7~9 중량%의 쌍화재료에 45~55% 주정 8~10배(v/w) 첨가한 후 90~95℃에서 10~14시간 동안 추출하고 농축하여 쌍화농축액을 제조하는 단계;(1) Based on the total weight of Ssanghwa ingredients, Angelica 25~29 wt%, Cheongung 21~25 wt%, Peony 7~9 wt%, Licorice 9~11 wt%, Cinnamon 15~17 wt%, Sukhumi Hwang 7~9 wt% % and 7 to 9% by weight of Ssanghwa material, 45 to 55% alcohol 8 to 10 times (v/w), followed by extraction and concentration at 90 to 95° C. for 10 to 14 hours to prepare a Ssanghwa concentrate;

(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 2.5~3.5:6.5~7.5(v:w) 비율로 혼합한 후 180~190℃에서 분무건조하여 쌍화농축액 분말을 제조하는 단계;(2) mixing dextrin with the Ssanghwa concentrate prepared in step (1) in a ratio of 2.5 to 3.5:6.5 to 7.5 (v:w) and spray-drying at 180 to 190° C. to prepare a powder of the Ssanghwa concentrate;

(3) 독활혼합재료 총 중량 기준으로, 독활 20~24 중량%, 도라지 20~24 중량%, 감초 20~24 중량%, 뽕나무열매 10~12 중량%, 건강 10~12 중량%, 구기자나무뿌리 5~7 중량% 및 모창출 5~7 중량%의 독활혼합재료에 물을 7~9배(v/w) 첨가한 후 90~110℃에서 24~30시간 동안 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계;(3) Based on the total weight of the poisonous mixture, 20~24% by weight of poisonous activity, 20~24% by weight of bellflower, 20~24% by weight of licorice, 10~12% by weight of mulberry fruit, 10~12% by weight of health, Goji berry root After adding 7-9 times (v/w) of water to 5-7 wt % and 5-7 wt % of hair-creating poisonous mixture, extraction is performed at 90-110° C. for 24-30 hours, followed by filtration and concentration for poison-active mixing preparing a concentrate;

(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 2.5~3.5:6.5~7.5(v:w) 비율로 혼합한 후 80~85℃에서 15~20분 동안 살균한 후 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및(4) After mixing dextrin in the ratio of 2.5 to 3.5:6.5 to 7.5 (v:w) in the poisonous activity mixed concentrate prepared in step (3), sterilize at 80 to 85° C. for 15 to 20 minutes, and then dry with hot air. Preparing a poison active mixed extract powder; and

(5) 고형차 총 중량 기준으로, 상기 (2)단계의 제조한 쌍화농축액 분말 4~4.5 중량% 및 상기 (4)단계의 제조한 독활 혼합추출분말 0.8~1.2 중량%와 백설탕 63~65 중량%, 말토덱스트린 21~23 중량%, 호두 분쇄물 1.5~2.5 중량%, 대추 분쇄물 1.5~2.5 중량%, 생강엑기스 분말 0.4~0.6 중량%, 소금 0.4~0.6 중량%, 아몬드 1.5~2.5 중량% 및 전분 1.5~2.5 중량%를 혼합하는 단계를 포함할 수 있으며,(5) Based on the total weight of the solid tea, 4 to 4.5 wt% of the Ssanghwa Concentrate powder prepared in step (2) and 0.8 to 1.2 wt% of the poison active mixed extract powder prepared in the step (4) and 63 to 65 weight of white sugar %, maltodextrin 21-23 wt%, walnut pulverized 1.5-2.5 wt%, jujube pulverized 1.5-2.5 wt%, ginger extract powder 0.4-0.6 wt%, salt 0.4-0.6 wt%, almond 1.5-2.5 wt% And it may include a step of mixing 1.5 to 2.5% by weight of starch,

더욱 구체적으로는more specifically

(1) 쌍화재료 총 중량 기준으로, 당귀 27 중량%, 천궁 23 중량%, 작약 8 중량%, 감초 10 중량%, 계피 16 중량%, 숙지황 8 중량% 및 황기 8 중량%의 쌍화재료에 50% 주정 8~10배(v/w) 첨가한 후 90~95℃에서 12시간 동안 추출하고 농축하여 쌍화농축액을 제조하는 단계;(1) Based on the total weight of Ssanghwa ingredients, 50% of Ssanghwa ingredients of 27 wt% Angelica, 23 wt% cheongung, 8 wt% peony, 10 wt% licorice, 16 wt% cinnamon, 8 wt% Ssukjihwang and 8 wt% Hwanggi After adding 8 to 10 times (v/w) alcohol, extracting and concentrating at 90 to 95° C. for 12 hours to prepare a Ssanghwa concentrate;

