KR102085820B1 - Apparatus for providing complex stimulation to cells - Google Patents

Abstract

Cell complex stimulation device according to an embodiment of the present invention, the upper open housing; A chamber seat part installed in the housing such that the plurality of chambers are seated in a line and the plurality of chambers are exposed to the outside; A torsional magnetic pole portion coupled to the chamber seating portion to be pulled in opposite directions along the longitudinal direction of the chamber seating portion to twist the plurality of chambers simultaneously to provide the same torsional stimulation to the plurality of chambers; It is coupled to the chamber seating unit in cooperation with the torsional stimulating unit, so that the upper portion of the chamber seating unit opens in the width direction during operation of the torsional stimulating unit, simultaneously tensioning the plurality of chambers seated in the chamber seating unit in the widthwise direction to the plurality of chambers. A tensile stimulation portion providing the same tensile stimulation; And it is preferable to include a control unit for controlling the operation of the torsional stimulation unit, the intensity of the torsional stimulation and tensile stimulation to the plurality of chambers.

Description

Apparatus for providing complex stimulation to cells

The present invention relates to a cell complex stimulation apparatus, and more particularly, to a cell complex stimulation apparatus capable of simultaneously providing tensile and torsional stimulation to a plurality of chambers in which cells are cultured.

In general, cell research through various physical stimuli in cell biology provides important clues on pathological pathogenesis.

Studies have shown that excessive physical stimuli produce substances that cause cell death or inflammation. Proper physical stimulation, on the other hand, can aid in the production of base materials such as cell growth, differentiation and survival, and also affects the immune system by inflammatory reactants.

Various physical stimuli that the human body can easily contact include tensile force, shear force, compression force, and moment. Since torsion and tension occur simultaneously in human physics, there is a need to study cellular responses to simultaneous physical stimuli even in actual cell units.

Accordingly, the necessity of developing a device capable of observing a response pattern in a cell unit by various and complex physical stimuli is emerging.

In addition, since the pathological effects vary depending on the strength and application time of various mechanical physical stimuli, a system capable of applying stimuli under various conditions is needed.

Korean Patent No. KR0799988B1 discloses a cell culture device for stem cell culture.

It is an object of the present invention to provide a cell complex stimulation device capable of simultaneously providing tensile and torsional stimulation to a plurality of chambers in which cells are cultured.

Cell complex stimulation device according to an embodiment of the present invention, the upper open housing; A chamber seat part installed in the housing such that the plurality of chambers are seated in a line and the plurality of chambers are exposed to the outside; A torsional magnetic pole portion coupled to the chamber seating portion to be pulled in opposite directions along the longitudinal direction of the chamber seating portion to twist the plurality of chambers simultaneously to provide the same torsional stimulation to the plurality of chambers; It is coupled to the chamber seating unit in cooperation with the torsional stimulating unit, so that the upper portion of the chamber seating unit opens in the width direction during operation of the torsional stimulating unit, simultaneously tensioning the plurality of chambers seated in the chamber seating unit in the widthwise direction to the plurality of chambers. A tensile stimulation portion providing the same tensile stimulation; And it is preferable to include a control unit for controlling the operation of the torsional stimulation unit, the intensity of the torsional stimulation and tensile stimulation to the plurality of chambers.

In one embodiment of the invention, the torsional magnetic pole portion, a pair of drive panel coupled to the lower portion of the chamber seat; A rotation panel connecting a pair of drive panels; And a driving motor providing a rotational force to the rotating panel and controlling the rotation angle and the rotation direction by the control unit. In operation of the driving motor, the pair of driving panels linearly move in opposite directions by the rotating panel to the chamber. It is preferable to operate to pull the seat in different directions.

In one embodiment of the present invention, the chamber seating portion, a pair of support panels positioned to face each other with a pair of drive panels therebetween; A panel rotation shaft coupled to at least one of the pair of support panels and the housing such that at least one of the pair of support panels is rotatable at a predetermined angle; And a plurality of chamber seating units coupled to the drive panel and the support panel to be rotated at a predetermined angle with respect to the support panel while being moved in the movement direction of the drive panel during linear movement of the drive panel, each chamber facing each other. A pair of chamber seating units coupled to a pair of chamber seating units positioned to receive a torsional stimulus as the pair of chamber seating units are rotated in opposite directions by a pair of driving panels which are moved in opposite directions when the driving motor is operated. It is preferable.

