KR101967809B1 - Composition for anti-wrinkle and moisturizing skin Comprising Nano-Sized Lactic Acid Bacteria from Kimchi - Google Patents

Abstract

The invention of wrinkle or skin moisturizing relates to a cosmetic composition, and more particularly to skin wrinkles or skin moisturizing the skin aging improved effectiveness superior Lactobacillus Planta room (L. plantarum) with lactic acid bacteria for using the Kimchi lactic acid bacteria To a cosmetic composition using nano-sized dead cells.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for improving skin wrinkles or skin moisturizing cosmetics containing nano-sized Kimchi lactic acid bacteria.

The invention of wrinkle or skin moisturizing relates to a cosmetic composition, and more particularly to skin wrinkles or skin moisturizing the skin aging improved effectiveness superior Lactobacillus Planta room (L. plantarum) with lactic acid bacteria for using the Kimchi lactic acid bacteria To a cosmetic composition using nano-sized dead cells.

Kimchi is a Korean traditional food fermented by lactic acid bacteria. The lactic acid bacteria classified in kimchi include Leuconostoc mesenteroides, Leuconostoc dextranicum, Lactobacillus brevis, Lactobacillus plantarum, Pediococcus pentosacus, and the like.

Lactic acid bacteria (lactobacillus) is a bacterium that breaks down saccharides such as glucose to produce lactic acid. It is also called lactic acid bacteria. It has the property of inhibiting growth of pathogenic bacteria and harmful bacteria by lactic acid produced by lactic acid fermentation. It is used for food production such as brewed food. In addition, lactic acid bacteria live in the intestines of mammals to prevent abnormal fermentation by germs and are also used as enteric agents. The above-mentioned lactic acid bacteria are gram-positive bacteria, and have various properties such as lactic acid, anaerobic or anaerobic, and Lactobacillus and Streptococcus.

Patents on lactic acid bacteria isolated from kimchi include lactic acid bacteria Lactococcus lactis BH5 (Korean patent No. 413335), Lactobacillus plantarum NC (Korean patent No. 771209), which has excellent antioxidative activity, (Korean Patent No. 1068531) and the like.

However, conventionally disclosed Kimchi lactic acid bacteria are all related to probiotics, and strains capable of growing in a certain volume of culture medium are limited.

On the other hand, sunlight consists of visible light, ultraviolet light, and infrared light. Among them, ultraviolet light synthesizes vitamin D in the body and plays a role of sterilizing action, but it causes dermatitis, skin cancer, sunburn, skin aging, Also. Ultraviolet (UV) is divided into three kinds according to wavelength, A, B and C, among which UVC is blocked in the ozone layer and UVA and UVB affect the skin.

Skin aging can be divided into photo aging and natural aging, and the main cause of photo aging is repetitive ultraviolet exposure. Particularly, UVB (290 ~ 320nm), which is the middle wavelength among ultraviolet rays, can cause serious skin damage when exposed to acute or chronic, and it can break collagen, elastin, hyaluronic acid, There is a problem in inducing loss and consequently producing thick wrinkles.

Korean Patent No. 10-413335 Korean Patent No. 10-771209 Korean Patent No. 10-1068531

Accordingly, it is an object of the present invention to provide a cosmetic composition having excellent skin wrinkle-reducing effect by using nano-sized dead cells of lactic acid bacteria isolated from kimchi, and to provide a cosmetic composition having skin moisturizing effect by using nanosized dead cells of lactic acid bacteria isolated from kimchi It is another object to provide such an excellent cosmetic composition.

The present invention also provides a cosmetic composition capable of improving skin wrinkles or improving skin aging through skin moisturizing effect using nano-sized dead cells of lactic acid bacteria isolated from kimchi.

And, the present invention containing the nano Kimchi lactic acid bacteria use the cells as an active ingredient, wherein the nano Kimchi lactic acid bacteria is Lactobacillus Planta room (Lactobacillusplantarum) nF1 strain (accession number NITE P-1462) In order to achieve the above object, the The present invention provides a cosmetic composition for improving skin wrinkles, wherein the nanoized Kimchi lactic acid bacterium cells are in the form of a strain.

