KR101982175B1 - Fat SVF(stromal vascular fraction) extracting apparatus and fat SVF(stromal vascular fraction) extracting method using the same - Google Patents

Abstract

The present invention provides a method for producing a lipid-lipolytic enzyme, comprising: a first chamber in which a mixture of fat and lipolytic enzymes is centrifuged; A second chamber detachably coupled to the first chamber, wherein the high-density material supplied from the first chamber is cleaned and centrifuged by the expansion liquid; A third chamber that is detachably coupled to the second chamber to filter out stromal vascular fraction (SVF) by subjecting the high-density substance supplied from the second chamber to centrifugal separation; And a centrifuge for rotating the first chamber, the second chamber, and the third chamber to centrifuge the material inside the first chamber, the second chamber, and the third chamber, and a fat using the same Thereby providing a substrate cell extraction method.
According to the apparatus for extracting fat cells and extracting fat cells using the same according to the present invention, the recovery rate of fat cells can be improved by performing the centrifugation through the first, second, and third chambers in multiple steps. , It is possible to reduce the extraction time of fat stromal cells and the cost of extracting fat stromal cells.
In addition, according to the present invention, it is possible to assemble and separate the first, second, and third chambers, thereby reducing the total length of the chamber, Conventional type centrifuges can be used for the stromal cell extractor.
In addition, according to the apparatus for extracting lipid stromal cells according to the present invention and the method for extracting lipid stromal cells using the same, it is possible to filter out impurities that may be generated in the extraction of stromal cells through a filter network built in the lower part of the third chamber, It is possible to prevent a physiological problem that may occur when the completed stromal cells are injected into the blood vessels of the human body.

Description

(Stromal Vascular Fraction Extraction Apparatus and Fat SVF (Stromal Vascular Fraction) Extraction Method Using the Same}

More particularly, the present invention relates to a device for extracting stromal cells existing in an adipose tissue through centrifugation and a method for extracting fat stromal cells using the same .

Generally, SVF (Stromal Vascular Fraction) refers to regenerative healing cells obtained from adipose tissue of the human body. The stromal cells are also referred to as substrate blood vessel fraction or adipose tissue-derived cell substrate fraction, and have adipocyte stem cells and fibroblasts, immune cells, and vascular endothelial cells.

In recent years, treatment with fatty stromal cells has been frequently performed in an anti-aging clinic for cosmetic surgeons. It is used in the form of extracting the fat of the patient, extracting the fat cell matrix rich in stem cells from the fat, and transplanting it into the patient's body through intravenous injection or implantation according to the patient's disease / treatment purpose.

The most important part in extracting the stromal stromal cells is to prevent the fat from being exposed to the external environment in the process of extracting the stromal stromal cells to prevent contamination of the adipose tissue. Therefore, it is important to improve the recovery rate of fat stromal cells and decrease the extraction time of fat stromal cells, thereby reducing the cost of extracting fat stromal cells.

Korean Patent No. 10-1440754 discloses a cell-concentrated separator and a separation method related to the extraction of such stromal stromal cells.

The conventional separator provides a separator in which an upper body and a lower body are integrally provided. At this time, the conventional separator has a problem that the recovery process of fat stromal cells is low because the separation process of fat stromal cells by centrifugation is not provided in multiple stages.

DISCLOSURE Technical Problem Accordingly, the present invention has been made in an effort to solve the above problems, and it is an object of the present invention to provide an improved fatty stomach cell extracting apparatus capable of separating stromal cells from adipose tissue through various stages of centrifugation, And a method for extracting fat stromal cells using the same.

An apparatus for extracting fat cells according to one aspect of the present invention includes: a first chamber in which a mixture of fat and lipolytic enzymes is centrifuged; A second chamber detachably coupled to the first chamber, wherein the high-density material supplied from the first chamber is cleaned and centrifuged by the expansion liquid; A third chamber that is detachably coupled to the second chamber to filter out stromal vascular fraction (SVF) by subjecting the high-density substance supplied from the second chamber to centrifugal separation; And a centrifuge for rotating the first, second, and third chambers to centrifuge the materials within the first chamber, the second chamber, and the third chamber.