In one embodiment of the present invention, the chamber seating unit, protruding in one direction, the support shaft is caught in the panel groove of the drive panel; A chamber seat protruding parallel to the support shaft; A coupling rod which protrudes to an upper portion of the chamber seat and has a coupling hole provided at one side of the chamber; A chamber seating body connecting the support shaft and the chamber seat; And a torsional rotation shaft rotatably connecting the chamber seating body to the support panel, wherein the support shaft is rotated at a predetermined angle with respect to the support panel by the torsional rotation shaft while the support shaft is moved in the movement direction of the drive panel during linear movement of the drive panel. It is preferable.

In one embodiment of the present invention, the tensile stimulation portion, the guide rail is installed in parallel with the drive panel in the lower portion of the drive panel; An operating body coupled to the driving panel and installed to be movable on the guide rail to move along the guide rail during linear movement of the driving panel; A guide body concavely curved at a predetermined curvature and coupled to an outer surface of the support panel; Positioned to be in contact with the guide groove, rotatably installed on the working body, including a tensile stimulation induction roll that is moved along the guide groove during the linear movement of the operating body, the tensile stimulation induction roll centered on the center of the guide groove Pressing the guide body away from the, the support panel is preferably stretched in the width direction while being rotated at a predetermined angle about the panel rotation axis by the force of the tension stimulation induction roll, tensioning the plurality of chambers in the width direction.

In one embodiment of the present invention, it is preferable that the housing seat, the torsional stimulation portion and the tension stimulation portion are provided in one set, and a plurality of sets are provided in the housing, and each set of the torsional stimulation portions is individually controlled by the controller. .

In one embodiment of the present invention, the chamber is spaced apart from the seating portion, the second chamber seating portion is mounted in a plurality of chambers in a row; The second chamber seat is coupled to the second chamber seat to pull the second chamber seat in opposite directions along the longitudinal direction of the second chamber seat, and simultaneously twists a plurality of chambers seated in the second chamber seat to the plurality of chambers. A second torsional stimulus for providing a stimulus; And coupled to the second chamber seating portion in cooperation with the second torsional stimulation portion, the upper portion of the second chamber seating portion is operated narrow in the width direction during operation of the second torsional stimulation portion, to provide the same compression stimulation to the plurality of chambers Further comprising a compression stimulation unit, the control unit controls the operation of the second torsional stimulation unit, it is preferable to adjust the intensity of the torsional stimulation and compression stimulation to the plurality of chambers seated in the second chamber seat.

In one embodiment of the present invention, the compression stimulation unit, the second guide rail installed in parallel with the drive panel of the second torsional stimulation unit; A second working body coupled to the driving panel and movably installed on the second guide rail to move along the second guide rail during linear movement of the driving panel; A second guide body having a second guide groove convexly curved at a predetermined curvature and coupled to an outer surface of the support panel of the second chamber seat; Located in contact with the second guide groove, rotatably installed on the second working body, comprising a compressed stimulation guide roll is moved along the second guide groove during the linear movement of the second operating body, the compression stimulation guide roll Pressing the second guide body away from the center with respect to the center of the second guide groove, and the support panel of the second chamber seat by the force of the compression stimulus induction roll is a predetermined angle around the panel rotation axis of the second chamber seat It is preferable to compress the plurality of chambers in the width direction while the upper portion of the second chamber seat portion is narrowed in the width direction while being rotated.

The present invention can provide an environment similar to the physical stimulus received by the human body by simultaneously providing a tensile stimulus and a torsional stimulus to a plurality of chambers.

In addition, the present invention can obtain a plurality of data on the cell response to tensile and torsional stimulation in each chamber in which cells are cultured in the same environment in one experiment. The data obtained by the present invention can be used as a basis for new disease treatment by deriving a pattern of cellular responses to physical stimuli.

Figure 1 schematically shows a perspective view of a cell complex stimulation device according to an embodiment of the present invention.
2 and 3 schematically show an internal perspective view of the cell complex stimulation device according to an embodiment of the present invention, viewed from different directions.
Figure 4 schematically shows a cross-sectional view of a cell complex stimulation device according to an embodiment of the present invention.
FIG. 5 schematically illustrates a plan view of a torsional stimulation unit viewed from above, in an embodiment of the present invention.
Figure 6 schematically shows a side view of the cell complex stimulation device in a side view of the torsional stimulator in one embodiment of the present invention.
7 and 8 are views for explaining the operating state of the torsional stimulation unit.
9 to 11 are views for explaining the operating state of the torsional magnetic pole portion, the tensile magnetic pole portion and the chamber seating unit.
12 is a view for explaining the tensile stimulation portion.
FIG. 13 illustrates experimental data obtained by simulating and analyzing strain occurring at both ends of the chamber when both ends of the chamber are twisted.
Figure 14 shows the average strain graph according to the horizontal position of each section of the chamber.
Figure 15 schematically shows an operating state diagram for a cell complex stimulation device according to another embodiment of the present invention.

