KR101979761B1 - Composition containing Lactobacillus plantarum HY7715 for preventing from skin damage and improving skin health against fine dust as effective component - Google Patents

Abstract

The present invention relates to a composition for preventing and alleviating skin damage caused by fine dust, containing lactobacillus plantarum HY7715 as an active ingredient, which has an effect of preventing and alleviating skin damage caused by fine dust including polycyclic aromatic hydrocarbon since lactobacillus plantarum HY7715 reduces expression of aryl hydrocarbon receptor (AhR), and cytochrome P450 1A1 (CYP1A1) genes, thereby being used as a food composition and a cosmetic composition, such as fermented milk, health functional food, functional beverage, etc. which uses the same as an active ingredient.

Description

[0001] The present invention relates to a composition for preventing and improving damage to skin caused by fine dust containing Lactobacillus plantarum HY7715 as an active ingredient.

The present invention relates to a composition for preventing and improving damage to skin caused by fine dust containing Lactobacillus plantarum HY7715 as an active ingredient. More particularly, the present invention relates to a composition for preventing and / ) And CYP1A1 (Cytochrome P450 1A1) genes, thereby preventing and improving damage to the skin caused by fine dusts. The present invention relates to a method for treating skin caused by fine dusts containing Lactobacillus plantarum HY7715 as an active ingredient, And more particularly to a composition for preventing and improving the damage of the skin.

Fine dust is defined as dust that is floating in the air that is too small to be seen, less than 10 μm of total suspended particles (TSP) with an aerodynamic diameter of 50 μm or less. It is divided into fine dust (PM10) with diameter smaller than 10μm and ultra fine dust (PM2.5) with smaller diameter than 2.5μm. Since fine particles are small in size by about one-fifth of the pores of the skin, penetration of fine dust into the skin damages the protective function of the skin's outer layer and makes it susceptible to irritation. This can lead to skin barrier function deterioration, reduced elasticity, and acne. In particular, polyaromatic hydrocarbons in fine dusts enter the skin through skin follicles to increase melanocyte and pigment spots, increase collagen synthesis and degradation, and reduce wrinkle formation and elasticity to promote skin and aging do.

Probiotics are defined as live microorganisms that help the host's health when consumed in sufficient quantities by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO) Lactobacillus ( L. acidophilus , L. casei, L. gasseri, L. delbruekii subsp. Bulgaricus, L. helveticus, L. fermentum, L. paracasei, L. plantarum, L. reuteri, L. rhamnosus and L. salivarius ), one Lactococcus species ( L. lactis ), two Enterococcus species ( E. faecium, E. faecalis ) there are, Streptococcus (Streptococcus) 1 jong (S. thermophilus), Bifidobacterium (Bifidobacterium) 4 jong (B. bifidum, B. breve, B. longum, B. animalis subsp. lactis).

In particular, Lactobacillus plantarum is a lactic acid bacterium having the broadest distribution in nature, separated mainly from kimchi, pickles, and the like, and is known to have strong acid and bile acid resistance and antioxidant and anti-inflammatory effects. However, No studies have been reported on the effects of microdirt on skin damage.

Accordingly, the present inventors have found that Lactobacillus plantarum HY7715 has an effect of inhibiting the generation of active oxygen due to fine dust and controlling the expression of related genes, thereby preventing and improving the damage of human skin cells Thereby completing the present invention.

Korean Patent Publication No. 10-2017-0059615 (May 31, 2017)

The present invention relates to a pharmaceutical composition for reducing the expression of AhR (Aryl hydrocarbon receptor) and CYP1A1 (Cytochrome P450 1A1) gene in human skin cells, and to provide a method for inhibiting the damage of skin caused by fine dust containing polycyclic aromatic hydrocarbons It is an object of the present invention to provide a composition for preventing and improving skin damage caused by fine dust containing Lactobacillus plantarum HY7715 as an active ingredient.

