KR102041511B1 - A cosmetic composition adsorbing fine dust comprising black pearl extract - Google Patents

Abstract

The present invention provides a cosmetic composition comprising a black pearl extract as an active ingredient.

Description

Cosmetic composition for adsorption of fine dust containing black pearl extract as an active ingredient {A COSMETIC COMPOSITION ADSORBING FINE DUST COMPRISING BLACK PEARL EXTRACT}

The present invention relates to a cosmetic composition containing the black pearl extract as an active ingredient.

External industrial contaminants such as rapid industrialization, automotive exhaust gas, heavy metal powder and fine dust from air masses from China are contaminating people's skin and are considered as a major cause of skin aging and trouble.

Yellow dust, fine dust, etc., which have been moved from China, contains a large amount of heavy metals. Heavy metals refer to heavy metal elements having a specific gravity of 4 or more such as lead, mercury, cadmium, zinc, and nickel, and heavy metals are not easily excreted when accumulated in the body. It is recognized as very dangerous because it accumulates in proteins existing in the body and has long-term side effects.

In addition, the skin has been reported to cause chemical reactions in the air to generate additional harmful substances such as nitrogen oxides (NO) to cause skin inflammation, atopy, and skin problems.

Even fine dust dust containing no heavy metals is known to contain toxic substances such as sulphate and nitrate.

Fine dust is caused by photochemical reactions in the atmosphere of car fumes and heavy metals from factory chimneys.In case of exposure to high concentrations of fine dust environment, cough, eye irritation, skin problems, lung and airway cells are inflamed. May cause

Currently, the method of removing heavy metals in the body includes a method of injecting complex forming materials such as EDTA (ethylene diamine tetraacetic acid) and BAL (British anti-lewisite) to bind the heavy metals accumulated in the body to discharge the heavy metals out of the body. Take vitamin B1, vitamin C and vitamin E to prevent heavy metals from being absorbed into your body and make it easier to drain.

However, there is no method to effectively remove heavy metals accumulated in the skin pores, and fine dust can be removed to some extent by cleansing, but the finer it is, the stronger the adsorptive power, so it penetrates deep into the pores and completely removes it with normal cleansing. It is impossible.

Meanwhile, in the cosmetic industry, many products using natural products have been developed to reduce skin irritation caused by various chemicals. Natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural ingredients has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing.

Therefore, while improving the skin stability, including extracts based on natural materials, there is a need for the development of a cosmetic composition capable of repairing the skin already damaged and excellent adsorption to fine dust.

The present invention is to solve the above-mentioned problems of the prior art, an object of the present invention is to absorb the fine dust accumulated in the skin to remove from the skin as well as a cosmetic composition excellent in skin improvement ability to repair damaged skin To provide.

According to one aspect of the invention, there is provided a cosmetic composition for adsorption of fine dust comprising the black pearl extract as an active ingredient.

In one embodiment, the cosmetic composition may further include one or more selected from the group consisting of sky feather extract, Jeju light tree extract, and sultry bread extract.

In one embodiment, the cosmetic composition may include 0.01 to 30.0% by weight of the black pearl extract based on the total weight.

In one embodiment, the cosmetic composition may further comprise a skin damage repair use.

In one embodiment, the cosmetic composition is one selected from the group consisting of softening cream, converging cream, nourishing cream, lotion, nutrition cream, massage cream, essence, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder It may be formulated as above.

The cosmetic composition according to an aspect of the present invention may contain black pearl extract as an active ingredient to adsorb and remove fine dust remaining on the skin, and may implement a repair effect on damaged skin.

In addition, when one or more components of the black pearl extract are further combined, the synergistic effects of the respective components may significantly improve the fine dust adsorption effect and the skin damage recovery effect.

It is to be understood that the effects of the present invention are not limited to the above effects, and include all effects deduced from the configuration of the invention described in the detailed description or claims of the present invention.

