KR102040877B1 - Composition for anti-diabetic, anti-aging, and increased immunity activating comprising five-colored barley - Google Patents

Abstract

The present invention relates to a pharmaceutical composition for anti-diabetic, anti-aging, and increasing immune activity comprising at least one barley seed selected from tetrastichum, green uncracked grains of barley, amethyst barley, strong blue barley, and dark cairngorm barley. By the same, a food composition for anti-diabetic, anti-aging, and increasing immune activity can significantly improve anti-diabetic, anti-aging or immune activity functions compared to using only one of conventional plain barley or colored barley.

Description

Composition for increasing anti-diabetic, anti-aging and immune activity including barley barley {COMPOSITION FOR ANTI-DIABETIC, ANTI-AGING, AND INCREASED IMMUNITY ACTIVATING COMPRISING FIVE-COLORED BARLEY}

The present invention relates to a composition for increasing anti-diabetic, anti-aging and immune activity, including five-colored barley, and more particularly, anti-diabetic, anti-aging including seeds of green barley, green barley, embroidery, Kangho Cheong barley and black water Or it relates to a food composition for increasing immune activity, and a pharmaceutical composition for treating or improving diabetes.

Barley (Barley, Hordeum vulgare L.) is mainly used for mixing in the form of barley rice, wheat wheat and bran, and is suitable for use as a staple food than other varieties and is rich in protein, calcium, and fiber in terms of nutrition and functionality. Among them, β-glucan, hydrocinnamic acid and arabinoxylan, which are functional nutrients which are not included in general grains such as rice and wheat, are contained. It is known that barley has high content of β-glucan, which is a dietary fiber, which is effective in preventing adult diseases such as preventing heart disease and reducing body fat accumulation by reducing blood cholesterol levels. In addition, barley extract is used to regulate the activity of alcohol-derived P450 family enzymes, suggesting a protective effect by reducing the occurrence of radicals and intermediates and enhancing the activity of antioxidant enzymes.

As the pigment component present in natural foods has been scientifically proved to have various bioactive effects in the human body, research on the breeding of colored crops is increasing. Black rice and black soybeans, which are representative colored crops, are rich in anthocyanin and have antioxidant, antimutagenic, anticancer and anti-inflammatory effects. In addition, colored barley strains developed in various varieties in Korea were reported to have high electron donating ability, SOD-like activity, lecithin oxidation inhibitory activity and radical scavenging ability. The content of anthocyanin pigment in black barley is reported to be higher than that of purple or blue barley. In the case of colored barley, the antioxidant content is higher than that of ordinary barley, suggesting the possibility of developing functional foods through colored barley.

Anthocyanins are water-soluble natural pigments contained in purple vegetable foods, including fruits and vegetables such as grapes and raspberries, cereals such as colored rice, purple potatoes, purple sweet potatoes, red flowers and berries, and orange, red, pink, It has various colors such as purple and blue and about 300 species exist in nature. According to the results, cyanidin and delphinidin were identified in the colored barley and reported unidentified anthocyanins. In addition, yellow, blue or black barley seeds contained delphinidin and cyanidin, and purple seeds contained pelargonidine. In addition, the proanthocyanin of colored barley extract has been reported to have an effect of increasing HL60 cell differentiation and an antimutagenic effect of Hordeum, a pigment of barley fermentation. Research into developing a variety of health functional materials using such colored barley is in progress.

Korean Patent Registration No. 10-1781109 Korean Patent Registration No. 10-1859899

An object of the present invention is to use anti-diabetic, anti-aging and immune activity using conventional white barley or colored barley using seeds of five-colored barley, including evergreen barley, green barley, embroidery barley, Kanghocheong barley, black crystal barley The present invention provides a functional food composition and a pharmaceutical composition which can be significantly improved.

It is another object of the present invention by mixing the seeds of five-colored barley, including evergreen barley, green barley, embroidered barley, Kanghocheong barley, black fertilized barley with black tea at a predetermined ratio, thereby improving the flavor and antidiabetic, anti-aging and immune activity of black tea. It is to provide a composition for black tea taebaek imparted functionality.

According to one aspect of the invention,

Provided is a food composition for increasing anti-diabetic, anti-aging or immune activity comprising at least one barley seed selected from evergreen barley, green barley, embroidery barley, Kangho cheong barley and black water barley as an active ingredient.

The barley seed may be vein.

The barley seed may be barley extract extracted by water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The food composition is green barley with respect to 100 parts by weight of barley, 65 to 85 parts by weight; Embroidery information 40 to 110 parts by weight; Kangho Cheong barley 40 to 60 parts by weight; And black water information 40 to 110 parts by weight; may include.

According to another aspect of the invention,

Provided is a pharmaceutical composition for treating or improving diabetes comprising at least one barley seed selected from evergreen barley, green barley, embroidery barley, Kangho cheong barley and black water barley as an active ingredient.