In order to achieve the above object, the present invention relates to a pharmaceutical composition comprising an AhR (Aryl hydrocarbon receptor) and CYP1A1 (Cytochrome P450 1A1) associated with skin damage caused by treating microdirts containing polycyclic aromatic hydrocarbons in human keratinocytes (HaCaT) ) Lactobacillus plantarum HY7715, which has the effect of preventing and improving skin damage caused by fine dusts by reducing the expression of genes, is used as an active ingredient to prevent and improve skin damage caused by fine dusts ≪ / RTI >

Hereinafter, the present invention will be described in detail.

Fine particles according to the invention was used for the ERM ^® -CZ100 prepared in European Reference Materials. The sample is PM10, which is fine dust separated from the actual road environment. These samples include Benzo [a] anthracene, Benzo [a] pyrene, Benzo [b] fluoranthene, Benzo [j] fluoranthene, Benzo [k] fluoranthene, Dibenzo [a, h] anthracene, , d] pyrene.

In order to isolate Lactobacillus plantarum HY7715 according to the present invention, it is necessary to isolate Lactobacillus plantarum HY7715 from Bongryong, Chuncheon, Seochun, Jeonbuk, Chonbuk, Chonnam, Jeonnam, Gohung, Yeosu, Dolsan, Jeonnam, Boseong, , Seochung, Gochang, Goheung, and others. The fermented food such as kimchi prepared by the seller was collected, and the broth was mixed with glycerol and stored at -80 ℃ until the isolate. In order to separate the strain from the glycerol stock of the collected sample, 900 μl of the aerobic diluent and 100 μl of the glycerol stock of the fermented food sample were mixed to perform serial dilution. The 10 ^-1 to 10 ^-6 dilution of the sample plate (plate) 100㎕ per Bromcresol Purple (Bromocresol Puple) telling flatness in the MRS-agar medium containing the cells were cultured for 48 hours or more at 37 ℃. A single colony was obtained by subculturing the yellow microorganism colony formed after cultivation by sterilization loop and carrying out subculture three times at the same temperature and at the same temperature condition. The mycological characteristics of Lactobacillus plantarum HY7715 thus isolated are as follows.

1) The morphology of bacteria (characteristics of bacteria when cultured on MRS agar plate at 37 ℃ for 2 days)

① Cell shape: Bacillus

② Mobility: None

③ Spore forming ability: None

④ Gram staining: positive

2) Morphology of bacteria (characteristics of bacteria when cultured at 37 ℃ for 2 days on MRS agar plate medium)

① Shape: Circular

② Bump: convex

③ Surface: smooth

3) Physiological properties

① Growth temperature: Growth possible Growth temperature 13 ~ 43 ℃, Optimum growth temperature 33 ~ 37 ℃

② Growth pH: Growable growth pH 3.5 ~ 3.7, Optimum pH 5.0 ~ 5.5

③ Effect on oxygen: Tumor anaerobic

④ Catalase: -

⑤ Whether gas is formed: -

⑥ Growth at 15 ℃: +

⑦ Growth at 45 ℃: -

⑧ Indole production: -

⑨ Lactic acid production: +

⑩16S rDNA analysis

The strain of the present invention was identified by conducting a molecular genetic method through 16S rDNA analysis. The results of 16S rDNA sequencing are shown in Table 1 (http://www.ncbi.nlm.nih.gov/blast).

As can be seen in the following Table 1, the 16S rRNA gene of Lactobacillus plantarum HY7715 of the present invention was found to be 99% identical to the 16S rRNA gene of Lactobacillus plantarum .

Description Max score Total score Query cover Max
ident Accession Lactobacillus plantarum strain HFC8,
complete genome 2641 13137 100% 99 CP012650.1

Based on the above 16S rRNA sequence and mycological characteristics, the strain of the present invention was named Lactobacillus plantarum HY7715 and deposited on August 8, 2016 with the Korea Research Institute of Bioscience and Biotechnology (Accession No .: KCTC 13101BP ).