The terminology used herein is to select general terms that are currently widely used as possible in consideration of the functions in the present invention, but may vary according to the intention or precedent of the person skilled in the art, the emergence of new technologies and the like. In addition, in certain cases, there is also a term arbitrarily selected by the applicant, in which case the meaning will be described in detail in the description of the invention. Therefore, the terms used in the present invention should be defined based on the meanings of the terms and the contents throughout the present invention, rather than the names of the simple terms.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art, and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.

The numerical range includes the numerical values defined in the range. All maximum numerical limits given throughout this specification include all lower numerical limits as if the lower numerical limits were clearly written. All minimum numerical limits given throughout this specification include all higher numerical limitations as if the higher numerical limit were clearly written. All numerical limitations given throughout this specification will include all better numerical ranges within the broader numerical range, as the narrower numerical limitations are clearly written.

Hereinafter, examples of the present invention will be described in detail, but the present invention is not limited by the following examples.

According to one aspect of the invention, there is provided a cosmetic composition comprising the black pearl extract as an active ingredient.

The cosmetic composition can minimize the chemical formulation and replace the chemical formulation with an extract derived from nature to implement an excellent fine dust adsorption effect and skin damage recovery effect.

The "comprising as an active ingredient" is an effective amount that can exhibit a skin improving effect, for example, to remove the fine dust in the skin associated with skin adsorption or skin regeneration, inhibit the activity of inflammation factor, skin moisturizing effect, etc. It may mean to contain.

The “black pearl ” is a black butterfly ( Pinctada) Margaritifera ) is a pearl collected from margaritifera . Its beautiful color makes it expensive and rare. The production of black pearls through the production of the black butterfly clam is one of the most important industries in the South Pacific island nation.

The black pearl is mainly composed of calcium carbonate, and contains various minerals and protein components, and thus can be used for skin improvement.

In particular, since minerals and proteins contained in the black pearl play a role of purifying contaminants, the detox effect of purifying contaminants in the skin and cleaning the skin can be realized.

The black pearl extract may be included in an amount of 0.01 to 30.0% by weight based on the total weight of the composition.

When the content of the black pearl extract is less than 0.01% by weight, the skin improvement effect may not be properly implemented, and when the content of the black pearl extract is greater than 30.0% by weight, the specific gravity of other ingredients required for commercialization may be reduced, thereby lowering the product's intrinsic marketability or cost-effectiveness. Can be degraded.

On the other hand, the cosmetic composition may further include one or more selected from the group consisting of sky feather extract, Jeju light tree extract, and sultry bread extract.

Recalling “The Trichosanthes kirilowii ) ”is a vine perennial herb. The roots become thick like sweet potatoes, and the stem goes up while winding other objects with tendrils. The leaves are shifted and split into 5 ~ 7 like maple leaves with jagged teeth. Sky is a medicinal plant with excellent anticancer effect and various pharmacological effects. Triterpenoid saponin, a major component of the flying star, kills multiple cancer cells and has been known to inhibit cancer cells by more than 90%.

“ Ligustrum, Jeju Island” lucidum ) ”is also known as dangwang tree and is a kind of wild tree native to Jeju Island. Leaves are large, leathery, broad ovate, broad oval or ovate long oval, acute or obtuse, round or blunt, 3-10cm long, 2.5-4.5cm wide, with insignificant residues on the back and flat edges. 5-12mm and reddish brown with leaf veins. Fruits mature in October, and the nucleus is ovate round, 7-10mm long, ripened in purple and remains in winter. Branches have fine hairs, dense leaves, egg-shaped oval or slightly round, short in leaf disease, inflorescences with short hairs on the axial axis.

The "sawijilppang (Clematis apiifolia)" is a vine plant of ranunculales Ranunculaceae. It grows in mountains and fields and is about 3m long. It uses young leaves and stems for food and is distributed in Korea, Japan, and China. The sour bread has anti-inflammatory, analgesic diuresis, the effect of the branch, the root is Baekeuncho and is effective in low back pain and stroke. Stem is thin and is used when fever, edema, diarrhea.

