KR102025611B1 - A composition comprising a culture of Ceriporia lamaritus for preventing or treating of atopic dermatitis - Google Patents

Abstract

The present invention relates to a composition for preventing or treating atopic dermatitis, comprising a culture of Ceriporia lamaritus as an effective component. According to the present invention, it was found that the culture of Ceriporia lamaritus contains components effective in alleviating skin inflammations and atopic dermatitis, complex polysaccharides and beta-glucan; newly generates 5-hydroxy-6,7-dimethoxy phthalate; contains a large quantity of effective components, such as inorganic components, amino acids, and vitamins; and is effective in reducing skin moisture loss and alleviating atopic dermatitis. Thus, the culture of Ceriporia lamaritus has a promising potential to be used in the development of therapeutic agents for atopic dermatitis and cosmetics for alleviating atopic dermatitis.

Description

A composition comprising a culture of Ceriporia lamaritus for preventing or treating of atopic dermatitis}

The present invention relates to a composition for preventing or treating atopic dermatitis comprising a culture medium of Ceriporia lamaritus strains as an active ingredient.

Mushrooms are rich in carbohydrates, proteins, lipids, minerals, vitamins, etc., and have been used as food and medicinal materials for a long time because they contain a large amount of bioactive substances including beta-glucan. Many of the biologically active mechanisms have been scientifically proven, and research on the development of artificial cultivation methods for mushrooms, development of mass propagation methods, discovery of new mushrooms, and functional exploration is needed.

Mushrooms can be classified into mycellium and fruit body. Mushroom mycelium contains a number of useful materials as well as fruiting bodies, in particular unlike mushroom fruiting body has the advantage that it is possible to stably short-term culture and production in the container.

Recently, most mushroom spawns have been able to cultivate mycelia containing various bioactive substances through bioconversion using nutrients contained in liquid medium. Since mushroom mycelium has different culture characteristics depending on the medium composition, culture conditions, or mushroom mycelium, appropriate culture conditions must be determined for each mushroom in order to increase the liquid culture efficiency of the mycelium. Various metabolites with anticancer and anti-oxidant activities can be obtained depending on the composition and the culture conditions (Lee Wi-young, et al., 2008).

Ceriporia lamaritus ( Ceriporia lamaritus ) is a novel strain belonging to the Polypoaceae poria (Accession No. KACC 83018BP). Obtained by cross breeding by di-mono hybridization. Mycelia of Seriphoria lamaritus are low-temperature strains resistant to contamination of various bacteria because they are thick and have a high differentiation rate, but they are genetically classified as punctate. It was confirmed that the shape and also different, and has been registered for a patent for a composition for treating diabetes comprising a new strain and its culture (Korean Patent No. 1925890; Korean Patent No. 1969433).

Accordingly, the present inventors report that, in the course of continuous study of Seriphoria lamaritus and its culture, in addition to the complex polysaccharide, beta-glucan, which is effective for skin improvement in Seriphoria lamaritus culture, it is effective in treating atopic dermatitis. It was confirmed that the 5-hydroxy-6,7-dimethoxyphthalide component is newly generated and contains a lot of active ingredients such as inorganic components, amino acids, vitamins, etc., and these cultures improve skin moisture loss and The present invention was completed by confirming that the atopic dermatitis therapeutic effect is excellent.

Conventional prior art, Korean Patent No. 1705482 and Korean Patent Publication No. 2017-0114574 discloses a composition for improving atopic dermatitis containing a culture medium of Seriphoria laccerata mycelium, but the Seriphoria lamari of the present invention There is a difference between Tooth and its composition, and there is no increase in atopic dermatitis improvement or treatment effect of 5-hydroxy-6,7-dimethoxyphthalide and a culture containing the same produced by Seriphoria lamaritus. It is not described and implied. In addition, Korean Patent No. 1522415 describes the skin irritation and moisturizing effect of the composition containing the extract of Seriphoria racerata, but the atopy treatment effect is not described at all, and the Seriphoria lamari of the present invention. Tooth cultures differ in composition.