The barley seed may be barley extract extracted by water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The pharmaceutical composition is green barley 65 to 85 parts by weight based on 100 parts by weight of barley; Embroidery information 40 to 110 parts by weight; Kangho Cheong barley 40 to 60 parts by weight; And black water information 40 to 110 parts by weight; may include.

According to another aspect of the invention,

At least one barley seed selected from evergreen barley, green whole barley, embroidered barley, Kangho cheong barley and black water barley; It provides a food composition for anti-diabetic, anti-aging or increase the immune activity comprising black tea leaves.

The food composition for tea tea bags may include 100 to 500 parts by weight of black tea leaves, based on 100 parts by weight of one or more barley seeds selected from evergreen, green barley, embroidery information, Kangho Cheong barley and black water information.

The barley seeds are 100 parts by weight of barley,

65 to 85 parts by weight of green barley;

Embroidery information 40 to 110 parts by weight;

Kangho Cheong barley 40 to 60 parts by weight; And

It may include; black to about 40 to 110 parts by weight.

The barley seed may be crushed after roasting.

According to another aspect of the invention,

Preparing a barley extract by extracting at least one barley seed selected from veined barley, green barley, embroidered barley, Kangho cheong barley and black water barley with water, alcohol having 1-4 carbon atoms, or a mixed solvent thereof Provided is a method of preparing a pharmaceutical composition for treating or improving diabetes.

The barley seed may be in the form of ground powder.

Concentration may be further performed after this step.

According to another aspect of the invention,

Preparing a barley extract by extracting at least one barley seed selected from veined barley, green barley, embroidered barley, Kangho cheong barley and black water barley with water, alcohol having 1-4 carbon atoms, or a mixed solvent thereof Provided is a method for preparing a food composition comprising anti-diabetic, anti-aging or immune activity.

The barley seed may be in the form of ground powder.

Concentration may be further performed after this step.

According to another aspect of the invention,

Pulverizing at least one barley seed selected from veined barley, green barley, embroidery information, Kangho Cheong barley and black water information barley to prepare barley crushed products; It provides a method for producing a food composition for black tea tea bags for antidiabetic, anti-aging or immune activity comprising the; and mixing the barley crushed and black tea leaves.

The antidiabetic, anti-aging and immune-activating composition of the present invention is aimed at anti-diabetic, anti-aging including seeds of five-colored barley, including evergreen barley, green barrel barley, embroidery barley, Kangho Cheong barley, black water barley at a predetermined ratio And it can be remarkably improved compared with the use of conventional white barley or colored barley alone for increasing immune activity.

In addition, the composition for black tea baekbaek of the present invention by increasing the flavor of the black tea by mixing the seeds of the five-colored barley, including evergreen barley, green barrel barley, embroidered barley, Kanghocheong barley, black crystal barley with a predetermined ratio It can be given to increase diabetes, anti-aging and immune activity.

Figure 1 is a schematic diagram showing the manufacturing process of the pharmaceutical composition for increasing anti-diabetic, anti-aging and immune activity containing a five-colored barley.
2 and 3 are the results of measuring antidiabetic activity according to Experimental Example 1.
4 to 7 show the results of the immune activity test according to Experimental Example 2. FIG.
8 is a result of measuring radical scavenging ability of Experimental Example 3.
9 and 10 are results of reducing power of the five-colored barley extract of Experimental Example 4.
11 is a result of measuring the polyphenol content of the five-colored barley extract of Experimental Example 5.

As the invention allows for various changes and numerous embodiments, particular embodiments will be illustrated in the drawings and described in detail in the written description. However, this is not intended to limit the present invention to specific embodiments, it should be understood to include all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In the following description of the present invention, if it is determined that the detailed description of the related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted.

Figure 1 is a schematic diagram showing the manufacturing process of the pharmaceutical composition for treating or improving diabetes comprising a five-colored barley of the present invention. Hereinafter, with reference to Figure 1 to describe a pharmaceutical composition for treating or improving diabetes comprising a five-colored barley of the present invention.

The pharmaceutical composition for treating or improving diabetes comprising a five-colored barley of the present invention is characterized in that it comprises one or more barley seeds selected from evergreen barley, green barrel barley, embroidery barley, Kangho cheong barley and black water barley as an active ingredient.

The barley seed is preferably used in the vein state.

The pharmaceutical composition is green barley 65 to 85 parts by weight based on 100 parts by weight of barley; Embroidery information 40 to 110 parts by weight; Kangho Cheong barley 40 to 60 parts by weight; And 40 to 110 parts by weight of black water apricot.

Hereinafter, a method for preparing a pharmaceutical composition for treating or improving diabetes comprising a multicolored barley of the present invention will be described.

Method for producing a pharmaceutical composition for treating or improving diabetes mellitus of the present invention is one or more barley seeds selected from veined barley, green barrel barley, embroidery barley, Kangho cheong barley and black water barley, water, alcohol having 1-4 carbon atoms, or It may include the step of preparing a barley extract by extraction by a mixed solvent of.

The barley seed is preferably in the form of pulverized powder.

