KR102163281B1 - Method for suppressing over-growth of tomato plug seedling by control of day and night temperature - Google Patents

Method for suppressing over-growth of tomato plug seedling by control of day and night temperature Download PDF

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KR102163281B1
KR102163281B1 KR1020190000503A KR20190000503A KR102163281B1 KR 102163281 B1 KR102163281 B1 KR 102163281B1 KR 1020190000503 A KR1020190000503 A KR 1020190000503A KR 20190000503 A KR20190000503 A KR 20190000503A KR 102163281 B1 KR102163281 B1 KR 102163281B1
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seedlings
plug
tomato
temperature
treatment
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KR20190083310A (en
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황승재
김혜민
이혜리
정현우
김현민
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경상대학교산학협력단
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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Abstract

본 발명은 주야간 온도 조절에 의한 토마토 플러그묘의 도장 억제 방법에 관한 것으로, 본 발명의 도장 억제 방법은 친환경적인 방법으로 밀식재배되는 플러그묘의 도장을 억제하고 묘소질을 향상시킬 수 있어, 양질의 토마토 플러그묘를 대량으로 생산할 수 있을 것이다.The present invention relates to a method for inhibiting painting of tomato plug seedlings by controlling day and night temperature, and the paint inhibiting method of the present invention can suppress the painting of plug seedlings that are closely cultivated in an eco-friendly manner and improve the quality of seedlings. Can be produced in large quantities.

Description

주야간 온도 조절에 의한 토마토 플러그묘의 도장 억제 방법{Method for suppressing over-growth of tomato plug seedling by control of day and night temperature}Method for suppressing over-growth of tomato plug seedling by control of day and night temperature}

본 발명은 주야간 온도 조절에 의한 토마토 플러그묘의 도장 억제 방법에 관한 것이다.The present invention relates to a method for inhibiting painting of tomato plug seedlings by controlling day and night temperature.

작물 모종(苗種)의 소질이 정식(定植) 후 본포(本圃)에서의 생육이나 수량, 품질에 큰 영향을 미치기 때문에, 작물재배에 있어서 육묘(育苗)는 매우 중요하며, 양질의 묘를 생산하기 위해서는 많은 노력과 기술이 필요 된다. 최근에는 육묘와 재배가 분업화되어 전문적으로 모종을 생산, 판매하는 플러그 육묘가 증가하는 추세이다.Since the quality of crop seedlings greatly affects the growth, yield, and quality of the plant after planting, seedlings are very important in crop cultivation, and high-quality seedlings are produced. To do this, a lot of effort and skill are required. In recent years, seedlings and cultivation have been divided into labor, and plug seedlings that professionally produce and sell seedlings are increasing.

플러그묘(plug seedling)란 여러 개의 작은 용기(cell)가 연결된 플러그 트레이라고 불리는 육묘 전용 용기에서 생산된 묘를 말한다. 플러그묘는 양질의 균일한 묘를 대량으로 생산하는 것을 목적으로 하며, 육묘 전용 시설, 장비 및 자재를 갖추고, 상토 제조 및 충전, 파종, 관수, 시비 및 환경조건 등 파종 준비부터 육묘 종료까지의 작업이 유기적으로 연결되어 이루어지기 때문에 넓은 의미에서 공정(육)묘라고 하기도 한다. 우리나라는 농업기계화연구소에서 1990년에 플러그묘 생산기술을 시험적으로 도입하였고, 1992년 흥농종묘에서 플러그묘를 생산, 판매하기 시작하였다. 이후, 시설면적 확대 및 연중 묘 생산 증가, 농업 노동력의 감소 등 채소 생산여건의 변화에 따라 채소 플러그묘를 전문적으로 생산하는 공정육묘장의 수가 지속적으로 증가하였다.Plug seedling refers to a seedling produced in a container dedicated to seedlings called a plug tray in which several small cells are connected. Plug seedlings are aimed at mass production of high-quality and uniform seedlings, and are equipped with facilities, equipment, and materials for seedlings, and work from preparation to seedling to completion of seedlings such as soil manufacturing and filling, seeding, irrigation, fertilization and environmental conditions. Because it is organically connected, it is sometimes referred to as a process (six) seedling in a broad sense. In Korea, the Agricultural Mechanization Research Institute introduced the plug seedling production technology in 1990, and in 1992 Heungnong Seedling began producing and selling plug seedlings. Since then, the number of process nurseries that professionally produce vegetable plug seedlings has continuously increased due to changes in vegetable production conditions, such as expanding the facility area, increasing seedling production throughout the year, and decreasing agricultural labor.