Hereinafter, with reference to the accompanying drawings, a cell complex stimulation device according to a preferred embodiment of the present invention will be described.

● First embodiment

The present invention is an apparatus for twisting and stretching a plurality of chambers simultaneously and at the same intensity to observe the reaction of cells when tensile and torsional stimuli are simultaneously provided to the cells in the chamber in the same environment. To this end, the present invention is configured as follows. The chamber may be made of PDMS (polydimethylsiloxane) material capable of cell culture.

As shown in Figure 1 and 2, the cell complex stimulation device according to an embodiment of the present invention, the housing 110, the chamber seating portion 140, the torsional stimulation portion 120, the tensile stimulation portion 130 And a controller (not shown).

The chamber seating unit 140, the torsional magnetic pole unit 120, and the tensile magnetic pole unit 130 are configured in one set. In the housing 110, the chamber seating unit 140, the torsional magnetic pole unit 120, and the tensile magnetic pole unit 130 may be installed in a set unit. In the present embodiment, for convenience of description, it is shown divided by a set of reference numerals A and B.

Each set of torsional stimulators 120 is individually controlled by a controller (not shown). The controller (not shown) controls the operation of the torsional stimulator 120 to adjust the strength of the torsional stimulus and the tensile stimulus to the plurality of chambers 111. The intensity of the torsional stimulation and the tensile stimulation may be varied through the rotational direction and the rotational angle control of the driving motor 121 provided in each set (A, B). According to the present invention, the influence of the torsional stimulation and the tensile stimulation on the cells can be observed through the control of the driving motor 121.

As shown in FIG. 1, the housing 110 has a structure in which an upper portion of the housing 110 is opened to expose the plurality of chambers 111 to the outside. As shown in FIG. 2, the torsional magnetic pole part 120, the tensile magnetic pole part 130, and the chamber seating part 140 are installed in the housing 110.

The plurality of chambers 111 are seated on the chamber seat 140. The chamber 111 houses cells or tissues. Of course, the cells can be cultured in the chamber 111. The chamber 111 is preferably made of a stretchable material.

In the chamber seating unit 140, a plurality of chambers 111 are arranged in a line. The chamber seat 140 is installed in the housing 110 such that the plurality of chambers 111 are exposed to the outside of the housing 110. The chamber seating unit 140 simultaneously transmits the tensile stimulus and the torsional stimulus to the plurality of chambers 111 by the torsional stimulation unit 120 and the tensile stimulation unit 130.

4 and 5, the chamber seating part 140 includes a pair of support panels 141 and 142, a panel rotating shaft, and a chamber seating unit 144.

The pair of support panels are positioned to face each other with a pair of drive panels in between. In the present embodiment, for convenience of description, a pair of support panels will be referred to as being divided into a first support panel 141 and a first support panel 142.

The first a supporting panel 141 and the first b supporting panel 142 rotatably support the chamber seating unit 144. The panel rotating shaft is installed on the first b support panel 142. The panel rotating shaft is rotatably coupled to the housing 110.

The first b supporting panel 142 is rotated at a predetermined angle when a force is applied from the outside by the panel rotating shaft. In the present specification, the panel rotating shaft is shown only in the first b support panel 142, but the present invention is not necessarily limited thereto and may be installed in the first a support panel 141 with the same structure.

The plurality of chamber seating units 144 are arranged in a line to face each other on the first a support panel 141 and the first b support panel 142. Specifically, the chamber seating unit 144 installed in the first a supporting panel 141 is moved in the direction of movement of the first a driving panel 123 during the linear movement of the first a driving panel 123. It is coupled to the first driving panel 123 and the first support panel 141 to be rotated at a predetermined angle with respect to. In addition, the chamber seating unit 144 installed in the first b supporting panel 142 is moved to the moving direction of the first b driving panel 124 during the linear movement of the first b driving panel 124 to the first b supporting panel 142. It is coupled to the first b drive panel 124 and the first b support panel 142 to be rotated at a predetermined angle with respect to.