In the present invention, the five-colored barley extract may be extracted using water, an organic solvent or a mixture thereof as an extraction solvent. At this time, the type of organic solvent used and the mixing ratio of water and organic solvent are not particularly limited.

For example, the organic solvent may be one or more solvents selected from the group consisting of lower alcohols, hexane, acetone, ethyl acetate, chloroform, and diethyl ether. The lower alcohol may be an alcohol having 1 to 6 carbon atoms. For example, methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol may be used as the lower alcohol. Organic solvents include polar solvents such as acetic acid, dimethyl-formamide (DMFO) and dimethyl sulfoxide (DMSO), acetonitrile, ethyl acetate, methyl acetate, fluoroalkane, pentane, 2,2,4-trimethylpentane, and decane. , Cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene And nonpolar solvents such as benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, anneal, diethylamine, ether, carbon tetrachloride, and THF (Tetrahydrofuran).

As can be seen in the following examples, when the five-colored barley extract is extracted with an organic solvent, the five-colored barley extract is separated into a supernatant and a lower layer solution, the supernatant is an oil fraction of the five-colored barley extract, and the lower layer is a common solvent extract. Corresponding. When the lower layer is separated again by centrifugation or filter paper, it is divided into a liquid phase and a solid residue. At this time, the residue is a wax fraction of an autumnal barley extract. Therefore, the five-colored barley extract of the present invention is interpreted as a concept including both the oil fraction of the five-colored barley extract, the liquid fraction of the five-colored barley extract, and the wax fraction of the five-colored barley extract. In one embodiment, the five-colored barley extract may include any one or more selected from the group consisting of an oil fraction of the five-colored barley extract, a liquid fraction of the five-colored barley extract, and a wax fraction of the five-colored barley extract.

In one embodiment, the five-colored barley extract may be a lower alcohol extract of five-colored barley, preferably an ethanol extract of five-colored barley.

As used herein, the term 'extract' also includes fractions that additionally fractionate the extract. That is, the five-colored barley extract includes not only one obtained by using the aforementioned extraction solvent, but also one obtained by additionally applying a purification process thereto. In addition, fractions obtained by passing the extract or fraction through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the various purification methods described above are also included in the autumn barley extract of the present invention.

In one embodiment, the five-colored barley extract may be a fraction obtained by re-fractionation of the organic solvent extract of the five-colored barley into a second organic solvent. Here, the organic solvent extract of the total back includes broadly the oil fraction of the five-colored barley extract, the liquid fraction of the five-colored barley extract, and the wax fraction of the five-colored barley extract. Means a liquid fraction. Therefore, in one embodiment, the fraction re-fractionated the organic extract of the total bag with the second organic solvent may mean a fraction re-fractionated the liquid fraction of the five-colored barley extract with the second organic solvent. In another embodiment, the five-colored barley extract may be a fraction obtained by re-fractionation of the lower alcohol extract of the total bag with the second organic solvent. In another embodiment, the five-colored barley extract may be a fraction of the ethanol extract of the total bag re-fractionated with hexane. As can be seen in the following examples, the fraction of the five-colored barley extract containing a large amount of fat-soluble components of the five-colored barley shows a very excellent effect.

The term 'extract' as used herein to refer to the five-colored barley, as well as the crude extract obtained by treating the extraction solvent to the five-colored barley, as well as processed products of the five-colored barley extract. For example, the five-colored barley extract may be prepared in powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, the five-colored barley extract of the present invention is broadly meant to include a five-colored barley processed, for example, five-colored barley powder formulated to be administered to the animal itself. Although the experiment with the five-colored barley extract in the present invention, it will be expected to those skilled in the art that the desired effect can be achieved in the same form as the colored barley processed product.

On the other hand, the term 'comprising as an active ingredient' herein means containing an amount sufficient to achieve the efficacy or activity of the five-colored barley extract. In one embodiment of the present invention, the colored barley extract in the composition of the present invention is, for example, at least 0.001 mg / kg, preferably at least 0.1 mg / kg, more preferably at least 10 mg / kg, even more preferred. Preferably at least 100 mg / kg, even more preferably at least 250 mg / kg, most preferably at least 1 g / kg. Since the five-colored barley extract has no side effects on the human body even when it is excessively administered as a natural product, the upper limit of the quantity of the five-colored barley extract included in the composition of the present invention can be selected and performed by those skilled in the art within an appropriate range.

The pharmaceutical composition of the present invention may be prepared using a pharmaceutically suitable and physiologically acceptable adjuvant in addition to the active ingredient, and the adjuvant may include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, lubricants. Agents, flavors and the like can be used.

The pharmaceutical composition may be preferably formulated into a pharmaceutical composition comprising one or more pharmaceutically acceptable carriers in addition to the active ingredient described above for administration.

Formulation forms of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, nontoxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include but are not limited to natural and synthetic gums such as starch, gelatin, glucose or beta-lactose, corn sweeteners, acacia, trackercance or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.