그러나, 플러그 육묘는 기계화와 육묘 효율을 높이는 것이 중요한 목표였기 때문에 고밀도 육묘와 기계화 작업으로 인한 묘의 품질저하가 문제가 되고 있다. 고밀도 육묘시 발생하는 대표적인 문제점으로는 묘의 웃자람 현상(도장, 徒長)으로서, 특히, 토마토 등의 가지과 작물의 경우 절간이 지나치게 도장하는 문제점이 보고되고 있다.However, since it was an important goal to increase the mechanization and seedling efficiency of the plug seedling, the quality of the seedlings due to the high-density seedling and mechanization work is a problem. A typical problem that occurs during high-density seedling is the overgrowth of seedlings (painting, 徒長). In particular, in the case of eggplant crops such as tomatoes, the problem of excessive coating between the nodes has been reported.

나고야 의정서(Nagoya Protocol) 발효 및 해외 생약식물 원재료 가격 상승에 대비하기 위해서는, 국내 신의약 산업에 이용되는 고기능성 식물 원료의 안정적이고 경제적인 수급을 위한 대량생산 시스템의 개발 및 구축이 시급하다.In order to prepare for the entry into force of the Nagoya Protocol and rising prices of raw materials for herbal medicines abroad, it is urgent to develop and establish a mass production system for the stable and economical supply and demand of high-functional plant raw materials used in the domestic new medicine industry.

한편, 한국등록특허 제0477262호에는 피토크롬 PHYA 유전자를 이용한 '경작식물의 밀식재배시 도장현상을 억제하는 방법'이 개시되어 있고, 한국등록특허 제0774862호에는 '국화 꽃목 도장 억제제'가 개시되어 있으나, 본 발명의 주야간 온도 조절에 의한 토마토 플러그묘의 도장 억제 방법에 대해서는 기재된 바가 없다.On the other hand, Korean Patent No. 0476262 discloses a'method for suppressing the painting phenomenon during dense cultivation of cultivated plants' using the phytochrome PHYA gene, and Korean Patent No. 0774862 discloses a'chrysanthemum flower tree painting inhibitor'. , There is no description of the method of inhibiting painting of tomato plug seedlings by day and night temperature control of the present invention.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명자들은 토마토 육묘 시 고온 및 밀식 재배로 인해 발생하는 도장(웃자람) 현상을 억제하기 위해서, 토마토 플러그묘 육묘 시, 주간(명기)에는 18℃의 온도, 야간(암기)에는 25℃의 온도로 각각 12시간씩 처리한 경우에 토마토 플러그묘의 도장 현상이 억제되는 것을 확인함으로써, 본 발명을 완성하였다.The present invention was derived from the above requirements, the inventors of the present invention in order to suppress the paint (upper) phenomenon caused by high temperature and wheat cultivation during tomato seedling, when seedling tomato plug seedlings, 18 ℃ during the day (specified) The present invention was completed by confirming that the painting phenomenon of tomato plug seedlings was suppressed when each treatment was performed at a temperature of 25° C. for 12 hours at a temperature of and at night (memorization).

상기 과제를 해결하기 위해, 본 발명은 발아된 토마토가 식재된 플러그 트레이를 주야간 온도차(difference between day and night temperature)가 조절된 시스템에서 육묘시키는 단계를 포함하는 토마토 플러그묘의 도장을 억제하는 방법을 제공한다.In order to solve the above problem, the present invention provides a method for inhibiting the painting of tomato plug seedlings comprising the step of seeding a plug tray in which germinated tomatoes are planted in a system in which the difference between day and night temperature is controlled do.

또한, 본 발명은 발아시킨 토마토가 식재된 플러그 트레이를 16~20℃의 명기 온도 및 23~27℃의 암기 온도로 조절한 시스템 내에서 육묘시키는 단계를 포함하는 도장이 억제된 토마토 플러그묘의 재배 방법을 제공한다.In addition, the present invention is a method of cultivation of tomato plug seedlings with suppressed painting comprising the step of seeding a plug tray in which germinated tomatoes are planted in a system controlled at a specified temperature of 16 to 20°C and a memorization temperature of 23 to 27°C Provides.

또한, 본 발명은 상기 방법에 의해 재배된 도장이 억제된 토마토 플러그묘를 제공한다.In addition, the present invention provides a tomato plug seedlings with suppressed painting cultivated by the method.

본 발명은 방법은 토마토 플러그묘 육묘시 발생하는 도장 현상을 효과적으로 억제할 수 있는 친환경적인 방법으로, 주야간 온도 조절을 통해 밀식재배되는 플러그묘의 도장을 억제하고 묘소질을 향상시킬 수 있어, 양질의 토마토 플러그묘를 대량으로 생산할 수 있을 것이다.The method of the present invention is an eco-friendly method that can effectively suppress the painting phenomenon that occurs during seedling of tomato plug seedlings, and it is possible to suppress the painting of plug seedlings that are cultivated densely through temperature control during the day and night and improve seedling quality. You will be able to produce the graves in large quantities.