The food composition containing the Lactobacillus plantarum HY7715 live cell of the present invention or the lysate thereof as an active ingredient can be used as food, food additive, beverage, beverage additive, fermented milk, health functional food, and the like.

Particularly, the fermented milk containing the Lactobacillus plantarum HY7715 live bacterium of the present invention or the lysate thereof as an active ingredient is obtained by homogenizing the lyophilized powder of probiotics, the culture liquid of lactic acid bacteria and the mixed fruit syrup at a certain ratio at 150 bar After cooling to below 10 ° C, it is packaged in a container to produce fermented milk.

The functional beverage containing the Lactobacillus plantarum HY7715 live bacterium of the present invention or the lysate thereof as an active ingredient is prepared by combining the mixed fruit juice syrup, freeze-dried powder of probiotics and water at a constant ratio and homogenizing at 150 bar After cooling to 10 ° C or less, functional beverages are prepared by packaging in small containers such as glass bottles and plastic bottles.

In addition, the health functional food containing the Lactobacillus plantarum HY7715 live bacterium or the lysate thereof as an active ingredient of the present invention contains not only lyophilized powder of probiotics but also vitamins B ₁ , B ₂ , B ₅ , B ₆ , E and acetic acid esters, nicotinic acid amides, oligosaccharides and the like may be added, and other food additives may be added.

On the other hand, the cosmetic composition containing the Lactobacillus plantarum HY7715 live bacterium of the present invention or the lysate thereof as an active ingredient may be prepared by mixing Lactobacillus plantarum HY7715 with other cosmetics, Materials can be added. For example, purified water, an oil, a surfactant, a moisturizer, a high alcohol, a thickener, a chelating agent, a pigment, a fatty acid, an antioxidant, an antiseptic, a wax, a pH adjusting agent and a perfume may be added.

Further, the formulation of the cosmetic composition containing the Lactobacillus plantarum HY7715 live bacterium of the present invention or the lysate thereof as an active ingredient is not particularly limited, but for example, nutritional cream, convergent lotion, , Essence, nutritional gel, massage cream, mask pack, and the like.

The Lactobacillus plantarum HY7715 of the present invention reduces the expression of AhR (Aryl hydrocarbon receptor) and CYP1A1 (Cytochrome P450 1A1) genes, thereby preventing and improving skin damage due to micro dusts containing polycyclic aromatic hydrocarbons It can be used as a food composition and a cosmetic composition such as fermented milk, health functional food, functional beverage and the like as an effective ingredient.

FIG. 1 is a graph showing the effect of Lactobacillus plantarum HY7715 according to the present invention on keratinocyte-killing keratinocytes (HaCaT).
FIG. 2 is a graph showing the expression of the AhR gene by fine dust of Lactobacillus plantarum HY7715 of the present invention in human keratinocytes (HaCaT).
FIG. 3 is a graph showing the expression of the CYP1A1 gene by fine dust of Lactobacillus plantarum HY7715 of the present invention in human keratinocytes (HaCaT).

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following embodiments are not intended to limit the scope of the present invention, and ordinary variations by those skilled in the art are possible within the scope of the technical idea of the present invention.

≪ Example 1 >

Lactobacillus planta room ( Lactobacillus plantarum ) HY7715 Freeze-dried powder manufacture

Lactobacillus plantarum HY7715 was cultured in an MRS broth medium, and then the culture broth was centrifuged at 8,000 rpm for 15 minutes to obtain a probiotic concentrate. The resulting concentrate was coated with 10% by weight of pressurized sterilized milk powder Was mixed in a weight ratio of 1: 1, and then freeze-dried powder was prepared by freezing at -70 ° C for 6 hours.