Acceptable pharmaceutical carriers in compositions formulated in liquid solutions are sterile and physiologically compatible, including saline, sterile water, Ringer's solution, buffered saline, albumin injectable solutions, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers and bacteriostatic agents may be added as necessary. Diluents, dispersants, surfactants, binders and lubricants may also be added in addition to formulate into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

Furthermore, the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can be formulated according to each disease or component, as appropriate in the art.

The pharmaceutical composition of the present invention may be administered orally or parenterally, and in the case of parenteral administration, it may be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, or the like, and preferably, oral administration.

Suitable dosages of the pharmaceutical compositions of the present invention vary depending on factors such as the formulation method, mode of administration, age, weight, sex, morbidity, condition of food, time of administration, route of administration, rate of excretion and response to reaction, and usually The skilled practitioner can readily determine and prescribe a dosage effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention is 0.001-10 g / kg.

The pharmaceutical compositions of the present invention may be prepared in unit dosage form by formulating with a pharmaceutically acceptable carrier and / or excipient, according to methods which can be easily carried out by those skilled in the art. Or by incorporating into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension or emulsion in an oil or an aqueous medium, or may be in the form of extracts, powders, granules, tablets or capsules, and may further include a dispersant or stabilizer.

Hereinafter, the food composition for increasing anti-diabetic, anti-aging or immune activity comprising the colored barley of the present invention.

The antidiabetic, anti-aging or immune activity food composition of the present invention is characterized in that it comprises one or more barley seeds selected from evergreen barley, green barrel barley, embroidery barley, Kangho Cheong barley and black water barley as an active ingredient.

The barley seed is preferably used in the vein state.

The food composition is green barley with respect to 100 parts by weight of barley, 65 to 85 parts by weight; Embroidery information 40 to 110 parts by weight; Kangho Cheong barley 40 to 60 parts by weight; And 40 to 110 parts by weight of black water apricot.

Hereinafter, the food composition for anti-diabetic, anti-aging or immune activity enhancement tea tea bags containing the five-colored barley of the present invention.

Food composition for black tea tea bags of the present invention is one or more barley seeds selected from evergreen barley, green whole barley, embroidery information, Kangho Cheong barley and black water information barley; And black tea leaves.

In the food composition for tea tea bags, the barley seeds are 100 parts by weight of the barley, with respect to 100 parts by weight of barley, 65 to 85 parts by weight of green barley; Embroidery information 40 to 110 parts by weight; Kangho Cheong barley 40 to 60 parts by weight; And 40 to 110 parts by weight of black water apricot.

The barley seeds are preferably roasted and then crushed. More preferably, the crushed barley seeds are roughly crushed into coarse particles, not in powder form.

Hereinafter, a method for preparing a food composition for increasing anti-diabetic, anti-aging and immune activity including a colored barley of the present invention will be described.

Method for producing a food composition for increasing anti-diabetic, anti-aging and immune activity of the present invention is one or more barley seeds selected from veins of barley, green barrel barley, embroidered barley, Kangho blue barley and black water barley, water, carbon number 1-4 It may include the step of preparing a barley extract by extraction with an alcohol, or a mixed solvent thereof.

The barley seed is preferably in the form of pulverized powder.

Hereinafter, a method for preparing a food composition for black tea tea bags for increasing anti-diabetic, anti-aging or immune activity of the present invention.

First, the barley crushed product is prepared by crushing at least one barley seed selected from veined barley, green barley, embroidery information, Kangho Cheong barley and black water information.

Next, the barley crushed and black tea leaves are mixed.

At this time, it is preferable to mix 100 to 500 parts by weight of black tea leaves with respect to 100 parts by weight of one or more barley seeds selected from evergreen barley, green barrel barley, embroidered barley, Kangho cheong barley and black water barley, and more preferably 100% barley seed. It can mix 150-300 weight part with respect to a part, More preferably, 180-250 weight part with respect to 100 weight part of barley seeds.

In addition, the barley seeds are based on 100 parts by weight of the barley, 65 to 85 parts by weight of green barley relative to 100 parts by weight of barley; Embroidery information 40 to 110 parts by weight; Kangho Cheong barley 40 to 60 parts by weight; And 40 to 110 parts by weight of black water apricot.

The barley seeds are preferably roasted and then crushed. Crushed barley seeds are preferably used for tea bags, which are roughly crushed into coarse particles, not in powder form.

The food composition according to the present invention may be formulated in the same manner as the pharmaceutical composition, used as a functional food, or added to various foods. Examples of the food to which the composition of the present invention may be added include beverages, alcoholic beverages, confectionary, diet bars, dairy products, meat, chocolates, pizza, ramen noodles, other noodles, gums, ice creams, vitamin complexes, and health supplements. Etc.

The food composition of the present invention may include not only five-colored barley extract or five-colored barley crushed powder and powder as an active ingredient, but also components commonly added to foods, for example, proteins, carbohydrates, fats, nutrients, seasonings. And flavoring agents. Examples of the above carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And sugars such as conventional sugars such as polysaccharides such as dextrin, cyclodextrin and the like and xylitol, sorbitol, erythritol. As the flavoring agent, natural flavoring agents (tauumatin, stevia extract (for example rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared with a drink and a beverage, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be further included in addition to the colored barley extract of the present invention. .