Korean Registered Patent No. 1925890, A novel composition for preventing or treating diabetes, comprising a new Seriphoria racerata-K1 strain and its culture as an active ingredient, 2018. 11. 30. Registered. Korean Registered Patent No. 1969433, A composition for preventing or treating diabetes, comprising a two-stage culture using the Janna Bulbulocho and Seriporia racerata K1 mycelium as an active ingredient, 2019. 04. 10. Registration. Registered Korean Patent No. 1705482, atopic dermatological lotion or cream containing Ceriporia laccerata, registered on Feb. 03, 2017. Korean Laid-Open Patent No. 2017-0114574, Atopic dermatitis-improving soap containing Ceriporia laccerata, published Oct. 16, 2017. Korean Registered Patent No. 1522415, Cosmetic composition comprising the extract of Seriporia laccerata and cosmetics using the same, 2015. 05. 15. Registered. Registered Korean Patent No. 1736916, Atopic dermatitis improving composition using a phthalide derivative, 2017. 05. 11. Registration. Korean Registered Patent No. 0883342, A pharmaceutical composition for the treatment of atopic dermatitis containing glutamine. 2009. 02. 05. Registration.

Lee, Wi-Young, et al., Liquid Culture of Mushroom Mycelium and Production of Useful Substances, National Forest Research Institute, Report 08-07, National Forest Research Institute, 2008. Yu, W. G., et al., Hepatoprotective Effects of 2 ′, 4′-Dihydroxy-6′-methoxy-3 ′, 5′-dimethylchalcone on CCl4-Induced Acute Liver Injury in Mice, J. Agric. Food Chem., 59, 12821-12829, 2011.

An object of the present invention is to provide a composition for the prevention or treatment of atopic dermatitis, comprising a culture medium of Ceriporia lamaritus strain ( Ceriporia lamaritus ).

The present invention relates to a pharmaceutical composition or cosmetic composition for improvement or prevention of atopic dermatitis comprising a culture medium of Ceriporia lamaritus ( Accession No. KACC 83018BP) as an active ingredient.

The culture culture of Seriphoria lamaritus is 5-hydroxy-6,7-dimethoxyphthalide (5-hydroxy-6,7-dimethoxyphthalide), compared to the culture culture of Seriphoria racerata strain, the parent strain At least one selected from the group consisting of glutamine and aluminum may be newly included.

The culture culture of Seriphoria lamaritus strain may be increased by at least four times the content of at least one selected from the group consisting of vitamins, magnesium and calcium as compared to the culture culture of Seriphoria racerata strain.

The culture culture of the three Seriporia lamaritus may exhibit an effect of reducing the transdermal moisture loss of the skin or increase the skin moisture.

The pharmaceutical composition may be an external preparation for skin.

The present invention also relates to a cosmetic comprising the cosmetic composition.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for the prevention or treatment of atopic dermatitis comprising a culture medium of Ceriporia lamaritus ( Accession No. KACC 83018BP) as an active ingredient.

The Seriphoria lamaritus strain is obtained by the present breeder breeding method by using the existing Seriphoria racerata strain as a parent strain, a new deposited with the accession number KACC 83018BP to the National Academy of Agricultural Science Strain (Korean Patent No. 1925890).

The Ceriporia lacerata was first discovered in Japan in 2003, and is a kind of white fungus that is a by-product of oak or red pine, and can degrade cellulose, hemicellulose, and lignin of wood. .

The seriphoria racerata strain used as the parent strain is the present inventors, in addition to the CL-KU collected at Sogwang-ri, Geumgangsong-myeon, Geumgang-myeon, Uljin-gun, Gyeongbuk, and CL-KS collected at Sogok-ri, Hadong-myeon, Sangju-si, Gyeongbuk, Gwacheon, Jeonbuk, Gunsan, Canada or Japan. It may be a mountain seriporia racerata.

The growth rate of the mycelium spp. Was increased by more than 30% compared to the serifaria racerata strain (CL-KU) collected from Sogwang-ri, Kumgangsong-myeon, Uljin-gun, Uljin, Korea. Is more than twice as slow In addition, it is resistant to the contamination of various bacteria when culturing strains, it is possible to industrialize the high economic efficiency by overcoming the low cultivation success rate due to the contamination by the various bacteria appearing in conventional culture of Seriphoria racerata strain (Korea Patent No. 1925890) ).