The bread bread is effective in neuralgia, facial nerve palsy, stroke, migraine, muscle paralysis, rheumatoid arthritis, etc., has excellent diuretic effect is used for edema due to nephritis.

The sky feather extract, Jeju light tree extract, and oleander bread extract may exhibit excellent fine dust adsorption effect and skin damage repair effect due to the interaction between the active ingredients derived from each raw material.

The sky feather extract, Jeju light tree extract, and the oleander bread extract may be increased absorption by being applied to the skin with various components contained in each extract.

In particular, since the extract has a fine dust adsorption and skin damage recovery effect, it is possible to diversify the functionality of the cosmetic composition, maximizing the fine dust adsorption and skin damage recovery effect by the black pearl extract.

If the content deviation between the active ingredients constituting the extract is outside the above range, the synergistic effect due to the interaction between the active ingredients may not be properly implemented, so that the mixing ratio in consideration of the working environment and the quality of the final product Can be controlled appropriately.

The term "extract" refers to a solvent in which the active ingredient contained in the extract is transferred by contacting the solvent and the extract raw material under specific conditions. All may be included regardless.

For example, extracting a component dissolved in a solvent from a natural product using water or an organic solvent, and may include both a specific component of the natural product, such as obtained by extracting only a specific component such as oil.

The extract may be extracted with one or more solvents selected from the group consisting of purified water, lower alcohols having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, wherein the alcohol is 60 to 90 Ethanol at% concentration (v / v).

The active ingredient contained in the raw material may be different in the extraction ratio according to the polarity of the solvent, the ethanol has excellent selectivity in the extraction of physiologically active substances of the natural raw material to achieve the optimal skin improvement effect by the ethanol extraction Can

In particular, since water and ethanol are different polarity, the active ingredient extracted according to each polarity may be different, and the concentration of the ethanol may be appropriately controlled so that an optimal skin improvement effect may be realized. When the concentration of ethanol is greater than 90%, proper yield may not be realized, and if the concentration is less than 60%, an active ingredient exhibiting skin improvement may not be sufficiently extracted.

The extract is washed with water, washed with water, dried and pulverized, and extracted with conventional methods such as reflux circulation extraction, pressure extraction, and ultrasonic extraction for about 1 to 24 hours with a solvent having a volume of 8 to 12 times the weight of the raw material. It can be prepared by filtration. In addition, the extract may be obtained in a powder state by an additional process such as distillation under reduced pressure or freeze drying.

The extract may also include an extract that has undergone conventional purification. For example, the extract may be subjected to various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity, or affinity). It may comprise a fraction obtained through.

The cosmetic composition may be for fine dust adsorption or skin damage repair.

The "fine dust adsorption" means to remove by adsorbing harmful substances such as heavy metals remaining in the pores and the like in the skin. By treating the cosmetic composition based on the natural extracts to increase the fine dust adsorption power in the skin, it is hypoallergenic to the skin, it can eliminate the skin inflammation or skin problems that may occur when the fine dust remains.

The "skin damage repair" refers to overall skin damage such as skin elasticity, tingling, reduced moisturizing power, and reduced skin brightness due to skin irritation caused by external environment or internal factors.

The cosmetic composition may impart moisturizing, soothing, and skin regeneration effects to the damaged skin while minimizing skin damage that may occur due to skin inflammation and the like.

The cosmetic composition is one or more selected from the group consisting of flexible cosmetics, nourishing cosmetics, moisture cream, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Can be formulated.

The cosmetic composition may be formulated by conventional methods in the art. Reference may be made to the formulations disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA, for formulation of external skin preparations, and in International Cosmetic Ingredient Dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association). , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilized formulation. For example, lotion, such as a flexible lotion or a nourishing lotion; Latex, such as facial lotion and body lotion; Creams such as nutrition creams, moisture creams and eye creams; essence; Cosmetic ointments; spray; Gels; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing cream, cleansing lotion and cleansing oil; Or it may be formulated as a cleaning agent such as cleansing foam, soap, body wash, but is not limited thereto. In addition, the external preparation for the skin may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

The cosmetic composition may be appropriately blended with other ingredients within the range of not impairing the object according to the present invention, depending on the kind or purpose of use of the formulation in addition to the essential components in each formulation.