The present invention provides a health functional food comprising an antidiabetic, anti-aging or immune-enhancing food composition comprising the five-colored barley extract as an active ingredient. Health functional food is a food made by adding five-colored barley extract or five-colored barley powder to food materials such as beverages, teas, spices, gums, confectionery, or encapsulated, powdered, suspensions, etc. Means to bring the effect, unlike the general medicine has the advantage that there are no side effects that can occur when taking a long-term use of the drug as a raw material. The health functional food of the present invention thus obtained is very useful because it can be consumed on a daily basis. The addition amount of the five-colored barley extract or the five-colored barley powder in the health functional food cannot be defined uniformly depending on the type of the health functional food, but it is sufficient to add it within a range that does not impair the original taste of the food. The food is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the dietary supplement of the present invention may be in the form of pills, tablets, capsules or beverages.

The present invention also provides the use of a five-colored barley extract for the manufacture of a medicament or food for antidiabetic, anti-aging or immune-enhancing activity. As mentioned above, the five-colored barley extract or the five-colored barley powder may be used for anti-diabetic, anti-aging and immuno-active purposes.

The present invention also provides an antidiabetic, anti-aging or immunoactive method comprising administering to a mammal an effective amount of a colored barley extract.

As used herein, the term "mammal" refers to a mammal that is the subject of treatment, observation or experimentation, preferably human.

As used herein, the term “effective amount” means an amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, as contemplated by a researcher, veterinarian, doctor or other clinician, Amounts that induce alleviation of the symptoms of the disease or disorder. It will be apparent to those skilled in the art that the effective amount and frequency of administration for the active ingredients of the present invention will vary depending on the desired effect. Therefore, the optimal dosage to be administered can be readily determined by one skilled in the art and includes the type of disease, the severity of the disease, the amount of active ingredients and other ingredients contained in the composition, the type of formulation, and the age, weight, general health of the patient. It may be adjusted according to various factors including the condition, sex and diet, time of administration, route of administration and the rate of secretion of the composition, the duration of treatment, and the drugs used simultaneously.

The composition comprising the five-colored barley extract as an active ingredient in the treatment method of the present invention may be administered in a conventional manner through oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. Can be administered.

Advantages and features of the present invention and methods for achieving them will be apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms, and only the embodiments are intended to complete the disclosure of the present invention, and the general knowledge in the technical field to which the present invention belongs. It is provided to fully convey the scope of the invention to those skilled in the art, and the present invention is defined only by the scope of the claims.

Hereinafter, preferred examples are provided to aid the understanding of the present invention, but the following examples are merely for exemplifying the present invention, and it will be apparent to those skilled in the art that various changes and modifications can be made within the scope and spirit of the present invention. It is natural that such variations and modifications fall within the scope of the appended claims.

EXAMPLE

Production Example  1: water extract manufacturer

Intravenous white barley (control) and colored barley, five-colored barley (Gill barley, green barrel barley, Embroidery barley, Kanghocheong barley, black crystal barley) After washing with water, it was dried finely (Ilshin Co., Yangju, Korea). 140 g of each dried sample was added to 140 ml of distilled water and placed in a 70 ° C autoclave (SJ-220A100, Sejong Co., Bucheon, Korea) for 90 minutes and centrifuged at 3000 rpm for 10 minutes (Gyrozen Co., Daejeon, Korea supernatant was taken. The supernatant was filtered with Whatman No. 2 (Whatmanplc., Kent, UK), concentrated to 70 ° C. using a rotary vacuum concentrator (R-114, Buchi Co, Flawil, Switzerland) and lyophilized.

Production Example  2: alcohol extract manufacturer

Each of the venous white barley (control) and colored barley, five-colored barley (Nile barley, green barrel, embroidered barley, Kanghocheong barley, black crystal barley) After washing with water, it was dried finely (Ilshin Co., Yangju, Korea). 140 mL of each dried sample was added to 140 mL of distilled water and extracted with shake incubator (SI-600R, JEIO THCE, Hyderabad, India) for 24 hours at 25 ℃, 170 rpm. The extract was filtered through filter paper, concentrated at 40 ° and lyophilized.

Example  1: Five-colored barley composition

A five-colored barley water extract was prepared by combining the water extracts of hulley barley, green barrel barley, embroidered barley, Kangho Cheong barley and black water barley prepared in Preparation Example 1 in a weight ratio of 2: 1.5: 2: 1: 1.

Example  2: multicolored barley composition

The five-colored barley water extract was prepared by combining the water extracts of hulley barley, green barrel barley, embroidered barley, Kangho Cheong barley and black water barley prepared in Preparation Example 1 in a weight ratio of 2: 1.5: 1: 1: 1.