The apparatus for extracting fat and stromal cells may further include a first inlet installed at an upper portion of the first chamber and introducing a mixture of fat and collagenase into the first chamber, And a first cannula for extracting a low-density substance from the mixture remaining in the first chamber after centrifugal separation.

The apparatus for extracting fat cells comprises a second inflow section provided at a side of the second chamber and through which saline solution is introduced into the second chamber and a second inflow section provided at the second inflow section, And a second cannula for extracting a low-density substance from the remaining mixture.

Wherein the lipid stromal cell extracting device comprises a pair of first engaging portions which are respectively installed on the lower portion of the first chamber and on the upper portion of the second chamber and which are coupled to each other to allow the first chamber and the second chamber to communicate with each other, A pair of second engaging parts which are respectively installed on the lower part of the second chamber and on the upper part of the third chamber and which are coupled to each other to allow the second chamber and the third chamber to communicate with each other, The first valve selectively opens and closes the first engaging part, and the second valve selectively opens and closes the second engaging part.

The apparatus for extracting fat and stromal cells according to the present invention comprises a partition wall having an upper space and a lower space separated from each other by an inner space of the third chamber and having a communication hole communicating the upper space and the lower space, And a filter net installed in the third chamber to filter only the stromal cells of the mixture in the third chamber into the lower space.

The lipid stromal cell extracting apparatus may further include a discharging unit provided at a lower portion of the third chamber and a lure lock syringe installed at the discharging unit for extracting stromal cells present in the lower space .

The centrifuge may receive and rotate the first chamber and the second chamber coupled to each other or the second chamber and the second chamber coupled to each other.

According to another aspect of the present invention, there is provided a method for producing a lipid-lipolytic enzyme, comprising: supplying a mixture of fat and lipolytic enzyme into a first chamber; Centrifuging the mixture within the first chamber; Supplying a high density material in the mixture within the first chamber to the second chamber; Supplying an expansion liquid into the second chamber to clean the mixture inside the second chamber; Centrifuging the mixture within the second chamber; Supplying a high density material in the mixture within the second chamber to the third chamber; Centrifuging the mixture in the third chamber, and filtering the stromal vascular fraction (SVF) in the mixture in the third chamber.

The method of claim 1, further comprising: coupling the first chamber and the second chamber to each other; and after locking the first valve between the first and second chambers, introducing a mixture of fat and collagenase into the interior of the first chamber Can be supplied.

Centrifuging the mixture within the first chamber may accommodate and rotate the first chamber and the second chamber within the centrifuge.

The method further comprises centrifuging the mixture in the first chamber and then extracting a low density substance from the mixture in the first chamber by centrifugation into a first cannula can do.

The method comprising: coupling a third chamber to the second chamber; and closing the second valve between the second chamber and the third chamber to open the first valve to allow material remaining in the first chamber to pass through the second chamber, .

The method further comprises separating the first chamber from the second chamber after the material inside the first chamber is supplied to the second chamber, Wherein the step of flushing is to inflate the fat by supplying saline to the interior of the second chamber, wherein centrifuging the mixture within the second chamber comprises: accommodating the second and third chambers in a centrifuge .

The method further comprises centrifuging the mixture in the second chamber and extracting a low density material from the mixture in the second chamber by centrifugal separation into a second cannula, The step of filtering the stromal cells in the mixture in the third chamber may include filtering the stromal cells only into the lower space by the filter net installed on the upper side of the partition dividing the inner space of the third chamber into the upper space and the lower space .

The lipid stromal cell extract method may further include extracting stromal cells by inserting stromal cells into the lower space and inserting a lure-lock syringe into the lower space.

According to the apparatus for extracting fat cells and extracting fat cells using the same according to the present invention, the recovery rate of fat cells can be improved by performing the centrifugation through the first, second, and third chambers in multiple steps. , It is possible to reduce the extraction time of fat stromal cells and the cost of extracting fat stromal cells.

In addition, according to the present invention, it is possible to assemble and separate the first, second, and third chambers, thereby reducing the total length of the chamber, Conventional type centrifuges can be used for the stromal cell extractor.