The cosmetic composition may generally include an acceptable carrier, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended, but is not limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated into ointments, pastes, creams or gels, as carrier ingredients, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc, zinc oxide or theirs Mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or mixtures thereof may be used as the carrier component, and in the case of a spray, chlorofluorohydro It may further comprise a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated into a solution or emulsion, a solvent, solubilizer, or emulsion agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan may be used.

When the cosmetic composition is formulated as a suspension, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the cosmetic composition is formulated with a soap, as a carrier component, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like Can be used.

The cosmetic composition is a fatty material, organic solvent, solubilizer, thickening agent, gelling agent, softener, antioxidant, suspending agent, stabilizer, foaming agent, fragrance which are commonly used in the industry according to the quality or function of the final product. Commonly used in surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion rod blockers, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, cosmetics It may additionally contain adjuvants commonly used in the cosmetic or dermatological arts, such as any other ingredient.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Through the following examples, the present invention will be described in more detail, but the present invention is not limited by the following examples.

Production Example  1: Preparation of Black Pearl Extract

The black pearl powder was pulverized to a size of 10 to 100 nm, deionized water was added, and microemulsified in pearl powder at 1 MPa pressure and a temperature of 50 ° C.

The pearl powder into the latex 15L in fresh water pool, removing circulating calcium (Ca ^{2 +)} was carried out for 3 hours.

Samples were taken at 15 minute intervals to measure the conductivity and calcium salt content in the pearl powder emulsion, and the solution containing the obtained calcium salt was filtered and then sterilized and sterilized to obtain pearl soluble liquid calcium. 10-fold phosphate buffer (pH 7.0) of pearl protein solution was added and homogenized.

After 40 minutes of pretreatment at 120 ° C. and 0.15 MPa environment, the mixed solution was placed in a reactor and a 1.0% neutral proteolytic enzyme was added thereto.

Enzyme catalysis was performed at 40 ° C. for 6 hours and soaked in boiling water for 15 minutes to remove the enzyme. After centrifugation, the supernatant was removed to obtain a black pearl extract solution.

Production Example  2: Preparation of Plant Extract

After washing with water, jeju loaf, and bread bread, completely dried at room temperature and pulverized to obtain a powder of 100g each.

For 100 g of each milled product, a 10-fold volume of an aqueous ethanol solution (70% mass concentration) was used as a solvent for immersion extraction at a temperature of 70 ° C for 12 hours.

The extract was filtered through a 250 mesh and 0.5 μm filter and extracted three times in the same manner for the remaining raw materials. The extract was concentrated under reduced pressure at 30 ° C. and freeze dried to obtain a solid.

Example  And Comparative example

In order to verify the skin-improving effect of the obtained extract, a sample was mixed as shown in Table 1 below to set examples and comparative examples.

As shown in Table 1, each raw material was mixed at a predetermined ratio to verify the effect of fine dust adsorption and skin damage recovery.

[Contents (parts by weight)] division Example Comparative example One 2 3 4 5 One 2 3 4 Black Pearl Extract 60 30 30 30 15 - Sky Fly Extract - 30 - - 15 60 - - 20 Jeju Tree Extract - - 30 - 15 - 60 - 20 Ice Cream Bread Extract - - - 30 15 - - 60 20

Experimental Example 1: Skin safety test In order to verify the skin safety of the samples of the Examples and Comparative Examples, MTT assay experiments on fibroblasts (HDF), melanocytes (B16F10), keratinocytes (HaCaT) was performed.

Samples of Examples and Comparative Examples were suspended in purified water by concentration (50 mg / L), respectively, and cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT) were each dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well and incubated for 48 hours at 37 ° C., 5% CO ₂ . It was.