In this embodiment, each chamber 111 is coupled to a pair of chamber seating units 144 positioned facing each other. Each chamber 111 is provided with a pair of drive panels which are moved in opposite directions when the driving motor 121 is operated, thereby providing a torsional stimulus as the pair of chamber seating units 144 are rotated in opposite directions. Receive.

As shown in FIGS. 4 and 6, the chamber seating unit 144 is provided with a chamber seating body 144a, a support shaft 144b, a torsional rotation shaft 144c, a chamber seat 144d and a coupling rod 144e. Is done.

The chamber seating body 144a is rotatably coupled to each of the first a supporting panel 141 and the first b supporting panel 142 by a torsional rotation shaft 144c. The chamber seating body 144a is formed by the torsional rotation shaft 144c while the support shaft 144b is moved in the moving direction of the first a driving panel 123 / the first b driving panel 124 during the linear movement of the driving panel. The support panel 141 is rotated at a predetermined angle with respect to the first b support panel 142.

The support shaft 144b protrudes in one direction of the chamber seating body 144a. The support shaft 144b is caught in the panel groove of the drive panel.

The chamber seat 144d is coupled to an upper portion of the chamber seat body 144a. The chamber seat 144d protrudes in parallel with the support shaft 144b. The coupling rod 144e is provided in the chamber seat 144d. Coupling rod (144e) is formed to protrude to the top of the chamber seat (144d). The chamber 111 is fixed to the chamber seat 144d by the coupling rod 144e. The chamber 111 is provided with a coupling hole 111a through which the coupling rod 144e penetrates.

The torsional stimulation part 120 is coupled to the chamber seating part 140 to provide a torsional stimulation to the plurality of chambers 111 seated on the chamber seating part 140.

As shown in FIG. 5 and FIG. 6, the torsional magnetic pole part 120 includes a driving motor 121, a rotation panel 122, and a pair of driving panels 123 and 124. In the present embodiment, for convenience of description, a pair of driving panels will be referred to as the first a driving panel 123 and the first b driving panel 124.

The drive motor 121 is located at the bottom of the base. The driving motor 121 has a rotation angle and a rotation direction controlled by a controller (not shown). The rotating panel 122 is installed on the rotating shaft 121a of the drive motor 121. The rotating panel 122 is provided with a first catching shaft 122a and a second catching shaft 122b. The first catching shaft 122a and the second catching shaft 122b protrude downward from the rotating panel 122. The first locking shaft 122a is coupled to the first a driving panel 123. The second locking shaft 122b is coupled to the first b driving panel 124.

As shown in FIG. 5, the first a driving panel 123 and the first b driving panel 124 are coupled to both ends of the rotating panel 122 to face side by side. The first a driving panel 123 is coupled to the first a supporting panel 141 by the chamber seating unit 144. The first b driving panel 124 is coupled to the first b supporting panel 142 by the chamber seating unit 144. The movement direction and the distance of the first driving panel 123 and the first driving panel 124 are adjusted according to the rotation direction and the rotation angle of the rotation panel 122.

As shown in FIG. 7 and FIG. 8, the first a driving panel 123 and the first b driving panel 124 linearly move in opposite directions when the rotating panel 122 is rotated. For example, as shown in FIGS. 7 and 8, when the rotating panel 122 is rotated and the first b supporting panel 142 is pulled to the left based on the drawing, the first a driving panel 123 is the rotating panel 122. In the right direction. Alternatively, when the rotating panel 122 is rotated and the first b driving panel 124 is pulled to the right based on the drawing, the first a driving panel 123 is moved to the left by the rotating panel 122. The distance that the first a driving panel 123 / the first b driving panel 124 is moved while the rotating panel 122 pulls or pushes the first a driving panel 123 / the first b driving panel 124 is the rotating panel 122. ) Is adjusted according to the rotation angle.

Referring to FIG. 9, the operation of the chamber seating unit 144 during the operation of the first driving panel 123 will be described below.

As shown in FIG. 10, when the first a driving panel 123 is pulled in the right direction of the drawing by the rotating panel 122, the chamber seating unit 144 coupled to the first a supporting panel 141 is supported. As the shaft 144b is moved together with the first a driving panel 123 and rotated counterclockwise by the torsion rotation shaft 144c, the shaft 144b is moved in the opposite direction to the moving direction of the first a driving panel 123 (in FIG. To the left) and tilted at a predetermined angle.