In addition, according to the apparatus for extracting lipid stromal cells according to the present invention and the method for extracting lipid stromal cells using the same, it is possible to filter out impurities that may be generated in the extraction of stromal cells through a filter network built in the lower part of the third chamber, It is possible to prevent a physiological problem that may occur when the completed stromal cells are injected into the blood vessels of the human body.

FIG. 1 is an exploded view of a first chamber, a second chamber, and a third chamber included in an apparatus for extracting lipid stromal cells according to an aspect of the present invention.
FIG. 2 is a view showing a state where the first chamber and the second chamber according to FIG. 1 are accommodated in the centrifugal separator.
FIG. 3 is a view showing a state in which the second chamber and the third chamber according to FIG. 1 are accommodated in the centrifugal separator.
FIG. 4 is a flowchart illustrating a method of extracting a fat stromal cell according to another aspect of the present invention.

While the present invention has been described with reference to exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. Therefore, the true scope of the present invention should be determined by the technical idea of the appended claims.

Hereinafter, a fat stomach cell extracting apparatus and a fat stomach cell extracting method using the same according to an embodiment of the present invention will be described in detail with reference to the drawings.

FIG. 1 is an exploded view of a first chamber, a second chamber, and a third chamber included in an apparatus for extracting lipid stromal cells according to an aspect of the present invention. FIG. 2 is a cross- FIG. 3 is a view showing a state where the second chamber and the third chamber according to FIG. 1 are accommodated in the centrifugal separator. FIG.

1, an apparatus 100 for extracting lipid stromal cells according to an aspect of the present invention includes a first chamber 110, a second chamber 120, a third chamber 130, Separator < / RTI > The first chamber 110 receives a mixture of fat and lipolytic enzymes therein. The lipolytic enzyme is intended to decompose fat to facilitate separation of stromal vascular fraction (SVF) from adipose tissue, and may be typified by collagenase. Collagenase breaks down peptide bonds in the collagen network that maintains adipose tissue and destroys the basement membrane around individual cells.

A first inlet 111 may be provided in the upper portion of the first chamber 110. The mixture of fat and collagenase is introduced into the first chamber 110 through the first inlet 111. Also, a first cannula 112 (Cannula) may be installed in the first inlet 111. The first cannula 112 extracts a low density material from the first chamber 110 from the remaining mixture after the mixture is centrifuged in the first chamber 110. At this time, the first cannula 112 is not always installed in the first inlet 111, and the first chamber 110 rotates inside the centrifuge 140, 140, a low-density substance is extracted from the first inlet 111.

The second chamber 120 is detachably coupled to the first chamber 110, and the high-density material supplied from the first chamber 120 is washed and centrifuged by the expansion liquid. That is, after the low density material is extracted from the first chamber 110 by the first cannula 112, the high density material remaining in the first chamber 110 is transferred to the second chamber 120 . At this time, the inflating liquid may be physiological saline, and a second inflow part 121 through which the physiological saline solution flows into the second chamber 120 may be installed on the side of the second chamber 120. This physiological saline solution is used to expand and stabilize adipose tissue so that stromal cells can be easily separated from adipose tissue.

Also, a second cannula 122 may be installed in the second inlet 121. The second cannula 122 extracts a low-density material from the second chamber 120, which is remaining in the interior of the second chamber 120 after centrifugal separation. Of course, the second cannula 122 at this time is also connected to the second inlet 121 after centrifugal separation of the mixture in the second chamber 120, like the first cannula 112 This corresponds to a configuration that is temporarily installed.

The third chamber 130 is detachably coupled to the second chamber 120, and the high-density material supplied from the second chamber 120 is centrifuged to filter out stromal cells. The third chamber 130 may be provided with a partition 131, a filter net 132, a discharge unit 133, and a lure-lock syringe 134.