The nanoized Kimchi lactic acid bacterium cells can be obtained by culturing Lactobacillus plantarum nF1 strain (Accession No. NITE P-1462) at a pH of 5 to 6; Heat sterilization of the culture broth of the strain at 60 ° C to 70 ° C for 8 to 10 minutes; Adding dextrin in an amount equal to the weight of the cells to the culture solution, and dispersing the mixture in a high pressure homogenizer at 140 to 160 kgf / cm ² ; And lyophilizing and pulverizing the dispersed culture liquid.

In addition, the nanoized Kimchi lactic acid bacteria cells may have a mode particle size distribution mode of 0.5 to 1.0 占 퐉.

In addition, the nanoized Kimchi lactic acid bacteria may contain 100 to 5 trillion Lactobacillus plantarum nF1 strains per 1 g of the lyophilized powder.

The cosmetic composition may be at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, a skin softener, a skin toner, A lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, a foundation, a nutrition essence, a sunscreen, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser .

In accordance with still another aspect of the present invention, the present invention containing the nano Kimchi lactic acid bacteria use the cells as an active ingredient, wherein the nano Kimchi lactic acid bacteria is Lactobacillus Planta room (Lactobacillusplantarum) nF1 strain (accession number NITE P-1462 ), And the nanoized Kimchi lactic acid bacterium cells are in the form of a bacterium.

The nanoized Kimchi lactic acid bacterium cells can be obtained by culturing Lactobacillus plantarum nF1 strain (Accession No. NITE P-1462) at a pH of 5 to 6; Heat sterilization of the culture broth of the strain at 60 ° C to 70 ° C for 8 to 10 minutes; Adding dextrin in an amount equal to the weight of the cells to the culture solution, and dispersing the mixture in a high pressure homogenizer at 140 to 160 kgf / cm ² ; And lyophilizing and pulverizing the dispersed culture liquid.

In addition, the nanoized Kimchi lactic acid bacteria cells may have a mode particle size distribution mode of 0.5 to 1.0 占 퐉.

In addition, the nanoized Kimchi lactic acid bacteria may contain 100 to 5 trillion Lactobacillus plantarum nF1 strains per 1 g of the lyophilized powder.

The cosmetic composition may be at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, a skin softener, a skin toner, A lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritional cream, a moisturizing cream, a hand cream, a foundation, a nutrition essence, a sunscreen, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser .

The cosmetic composition for improving skin wrinkles or skin moisturizing comprising the nano-sized kimchi lactic acid bacterium cells as an active ingredient according to the present invention suppresses wrinkling of the skin and increases the moisture content of the skin to improve the aging of the skin have.

The cosmetic composition according to the present invention is advantageous in that the nanoized Kimchi lactic acid bacterium cells are in the form of a bacterium, that is, a spherical form, whereby the coagulation phenomenon of the cosmetic composition can be suppressed and the absorption rate can be improved. There is an advantage that it is possible to provide a cosmetic composition having skin wrinkle improvement or skin moisturizing effect superior to the case where the form of the cells is in the form of a bacterium.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is an image of a colony observation image (a) and a result of Gram stain (a) of the Kimchi lactic acid bacteria of the present invention.
FIG. 2 is a molecular system diagram showing the 16S rRNA sequence of Kimchi lactic acid bacteria (SIID11558) of the present invention analyzed (the lower left line indicates the scale bar, the number at the branch of the plant is the bootstrap value, (Type strain).
Fig. 3 is a micrograph of the Kimchi lactic acid bacteria of the present invention observed before and after the nano-treatment. (a) before nano-treatment: after sticking, (b) after nano-treatment: spherical shape.
Figure 4 relates to an experimental model and ultraviolet radiation dose of the present invention.
FIG. 5 shows the result of measuring the weight change of the experimental model of the present invention.
Figure 6 shows the results of observing the degree of skin damage observed by ultraviolet exposure of the experimental model of the present invention.
Fig. 7 shows the results of observing the wrinkle formation inhibitory effect of the experimental model of the present invention.
Figure 8 shows the results of observing the skin moisture content of the experimental model of the present invention.

Hereinafter, the present invention will be described in detail.

One aspect of the invention, and containing the nano Kimchi lactic acid bacteria use the cells as an active ingredient, wherein the nano Kimchi lactic acid bacteria is Lactobacillus Planta room (Lactobacillusplantarum) nF1 strain (accession number NITE P-1462), and wherein the nano the Kimchi Wherein the lactic acid bacterium cells are in the form of a bacterium.