Referring to FIG. 10, the operation of the chamber seating unit 144 during operation of the first b driving panel 124 will be described below.

As shown in FIG. 10, when the first b driving panel 124 is pulled to the left in the drawing by the rotating panel 122, the chamber seating unit 144 coupled to the first b supporting panel 142 is supported. As the shaft 144b is moved together with the first b driving panel 124 and rotated clockwise by the torsion rotation shaft 144c, the shaft 144b is moved in the opposite direction to the moving direction of the first b driving panel 124 (right in FIG. 10). Direction) in an inclined angle.

When the first a driving panel 123 and the first b driving panel 124 connected to one rotating panel 122 are moved in opposite directions, the chamber seating unit 144 coupled to the first a driving panel 123 and The chamber seating unit 144 coupled to the first b driving panel 124 is rotated in opposite directions to each other. As a result, the chamber 111 coupled to the pair of chamber seating units 144 is twisted as shown in FIG. 13 by a pair of chamber seating units 144 rotated in opposite directions.

The tensile stimulation part 130 is interlocked with the torsional stimulation part 120 to provide the tensile stimulation to the plurality of chambers 111.

As shown in Figure 3 to Figure 6, the tensile stimulation portion 130 is composed of a guide rail 131, the working body 132, the tensile stimulation guide roll 134 and the guide body 136. The tensile stimulation unit 130 provides a tensile stimulus to the plurality of chambers 111 at the same time. In the present embodiment, the tension stimulator 130 rotates the first b support panel 142 at a predetermined angle to tension the plurality of chambers 111 seated on the chamber seat 140.

The pair of guide rails 131 are installed at the lower side of the first a driving panel 123 and the first b driving panel 124 in parallel with the first a driving panel 123 and the first b driving panel 124.

Each of the guide rails 131 is provided with a working body 132 is linearly movable. Each operating body 132 is coupled to the first a driving panel 123 / first b driving panel 124, the guide rail 131 in the moving direction of the first a driving panel 123 / first b driving panel 124. Is moved along.

The guide body 136 is provided on the outer surface of the first b support panel 142. As shown in FIG. 12, the guide body 136 is provided with a guide groove 136a curved at a predetermined curvature.

Tensile stimulation guide roll 134 is positioned in contact with the guide groove 136a, it is rotatably installed on the working body 132. As shown in FIG. 12, the tensile stimulation guide roll 134 moves along the guide groove 136a in the same direction as the moving direction of the first b driving panel 124 with respect to the center of the guide groove 136a. Press the guide body (136).

11 and 12, the operation of the tensile stimulation unit 130 during the operation of the torsional stimulation unit 120 will be described as follows.

When the driving motor 121 is operated, when the first a driving panel 123 and the first b driving panel 124 are linearly moved in opposite directions, the chamber seating unit 144 coupled to the first a driving panel 123 and The chamber seating unit 144 coupled to the first b driving panel 124 rotates in the opposite direction and twists the chamber 111.

At the same time, the tensile stimulation part 130 is linked to the linear motion of the first b-drive panel 124. In the linear movement of the first b driving panel 124, the tensile stimulation induction roll 134 is moved in the same direction as the first b driving panel 124 by the operating body 132 which is moved together with the first b driving panel 124. do. At this time, the tensile stimulation guide roll 134 presses the guide body 136 while moving along the guide groove 136a curved at a predetermined curvature.

The first b support panel 142 is rotated at a predetermined angle around the panel rotation axis by the force of the tensile stimulation induction roll 134, and thus, the width between the first a support panel 141 and the first b support panel 142. The gap is widened. The plurality of chambers 111 connected to the first a support panel 141 and the first b support panel 142 by the respective chamber seating units 144 are provided with a force that the first b support panel 142 pulls in the Fout direction. By tension.

As described above, in the present invention, when the driving motor 121 is operated, the torsional stimulation unit 120, the tension stimulation unit 130, and the chamber seating unit 144 of the chamber seating unit 140 are simultaneously linked to each other. It works.

FIG. 13 illustrates experimental data of simulation analysis of strain occurring in chambers in each direction when both ends of the chamber are twisted at a fixed strain of 5%. FIG. 13 (a) shows a state where one end of the chamber is twisted by the process shown in FIG. 9, and FIG. 13 (b) shows a state where the other end of the chamber is twisted by the process shown in FIG.