Example  3: multicolored barley composition

The barley water extract was prepared by blending the water extracts of Neul barley, green barrel barley, embroidered barley, Kangho Cheong barley and black water barley prepared in Preparation Example 1 in a weight ratio of 2: 1.5: 2: 1: 2.

Example  4: barley composition

The barley water extract was prepared by combining the water extracts of Green Barley, Embroidery Barley, Kangho Cheong Barley, and Black Oyster Barley prepared in Preparation Example 1 at a weight ratio of 1.5: 2: 1: 2.

Example  5: barley composition

The barley water extract was prepared by combining the water extracts of Neul barley, Embroidery barley, Kangho Cheong barley, and black water barley prepared in Preparation Example 1 in a weight ratio of 2: 2: 1: 2.

Example  6: barley composition

The barley water extract was prepared by combining the water extracts of Neul barley, green barrel barley, Kangho Cheong barley and black water apricot prepared according to Preparation Example 1 at a weight ratio of 2: 1.5: 1: 2.

Example  7: barley composition

The barley water extract was prepared by combining the water extracts of hulley barley, green barley, embroidery barley and black water barley prepared in Preparation Example 1 at a weight ratio of 2: 1.5: 2: 2.

Example  8: barley composition

The barley water extract was prepared by combining the water extracts of hulley barley, green barrel barley, embroidery barley, and Kangho barley prepared in Preparation Example 1 in a weight ratio of 2: 1.5: 2: 1.

Example  9: barley black tea composition for tea bags

Veins of barley, green barrel, embroidery, Kangho cheong barley, black water Each washed with water and dried naturally, and roasted for 1 hour at medium heat to a brownish color. Thereafter, each roasted five-colored barley is broken into coarse particles to prevent powdering. Roasted five-colored barley is prepared by mixing barley, green whole barley, embroidery information, Kangho Cheong barley and black water information barley at a weight ratio of 2: 1.5: 2: 1: 2 to prepare a barley mixture, and the barley mixture and black tea leaf at 1: 2 By mixing in a ratio to prepare a barley black tea composition for tea bags.

Example  10: barley black tea composition for tea bags

Tea barley black tea composition was prepared in the same manner as in Example 9, except that barley mixture containing green barley, embroidered barley, Kangho Cheong barley and black water barley crushed material was used in a weight ratio of 1.5: 2: 1: 2.

Example  11: Barley Black Tea Composition for Tea Bags

Tea barley black tea composition was prepared in the same manner as in Example 9, except that barley mixture was used in which barley, embroidered berry, Kangho cheong barley and black berry lysate were mixed in a weight ratio of 2: 2: 1: 2.

Example  12: barley black tea composition for tea bags

Tea barley black tea composition was prepared in the same manner as in Example 9, except that barley mixture was prepared by mixing barley, green barley, Kangho Cheong barley and black berry barley in a weight ratio of 2: 1.5: 1: 2.

Example  13: Barley black tea composition for tea bags

Tea barley black tea composition was prepared in the same manner as in Example 9, except that barley mixture in which barley, green barley, embroidery and black water lysate were mixed at a weight ratio of 2: 1.5: 2: 2.

Example  14: Barley Black Tea Composition for Tea Bag

Tea barley black tea composition was prepared in the same manner as in Example 9, except that barley mixture was prepared using a barley mixture containing green barley, green barley, embroidered barley, and crushed barley barley in a weight ratio of 2: 1.5: 2: 1.

Table 1 below summarizes the composition of the Examples 1 to 14.

division shape Five-colored barley (relative ratio) Barley Green Barley Embroidery Information Kang Ho Cheong Barley Black water information Example 1 Water extract 2 1.5 2 One One Example 2 2 1.5 One One One Example 3 2 1.5 2 One 2 Example 4 none 1.5 2 One 2 Example 5 2 none 2 One 2 Example 6 2 1.5 none One 2 Example 7 2 1.5 2 none 2 Example 8 2 1.5 2 One none Example 9 Shredded (Black Tea Blend) 2 1.5 2 One 2 Example 10 none 1.5 2 One 2 Example 11 2 none 2 One 2 Example 12 2 1.5 none One 2 Example 13 2 1.5 2 none 2 Example 14 2 1.5 2 One none

Experimental Example

Experimental Example  One: Antidiabetic  Active measurement

Glycogen contents were measured using a modified method of Kim et al. (2017). HepG2 cells were adjusted to 1 × 10 ⁵ cells / ml, and then cultured for one day, and then the samples were treated at a concentration of 1 mg / ml and incubated for 24 hours. After 24 hours, the culture solution was removed, washed with cold PBS, and homogenized with a homogenizer and a pipette at low temperature using 200 µl sterile distilled water. The enzymes in the samples were inactivated by heating at 100 ° C. for 10 minutes and then centrifuged at 4 ° C. and 12000 rpm for 10 minutes to use the supernatant for the experiment. Proteins were quantified by the Bradford assay method and glycogen content was measured using a glycogen assay kit (Abcam, Cambridge, UK). Glycogen standard and 50 µl of cell extract were dispensed into 96-well, and 50 µl of hydrolysis buffer and 2 µl of hydrolysis enzyme were added. After reacting for 30 minutes at room temperature, 50 μl of reaction mix (46 μl of development buffer, 2 μl of development enzyme mix, 2 μl of oxiRed probe) was dispensed into all wells, blocked with light, and reacted at room temperature for 30 minutes, followed by a microplate reader (Biochrom Ltd). , Holliston, MA, USA) was used to measure the absorbance at 570 nm, and the results are shown in FIGS. 2 and 3.