(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 3:7(v:w) 비율로 혼합한 후 180~190℃에서 분무건조하여 쌍화농축액 분말을 제조하는 단계;(2) mixing dextrin with the Ssanghwa concentrate prepared in step (1) at a ratio of 3:7 (v:w) and spray-drying at 180 to 190° C. to prepare a powder of the Ssanghwa concentrate;

(3) 독활혼합재료 총 중량 기준으로, 독활 22 중량%, 도라지 22 중량%, 감초 22 중량%, 뽕나무열매 11 중량%, 건강 11 중량%, 구기자나무뿌리 6 중량% 및 모창출 6 중량%의 독활혼합재료에 물을 8배(v/w) 첨가한 후 100℃에서 27시간 동안 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계;(3) Based on the total weight of the poisonous mixture, poisonous activity 22% by weight, bellflower 22% by weight, licorice 22% by weight, mulberry fruit 11% by weight, health 11% by weight, Goji berry root 6% by weight, and seedling 6% by weight After adding water 8 times (v/w) to the poisonous activity mixture, extracting at 100° C. for 27 hours, filtration and concentration to prepare a poisonous activity mixture concentrate;

(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 3:7(v:w) 비율로 혼합한 후 80~85℃에서 15~20분 동안 살균한 후 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및(4) After mixing dextrin with the poisonous activity mixed concentrate prepared in step (3) at a ratio of 3:7 (v:w), sterilization at 80~85℃ for 15~20 minutes, hot air drying, poisonous activity mixed extract powder preparing a; and

(5) 고형차 총 중량 기준으로, 상기 (2)단계의 제조한 쌍화농축액 분말 4.2 중량% 및 상기 (4)단계의 제조한 독활 혼합추출분말 1 중량%와 백설탕 63.8 중량%, 말토덱스트린 22 중량%, 호두 분쇄물 2 중량%, 대추 분쇄물 2 중량%, 생강엑기스 분말 0.5 중량%, 소금 0.5 중량%, 아몬드 2 중량% 및 전분 2 중량%를 혼합하는 단계를 포함할 수 있다.(5) Based on the total weight of the solid tea, 4.2 wt% of the Ssanghwa concentrate powder prepared in step (2) and 1 wt% of the poisonous active mixed extract powder prepared in step (4), 63.8 wt% of white sugar, and 22 wt% of maltodextrin %, walnut pulverized 2% by weight, jujube pulverized 2% by weight, ginger extract powder 0.5% by weight, salt 0.5% by weight, almond 2% by weight and starch 2% by weight.

본 발명은 또한, 상기 방법으로 제조된 고형차를 제공한다.The present invention also provides a solid tea prepared by the above method.

이하, 본 발명의 제조예 및 실시예를 들어 상세히 설명한다. 단, 하기 제조예 및 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 제조예 및 실시예에 한정되는 것은 아니다.Hereinafter, production examples and examples of the present invention will be described in detail. However, the following Preparation Examples and Examples only illustrate the present invention, the content of the present invention is not limited to the following Preparation Examples and Examples.

제조예 1. 고형차 제조Preparation Example 1. Preparation of solid tea

(1) 당귀 27 중량%, 천궁 23 중량%, 작약 8 중량%, 감초 10 중량%, 계피 16 중량%, 숙지황 8 중량% 및 황기 8 중량%의 쌍화재료에 쌍화재료 대비 50%(v/v) 주정 8~10배(v/w) 첨가한 후 90~95℃에서 12시간 동안 추출하고 농축하여 쌍화농축액을 제조하였다.(1) Angelica 27 wt%, chrysanthemum 23 wt%, peony 8 wt%, licorice root 10 wt%, cinnamon 16 wt%, Ssukjihwang 8 wt% and Hwanggi 8 wt% 50% (v/v) ) 8 to 10 times (v/w) of alcohol was added, followed by extraction and concentration at 90 to 95° C. for 12 hours to prepare a Ssanghwa Concentrate.