≪ Example 2 >

Lactobacillus planta room ( Lactobacillus plantarum Production of fermented milk containing HY7715 as an active ingredient

The lactic acid bacteria culture solution was prepared by stirring 95.36% by weight of crude oil and 4.6% by weight of skimmed milk powder (or mixed powdered milk) and having a specific gravity of 1.0473 to 1.0475 at 15 ° C, a titratable acidity of 0.200 to 0.220%, a pH of 6.55 to 6.70, (Brix 0) was about 16.3 to 16.5%. After mixing, the mixture was heat-treated with UHT (sterilized at 135 ° C. for 2 seconds), cooled to an appropriate temperature, added with 0.02% by weight of Streptococcus thermophilus and Lactolytic enzyme (Valley laboratory, USA) The total number of lactic acid bacteria in the medium was 1.0 X 10 ⁹ cfu / ml or more, the titratable acidity was 0.89 to 0.91%, and the pH was 4.55 to 4.65.

The mixed juice syrup was prepared by mixing 13 wt% of liquid fructose, 5 wt% of white sugar, 10.9 wt% of concentrated juice concentrate 56Brix ⁰ , 1.0 wt% of pectin, 0.1 wt% of fresh fruit mix essence and 70 wt% of purified water, UHT heat treatment (sterilization at 135 캜 for 2 seconds) and cooling.

69.5% by weight of the lactic acid bacteria culture solution, 0.1% by weight of Lactobacillus plantarum HY7715 lyophilized powder of Example 1, and 30.4% by weight of the mixed fruit juice syrup were combined and homogenized at 150 bar, A fermented milk containing Lactobacillus plantarum HY7715 of the present invention as an active ingredient was prepared.

≪ Example 3 >

Lactobacillus planta room ( Lactobacillus plantarum ) Preparation of functional beverage containing HY7715 as active ingredient

Mixing juice syrup was 13% by weight of liquid fructose, white sugar, 2.5% by weight, 2.5% by weight brown sugar, mix juice concentrate 56Brix ⁰ 10.9% by weight of the pectin 1.0% by weight, fresh fruit mix Essence 0.1% by weight and pure water 70 wt% 35 ℃ Followed by UHT heat treatment (sterilization at 135 캜 for 2 seconds), followed by cooling. Then, 30.4% by weight of the mixed juice syrup prepared by the above-mentioned method was combined with 0.1% by weight of the lyophobillus plantarum HY7715 of Example 1 and 69.5% by weight of the remaining purified water, homogenized at 150 bar, C, and then packed in a small-sized container such as a glass bottle or PET bottle to prepare a functional beverage containing Lactobacillus plantarum HY7715 as an active ingredient of the present invention.

<Example 4>

Lactobacillus planta room ( Lactobacillus plantarum Production of Health Functional Foods Containing HY7715 as Active Ingredient

B vitamins B ₁ , B ₂ , B ₅ , B ₆ , E and acetic acid ester, nicotinic acid amide) and oligosaccharides were added to 0.1 wt% of Lactobacillus plantarum HY7715 lyophilized powder of Example 1 Was added in an amount of 10 parts by weight based on 100 parts by weight of the Lactobacillus plantarum HY7715 lyophilized powder of Example 1 and mixed in a high-speed rotary mixer. 10 parts by weight of sterilized purified water was added to the mixture, mixed and molded into granules having a diameter of 1 to 2 mm. The molded granules were dried in a vacuum dryer at 50 ° C and then passed through 12 to 14 mesh to uniformly produce granules. The granules thus prepared were extruded in suitable amounts to be purified or powdered or filled into hard capsules to prepare hard capsule products.

