KR101972925B1 - Cosmetic composition for skin irritation alleviation with 2'-fucosyllactose - Google Patents

Abstract

The present invention provides a cosmetic composition containing 2′-fucosyllactose. The 2′-fucosyllactose of the present invention provides an effect of reducing transepidermal water loss and alleviating skin redness, thereby exhibiting an effect of moisturizing and soothing the skin (effect of alleviating irritation) whose barrier is damaged by external stimuli.

Description

[0002] Cosmetic composition for skin irritation alleviation with 2'-fucosyllactose containing 2'-fucosyllactose [

The present invention relates to a cosmetic composition for relieving skin irritation containing 2'-fucosyllactose.

Skin, the largest organ of the human body, has various physiological functions such as a function to protect various internal organs of the skin from the outside, a barrier function to prevent the internal moisture and oil components from leaking out, temperature control function, excretion function, It is a very important institution in charge. In general, the skin is divided into three parts: upper layer of skin, epidermis, dermis, subcutaneous fat layer.

A person's skin experiences various skin troubles due to environmental changes, stress, and the like. In the case of infancy, skin problems such as atopic dermatitis are experienced. During adolescence, the skin becomes dry, elasticity is weakened and wrinkles are formed as the aging process progresses. It is also susceptible to weak external stimuli and takes a long time to recover. Especially, recently, various kinds of cosmetics have been used to cause various troubles on the skin, and researches utilizing natural ingredients as a representative method for solving such skin troubles have been actively conducted.

On the other hand, fucosyllactose, which is an oligosaccharide derived from human breast milk, has recently attracted attention as a material for infant formula, health functional foods for the elderly, and pharmaceuticals. Accordingly, the present invention provides a cosmetic composition containing 2'-fucosyllactose.

Korean Patent Publication No. 10-2018-0051688 (published on Aug. 17, 2018) discloses a cosmetic-related composition containing 2'-fucosyllactose or 3-fucosyllactose. Korean Patent No. 10-1731263 (registered on Apr. 24, 2014) describes a method for producing 2'-fucosyllactose using Corynebacterium glutamicum.

The present invention is to provide a cosmetic composition for skin irritation mitigation or skin moisturization containing 2'-fucosyllactose.

The present invention provides a cosmetic composition for relieving skin irritation containing 2'-fucosyllactose.

The present invention also provides a cosmetic composition for skin moisturizing, characterized by containing 2'-fucosyllactose.

In the cosmetic composition of the present invention, the 2'-fucosyllactose is preferably added in an amount of 0.000001 to 50 wt% of the total cosmetic composition.

In the cosmetic composition of the present invention, the cosmetic composition may be a solution, suspension, emulsion, paste, lotion, gel, water-soluble liquid, cream, essence, cleansing oil containing oil, oil / (W / O) type and a combination thereof; skin; Lotion; Eye cream; Soothing gel; Ointment; A formulation for a mask pack; A body-wasting formulation; Peeling gel; Underwater type and wet type makeup base; foundation; Skin cover; Lipstick, lip gloss, face powder, two-way cake, eye syrup, cheek color, and eyebrow pencil; Formulation for scalp; Or the like.

The cosmetic composition of the present invention contains 2'-fucosyllactose derived from milk, thereby exhibiting skin irritation alleviation and skin moisturizing effects. In addition, the cosmetic composition according to the present invention may be applied to various formulations and used as a cosmetic composition showing skin irritation mitigating effect or skin moisturizing effect.

FIG. 1 is a graph showing an average value of the transdermal water loss (TEWL) measured in Experimental Example 1. FIG.
FIG. 2 is a graph showing the averaged value of skin redness measured in Experimental Example 1. FIG.

The present invention provides a cosmetic composition for relieving skin irritation, which comprises 2'-fucosyllactose.

Human breast milk contains more than 200 unique human oligosaccharides (HMO) at significantly higher concentrations (5-15 g / L) than other mammalian milk. HMOs provide a variety of biological activities, such as prebiotic effects that help the growth of intestinal lactic acid bacteria, prevention of pathogen infection, immune system regulation, and brain development, which have positive effects on infant development and health.