The partition 131 is installed inside the third chamber 130 so as to cross the third chamber 130 laterally. The partition 131 divides the inner space of the third chamber 130 into an upper space 130a and a lower space 130b. The filter net 132 is installed in the upper space 130a and filters only stromal cells among the substances centrifuged in the third chamber 130 into the lower space 130b. More specifically, a communicating hole 131a is formed in the center of the partition 131 so as to pass through the upper space 130a and the lower space 130b. The filter network 132 is provided on the partition wall 131 so as to cover the communication hole 131a so that other unnecessary cells including fibrous cells are left in the upper space 130a, To be filtered into the lower space 130b.

The discharge part 133 is installed at the lower part of the third chamber 130 and communicates with the lower space 130b. The luer lock syringe 134 is installed in the discharge part 133 and extracts stromal cells filtered by the lower space 130b from the third chamber 130. Like the first and second cannulas 112 and 122, the luer lock syringe 134 is connected to the discharge port of the third chamber 130 after the centrifugal separation of the mixture in the third chamber 130 is completed. 133, respectively.

A pair of first engaging portions 150 are provided between the first chamber 110 and the second chamber 120 and a pair of second engaging portions 150 are provided between the second chamber 120 and the third chamber 130. [ The second engaging portion 160 of the engaging portion 160 may be provided. 1 and 2, the lower portion of the first chamber 110 is formed to have a gradually decreasing width in a downward direction, and the lower portion of the second chamber 120, The width of the upper portion is gradually reduced toward the upper side. The pair of first coupling portions 150 are installed at the lower portion of the first chamber 110 and the upper portion of the second chamber 120, respectively. 1 and 3, the lower part of the second chamber 120 is formed to have a gradually decreasing width in the lateral direction, and the upper part of the third chamber 130 , And the width in the lateral direction gradually decreases toward the upper side. The pair of second engaging portions 160 are installed in the lower portion of the second chamber 120 and the upper portion of the third chamber 130, respectively. The first and second chambers 110 and 120 are coupled to each other so that the pair of second couplings 160 can communicate with each other. The chamber 120 and the third chamber 130 are coupled to each other to communicate with each other.

The pair of first coupling portions 150 may be coupled and separated from each other in a tapping manner. Accordingly, the pair of first engaging portions 150 can improve the ease of assembling the first chamber 110 and the second chamber 120.

A first valve 151 may be installed in the pair of first coupling parts 150 and a second valve 161 may be installed in the pair of second coupling parts 160. As shown in the drawing, the first valve 151 may be installed on the side of the second chamber 120 of the pair of first engaging portions 150, and the second valve 161 may be provided on the side of the second chamber 120, The first valve 151 and the second valve 161 may be mounted on the third chamber 130 side of the pair of second coupling portions 160. However, May be installed in the other one.

The centrifuge 140 rotates the first chamber 110, the second chamber 120 and the third chamber 130 to rotate the first chamber 110, the second chamber 120, And centrifuge the material inside the chamber 130. When the drawing is taken as a reference, the upper side becomes the rotation center, and the centrifugal force can be directed to the lower side based on this.

At this time, the centrifugal separator 140 includes only the first chamber 120 and the third chamber 130 coupled to each other, or only the second chamber 120 and the third chamber 130 coupled to each other, And can be rotated. That is, not only the first chamber 110, the second chamber 120, and the third chamber 130 are coupled, but only two adjacent chambers are coupled to the centrifuge 140, And the centrifuge 140 may also be configured to accommodate only two adjacent chambers 110, 120, and 130, but not both, of the first, second, and third chambers 110, It can be operated to rotate simultaneously.

In order to accommodate both the first chamber 110, the second chamber 120, and the third chamber 130, the manufacturing cost of the centrifuge 140 increases. When the first chamber 110, the second chamber 120, and the third chamber 130 are both housed in the centrifuge 140, the third chamber 130 is far away from the center of rotation So that an excessively large centrifugal force is applied to the third chamber 130. In this case, the pair of first engaging portions 150 and the second engaging portions 160 can be separated from each other without maintaining the fixed coupling state, which can cause damage or destruction of the apparatus. Accordingly, the centrifugal separator 140 receives and rotates only two adjacent ones of the chambers 110, 120 and 130.

Hereinafter, a method of extracting fat stromal cells using the extraction device 100 will be described in detail.

FIG. 4 is a flowchart illustrating a method of extracting a fat stromal cell according to another aspect of the present invention.