(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 3:7(v:w) 비율로 혼합한 후 180~190℃에서 분무건조하여 쌍화농축액 분말을 제조하였다.(2) After mixing dextrin with the Ssanghwa concentrate prepared in step (1) at a ratio of 3:7 (v:w), spray-drying at 180 to 190° C. to prepare a powder of the Ssanghwa concentrate.

(3) 독활 뿌리 22 중량%, 도라지 22 중량%, 감초 22 중량%, 뽕나무열매 11 중량%, 건강 11 중량%, 구기자나무뿌리 6 중량% 및 모창출 6 중량%의 독활혼합재료에 독활혼합재료 대비 정제수를 8배(v/w) 첨가한 후 100℃에서 27시간 동안 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하였다.(3) Poisonous active mixed material of 22 wt% of poisonous root, 22 wt% of bellflower, 22 wt% of licorice, 11 wt% of mulberry fruit, 11 wt% of health, 6 wt% of Goji berry root and 6 wt% of seedling After adding 8 times (v/w) of contrast purified water, extraction was performed at 100° C. for 27 hours, followed by filtration and concentration to prepare a poison-active mixture concentrate.

(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 3:7(v:w) 중량비율로 혼합한 후 80~85℃에서 15~20분 동안 살균한 후 열풍건조하여 독활 혼합추출분말을 제조하였다.(4) After mixing dextrin with the poisonous activity mixed concentrate prepared in step (3) at a weight ratio of 3:7 (v:w), sterilization at 80~85℃ for 15~20 minutes, hot air drying to extract poisonous activity A powder was prepared.

(5) 상기 (2)단계의 제조한 쌍화농축액 분말 4.2 중량% 및 상기 (4)단계의 제조한 독활 혼합추출분말 1 중량%와 백설탕 63.8 중량%, 말토덱스트린 22 중량%, 호두 분쇄물 2 중량%, 대추 분쇄물 2 중량%, 생강엑기스 분말 0.5 중량%, 소금 0.5 중량%, 아몬드 분쇄물 2 중량%, 전분 2 중량%를 혼합하여 고형차를 제조하였다.(5) 4.2% by weight of the Ssanghwa concentrate powder prepared in step (2), 1% by weight of the mixed extract powder prepared in step (4), 63.8% by weight of white sugar, 22% by weight of maltodextrin, 2% by weight of pulverized walnut %, 2% by weight of crushed jujube, 0.5% by weight of ginger extract powder, 0.5% by weight of salt, 2% by weight of crushed almond, and 2% by weight of starch were mixed to prepare a solid tea.

실시예 1. 독활혼합농축액의 마우스 유래 대식세포주 RAW264.7에 대한 세포독성 평가Example 1. Cytotoxicity evaluation of the toxic activity mixture concentrate against the mouse-derived macrophage line RAW264.7

독활혼합농축액의 마우스 유래 대식세포주 RAW264.7에 대해 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)법을 이용하여 세포독성을 평가하였다. RAW264.7 세포를 Fetal bovine serum(FBS) 10%가 함유된 DMEM 배지 500 ㎕에 24 well plate를 기준으로 3×105 cells으로 세포를 분주하였다. 24시간 후 FBS가 없는 DMEM 배지로 바뀌준 후, 독활혼합농축액을 각 농도별로 1%(v/v), 24시간 동안 처리하였다. 독활혼합농축액 반응 24시간 후 8 mg/ml MTT 시약을 50 ㎕ 처리 후 4시간 이상 반응시켰다. MTT 반응 종료 후 웰(well)의 상층액을 12,000 rpm, 10분 원심분리 후 웰 바닥면에 남아있는 세포에 DMSO 600 ㎕를 넣어 생성된 불용성 포르마잔(insoluble formazan)을 용해시켜 플레이트 리더(plate reader)에서 540 nm로 측정하였다.The cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against the mouse-derived macrophage cell line RAW264.7 of the toxic activity mixture. RAW264.7 cells were seeded into 3×10 5 cells in 500 μl of DMEM medium containing 10% Fetal bovine serum (FBS) based on a 24 well plate. After 24 hours, it was changed to DMEM medium without FBS, and the toxic activity mixture was treated with 1% (v/v) for each concentration for 24 hours. After 24 hours of reaction with the toxic activity mixture, 50 μl of 8 mg/ml MTT reagent was treated and reacted for at least 4 hours. After completion of the MTT reaction, the supernatant of the well was centrifuged at 12,000 rpm for 10 minutes. Then, 600 μl of DMSO was added to the cells remaining on the bottom of the well to dissolve the insoluble formazan, followed by a plate reader. ) at 540 nm.