After removing the culture medium and incubating the sample for 48 hours in a concentration-treated medium, the medium was removed and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, 50 L was added, and incubated at 37 ° C., 5% CO ₂ for 24 hours. 100 L DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and the absorbance was measured at 540 nm.

As a result, no cytotoxicity was confirmed at all concentrations of each sample. The results suggest that the extract is not only harmless to human body but also has excellent human safety.

Experimental Example  2: Inflammatory cytokine synthesis inhibitory effect test

In order to verify the skin improvement effect on the samples of the Examples and Comparative Examples, inflammatory cytokine synthesis inhibition experiment was conducted.

First, the immune cells IL-1β, IL-6 and TNF-α stimulated by poly aromatic hydrocarbons (PAHs) were treated with the samples of Examples and Comparative Examples, and then cultured for 24 hours, which were measured by qRT-PCR. .

In order to quantitatively measure the change in IL-1β, IL-6 and TNF-α gene expression by the sample, SYBR Green I (Invitrogen) was used as a method of confirming the result through the fluorescence value emitted by the product of PCR. It was.

The reaction solution was prepared by mixing 0.2 μM primer, 50M KCl, 20Mm Tris / HCl pH 8.4, 0.8mM DNTP, 0.5U Extaq DNA polymerase, 3Mm MgCl ₂ , 1 × SYBR Green.

After the reaction solution was prepared, primary denaturation was performed at 94 ° C. for 3 minutes using Linegene K (BioER), and then denaturation, annealing, and polymerization were performed at 94 ° C. for 30 seconds, 60 ° C. for 30 seconds, and 72 ° C. for 30 minutes. A total of 40 cycles were performed, and the fluorescence intensity was measured after each cycle.

The threshold cycle (Ct) value of each gene was normalized to the Ct value of β-actin, and then analyzed by comparing the amount of change in the Ct value.

The Ct value is the number of cycles when the amount of fluorescence generated by the PCR product (the number of amplified PCR products) reaches a constant reference value above the basic value. The gene expression amount was confirmed through the Ct value. It is shown in Table 2 below.

mRNA expression (multiple) division IL- 1β IL-6 TNF -α Example 1 3.6 4.2 3.7 Example 2 2.9 3.6 2.9 Example 3 3.0 3.6 3.1 Example 4 2.9 3.5 3.0 Example 5 2.3 3.1 2.3 Comparative Example 1 4.5 5.7 4.5 Comparative Example 2 4.2 5.4 4.6 Comparative Example 3 3.9 5.1 4.9 Comparative Example 4 3.8 5.3 4.7

Referring to Table 2, IL-1β, IL-6 and TNF-α expression increased by PAHs was significantly decreased after treatment of the samples of Examples 1 to 5 than that of the comparative example. Experimental Example 3: NF - kB transcriptional inhibitory effect test

In order to verify the skin-improving effect on the samples of Examples and Comparative Examples, a transcriptional inhibition experiment of NF-Kb, a transcription factor of inflammatory cytokines, was conducted.

First, a promoter region of a gene known to undergo transcription by NF-kB was cloned into a vector containing a luciferase gene and inserted into immune cells.

PAHs that induce NF-Kb transcription were activated to activate NF-kB transcription ability, and then cultured for 24 hours after treatment with the samples of Examples and Comparative Examples.

Cultured immune cells were collected directly by adding 0.1 mL lysis buffer (0.1M HEPES, pH 7.6, 1% Triton-X, 1mM DTT and 2mM EDTA) at the time of collection, and collected the cell lysate, and then 14,000 minutes at 4 ° C for 10 minutes. Cell proteins were obtained by centrifugation at rpm.

After the protein concentration was determined using the Bradford assay (Bio-Rad Laboratories), 20 μg of protein was added to the luciferase assay mixture (25 mM glycylglycine, 15 mM MgSO ₄ , 1 mg / mL BSA). , 5mM ATP and 1Mm D-luciferin (luciferin; Analytical Luminescence Laboratory) were added, and using a Monolight 2010 (Analytical Luminescence Laboratory), luminescence was measured three times for 20 seconds to calculate the average value ( Table 3).

