KR102173646B1 - Streptococcus salivarius sbk4 strain isolated from skin and functional cosmetic composition comprising Streptococcus salivarius sbk4 strain - Google Patents

Abstract

The present invention relates to a new strain of Streptococcus salivarius sbk4 and a cosmetic composition using the new strain. Streptococcus salivarius sbk4 strain isolated and identified from the skin according to the present invention (accession number: KCTC13872BP), a culture, cell lysate or extract thereof, contains a large amount of phenol and flavonoids, and has excellent superoxide radical or ABTS radical scavenging ability. Since it contains, it can be used not only in the manufacture of functional cosmetics having an antioxidant function, but also has excellent skin barrier improvement activity, so it can be easily used in the manufacture of functional cosmetics for improving skin barrier and strengthening skin barrier.

Description

[Streptococcus salivarius sbk4 strain isolated from skin and functional cosmetic composition comprising Streptococcus salivarius sbk4 strain}

The present invention relates to a skin-derived new strain, Streptococcus salivarius sbk4, and a functional cosmetic composition using the same.

As the economic level of modern people improves and participation in social life increases, interest in skin beauty and health is increasing to a high level in various age groups. In accordance with the purpose of preventing skin aging and suppressing skin stress, preventing skin aging and maintaining clean and healthy skin, many beauty-related cosmetics and foods have been developed.

Free radicals or free radicals are formed by various biological reactions such as normal metabolic processes by xanthine oxidase or glutathione reductase in mitochondria, phagocytic cells or cytoplasm, or inflammatory reactions by ultraviolet rays or external stimuli. do. If such highly reactive reactive oxygen species and free radicals, especially superoxide anion radicals, which are very reactive and destructive, accumulate in excess in the human body, in addition to inflammatory reactions, cell aging, stroke, myocardial infarction, diabetic vascular disorders , Hyperlipidemia, diabetes, neurodegenerative diseases, etc. will cause various human diseases. Therefore, it is reported that the scavenging material capable of removing the active oxygen or free radicals has an effect of preventing or treating these diseases, and studies to develop various active oxygen (or free radical) scavenging materials from microorganisms and plants have been conducted. It continues.

In addition, the development of functional cosmetics that have the function of suppressing the production of free radicals or free radicals is also continuing. Due to the low preference of consumers for synthetic compounds, recently developed products are being developed using natural products as materials. .

On the other hand, lactic acid bacteria are bacteria that play a beneficial role in the human body and produce lactic acid (lactic acid) using sugars such as carbohydrates. So far, more than 400 species have been discovered and are also applied to the manufacture of cosmetics. The fermentation broth obtained by culturing these lactic acid bacteria in a medium mainly composed of skim milk, whey, sugars, etc. is used for skin softning by increasing the rate of turnover of the stratum corneum. ), it has been reported to be effective in relieving skin wrinkles, etc., but in reality, these functional effects of cosmetics containing these fermented broths are most often expressed through other ingredients added to cosmetics rather than through active ingredients in the fermented broth.

However, as of yet, as a functional cosmetic for skin improvement and skin health, there has not been an example developed using lactic acid bacteria as the main ingredient.

Accordingly, the present inventors completed the present invention by confirming that a functional cosmetic having excellent antioxidant activity and skin barrier improving ability can be manufactured using strains derived from the human body and human skin.

Korean Patent Registration 10-1788544

Accordingly, an object of the present invention is to provide a Streptococcus salivarius sbk4 strain (accession number: KCTC13872BP) isolated from the skin.

Another object of the present invention is to provide a cosmetic composition having antioxidant activity, comprising the strain of Streptococcus salivarius sbk4 (accession number: KCTC13872BP), the culture of the strain, the lysate of the strain or the extract of the strain as an active ingredient Is to do.

Another object of the present invention is Streptococcus salivarius sbk4 strain (accession number: KCTC13872BP), a culture of the strain, a lysate of the strain or an extract of the strain as an active ingredient, for improving or strengthening skin barriers It is to provide a cosmetic composition.

In order to achieve the object of the present invention as described above, the present invention provides a Streptococcus salivarius sbk4 strain (accession number: KCTC13872BP) isolated from the skin.

In one embodiment of the present invention, the strain may have antioxidant activity, anti-inflammatory activity, and skin barrier improvement activity.

In addition, the present invention provides a cosmetic composition having antioxidant activity, comprising the strain of Streptococcus salivarius sbk4 (accession number: KCTC13872BP), the culture of the strain, the lysate of the strain or the extract of the strain as an active ingredient.

In one embodiment of the present invention, the cosmetic composition may have a superoxide radical scavenging ability, an ABTS radical scavenging ability, or a nitrogen monoxide scavenging ability.