As shown in FIG. 13, the space of the chamber is divided into three sections. The strain rate according to the x-axis position of the chamber for each section is extracted as raw data. The average strain is calculated from the average of the strains extracted from the raw data.

As shown in Figure 14, the average strain applied to the chamber is in the range of 5 W 0.3%. ㅁ 0.3% margin of error is very small, it can be seen that the applied strain and the average strain is almost the same. Here, FIG. 14 is a graph analyzing the average strain when both ends of the chamber are twisted. The x-axis of the graph shown in FIG. 14 means a horizontal position centered on the center of the chamber, and the y-axis is a value of the average strain rate for each position.

Through the simulation analysis described above, it can be seen that the strain received by the cell is determined by the value of the fixed strain of the torsion and tensile stimulus generated in the machine. When the fixed strain value set in the controller is changed, the strain value applied to the chamber is changed to change the value applied to the cell.

The present invention simultaneously provides a torsional stimulus and a tensile stimulus to the plurality of chambers 111 in the same manner as described above, thereby providing the same stimulus (tension and torsional stimulus) to the plurality of chambers 111 in the same environment. The experimental environment can be configured so that the cultured cells are subjected to the same strain rate, thereby obtaining precise data on the response pattern of the cells upon physical stimulation.

The data obtained by the present invention are data obtained under the same conditions of the change of the experimental environment (temperature, humidity) according to the experiment time and the intensity of the physical stimulus. These experimental data can be used in physiological and pathological mechanism studies by cell type, and ultimately can be used as a basis for new treatment of disease.

● second embodiment

Hereinafter, a cell complex stimulation apparatus according to a second embodiment of the present invention will be described with reference to FIG. 15.

Cell complex stimulation device according to an embodiment of the present invention, the housing 110, the torsional stimulation unit 120, the tensile stimulation unit 130, the chamber seat 140, the second torsional stimulation unit 220, compression And a magnetic pole part 230, a second chamber seating part 240, and a controller (not shown).

In the present invention, in order to avoid repetition of the description, a detailed description of the housing 110, the torsional stimulation portion 120, the tension stimulation portion 130, the chamber seat 140, the same configuration as the first embodiment described above It will be omitted. In the present embodiment, for the convenience of description, the same components as those in the above-described first embodiment are denoted by the same reference numerals.

Hereinafter, the second torsional magnetic pole portion 220, the compression magnetic pole portion 230, and the second chamber seating portion 240 having different configurations from those of the first embodiment will be described.

The second torsional magnetic pole portion 220 has the same configuration and function as the torsional magnetic pole portion of the first embodiment described above. The second chamber seat 240 has the same structure and function as the chamber seat of the first embodiment.

 However, in the present embodiment, in order to describe a process operated in conjunction with the compression stimulation unit 230, the second torsional stimulation unit 220 and the torsional stimulation unit and the chamber seating portion coupled to the compression stimulation unit 230 The second chamber seating part 240 will be referred to.

The second chamber seat 240 includes a pair of second support panels 241 and 242, a second panel rotation shaft 243, and a second chamber seat unit 244, each of which is described above. The pair of support panels 141 and 142, the panel rotating shaft 143 and the chamber seating unit 144 of the embodiment is the same.

The second torsional magnetic pole part 220 is an apparatus for pulling the second chamber seating part 240 in opposite directions to twist the second chamber seating part 240. The second torsional magnetic pole part 220 includes a second driving motor 221, a second rotating panel 222, and a pair of second driving panels 223 and 224. The second chamber seating part 240 is coupled to the pair of second driving panels 223 and 224. The pair of second driving panels 223 and 224 are connected to each other by the second rotating panel 222. The second rotating panel 222 is coupled to the second drive motor 221.

When the second driving motor 221 is operated, the second rotation panel 222 is rotated clockwise or counterclockwise, so that the pair of second driving panels 223 and 224 linearly move in opposite directions. When one of the second driving panels 223 and 224 of the second driving panels 223 and 224 is pushed in one direction, the second driving panels 223 and 224 are operated to pull the other second driving panels 223 and 224.

Due to this operating structure, when the second torsional magnetic pole portion 220 is operated, the plurality of chambers seated on the second chamber seating portion 240 may include the second chamber seating portion 240 to the second torsional magnetic pole portion 220. As a result of twisting, the same torsional stimulus is simultaneously provided.