According to FIG. 2, the glycogen content of the water extract was relatively high in barley barley in each experimental group, but the glycogen content was remarkably high in the composition treatment group of Examples 1 to 3 containing all five colored barleys. . Therefore, the colored barley extract of the present invention was confirmed that the anti-diabetic function is significantly superior to each of the colored barley extract.

According to Figure 3, Examples 4 to 8 of the composition treatment group of Example 1 to 3, including all the barley, green barley, embroidery information, Kangho Cheong barley and black water information, except one of the five types of barley Comparing the glycogen content of the composition treatment group, except for Example 5 except green barley, the glycogen content was significantly lower than that of the composition treatment groups of Examples 1 to 3. In addition, the composition treatment group of Example 5 also showed a lower glycogen content than the composition treatment group of Examples 1 to 3. That is, it can be seen that the composition containing all five colors of barley has a significant effect on anti-diabetes, and when any one of the barley is excluded, the anti-diabetic effect is very low.

Experimental Example  2: immune activity test

Raw 264.7 was used to examine the effects on cell viability, NO production, TNF-α, and cytokine (cytokine) production of COX-2, and are shown in FIGS. 3 to 6, respectively.

4 shows a comparison of cell viability. According to the results of comparing the effect of the composition of Example 2 and Example 3 on the survival rate of macrophages, the survival rate was increased during the treatment of Examples 2 and 3, and in particular, the composition of Example 3 significantly increased the survival rate appear.

Figure 5 is a comparison of the effect of the composition of Example 2 and Example 3 on NO production in macrophages. This showed a significant decrease in both colored barley Examples 2 and 3.

6 and 7 show the results of comparing the effects of the compositions of Examples 2 and 3 on TNF-α and COX-2 production of macrophages, respectively. According to this, there was a significant decrease in the compositions of Examples 2 and 3. Therefore, the mixture of five-colored barley is judged to have an excellent effect on anti-aging activity and suppression of immune activity.

Experimental Example  3: Radical Scavenging power  Measure

ABTS radical scavenging ability was used by modifying the method of Re et al. (1999) and Arano et al. (2001). 800 μl of ABTS solution mixed with 2.45 mM potassium persulfate in a 7 mM ABTS solution for about 24 hours in the dark was mixed with 200 μl of the sample, reacted for 6 minutes in the dark, and the absorbance was measured at 734 nm. Indicated. The result shows the ratio of scavenging activity of the radicals as a percentage (%) by comparing the extract addition group with the control group.

According to this, the ABTS radical scavenging ability of the ethanol extract of five-colored barley showed high activity at 1000 ㎍ / ml concentration. Especially, barley barley (81%) and green barley (83.86%) showed the highest activity, followed by Kangho Cheong barley (74.57%), Embroidery barley (73.85%), black water barley (67.43%), and white barley barley (55.81%). Was high and these figures showed a significant difference.

Experimental Example  4: Determination of reducing power of five-colored barley extract

The results of measuring the reducing power of the five-colored barley ethanol extract is shown in Figure 9, the results of measuring the reducing power of the five-colored barley water extract is shown in Figure 10.

According to Figure 9, the reducing power in the ethanol extract showed the highest activity of the barley. Next, Kang Ho-cheong and Embroidery Bori showed similar activities. Next, green barley, white barley, black fertilized barley showed high activity in order.

According to Figure 10, the reducing power in the water extract showed the highest activity at 1000 ㎍ / ㎖ barley was similar to green barley at 500 ㎍ / ㎖. In addition, the black water cherries showed high activity at 1000 ㎍ / mL, followed by white barley, Kangho Cheong barley and green barley. Embroidery information showed the lowest activity

Experimental Example  5: Determination of Polyphenol Content in Five-Colored Barley Extract

The results of measuring polyphenol content of the five-colored barley extract are shown in FIG. 11.

According to this, green barley was about 3 times higher in polyphenol content than white barley (control). In addition, the five-colored barley extract composition of Example 1 (Gold barley: Green barrel barley: Embroidery information: Kangho Cheong barley: Black water information: = 2: 1.5: 2: 1: 1) was more than four times higher polyphenol content.