도 1은 주야간 온도차(DIF)를 이용한 플러그묘의 밀식재배에 따른 도장 억제 및 묘소질 향상 실험의 배치도이다.
도 2는 주야간 온도차(DIF) 처리 조건별 토마토 '미니찰' 플러그묘의 생육을 보여주는 사진이다.
도 3은 주야간 온도차(DIF) 처리에서 양액의 전기전도도 수준에 따른 토마토 '미니찰' 플러그묘의 생육을 보여주는 사진이다.
1 is a layout diagram of an experiment for suppressing painting and improving seedling quality according to dense cultivation of plug seedlings using a temperature difference between day and night (DIF).
FIG. 2 is a photograph showing the growth of tomato'minichal' plug seedlings according to day and night temperature difference (DIF) treatment conditions.
3 is a photograph showing the growth of tomato'minichal' plug seedlings according to the electrical conductivity level of a nutrient solution in day and night temperature difference (DIF) treatment.

본 발명의 목적을 달성하기 위하여, 본 발명은 발아된 토마토가 식재된 플러그 트레이를 주야간 온도차(difference between day and night temperature)가 조절된 시스템에서 육묘시키는 단계를 포함하는 토마토 플러그묘의 도장을 억제하는 방법을 제공한다.In order to achieve the object of the present invention, the present invention is a method for inhibiting painting of tomato plug seedlings comprising the step of seeding a plug tray in which germinated tomatoes are planted in a system in which the difference between day and night temperature is controlled Provides.

용어 '주야간 온도차'는 주간 온도(낮기온)에서 야간 온도(밤기온)를 뺀 것을 의미하는 것으로, 본 발명의 일 구현예에 따른 방법에 있어서, 상기 주야간 온도차는 -5~9℃일 수 있고, 바람직하게는 -7℃의 온도차일 수 있으나, 이에 제한되지 않는다.The term'day and night temperature difference' means that the daytime temperature (lower temperature) is subtracted from the nighttime temperature (night temperature), and in the method according to an embodiment of the present invention, the day and night temperature difference may be -5 to 9°C, and , Preferably it may be a temperature difference of -7 ℃, but is not limited thereto.

본 발명에 따른 주야간 온도는 구체적으로는, 16~20℃의 명기(주간) 온도 및 23~27℃의 암기(야간) 온도로 조절되는 것일 수 있고, 바람직하게는 18℃의 명기 온도 및 25℃의 암기 온도로 조절되는 것일 수 있으나, 이에 제한되지 않으며, 상기 명기 및 암기 온도의 처리시간은 각각 12시간으로 동일하다.The day and night temperature according to the present invention may be controlled to a specific (daytime) temperature of 16 to 20°C and a memorization (night) temperature of 23 to 27°C, preferably 18°C and 25°C. It may be adjusted to the memorization temperature of, but is not limited thereto, and the treatment time of the memorization temperature and the memorization temperature is the same as 12 hours, respectively.

본 발명의 토마토 플러그묘의 도장을 억제하는 방법에 있어서, 상기 토마토는 '미니찰(Solanum lycopersicum L. 'MiniChal')' 품종일 수 있으나, 이에 제한되지 않는다.In the method for inhibiting the painting of tomato plug seedlings of the present invention, the tomato may be a'minichal' ( Solanum lycopersicum L.'MiniChal') variety, but is not limited thereto.

용어 '플러그묘'는 응집성이 있는 소량의 배지가 담긴 개개의 셀에서 생육된 묘를 의미하는 것으로, 플러그묘 생산시스템에서는 종자를 기계적으로 수십 내지 수백 개의 셀을 가진 플러그 트레이에 파종하여 한 셀에서 하나의 식물체가 생산된다. 재배방식과 비교하여 파종 속도와 정확성이 높고, 묘가 균일하며, 이식작업 시 상처를 줄이고, 묘의 생장속도가 빠르고, 묘의 수송과 취급이 용이하며, 공간 이용 효율이 좋고, 노동력이 적게 드는 장점이 있다.The term'plug seedling' refers to a seedling grown in individual cells containing a small amount of cohesive medium. In a plug seedling production system, seeds are mechanically sown in plug trays having tens to hundreds of cells and One plant is produced. Compared to the cultivation method, the sowing speed and accuracy are high, the seedling is uniform, the wounds are reduced during transplantation, the growth rate of the seedlings is fast, the transport and handling of the seedlings are easy, the space utilization efficiency is good, and the labor force is reduced. have.

그러나, 플러그묘는 밀식 조건이라 도장(웃자람)하기 쉬운 단점이 있다. 특히, 여름 장마철과 같이 흐리고 온도와 습도가 높은 환경에서는 플러그묘는 급격한 줄기신장으로 연약하게 자라기 때문에, 많은 육묘장에서 살균제로 등록된 트리아졸계 화합물을 사용하여 도장을 억제하고 있다. 그러나, 트리아졸계 화합물은 식물체 내 지속성이 길어서 활착 및 초기생육 지연을 야기할 수 있고, 높은 농도로 처리하였을 경우 회복되지 못하는 보다 큰 문제를 발생시킬 수 있다. 또한, 환경오염과 농산물 안전성에 대한 인식이 높아지면서 일부 국가에서는 화학적 생장조절제의 상용을 강력하게 규제하고 있으며, 소비자들도 약제를 사용한 농산물을 기피하는 추세이다.However, the plug seedling has a disadvantage that it is easy to paint (laugh) because it is in a tight condition. In particular, in a cloudy, high temperature and humidity environment such as the summer rainy season, plug seedlings grow weakly due to rapid stem growth, so many nurseries use a triazole-based compound registered as a fungicide to suppress painting. However, since the triazole-based compound has long persistence in the plant, it may cause a delay in survival and initial growth, and may cause a greater problem of not recovering when treated with a high concentration. In addition, as awareness of environmental pollution and agricultural product safety has increased, some countries are strongly restricting the use of chemical growth regulators, and consumers are also inclined to avoid agricultural products using drugs.