The compression stimulation unit 230 is an apparatus that operates in conjunction with the second torsional stimulation unit 220 and provides the compression stimulation to the second chamber seating unit 240. The compression stimulator 230 includes a second guide rail 231, a second working body 232, and a compression stimulation guide roll 234.

The second guide rails 231 are installed in parallel with the second driving panels 223 and 224 under the second driving panels 223 and 224. The second operating body 232 is coupled to the second driving panels 223 and 224 and installed to be movable on the second guide rails 231 so that the second guide rails during the linear movement of the second driving panels 223 and 224. Is moved along 231.

The second guide body 236 is coupled to the outer surface of the second support panel 242. The second guide body 236 is provided with a second guide groove 236a that is convexly curved with a predetermined curvature. The second guide groove 236a is provided with a compression stimulation guide roll 234.

Compression stimulation guide roll 234 is positioned in contact with the second guide groove 236a, rotatably installed in the second working body 232, the second guide groove during the linear movement of the second working body 232 Is moved along 236a.

The compression stimulation guide roll 234 presses the second guide body 236 away from the center with respect to the center of the second guide groove 236a. At this time, the support panel 242 of the second chamber seating portion 240 is rotated at a predetermined angle around the second panel rotation shaft 243 by the force of the compression stimulus induction roll 234, the second chamber seating portion The upper portion of the 240 is operated while narrowing in the direction of the arrow Fin, compressing the plurality of chambers in the width direction of the chamber seat.

According to the present invention, an experiment for simultaneously giving a torsional stimulus and a tensile stimulus to a plurality of chambers 111 in the same manner as described above, and simultaneously performing an experiment for simultaneously giving a torsional stimulus and a compression stimulus in one experimental device, in the same environment, The stimulus may be provided to the plurality of chambers 111 to create an experimental environment in which cells cultured in the chamber receive the same strain rate, thereby obtaining precise data on the response pattern of the cells during physical stimulation. Such data can be used as medical big data in the future, and ultimately, based on the pathogenesis of the disease, it can be used as a patient-specific treatment method for each stage of degenerative disease, compared to conventional methods based on analgesic anti-inflammatory drugs and physical therapy.

The present invention can confirm gene expression and protein production caused by repetitive mechanical stress in various types of cells and tissues (eg, intervertebral discs, chondrocytes, etc.).

Afterwards, each chamber subjected to mechanical stress can be analyzed by molecular biological analysis to determine the mechanism by which cell signaling pathways caused the disease. These findings provide insight into the biological effects of repetitive and excessive mechanical stress on the progression of degenerative diseases.

The present invention described above is not limited to the above-described embodiments and the accompanying drawings, and various substitutions, modifications, and changes can be made without departing from the technical spirit of the present invention. It will be evident to those who have knowledge of.

100: cell complex stimulation device
110: housing 120: torsional magnetic pole portion
121: drive motor 122: rotary panel
123: drive panel 1a 124: drive panel 1b
130: tensile stimulation portion 131: guide rail
132: working body 134: tension stimulation guide roll
136: guide body 136: guide groove
140: chamber seat 141: first support panel
142: first support panel 143: panel rotation shaft
144: chamber seating unit
220: second torsional stimulation unit 230: compression stimulation unit
240: second chamber seat

Claims (8)