In addition, the present invention provides a cosmetic composition for improving or strengthening skin barriers, comprising the strain of Streptococcus salivarius sbk4 (accession number: KCTC13872BP), the culture of the strain, the lysate of the strain or the extract of the strain as an active ingredient. to provide.

In one embodiment of the present invention, the cosmetic composition may promote or enhance the formation of a cornified envelope.

In one embodiment of the present invention, the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence , Pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder, and loose powder. have.

Streptococcus salivarius sbk4 strain isolated and identified from the skin according to the present invention (accession number: KCTC13872BP), a culture, cell lysate or extract thereof, contains a large amount of phenol and flavonoids, and has excellent superoxide radical or ABTS radical scavenging ability. Since it contains, it can be used not only in the manufacture of functional cosmetics having an antioxidant function, but also has excellent skin barrier improvement activity, so it can be easily used in the manufacture of functional cosmetics for improving skin barrier and strengthening skin barrier.

1 is a schematic diagram of a schematic diagram of Streptococcus salivarius sbk4 isolated and identified from human skin in an embodiment of the present invention.
Figure 2 shows the results of analyzing the superoxide radical scavenging ability according to the lysate (SSC) treatment of the streptococcus salivarius sbk4 new strain of the present invention in skin keratinocytes.
Figure 3 shows the results of analyzing the nitrogen monoxide scavenging ability in skin keratinocytes according to the lysate (SSC) treatment of the streptococcus salivarius sbk4 new strain of the present invention.
Figure 4 shows the results of analysis of the ability to promote the formation of a cornified envelope in skin keratinocytes according to treatment with the lysate (SSC) of the new strain of Streptococcus salivarian sbk4 of the present invention.

The present invention is characterized by providing a Streptococcus salivarian sbk4 strain isolated and identified from human skin and a functional cosmetic composition using the strain.

The inventors of the present invention isolated a new strain belonging to Streptococcus salivarius from human skin while researching to identify a new lactic acid bacterium that has lactic acid bacteria itself as a major component and can be a raw material for manufacturing functional cosmetics with excellent antioxidant activity. , This strain was named Streptococcus salivarian sbk4 strain, and by confirming its excellent antioxidant activity, it was confirmed that the strain can be used in the manufacture of antioxidant cosmetics.

Specifically, according to an embodiment of the present invention, the present inventors isolated a new microorganism present in the skin from a human skin sample, analyzed the 16s rRNA sequence of the identified strains, streptococcus, a new strain that has not been previously revealed. A strain of the genus Salivarius was identified, and it was named Streptococcus salivarius sbk4 strain, and was deposited with the Korea Research Institute of Bioscience and Biotechnology on June 26, 2019, and was given the deposit number KCTC13872BP.

Further, in the present invention, as a result of studying the physiological activity of the identified Streptococcus salivarius sbk4 strain, it was confirmed that it has excellent scavenging ability against ABTS radicals and superoxide radicals.

Therefore, the present inventors found that the strain of Streptococcus salivarius sbk4 of the present invention can be usefully used in the manufacture of cosmetics having antioxidant activity.

Accordingly, the present invention can provide a cosmetic composition comprising a Streptococcus salivarius sbk4 strain, a culture thereof, a lysate thereof, or an extract thereof as an active ingredient.

The cosmetic composition of the present invention has antioxidant activity, and in particular, has excellent scavenging ability against superoxide radicals or ABTS radicals.

In addition, in an embodiment of the present invention, after treating the lysate of the Streptococcus salivarian sbk4 strain of the present invention on HaCaT cells, which are keratinocytes, the degree of formation of the cornified envelope is analyzed to affect the skin barrier. The impact was analyzed.

As a result, the group treated with the lysate of the Streptococcus salivarius sbk4 strain of the present invention showed an increase in the formation of the cornified envelope compared to the untreated group, and the strain of the present invention has the effect of improving the skin barrier. I could see that there is this.

The skin is a representative organ that exhibits an immune response while retaining moisture in the body and performing an essential barrier function to protect the internal invasion of external invading factors. The most important role of the skin barrier is to protect our body, which is composed of about 80% water, from the dry external environment, and this barrier function is created and maintained in the epidermis, the outer layer of the skin. The epidermal layer, including the skin barrier, is one of the most dynamic organs in the human body and maintains epidermal homeostasis by constantly repeating the processes of formation, differentiation, and shelling of epidermal cells.

This skin barrier function appears through the stratum corneum structure consisting of keratinocytes, cornified envelope, lamellar membrane lipid, intercorneocyte lipid, and corneodesmosome. Proteins related to the skin barrier As a component, the envelope protein is well known.

Therefore, when the above factors involved in the skin barrier function are not formed or there is an abnormality, the skin barrier is weakened and various skin diseases may occur.

