KR102016077B1 - Food compositions for improving and preventing atopic dematitis skin condition - Google Patents

Abstract

The present invention relates to a food composition for preventing and ameliorating atopic dermatitis, comprising the extracts of Lonicera japonica, Houttuynia cordata, dried Perilla frutescens, Lonicera japonica, Artemisia annua, Maclura leaves, Maclura branches, dandelion, Prunella vulgaris, Zizania latifolia, Portulaca oleracea, Orostachys japonica, Ixeridium dentatum, root barks of elm tree, Xanthium sibiricum, Plantago asiatica, peppermint, Saururus chinensis and Poncirus trifoliate, as active ingredients.

Description

FOOD COMPOSITIONS FOR IMPROVING AND PREVENTING ATOPIC DEMATITIS SKIN CONDITION}

The present invention relates to a food composition for preventing and improving atopic dermatitis.

Atopic dermatitis is a chronic, recurrent inflammatory skin disease that usually begins in infancy or childhood, accompanied by pruritus (itch), dry skin, and characteristic eczema. In infancy, it begins with eczema on the flanks of the face and limbs, but as it grows, it is characteristic of eczema on the bends of the arms and behind the knees, and in many cases it tends to improve naturally as it grows. . In adults, the skin becomes thicker in the folding area, and more eczema occurs on the face than in infants. Atopic dermatitis is on the rise worldwide, and the prevalence is reported to be 20% of the population.

The cause of the development of atopic dermatitis is not yet known. Because clinical symptoms also appear in various cases such as dry skin and eczema, the cause of the disease cannot be explained by any one, but environmental factors, genetic predisposition, immunological reactions and abnormal skin barriers are considered to be the main causes. Environmental factors include allergens that cause allergies, such as environmental pollution, such as smoke from industrialization, increased use of food additives, increased use of carpets, beds, and sofas due to Western-style dwellings, and house dust mites due to increased room temperature. Allergens). In addition, as pets grow more indoors, exposure to causative agents is also a cause. On the other hand, the genetic effect of atopic dermatitis can be seen in the fact that many patients with atopic dermatitis have a family history.

Atopic dermatitis often improves or disappears with age, but even when it improves, it tends to easily itch or inflammatory by specific substances or stimuli, and hand eczema often appears in adulthood. If atopic dermatitis remains until adulthood, the skin symptoms of the body improve, while erythema, redness, and eczema tend to appear on the face, and the folding area has a long rubbing skin, which shows the appearance of thickened skin. In adulthood, not only chronic eczema is present, but acute lesions with scabs and scabs on chronic eczema occur repeatedly over a period of time.

Known treatments for such atopic dermatitis include washing. Cleaning is to take a shower or bath to keep the skin clean, and it is important to remove the deterioration factors of atopic skin by washing away dust, house dust mites, harmful bacteria, sweat, and other foreign substances on the skin. In particular, if you sweat a lot, it is necessary to wash well, and if not properly cleaned, or excessive cleaning may worsen the skin condition.

In addition, there is a method of applying ointment by applying directly to atopic dermatitis, but common side effects for steroids include skin atrophy, vasodilation, discoloration, purpura, weakening the skin and capillary due to weakened skin Blood vessels may be exposed. The above method may be less effective as the resistance is used, and there may be a rebound phenomenon (recurrence) due to the discontinuation of use, so the method of applying ointments such as steroids should be avoided if possible without severe symptoms. .

Another way is to take internal medicine. This is what the effect is hoped for by taking steroids. As with steroid ointments, this can also have a temporary effect, but continued use can cause the above side effects.

KR 10-1839064 B1 KR 10-1043509 B1

Therefore, the object of the present invention is a gold silver flower, Eoseongcho, Jassop leaf, Indongcho, Firefly, Kudji mulberry leaf, Kudji branch, Dandelion, Hagocho, gwachocho, Machihyeon, Wasong, Seogwi, Yugeunpi, Changja, Chajeoncho, mint, triticale, tanza By using to provide a food composition that can prevent and improve atopic dermatitis without side effects.

Another object of the present invention is to provide a food composition that can improve skin itching.

Another object of the present invention is to provide a food composition having excellent antioxidant power.