본 발명은 또한, 발아시킨 토마토가 식재된 플러그 트레이를 16~20℃의 명기 온도 및 23~27℃의 암기 온도로 조절한 시스템 내에서 육묘시키는 단계를 포함하는 도장이 억제된 토마토 플러그묘의 재배 방법을 제공한다. 상기 재배 방법은 구체적으로는,The present invention is also a method of cultivation of tomato plug seedlings with suppressed painting comprising the step of seeding the plug tray in which the germinated tomatoes are planted in a system controlled at a specified temperature of 16 to 20°C and a memorization temperature of 23 to 27°C Provides. Specifically, the cultivation method,

(1) 상토가 충진된 플러그 트레이에 토마토의 종자를 파종하는 단계;(1) sowing seeds of tomatoes in a plug tray filled with top soil;

(2) 상기 토마토 종자가 파종된 플러그 트레이를 120 내지 180μmol·m-2·s-1 범위의 광도, 18 내지 22℃의 온도를 유지하는 시스템 내에서, 0.8 내지 1.2dS·m-1의 전기전도도를 갖는 양액을 플러그 트레이에 저면관수로 적용하며 6~18일 동안 발아시키는 단계; 및(2) In a system that maintains a light intensity in the range of 120 to 180 μmol·m -2 ·s -1 and a temperature in the range of 18 to 22°C, electricity of 0.8 to 1.2 dS·m -1 Applying the nutrient solution having conductivity to the plug tray by bottom irrigation and germinating for 6 to 18 days; And

(3) 상기 발아시킨 토마토가 식재된 플러그 트레이를 16~20℃의 명기 온도 및 23~27℃의 암기 온도로 조절한 시스템 내에서 22~39일 동안 육묘시키는 단계;를 포함하는 것일 수 있으나, 이에 제한되지 않는다.(3) seeding the plug tray in which the germinated tomatoes are planted for 22 to 39 days in a system controlled at a specified temperature of 16 to 20°C and a memorization temperature of 23 to 27°C, but may include, It is not limited thereto.

본 발명에 따른 재배 방법에 의해 재배된 토마토 플러그묘는 초장(plant height), 하배축 길이(hypocotyl length), 절간장(internode length), 경경(stem diameter), 엽장(leaf length), 엽폭(leaf width) 또는 엽면적(leaf area)이 감소된 것이 특징일 수 있으나, 이에 제한되지 않는다.Tomato plug seedlings grown by the cultivation method according to the present invention are plant height, hypocotyl length, internode length, stem diameter, leaf length, and leaf width. Alternatively, a leaf area may be reduced, but is not limited thereto.

본 발명은 또한, 상기 방법에 의해 재배된 도장이 억제된 토마토 플러그묘를 제공한다.
The present invention also provides a tomato plug seedling with suppressed paint cultivated by the method.

이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

1. 실험 방법1. Experimental method

2017년 2월 16일 토마토 묘(Solanum lycopersicum L. 'MiniChal')를 상업적 공정육묘장에서 구입하여 2017년 2월 28일부터 경상대학교 시설원예학연구실에 위치한 밀폐형 식물생산 시스템(C1200H3, FC Poibe Co. Ltd., 한국)에서 주야간 온도차 처리를 하였다. 밀폐형 식물생산 시스템 내의 육묘환경은 광도 150±10μmol·m-2·s-1 PPFD, 광주기 12/12시간(명기/암기), 광원 형광등(FHF32SSEX-D, Osram Co. Ltd., 독일)으로 설정하였다. 양액은 pH 6.5 및 전기전도도(EC) 1.5dS·m-1 로 처리 직후부터 2-3일 간격으로 저면관수하였다.On February 16, 2017, tomato seedlings ( Solanum lycopersicum L.'MiniChal') were purchased at a commercial process nursery, and from February 28, 2017, a closed plant production system (C1200H3, FC Poibe Co. Ltd.) located in the Horticultural Laboratory of Gyeongsang National University. ., Korea) in the day and night temperature difference treatment. The seedling environment in the enclosed plant production system is luminous intensity 150±10μmol·m -2 ·s -1 PPFD, photoperiod 12/12 hours (specify/memorize), light source fluorescent lamp (FHF32SSEX-D, Osram Co. Ltd., Germany). Set. The nutrient solution was irrigated to the bottom at intervals of 2-3 days from immediately after treatment with pH 6.5 and electrical conductivity (EC) of 1.5 dS m -1 .