그 결과, 독활혼합농축액의 RAW264.7 세포에 대한 독성은 최고농도인 100 mg/ml까지 세포독성을 보이지 않았다(도 1). 상기 결과로 독활혼합농축액에 대한 세포 및 in vitro 실험에서 처리농도의 기준점을 확립하였다.As a result, toxicity to RAW264.7 cells of the toxic activity mixture did not show cytotoxicity up to the highest concentration of 100 mg/ml (FIG. 1). As a result, the reference point of the treatment concentration was established in the cell and in vitro experiments for the toxic activity mixture concentrate.

실시예 2. 독활혼합농축액의 면역증강 기능성(nitric oxide) 평가Example 2. Evaluation of immune enhancing function (nitric oxide) of toxic active mixture concentrate

독활혼합농축액을 MTT법에 의해 독성이 없는 농도를 선택하여 면역증강의 지표인 NO(nitric oxide)를 평가하였다. RAW264.7 세포를 FBS(Fetal bovine serum) 10%가 함유된 DMEM 배지 500 ㎕에 24 well plate 기준으로 3×105 cells으로 세포를 분주하였다. 24시간 후 FBS가 없는 DMEM 배지로 바뀌준 후, 독활혼합농축액을 각 농도별로 1%(v/v), 48시간 처리하였다. 독활혼합농축액을 48시간 반응 후 웰의 상층액을 12,000 rpm, 15분 동안 원심분리하였다. 96 웰 플레이트에 그리스 시약 A와 B 1:1 혼합액 100 ㎕와 상층액 100 ㎕를 각각 넣어준 후 상온에서 5분간 발색하였다. 상기 발색이 끝난 플레이트를 마이크로 플레이트 리더(Micro plate reader)에서 540 nm의 파장으로 측정하였다.The concentration of non-toxic mixture was selected by MTT method to evaluate NO (nitric oxide), an indicator of immunity enhancement. RAW264.7 cells were seeded in 500 μl of DMEM medium containing 10% Fetal bovine serum (FBS) at 3×10 5 cells based on a 24 well plate. After 24 hours, the medium was changed to DMEM without FBS, and the toxic activity mixture was treated with 1% (v/v) for each concentration for 48 hours. After 48 hours of reaction with the toxic activity mixture, the supernatant of the well was centrifuged at 12,000 rpm for 15 minutes. In a 96-well plate, 100 μl of a 1:1 mixture of grease reagents A and B and 100 μl of the supernatant were each added, followed by color development at room temperature for 5 minutes. The color-developed plate was measured with a wavelength of 540 nm in a micro plate reader.

그 결과, 도 2에 나타난 바와 같이 독활혼합농축액 1 mg/ml에서 면역증강의 지표인 NO(nitric oxide) 생성이 약 26% 증가되었다.As a result, as shown in FIG. 2, the production of nitric oxide (NO), which is an indicator of immune enhancement, was increased by about 26% at 1 mg/ml of the poison-active mixture concentrate.

실시예 3. 독활혼합농축액의 면역증강 기능성(IL-6, TNF-α) 평가Example 3. Immune enhancing function (IL-6, TNF-α) evaluation of the poisonous activity mixture concentrate

독활혼합농축액의 상층액에 존재하는 면역증강지표 사이토카인(IL-6, TNF-α)의 양을 확인하기 위해 Sandwich ELISA(BD Opti EIA ELISA kit)를 이용하여 측정(405 nm)하였다.To determine the amount of immune-enhancing indicator cytokines (IL-6, TNF-α) present in the supernatant of the poison-active mixture concentrate, it was measured (405 nm) using Sandwich ELISA (BD Opti EIA ELISA kit).

독활혼합농축액의 사이토카인 생성에 따른 면역증강 기능성 평가를 한 결과, IL-6의 생성량은 100 mg/ml에서 blank군 보다 약 2배의 생성량을 나타내었으며, 종양 괴사 인자인 TNF-α의 생성량은 10 mg/ml과 100 mg/ml에서 blank군 보다 약 6배의 생성량을 보였다(도 3).As a result of evaluating the immune enhancement function according to the cytokine production of the poison-active mixture, the production amount of IL-6 was about twice that of the blank group at 100 mg/ml, and the production amount of TNF-α, a tumor necrosis factor, was At 10 mg/ml and 100 mg/ml, the production amount was about 6 times higher than that of the blank group (FIG. 3).