Another aspect of the invention, and containing the nano Kimchi lactic acid bacteria use the cells as an active ingredient, wherein the nano Kimchi lactic acid bacteria is Lactobacillus Planta room (Lactobacillusplantarum) nF1 strain (accession number NITE P-1462), and wherein the nano the Kimchi Wherein the lactic acid bacterium cells are in the form of a bacterium.

The present inventors isolated lactic acid bacteria from kimchi and found out the most excellent microorganisms for improving skin wrinkles and skin moisturizing effects to improve skin aging. As a result, they were found to be dead cells of Lactobacillus plantarum . The Lactobacillus planta strain was deposited on Nov. 8, 2012 as "nF1" in the National Institute of Technology and Evaluation (NITE) (NITE P-1462).

It is preferable that the nanoized Kimchi lactic acid bacterium cells according to the present invention are in the form of a bacterium. In general, lactic acid bacteria are known to undergo changes in shape due to the stress when the growth environment during culture is not constant or poor. Thus, in the present invention, the lactic acid bacteria are proliferated so that the form of the strain of the lactic acid bacterium is kept constant by controlling the culture and processing conditions to be constant. In other words, the lactic acid bacteria form is in the form of a bacterium (rod shape) before the nano-treatment, but the lactic acid bacteria form (spherical shape) through the nano-processing can be suppressed and the absorption rate can be improved.

The nano-sized Kimchi lactic acid bacterium according to the present invention comprises a step of culturing Lactobacillus plantarum nF1 strain (Accession No. NITE P-1462) under the condition of pH 5 to 6; Heat sterilization of the culture broth of the strain at 60 ° C to 70 ° C for 8 to 10 minutes; Adding dextrin in an amount equal to the weight of the cells to the culture solution, and dispersing the mixture in a high pressure homogenizer at 140 to 160 kgf / cm ² ; And lyophilizing and pulverizing the dispersed culture liquid.

In one embodiment of the present invention, the nanoized Kimchi Lactobacillus cells are heat killed for 8 to 10 minutes at 60 ° C to 70 ° C for quenching. In the case of live cells, there is a possibility of causing a morphological change at the time of delivery or display after manufacture of the product, and therefore, it is preferable that the microorganism does not cause abnormal morphological change.

The nanoized Kimchi lactic acid bacterium of the present invention is subjected to a dispersion treatment step. For the dispersion treatment, the cultured medium treated with the microorganism is dispersed in a wet type using a high-pressure homogenizer at 150 kgf / cm ² (1.5 MPa). In this case, it is preferable to previously add a known dispersant or excipient to the culture liquid, thereby effectively preventing the re-aggregation of the cells. Suitable dispersants and excipients include dextrins, trehalose, dextrin, skim milk, and the like.

In order to finally obtain the nanoized Kimchi lactic acid bacteria of the present invention as a powder, it is preferable to add a known dispersant, excipient or the like to the culture to disperse the cells so as not to cause re-aggregation, and then freeze-dry. Thus, a cell powder having excellent dispersibility in water can be obtained.

It is preferable that the mode particle size distribution of the nano-sized Kimchi lactic acid bacteria of the present invention is 0.5 to 1.0 mu m. The " mode in particle size distribution " refers to a particle diameter at which the relative frequency in the particle size distribution when the particle size of the cells is measured is an index indicating the size of the bacteria. The nano-sized Kimchi lactic acid bacterium of the present invention has been finely granulated to a nanometer (nm) size with a particle size of 1.0 m or less. When the mode of the particle size distribution of the nanoized Kimchi lactic acid bacteria cells satisfies the above range, the skin wrinkle can be further improved or the skin moisturizing effect can be obtained.

The nanoized Kimchi lactic acid bacterium cells may contain 100 to 5 trillion Lactobacillus plantarum nF1 strains per 1 g of the lyophilized powder.

The concentration of Kimchi lactic acid bacteria Lactobacillus plantarum strain can be controlled per 1 g of dry powder according to the amount of the dispersant or excipient added when preparing the nanoized kimchi lactic acid bacteria of the present invention. The present invention is characterized in that the Kimchi lactic acid bacterium strain is contained in an amount of 100 to 5 trillion per 1 g of the nano-sized lactic acid bacterium powder.