[Luciferase Activity (Multiple)] division NF - kB Example 1 4.9 Example 2 4.2 Example 3 4.0 Example 4 4.0 Example 5 3.1 Comparative Example 1 7.6 Comparative Example 2 7.2 Comparative Example 3 7.4 Comparative Example 4 6.5

Referring to Table 3, the activity of luciferase increased by PAHs was significantly reduced after treatment with Examples 1-5 than with the samples of Comparative Examples 1-4. Experimental Example 4: Polycyclic aromatic hydrocarbon decomposition effect test

The adsorption effect of polycyclic aromatic hydrocarbons (PAH), which is a large amount of harmful air in fine dust, was tested.

After removing the fur piglets (1cm x 1cm) specimens were prepared and washed with PBS for 10 minutes. 600 ppm of Naphtalene, Acenaphthylene, Acenaphthene, Fluorene, Fluorene, Pyrene, Chrysene, and Benzopyrene after washing It was dipped in a solution (pH 11) for 2 hours and dried at room temperature.

The experimental group applied the samples of the Examples and Comparative Examples and reacted for 3 hours, the control group was applied with PBS and reacted for 3 hours.

After washing with PBS, the specimen was cut to a certain weight and then ground with a homogenizer. The pulverized tissue was dried with anhydrous sodium sulfate (Na ₂ SO ₄ ), and then polycyclic aromatic hydrocarbons were extracted from the tissue by Soxlet extraction and liquid-liquid extraction (Husain et al., 1997; Takatsuki et al., 1985), quantified by HPLC (Table 4). Quantitative results are expressed as the ratio of the control group to the experimental group.

[% of control] division naphthalene Ace
naphthalene Fluorene Fluoranthene Pyren Krissen Benzopyrene Example 1 16.1 26.4 14.6 23.3 14.1 19.3 12.9 Example 2 13.5 18.6 10.2 17.0 10.9 15.4 10.0 Example 3 13.1 17.3 9.8 16.1 10.5 15.1 9.0 Example 4 13.2 17.5 9.3 16.5 10.4 16.0 9.3 Example 5 10.3 12.3 8.0 13.9 8.6 10.9 6.7 Comparative Example 1 24.6 34.5 36.1 32.1 27.7 25.0 17.5 Comparative Example 2 27.7 38.2 39.1 37.3 30.1 28.8 19.3 Comparative Example 3 26.9 36.6 37.4 35.6 31.2 31.0 21.6 Comparative Example 4 33.7 41.1 44.0 41.1 35.4 36.9 24.0

Referring to Table 4, the polycyclic aromatic hydrocarbon was more effectively decomposed after treatment with Examples 1 to 5 than with the samples of Comparative Examples 1 to 4, and the results showed that the samples of Examples contained a large amount of fine dust and air. It can be used to effectively decompose harmful substances. Experimental Example 5: Collagen Biosynthesis Induction Test

It was confirmed whether the synthesis of collagen is promoted by the growth of fibroblasts.

Human dermal fibroblasts were dispensed in 96 well plates at a concentration of 1.0 × 10 ⁴ cells / mL, and cultured for 3 days at 37 ° C., 5% CO ₂ , and humidified conditions.

A medium containing 10 wt% of LSGS (Low Serum Growth Supplement, Kurashiki Boseki Co., Ltd.) in Medium 106S (manufactured by Kurashiki Boseki Co., Ltd.) was used at 100 µL per well.

The medium was exchanged with DMEM (Dulbecco's Modified Eagle's Medium, manufactured by Sigma), and the samples of Examples and Comparative Examples were treated at 50 mg / L and then cultured.

Samples were added without purified water as a control, ascorbic acid (50 μg / mL) was used as a positive control.

After culturing the cells for 7 days, the culture medium was collected and quantified by the enzyme-linked immunoassay (Procollagen type I c-peptide EIA Kit; manufactured by Takara Bio Co., Ltd.).

