KR102026781B1 - Method of manufacturing spirulina having increased selenium and use thereof - Google Patents
Method of manufacturing spirulina having increased selenium and use thereof Download PDFInfo
- Publication number
- KR102026781B1 KR102026781B1 KR1020170047330A KR20170047330A KR102026781B1 KR 102026781 B1 KR102026781 B1 KR 102026781B1 KR 1020170047330 A KR1020170047330 A KR 1020170047330A KR 20170047330 A KR20170047330 A KR 20170047330A KR 102026781 B1 KR102026781 B1 KR 102026781B1
- Authority
- KR
- South Korea
- Prior art keywords
- spirulina
- present
- selenium
- anhydride
- sodium selenate
- Prior art date
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Abstract
The present invention relates to a method for producing spirulina with enhanced selenium content and the use of spirulina with enhanced selenium content, and specifically, the present invention relates to a spirulina with increased selenium content comprising culturing in a medium containing sodium selenate anhydride. It relates to a production method. Spirulina produced by the method of the present invention contains a very high content of selenium and has an excellent antioxidant activity, and thus can be widely used in the raw materials of health functional foods, medicines and cosmetics.
Description
The present invention relates to a method for producing spirulina with enhanced selenium content and the use of spirulina with enhanced selenium content.
Marine microalgae grow fast and are relatively easy to cultivate, which means that pharmaceuticals and health foods, dyes and cosmetics (carotenoids, biliprotein, etc.), refined chemicals (bioflocculant, polyols, carbohydrates, etc.), biofuels (oil, hydrocarbons, etc.) It is a good biological material that can be used in a variety of fields, and is widely used in the environment (water quality and soil purification) and agriculture (biological fertilizer, growth promoter, etc.).
On the other hand, Spirulina (Arthrospira) is a prokaryote belonging to cyanobacteria, and more than 70% of the cells contain high protein and can be used as a protein source, carotene (carotene),
Spirulina is also an excellent source of vitamin B12 (Estrada et al. 2001), which has immuno-promoting effects and antioxidant effects (Xue et al. 2002). High protein biomass, arid, suitability for semi-arid areas, and the possibility of cultivation in saline water are advantages of spirulina.
On the other hand, selenium is one of the essential trace elements in our body, and it is a component of the antioxidant enzyme (glutathione peroxidase), which prevents oxidative damage and protects cells and cell membranes from free radicals caused by fat peroxidation. Cells contain this enzyme, so selenium is a very important factor for intracellular antioxidants. In addition, it is known to be a powerful antioxidant that removes free radicals that cause cellular damage and an anticancer effect that inhibits the production of carcinogens and promotes detoxification. It is known to function to prevent the immune system by maintaining globulin at a constant level and to bind the harmful heavy metals such as mercury, cadmium and lead absorbed in the body, and to inactivate the heavy metals and release them to the body.
However, lack of selenium in soil or water can cause Keshan and Cassinbeck disease, and the World Health Organization (WHO) recommends intake of 50 to 200 ㎍ / day, while the average intake of Koreans is 40㎍ / day. It is lacking and can not be synthesized in the body is an important nutrient must be obtained through food. In recent years, selenium deficiency has been reported in some parts of Europe and the United States, and in Europe, the level of selenium in soils has been lowered in some countries. .
In addition, selenium is found in the organic and inorganic divalent form in nature, the inorganic form selenium is recognized as a mineral in the body's metabolism and is discharged to the body immediately after being absorbed. The organo selenium is recognized in the form of selenomethionine in the same way as methionine, an amino acid, and has excellent absorption in the body.
Therefore, the present inventors completed the present invention by establishing a method of enhancing the selenium content in spirulina as a method for further enhancing the physiological activity of spirulina.
It is therefore an object of the present invention to provide a method for producing spirulina with increased selenium content.
Another object of the present invention is to provide spirulina with increased selenium content.
Another object of the present invention is to provide an antioxidant food composition comprising spirulina having increased selenium content as an active ingredient.
Still another object of the present invention is to provide a cosmetic composition having antioxidant activity including spirulina having increased selenium content as an active ingredient.
In order to achieve the above object, the present invention provides a method for producing spirulina with increased selenium content, including culturing in a medium containing sodium selenate anhydride.
In one embodiment of the present invention, the sodium selenate anhydride may be added to the medium at a concentration of 10 mg / L ~ 100 mg / L.