본 발명에 사용된 양액 조성Nutrient solution composition used in the present invention ChemicalChemical 농도(㎎·L-1)Concentration (mg·L -1 ) ChemicalChemical 농도(㎎·L-1)Concentration (mg·L -1 ) Ca(NO3)2·4H2OCa(NO 3 ) 2 4H 2 O 1,227.21,227.2 H3BO3 H 3 BO 3 1.841.84 KNO3 KNO 3 444.4444.4 MnSO4·H2OMnSO 4 H 2 O 2.162.16 KH2PO4 KH 2 PO 4 204.0204.0 CuSO4·5H2OCuSO 4 5H 2 O 0.200.20 NH4NO3 NH 4 NO 3 96.096.0 ZnSO4·7H2OZnSO 4 7H 2 O 0.600.60 MgSO4·7H2OMgSO 4 7H 2 O 565.8565.8 Na2MoO4·2H2ONa 2 MoO 4 2H 2 O 0.400.40 Fe-EDTAFe-EDTA 5.675.67 K2SO4 K 2 SO 4 356.7356.7

2. 주야간 온도차(difference between day and night temperature, DIF) 처리 조건2. Difference between day and night temperature (DIF) treatment conditions

주야간 온도차(이하 DIF) 처리 조건은 다음과 같다. 먼저, 대조구로는 +7 DIF[25/18℃(명기 12시간/암기 12시간)] 및 디니코나졸(Diniconazole)을 2회 처리(150mg·L-1)하였고, 시험구로는 +7 DIF[25℃/18℃(명기 12시간/암기 12시간)과, -7 DIF[18℃/25℃(명기 12시간/암기 12시간)], 그리고 Temp. drop[26℃/14.5℃/19℃(명기 10.5시간/일출시 3시간/암기 10.5시간)]의 세가지 조건을 각각 처리하였다. 대조구를 포함한 모두 처리구의 일평균 기온을 21.5℃로 동일하게 처리하였다. 이는 적산온도를 동일하게 하여 적산온도 차이가 주야간 온도차 처리에 영향을 미치지 않도록 하기 위함이다. 주야간 온도차는 2017년 2월 28일부터 처리하여 2017년 3월 24일에 최종 생육을 조사하였다.
Day and night temperature difference (hereinafter, DIF) processing conditions are as follows. First, as a control group, +7 DIF[25/18°C (12 hours/memory 12 hours)] and Diniconazole were treated twice (150mg·L -1 ), and +7 DIF[ 25℃/18℃(specified 12 hours/memorized 12 hours), -7 DIF[18℃/25℃(specified 12 hours/memorized 12 hours)], and Temp. Three conditions of drop[26℃/14.5℃/19℃ (specified 10.5 hours/sunrise 3 hours/memorized 10.5 hours)] were each treated. The average daily temperature of all treatment groups including the control group was treated equally at 21.5°C. This is to make the integration temperature the same so that the difference in integration temperature does not affect the treatment of temperature difference between day and night. Day and night temperature differences were treated from February 28, 2017, and final growth was investigated on March 24, 2017.

3. 주야간 온도차(DIF) 및 양액의 전기전도도 수준별 처리3. Day and night temperature difference (DIF) and treatment of nutrient solution by level of electrical conductivity

토마토 플러그묘의 도장을 억제하기 위한 주야간 온도차 처리 조건에서 양액의 적정 전기전도도(electrical conductivity, EC) 수준을 구명하기 위해, -7 DIF 처리 시 양액의 EC를 0.5, 1.0 또는 1.5dS·m-1 로 설정하여 관수하며 토마토 플러그묘의 생장 특성을 분석하였다. 실험은 2017년 11월 27일에 파종하고, 주야간 온도차 및 양액 처리는 2017년 12월 4일에 시작하였으며, 최종 생육은 2018년 1월 10일에 분석하였다.To determine the optimal electrical conductivity (EC) level of the nutrient solution under the conditions of day and night temperature difference treatment to suppress the painting of tomato plug seedlings, the EC of the nutrient solution was changed to 0.5, 1.0 or 1.5 dS m -1 during -7 DIF treatment. It was set and watered, and the growth characteristics of tomato plug seedlings were analyzed. The experiment was sown on November 27, 2017, the temperature difference between day and night and nutrient solution treatment started on December 4, 2017, and the final growth was analyzed on January 10, 2018.

