KR102014824B1 - Health food composition for vascular relaxation and anti-thrombotic effects comprising Black Ginseng Radix, Salviae Miltiorrhizae Radix, Leonuri Herba and Cnidii Rhizoma - Google Patents

Abstract

The present invention provides a health food composition having vascular relaxation and anti-thrombotic effects containing a black ginseng extract, a Salvia miltiorrhiza extract, a Leonuri Herba extract, and a Cnidium Rhizome extract as active components. The composition according to the present invention exhibits a significantly improved vascular relaxation effect and an anti-thrombotic effect compared to an existing ginkgo leaf extract, thereby being able to be applied for food and a pharmaceutical composition for preventing or alleviating diseases related to blood circulation disturbances.

Description

Health food composition for vascular relaxation and anti-thrombotic effects comprising Black Ginseng Radix, Salviae Miltiorrhizae Radix, Leonuri Herba and Cnidii Rhizoma}

The present invention relates to a health food composition having a vascular relaxation effect and an antithrombotic effect, including black ginseng, sweet ginseng, motherwort herb and horoscope as an active ingredient.

Blood circulation is the circulation of blood through our blood vessels centered around the heart, supplying oxygen to the tissues of the body, releasing carbon dioxide, nutrients, and metabolites, and in many endocrine glands. By carrying hormones, it refers to the process of controlling the function of specific organs, controlling pathogens, controlling body temperature, controlling osmotic pressure, controlling water.

However, when blood circulation disorders occur, blood vessels extending in every corner of the body lose elasticity and cholesterol accumulates on the inner wall, narrowing the lumen of the blood vessels. It causes a lot of difficulties to lead a normal life.

According to the statistics of the World Health Organization (WHO), the mortality rate due to cardiovascular disease among the blood circulation disorders is increasing every year, especially in Asia such as Japan and Korea. These cardiovascular diseases are widely known to involve processed foods with low fat and dietary fiber content, genetic factors, smoking, drinking, high blood pressure, arteriosclerosis, diabetes, obesity, lack of exercise, and excessive stress. Looking at the development of these lesions, physical or chemical wounds cause damage to the surface of the monolayer cell of blood vessels, and thrombosis receptors are expressed on the walls of the blood vessels, whereby monocyte, T- Lymphocytes (T-lympocytes) and platelets attach and infiltrate into the vascular wall through the vascular membrane cells. At this time, lipoproteins in the blood are also introduced. They are transformed into macrophage, and macrophages accumulate cholesterol to form foam cells. The foam cells break down or thicken blood vessel walls during repeated inflammatory reactions and cell growth, resulting in adhesion of platelets in the blood. This process eventually leads to a narrowing of the vessels caused by arteriosclerosis and the complete closure of the vessels by blood clots. To the blood supply is instantaneously cut off, leading to death from heart attack.

In order to prevent or treat diseases such as numbness of the hands and feet, squeezing the neck, stiff neck, memory loss, lethargy, dizziness, chronic fatigue and hypertension and atherosclerosis caused by the blood circulation disorders, such as hydroxyprogesterone acetate, Vascular relaxants containing chemicals such as progesterone and estrogen have been used.

However, the continuous use of these chemicals increases the frequency of causing cancer and has stability problems. Recently, natural drugs derived from natural products have fewer side effects and are safer and have better effects of improving diseases caused by blood circulation disorders. Research is underway.

In this regard, the Republic of Korea Patent No. 1247633 is effective in extracting herbal medicines composed of Angelica, Red Peony, Yakja, Hyangbuja, Joiner, Safflower, Doin, Cinnamon and Licorice, which show the effect of promoting vasorelaxation, lowering blood pressure and reducing cerebral infarction. Disclosed is a composition for preventing or treating stroke comprising as an ingredient, Korean Patent No. 0948332 discloses a composition for the prevention and treatment of cardiovascular disease comprising a magpie swimming extract, Republic of Korea Patent No. 0833705 No. discloses blood circulators or vasodilators containing extracts of Oryra (Anogeissus leiocarpus), Gboigboi, Cnestis sp) or sorghum (Osusu, Drypetus molunduana) with excellent vasorelaxant effects. ,

However, by using the black ginseng, dansam, motherwort and uterus as in the present invention, hand and foot numbness, back pull, shoulder stiffness, memory loss, lethargy, dizziness, chronic fatigue and hypertension, arteriosclerosis, etc. There is no disclosure about the effect of vascular relaxation to prevent or ameliorate the disease.