Food composition for preventing and improving atopic dermatitis of the present invention for achieving the above object, each of 100g gold and silver, eoseongcho, jasmine leaf, Indongcho, firewood, kkujippong leaves, kkujippongji, dandelion, haegocho, gyochocho, Machihyun, Wasong, nibbark, Yugeunpi, Changjaja, Chajeoncho, peppermint, three hundred seconds, tanza dried products were prepared and pulverized to 100mesh, 20 weight times of water was added, extracted and filtered for 10 hours at 80 ℃ effective It is contained as a component, the extract is extracted with water as a solvent, the extract is Geumhwa hwaseong, Eoseongcho, Jassop leaf, Indongcho, Ganoderma lucidum, Cudrania leaf, Cudrania branch, Dandelion, Hagocho, Korean gyocho, Machihyeon, Wasong, Seogwi , 10-30 weight times of water is added to the mixture of root skin, changja, chajeoncho, mint, 300 seconds and tanza, and can be extracted at 40-100 ° C. for 5 to 24 hours.
The raw material of the extract is 100 parts by weight of gold and silver, 10-200 parts by weight of eoseongcho, 10-200 parts by weight of Japonica leaves, 10-200 parts by weight of Indongcho, 10-200 parts by weight of firewood wormwood, 10-200 parts by weight of Cudrania leaf, Cudrania Eggplant 10-200 parts by weight, dandelion 10-200 parts by weight, hachocho 10-200 parts by weight, high-fat candle 10-200 parts by weight, guchi Prefecture 10-200 parts by weight, Wasong 10-200 parts by weight, moth 10-200 parts by weight It may be 10-200 parts by weight, root skin 10-200 parts by weight, Chang-ja 10-200 parts by weight, 10-200 parts by weight of mint, 10-200 parts by weight of mint, 10-200 parts by weight of 300 seconds, and 10-200 parts by weight of a tanza.

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According to the present invention, it is possible to prevent and improve atopic dermatitis only by ingesting the food composition, and pruritus (itch) may also be improved.

1 is a graph showing the degree of skin damage according to Test Example 5 of the present invention.
Figure 2 is a graph showing the amount of serum IgE expression according to Test Example 5 of the present invention.
Figure 3 is a graph showing the amount of serum histamine expression according to Test Example 5 of the present invention.
Figure 4 is a graph showing the measurement results of the AST according to Test Example 5 of the present invention.
5 is a graph showing the measurement results of the ALT according to Test Example 5 of the present invention.
6 is an optical micrograph of the epidermis stained with Hematoxylin & eosin (H & E) according to Test Example 5 of the present invention.
7 is a graph showing the thickness measurement results of the epidermis according to Test Example 5 of the present invention.
8 is a graph showing the expression level of iNOS according to Test Example 5 of the present invention.
9 is a graph showing the expression amount of COX2 according to Test Example 5 of the present invention.

Hereinafter, the present invention will be described in detail.

Food composition for preventing and improving atopic dermatitis according to the present invention, gold and silver, eoseongcho, Jassop leaf, Indongcho, firewood, Cudrania leaf, Cudrania branch, Dandelion, Hachocho, Jijichocho, machihyeon, wasong, bittersweet, Yugeunpi, Changza, It is characterized by containing the extract of chajeoncho, peppermint, 300 seconds and tanza as an active ingredient. That is, such extracts have excellent antioxidant activity, and by this antioxidant activity, the removal rate of free radicals is very high, thereby preventing and improving atopic dermatitis.

Here, the food composition includes, but is not limited to, a beverage, a tea, as well as any one of powders, granules, pills, tablets, capsules, candy, and syrups.

And the food composition may include the extract in an amount of 1 to 100% by weight as an active ingredient.

In addition, the food composition of the present invention may further contain a known additive. These known additives include various additives added to known foods, including citric acid, sugars, white sugar and the like. In addition, the food composition of the present invention does not limit the intake amount.

For example, when the composition of the present invention is used as a health beverage, various flavors or natural carbohydrates and the like may be contained as additional ingredients, as in the general beverage. The above-mentioned natural carbohydrates are sugars such as monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as textine and cyclotenstrin, xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used.