In one embodiment of the present invention, the sodium selenate anhydride may be injected into the medium at a flow rate of 0.30ml / min ~ 0.40ml / min for 40 to 50 hours.
In one embodiment of the present invention, the culturing may be to cultivate under the conditions of 28 ~ 32 ℃ temperature, 150 μmol / ㎡ / s light quantity and photoperiod Day: Night is 16: 8 have.
The present invention also provides spirulina with increased selenium content produced by the process of the invention.
In one embodiment of the present invention, the selenium content may be contained in spirulina 250mg / kg ~ 300mg / kg by weight.
In another aspect, the present invention provides an antioxidant food composition comprising spirulina with increased selenium content as an active ingredient.
In another aspect, the present invention provides a cosmetic composition having an antioxidant activity containing spirulina having increased selenium content of the present invention as an active ingredient.
The present invention relates to a method for producing spirulina with enhanced selenium content and the use of spirulina with enhanced selenium content, and specifically, the present invention relates to a spirulina with increased selenium content comprising culturing in a medium containing sodium selenate anhydride. It relates to a production method. Spirulina produced by the method of the present invention contains a very high content of selenium and has an excellent antioxidant activity, and thus can be widely used in the raw materials of health functional foods, medicines and cosmetics.
Figure 1 shows the results of analyzing the growth rate of spirulina according to the sodium selenate anhydride treatment concentration in one embodiment of the present invention.
Figure 2 shows the results of comparative analysis of spirulina production yield in the group treated with sodium selenate anhydride and the untreated control.
Figure 3 shows the results of analyzing the total polyphenol content in the group treated with sodium selenate anhydride and the untreated control.
Figure 4 shows the results of analyzing DPPH radical scavenging ability in the group treated with sodium selenate anhydride and the untreated control group.
The present invention relates to a method for producing spirulina with enhanced selenium content and a functional food composition comprising spirulina with enhanced selenium content as an active ingredient.
The inventors have established a method for producing spirulina fortified with selenium useful for humans as a method for further improving the functionality of spirulina, and thus the present invention includes culturing in a medium containing sodium selenate anhydride, thereby increasing the selenium content. It is characterized by providing a method of producing spirulina.
In particular, sodium selenate anhydride in the method according to the present invention is added to the medium at a concentration of 10mg / L ~ 100mg / L, the medium is injected for 40-50 hours at a flow rate of 0.30ml / min ~ 0.40ml / min.
In addition, the culture of spirulina in medium containing sodium selenate anhydride was incubated under the conditions of a temperature of 28-32 ° C., light quantity of 150 μmol /
The spirulina obtained by culturing under the above conditions was confirmed that the selenium content is significantly enhanced, in particular, the inventors confirmed that the selenium content is contained in the 250 mg / kg ~ 300 mg / kg based on the spirulina weight.
Therefore, the present invention can provide spirulina with increased selenium content with the provision of spirulina with increased selenium content.
Furthermore, the present inventors analyzed the antioxidative activity of spirulina with increased selenium content of the present invention. As a result, sodium selenate anhydride in the total polyphenol content analysis was performed in the untreated and treated groups of sodium selenate anhydride. The treatment group was increased by about 12% compared to the control group, and DPPH radical scavenging ability was increased by about 2.6 times compared to the control group.
Therefore, the spirulina with increased selenium content of the present invention has very good antioxidant activity, and thus can be very useful as a health functional food or a functional cosmetic material.
The present invention can provide a functional food composition having an antioxidant activity containing spirulina having increased selenium content according to the present invention as an active ingredient.
The food composition according to the present invention may be prepared in the form of a composition by mixing with a known active ingredient known to have an antioxidant effect, and may further include a food acceptable additive. The food composition of the present invention includes compositions for all types of foods, such as functional foods, nutritional supplements, health foods and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art. For example, the health food can be prepared in the form of gum, vitamin complex, tea, juice and drink containing selenium-enhanced spirulina of the present invention, granulated food composition comprising selenium-enhanced spirulina as an active ingredient It may be consumed in powdered form, encapsulated or powdered. Functional food compositions of the present invention may include ingredients that are commonly added in the manufacture of food, and may include, for example, proteins, carbohydrates, fats, nutrients and seasonings.
Food additives as defined in the present invention include food additives customary in the art, such as flavoring agents, flavoring agents, coloring agents, fillers, stabilizers and the like. The health beverage composition of the present invention has no particular limitation on the liquid component except for containing the spirulina as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the natural carbohydrates described above include, for example, conventional sugars such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and xylitol, sorbitol, Sugar alcohols such as erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extracts (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) are advantageously used. Can be. The proportion of said natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention. In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.