An open top housing;
A chamber seating part installed in the housing such that the plurality of chambers are seated in a line and the plurality of chambers are exposed to the outside;
A torsional magnetic pole portion coupled to the chamber seating portion to pull the chamber seating portions in opposite directions along the longitudinal direction of the chamber seating portion to twist the plurality of chambers simultaneously to provide the same torsional stimulation to the plurality of chambers;
It is coupled to the chamber seating portion in cooperation with the torsional stimulation portion, the upper portion of the chamber seating portion is widened in the width direction during the operation of the torsional stimulation portion, the plurality of chambers seated at the chamber seating portion at the same time in the width direction Tensile stimulation unit for providing the same tensile stimulation to the plurality of chambers by tension with a; And
A control unit for controlling the operation of the torsional stimulation unit to control the torsional stimulus and the tensile stimulus to the plurality of chambers
The torsional stimulation unit,
A pair of driving panels coupled to a lower portion of the chamber seat;
A rotating panel connecting the pair of driving panels; And
It provides a rotational force to the rotating panel, and includes a drive motor that is controlled by the rotation angle and rotation direction,
In operation of the drive motor, the pair of drive panel is a cell complex stimulation device, characterized in that the linear movement in the opposite direction by the rotary panel is operated to pull the chamber seat in different directions. delete The method of claim 1, wherein the chamber seat,
A pair of support panels positioned to face each other with the pair of drive panels interposed therebetween;
A panel rotation shaft coupled to at least one of the pair of support panels and the housing such that at least one of the pair of support panels is rotatable at a predetermined angle; And
And a plurality of chamber seating units coupled to the drive panel and the support panel to be rotated at a predetermined angle with respect to the support panel while being moved in a movement direction of the drive panel during linear movement of the drive panel.
Each of the chambers is coupled to a pair of chamber seating units facing each other, and the pair of chamber seating units is provided by the pair of drive panels which are moved in opposite directions when the driving motor is operated. Cell complex stimulation device characterized in that the torsional stimulation is provided as it is rotated in the opposite direction. According to claim 3, The chamber seating unit,
A support shaft protruding in one direction and caught by a panel groove of the driving panel;
A chamber seat that protrudes parallel to the support shaft;
A coupling rod which protrudes to an upper portion of the chamber seat and has a coupling hole provided at one side of the chamber;
A chamber seating body connecting the support shaft and the chamber seat; And
A torsional rotation shaft is provided to rotatably connect the chamber seating body to the support panel.
And the support shaft is rotated at a predetermined angle with respect to the support panel by the torsional rotation shaft during the linear movement of the drive panel. The method of claim 3, wherein the tensile stimulation unit,
A guide rail installed in parallel with the driving panel at a lower portion of the driving panel;
An operating body coupled to the driving panel and movably installed on the guide rail to move along the guide rail during linear movement of the driving panel;
A guide body concavely curved at a predetermined curvature and coupled to an outer surface of the support panel;
Located in contact with the guide groove, rotatably installed on the working body, including a tensile stimulation induction roll to be moved along the guide groove during the linear movement of the working body,
The tensile stimulation guide roll is pressed away from the center with respect to the center of the guide groove while pressing the guide body,
The support panel is a cell complex characterized in that the tension induces the plurality of chambers in the width direction while being opened in the width direction while being rotated at a predetermined angle around the panel rotation axis by the pressing force inducing roll Stimulation device. The method of claim 1,
The housing is provided with the chamber seat, the torsional magnetic pole portion and the tensile magnetic pole portion as a set, a plurality of sets,
Each set of torsional stimulation units is individually controlled by the control unit cell complex stimulation apparatus. The method of claim 1,
A second chamber seating part installed in the housing to be spaced apart from the chamber seating part, and having a plurality of chambers seated in a row;
The second chamber seat is coupled to the second chamber seat to pull the second chamber seat in a direction opposite to each other along the longitudinal direction of the second chamber seat, and simultaneously twists the plurality of chambers seated on the second chamber seat. A second torsional stimulation portion providing the same torsional stimulation to the plurality of chambers;
Coupled to the second chamber seating portion in cooperation with the second torsional stimulation portion, the upper portion of the second chamber seating portion is narrowed in the width direction during operation of the second torsional stimulation portion, the same compression to the plurality of chambers It further comprises a compression stimulator for providing a stimulus,
The control unit controls the operation of the second torsional stimulation unit, the cell complex stimulation device, characterized in that for adjusting the intensity of the torsional stimulation and the compression stimulation to the plurality of chambers seated in the second chamber seating portion. The method of claim 7, wherein the compression stimulation unit,
A second guide rail installed in parallel with the driving panel of the second torsional magnetic pole part; A second working body coupled to the driving panel of the second torsional magnetic pole part and movably installed on the second guide rail to move along the second guide rail during linear movement of the driving panel of the second torsional magnetic pole part;
A second guide body having a second guide groove convexly curved at a predetermined curvature and coupled to an outer surface of the support panel of the second chamber seat; And
Is positioned in contact with the second guide groove, rotatably installed on the second working body, comprising a compression stimulation guide roll is moved along the second guide groove during linear movement of the second working body,
The compression stimulus induction roll is pressed away from the center with respect to the center of the second guide groove and presses the second guide body, the support panel of the second chamber seat portion by the pressing force of the compression stimulus induction roll is the first panel And a plurality of chambers compressed in the width direction while the upper portion of the second chamber seat is narrowed in the width direction while being rotated at a predetermined angle about the panel rotation axis of the two chamber seats.

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