Extracted raw materials such as gold, silver, Eoseongcho, Jassop leaf, Indongcho, Gadfly, Kudji leaf, Cudang branch, dandelion, haegocho, gwachocho, machihyeon, wasong, casserole, yugeunpi, changja, chajeoncho, peppermint, triticale and tanza It may be in a state, and may be in a dried form, but is not limited thereto.

In the present invention, the extract is extracted with water as a solvent, the gold and silver coins, Eoseongcho, Jassop leaf, Indongcho, Ganoderma lucidum leaf, Cudrania leaf, Cudrania branch, Dandelion, Hachocho, Jijacho, Machihyeon, Wasong, Seogwi, Yugeunpi, Changja, 10 to 30 times by weight of water is added to the mixture of chajeoncho, peppermint, 300 seconds and tanza, extracted and filtered for 5 to 24 hours at 40 ~ 100 ℃. Since such extraction method is well known in the art to which this technique belongs, a detailed description thereof will be omitted. In addition, it is natural that it can be concentrated and dried as needed.

At this time, the extract raw material is 100 parts by weight of gold and silver, 10-200 parts by weight of eoseongcho, 10-200 parts by weight of Jassop leaf, 10-200 parts by weight of Indongcho, 10-200 parts by weight of wormwood leaves, 10-200 parts by weight of Cudrania leaf, Cudrania Eggplant 10-200 parts by weight, dandelion 10-200 parts by weight, hachocho 10-200 parts by weight, high-fat candle 10-200 parts by weight, guchi Prefecture 10-200 parts by weight, Wasong 10-200 parts by weight, moth 10-200 parts by weight , 10 ~ 200 parts by weight, root skin 10 ~ 200 parts by weight, 10 ~ 200 parts by weight of chajeoncho, 10 ~ 200 parts by weight mint, 10 ~ 200 parts by weight of 300 seconds and 10 to 200 parts by weight of tanza is preferred, but must be limited It is not.

A food composition comprising an extract as described above and extracts by adding water to it, has the advantage of preventing and improving atopic dermatitis, as well as itching.

Hereinafter, briefly look at each of the extraction raw materials as follows.

The gold and silver coin means a bud of a honeysuckle or a variant thereof, and its pharmacological action includes antibacterial action, anti-inflammatory action, antipyretic action, leukocyte phagocytosis, central nervous excitability, serum cholesterol lowering action, and ulcer. Prevention, etc.

The eoseongcho refers to the ground portion of the flowering period of the weak wheat of more than three hundred, and the pharmacological action includes antibacterial action, immune enhancing action, anti-inflammatory action, diuretic action, antitussive action and the like.

The foliar means a leaf of perennial herb that is an annual herb with the calliphytic plant Lamiaceae, and the pharmacological action includes antipyretic action, healthy action, antibacterial action, antiseptic action and the like.

The indongcho means stems and branches of the honeysuckle, and the pharmacological action is an antibacterial action, a healthy action and the like.

The wormwood refers to the ground part of the wormwood, wormwood plant, the pharmacological action is antipyretic action, anti-inflammatory action, antimalarial action, anti-blood worms (抗 血吸蟲) action, immunoregulatory action, anti-tumor action, antibacterial action, Antiviral action.

The kkujippong is a deciduous broad-leafed arborescent of the Mulberry family, using its leaves and branches. The leaves of the kkujippong is effective for arthritis, skin diseases, eczema, etc., the branch is effective for neuralgia, hepatitis, uterine disease.

The dandelion refers to a plant before flowering, and is effective in swelling, mastitis, sore throat, appendicitis, peritonitis, acute hepatitis and jaundice.

The edible flower refers to the flower stalk of the edible fruit, and its pharmacological action includes blood pressure lowering action, antiviral action, anti-inflammatory action, analgesic action and hypoglycemic action.

The sakuracho is also called cheoncho, means the root of the sachocho. The pharmacological action of the hypertonia is hemostatic action, antitumor action, antibacterial action, antiviral action, antioxidant action, anti-aging action and the like.

Machihyeon means the outpost of purslane, its pharmacological action is antimicrobial action, uterine smooth muscle contraction due to enhancement of contractile force, diuretic action and the like.

The wasong means the outpost of the rock brush, the pharmacological action, such as antipyretic action, antibacterial action.