&Lt; Example 5 >

Lactobacillus planta room ( Lactobacillus plantarum ) Preparation of cosmetic composition containing HY7715 as an active ingredient

Nutritional lotion

The liquid preparation was prepared by mixing 0.01 wt% of Lactobacillus plantarum HY7715 lyophilized powder of Example 1, 4 wt% of wax, 1.5 wt% of polysorbate 60, 0.5 wt% of sorbitan sesquioleate, 5 wt% of liquid paraffin, 5% by weight squalane, 5% by weight caprylic / capric triglyceride, 3% by weight glycerin, 3% by weight butylene glycol, 3% by weight propylene glycol, 0.1% by weight carboxyvinyl polymer, 0.2% by weight triethanolamine A preservative, a trace pigment, a trace amount of perfume, and a trace amount of purified water were mixed and prepared according to a conventional nutrition lotion production method.

Nourishing cream

, 0.01 wt% of Lactobacillus plantarum HY7715 lyophilized powder of Example 1, 10 wt% of wax, 1.5 wt% of polysorbate 60, 0.5 wt% of sorbitan sesquioleate, 10 wt% of liquid paraffin, 5 parts by weight of squalane, 5 parts by weight of caprylic / capric triglyceride, 5 parts by weight of glycerin, 3 parts by weight of butylene glycol, 3 parts by weight of propylene glycol, 0.2 part by weight of triethanolamine, the remaining preservative, And a trace amount of purified water were mixed and prepared according to a conventional nutrition cream preparation method.

Massage Cream

( Lactobacillus plantarum HY7715 lyophilized powder of Example 1, 0.01 wt% of wax, 10 wt% of wax, 1.5 wt% of polysorbate 60, 0.8 wt% of sorbitan sesquioleate, 40 wt% of liquid paraffin, 5% by weight squalane, 4% by weight caprylic / capric triglyceride, 5% by weight glycerin, 3% by weight butylene glycol, 3% by weight propylene glycol, 0.2% by weight triethanolamine, remaining preservatives, And a trace amount of purified water were mixed to prepare a conventional massage cream.

pack

, 0.01 wt% of Lactobacillus plantarum HY7715 lyophilized powder of Example 1, 13 wt% of polyvinyl alcohol, 0.2 wt% of sodium carboxymethyl cellulose, 0.1 wt% of allantoin, 5 wt% of ethanol, 0.3% by weight, the remaining preservative, trace pigment, trace flavor and trace amount of purified water were mixed and prepared according to a conventional pack preparation method.

&Lt; Test Example 1 >

1-1. Lactobacillus Planta Room (Lactobacillus plantarum) manufacture of raw HY7715 cells

Lactobacillus plantarum HY7715 was inoculated into the MRS liquid medium and cultured at 37 占 폚 for 18 to 20 hours. To confirm the number of viable bacteria, the cultured lactic acid bacterium was subjected to decidual dilution and cultured at 37 ° C. for 2 to 3 days using MRS agar medium to measure the number of bacteria.

When applied to cell experiments, the medium used for cell culture was added based on the previously obtained number of cells, and the cells were diluted and used as live cells.

1-2. Culture of human keratinocyte (HaCaT) cells

Human corneal keratinocytes (HaCaT) and DMEM were subcultured in a 1: 4 weight ratio once every 2-3 days in DMEM medium (Gibco, USA) containing 10% fetal bovine serum and 1% (Culture conditions: 37 ° C incubator supplied with 5% CO ₂ ).

In other words, for the passage, the DMEM medium was removed, washed once with 4 ml of PBS, treated with 1 ml of Trypsin-EDTA Solution 1X (1XTE, Sigma), and placed in the incubator for 10 to 15 minutes. After confirming that the adhesion of keratinocytes (HaCaT) from the human body was deteriorated, 4 ml of DMEM medium was added to recover keratinocytes (HaCaT) from the human body. The recovered human keratinocyte (HaCaT) was centrifuged at 1,200 rpm for 3 minutes, the supernatant was carefully removed, and 1 ml of DMEM medium was added to dissolve the cell pellet. Human keratinocytes (HaCaT) and DMEM medium were diluted in a weight ratio of 1: 4, put into a 100Ø cell culture dish, mixed well and incubated for 2-3 days in an incubator.