HMO can be produced by a combination of D-glucose (Glc), D-galactose (Gal), N-acetylglucosamine, GlcNAc, L-fucose, Fuc and sialic acid [Sia; N-acetyl neuraminic acid (Neu5Ac)]. Because the structure of the HMO is so diverse and complex, there can be as many as 200 isomers with different residues and glycosyl bonds in different degrees of polymerization (DP 3-20). However, despite its structural complexity, HMOs have several common structures. Most HMOs have a lactose (Galβ1-4Glc) residue at the reducing end. Gal of lactose is formed by α- (2,3) - and α- (2,6) -bonds in the form of 3-sialyllactose or 6-sialyllactose, respectively, Or 2'-fucosyllactose, 2'-FL or 3-fucosyllactose, respectively, as α- (1,2) - and α- (1,3) , 3-FL). ≪ / RTI >

Approximately 200 other complex oligosaccharides were found in breast milk, 137 of which were fucosylated, including three of the most abundant oligosaccharides, accounting for almost 77% of the total oligosaccharides. The remaining oligosaccharides were mostly sialylated (39) %. In particular, 2'-fucosyllactose and 3-fucosyllactose are reported to be major HMOs involved in the various biological activities mentioned above. In recent years, fucosyllactose has attracted attention as a material for infant formula, health-functional foods for the elderly, and medicines. In the present invention, it is a composition for alleviating skin irritation or a skin moisturizing cosmetic composition comprising 2'-fucosyllactose .

In the cosmetic composition for alleviating skin irritation or skin moisturizing, which contains 2'-fucosyllactose of the present invention, the 2'-fucosyllactose may be added in an amount of 0.000001 to 50 wt% of the total cosmetic composition .

On the other hand, the cosmetic composition of the present invention can be used as a cosmetic composition, for example, as a solution, suspension, emulsion, paste, lotion, gel, water-soluble liquid, cream, essence, cleansing oil containing surfactant, oil, (W / O) type; skin; Lotion; Eye cream; Soothing gel; Ointment; A formulation for a mask pack; A body-wasting formulation; Peeling gel; Underwater type and wet type makeup base; foundation; Skin cover; Lipstick, lip gloss, face powder, two-way cake, eye syrup, cheek color, and eyebrow pencil; Formulation for scalp; Or the like.

In addition, the cosmetic composition of the present invention may contain adjuvants commonly used in cosmetics such as hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, perfumes, fillers, blockers, pigments, absorbers, dyes and the like. The amount of these various adjuvants is an amount commonly used in the art, for example, 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the adjuvant and its proportion will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

When the formulation is a paste, a cream or a gel in the cosmetic composition of the present invention, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, Talc or zinc oxide may be used.

In the cosmetic composition of the present invention, when the formulation is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, Benzoate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.

In the cosmetic composition of the present invention, when the formulation is a suspension, water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester , Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like may be used.

On the other hand, in the cosmetic composition of the present invention, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component when the formulation is a powder or a spray, Propellants such as chlorofluorohydrocarbons, propane / butane or dimethyl ether.

On the other hand, in the case of the cosmetic composition of the present invention, when the formulation is a surfactant-containing cleansing agent, aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, sarcosinates, Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

Further, in the cosmetic composition of the present invention, when the composition is a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation or soap, it may be applied to the skin and then wiped off or washed off with water. The surfactant-free cleansing formulation may be a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, A cleansing lotion, a cleansing water and a cleansing gel, but is not limited thereto.

In addition, the cosmetic composition of the present invention can be used in combination with other cosmetic compositions other than the present invention. The cosmetic composition according to the present invention may be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

On the other hand, in the cosmetic composition of the present invention, the cosmetic composition may be used for relieving skin irritation, strengthening skin barrier, alleviating skin flushing, or for skin cleansing. It may also be for moisturizing the skin.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following embodiments and experimental examples, and includes modifications of equivalent technical ideas.