실시예 4. 쌍화농축액 및 독활혼합농축액의 배합비에 따른 고형차의 관능검사Example 4. Sensory test of solid difference according to the mixing ratio of Ssanghwa concentrate and poison active mixed concentrate

제조예 1의 (1)단계의 쌍화농축액과 (3)단계의 독활혼합농축액의 재료 종류 및 배합비를 달리하여 제조한 고형차(비교예 1 내지 3)를 가지고 관능검사를 실시하였다. 관능검사는 고형차를 뜨거운 물에 성인 남녀 50명을 대상으로 색, 향, 맛 및 전체적인 기호도를 구분하여 1점 매우 나쁘다, 4점 보통, 7점 매우 좋음으로 나타나는 7점 기호척도법을 사용하였다.A sensory test was performed with solid differences (Comparative Examples 1 to 3) prepared by different material types and mixing ratios of the Ssanghwa concentrate of step (1) and the poison-active mixture concentrate of step (3) of Preparation Example 1 (Comparative Examples 1 to 3). For the sensory test, 50 adult males and females in hot water were subjected to a 7-point preference scale method, in which 1 point was very bad, 4 points were average, and 7 points were very good by classifying color, aroma, taste, and overall preference.

재료 배합비(중량%)Material mixing ratio (wt%) 재료 종류material type 제조예 1Preparation Example 1 비교예 1Comparative Example 1 비교예 2Comparative Example 2 비교예 3Comparative Example 3 쌍화농축액Ssanghwa Concentrate 당귀donkey 2727 2727 2222 3131 천궁celestial palace 2323 2323 2222 1818 작약peony 88 88 66 1111 감초licorice 1010 1010 88 1212 계피cinnamon 1616 1616 1010 1818 숙지황Suk Sook-hwang 88 -- 88 55 황기Astragalus 88 1616 88 55 대추Jujube -- -- 88 -- 갈근craving -- -- 88 -- 독활혼합농축액Toxic Activity Mixture Concentrate 독활solitary 2222 2525 1818 2626 도라지balloon flower 2222 2525 1818 1616 감초licorice 2222 2525 1818 2626 뽕나무열매mulberry fruit 1111 -- 1010 66 건강health 1111 2525 1010 66 구기자나무뿌리Goji berry root 66 -- 1010 44 모창출mother creation 66 -- 66 1616 녹용velvet -- -- 55 -- 인삼Ginseng -- -- 55 --

그 결과, 하기 표 2에 비교한 바와 같이, 제조예 1의 고형차가 가장 높은 점수를 나타내었고, 비교예 2의 고형차가 전반적으로 가장 낮은 점수를 나타내었다. 따라서, 제조예 1의 재료 종류 및 배합비로 고형차를 제조하는 것이 고형차의 기호성을 높일 수 있을 것으로 판단된다.As a result, as compared to Table 2 below, the solid difference of Preparation Example 1 showed the highest score, and the solid difference of Comparative Example 2 showed the lowest overall score. Therefore, it is determined that the palatability of the solid tea can be improved by preparing the solid tea with the material type and mixing ratio of Preparation Example 1.

쌍화농축액 및 독활혼합농축액의 배합비에 따른 고형차의 관능검사Sensory test of solid difference according to the mixing ratio of Ssanghwa Concentrate and Toxin-Active Mixture Concentrate 구분division color incense taste 전체적인 기호도overall sign 제조예 1Preparation Example 1 5.705.70 5.925.92 6.146.14 5.905.90 비교예 1Comparative Example 1 5.545.54 4.884.88 5.305.30 5.345.34 비교예 2Comparative Example 2 5.505.50 4.704.70 4.324.32 4.624.62 비교예 3Comparative Example 3 5.685.68 5.045.04 5.485.48 5.565.56

실시예 5. 고형차 배합비에 따른 관능검사Example 5. Sensory test according to the solid tea blending ratio

제조예 1의 고형차와 제조예 1의 방법으로 고형차를 제조하되 (5)단계의 재료 종류 및 배합비를 달리한 고형차(비교예 4 및 5)를 가지고 실시예 4와 동일한 방법으로 관능검사를 실시하였다.The solid tea of Preparation Example 1 and the solid tea of Preparation Example 1 were prepared, but with the solid difference (Comparative Examples 4 and 5) having different material types and mixing ratios in step (5), sensory examination was performed in the same manner as in Example 4 was carried out.