관수는 매일 오전 08:30에 저면관수로 처리하였으며, 양액의 pH는 6.5로 동일하게 하였고, 그 외 광원 및 광도 조건은 전술한 방법과 동일하게 수행하였다. 대조구로는 +7 DIF[25/18℃(명기 12시간/암기 12시간)] + EC 0.5dS·m-1의 양액; 및 +7 DIF + 디니코나졸(150mg·L-1) 2회 + EC 0.5dS·m-1의 양액; 처리를 사용하였고, 시험구로는 -7 DIF[18℃/25℃(명기 12시간/암기 12시간)] + EC 0.5dS·m-1의 양액; -7 DIF + EC 1.0dS·m-1의 양액; -7 DIF + EC 1.5dS·m-1의 양액; 처리를 사용하였다. 대조구를 포함한 모두 처리구의 일평균 기온을 21.5℃로 동일하게 처리하였다.
The irrigation was treated with bottom irrigation every day at 08:30 am, the pH of the nutrient solution was the same as 6.5, and other light source and luminous intensity conditions were performed in the same manner as described above. As a control, a nutrient solution of +7 DIF[25/18°C (specified 12 hours/memorized 12 hours)] + EC 0.5dS·m -1 ; And +7 DIF + diniconasol (150mg·L -1 ) twice + EC 0.5dS·m -1 nutrient solution; Treatment was used, and as a test group, -7 DIF[18° C./25° C. (specified 12 hours/memorized 12 hours)] + EC 0.5 dS·m −1 nutrient solution; Nutrient solution of -7 DIF + EC 1.0dS·m -1 ; Nutrient solution of -7 DIF + EC 1.5 dS m -1 ; Treatment was used. The average daily temperature of all treatment groups including the control group was treated equally at 21.5°C.

4. 토마토 플러그묘의 생육 조사4. Growth investigation of tomato plug seedlings

주야간 온도차(DIF) 조건 및 주야간 온도차+양액의 전기전도도 조건에 따른 토마토 플러그묘의 생육을 조사하기 위해서, 초장, 하배축, 절간장, 경경, 엽장, 엽폭, 지상부와 지하부의 생체중 및 건물중, 엽면적, 충실도, 왜화율을 조사하였다. 지상부와 지하부의 생체중과 건물중은 전자저울(EW220-3NM, Kern&Sohn GmbH., 독일)을 이용하여 측정하였고, 엽면적은 엽면적 측정기(LI-3000, LI-COR Inc., 미국)를 사용하여 측정하였다. 건물중은 시료를 70℃ 항온 건조기(Venticell-222, MMM Medcenter Einrichtungen GmbH., 독일)에서 72시간 건조한 후 전자저울을 이용하여 측정하였다.
To investigate the growth of tomato plug seedlings according to day and night temperature difference (DIF) conditions and day and night temperature difference + electrical conductivity conditions of nutrient solution, plant height, hypocotyl, internode, length, leaf length, leaf width, live weight and dry weight in the above and underground parts, leaf area, and fidelity , The distortion rate was investigated. The live weight and dry weight of the above and underground parts were measured using an electronic scale (EW220-3NM, Kern&Sohn GmbH., Germany), and the leaf area was measured using a leaf area measuring device (LI-3000, LI-COR Inc., USA). . For dry matter, the sample was dried for 72 hours in a 70°C constant temperature dryer (Venticell-222, MMM Medcenter Einrichtungen GmbH., Germany) and then measured using an electronic balance.

실시예 1. 주야간 온도차(DIF) 처리에 따른 토마토 플러그묘의 생장 특성 분석Example 1. Analysis of growth characteristics of tomato plug seedlings according to day and night temperature difference (DIF) treatment

토마토 '미니찰' 묘의 초장, 하배축 길이, 절간장, 경경, 엽장, 엽폭, 엽면적은 디니코나졸 처리에서 생육이 가장 억제되었으며, 다음으로는 -7 DIF에서 억제되어 DIF 처리가 디니코나졸과 같이 토마토 묘의 생육을 억제시킬 수 있다는 것을 확인하였다. 지상부와 지하부의 생체중과 건물중은 +7 DIF 처리에서 가장 우수한 경향을 보였으며, 디니코나졸 처리에서 가장 저조하였다. 이는 디니코나졸 처리에 의해 묘의 생육이 억제되었기 때문이라고 판단되었다.In tomato'minichal' seedlings, plant height, lower hypocotyl length, internode length, root diameter, leaf length, leaf width, and leaf area were most inhibited by diniconasol treatment, followed by -7 DIF. It was confirmed that the growth of seedlings can be suppressed. The fresh weight and dry weight of the above-ground and underground areas showed the best tendency in +7 DIF treatment, and the lowest in diniconasol treatment. It was judged that this was because the growth of the seedlings was suppressed by the diniconasol treatment.