The moth means the outpost of the moth, and the pharmacological action is a pharmacological effect, such as antipyretic action, sanitary action, hematopoietic action, swelling action.

The roots of the roots of the root of the root of the elm (Ulmus macrocarpa Hance) of the cork bark, also referred to as milky skin. The roots of the roots have pharmacological effects such as anticancer action.

The changja means the fruit of the Asteraceae of the Asteraceae, and its pharmacological action includes hypoglycemic action, leukocyte reduction prevention action, antitussive action, cardiac suppression action, and the like.

The chajeoncho means the outpost of plantain, the pharmacological action is expectoration (작용) action, subterranean (액) action, gastric juice secretion control action, anti-ulcer action, anti-inflammatory action, anti-tumor action, antibacterial action, etc. .

The peppermint means the ground part of the peppermint, and its pharmacological action includes antipyretic action, anti-inflammatory action, palliative action, bile secretion promoting action and gastrointestinal smooth muscle suppression action, increased mucus secretion of the respiratory tract, central nervous excitatory action, uterine contraction Action.

The three hundred seconds refers to the leaves and stems of three hundred seconds, the pharmacological action is a diuretic action, hypoglycemic action, anti-inflammatory action, antibacterial action, platelet aggregation inhibitory action.

The tanza refers to the fruit of the tanza, its pharmacological action includes detoxification, urticaria, dermatitis and eczema relief, anti-inflammatory action.

Hereinafter, the present invention will be described in detail through examples.

(Example 1)

100g each of gold and silver, Eoseongcho, Jassop leaf, Indongcho, Gadfly, Kudji leaf, Cudrania branch, Dandelion, Hagocho, Ganjacho, Machihyeon, Wasong, Mugwort, Yugeunpi, Changiza, Chajeoncho, Mint, Triticale and tanza It was. The dried product was pulverized to about 100 mesh, 20 weight times of water was added thereto, and the active ingredient was extracted at 80 ° C. for 10 hours. And it was filtered with filter paper (Hyundai, No. 10).

 (Test Example 1)

In order to confirm the hazards of the food composition according to Example 1, a request was made to inspect heavy metals and residual pesticides at the Daegu Haany University Oriental Medical Life Research Institute. And the results are shown in Table 1 below.

As shown in Table 1, the food composition according to Example 1 was confirmed that all of lead, arsenic, mercury, cadmium, Diditi, dieldrin, BC, Aldrin, and endrin were not detected.

Test Example 1 Results Inspection items result heavy metal lead 0 ppm arsenic 0 ppm Mercury 0.0 ppm cadmium 0.0 ppm Residual pesticide Total Diti Not detected Dieldrin Not detected Total BH Not detected Aldrin Not detected Endrin Not detected

(Test Example 2)

In order to confirm the quality of the food composition according to Example 1, a request was made to inspection of tar pigment, lead, bacterial count, and E. coli group to the DAI analysis center. And the results are shown in Table 2 below.

As shown in Table 2, the food composition according to Example 1 was confirmed that all tar, lead, bacteria, coliform group was not detected.

Test Example 2 Results Inspection items Inspection standard result Judgment Tar color Not detected Not detected fitness Lead (mg / kg) 0.3 or less 0.0 fitness Number of bacteria (per 1 ml) 100 or less 0 fitness Coliform group voice voice fitness

(Test Example 3)

In order to confirm the antimicrobial activity of the food composition according to Example 1, an antimicrobial activity test for Pseudomonas aeruginosa, Escherichia coli, enteritis vibrio bacillus, and Staphylococcus aureus was commissioned to the Korea Testing Institute. At this time, the test method was in accordance with KS K 0693: 2011, the results are shown in Table 3 below.

As shown in Table 3, the food composition according to Example 1 was confirmed that the antimicrobial activity against Pseudomonas aeruginosa, E. coli, enteritis Vibrio, Staphylococcus aureus.

Test Example 3 Results division Antibacterial activity (%) pseudomonas aeruginosa
ATCC 27853 99.9 Escherichia coli
ATCC 8739 99.9 Vibrio parahaemolycius
ATCC 17802 99.9 Staphylococcus aureus
ATCC 6538 99.9

(Test Example 4)

The content of β-carotene and total flavonoids in the food composition according to Example 1 was tested by the Food Analysis Research Center of Suwon Women's University. And the results are shown in Table 4 below.