In the cosmetic composition according to an embodiment of the present invention, the cosmetic composition may be at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, Lotion, Skin Softener, Skin Toner, Astringent, Lotion, Milk Lotion, Moisture Lotion, Nutrition Lotion, Massage Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Nutrition Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Creams, body lotions, and body cleansers. However, the present invention is not limited thereto. The cosmetic composition comprising each of these formulations may contain various bases and additives necessary for formulation of the formulation, and the kinds and amounts of these ingredients can be easily selected by those skilled in the art.

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal fiber, a plant fiber, a wax, a paraffin, a starch, a tragacanth, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Etc. may be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane-butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the cosmetic composition of the present invention is an interfacial-active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acethionate, imidazolinium derivative, methyltaurate, sarcosinate , Fatty acid amide ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolenic derivative or ethoxylated glycerol fatty acid ester.

The cosmetic composition of the present invention may further contain an excipient including a fluorescent substance, a fungicide, a hygroscopic substance, a moisturizer, a fragrance, a fragrance carrier, a protein, a solubilizer, a sugar derivative, a sunscreen, a vitamin, .

Hereinafter, the present invention will be described in more detail with reference to Examples.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. will be.

Example  1. Identification of Novel Kimchi Lactic Acid Bacteria

For the development of novel Kimchi lactic acid bacteria, the present inventors diluted the kimchi extract and cultured in MRS agar (Oxoid, Hampshire, UK) at 30 ° C under aerobic conditions for 48 hours. After the cultivation, milky white colonies with shine in the form of a typical lactic acid bacterium were selected and separated, and the selected colonies were separated by pure culture in a third culture medium. The strain (SIID11558), which has the best effect of improving the intestinal environment among pure cultured lactic acid bacteria, was finally selected and analyzed.

As shown in Fig. 1, the SIID11558 strain forms a milky white colony, and is morphologically Gram-positive and has a bacterium size of 0.8 ~ 0.9 × 1.2 ~ 1.5 ㎛.

Technos Co. Lu wrap (TechnoSuruga Laboratory Co., Ltd.) 16S rRNA of the strains isolated from the gene sequence were performed for determination and analysis, a strain isolated by this, Lactobacillus Planta room (Lactobacillusplantarum) and 99.9% of the high And was identified as a novel microorganism having the same sex. The isolated 16S rRNA base sequence of the isolated strain is shown in SEQ ID NO: 1, and the phylogenetics tree showing the molecular systematic position is shown in FIG. The primer (5'-ga gtt tga tcc tgg ctc ag-3 ': SEQ ID NO: 2) and the reverse primer (5'-tcg taa caa ggt agc c-3': SEQ ID NO: 3) were used for PCR amplification of 16S rDNA The operation from the PCR amplification to the cycle sequence was based on the following protocols.

- DNA Extraction: Aromolytic DNAase (Wako Junya, Osaka)

- PCR: PrimeSTAR HS DNA Polymerase (Takara Bio, Shiga)

- Cycle Sequencing: BigDye Terminator v3.1 Cycle Sequencing Kit (Life Technologies, CA, USA)

Sequencing: ABI PRISM 3130 x1 Genetic Analyzer System (Life Technologies, CA, USA)

- Sequencing: Chromas Pro 1.5 (Technelysium Pty Ltd., Tewantin, AUS)

- BLAST homology search and simple molecular system analysis: Apollo 2.0 software (Technos Lugarab, Shizuoka)

- Database: Apollo DB-BA 7.0 database (Technosuruga Lab, Shizuoka), International base sequence database (GeneBank / DDBJ / EMBL)

The new microorganism of the present invention identified by the above method is described in the International Patent Organism Depositary (IPOD), National Institute of Technology and Evaluation (NITE) (NITE P-1462). ≪ / RTI >

Example  2. Nanoized  Kimchi lactic acid bacteria nF1 Dead cells  Preparation of powder

The nF1 strain isolated and identified in Example 1 was cultured in a known nutrient medium supplemented with 5% by mass glucose and cultured in a 20 medium-water-sodium hydroxide aqueous solution, pH neutral and 36.5 ° C, and glucose Was used as the culture end point. The pH of the nano-sized Kimchi lactic acid bacteria powder of the present invention is preferably in the neutral range of pH 5 to 7, and pH 6.5 is the best.

After completion of the culture, the culture was sterilized by heating at 80 DEG C for 10 minutes, and then the cells were washed with PBS. Dextrin, 9 times the weight of the cells, was added as an excipient and dispersed with a mixer. Next, it was frozen spray dried to prepare a powder sample. It was confirmed that more than 100 million pieces of Kimchi lactic acid bacteria nF1 strain of the present invention were contained per 1 g of the powder.