1-3. Lactobacillus Planta Room (Lactobacillus plantarum) apoptosis protective effect HY7715

Human keratinocytes (HaCaT) of Test Example 1-2 were inoculated in 96 wells at 2 × 10 ⁴ cells per well and cultured for 24 to 48 hours. Then, DMEM medium without FBS . &Lt; / RTI &gt;

Lactobacillus plantarum HY7715 cells of Test Example 1-1 were dissolved at a concentration of 1 × 10 ⁷ CFU / ml in a DMEM medium not containing FBS, and the cells were washed once with DMEM without FBS (HaCaT) of each well, and at the same time, ERM ^® -CZ100 (European Reference Materials, Sigma), which is a fine dust containing polycyclic aromatic hydrocarbons, was treated at a concentration of 250 μg / Keratinocytes (HaCaT).

Then, the above-mentioned Lactobacillus plantarum HY7715 and microparticles-treated human keratinocytes (HaCaT) were cultured for 20 to 24 hours, and 10 μl of each CCK-8 assay solution was treated , And the cell viability was calculated by measuring the absorbance at 540 nm using a spectrophotometer after 1 to 4 hours.

On the other hand, as the negative control group, the test was carried out in the same manner as above except that the human keratinocyte (HaCaT) was not treated.

As a positive control, the test was carried out in the same manner as above except that the human keratinocyte (HaCaT) was treated with only the fine dust.

Here, 'cell survival rate' means the percentage of the absorbance of the fine dust and the lactic acid bacteria treated after the test divided by the absorbance of the negative control group after the test.

The results are shown in Fig.

As can be seen from FIG. 1, the cell viability was reduced to 68.1% in the positive control group treated with only the fine dust on the basis of the cell viability of 100% of the negative control group, but the microorganism , Lactobacillus plantarum , In the case of HY7715 treated group (HY7715), cell viability increased up to 96.6%. From these facts, it can be seen that Lactobacillus plantarum HY7715 of the present invention has an effect of inhibiting cell death due to fine dust treatment.

&Lt; Test Example 2 &

2-1. Extraction of RNA from human-derived keratinocytes (HaCaT) treated with fine dusts

The human keratinocytes (HaCaT) of Test Example 1-2 were inoculated in 96 wells at 2 × 10 ⁴ cells / well, cultured for 24 to 48 hours, and washed once with DMEM medium containing no FBS.

Lactobacillus plantarum HY7715 cells of Test Example 1-1 were dissolved at a concentration of 1 × 10 ⁷ CFU / ml in a DMEM medium not containing FBS, and the cells were washed once with DMEM without FBS was treated in the human-derived keratinocytes (HaCaT) of each well at the same time the human origin of the fine particles for each well of ^{ERM ® -CZ100 (European Reference Materials,} Sigma) containing the polycyclic aromatic hydrocarbon in a concentration of 50μg / ㎖ Keratinocytes (HaCaT).

Then, the Lactobacillus plantarum HY7715 and microdermabrasion-treated keratinocytes (HaCaT) were cultured for 20 to 24 hours, washed twice with PBS, and then treated with an easy-spin ^™ into a lysis buffer (iNtRON, USA) 1㎖ it was dissolved in the human body-derived keratinocyte (HaCaT). RNA was extracted from the dissociated human keratinocytes (HaCaT) using the easy-spin ^™ [DNA free] Total RNA Extraction Kit (Invitrogen, USA). RNA purity and degree of degradation were confirmed by ND-1000 Spectrophotometer (NanoDrop, Wilmington, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA).

On the other hand, in order to clarify the cause of gene expression change, RNA was extracted in the same manner as above except that only the Lactobacillus plantarum HY7715 cells of Test Example 1-1 were treated.

In order to clarify the cause of gene expression change, RNA was extracted in the same manner as above except that only fine dust was treated as a positive control.

In order to further clarify the cause of the gene expression change, RNA was extracted in the same manner as above except that no negative treatment was carried out on human keratinocytes (HaCaT) as a negative control group.