The Seriphoria lamaritus strains include 5-dihydroergosterol, DMC (2 ′, 4′-dihyroxy-6′-methoxy-3 ′, 5 ′, as well as complex polysaccharides and beta-glucans. -dimethylchalcone), 5-hydroxy-6,7-dimethoxyphthalide, glutamine, vitamins, aluminum, magnesium, calcium, and the like.

The 5-dihydroergosterol, 5-hydroxy-6,7-dimethoxyphthalide, glutamine and aluminum produced by the Seriphoria lamaritus strain are the parent strains of the Seriphoria racerata strain (the CL Five kinds other than -KU, preferably CL-KU, is a component that is not produced, and the DMC, vitamins, magnesium, or calcium are three strains of the three Seriphoria racerata strains (other than the CL-KU, preferably Is more than four times more Seriporia lamaritus strains than CL-KU).

The DMC is known to have neuroprotective, antidiabetic, spasmolytic, anticancer and anti-inflammatory effects as a major component of buds of Cleistocalyx operculatus (Syzygium nervosum ) (Yu, WG, et al. , 2011).

The 5-hydroxy-6,7-dimethoxyphthalide is included in the flower mushroom, it is known that the effect of treating atopic dermatitis (Korean Patent No. 1736916).

The glutamine is a kind of amino acid, it has been reported to attenuate the inflammatory response to treat atopic dermatitis (Korean Patent No. 0883342).

The aluminum, magnesium and calcium are known to have the effect of strengthening the skin barrier with inorganic elements, promoting skin regeneration, cosmetically removing wastes such as sebum and acne and removing blemishes.

The vitamin is known to have a variety of effects, such as antioxidant, anti-inflammatory, skin improvement, preferably vitamins B, C, D and E.

The Seriphoria lamaritus strain culture may be a mycelium culture derived from the Seriphoria lamaritus strain, and the mycelium culture may be obtained by liquid culture.

The culture may be a culture solution containing both a mycelium and a culture medium, or the mycelium and the culture medium may be separated from the culture medium, respectively. Preferably it is a culture medium separated from the culture solution.

The culture is extracted by adding a liquid culture medium, culture medium powdered culture medium by adding one or more solvents selected from the group consisting of water, C1 ~ C4 lower alcohol, acetone, n-hexane, dichloromethane and ethyl acetate The extract may be, but is not limited thereto.

The culture solution powder can be powdered by using a common drying method of the liquid culture culture.

The lower alcohol of C1 ~ C4 may be methanol, ethanol, propanol, isopropanol, buntanol and the like.

Extraction method of the extract may be selected from any one of hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, compression method. In addition, the desired extract may further be subjected to a conventional fractionation process, it may be purified using conventional purification methods.

Components of the liquid medium in the liquid culture may affect the growth of the strain and the production of the active ingredient. Therefore, it is necessary to establish the components and content conditions of the liquid medium optimized for culturing the Ceriporia lamaritus strain of the present invention.

The liquid medium may include one or more selected from the group consisting of sucrose, glucose, lactose, fructose and galactose as a carbon source, but is not limited thereto. Do not. Preferably sucrose and glucose, more preferably 0.5-3% (w / v) sucrose and 1-5% (w / v) glucose based on the liquid medium, even more preferably Comprises 0.5-2% (w / v) sucrose and 1-3% (w / v) glucose based on liquid medium, and most preferably 1% (w / v) sucrose based on liquid medium And 2% glucose (w / v).

The liquid medium is a nitrogen source of tryptone, yeast extract, soy flour, L-glutamic acid, peptone, malt extract and ammonium ( ammonium) may include one or more selected from the group consisting of, but is not limited thereto. Preferably soybean powder and peptone are included, more preferably soybean powder 1-4% (w / v) and peptone 0.3-1% (w / v) based on the liquid medium. Most preferably it comprises 1.5% (w / v) soybean powder and 0.3% (w / v) peptone based on the liquid medium.