The nF1 powder was suspended in PBS while adjusting to a cell concentration of 10 mg / ml. The pH at the processing step was maintained at 6.5. As shown in FIG. 3, the nF1 strain extracted from kimchi was a bar-shaped bacterium before the nano-treatment. However, after the nano-treatment, the nF- It can be understood that they have a property of not re-aggregating. The nano - treated nF1 strain showed a mode particle size distribution of 0.5 ~ 1.0 ㎛, and it has a non - re - agglutinating property, so that lactic acid bacteria having excellent dispersibility in water or other solvents can be obtained.

On the other hand, when dextrin in an amount equivalent to the weight of the cells was added as an excipient, it was confirmed that 5 liters of Kimchi lactic acid bacteria were contained per 1 g of the powder.

Example  3. Skin aging improvement effect experiment

(1) Experimental model and ultraviolet irradiation

Six-week-old female hairless mice (SKH-1) were purchased from Central Korea (Seoul, Korea) and used for 1 week. SKH-1 mice are thinner than nude mice and are closest to human skin. (I) control group (normal), (ii) UVB-irradiated group (UVB), (iii) UVB-irradiated and nF1 0.2 g group, (iv) UVB-irradiated group and nF1 2 g group v) UVB-irradiated and nF1 2 g (No serum; acetone) groups. UVB irradiation was investigated with four Westeting hous F520 lamps (National Biological, Twinsburg, OH). A UVB irradiation chamber equipped with a Kodak Kodacel K6808 filter was created to remove wavelengths below 290 nm (UVC). Ultraviolet light was irradiated 3 times a week (Mon, Wed, Fri) in the dorsal part of the hairless mice. Ultraviolet irradiation dose was 1 MED (1 MED = 0.5 J / ㎠, MED; minimal erythema dose) 3 MED at 3 weeks, and 4 MED at 4 to 12 weeks. This can be confirmed schematically through FIG.

(2) Sample processing

One hour before irradiation with ultraviolet light, 100 μL of a sample was applied to a hairless mouse or the like (25 × 55 mm). The normal group was not treated, the UVB group treated only with serum, and the experimental group treated with serum mixed with nF1 at each concentration. The no serum group was treated with nF1 and acetone.

The serum used in the sample was prepared according to a conventional emulsion preparation method. Specifically, (i) the serum was applied only in the case of the control group (normal), (ii) in the case of the UVB-irradiated group (UVB) (Iv) UVB-irradiated and nF1 2 g group was applied 2 g serum and nF1, (v) UVB-irradiated For the nF1 2 g (No serum; acetone) group, acetone was used instead of serum, and 2 g of acetone and nF1 were applied.

(3) Weight measurement

Body weights were measured once a week during the test period for all animals used in the test. In particular, weighing during the test period was measured using the same scales at the same time of day on the same day to minimize the error in each measurement.

(4) skin wrinkle measurement

After 12 weeks of UV irradiation, the skin of the hairless mouse was simulated with silicone rubber to measure the degree of wrinkle formation. After shameless mice were anesthetized, silicone polymer (Silfo, Flexico Developments LDT, England) reagent was mixed and spread thinly on the dorsal part of the hairless mouse, completely dried and then carefully removed from the replica, The tail part was checked to make a simulated plate. The produced simulated plates were analyzed by Oriental Industry Support Center (Daegu, Korea). Skin wrinkle curves were evaluated through the replica using a skin wrinkle measuring device (VL650, Courage-khazaka Electronic) and the characteristics of various skin parameters were calculated as a parallel light source and a b / w CMOS camera.

(5) Measurement of skin moisture content

(Skin-O-Mat, Cosmomed, Germany), which measures the skin moisture content of hairless mice after skin irradiation for 12 weeks, using skin static capacitance measurement. The skin-O-Mat probe (100-240 VAC 1.4 A) was well calibrated to prevent movement, and the dorsal skin of dulled mice was measured in a room with constant temperature and humidity conditions.

(6) Statistical processing

All experimental results are shown using mean and standard deviation or standard error. Duncan's multiple range test was used for comparison between the groups. Statistical significance was determined when the p value was less than 5% compared with the control group.