Furthermore, the present invention can provide a cosmetic composition having antioxidant activity including spirulina having increased selenium content as an active ingredient.
The cosmetic composition according to the present invention can be used for the purpose of improving the skin condition worsened by the action of reactive oxygen species, specifically, damage caused by oxidation of the skin, for example, spots (brown spots), freckles, It can prevent or improve symptoms such as skin cracking, UV damage (sunburn), wrinkles on the surface of skin and decrease elasticity, so it can be used to suppress skin aging, maintain skin elasticity, improve skin wrinkles, and whiten skin. Can be used. In addition, spirulina of the present invention has an antioxidant activity, it can be very useful to maintain the quality of cosmetics by preventing oxidation of the cosmetics themselves. Selenium-enhanced spirulina of the present invention may be included in the cosmetic composition having an antioxidant activity in an amount of 0.01 to 40% by weight, preferably 0.01 to 20% by weight. When the content is less than 0.01% by weight, the antioxidant activity may be insignificant, and when the content exceeds 40% by weight, the antioxidant effect may be relatively low compared to the input concentration.
In the cosmetic composition of the present invention, the selenium-enhanced spirulina may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form may include, but are not limited to, for example, cosmetics, creams, lotions, baths and the like. Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited and include, for example, water, alcohols, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin and the like.
Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are intended to illustrate the present invention more specifically, but the scope of the present invention is not limited to these examples.
<Example 1>
Cultivation and Production of Selenium-Enhanced Spirulina
<1-1> Spirulina Mass Culture
In order to produce selenium-enriched spirulina, spirulina was first cultured under the following conditions, and mass culture was performed using spirulina stock culture.
That is, 50 μl of NaNO 3 , KH 2 PO 4 in a culture vessel containing 100 ml of sterile sterilized spirulina cultured under the conditions of Table 1 for the preparation of spirulina or starch culture solution. 50 μl,
Wherein the composition of the Zarrouk medium is as shown in Table 2, in the case of 5L medium production, NaHCO 3 84 g, K 2 HPO 4 2.5g, NaNO 3 12.5g, K2SO 4 5g, NaCl 5g, MgSO 4 * 7H 2 O 0.05g, CaCl 2 0.2g, FeSO 4 * 7H 2 O 0.05g, Na 2 EDTA 0.4g, Solution A5 5ml, Solution B6 5ml was added to 5L of distilled water and aerated for about 2 days to melt and use.
2L of Zarrouk medium was inoculated with Spirulina grown in stock inoculated with 10% (200ml) of 2L vessels for one week, and a week later, 5L of Zarrouk medium was inoculated with 10% (500ml) of spirulina grown in 2L vessels. 11 days later, spirulina was inoculated with 10% (1500 ml) of 15 L container of Spirulina grown in a 5 L container in 15 L of Zarrouk medium, followed by culturing for 11 days.
<1-2> Growth Rate Analysis of Spirulina According to Sodium Selenate Anhydride Concentration
Sodium selenate anhydride (Na 2 SeO 4 ) was used as a material for improving the selenium content of spirulina, and before then, the effect of the growth rate of spirulina on the concentration of sodium selenate anhydride was analyzed. To this end, 200 ml of sterile seawater was added to the Erlenmeyer flask, 13.2 μL / 100 ml of F / 2 medium was added, and sodium selenate anhydrous (Na 2 SeO 4 ) was added at each concentration of 0, 10, 50, 100, It was added to the Erlenmeyer flask so as to have a concentration of 200 mg, and to dissolve it, a magnetic bar was added and stirred for 10 minutes in a stirrer at 100 rpm. After inoculating spirulina into the seawater with sodium selenate anhydride added in concentrations of 10%, L (Light): D (Dark) was obtained at a culture temperature of 28 ~ 32 ℃ and light quantity of 150 μmol / ㎡ / s. Incubated for 11 days under the condition of 16: 8. After 11 days, the spirulina relative growth rate was measured by using a flour meter, and the final spirulina growth rate was expressed as% of the control group without adding sodium selenate anhydride.
As a result, as shown in Figure 1, the growth rate of the spirulina according to the concentration of sodium selenate anhydride was shown to be lowered, especially when the addition of 10mg / L compared to the control showed a growth rate of 94%, 200mg / L In the case of addition, most of them appeared to have died. Therefore, through these results, the inventors found that it is preferable not to treat sodium selenate anhydride in excess of 200 mg / L concentration.