Referring to FIG. 1, the first and second chambers 110 and 120 are coupled to each other by coupling the pair of first coupling parts 150. After the first valve 151 is closed, a mixture of fat and collagenase is supplied into the first chamber 110 (S10). In this case, since the first valve 151 is locked, the mixture supplied into the first chamber 110 is present only in the first chamber 110, and the second chamber 120 ).

Next, the first chamber 110 and the second chamber 120 are accommodated in the centrifuge 140. The centrifuge 140 is operated to rotate the first chamber 110 and the second chamber 120 so that the mixture in the first chamber 110 is centrifuged (S20). Of course, at this time, the first inflow portion 111 is kept closed by a separate cap (not shown). Also, the second coupling part 160 provided in the second chamber 120 is also kept in a closed state by a separate cap (not shown). After the centrifugation of the mixture in the first chamber 110 is completed, the first chamber 110 and the second chamber 120 are separated from the centrifuge 140, and the first chamber 110 ) Is extracted with the first cannula (112).

Thereafter, the pair of second engaging portions 160 are coupled to each other to fix the third chamber 130 to the second chamber 120, and the second valve 161 is locked. In this state, the first valve 151 is opened to supply the high-density material remaining in the first chamber 110 to the second chamber 120 (S30). After the material in the first chamber 110 is supplied to the second chamber 120, the first chamber 110 is separated from the second chamber 120.

Next, physiological saline solution is supplied into the second chamber 120 through the second inlet 121 (S40). The second chamber 120 and the third chamber 130 are accommodated in the centrifuge 140 and rotated after a sufficient time has elapsed so that the fat tissue can be swollen in the physiological saline solution. Thereby allowing the mixture in the second chamber 120 to be centrifuged (S50). Of course, at this time, the second inflow portion 121 and the discharge portion 133 are kept closed through a separate cap (not shown).

After the second chamber 120 and the third chamber 130 are rotated sufficiently, the second chamber 120 and the third chamber 130 are separated from the centrifuge 140, Density material in the mixture in the second chamber 120 is extracted from the second chamber 120 using the first chamber 122 and the second chamber 120, respectively. In step S60, the second valve 161 is opened to supply a high-density material, which is left in the second chamber 120, to the third chamber 130.

Thereafter, the third chamber 130 is accommodated in the centrifuge 140 and rotated. Accordingly, the mixture in the third chamber 130 is centrifuged so that the stromal cells are filtered into the lower space 130b in the third chamber 130 (S70). After the stromal cells are sufficiently filtered into the lower space 130b, the cap covering the discharging part 133 is opened and the Lure rock syringe 134 is inserted into the lower space 130b to extract stromal cells, Thereby completing the extraction of fat stromal cells according to the invention.

As described above, according to the fat stomal cell extracting apparatus 100 and the fat stomach cell extracting method using the same according to the present invention, centrifugal separation is performed through the first, second, and third chambers 110, 120, , It is possible to improve the recovery rate of fat stromal cells and decrease the extraction time of fat stromal cells, thereby reducing the cost of extracting fat stromal cells.

In addition, according to the present invention, it is possible to assemble and separate the first, second, and third chambers, thereby reducing the total length of the chamber, Conventional type centrifuges can be used for the stromal cell extractor.

In addition, according to the apparatus for extracting lipid stromal cells according to the present invention and the method for extracting lipid stromal cells using the same, it is possible to filter out impurities that may be generated in the extraction of stromal cells through a filter network built in the lower part of the third chamber, It is possible to prevent a physiological problem that may occur when the completed stromal cells are injected into the blood vessels of the human body.