[Example 1: Preparation of cosmetic composition containing 2'-fucosyllactose]

In this Example, a cosmetic composition containing 2'-fucosyllactose was formulated at a blending ratio as shown in Table 1 below.

ingredient Content (% by weight) 2'-fucosyllactose 0.2 EDTA-2Na 0.02 Purified water to 100 Cetostearyl alcohol 2.0 Glyceryl stearate 1.5 Microcrystalline 0.7 Squalane 5.0 Liquid paraffin 3.0 Trioctanoin 5.0 Polysorbate 1.2 Sorbitan stearate 0.5 Tocopheryl acetate 0.2 Cyclomethicone 3.0 BHT 0.05 Incense, preservative Suitable amount

[Experimental Example 1: Evaluation of skin irritation mitigation effect of 2'-fucosyllactose]

In this Experimental Example, the skin irritation mitigating effect of the cream containing 2'-fucosyllactose prepared in Example 1 was evaluated.

All topical application agents, including cosmetics, are potentially irritating substances that can cause skin irritation or dryness on short or long term use. Therefore, most topical application agents include ingredients that can calm the skin irritation reaction and reduce the dryness, such as glycerol, tarurine, glycine and the like. Tests to see the sedative effect of topical application or skin irritation of certain ingredients often involve artificial induction of stimuli, where SLS (Sodium Lauryl Sulfate) is used as a stimulus inducing ingredient. SLS is often used in skin irritation prevention and restoration tests of moisturizers and barrier strengtheners, and it can cause erythema and sometimes infiltration or superficial erosion when applied to the skin for a certain period of time.

A variety of methods have been used to measure the sedative effects of skin irritation reactions, the most sensitive and accurate method known to measure TEWL (Trans-Epidermal Water Loss). According to the literature, TEWL is known to continuously increase for 2 or 3 days after patch removal when applying 1 ~ 2% SLS for 24 hours patch. In case of application of external preparation with sedative effect of skin irritation during this period It is known that the decrease of the increase width of TEWL is reduced and it is used in the evaluation test of the skin stimulation reaction so as to objectively measure the skin irritation reaction and its soothing effect using the TEWL measurement.

This study was carried out to examine the effects of 2'-fucosyllactose on the skin elasticity and skin redness in 21 healthy adult women aged 20 to 65 years without skin disease. The degree of decrease was measured and compared with the control group (non - embryo).

The transdermal water loss (TEWL) was measured with a Tewameter TM-300 (Corage + Khazaka, Germany). Tewameter is an open chamber type measuring device that measures the percutaneous water loss per unit time. In the case of skin barrier damage, the measured value is increased.

The skin redness (a-value) was measured with a Chromameter CR-400 (Minolta, Japan). Chromameter is a device that converts the color of the surface into digital codes of L, a, and b using two wavelengths of reflected light. L is brightness, a is green to red spectrum, and b is blue to yellow spectrum .

All of the measurements in this experiment are shown as the average of 5 measurements.

(1) skin irritation

1% SLS (Sodium Lauryl Sulfate, GFC, Korea) is an anionic surfactant that causes skin irritation and causes erythema and occasionally invasion and epidermal erosion. The test group was divided into two test groups at a distance of 5 cm or more from the wrist of one of the forearms of the subject. The test group was cleaned using a standard detergent, followed by tapping with a paper towel to remove moisture, followed by 30 minutes of constant temperature and humidity (20 to 24 ° C, 60% RH). TEWL ₀ and a ₀ -value were measured in each test group, and 15 μl of 1% SLS was applied for 24 hours using an 8 mm finn chamber.

(2) 2'-fucosyllactose primary pap

After 24 hours of 1% SLS patch application, the patches were removed and rinsed with water. TEWL ₁ and a ₁ -value of each test group were measured after stabilization at constant temperature and humidity condition (20 to 24 ° C, 40 to 60% RH) for 30 minutes. Then, 15 [mu] l of 2 ' -fructosyllactose-containing cream and a non-dermal test group were randomly assigned to two test groups and patches were applied for 24 hours. The cream containing 2'-fucosyllactose was applied with 15 μl of sample using an 8 mm finn chamber.

(3) Measurement of Primary Patch Results and 2'-Fucosyllactose Secondary Patch

The 2'-fucosyllactose-containing cream primary patch patch was applied for 24 hours, removed, and rinsed with water. TEWL ₂ and a ₂ -value of each test group were measured after stabilization at constant temperature and humidity condition (20 to 24 ° C, 40 to 60% RH) for 30 minutes. Then, 2'-fucosyllactose-containing cream and a non-wrinkle test group were placed in the same manner as in the primary patch test group, and patches were applied for 24 hours. The cream containing 2'-fucosyllactose was applied with 15 μl of sample using an 8 mm finn chamber.

