KR102043103B1 - Automatic genetic detector - Google Patents

Abstract

The present invention relates to an automatic gene detector, and more specifically, to an automatic gene detector which enables a rapid and convenient amplification of DNA genetic materials extracted from blood, and is configured to be made in a compact size. The automatic gene detector comprises: a module (10) in the shape of a tube; a heating unit (11) provided inside the module (10); a kit rotating device (16) formed along the central axis of the module (10); and a DNA test kit (20) releasably attached to an upper surface of the kit rotating device (16). The automatic gene detector is convenient to use by a user and can shorten the PCR amplification time in conducting genetic tests. In addition, the automatic gene detector enables respective PCR chips to accurately change to different temperatures, does not overload the device, thereby prolonging the replacement cycle of components, and facilitates a sample injection into the PCR chips and allows the sample injected in the PCR chips to move easily. Also, the automatic gene detector enables a device performing genetic tests to be automatic and smaller in size, and thus can be easily used on site and can be easily used at small to medium-sized hospitals.

Description

Fully automatic genetic detector

The present invention relates to a fully automatic gene detection device, and more particularly to a fully automatic gene detection device configured to amplify the DNA gene material extracted from the blood quickly, easily and miniaturized.

Currently, there are three types of test methods for infectious diseases: microbiological test, immunological test, and molecular biological test. Among these, microbiological test confirms the presence of viruses and the like through the culture of the sample. At this time, not only takes a lot of time, but also depending on the culture environment can not be confirmed, there is a problem that is difficult to apply to the urgent test.

In addition, the immunological test is to determine the presence of indirect factors such as antibodies of the infectious agent, the typical side flow test method has the advantage of a simple and easy to use test method, but when a new infectious agent appears, a new antibody should be developed Therefore, there is a disadvantage that the detection ability is poor at the initial stage of infection. In addition, the molecular biological test is a test that can detect the presence of direct factors, such as genetic material of the infectious agent early detection, excellent detection ability compared to immunological diagnosis, and rapid development of diagnostic reagents for new infectious agents Although possible, there is a risk of producing false positive results by inspectors or by contamination.

Accordingly, WHO has recently emphasized the need for point-of-care testing for infectious disease diagnosis, and the development of on-site inspection systems using molecular biological methods has been steadily being developed for about 10 years. .

Moreover, in general, the lab-on-a chip (LOC) technology enables a single system to carry out all molecular diagnostic procedures such as extraction of genetic material from a sample, gene amplification reaction, and diagnosis of amplification. With the emergence, researches on high-efficiency, high-sensitivity systems that can overcome the shortcomings of existing genetic diagnostic methods and enable on-site diagnosis are being actively conducted.

DNA amplification techniques required for DNA genetic testing are widely used in the life sciences, genetic engineering and medical fields, and in particular, DNA amplification techniques using PCR (Polymerase Chain Reaction) are widely used. PCR amplification is a technique for exponentially amplifying a nucleic acid by repeatedly exposing a solution containing the nucleic acid at several specific temperatures.

This prior art has evolved from using plastic tubes to chips with microchannels. This method significantly reduces the amount of sample used and the total amplification time compared to using plastic tubes, but inlet, chamber, outlet, heater, temperature Since a functional unit such as a sensor must be used in an integrated manufacturing process, there is a problem of complexity.

In addition, in the prior art, in order to speed up the reaction speed of the PCR process, a method of performing a rapid temperature conversion using a high-performance peltier device is used. However, there is a physical limitation that it is difficult to suddenly change the temperature of the peltier device, and in the process of rapidly raising and lowering the temperature of the peltier device, the peltier device is overloaded, requiring frequent replacement.

In addition, the demand for genetic testing is increasing day by day, but because the process is complex, the device must be very large, and there is a limit that takes a long time for the user to master this process, so in small and medium hospitals such as animal hospitals There are many difficulties.

Patent Registration No. 10-1420094 Patent Registration No. 10-1912942

An object of the present invention is to provide a fully automatic gene detection device that can shorten the PCR amplification time and can be easily used by a user in performing a genetic test.

It is also an object of the present invention to provide a fully automatic gene detection device capable of accurately changing a PCR chip at different temperatures and not requiring overload of the device, and thus having a long replacement cycle.

It is also an object of the present invention to provide a fully automatic gene detection device which is easy to move a sample injected into the PCR chip and the sample injected into the PCR chip.

In addition, an object of the present invention is to provide a fully automatic gene detection device that can be used in the field, small and small hospitals, and easy to use in the field by miniaturizing the size of the device for performing the genetic test and implemented in a fully automated.