As shown in Table 4, the food composition according to Example 1 was confirmed that the content of β-carotene and total flavonoids is very high. It was confirmed that the content of the flavonoids is about 1.3 times or more than the situation mushroom (control group) having the highest flavonoid content among the medicinal mushrooms.

Test Example 4 Results Test Items Test result β-carotene (μ / 100 g) 6.98 Total Flavonoids (mg / 100g) 4,831.02 Total Flavonoids of Controlled Mushroom (Control) (mg / 100g) 3750

(Test Example 5)

The improvement effect on the atopic dermatitis of the food composition according to Example 1 was tested.

sample

The food composition of Example 1 was lyophilized in a freeze dryer (Eyela, Japan). The yield was 25g / 100mL, and the recovered powder was stored at -20 ° C and diluted to a suitable concentration with purified water when used in the experiment.

Experimental animals

Four-week-old male Balb / c mice (Daehan biolink, Korea) were purchased and used for experiments after purification. The temperature of the feeding room was maintained at 22 ± 2 ℃ and humidity 50 ± 5%. The photoperiod and dark cycle were 12 hours. Animal testing was conducted with the approval of the Animal Experiment Ethics Committee of Daegu Haany University.

Induction and Sample Processing of Atopic Dermatitis

After epilation of the back of the experimental animals, 2,4-dinitrochlorobenzene (DNCB, Sigma, USA) was diluted with 10% acetone and olive oil mixture (3: 1 volume ratio) from the next day and applied to the back for 3 days. Two days after the induction of the first immune response, DNCB diluted to 0.5% was applied every other day to induce atopic dermatitis.

Two weeks later, the sample was diluted four times a day (10:00, 12:00, 14:00, 16:00) in distilled water at 0.01, 0.5, 1 g / mL, and orally administered at 100 uL for 2 weeks. In addition, during the sample administration period, DNCB was continuously applied every other day to suppress natural healing.

In the control group, 100 uL of purified water was orally administered for 2 weeks in place of the sample, and as a positive control group, terfenadine was applied to the atopic dermatitis-induced site.

Test result

end. Skin damage measurement

The clinical skin severity score was measured using clinical visual evaluation. The application of DNCB solution causes experimental animals to develop erythema / pigmentation, edema, exudation / dust, abrasions, thyroiditis, and keratin / skin dryness.This item is absent (0), mild (1), severe (2), severe It divided into (3) and scored. The result was as shown in FIG.

After oral administration of the sample four times a day for two weeks, the skin damage of the test animal was measured. As shown in FIG. 1, the control group coated with DNCB was 8.0 ± 0.24 compared to the normal group coated with only purified water as a solvent. The skin was significantly damaged and the experimental group, which was the experimental group, was 6.4 ± 0.37, 6.0 ± 0.15, and 4.5 ± 0.34. The positive control group was 5.5 ± 0.27, which was significantly recovered from the control group.

I. Measurement of Serum IgE and Histamine Expression

After the experiment, blood was collected from the abdomen of the animal and centrifuged at 3,000 rpm for 10 minutes. IgE (BD biosciences, USA) and histamine kit (Abnova, Taiwan) were used to measure the amount of serum IgE and histamine. Measured. The results were as shown in FIGS. 2 and 3.

As shown in Figure 2, IgE in the control group is an immunoglobulin associated with the allergic reaction, the level was increased due to the increased immune response due to atopic induction, and in the experimental group, the sample administration group and the positive control group, the level is thought to inhibit atopy It became.

When the antigen is stimulated again when the high affinity receptor and the IgE antibody of the mast cells are combined, histamine is secreted. As shown in FIG. 3, this also increases in the control group and decreases in the experimental group, the sample administration group and the positive control group. It was confirmed that it may help to reduce pruritus.

All. Determination of Alanine aminotransferase (ALT) and aspartate aminotransferase (AST)

AST and ALT are widely used in the diagnosis of liver disease. As the hepatotoxicity in serum causes necrosis of liver cells and destruction of liver tissue, the enzyme is released into the blood and its activity in plasma increases. Used as an indicator of liver damage. ALT and AST were measured using AST (GOT) and ALT (GPT) kit (Asan Pharm Co, Korea) as isolated serum, and the results are shown in FIGS. 4 and 5.