<1-3> Selenium-enhanced spirulina production
15 L of Zarrouk medium was inoculated with spirulina in an amount of 10%, incubated for 11 days, and then 1 L of a volumetric flask was placed in a 1 L Erlenmeyer flask, and sodium selenate anhydrous (Na 2 SeO 4 ) was added to 100 L. After the addition to the Erlenmeyer flask at a concentration of mg / L, the magnetic bar was put and stirred for 10 minutes in a stirrer at 100 rpm. A 100 mg / L solution of sodium selenate anhydride prepared in spirulina, which was incubated for 11 days, was injected at a 0.35 mL / min aliquot for 48 hours using a peristaltic pump, and then selenium was accumulated in spirulina. After incubation for 48 hours, spirulina was collected and dried. The dried spirulina was then analyzed for selenium content in spirulina using ICP-MS.
As a result, as shown in Table 3, the control group not treated with sodium selenate anhydride was analyzed to have a selenium content of 0.13 mg / kg, and an experimental group to which sodium selenate anhydrous (Na 2 SeO 4 ) was added. A very increased amount of silver selenium content of about 271 mg / kg was detected.
In addition, the production yield analysis, as shown in Figure 2, showed that about 30% yield was improved compared to the control. Therefore, the present inventors have found that when spirulina is cultured by adding sodium selenate anhydride, there is also an effect of increasing the yield by increasing the growth of spirulina.
<Example 2>
Antioxidant Activity of Selenium-Enhanced Spirulina
<2-1> Total Polyphenol Content Analysis
The present inventors analyzed the antioxidant activity of the selenium-enhanced spirulina obtained in Example 1, for this purpose, to inject 10 ml of sodium selenate anhydride per minute so that the final concentration in the medium is 100 mg / L on the 7th day of spirulina culture After 48 hours, spirulina was concentrated and freeze-dried. Then, 2 g of lyophilized sample was added to 40 ml of alcohol ethanol, and refrigerated for 24 hours in a dark condition. Then, 1 ml of extracted sample, 5 ml of folin ciocalteu reagent, and 4 ml of sodium carbonate solution were added to a 15 ml conical tube. After time the total polyphenol content was measured at 765 nm. At this time, a control group without sodium selenate anhydride was used.
As a result, as shown in FIG. 3, the total polyphenol content was about 12% higher in the control group without addition of sodium selenate anhydride.
<2-2> DPPH radical scavenging activity assay
In addition, the present inventors conducted an experiment for analyzing the radical scavenging activity of spirulina with increased selenium.To this end, 10 ml of sodium selenate anhydride was injected per minute so that the final concentration in the medium was 100 mg / L on the 7th day of spirulina culture. After time the spirulina was concentrated and lyophilized. Then, 2 g of lyophilized sample was added to 40 ml of ethanol and refrigerated for 24 hours under dark conditions. In a 15 ml conical tube, 0.8 ml of sample, 4 ml of DPPH, and 3.2 ml of ethanol were added for 30 minutes, followed by 517 nm using a Spectrophotometer. DPPH radical scavenging activity was evaluated by measuring at.
As a result, as shown in Figure 4, the DPPH radical scavenging ability was about 2.6 times higher than the control group without the spirulina group added with sodium selenate anhydride.
Therefore, the present inventors can produce selenium-enhanced spirulina when culturing spirulina by the method according to the present invention, and the selenium-enhanced spirulina shows very excellent antioxidant activity in various industrial fields such as functional health foods and pharmaceuticals. It can be seen that it can be used as a raw material of.
So far I looked at the center of the preferred embodiment for the present invention. Those skilled in the art will appreciate that the present invention can be implemented in a modified form without departing from the essential features of the present invention. Therefore, the disclosed embodiments should be considered in descriptive sense only and not for purposes of limitation. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope will be construed as being included in the present invention.
Claims (9)
The sodium selenate anhydride is injected into the medium for 48 hours at a dose of 0.35ml / min using a peristaltic pump 100mg / L concentration, and then incubated for 48 hours to accumulate selenium in spirulina doing,
Spirulina production method with increased selenium content by increasing the growth of spirulina.
The medium is a medium containing the components of the following table.
The culturing is characterized in that the culture in a condition of temperature of 28 ~ 32 ℃, light quantity 150 μmol / ㎡ / s and photoperiod day (Light): night (Dark) 16: 8.
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