The present inventors have made efforts to develop a composition for the prevention or improvement of plant-derived blood circulation disorders, which is harmless to humans and excellent in safety, using natural products. The composition to confirm that the vasorelaxant effect is remarkably excellent and completed the present invention.

1. Republic of Korea Patent No. 1247633 2, Republic of Korea Patent No. 0944332 3. Republic of Korea Patent No. 0835705

Therefore, the problem to be solved by the present invention is to provide a health food composition having a vascular relaxation effect and an antithrombotic effect comprising black ginseng, sweet ginseng, motherwort herb and cheongung as an active ingredient.

In order to achieve the above object, the present invention
Black ginseng extract;
Sweet Ginseng Extract;
Motherwort extract; And
It provides a health food composition having a vascular relaxation effect and an antithrombotic effect by including the cheonunggung extract as an active ingredient.

In the health food composition according to the present invention, the black ginseng extract may be obtained by adding an α-amylase and β-glukinase to the extract and then performing an enzymatic reaction.

In the health food composition according to the present invention, the black ginseng may be prepared as black ginseng after a pretreatment step of immersing the ginseng in hot water extract of iris.

Since the composition according to the present invention exhibits significantly improved vascular relaxation and antithrombotic effects as compared to the conventional ginkgo biloba extract, it can be applied to food and pharmaceutical compositions for the prevention or improvement of blood circulation disorders.

This will be described in detail the present invention.

The present invention relates to a composition for the prevention or improvement of blood circulation disorder disease comprising black ginseng, sweet ginseng, motherwort and cheongung as an active ingredient, wherein the black ginseng is steamed ginseng and repeated drying, sagnin Rg2, Rg3, Rh1, As Rh2 is increased, any one of the technical field to which the present invention belongs can be used without particular limitation.

In addition, the salvia is the root of Salvia miltiorrhiza Bunge (Lactaceae and Labiatae), motherwort is the ground part of Leonurus japonicus Houtt (Lactaceae and Labiatae), cheongung is the root stem of Cnidium officinale Makino (mountain and Umbelliferae), the field to which the present invention belongs As long as it is widely known, there is no restriction in particular.

The black ginseng, red ginseng, motherwort and cheongung used in the composition according to the present invention can be used in any form as long as they are well known in the art to which the present invention pertains, such as powders, extracts, and concentrates of extracts. Considering the convenience and the effect on the prevention or improvement of blood circulation disorders, all of them are extracted and then used as an active ingredient as a concentrate, and black ginseng as an extract concentrate and sweet ginseng, motherwort and cheongung in powder form. Preference is given to preparing in rings together with other excipients.

The powder refers to a powder of various forms, such as those made by drying the medicinal part and powdered by freeze-dried extract, and also a method for producing an extract of black ginseng, red ginseng, motherwort and cheongung and a concentrate thereof. The method of the present invention can be used without particular limitation as long as it is well known in the art to which the present invention pertains, and the extraction solvent is preferably purified water to ethanol because it is used as a food or pharmaceutical composition.

In particular, according to various tests of the present inventors, the black ginseng concentrate was found to be very useful for the prevention or improvement of blood circulation disorders by using the concentrated extract after treatment with starch, pectin and polysaccharide degrading enzymes, starch, pectin If it is known as a polysaccharide degrading enzyme and can be used without particular limitation.

In addition, the black ginseng used in the present invention is preferably used to prepare a pretreatment immersed in the iris extract before steaming the ginseng, which is very useful for the prevention or improvement of blood circulation disorder disease by the present inventors various tests This is because it is useful.

The pretreatment time is not particularly limited, but is preferably 10 minutes to 1 hour, and the extraction of the irises may be performed by using irises as they are, crushed irises, and extraction may be performed on powder. Extract may be added in 1 to 20 times the weight of fresh ginseng, but is not limited thereto.

The composition according to the invention has the use of preventing or ameliorating diseases caused by blood circulation disorders. In one example, the composition according to the present invention exhibits significantly improved vascular relaxation and antithrombotic effects as compared to conventional ginkgo biloba extract.

Therefore, the composition according to the present invention can be applied to various blood circulation disorders, diseases or abnormal conditions that can be prevented or treated by controlling blood circulation or vasorelaxation. For example, hypertension, diabetes mellitus, arteriosclerosis, ischemic heart disease, coronary artery disease, angina pectoris, shoulder stiffness, memory loss, lethargy, dizziness, chronic fatigue, stroke, myocardial infarction or cerebrovascular disease, but is not limited thereto. no.