고형차 배합비solid tea blending ratio 고형차 재료 종류Types of solid tea materials 제조예 1Preparation Example 1 비교예 4Comparative Example 4 비교예 5Comparative Example 5 쌍화농축액 분말Ssanghwa Concentrate Powder 4.24.2 4.24.2 33 독활 혼합추출분말Toxic activity mixed extract powder 1One 1One 22 백설탕white sugar 63.863.8 67.867.8 5959 말토덱스트린maltodextrin 2222 2222 2525 호두 분쇄물walnut crushed 22 -- 33 대추 분쇄물jujube crushed 22 22 33 생강엑기스 분말Ginger Extract Powder 0.50.5 0.50.5 0.20.2 소금salt 0.50.5 0.50.5 0.80.8 아몬드almond 22 -- 33 전분starch 22 22 1One

그 결과, 하기 표 4에 비교한 바와 같이, 제조예 1의 고형차가 향, 맛 및 전체적인 기호도에서 비교예 4 및 5의 고형차에 비해 높은 점수를 나타내어, 제조예 1과 같이 10가지 재료를 모두 적정량 배합하여 고형차를 제조하는 것이 쌍화농축액 및 독활 혼합추출물과 부재료들이 조화를 이루어 기호도가 최적화됨을 확인할 수 있었다.As a result, as compared to Table 4 below, the solid difference of Preparation Example 1 showed a higher score than the solid difference of Comparative Examples 4 and 5 in flavor, taste, and overall preference, so that all 10 ingredients were prepared as in Preparation Example 1. It was confirmed that the preference was optimized as the Ssanghwa Concentrate and the Toxin Active Mixed Extract and the auxiliary materials were harmonized to prepare the solid tea by mixing the appropriate amount.

고형차 배합비에 따른 관능검사Sensory test according to the blending ratio of solid tea 구분division color incense taste 전체적인 기호도overall sign 제조예 1Preparation Example 1 5.705.70 5.925.92 6.146.14 5.905.90 비교예 4Comparative Example 4 5.725.72 5.485.48 5.845.84 5.685.68 비교예 5Comparative Example 5 5.605.60 5.145.14 5.325.32 5.345.34

Claims (5)