Experimental Example

(1) skin damage observed by weight change and ultraviolet exposure

Body weights were measured once a week during the test period for all animals used in the test. No animals died during the test period in all groups, including the normal group, and it was determined that weight loss was caused by stress due to UVB irradiation as the intensity of UVB irradiation increased from 4 weeks later. However, the groups treated with nF1 did not show a significant change in weight compared to the UVB group. The specific weight change in the skin aging test model by UVB can be confirmed through FIG.

In addition, skin damage observed by ultraviolet exposure is caused by vasodilation and sunburn, skin erythema, pain and pruritus, and erythema may be caused by an increased inflammation reaction due to ultraviolet irradiation and an increase in blood flow due to the release of histamine And redness was observed in mice irradiated with ultraviolet rays for 10 weeks compared to the normal group. This can be confirmed from FIG.

(2) Results of skin wrinkle observation

Repeated exposure to ultraviolet light increases matrix metalloproteinases (MMPs) that degrade substrate proteolysis, such as collagen fibers, elastic fibers, fibronectin, and laminin, and destroys collagen fibers that give the skin elasticity and strength, And the effect of nF1 on mouse skin wrinkles caused by UVB irradiation was evaluated because it formed coarse and deep wrinkles as compared with endogenous aging. The area of wrinkles produced by repeated UVB irradiation was significantly increased compared to the normal group. The concentration of the nFl - treated group was significantly decreased in comparison with that of the UVB group. Similar effects were also observed in the nF1 group mixed with acetone. This effect can be specifically confirmed with reference to FIG. Fig. 7A shows the results of inhibition of nF1 by wrinkles caused by ultraviolet rays, and B is a value obtained by quantifying the results.

(3) Skin moisture content measurement result

UVB damage the skin's dermis and as a result, the barrier of the skin collapses and the balance of oil and water in the skin is unbalanced, which causes damage to the skin. Therefore, the moisture content of the skin, which is a skin moisturizing marker, is analyzed . As a result, the skin moisture content was decreased by UVB irradiation, and the moisture content of the nF1-treated mouse group was significantly increased by about 20% as compared with the UVB irradiation group. This effect can be specifically confirmed with reference to FIG. FIG. 8 is a value obtained by quantifying the effect of nF1 on the skin moisture content by ultraviolet rays.

(4) Sintering

In the skin aging experiment model induced by UVB, it was confirmed that nF1 effectively improves the wrinkles caused by skin aging and maintains moisture of the skin. As a result, it was confirmed that nF1 had an excellent effect on skin aging.

Independent administrative corporation Product evaluation Technology-based organization Patented microorganism deposit center nF1NITEP-1462 20121108

<110> BIOGENICS KOREA CO., LTD <120> Nano-Sized Lactic Acid Bacteria from Kimchi <130> 13P1169 <160> 3 <170> Kopatentin 2.0 <210> 1 <211> 1492 <212> DNA <213> Artificial Sequence <220> <223> 16S rRNA of Lactobacillus plantarum gene: nF1 <400> 1 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gaactctggt attgattggt 60 gcttgcatca tgatttacat ttgagtgagt ggcgaactgg tgagtaacac gtgggaaacc 120 tgcccagaag cgggggataa cacctggaaa cagatgctaa taccgcataa caacttggac 180 cgcatggtcc gagcttgaaa gatggcttcg gctatcactt ttggatggtc ccgcggcgta 240 ttagctagat ggtggggtaa cggctcacca tggcaatgat acgtagccga cctgagaggg 300 taatcggcca cattgggact gagacacggc ccaaactcct acgggaggca gcagtaggga 360 atcttccaca atggacgaaa gtctgatgga gcaacgccgc gtgagtgaag aagggtttcg 420 gctcgtaaaa ctctgttgtt aaagaagaac atatctgaga gtaactgttc aggtattgac 480 ggtatttaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 540 gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggttttt taagtctgat 600 gtgaaagcct tcggctcaac cgaagaagtg catcggaaac tgggaaactt gagtgcagaa 660 gaggacagtg gaactccatg tgtagcggtg aaatgcgtag atatatggaa gaacaccagt 720 ggcgaaggcg gctgtctggt ctgtaactga cgctgaggct cgaaagtatg ggtagcaaac 780 aggattagat accctggtag tccataccgt aaacgatgaa tgctaagtgt tggagggttt 840 ccgcccttca gtgctgcagc taacgcatta agcattccgc ctggggagta cggccgcaag 900 gctgaaactc aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc 960 gaagctacgc gaagaacctt accaggtctt gacatactat gcaaatctaa gagattagac 1020 gttcccttcg gggacatgga tacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga 1080 tgttgggtta agtcccgcaa cgagcgcaac ccttattatc agttgccagc attaagttgg 1140 gcactctggt gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc 1200 atgcccctta tgacctgggc tacacacgtg ctacaatgga tggtacaacg agttgcgaac 1260 tcgcgagagt aagctaatct cttaaagcca ttctcagttc ggattgtagg ctgcaactcg 1320 cctacatgaa gtcggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc 1380 cgggccttgt acacaccgcc cgtcacacca tgagagtttg taacacccaa agtcggtggg 1440 gtaacctttt aggaaccagc cgcctaaggt gggacagatg attagggtga ag 1492 <210> 2 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> forward primer <400> 2 gagtttgatc ctggctcag 19 <210> 3 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 3 tcgtaacaag gtagcc 16