As used herein, the term "prevention" means to inhibit the occurrence of a disease or condition in an individual who has not been diagnosed as having a disease or condition but is prone to such disease or condition, as used herein. "Treatment" or "improvement" means (a) inhibiting the development of a disease or disorder in a subject (b) alleviating the disease or disorder and (c) eliminating the disease or disorder. Means of mammals, such as monkeys, cows, horses, pigs, sheep, dogs, cats, rats, mice, chimpanzees and the like, including humans with a disease that can improve symptoms by administering the composition of the above.

The composition according to the invention may comprise an acceptable carrier in addition to the active ingredient. Carriers included in the composition of the present invention are commonly used in the preparation of carbohydrate compounds (eg, lactose, amylose, dextrose, sucrose, sorbitol, mannitol, starch, cellulose, etc.), acacia rubber, calcium phosphate , Alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrups, salt solutions, alcohols, gum arabic, vegetable oils (e.g. corn oil, cotton seed oil, soy milk, olive oil, coconut Oil), polyethylene glycol, methyl cellulose, methylhydroxy benzoate, propylhydroxy benzoate, talc, magnesium stearate and mineral oil, and the like.

The composition according to the present invention may further include lubricants, wetting agents, sweeteners, flavoring agents, emulsifiers, suspending agents, preservatives and the like in addition to the above components.

In addition, when the composition according to the present invention is used as a food composition, it may include components that are commonly added during food production. Examples include proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. The above-mentioned carbohydrates are monosaccharides of glucose and fructose sugars, disaccharides such as maltose, sucrose and oligosaccharides, polysaccharides such as dextrin and cyclodextrins, and sugar alcohols such as xylitol, sorbitol and erythritol. The flavourant may be a natural flavourant (tauumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavorants (saccharin, aspartame, etc.).

There is no particular limitation on the type of food. Examples of foods to which the Camellia extract may be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums and ice cream. Products, various soups, beverages, teas, drinks, alcoholic beverages and vitamin complexes, and includes all healthy foods in the usual sense.

When the food of the present invention is a beverage, various flavors or natural carbohydrates and the like may be contained as additional ingredients, as in general beverages. The natural carbohydrate may be a monosaccharide such as glucose or fructose, a disaccharide such as maltose or sucrose, and a natural sweetener such as dextrin or cyclodextrin, or a synthetic sweetener such as saccharin or aspartame. .

In addition to the above-mentioned ingredients, the food contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, And carbonating agents used in carbonated drinks. In addition, the health food of the present invention may contain fruit flesh for the production of natural fruit juices, beverages and vegetable drinks. These components can be used independently or in combination.

In the composition according to the present invention, the active ingredient consisting of the black ginseng, sweet ginseng, motherwort and cheongung may be 10 to 95% by weight, based on the total composition, and the weight ratio of black ginseng, sweet ginseng, motherwort and the gung It is most preferably included at a ratio of about 1: and is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to examples. These examples are only for explaining the present invention in more detail, and according to the gist of the present invention, the scope of the present invention is not limited to these examples. It will be apparent to those skilled in the art to which the present invention pertains.

Example  1: Preparation of the composition according to the invention

Extract black ginseng extract concentrate, sweet ginseng extract concentrate, motherwort extract concentrate, Cheongcho extract concentrate using ethanol as the extraction solvent, and concentrate them under reduced pressure to prepare each, and then mix them in a weight ratio of 4: 2: 2: 1. The composition according to the invention was prepared.

Example  2: preparation of a composition according to the invention

Same as Example 1, but in the case of black ginseng extract concentrate, the composition according to the present invention is different from that prepared by adding 0.2 wt% of α-amylase and β-glukinase to the extract and reacting for about 2 hours. Was prepared.

Experimental Example  1: Measurement of Vascular Relaxation Effect

In order to confirm the vascular relaxation effect of the compositions prepared in Examples 1 and 2, the relaxation effect of the coronary artery of the pig heart was measured.

First, the pig heart immediately after the slaughter at the slaughterhouse was purchased, and then the coronary artery was extracted and 18 mM NaCl, 47 mM KCl, 11 mM MgSO ₄ , 12 mM KH ₂ PO ₄ , 15 mM CaCl ₂ , 25 mM NaHCO ₃ , 10 mM It was put into glucose solution (Krebs) solution (pH 7.4) and the connective tissues and fats were removed, and they were made into sections of about 3 mm in length.

The coronary artery was then fixed in a 37 ° C. Krebs solution saturated with 95% O ₂ and 5% CO ₂ gas, followed by 10 minutes at 80% of vascular peak contraction with thromboxane derivative U46619 (1-60 nM). During contraction. Then, the reaction was relaxed with 300 nM of Bradykinin to measure the stability of vascular endothelial cells, and then washed three times with Krebs solution.