In this respect, the strain of the present invention improved the skin barrier as it was confirmed that the lysate of the strain of the present invention, specifically the sbk4 strain, has an activity capable of promoting the formation and proliferation of the cornified envelope (CE) in skin keratinocytes. Or it could be seen that it could be strengthened.

In the present invention, the cultivation refers to all actions performed to grow microorganisms under appropriately artificially controlled environmental conditions. In addition, the culture includes not only the live bacteria obtained from the culture medium, but also any processing form for lactic acid bacteria known to those skilled in the art, and includes, for example, lysed cells, dried products, frozen products, and the like, but is not limited thereto.

Furthermore, the ingredients contained in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the Streptococcus salivarian sbk4 strain, a culture product thereof, a lysate or an extract thereof as an active ingredient, such as an antioxidant, And conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments and fragrances, and carriers.

The cosmetic composition of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation, spray, etc. may be formulated, but is not limited thereto. More specifically, the cosmetic composition of the present invention may be prepared in the form of a flexible lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can. When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan. When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used. When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether. When the formulation of the present invention is a surfactant-containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

When the cosmetic composition of the present invention is a soap, a cleansing formulation containing a surfactant, or a cleansing formulation containing no surfactant, it may be applied to the skin and then wiped off, removed, or washed with water. As a specific example, the soap is liquid soap, powder soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, and the cleansing formulation without surfactant is a cleansing cream , Cleansing lotion, cleansing water, and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through examples. These examples are for explaining the present invention more specifically, and the scope of the present invention is not limited to these examples.

<Example 1>

Isolation and identification of lactic acid bacteria derived from skin

<1-1> Sample collection and lactic acid bacteria separation

The skin surface microbes were swapped in the cheek part of an 11-year-old boy. A Quick Swab kit (3M Microbiology, USA) was used for the swab, and the swab method was collected based on a surface area of about 12 cm ² of the test subject based on the method recommended by the swab kit manufacturer. Each of the swapped samples was inoculated by streak smearing using flame sterilized platinum on solid EM medium containing agar, and incubated under aerobic conditions at 37°C for 3 days until single colonies were identified, and then a single colony, an independent colony. The strain was subcultured three times by streak smearing on a new plate to increase the growth activity of the strain, and the nucleotide sequence of the isolated strain was analyzed by separating single colonies cultured in the above process.

<1-2> nucleotide sequence analysis

In the process of <1-1>, nucleotide sequence analysis of 16S rRNA was performed on the purely isolated strain. For nucleotide sequence analysis, genomic DNA was isolated using a chromosomal DNA extraction kit, and PCR was performed from the obtained gDNA using universal primers to analyze nucleotide sequence. The homology test for the results obtained by performing 16s rRNA sequencing was performed through the BLAST search database provided by NCBI.

As a result of nucleotide sequence analysis, it was found that the lactic acid bacterium isolated in the present invention is a strain belonging to the genus Streptococcus salivarius, and as a result of nucleotide sequence analysis of 16s rRNA, the strain of the present invention was confirmed to be a new strain that was not previously present. They named the isolated and identified strain as Streptococcus salivarius sbk4 strain, and it was deposited with the Korea Research Institute of Bioscience and Biotechnology on June 26, 2019, and was given the deposit number KCTC13872BP.

In addition, the nucleotide sequence of the 16s rRNA of Streptococcus salivarian sbk4 isolated and identified in the present invention is shown in SEQ ID NO: 1.

<Example 2>

Antioxidant Activity Analysis of Streptococcus salivarius sbk4 Strain

<2-1> Preparation of a culture of Streptococcus salivarius sbk4

The lactic acid bacteria Streptococcus salivarius sbk4 strain isolated in the present invention was inoculated into MRS broth medium, cultured at 37° C. for 48 hours under aerobic conditions, and then the culture medium was centrifuged (3,000 g, 10 minutes) to recover the pellet, This was washed 3 times with PBS and resuspended in PBS again. The cell suspension washed three times was crushed with ultrasonic waves on an ice bath for 10 minutes, centrifuged at 10,000 g for 10 minutes to remove cell debris, recovered only the supernatant, and then filtered through a membrane filter (0.45 μm) to the present invention. A cell lysate, which is a culture of the Streptococcus salivarian sbk4 strain, was obtained.

<2-2> Analysis of total polyphenol content

After taking 10 μL of the cell lysate (sample) of the strain of the present invention obtained in <2-1>, 90 μL of distilled water was added, 10 μL of 1 N Folin-Ciocalteu reagent was added and reacted at room temperature for 5 minutes. After adding 100 μL of a 7% Na ₂ CO ₃ solution and reacting for 10 minutes, the absorbance was measured at 750 nm using a spectrophotometer. The total phenol content was calculated by dissolving gallic acid in distilled water, measuring the absorbance in the same way as above, and substituting it into the prepared standard calibration curve.