The liquid medium may include one or more selected from the group consisting of KH ₂ PO ₄ , ZnSO ₄ , MgSO ₄ , CuSO ₄ , FeSO ₄ and CaCl ₂ as trace elements, but is not limited thereto. Preferably FeSO ₄ , KH ₂ PO ₄ , MgSO ₄ and CaCl ₂ , more preferably 0.01-0.5% (w / v) FeSO ₄ , 0.1-0.3% (w / v) based on the liquid medium KH ₂ PO ₄ , 0.1-0.3% (w / v) MgSO ₄ and 0.1-0.3% (w / v) CaCl ₂ . Most preferably 0.02% (w / v) FeSO ₄ , 0.15% (w / v) KH ₂ PO ₄ , 0.15% (w / v) MgSO ₄ and 0.15% (w / v) CaCl ₂ based on the liquid medium It includes.

The liquid medium may comprise vitamins. Preferably vitamins B, C and E, but is not limited thereto. More preferably 0.1 to 0.5% (w / v) of each vitamin based on the liquid medium. Most preferably 0.2% (w / v) based on the liquid medium.

The liquid medium may have a pH of 5-7. Preferably pH 6. pH can affect the shape and activity of proteins by changing the charge of the amine group or carboxyl group of amino acids, which are units of enzyme proteins that are important for cellular metabolism. In addition, the change in pH in the external environment may affect the ionization of microbial nutrients, which may affect the microbial intake of nutrients. Therefore, when the pH of the liquid medium is less than 5, it is easy to breed aerobic algae or archae, and when the pH exceeds 7, alkaline actinomyces and mold fungi breed. It is not easy because it is not easy to grow mycelia.

The liquid medium is 1% (w / v) sucrose, 2% (w / v) glucose, 1.5% (w / v) soybean powder, 0.3% (w / v) peptone, FeSO ₄ 0.02 % (w / v), KH ₂ PO ₄ 0.15% (w / v), MgSO ₄ 0.15% (w / v), CaCl ₂ 0.15% (w / v), Vitamin B 0.2% (w / v), Vitamin C 0.2% (w / v) and Vitamin E 0.2% (w / v), with a pH of 6.0 being most preferred.

The culture may use a stirred bioreactor, the growth of the strain may be affected according to the air supply amount, stirring speed, dissolved oxygen amount, incubation temperature and incubation time, the optimum of the Seriporia lamaritus strain of the present invention It is important to establish culture conditions.

The air supply may be 0.02 ~ 1vvm, preferably 0.1 ~ 0.5vvm, more preferably 0.2vvm.

The stirring speed may be 25 ~ 125rpm, preferably 50 ~ 125rpm, more preferably 85rpm.

The dissolved oxygen amount may be 5 to 40%, preferably 10 to 40%, and more preferably 20%.

The culture temperature may be 20 ~ 25 ℃, preferably 22 ℃. If the temperature is less than 20 ℃, the activity of the mycelium is slowed down, the incubation period is long, the productivity is lowered, the production cost is rapidly increased. If the temperature is above 25 ℃, the mycelium is enlarged, the mycelial amount increases rapidly, but the production of the active ingredient is extremely high. It is lowered, which is not preferable.

The incubation time may be 6 to 15, preferably 7 to 10 days, more preferably 10 days. If the incubation time is less than 6 days, the content of the active ingredient is low, if it is more than 10 days, the content of the active ingredient is no longer increased or gradually decreased, and if more than 15 days, the content of the active ingredient is sharply reduced. Not desirable

The culture culture of the three strains of Seriphoria lamaritus may have a sugar content of 0.5 to 1 brix, a viscosity of 2 to 10 Cp, and a water content of 95 to 98% in the supernatant obtained by centrifuging the culture solution.

The culture culture of the S. liporia Ramaritus strain is concentrated or extracted with a sugar content of 45 ~ 55brix, viscosity 4,500 ~ 5,500Cp, water content 10 ~ 15% to maintain the functionality of the culture and not impair the physical properties of the pharmaceutical or cosmetic composition Can be used. Preferably, the sugar content is concentrated or extracted at 48-52 brix, viscosity 4,800-5,200 Cp, water content 12-13%.