The amount of collagen in each test sample culture was calculated based on the type I collagen amount of the control group (100%), and the measurement results are shown in Table 5 below.

[% of control] division Collagen Production % ) Example 1 145.4 Example 2 151.6 Example 3 149.6 Example 4 152.0 Example 5 158.8 Comparative Example 1 111.1 Comparative Example 2 123.3 Comparative Example 3 119.0 Comparative Example 4 115.5 Positive control group 134.0

Experimental Example 6: Skin elasticity improvement effect test The cream formulation cosmetic composition of the same composition was prepared using the sample of an Example and a comparative example. Each sample was formulated with a cream of the same composition, but only the samples of Examples and Comparative Examples were different.

To measure the skin elasticity improvement effect of the cream formulation cosmetic composition, 90 subjects (20 to 40 years old) were divided into 9 groups of 10 people each, and the cream was applied twice a day to the facial area for each group 1 Application was made for months.

In order to confirm the skin elasticity improvement effect was measured using a skin elasticity measuring instrument (cutometer SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months.

The experimental results are described as the R7 value of the cutometer SEM 575, but the average value of each group is shown in Table 6 below. The R7 value represents the viscoelasticity of the skin (n = 10, p <0.05).

division R7 Example 1 0.31 Example 2 0.38 Example 3 0.36 Example 4 0.38 Example 5 0.48 Comparative Example 1 0.16 Comparative Example 2 0.14 Comparative Example 3 0.18 Comparative Example 4 0.20

Referring to Table 6, the samples of Examples 1 to 5 effectively improved the skin elasticity of the facial area. Experimental Example 7: Skin Moisturizing Effect Test

The skin moisturizing improvement effect of the cosmetics containing the sample of an Example and a comparative example was evaluated.

Samples of Examples and Comparative Examples were used to prepare cream formulation cosmetic compositions of the same composition. Each sample was formulated with a cream of the same composition, but only the samples of Examples 1 to 5 and Comparative Examples 1 to 4 were different.

In order to measure the skin elasticity improvement effect of the cream formulation cosmetic composition, 90 subjects (20 to 40 years old women) divided into nine groups of 10 people each, including the samples of Examples and Comparative Examples throughout the face for each group Cream was applied twice a day for one consecutive month.

Before starting the application, measure the skin conductivity in advance using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 ° C, 40% humidity). Skin conductivity increase rate (%) was measured and evaluated (Table 7).

division 1 week 2 weeks 4 Weeks Example 1 35 44 55 Example 2 45 49 60 Example 3 44 51 60 Example 4 47 50 61 Example 5 51 55 73 Comparative Example 1 15 20 23 Comparative Example 2 18 23 27 Comparative Example 3 13 19 23 Comparative Example 4 20 25 31

Referring to Table 7, the experimental group coated with the cosmetic composition of Examples 1 to 5 was superior in the skin conductivity increase rate compared to Comparative Examples 1 to 4. Since the skin conductivity is proportional to the skin moisture content, it was confirmed from the above results that the cosmetic product containing the sample of Example retained a higher skin moisture content than the cosmetic material containing the sample of Comparative Example.

The foregoing description of the present invention is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the invention is indicated by the following claims, and it should be construed that all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the invention.

Claims (5)

Black pearl extract; And
At least one selected from the group consisting of skyweed extract, jeju maple extract, and parsley bread extract
Cosmetic composition for adsorption of fine dust containing as an active ingredient. delete The method of claim 1,
Cosmetic composition comprising 0.01 to 30.0% by weight of the black pearl extract based on the total weight. The method according to any one of claims 1 to 3,
The cosmetic composition further comprises any one or more of skin elasticity improvement, skin tone improvement, skin inflammation relief, skin soothing, moisturizing and regeneration use The method according to any one of claims 1 to 3,
The cosmetic composition is formulated with one or more selected from the group consisting of flexible cosmetics, astringent cosmetics, nourishing cosmetics, lotions, nutrition creams, massage creams, essences, cleansing creams, cleansing foams, cleansing water, packs, sprays, and powders Composition.