As can be seen in Figures 4 and 5, the results of the AST and ALT measurement was found that not significantly deviated from the standard of blood chemical reference indicators in all groups, it was determined that there is no liver toxicity according to the sample.

la. Hematoxylin & eosin (H & E) staining

The dorsal skin of the sacrificed animal for the experiment was taken, fixed in 10% formalin, and embedded in paraffin to make blocks. In addition, H & E staining was performed to identify epidermis and dermis causing inflammation, and it was photographed using an optical microscope (× 100, Leica, Germany). The results are shown in FIGS. 6 and 7.

6 and 7, the epidermis thickness of the control group induced by atopic dermatitis with DNCB was 78.0 ± 5.84um, compared to the normal group having an epidermis thickness of 18.6 ± 2.58um. It could be confirmed. In addition, the experimental group was thinner than the control group at 53.4 ± 6.16, 53.5 ± 2.66, and 50.7 ± 6.62um, respectively, and the positive control group was also thinner than the control group at 38.3 ± 4.41um, respectively. This confirmed that the expansion due to skin damage caused by DNCB was reduced by the treated sample.

hemp. Determination of expression levels of iNOS and COX2

iNOS and COX2 are representative proteins related to the inflammatory response and have been shown to increase the expression level in atopy. The back skin of the sacrificed animal was homogenized using ripabuffer (Thermo, USA), and 60 ug of protein was electrophoresed on a 10% SDS gel, which was transferred to a PVDF membrane (Roche, Switzerland).

After the primary antibody was reacted at 4 ° C. and washed three times using TBST, the secondary antibody was reacted at room temperature for 1 hour to confirm the band at LAS 4000 mini (GEHealthcare, USA) using ECL (Thermo, USA). It was. The results are shown in FIGS. 8 and 9.

As can be seen in Figures 8 and 9, the expression level of iNOS and COX2 was increased in the control group induced by atopic dermatitis with DNCB, it was confirmed that the concentration is inhibited in the experimental group.

bar. Statistical analysis

All data analyzes were expressed in Mean and Standard Error (SEM) using GraphPad Prism ver503, and the significance between experimental groups was verified at p <0.05 level by Newman-Keuls test according to one way ANOVA.

(Test Example 6)

In order to confirm the antioxidant activity of the food composition according to Example 1, the antibacterial activity test by DPPH METHOD was requested to the Daegu Haany University Oriental Medicine Life Research Center. As a control group, garlic, broccoli, blueberries, aronia, onions, tomatoes, and walnuts were used, and the results were calculated using vitamin C as a reference value (100%), and the results are shown in Table 5 below.

As shown in Table 5, the food composition according to Example 1, it was confirmed that the antioxidant activity is significantly superior to garlic, broccoli, blueberries, aronia, onions, tomatoes, walnuts. Therefore, it could be determined that the removal rate of the active oxygen would also be excellent.

Test Example 6 Results division result Example 1 105.430% Vitamin c 100% garlic 11.131% broccoli 43.665 & Blueberries 63.077% Aaronia 97.647% onion 4.163% tomato 5.792% Walnut 92.036%

As described above, optimal embodiments have been disclosed in the specification. Although specific terms have been used herein, they are used only for the purpose of describing the present invention and are not used to limit the scope of the present invention as defined in the meaning or claims. Therefore, those skilled in the art will understand that various modifications and equivalent other embodiments are possible. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

Claims (4)

delete 100g each of gold and silver coins, Eoseongcho, Jassop leaf, Indongcho, Gadfly, Kudji leaf, Kudji branch, Dandelion, Hagocho, Ganjacho, Machihyeon, Wasong, Seogwi, Yugeunpi, Changja, Chajeoncho, Peppermint, triticale and tanza After preparing and mixing, the mixture was pulverized to 100mesh, 20 weight times of water of the pulverized mixture was added, and then extracted and filtered at 80 ° C. for 10 hours to prevent and improve atopic dermatitis through antioxidant activity containing the extract as an active ingredient. Food composition. delete delete

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