The culture culture of S. aureus not only contains complex polysaccharide and beta-glucan, but also 5-hydroxy-6,7- which is not included in the cultivar of S. aureus Serrata strain. One or more selected from the group consisting of dimethoxyphthalide, glutamine and aluminum may be newly included. In addition, compared to the culture culture of the mother strain of Seriporia racerata strain may contain 5.5 times more vitamins, 8 times magnesium, 4.2 times calcium.

The culture culture of the three Seriporia lamaritus can prevent skin moisture loss by reducing the transdermal moisture loss of the skin or increase the skin moisture.

The culture culture of Seriporia lamaritus may have an atopic dermatitis improvement, prevention or treatment effect.

In addition, the present invention relates to a pharmaceutical composition for preventing or treating atopic dermatitis, comprising a culture of Seriporia lamaritus strain.

The pharmaceutical composition may comprise the Ceriporia lamaritus strain culture and a pharmaceutically acceptable excipient.

The Seriphoria lamaritus strain culture is preferably added in an amount of 0.001 to 50% by weight, more preferably 0.001 to 40% by weight, and most preferably 0.001 to 30% by weight based on the total weight of the pharmaceutical composition. Can be.

The pharmaceutical compositions may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, suppositories, and sterile injectable solutions according to conventional methods. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations include at least one excipient such as starch, It is prepared by mixing calcium carbonate, sucrose or lactose, gelatin and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween-61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The pharmaceutical composition is preferably a topical skin formulation. The external preparation for the skin may be a spray, gel, ointment, cream, etc., but is not limited thereto.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. Dosage determination based on these factors is within the level of those skilled in the art and generally dosages range from about 0.1 mg / day to about 2000 mg / day. More preferred dosage is 0.1 mg / day to 1000 mg / day. Administration may be once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The pharmaceutical composition of the present invention can be administered to mammals such as rats, livestock, pets, humans and the like by various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, abdominal, muscle, subcutaneous, skin, intrauterine dural or cerebrovascular injections.

The pharmaceutical composition may further include a known compound or plant extract known to have an atopic dermatitis improving effect.

The present invention also relates to a cosmetic composition for improving atopic dermatitis comprising the culture culture of the three Seriporia lamaritus.

The cosmetic composition is an auxiliary agent commonly used in the culture of Seriphoria lamaritus strains and cosmetics, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blockers , Pigments, odorants or dyes.

The culture culture of the three Seriporia lamaritus may be contained 0.001 to 50% by weight, preferably 0.001 to 30% by weight based on the total weight of the cosmetic composition.

The amount of the adjuvant is an amount conventionally used in the art, such as 0.001 to 50% by weight, preferably 0.001 to 70% by weight based on the total weight of the cosmetic composition. However, in any case, the adjuvant and the ratio thereof may be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

The cosmetic composition may further comprise a known compound or plant extract known to have an effect of improving atopic dermatitis in addition to the culture culture of Seriporia lamaritus.

The cosmetic composition may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils , Powder foundations, emulsion foundations, wax foundations, sprays, and the like. Preferably, the flexible cosmetics, nourishing cosmetics, massage cream, nourishing cream, pack, gel, can be prepared in the formulation of the skin adhesive type, but is not limited thereto.

The present invention provides a cosmetic comprising the cosmetic composition.

The cosmetic may be composed of one or more formulations selected from the group consisting of lotion, skin softener, skin toner, astringent, cream, foundation, essence, pack, soap, cleansing foam and body cleanser, but is not limited thereto.

The present invention provides a health functional food composition for improving atopic dermatitis comprising the culture culture of the three Seriporia lamaritus.

The dietary supplement composition may comprise a culture culture Seriporia lamaritus strain and food supplements additives.

The dietary supplement composition of the present invention includes the form of tablets, capsules, pills or liquids, and the food to which the culture of the present invention may be added, for example, various foods, beverages, gums, teas, Vitamin complexes and health functional foods.

The present invention relates to a composition for improving or treating atopic dermatitis comprising a culture medium of Ceriporia lamaritus ( Ceriporia lamaritus ) strain as an active ingredient, and a complex known to improve atopic dermatitis in a culture medium of Seriporia lamaritus . In addition to polysaccharides and beta-glucans, 5-hydroxy-6,7-dimethoxyphthalide was newly generated, and it was confirmed that it contained many active ingredients such as inorganic components, amino acids, and vitamins. It was confirmed that the skin moisture loss improvement and atopic dermatitis treatment effect is excellent.