In order to achieve the above object, the fully automatic gene detection device according to the present invention,

Cylindrical module 10; A heating unit 11 provided inside the module 10; And kit rotating device 16 formed on the central axis line of the module 10; It characterized in that it comprises a; DNA test kit (20) provided detachably on the upper surface of the kit rotating device (16).

In addition, in the fully automatic gene detection apparatus according to the present invention,

The heating unit 11 is composed of a first heating plate 12, a second heating plate 13, a third heating plate 14,

The kit rotating device 16 is characterized in that the circular arrangement sequentially.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The first heating plate 12, the second heating plate 13, and the third heating plate 14 are characterized in that to provide a temperature of 53 ℃, 72 ℃, and 95 ℃, respectively.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The heating unit 11 is characterized in that it is formed of a thermoelectric element.

In addition, in the fully automatic gene detection apparatus according to the present invention,

An insulating block 15 is formed between the first heating plate 12, the second heating plate 13, and the third heating plate 14.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The insulating block 15 is made of an insulating material, or the heating plate is characterized in that the empty space formed by spaced apart.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The first heating plate 12, the second heating plate 13, and the third heating plate 14 as a set, characterized in that a plurality of sets are arranged.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The heating unit 11 is characterized in that it is formed in the form of a conical surface having a predetermined inclination angle.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The kit rotating device 16 is characterized in that the rotational speed is adjusted according to the position of the DNA test kit (20).

In addition, in the fully automatic gene detection apparatus according to the present invention,

The upper surface of the kit rotating device 16 is characterized in that the fixing pin 17 for fixing the DNA test kit 20 is formed.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The DNA test kit 20 includes a sample input part 21, a reagent mixing part 22, a filter part 23, a DNA amplification part 24, and a PCR reactant storage part 25 sequentially formed in a row. It is characterized by.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The sample input part 21 of the DNA test kit 20 is characterized in that it is arranged to be located on the central axis of the kit rotating device 16.

In addition, in the fully automatic gene detection apparatus according to the present invention,

The DNA test kit 20 is characterized in that rotates about the central axis of the kit rotating device 16 in a state adjacent to the heating unit 11 in parallel.

By using the fully automatic gene detection device according to the present invention, the PCR amplification time is shortened in performing the genetic test, the user can easily use.

In addition, by using the fully automatic gene detection device according to the present invention, it is possible to accurately change the PCR chip to different temperatures, there is an effect that provides a fully automatic gene detection device with a long replacement period of the component is not overloaded. .

In addition, by using the fully automatic gene detection apparatus according to the present invention, there is an effect that the sample injection into the PCR chip and the movement of the sample injected into the PCR chip is easy.

In addition, by using the fully automatic gene detection device according to the present invention, the size of the device for performing the genetic test is reduced in size and implemented in fully automatic, it is easy to use in the field and can be used easily in small and medium hospitals.

1 is a view showing a DNA test kit coupled to the module of the fully automatic gene detection device according to the present invention.
Figure 2 is a diagram showing a module of the fully automatic gene detection apparatus according to the present invention.
Figure 3 is a side cross-sectional view of a fully automatic gene detection apparatus according to the present invention.
Figure 4 is a view showing the combination of the module and the cover of the automatic gene detection apparatus according to the present invention.
5 is a view showing an operating state diagram of a fully automatic gene detection apparatus according to the present invention.
Figure 6 is a diagram showing a DNA test kit of the automatic gene detection apparatus according to the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. Embodiment of the present invention may be modified in various forms, the scope of the present invention should not be construed as limited to the embodiments described below. This embodiment is provided to explain the present invention in more detail to those skilled in the art. Accordingly, the shape of each element shown in the drawings may be exaggerated to emphasize a more clear description.

1 is a view showing a DNA test kit of a fully automatic gene detection device according to the present invention, Figure 2 is a view showing a module of the fully automatic gene detection device according to the present invention, Figure 3 is a full automatic gene detection device of the present invention 4 shows a side cross-sectional view of a fully automatic gene detection device according to the present invention, and FIG. 5 shows a combination of a module and a cover of the fully automatic gene detection device according to the present invention. 6 is a view showing an operating state diagram of the fully automatic gene detection apparatus according to the present invention.

An embodiment of a fully automatic dielectric detector according to the present invention,

Cylindrical module 10; A heating unit 11 provided inside the module 10; And kit rotating device 16 formed on the central axis line of the module 10; It characterized in that it comprises a; DNA test kit (20) detachably provided on the upper surface of the kit rotating device (16).

The module 10 includes a heating unit 11 and a kit rotating device 16 therein, a kit rotating device 16 is formed on a central axis line of the module 10, and the kit rotating device 16 The heating unit is surrounded by a center and formed. Here, although only the cylindrical module 10 is illustrated in the present invention, the present invention is not limited thereto and may be configured in various polygonal and hemispherical shapes.