2-2. Analysis of Gene Expression in Human Dermal Keratinocyte (HaCaT) Treated with Real Time Polymerase Chain Reaction

Lactobacillus plantarum HY7715 was shown to protect against apoptosis. To explain this, we compared CYP1A1 (Cytochrome P450 1A1), which is involved in recognition and toxicity of foreign substances, AhR (Aryl hydrocarbon receptor) gene expression was analyzed.

Namely, cDNAs were synthesized from each of the separated RNAs of Test Example 2-1 except that only Lactobacillus plantarum HY7715 cells were treated with the Omniscript reverse transcription kit (Qiagen, Germany). RNA was expressed and quantified using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, USA) using Taqman gene expression master mix (Taqman, USA). Human primers used for this purpose were ordered through Tagman as GAPDH (Hs03929097_g1), CYP1A1 (Hs01054796_g1) and AHR (Hs00169233_m1). Fold change in gene expression was calculated based on the Ct (treshhold cycle), an index of gene expression.

The results are shown in Fig. 2 (AhR gene expression) and Fig. 3 (CYP1A1 gene expression).

As can be seen from Fig. 2, the gene expression of AhR (Aryl hydrocarbon receptor) in the positive control treated with only fine dust was 5.2 (based on the gene expression 1 of the negative control not treated with keratinocytes (HaCaT) However, the gene expression of AhR (Aryl hydrocarbon receptor) decreased 2.3 times in the experimental group (HY7715) treated with micro dust and Lactobacillus plantarum HY7715 cells simultaneously.

As can be seen from FIG. 3, gene expression 1 of the negative control group treated with nothing in human keratinocyte (HaCaT) was compared with that of CYP1A1 (Cytochrome P450 1A1) gene in the positive control group treated with only fine dust, However, the expression of CYP1A1 (Cytochrome P450 1A1) decreased to 11.1 fold in the experimental group (HY7715) treated with micro dust and Lactobacillus plantarum HY7715 cells simultaneously.

2 and FIG. 3, the Lactobacillus plantarum HY7715 of the present invention contains an AhR (Aryl hydrocarbon receptor) and CYP1A1 (Cytochrome P450 1A1 ) Gene expression, it was confirmed that it has a protective effect against fine dust.

Korea Biotechnology Research Institute KCTC13101BP 20160908

Claims (8)

delete Lactobacillus plantarum HY7715 (accession number: KCTC 13101BP), which has the effect of preventing the skin damage due to fine dust by reducing the expression of AhR (Aryl hydrocarbon receptor) gene in human skin cells, As an active ingredient to prevent damage to the skin caused by fine dust.
Lactobacillus plantarum HY7715 (accession number: KCTC 13101BP), which has the effect of preventing and improving damage of skin caused by fine dusts by decreasing the expression of CYP1A1 (Cytochrome P450 1A1) gene in human skin cells, As an active ingredient to prevent damage to the skin caused by fine dust.
The method according to claim 2 or 3,
Wherein the food composition has one of the formulations selected from the group consisting of fermented milk, health functional food, and functional beverage.
delete Lactobacillus plantarum HY7715 (accession number: KCTC 13101BP), which has the effect of preventing the skin damage due to fine dust by reducing the expression of AhR (Aryl hydrocarbon receptor) gene in human skin cells, As an active ingredient, to prevent or reduce damage to the skin caused by fine dust.
Lactobacillus plantarum HY7715 (accession number: KCTC 13101BP), which has the effect of preventing and improving damage of skin caused by fine dusts by decreasing the expression of CYP1A1 (Cytochrome P450 1A1) gene in human skin cells, As an active ingredient, to prevent or reduce damage to the skin caused by fine dust.
The method according to claim 6 or 7,
Wherein the cosmetic composition has one of the formulations selected from a nutritional lotion, a nutritional cream, a massage cream, and a pack.

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