왜화율은 +7 DIF를 기준으로 디니코나졸 처리에서 56.5%, -7 DIF 처리에서 53.9%로 높게 나타났다. 묘의 초장이 짧고 경경이 굵은 강건한 묘를 나타내는 지표인 충실도는 디니코나졸 처리에서 가장 우수하였다. 디니코나졸 처리 다음으로 생육이 억제되었던 -7DIF 처리에서는 충실도가 저조하여 이를 증진시킬 수 있는 추가적인 연구가 필요한 것으로 사료되었다. 왜화율은 +7 DIF를 기준으로 디니코나졸 처리에서 높았으며, 다음으로는 -7 DIF 처리에서 높게 나타났다.The dwarfing rate was 56.5% in diniconasol treatment and 53.9% in -7 DIF treatment based on +7 DIF. The fidelity, which is an index indicating a robust seedling with a short plant length and a large diameter, was the best in diniconasol treatment. In the -7DIF treatment, which was suppressed after the diniconasol treatment, the fidelity was poor, and it was thought that further studies to improve this were needed. The dwarfing rate was high in diniconasol treatment based on +7 DIF, followed by -7 DIF treatment.

결과적으로 주야간 온도차를 통한 -7 DIF 처리를 통해 토마토 묘의 도장이 억제되는 효과를 확인하였으며, 추가적인 연구를 통해 묘의 도장이 억제되고 충실도가 우수한 묘를 생산할 수 있을 것이라 판단되었다.As a result, the effect of inhibiting the painting of tomato seedlings was confirmed through -7 DIF treatment through the temperature difference between day and night, and it was determined that the painting of the seedlings could be suppressed and the seedlings with excellent fidelity could be produced through additional research.

Figure 112019000513586-pat00001
Figure 112019000513586-pat00001

실시예 2. 주야간 온도차(DIF) 처리에서 양액의 전기전도도 수준에 따른 토마토 플러그묘의 생장 특성 분석Example 2. Analysis of growth characteristics of tomato plug seedlings according to the electrical conductivity level of nutrient solution in day and night temperature difference (DIF) treatment

주야간 온도차 처리와 함께 양액의 전기전도도 수준을 달리 공급하여 토마토 '미니찰'의 플러그묘를 육묘한 결과, 묘의 초장, 하배축 길이, 경경은 +7 DIF 조건의 EC 수준 0.5dS·m-1 + 디니코나졸 처리에서 가장 생육이 억제되었으며, 엽장, 엽폭, 엽면적은 -7 DIF 조건의 EC 수준 0.5dS·m-1 처리에서 억제된 경향을 보였다. 또한 -7 DIF 조건의 EC 수준 1.5dS·m-1 처리에서 토마토 '미니찰' 묘의 전반적인 생육이 증진된 경향을 보였다. 하지만 하배축은 묘의 생육이 증진되었던 -7 DIF 조건의 EC 수준 1.5dS·m-1 처리에서 보다 +7 DIF 조건의 EC 수준 0.5dS·m-1 처리에서 가장 길게 나타나 -7 DIF 조건이 하배축의 길이를 감소시킨다는 것을 확인하였다. 묘의 생체중과 건물중은 +7 DIF 조건 처리구와 -7DIF 조건의 EC 수준 0.5dS·m-1 처리에서 저조한 경향을 보였는데, 이는 상기 처리구들에서 생육이 억제되었기 때문인 것으로 판단되었다. 또한 묘의 생체중과 건물중은 -7 DIF 조건의 EC 수준 1.5dS·m-1 처리에서 가장 무거운 경향을 보였다. 지상부 건물중에 초장을 나누어 묘가 얼마나 짧고 강건한지를 나타낸 묘 소질의 지표인 충실도(compactness)는 -7 DIF 조건의 EC 수준 1.0과 1.5dS·m-1 처리에서 유의적으로 높게 나타났으며 +7 DIF 조건의 EC 수준 0.5dS·m-1 처리구에서 가장 낮았다. +7 DIF 조건의 EC 수준 0.5dS·m-1 처리를 기준으로 나타낸 왜화율은 -7 DIF 조건의 EC 수준 0.5dS·m-1 처리에서 가장 높았다. 따라서, -7 DIF 조건의 EC 수준 0.5dS·m-1 처리에서 생장조절제인 디니코나졸을 처리한 +7 DIF 조건의 EC 수준 0.5dS·m-1 처리와 유사하게 생육이 억제된 결과를 보였으나, 묘의 소질을 나타내는 충실도는 -7 DIF 조건의 EC 수준 1.0과 1.5dS·m-1에서 우수하였으며, 이 처리구에서 묘가 어느정도 왜화되었다. 결과적으로 토마토 '미니찰' 육묘 시 도장을 억제시키면서 묘소질을 높이기 위해서 -7 DIF 조건에서 양액의 EC 수준을 1.0dS·m-1 범위로 공급하는 것이 적절할 것으로 판단되었다.As a result of seedling tomato'minichal' plug seedlings by supplying different electrical conductivity levels of nutrient solution along with temperature difference treatment between day and night, plant height, lower hypocotyl length, and diameter of seedlings were +7 DIF EC level 0.5dS m -1 + D Growth was most inhibited in niconasol treatment, and leaf length, leaf width, and leaf area tended to be inhibited in the EC level 0.5dS·m -1 treatment under -7 DIF condition. In addition, the overall growth of tomato'minichal' seedlings was improved in the treatment of 1.5dS·m -1 at the EC level of -7 DIF. However hypocotyl is shown in the longest than the EC level of +7 DIF conditions 0.5dS · m -1 · m -1 processed by the EC level 1.5dS treatment of -7 DIF conditions under which promote the growth of seedlings -7 DIF conditions the length of the hypocotyl It was confirmed that it decreases. The fresh weight and dry weight of the seedlings showed a tendency to be poor in the +7 DIF condition treatment group and the -7DIF condition EC level 0.5 dS·m -1 treatment, which was judged to be due to the inhibition of growth in the treatment groups. In addition, the fresh weight and dry weight of the seedlings were the heaviest in the EC level 1.5 dS·m -1 treatment under -7 DIF condition. The compactness, an indicator of the quality of the seedlings, which indicates how short and robust the seedlings are by dividing the plant height among the above-ground buildings, was significantly higher in the EC level 1.0 and 1.5 dS m -1 treatment under -7 DIF condition, and +7 DIF. The EC level of the condition was the lowest in the 0.5dS·m -1 treatment group. The distortion rate, expressed as a standard for treatment with an EC level of 0.5 dS·m -1 under the +7 DIF condition, was highest in the treatment with an EC level of 0.5 dS·m -1 under the -7 DIF condition. Therefore, in the EC level 0.5dS m -1 treatment under the -7 DIF condition, the growth was suppressed similarly to the EC level 0.5 dS m -1 treatment under the +7 DIF condition treated with the growth regulator diniconazole. B. The fidelity indicating the quality of the seedlings was excellent at the EC level of 1.0 and 1.5 dS·m -1 under -7 DIF conditions, and the seedlings were somewhat dwarfed in this treatment. As a result, it was judged that it would be appropriate to supply the EC level of nutrient solution in the range of 1.0 dS·m -1 under -7 DIF condition in order to suppress painting and increase seedling quality while seeding tomato'minichal'.