In addition, the module 10 is provided with a cover 18 formed with a sample introduction path 19, to protect the DNA test kit 20 from the external environment when performing the PCR amplification process, and to isolate from the external contaminants It plays a role.

The heating unit 11 fixedly provided inside the module 10 includes a first heating plate 12, a second heating plate 13, and a third heating plate 14. It has a configuration that is sequentially arranged circularly.

Here, referring to FIG. 2, the first heating plate 12, the second heating plate 13, and the third heating plate 14 of the heating unit 11 have temperatures of 53 ° C., 72 ° C., and 95 ° C., respectively. In general, DNA amplification kit 20 must be cycled repeatedly at 53 ° C., 72 ° C., and 95 ° C. regions to generate PCR amplification.

At this time, the first heating plate 12, the second heating plate 13, and the third heating plate 14 are manufactured using a Peltier element or the like. When the Peltier element is applied and used, the rear surface of the surface where the heat is generated is cooled due to the characteristics of the Peltier element, and the rear surface of the surface where the cooling is generated generates heat, so that the high temperature in the apparatus generated through the heating unit 11 is generated. There is no need to provide a separate cooling fan to cool the heat.

That is, since it is not necessary to provide a separate cooling fan in the apparatus, the apparatus can be miniaturized and can be easily used in the field.

In addition, an insulating block 15 may be formed between the first heating plate 12, the second heating plate 13, and the third heating plate 14. Here, the insulating block 15 is composed of an insulating material, or a space formed by spaced apart the heating plate.

This is to prevent the mutual influence between the heating plates, so that accurate heat can be transferred to the DNA test kit 20. Since the PCR amplification can be performed smoothly, the PCR amplification can be performed quickly. In addition, there is an advantage that the heating plates do not have to consume more energy to adjust their temperature.

Specifically, when the regions having the temperatures of 53 ° C, 72 ° C, and 95 ° C are adjacent to each other, PCR amplification may not be smoothly performed because the temperature cannot be delivered to the DNA test kit 20 due to the mixing of heat at adjacent areas. do. In particular, since the 53 ° C. heating plate adjacent to the 95 ° C. heating plate transfers a lot of heat from the 95 ° C. heating plate, when the DNA test kit 20 enters the 53 ° C. heating plate, the temperature is higher than 53 ° C. PCR amplification is not smooth. In addition, since the temperature is mixed in the adjacent portions of the respective heating plates, each heating plate takes more load to adjust its temperature, thereby reducing the efficiency.

In the drawings of the present invention, the first heating plate 12, the second heating plate 13, and the third heating plate 14 are provided only one by one, but this is illustrated as one cycle, but not limited thereto. A set of the first heating plate 12, the second heating plate 13, and the third heating plate 14 may include a plurality of sets of heating and cooling units, and a plurality of sets of heating units ( 11) it is a matter of course that the PCR amplification process can be performed through sufficient heating only 1 cycle.

3 and 4, the heating unit 11 may be formed in a conical surface having a predetermined inclination angle. At this time, the upper surface of the kit rotating device 16 is also formed to be inclined according to the inclination angle of the heating unit, so that the DNA test kit 20 can be disposed in parallel with the heating unit (11).

As described above, the DNA test kit 20 is rotated in a state arranged in parallel with the heating unit 11 having a predetermined inclination angle, so that the sample introduced into the sample input unit 21 of the DNA test kit 20 is gravity and centrifugal force. This makes it easy to move to the reagent mixing section 22, the filter section 23, the DNA amplification section 24, and the PCR reactant storage section 25 in the DNA test kit 20.

Kit rotation device 16 is the rotational speed is adjusted according to the position of the DNA test kit (20). This is to allow the heat of the DNA test kit 20 to be changed by the heating plate by the respective heating plates to sufficiently change the temperature of the DNA test kit 20.

In addition, the fixing pin 17 for fixing the DNA test kit 20 is formed on the upper surface of the kit rotating device 16, when the DNA test kit 20 is rotated by the kit rotating device 16, centrifugal force It serves to prevent the movement to occur by and to rotate stably.

Here, the DNA test kit 20 is formed in a rectangular shape in which the sample input unit 21, the reagent mixing unit 22, the filter unit 23, the DNA amplification unit 24, and the PCR reaction unit storage unit 25 are provided. It is formed sequentially in a line.

In FIG. 6, the shape of the DNA test kit 20 is illustrated in the form of a flat rectangular parallelepiped. However, the present invention is not limited thereto and may be formed in various shapes.