(1) 당귀, 천궁, 작약, 감초, 계피, 숙지황 및 황기의 쌍화재료에 주정을 첨가한 후 추출하고 농축하여 쌍화농축액을 제조하는 단계;
(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 혼합한 후 분무건조하여 쌍화농축액 분말을 제조하는 단계;
(3) 독활, 도라지, 감초, 뽕나무열매, 건강, 구기자나무뿌리 및 모창출의 독활혼합재료에 물을 첨가하여 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계;
(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 혼합한 후 살균하고 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및
(5) 고형차 총 중량 기준으로, 상기 (2)단계의 제조한 쌍화농축액 분말 4~4.5 중량% 및 상기 (4)단계의 제조한 독활 혼합추출분말 0.8~1.2 중량%와 설탕 63~65 중량%, 말토덱스트린 21~23 중량%, 호두 분쇄물 1.5~2.5 중량%, 대추 분쇄물 1.5~2.5 중량%, 생강엑기스 분말 0.4~0.6 중량%, 소금 0.4~0.6 중량%, 아몬드 1.5~2.5 중량% 및 전분 1.5~2.5 중량%를 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법.
(1) extracting and concentrating Ssanghwa concentrate by adding alcohol to the ingredients of Ssanghwa of Angelica, Chunkyung, Peony, Licorice, Cinnamon, Sukhumi Hwang and Hwanggi;
(2) mixing dextrin with the Ssanghwa concentrate prepared in step (1), and then spray-drying to prepare a powder of the Ssanghwa concentrate;
(3) preparing a poisonous mixture concentrate by adding water to the poisonous active mixture, bellflower, licorice, mulberry fruit, health, goji berry root and seedling, followed by filtration and concentration;
(4) mixing dextrin with the poisonous activity mixed concentrate prepared in step (3), followed by sterilization and hot air drying to prepare a poisonous activity mixed extract powder; and
(5) Based on the total weight of the solid tea, 4 to 4.5 wt% of the Ssanghwa Concentrate powder prepared in step (2) and 0.8 to 1.2 wt% of the poisonous active mixed extract powder prepared in the step (4) and 63 to 65 wt% of sugar %, maltodextrin 21-23 wt%, walnut pulverized 1.5-2.5 wt%, jujube pulverized 1.5-2.5 wt%, ginger extract powder 0.4-0.6 wt%, salt 0.4-0.6 wt%, almond 1.5-2.5 wt% and 1.5 to 2.5 wt% of starch.
삭제delete 제1항에 있어서,
(1) 쌍화재료 총 중량 기준으로, 당귀 25~29 중량%, 천궁 21~25 중량%, 작약 7~9 중량%, 감초 9~11 중량%, 계피 15~17 중량%, 숙지황 7~9 중량% 및 황기 7~9 중량%의 쌍화재료에 주정을 첨가한 후 추출하고 농축하여 쌍화농축액을 제조하는 단계;
(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 혼합한 후 분무건조하여 쌍화농축액 분말을 제조하는 단계;
(3) 독활혼합재료 총 중량 기준으로, 독활 20~24 중량%, 도라지 20~24 중량%, 감초 20~24 중량%, 뽕나무열매 10~12 중량%, 건강 10~12 중량%, 구기자나무뿌리 5~7 중량% 및 모창출 5~7 중량%의 독활혼합재료에 물을 첨가하여 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계;
(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 혼합하고 살균한 후 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및
(5) 고형차 총 중량 기준으로, 상기 (2)단계의 제조한 쌍화농축액 분말 4~4.5 중량% 및 상기 (4)단계의 제조한 독활 혼합추출분말 0.8~1.2 중량%와 설탕 63~65 중량%, 말토덱스트린 21~23 중량%, 호두 분쇄물 1.5~2.5 중량%, 대추 분쇄물 1.5~2.5 중량%, 생강엑기스 분말 0.4~0.6 중량%, 소금 0.4~0.6 중량%, 아몬드 1.5~2.5 중량% 및 전분 1.5~2.5 중량%를 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법.
The method of claim 1,
(1) Based on the total weight of Ssanghwa ingredients, Angelica 25~29 wt%, Cheongung 21~25 wt%, Peony 7~9 wt%, Licorice 9~11 wt%, Cinnamon 15~17 wt%, Sukhumi Hwang 7~9 wt% % and 7 to 9% by weight of Astragalus Ssanghwa after adding alcohol to the material, extracting and concentrating to prepare a Ssanghwa concentrate;
(2) mixing dextrin with the Ssanghwa concentrate prepared in step (1), and then spray-drying to prepare a powder of the Ssanghwa concentrate;
(3) Based on the total weight of the poisonous mixture, 20~24% by weight of poisonous activity, 20~24% by weight of bellflower, 20~24% by weight of licorice, 10~12% by weight of mulberry fruit, 10~12% by weight of health, Goji berry root 5 to 7% by weight and 5 to 7% by weight of hair-creating poisonous activity mixture by adding water to extract, followed by filtration and concentration to prepare a poisonous activity mixture concentrate;