(4) Secondary Patch Results Measurement

The 2'-fucosyllactose-containing cream secondary patch patch was applied for 24 hours, then removed, and washed with water. TEWL ₃ and a ₃ -value of each test group were measured after stabilization at constant temperature and humidity condition (20 to 24 ° C, 40 to 60% RH) for 30 minutes.

The TEWL values measured above are shown in Table 2.

The TEWL value (g / m ² h) measured in Experimental Example 1 division Test group Control group Subject Before processing Skin irritation 24 hours
process 48 hours
process TEWL ₀ TEWL ₁ TEWL ₂ TEWL ₃ TEWL ₀ TEWL ₁ TEWL ₂ TEWL ₃ One 8.24 12.43 10.40 9.50 9.82 11.69 12.75 14.51 2 24.69 24.46 20.26 12.29 24.37 24.18 23.65 24.45 3 16.54 34.32 12.98 13.27 20.02 37.04 18.07 17.11 4 7.44 18.86 15.79 7.01 13.49 18.94 17.79 26.30 5 9.46 13.71 9.78 7.96 8.97 14.85 10.86 13.21 6 9.36 15.55 12.27 9.59 9.22 14.41 14.11 12.53 7 13.27 14.96 10.96 10.42 13.41 15.70 24.55 21.75 8 12.15 19.85 14.26 17.50 10.25 20.62 24.45 25.63 9 9.98 16.42 13.80 10.05 10.68 16.10 16.24 17.01 10 12.90 21.73 14.89 9.65 11.11 20.53 22.38 17.49 11 8.43 19.07 16.61 6.75 10.49 19.52 19.51 21.62 12 18.18 32.41 16.61 6.75 11.46 18.83 48.09 25.33 13 7.70 10.55 9.90 11.45 7.73 12.38 12.30 18.11 14 12.26 22.55 21.11 7.80 14.89 20.25 21.65 14.47 15 10.07 17.29 16.97 17.79 8.44 17.24 24.72 30.65 16 8.72 13.47 11.39 9.82 9.83 11.60 13.26 17.41 17 9.38 20.50 18.75 6.22 8.99 22.35 21.65 20.18 18 15.18 18.72 10.54 6.52 8.41 18.65 10.72 9.44 19 21.80 11.01 10.04 7.98 23.17 10.80 12.76 9.32 20 12.84 28.98 18.79 11.45 14.05 26.55 28.01 18.11 21 11.00 27.35 22.84 18.09 9.36 28.59 33.65 30.80

The average values of the TEWL measurement values in Table 2 are shown in Table 3 below.

(G / m ² h) of the TEWL values measured in Experimental Example 1, TEWL ₀ TEWL ₁ TEWL ₂ TEWL ₃ Control group 12.29 19.09 20.53 19.31 Test group 12.36 19.72 14.71 10.37

The values in Table 3 are shown graphically in FIG. As shown in Table 3, the TEWL values measured after 24-hour treatment (TEWL ₂ ) and 2'-fucosyllactose-containing cream treatment (TEWL ₃ ) of 2'-fucosyllactose-containing cream according to the present invention decreased Respectively. On the other hand, in the control group, TEWL value increased after skin irritation (TEWL ₁ ).

Accordingly, it was confirmed that the test group to which the 2'-fucosyllactose-containing cream of the present invention was applied showed less water loss than the control group, and it was confirmed that the skin moisturizing effect of 2'-fucosyllactose-containing cream was excellent.

On the other hand, the a-values measured in the above are shown in Table 4.