Figure 112019000513586-pat00002
Figure 112019000513586-pat00002

Claims (6)

발아된 토마토가 식재된 플러그 트레이를 16~20℃의 명기 온도 및 23~27℃의 암기 온도가 동일한 시간으로 처리되도록 조절된 시스템에서 0.8 내지 1.2dS·m-1의 전기전도도를 갖는 양액을 플러그 트레이에 저면관수로 적용하며 22~39일 동안 육묘시키는 단계를 포함하는 토마토 플러그묘의 도장을 억제하고 플러그묘의 충실도(compactness)를 증가시키는 방법.Plug the nutrient solution having an electrical conductivity of 0.8 to 1.2 dS m -1 in a system controlled to treat the plug tray with germinated tomatoes at the specified temperature of 16 to 20 °C and the memorization temperature of 23 to 27 °C at the same time. A method of inhibiting painting of tomato plug seedlings and increasing the compactness of plug seedlings comprising the step of applying bottom irrigation to a tray and seeding for 22 to 39 days. 삭제delete 삭제delete (1) 상토가 충진된 플러그 트레이에 토마토의 종자를 파종하는 단계;
(2) 상기 토마토 종자가 파종된 플러그 트레이를 120 내지 180μmol·m-2·s-1 범위의 광도, 18 내지 22℃의 온도를 유지하는 시스템 내에서 6~18일 동안 발아시키는 단계; 및
(3) 상기 발아시킨 토마토가 식재된 플러그 트레이를 16~20℃의 명기 온도 및 23~27℃의 암기 온도가 동일한 시간으로 처리되도록 조절된 시스템에서 0.8 내지 1.2dS·m-1의 전기전도도를 갖는 양액을 플러그 트레이에 저면관수로 적용하며 22~39일 동안 육묘시키는 단계;를 포함하는 도장이 억제되고 충실도(compactness)가 증가된 토마토 플러그묘의 재배 방법.
(1) sowing seeds of tomatoes in a plug tray filled with top soil;
(2) germinating the plug tray in which the tomato seeds are sown for 6 to 18 days in a system maintaining a light intensity in the range of 120 to 180 μmol·m -2 ·s -1 and a temperature of 18 to 22°C; And
(3) The electric conductivity of 0.8 to 1.2 dS m -1 in a system controlled so that the plug tray in which the germinated tomatoes are planted is treated with a specified temperature of 16 to 20 °C and a memorization temperature of 23 to 27 °C at the same time. Applying the nutrient solution to the plug tray as bottom irrigation and seeding for 22 to 39 days; cultivation method of tomato plug seedlings with suppressed coating and increased compactness.
삭제delete 삭제delete
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임기병 외 2명. 주야간 온도차(DIF)에 따른 토마토 플러그묘의 생장과 정식후 생육. 한국생물환경조절학회 논집 6(1):34~42(1997)*
정병룡 외 1명. 육묘시의 주야간 기온이 서광 토마토의 생육 및 초기 착화 절위에 미치는 영향. 생물환경조절학회지. 8(2):75~82(1999)*

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