The sample input unit 21 is a space for inputting a sample sample containing a DNA gene such as blood into the DNA test kit 20. The sample input part 21 is connected to the sample input path 19 formed on the cover 18, and when the sample is introduced from the upper surface of the cover 18, the sample input part 21 passes through the sample input path 18 to the sample input part 21. Will be delivered.

The reagent mixing unit 22 serves to destroy the cell wall and the nuclear membrane by mixing the injected sample with the reagent embedded in the DNA test kit 20.

At this time, it is preferable to use a proteinase K or the like which is a proteolytic agent as a reagent for destroying the cell wall and the nuclear membrane.

In this process, the cell membrane and the nuclear membrane of the sample are destroyed and DNA in the nucleus is eluted in serum / plasma. Thus, the destroyed cell membrane, serum / plasma and eluted DNA are mixed in the sample.

In order to filter out the residues of the cell membranes and the like that are destroyed, only serum / plasma and DNA are left through the filter unit 23, and the serum / plasma and DNA are moved to the PCR amplifier unit 24, which is the next step.

Thereafter, the DNA is extracted from the PCR amplification unit 24 and the PCR amplification process is performed. After completion of the reaction, the PCR reactants are moved to the PCR reaction reservoir.

The sample inserting portion 21 of the DNA test kit 20 is positioned on the central axis of the heating portion 11 so that the sample is separated from the sample inserting portion 21 with the reagent mixing portion 22 and the filter portion 23. In this case, the DNA amplification section 24 and the PCR reactant storage section 25 can be easily moved in sequence.

In addition, the DNA test kit 20 is rotated about the central axis of the kit rotating device 16 in a state of being very adjacent to and parallel to the heating unit 11. Due to this configuration, the DNA test kit 20 facilitates receiving heat from the heating unit 11, and thus, PCR amplification is smoothly performed.

Although the present invention has been described in connection with the above-mentioned preferred embodiments, other various modifications and variations may be made without departing from the spirit and scope of the invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the invention is shown in the claims rather than the foregoing description, and all differences within the scope shall be construed as being included in the invention.

10 module 11 heating section
12: first heating plate 13: second heating plate
14: third heating plate 15: insulating block
16: kit rotation device 17: fixed pin
18: cover 19: sample injection path
20: DNA test kit 21: sample input unit
22: reagent mixing unit 23: filter unit
24: DNA amplification unit 25: PCR reaction unit storage unit

Claims (13)

Cylindrical module 10;
A heating unit 11 provided inside the module 10; And
A kit rotating device 16 formed on the center axis line of the module 10;
A DNA test kit 20 detachably provided on an upper surface of the kit rotating device 16;
It is configured to include,
The heating unit 11 is composed of a first heating plate 12, a second heating plate 13, a third heating plate 14,
Circular arrangement of the kit rotation device 16 in sequence,
The heating unit (11) is a fully automatic gene detection device, characterized in that formed in the shape of a conical surface having a predetermined inclination angle.
delete The method of claim 1,
Wherein said first heating plate (12), said second heating plate (13), and said third heating plate (14) provide temperatures of 53 ° C, 72 ° C, and 95 ° C, respectively.
The method of claim 1,
The heating unit 11 is a fully automatic gene detection device, characterized in that formed by a thermoelectric element.
The method of claim 1,
Fully automatic gene detection device, characterized in that the insulating block (15) is formed between the first heating plate (12), the second heating plate (13), and the third heating plate (14).
The method of claim 5,
The insulating block 15 is made of an insulating material, or a full automatic gene detection device, characterized in that the empty space formed by spaced apart.
The method of claim 1,
And a plurality of sets are arranged using the first heating plate (12), the second heating plate (13), and the third heating plate (14) as one set.
delete The method of claim 1,
The kit rotating device 16, the automatic gene detection device, characterized in that the rotational speed is adjusted according to the position of the DNA test kit (20).
The method of claim 1,
Fully automatic gene detection device, characterized in that the fixing pin 17 for fixing the DNA test kit 20 is formed on the upper surface of the kit rotating device (16).
The method of claim 1,
The DNA test kit 20 includes a sample input part 21, a reagent mixing part 22, a filter part 23, a DNA amplification part 24, and a PCR reactant storage part 25 sequentially formed in a row. Fully automatic gene detection device, characterized in that.
The method of claim 11,
Sample injection unit 21 of the DNA test kit 20, the automatic gene detection device, characterized in that disposed on the central axis of the kit rotating device (16).
The method of claim 1,
The DNA test kit 20, the automatic gene detection device, characterized in that rotated about the central axis of the kit rotating device 16 in a state in parallel adjacent to the heating unit (11).

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