The a-value measured in Experimental Example 1 division Test group Control group Subject Before processing Skin irritation 24 hours
process 48 hours
process a ₀ -value a ₁ -value a ₂ -value a ₃ -value a ₀ -value a ₁ -value a ₂ -value a ₃ -value One 5.89 7.23 7.48 7.08 6.48 7.87 7.88 7.86 2 4.22 7.36 6.45 6.17 6.33 6.81 6.37 6.73 3 4.85 6.84 7.98 7.77 5.17 8.42 8.17 8.20 4 5.55 8.76 10.64 9.50 5.62 7.58 9.39 10.25 5 6.37 6.90 6.53 7.03 5.06 7.72 6.49 7.66 6 3.93 5.36 5.98 6.06 4.59 6.13 6.25 6.78 7 6.67 6.47 6.33 6.51 4.76 6.35 7.33 8.86 8 7.16 8.62 7.99 8.09 6.28 8.59 9.70 9.82 9 5.78 9.36 7.57 7.73 5.37 8.17 8.55 8.54 10 6.00 7.73 9.06 6.64 6.99 10.30 8.78 8.91 11 6.95 9.07 7.36 7.33 8.23 10.66 10.39 10.44 12 5.46 7.29 6.54 6.36 5.88 9.28 8.77 8.85 13 5.48 6.56 5.94 5.87 3.99 5.21 5.89 5.94 14 5.52 5.73 7.75 7.27 5.19 6.35 6.88 6.80 15 6.05 8.78 8.01 7.37 4.82 7.24 7.34 7.33 16 4.43 5.97 6.25 6.39 3.97 7.47 6.72 6.58 17 3.65 6.35 5.20 5.94 5.04 8.18 7.90 8.67 18 7.45 7.90 7.54 7.44 7.16 7.22 8.56 7.99 19 5.38 10.83 5.74 5.83 3.99 9.47 5.72 5.98 20 4.89 10.15 10.93 11.16 7.23 10.55 9.61 10.39 21 7.62 8.28 8.45 8.64 5.69 8.97 8.86 8.91

The average values of the measured values shown in Table 4 are shown in Table 5 below.

The average value of the a-value measured in Experimental Example 1 a ₀ -value a ₁ -value a ₂ -value a ₃ -value Control group 5.61 8.03 7.88 8.17 Test group 5.68 7.69 7.42 7.25

The values in Table 5 above are shown graphically in FIG. As shown in Table 5, the 2'-fucosyllactose-containing cream according to the present invention was measured in a ₂ -value after 24-hour treatment and in a ₃ -value after 2'-fucosyllactose-containing cream treatment And skin redness was alleviated. On the other hand, skin irritation was not alleviated from the a ₁ -value after skin irritation in the control group.

Accordingly, it was confirmed that the test group to which the 2'-fucosyllactose-containing cream of the present invention was applied alleviated skin irritation as compared with the control group, and it was confirmed that the skin-soothing effect of 2'-fucosyllactose-containing cream was excellent.

Taken together, the cosmetic composition containing 2'-fucosyllactose showed an effect of significantly reducing the transdermal water loss of the skin, and showed an effect of alleviating the redness of the skin.

Therefore, it has been confirmed that the cosmetic composition containing 2'-fucosyllactose of the present invention has skin moisturizing effect and skin soothing effect on external stimuli.

Claims (4)

0.2% by weight of 2'-fucosyllactose, 0.02% by weight of ethylenediamine-tetraacetic acid disodium salt, 77.63% by weight of purified water, 2.0% by weight of cetostearyl alcohol, A mixture of 1.5% by weight of Glyceryl stearate, 0.7% by weight of Microcrystalline, 5.0% by weight of Squalan, 3.0% by weight of Liquid paraffin, 5.0% by weight of Trioctanoin, 1.2% by weight of Polysorbate, 0.5% by weight of Sorbitan stearate, 0.2% by weight of Tocopheryl acetate, 3.0% by weight of Cyclomethicone and 0.05% by weight of BHT (Dibutyl hydroxy toluene) Wherein the cosmetic composition contains at least one member selected from the group consisting of water,
delete delete The method according to claim 1,
In the cosmetic composition,
(O / W) type and water-in-oil type (W / O) type, which are selected from the group consisting of solutions, suspensions, emulsions, pastes, lotions, gels, water-soluble liquids, creams, essences, Basic cosmetic formulation; skin; Lotion; Eye cream; Soothing gel; A formulation for a mask pack; A body-wasting formulation; Peeling gel; Underwater type and wet type makeup base; foundation; Skin cover; Lipstick, lip gloss, face powder, two-way cake, eye syrup, cheek color, and eyebrow pencil; Formulation for scalp; And the cosmetic composition is a cosmetic composition.

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