Through this, it is expected that the Ceriporia lamaritus culture of the present invention can be usefully used for developing atopic dermatitis therapeutics or cosmetics for improvement.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the information provided herein is to be thorough and complete, and to fully convey the spirit of the present invention to those skilled in the art.

Example 1 Preparation of Ceriporia Lamaritus Culture

Novel Ceriporia Lamaritus of the Invention lamaritus ) is the present inventors CL-KU collected from Sogwang-ri, Geumgang-myeon, Uljin-gun, Gyeongbuk, and CL-KS collected from Sogok-ri, Hadong-myeon, Sangju-si, Gyeongsangbuk-do, Korea. As a new strain breeding and breeding by dinuclear mononuclear (di-mono) breeding method using a parent strain strains (accession number KACC 83018BP) and patent registration (Korean Patent No. 1925890).

To cultivate Seriaporia lamaritus strains, 1% (w / v) sucrose, 2% (w / v) glucose, 1.5% (w / v) soybean powder, 0.3% peptone, based on 4,000 liters of culture. w / v), FeSO ₄ 0.02% (w / v), KH ₂ PO ₄ 0.15% (w / v), MgSO ₄ 0.15% (w / v), CaCl ₂ 0.15% (w / v), and vitamin B , C, E is dissolved into the purified water to each improved to be 0.2% (w / v), after that the pH of the slurry reached 6.0, 121 ℃, pressure 1.5㎏f / ㎝ ² cooling conditions in the sterilization and 22 ℃ 20 bungan Culture medium was prepared.

The culture medium prepared above was placed in a 5,000-l bioincubator, inoculated with 30 l of the strain for inoculation of Seriaporia lamaritus, with an air supply of 0.2vvm, agitation speed of 85rpm, dissolved oxygen of 20%, culture temperature of 22 ° C, pH 6.0 Incubated for 10 days under conditions. Subsequently, the culture solution was centrifuged to separate the supernatant, and 3000 L of the separated supernatant was concentrated using a reduced pressure concentrator until it became 100 L at 55 ° C., thereby preparing a culture culture of Saliforia lamaritus.

As a result of confirming the characteristics of the culture medium of the above-mentioned Seriphoria lamaritus, it was confirmed that the sugar content of 50Brix, viscosity 5,000Cp, water content of 12.5%.

Example 2. Component Analysis of Ceriforia Lamaritus Culture

In order to confirm the active ingredients and their contents contained in the culture medium of Seriphoria lamaritus prepared in Example 1, the analysis was requested to the local food research institute. Analysis items are mushroom extracts, polysaccharides, beta-glucan, 5-hydroxy-6,7-dimethoxy phthalide, which are known to be effective in improving skin inflammation and treating atopic dermatitis. ), Glutamine, vitamins, aluminum, magnesium, calcium were analyzed, and the results are shown in Table 1 below. At this time, the present inventors used a culture medium of the seriporia racerata strain (CL-KU), the origin of Sogwang-ri, Kumgangsong-myeon, Uljin-gun, Uljin-gun, among the strains used as a parent strain when breeding the seriporia lamaritus strain.

Analysis item Culture Ceriforia La Serrata Ceriforia Lamaritus Complex polysaccharide 41.11 (% / ml) 45.26 (% / mL) Beta-glucan 39.32 (% / ml) 38.04 (% / mL) 5-hydroxy-6,7-dimethoxyphthalide - 18.51 (μg / ml) Glutamine - 1.20 (% / ml) vitamin 1.01 (% / mL) 5.51 (% / mL) aluminum - 0.61 (% / mL) magnesium 0.44 (% / mL) 3.52 (% / mL) calcium 0.65 (% / mL) 2.74 (% / ml)

As shown in Table 1, the content of the complex polysaccharide and beta-glucan is similar to that of the culture medium of Ceriporia lamaritus and Ceriporia racerata, while 5-hydroxy- is known to improve atopic dermatitis. It was confirmed that 6,7-dimethoxyphthalide was contained only in Ceriporia lamaritus culture. In addition, it was confirmed that the inorganic components such as vitamins, magnesium, and calcium, glutamine and vitamins were significantly contained in the Ceriporia lamaritus culture as compared to the Ceriporia racerata culture.