Then, the stability of the coronary artery was measured by U45519, and then the composition prepared in Examples 1 and 2 was treated at a concentration of 100 μg / ml to obtain an isometric tension force-displacement transducer. Vasorelaxant effect change was measured using a physiological recorder (Grass physiograph, HugoSachs, Germany) equipped with, HugoSachs, Germany.

As a control, a ginkgo leaf extract used as a conventional vasoprotectant was used. The measurement of vascular relaxation effect was repeated three times for each sample and expressed as the average and standard deviation. The ED50 value indicates the concentration of the sample (㎍ / ml) in which the contracted blood vessel shows 50% vascular relaxation by sample treatment. Turn on. The results are shown in Table 1.

ED50 (μg / ml) Control (Ginkgo Leaf Extract) 285 Example 1 18.7 2 to implementation 5.7

As shown in Table 1, the composition of Example 1 is about 15 times compared to the control (Ginkgo leaf extract), the composition of Example 2 exhibits about 50 times the vasorelaxant effect, high blood and relaxation of the composition according to the present invention Efficacy could be confirmed.

Experimental Example  2: inhibitory effect of human platelet aggregation

In order to separate platelet rich plasma (PRP) from humans, 32% sodium citrate was used as an anticoagulant and blood was drawn from a vein of a healthy male who did not take the drug for more than two weeks.

After centrifugation of 150 g of collected blood for 15 minutes, the supernatant (PRP) was obtained and the residue was centrifuged again to separate platelet poor plasma (PPP). PRP was diluted with PPP, diluted to include 1ml 3 × ^{10 8} platelets, and used for the experiment.

Platelet aggregation activity was measured by absorbance change using a platelet aggregation system (lumi-aggregometer, Chrono-Log Co, USA).

Add fermented soybean extract to a concentration of 200mg / ml to PRP pre-cultured in a thermomixer at 37 ° C for 2 minutes, incubate for 7 minutes, and put 500 μl of cultured PRP into a silicon-coated platelet aggregation cuvette. After incubation for 1 minute, collagen, which is a platelet aggregation-inducing sample, was added at a concentration of 1 to 3 µg / ml, which is the minimum concentration causing maximum aggregation, and then the reaction was observed for 6 minutes, and the results are shown in Table 2. The platelet aggregation inhibition rate (%) of Table 2 shows the comparative value when the platelet aggregation inhibition rate of the control group which processed only collagen was 0%.

Platelet aggregation inhibition rate (%) Control (Ginkgo Leaf Extract) 67.2 Example 1 31.2 2 to implementation 15.3

As shown in Table 2, the platelet aggregation inhibition rate was 2 times higher in the composition of Example 1 and 4 times higher in the composition of Example 2 than in the control group of ginkgo biloba extract, indicating that the antithrombotic efficacy of the composition according to the present invention is excellent. Could.

Accordingly, the composition according to the present invention exhibits significantly improved vascular relaxation effect and antithrombotic effect as compared to the conventional ginkgo leaf extract, and thus can be applied to food and pharmaceutical compositions for preventing or improving blood circulation disorders.

Example  3: preparation and testing of the composition according to the invention

Black ginseng used in the same manner as in Example 1 has a difference in using the one prepared after the pretreatment step of immersing the ginseng in hot water extract for 30 minutes.

The composition of Example 3 thus prepared was subjected to the same test as in Experimental Examples 1 and 2, and the results are shown in Table 3.

Example 3 ED50 (μg / ml) 10.2 Platelet aggregation inhibition rate (%) 22.8

Looking at the results of Table 3, it was confirmed that all improved than the results of Example 1, pretreatment using the iris extract improved the vascular relaxation effect and antithrombotic effect.

Claims (6)

Black ginseng extract;
Sweet Ginseng Extract;
Motherwort extract; And
Health food composition having vascular relaxation effect and antithrombotic effect, including the extract of the cheongung as an active ingredient.
The method of claim 1,
The black ginseng extract is a health food composition having a vascular relaxation effect and antithrombotic effect, characterized in that obtained by the enzyme reaction after the addition of α-amylase and β-glukinase to the extract.
The method of claim 1, wherein the black ginseng for preparing the black ginseng extract is a health food composition having a vascular relaxation effect and anti-thrombotic effect, characterized in that the black ginseng prepared after the pretreatment step immersed in iris hot water extract.

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