Experimental Example  6: Sensory Evaluation of Barley Black Tea for Tea Bags

Thirty evaluation agents were selected and used for each bag of tea, barley tea, white barley (control), evergreen barley, green barley, embroidered barley, Kangho cheong barley and embroidered barley, respectively prepared according to Examples 9-14. Barley black tea was put into each tea bag and put in a tea bag for 1 minute in water of about 90 ℃, and sensory evaluation was performed on the color, row, taste and overall acceptability by the nine-point scale method. One point for each test item is very bad or low, and nine points are very good or strong. The final response scores were summed and averaged, and the results are shown in Table 2 below.

division color incense flavor Overall preference Example 9 7.3 7.7 8.8 8.1 Example 10 7.1 6.1 6.4 6.4 Example 11 7.8 7.3 6.7 6.2 Example 12 6.4 6.8 7.9 6.2 Example 13 7.3 6.5 7.8 6.8 Example 14 6.5 7.5 6.1 5.8 Barley 6.7 5.5 3.5 3.5 Green Barley 6.4 5.3 2.7 2.6 Embroidery Information 8.1 6.9 4.6 5.5 Kang Ho Cheong Barley 7.5 5.2 2.7 3.1 Black water information 7.8 7.3 4.5 5.4

According to this, barley black tea containing only one barley showed a relatively low acceptability compared to Examples 10 to 14 including four barley, the overall acceptability of barley black tea for tea bags containing all five colors of barley 4 It was confirmed that the evaluation was relatively high compared to Examples 10 to 14 including the species.

As described above, embodiments of the present invention have been described, but those skilled in the art may add, change, delete, or add elements within the scope not departing from the spirit of the present invention described in the claims. The present invention may be modified and changed in various ways, etc., which will also be included within the scope of the present invention.

Claims (19)

Contains barley seeds of evergreen barley, green whole barley, embroidered information barley, Kangho Cheong barley and black water information barley as active ingredients,
The barley seed is based on 100 parts by weight of the barley,
75 parts by weight of the green barley;
50 to 100 parts by weight of the embroidery information;
50 parts by weight of the strong blue barley; And
50 to 100 parts by weight of the black water information;
The barley seed is an anti-diabetic food composition, characterized in that the extract extracted by water. The method of claim 1,
The barley seeds are anti-diabetic food composition, characterized in that the vein. delete delete Contains barley seeds of evergreen barley, green whole barley, embroidered information barley, Kangho Cheong barley and black water information barley as active ingredients,
The barley seed is based on 100 parts by weight of the barley,
75 parts by weight of the green barley;
50 to 100 parts by weight of the embroidery information;
50 parts by weight of the strong blue barley; And
50 to 100 parts by weight of the black water information;
The barley seed is a pharmaceutical composition for treating diabetes, characterized in that the extract extracted by water. The method of claim 5,
The barley seed is a pharmaceutical composition for treating diabetes, characterized in that the vein. delete delete Barley seeds of evergreen barley, green whole barley, embroidered barley, Kanghocheong barley and black water barley; And black tea leaves;
The barley seed is based on 100 parts by weight of the barley,
75 parts by weight of the green barley;
50 to 100 parts by weight of the embroidery information;
50 parts by weight of the strong blue barley; And
50 to 100 parts by weight of the black water information;
The barley seed is an antidiabetic black tea tea food composition, characterized in that the extract extracted by water. delete delete The method of claim 9,
The barley seed is a food composition for anti-diabetic black tea tea bag, characterized in that it is crushed after roasting. And extracting barley seeds of veined barley, green barley, embroidered barley, Kangho Cheong barley and black water barley with water to produce barley extract,
The barley seed is based on 100 parts by weight of the barley,
75 parts by weight of the green barley;
50 to 100 parts by weight of the embroidery information;
50 parts by weight of the strong blue barley; And
50 to 100 parts by weight of the black water information, characterized in that it comprises a, a method for producing a pharmaceutical composition for treating diabetes. The method of claim 13,
The barley seed is a method for producing a pharmaceutical composition for treating diabetes, characterized in that the powder form. The method of claim 13,
Method of producing a pharmaceutical composition for treating diabetes, characterized in that to perform further after the step of concentrating. And extracting barley seeds of veined barley, green barley, embroidered barley, Kangho Cheong barley and black water barley with water to produce barley extract,
The barley seed is based on 100 parts by weight of the barley,
75 parts by weight of the green barley;
50 to 100 parts by weight of the embroidery information;
50 parts by weight of the strong blue barley; And
50 to 100 parts by weight of the black water apricot; characterized in that it comprises a, a method for producing an antidiabetic food composition. The method of claim 16,
The barley seed is a method of producing an antidiabetic food composition, characterized in that the powder form. The method of claim 16,
Method of producing an anti-diabetic food composition, characterized in that for performing the further step after the step of concentrating. Preparing barley crushed by crushing barley seeds of veined barley, green barley, embroidered barley, Kangho blue barley and black water barley; And
Comprising; mixing the barley crushed and black tea leaves;
The barley seed is based on 100 parts by weight of the barley,
75 parts by weight of the green barley;
50 to 100 parts by weight of the embroidery information;
50 parts by weight of the strong blue barley; And
50 to 100 parts by weight of the black water information Lee; characterized in that it comprises a, method for producing a food composition for anti-diabetic black tea tea bags.

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