Claims (10)

And containing the nano Kimchi lactic acid bacteria use the cells as an active ingredient, wherein the nano Kimchi lactic acid bacteria is Lactobacillus Planta room (Lactobacillusplantarum) nF1 strain (accession number NITE P-1462), and wherein the nano Kimchi lactic acid bacteria use the cells are bacteria form a ,
The nanoized Kimchi lactic acid bacterium cells can be obtained by culturing Lactobacillus plantarum nF1 strain (Accession No. NITE P-1462) at a pH of 5 to 6;
Heat sterilization of the culture broth of the strain at 60 ° C to 70 ° C for 8 to 10 minutes;
Adding dextrin in an amount equal to the weight of the cells to the culture solution, and dispersing the mixture in a high pressure homogenizer at 140 to 160 kgf / cm ² ; And
The present invention relates to a cosmetic composition for improving skin wrinkles, which comprises the steps of lyophilizing and drying a dispersed culture liquid.
delete The method according to claim 1,
Wherein the nanoized Kimchi lactic acid bacterium cells have a mode particle size distribution mode of 0.5 to 1.0 占 퐉.
The method according to claim 1,
The cosmetic composition for improving skin wrinkles, wherein the nanoized Kimchi lactic acid bacterium cells contain about 1 to 5 trillion Lactobacillus plantarum nF1 strains per 1 g of the lyophilized powder.
The method according to claim 1,
The cosmetic composition may be at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, a skin softener, a skin toner, A lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a foundation, a nutrition essence, a sunscreen, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser Wherein the composition is a single formulation.
And containing the nano Kimchi lactic acid bacteria use the cells as an active ingredient, wherein the nano Kimchi lactic acid bacteria is Lactobacillus Planta room (Lactobacillusplantarum) nF1 strain (accession number NITE P-1462), and wherein the nano Kimchi lactic acid bacteria use the cells are bacteria form a ,
The nanoized Kimchi lactic acid bacterium cells can be obtained by culturing Lactobacillus plantarum nF1 strain (Accession No. NITE P-1462) at a pH of 5 to 6;
Heat sterilization of the culture broth of the strain at 60 ° C to 70 ° C for 8 to 10 minutes;
Adding dextrin in an amount equal to the weight of the cells to the culture solution, and dispersing the mixture in a high pressure homogenizer at 140 to 160 kgf / cm ² ; And
And lyophilizing and drying the dispersed culture liquid. [Claim 7] The cosmetic composition for skin moisturizing according to claim 1,
delete The method according to claim 6,
Wherein the nanoized Kimchi lactic acid bacterium cells have a mode particle size distribution mode of 0.5 to 1.0 占 퐉.
The method according to claim 6,
The cosmetic composition for skin moisturizing according to claim 1 , wherein the nano-sized Kimchi lactic acid bacterium cells contain 100 to 5 trillion Lactobacillus plantarum nF1 strains per 1 g of the lyophilized powder.
The method according to claim 6,
The cosmetic composition may be at least one selected from the group consisting of an ointment for external use, a cream, a softening agent, a nutritional lotion, a pack, an essence, a hair tonic, a shampoo, a rinse, a hair conditioner, a hair treatment, a gel, a skin lotion, a skin softener, a skin toner, A lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutrition cream, a moisturizing cream, a hand cream, a foundation, a nutrition essence, a sunscreen, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion and a body cleanser Wherein the composition is a single formulation.

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