(4) mixing dextrin with the poisonous activity mixed concentrate prepared in step (3), sterilizing, and drying with hot air to prepare a poisonous activity mixed extract powder; and
(5) Based on the total weight of the solid tea, 4 to 4.5 wt% of the Ssanghwa Concentrate powder prepared in step (2) and 0.8 to 1.2 wt% of the poisonous active mixed extract powder prepared in the step (4) and 63 to 65 wt% of sugar %, maltodextrin 21-23 wt%, walnut pulverized 1.5-2.5 wt%, jujube pulverized 1.5-2.5 wt%, ginger extract powder 0.4-0.6 wt%, salt 0.4-0.6 wt%, almond 1.5-2.5 wt% and 1.5 to 2.5 wt% of starch.
제3항에 있어서,
(1) 쌍화재료 총 중량 기준으로, 당귀 25~29 중량%, 천궁 21~25 중량%, 작약 7~9 중량%, 감초 9~11 중량%, 계피 15~17 중량%, 숙지황 7~9 중량% 및 황기 7~9 중량%의 쌍화재료에 45~55% 주정 8~10배(v/w) 첨가한 후 90~95℃에서 10~14시간 동안 추출하고 농축하여 쌍화농축액을 제조하는 단계;
(2) 상기 (1)단계의 제조한 쌍화농축액에 덱스트린을 2.5~3.5:6.5~7.5(v:w) 비율로 혼합한 후 180~190℃에서 분무건조하여 쌍화농축액 분말을 제조하는 단계;
(3) 독활혼합재료 총 중량 기준으로, 독활 20~24 중량%, 도라지 20~24 중량%, 감초 20~24 중량%, 뽕나무열매 10~12 중량%, 건강 10~12 중량%, 구기자나무뿌리 5~7 중량% 및 모창출 5~7 중량%의 독활혼합재료에 물을 7~9배(v/w) 첨가한 후 90~110℃에서 24~30시간 동안 추출한 후 여과 및 농축하여 독활혼합농축액을 제조하는 단계;
(4) 상기 (3)단계의 제조한 독활혼합농축액에 덱스트린을 2.5~3.5:6.5~7.5(v:w) 비율로 혼합한 후 80~85℃에서 15~20분 동안 살균한 후 열풍건조하여 독활 혼합추출분말을 제조하는 단계; 및
(5) 고형차 총 중량 기준으로, 상기 (2)단계의 제조한 쌍화농축액 분말 4~4.5 중량% 및 상기 (4)단계의 제조한 독활 혼합추출분말 0.8~1.2 중량%와 설탕 63~65 중량%, 말토덱스트린 21~23 중량%, 호두 분쇄물 1.5~2.5 중량%, 대추 분쇄물 1.5~2.5 중량%, 생강엑기스 분말 0.4~0.6 중량%, 소금 0.4~0.6 중량%, 아몬드 1.5~2.5 중량% 및 전분 1.5~2.5 중량%를 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고형차의 제조방법.
4. The method of claim 3,
(1) Based on the total weight of Ssanghwa ingredients, Angelica 25~29 wt%, Cheongung 21~25 wt%, Peony 7~9 wt%, Licorice 9~11 wt%, Cinnamon 15~17 wt%, Sukhumi Hwang 7~9 wt% % and 7 to 9% by weight of Ssanghwa material, 45 to 55% alcohol 8 to 10 times (v/w), followed by extraction and concentration at 90 to 95° C. for 10 to 14 hours to prepare a Ssanghwa concentrate;
(2) mixing dextrin with the Ssanghwa concentrate prepared in step (1) in a ratio of 2.5 to 3.5:6.5 to 7.5 (v:w) and spray-drying at 180 to 190° C. to prepare a powder of the Ssanghwa concentrate;
(3) Based on the total weight of the poisonous mixture, 20~24% by weight of poisonous activity, 20~24% by weight of bellflower, 20~24% by weight of licorice, 10~12% by weight of mulberry fruit, 10~12% by weight of health, Goji berry root After adding 7-9 times (v/w) of water to 5-7 wt % and 5-7 wt % of hair-creating poisonous mixture, extraction is performed at 90-110° C. for 24-30 hours, followed by filtration and concentration for poison-active mixing preparing a concentrate;
(4) After mixing dextrin in the ratio of 2.5 to 3.5:6.5 to 7.5 (v:w) in the poisonous activity mixed concentrate prepared in step (3), sterilize at 80 to 85° C. for 15 to 20 minutes, and then dry with hot air. Preparing a poison active mixed extract powder; and
(5) Based on the total weight of the solid tea, 4 to 4.5 wt% of the Ssanghwa Concentrate powder prepared in step (2) and 0.8 to 1.2 wt% of the poisonous active mixed extract powder prepared in the step (4) and 63 to 65 wt% of sugar %, maltodextrin 21-23 wt%, walnut pulverized 1.5-2.5 wt%, jujube pulverized 1.5-2.5 wt%, ginger extract powder 0.4-0.6 wt%, salt 0.4-0.6 wt%, almond 1.5-2.5 wt% and 1.5 to 2.5 wt% of starch.
제1항, 제3항, 제4항 중 어느 한 항의 방법으로 제조된 고형차.A solid tea manufactured by the method of any one of claims 1, 3, and 4.
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티스토리 김종필 기자의 헬스케어 뉴스에 게재된 ‘함소아제약, 독립활력 쌍화 판매’(2020.07.23.)(공지예외미주장문헌)*

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