Through this, it was found that the Ceriporia lamaritus culture of the present invention contains a large amount of various effective ingredients effective for improving or treating atopic dermatitis.

<Example 3. Confirmation of the improvement effect of skin moisture loss of the culture of Seriphoria lamaritus>

In general, atopic dermatitis is characterized by a loss of moisture due to the collapse of the skin barrier and poor moisturization. Accordingly, the effect of treating atopic dermatitis by analyzing the degree of water loss of the skin by measuring trans epidermal water loss (TEWL) and stratum corneum hydration (SCH) of the culture of the Seriporia lamaritus culture of the present invention It was confirmed.

The test was conducted on five female subjects of neutral skin type. Purchasing a body lotion of a commercially available A company in Korea, and after mixing each of the culture lotion culture medium prepared in Example 1 or 5 ml each of the Ceriporia las cerata culture prepared in Example 1 per 100 ml The subjects were given 20 ml each, and 20 ml of body lotion was added. The method was used twice a day, after washing hands in the morning and evening, 0.5ml each time applied to the back of the hand and forearm. Apply nothing to the right forearm, the body lotion to the left forearm, the body lotion containing the Seriphoria racerata culture on the back of the right hand, and the body lotion containing the culture of Seriphoria lamaritus on the back of the left hand. Applied.

In order to confirm the effect of improving skin moisture loss, each of the body lotion was applied for 5 days, and the body barrier measuring device was applied without a skin lotion on the 6th day, a gpskin barrier (gppower barrier, Korea) Transdermal moisture loss (TEWL) and skin moisture content (SCH) were measured using the results, and the results are shown in Table 2 below.

Subject No coating Body Lotion No addition Add Ceriporia racerata culture Add Ceriaporia lamaritus culture TEWL (g / ㎡ / h) SCH (%) TEWL (g / ㎡ / h) SCH (%) TEWL (g / ㎡ / h) SCH (%) TEWL (g / ㎡ / h) SCH (%) Subject-1 15 21 11 30 6 56 4 62 Subject-2 33 17 30 25 12 49 9 55 Subject-3 39 16 32 22 15 42 12 48 Subject-4 25 19 21 24 10 51 6 57 Subject-5 44 13 37 19 20 36 15 41 Average 31.2 17.2 26.2 24 12.6 46.8 9.2 52.6

As shown in Table 2, compared to the case of applying a body lotion or a body lotion containing a Ceriporia racerata culture in all subjects, a body lotion containing a Ceriporia lamaritus culture was applied. In this case, the amount of transdermal moisture loss was low, and the skin moisture content was maintained to be high.

Through this, the Ceriporia lamaritus culture of the present invention has a remarkably superior skin moisture loss improvement effect compared to the Ceriporia racerata culture known to have a moisturizing effect, thereby improving or treating atopic dermatitis. I could see that.

<Example 4. Confirmation of atopic dermatitis improvement effect of Ceriporia lamaritus culture>

NC / Nga mice, which are atopic dermatitis models, were used to confirm the atopic dermatitis treatment or amelioration effect of the culture of the present invention.

Five NC female N / Nga mice purchased from the central experimental animals were randomly assigned to three rats per treatment group. Mice were stabilized for 5 days after hair removal using hair removal cream. Thereafter, the treated group treated with nothing was treated as a normal group, and 200 µl of 1% DNCB (dinitrochlorobenzene) dissolved in a solvent mixed with acetone and olive oil in a volume ratio of 3: 1 for two weeks, three times a week. Application resulted in atopic dermatitis. After induction of atopic dermatitis, Ceriporia racerata culture 10 mg / kg (Experimental Group-1) or Ceriporia lamaritus culture prepared in Example 1 5 mg / kg ( Experimental Group-2) or 10 mg / kg (Experimental Group-3) were applied respectively. The positive control group applied tacrolimus (10 mg / kg) used as atopic dermatitis treatment, and the negative control group applied nothing after induction of atopic dermatitis. After treatment, blood was collected from the heart of the sacrificed mice to separate serum, and serum IgE was measured according to the manufacturer's manual using an IgE ELISA kit (BD Biosceinces, USA). The relative IgE ratio (%) of each treatment group was calculated based on the IgE of the negative control group that induced atopic dermatitis by DNCB treatment, and the results are shown in Table 3 below.