100: local stomach cell extracting apparatus 110: first chamber
120: second chamber 130: third chamber
140: Centrifuge

Claims (15)

A first chamber having a shape in which a mixture of fat and lipolytic enzymes is centrifuged and whose width in the transverse direction gradually decreases as the lower portion is lowered;
Wherein the first chamber is detachably attached to the first chamber and the high density material supplied from the first chamber is cleaned and centrifugally separated by the expansion liquid, 2 chambers;
A third chamber that is detachably coupled to the second chamber to filter out stromal vascular fraction (SVF) by subjecting the high-density substance supplied from the second chamber to centrifugal separation; And
And a centrifuge for rotating the first chamber, the second chamber and the third chamber to centrifuge the material inside the first chamber, the second chamber and the third chamber,
A pair of first engaging portions provided on the lower portion of the first chamber and on the upper portion of the second chamber and connected to each other to allow the first chamber and the second chamber to communicate with each other, A pair of second engaging portions that are respectively installed on the upper portion of the first chamber and are connected to each other to communicate the second chamber and the third chamber,
A first valve installed in the first coupling portion and selectively opening and closing the first coupling portion and a second valve provided in the second coupling portion and selectively opening and closing the second coupling portion,
Wherein the first chamber and the second chamber are connected to each other and the first valve is closed, and the first chamber and the second chamber are accommodated in the centrifuge, rotated and separated from the centrifuge,
The second chamber and the third chamber are connected to each other so that the second valve is closed while the first valve is opened and the first chamber is separated from the second chamber, And is rotated,
A first inlet installed in the upper portion of the first chamber, through which the mixture of fat and collagenase is introduced into the first chamber,
Further comprising a first cannula disposed in the first inlet and adapted to extract a low density of material remaining in the first chamber after rotation of the first chamber and the second chamber,
A second inflow portion provided at a side of the second chamber and into which the saline solution flows,
Further comprising a second cannula installed in the second inlet for extracting a low density of material remaining in the second chamber after rotation of the second chamber and the third chamber,
The third chamber is provided inside the third chamber so as to transverse the third chamber and the inner space of the third chamber is divided into an upper space and a lower space and a communication hole communicating with the upper space and the lower space is formed A partition wall,
And a filter net installed in the upper space to cover the communication hole and filtering only the stromal cells in the mixture of the third chamber into the lower space. delete delete delete delete The method according to claim 1,
A discharge section provided at a lower portion of the third chamber,
And a lure-lock syringe installed in the discharging unit for extracting stromal cells present in the lower space. delete Coupling a first chamber formed in a shape in which a lateral width gradually decreases toward a lower side and a second chamber formed in a shape in which an upper portion is gradually reduced in width in a lateral direction toward the upper side;
Locking a first valve between the first chamber and the second chamber;
Feeding a mixture of fat and lipolytic enzymes into the first chamber;
Centrifuging the mixture in the first chamber by receiving and rotating the first chamber and the second chamber inside the centrifuge;
Centrifuging the mixture in the first chamber and extracting a low density material from the mixture inside the first chamber centrifuged into a first cannula;
Coupling a third chamber to the second chamber;
Locking a second valve between the second chamber and the third chamber;
Opening the first valve to supply a high density of the mixture within the first chamber to the second chamber;
Separating the first chamber from the second chamber;
Supplying an expansion liquid into the second chamber to clean the mixture inside the second chamber;
Centrifuging the mixture in the second chamber by receiving and rotating the second chamber and the third chamber inside the centrifuge;
Centrifuging the mixture in the second chamber, and extracting a low density material from the mixture in the second chamber by centrifugal separation into a second cannula;
Supplying a high density material in the mixture within the second chamber to the third chamber;
Centrifuging the mixture in the third chamber to filter stromal vascular fraction (SVF) in the mixture within the third chamber,
The step of filtering the stromal cells in the mixture in the third chamber may include the step of separating the inner space of the third chamber into the upper space and the lower space so as to cross the third chamber laterally, And a filter net installed in the upper space to cover the communication holes and filtering only the stromal cells in the mixture of the third chamber into the lower space, , So that only the stromal cells are filtered into the lower space. The method of claim 8,
Wherein the lipolytic enzyme is collagenase. ≪ RTI ID = 0.0 > 21. < / RTI > delete delete delete The method of claim 8,
Wherein the step of washing the mixture in the second chamber comprises supplying the saline solution into the second chamber to expand the fat. delete The method of claim 8,
Further comprising the step of extracting stromal cells by filtering stromal cells into the lower space and then inserting a lure-lock syringe into the lower space.

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