Treatment group Coating sample IgE (%) Normal No treatment 9 Negative Control Apply only DNCB 100 Positive control group DNCB + Tacrolimus 10mg / kg 67 Experimental Group-1 DNCB + Ceriporia racerata culture 10 mg / kg 36 Experiment group-2 DNCB + Ceriporia lamaritus culture 5 mg / kg 32 Experiment group-3 DNCB + Ceriporia lamaritus culture 10 mg / kg 26

As shown in Table 3, the serum IgE of the positive control group coated with tacrolimus, a therapeutic agent for atopic dermatitis, was reduced to 67% compared to the negative control group coated with DNCB only. In addition, serum IgE of Experimental Group-1 to Experiment-3, which was coated with either Ceriporia racerata culture or Ceriporia lamaritus culture, was reduced to 36%, 32% and 26%, respectively, which was negative control. It was confirmed that the value is more than 2.5 times lower than. In particular, the serum IgE of Test Group-3, which was coated with Ceriporia lamaritus culture 10 mg / kg, was 26%, compared to 36% of Test Group-1, which was applied with the same amount of Ceriporia Lacetara culture. I found that my IgE decreased more.

Through this, it can be seen that the seriporia lamaritus culture of the present invention has a remarkably superior atopic dermatitis treatment or improvement effect than the seriporia laseriata culture, It can be predicted that the effect of the included 5-hydroxy-6,7-dimethoxyphthalide to improve atopic dermatitis.

Claims (10)

Ceriporia lamaritus (Accession No. KACC 83018BP) culture containing 5-hydroxy-6,7-dimethoxyphthalide as an active ingredient Atopic dermatitis prevention or treatment comprising a pharmaceutical composition. The method of claim 1,
Said Seriphoria lamaritus strain culture is prevented or treated for atopic dermatitis, characterized in that it comprises one or more selected from the group consisting of glutamine and aluminum compared to the Seriphoria racerata strain culture as a parent strain Pharmaceutical composition for. The method of claim 1,
The culture culture of Seriphoria lamaritus is characterized in that the content of at least one species selected from the group consisting of vitamins, magnesium and calcium compared to the culture culture of Seriphoria racerata strains of the parent strain four times or more. Pharmaceutical composition for preventing or treating atopic dermatitis. The method according to any one of claims 1 to 3,
Said Seriphoria lamaritus strain culture is a pharmaceutical composition for preventing or treating atopic dermatitis, characterized in that the effect of reducing the transdermal moisture loss of the skin or increase the skin moisture. The method according to any one of claims 1 to 3,
The pharmaceutical composition is a pharmaceutical composition for preventing or treating atopic dermatitis, characterized in that the external preparation for the skin. The 5-hydroxy-6,7-methoxy shifted three reports Ria La Marie Tooth strain containing deionized fluoride (5-hydroxy-6,7-dimethoxyphthalide ) (Ceriporia lamaritus, accession No. KACC 83018BP) culture as an active ingredient Cosmetic composition for improving atopic dermatitis comprising. The method of claim 6,
Said Seriphoria lamaritus strain culture is a cosmetics for improving atopic dermatitis, characterized in that it comprises a new strain at least one selected from the group consisting of glutamine and aluminum compared to the culture strain of Seriphoria racerata strain Composition. The method of claim 6,
The culture culture of Seriphoria lamaritus is characterized in that the content of at least one species selected from the group consisting of vitamins, magnesium and calcium compared to the culture culture of Seriphoria racerata strains of the parent strain four times or more. Cosmetic composition for improving atopic dermatitis. The method according to any one of claims 6 to 8,
Said Seriphoria lamaritus strain culture is a cosmetic composition for improving atopic dermatitis, characterized in that the effect of reducing the transdermal moisture loss of the skin or increase the skin moisture. Cosmetics containing the cosmetic composition of any one of Claims 6-8.