<2-3> Analysis of total flavonoid content

25 μL of 1 N NaOH was added to 25 μL of the cell lysate (sample) of the strain of the present invention obtained in <2-1> above, and after mixing, 250 μL of diethylene glycol was added and reacted at room temperature for 1 hour, using a spectrophotometer. The absorbance was measured at 420 nm. A standard curve was prepared using naringin as a standard reagent, and the total flavonoid content was expressed as mg naringin equivalent.

As a result of the analysis, it was found that the culture of the strain of the present invention contained large amounts of total phenol and total flavonoids.

<2-3> ABTS scavenging ability measurement

The present inventors confirmed that the strain culture of the present invention, particularly the cell lysate of the strain, contains a large amount of phenol and flavonoids through the experiments of the above <2-1> and <2-2>. To confirm, the ABTS radical scavenging ability was analyzed. For this, 7.4 mM ABTS and 2.6 mM potassium persulfate were reacted in the dark for 12 hours. Per well of a 96-well plate, 10 μL of the cell lysate of the strain according to the present invention was added, and 290 μL of the diluted ABTS solution was added to react for 30 minutes, and then absorbance was measured at 750 nm. The degree of decrease in absorbance as much as radical scavenging by the sample was measured. At this time, ascorbic acid, known to have an antioxidant effect, was used as a positive control for activity comparison.

As a result, it was found that Streptococcus salivarius sbk4, the strain according to the present invention, has ABTS radical scavenging ability, and its activity is more excellent than ascorbic acid used as a positive control.

<2-4> Superoxide radical scavenging ability measurement

Furthermore, the present inventors performed the following experiment to confirm whether the superoxide radical scavenging activity for the sbk4 strain of the present invention. To this end, HaCaT cells were cultured in a 6-well plate under the condition of 2 × 10 ⁵ cells/well for 24 hours, and then TNF-α (10 ng/mL) was pretreated for 2 hours, and then the lysate of the sbk4 strain of the present invention After treating 20 μL and 80 μL of the sample (ssc) for 24 hours each, after taking the cell culture supernatant, the remaining cells were washed twice in 1X HBSS buffer 1 mL/well condition, and then 100 μM DCFH-DA solution dissolved in 1X HBSS buffer Treated with 500 μL/well. After the reaction in a 37°C CO ₂ incubator for about 30 minutes, the mixture was washed twice with 1 mL/well of 1X HBSS buffer, and fixed with 4% formaldehyde solution at 4° C. for 2 hours or more. Then, the 4% formaldehyde solution was removed by washing twice in 1X HBSS buffer 1 mL/well condition, and finally, cells were obtained under 800 μL/well condition of 1X HBSS buffer, and 200 μL each was loaded into a 96 well plate and a luminometer. Measured at 527 nm.

As a result, as shown in FIG. 2, it was found that the superoxide radical increased by treating the HaCaT cell line with TNF-α was significantly reduced when 80 μL of the strain lysate sample (ssc) of the present invention was treated. Through these results, it was confirmed that the cell lysate of the sbk4 strain of the present invention has a scavenging ability of superoxide radicals that cause damage to cells and tissues, and the strain lysate of the present invention has protective efficacy against photooxidative stress in skin tissues. I could see that I could have.

<Example 3>

Analysis of anti-inflammatory activity of Streptococcus salivarius sbk4 strain

In order to confirm the anti-inflammatory activity against the Streptococcus salivarius sbk4 strain of the present invention, the scavenging ability of nitrogen monoxide was analyzed. Nitric oxide (NO) is a mediator that induces inflammation in vivo, and is known to be produced in the process of decomposing L-arginine into L-citrulline by NO synthase (NOS). Therefore, the scavenging ability of nitrite oxide of perfumery material plays an important role as a functional cosmetic to relieve dermatitis. In addition, when inflammatory reactions are induced in skin tissues due to external harmful stimuli and damage, keratinocytes (HaCaT) are stimulated by secreting TNF-α, an inflammatory cytokine that causes inflammation from macrophages. oxide It is known to worsen the inflammation of the skin tissue. Accordingly, the present inventors confirmed through the following experiment whether the strain of the present invention has anti-inflammatory activity against nitric oxide-mediated inflammatory reaction induced by TNF-α stimulation.

To this end, the HaCaT cell line was cultured for 24 hours in a 6-well plate under 2 × 10 ⁵ cells/well conditions, and then TNF-α (10 ng/mL) was pretreated for 2 hours, and the strain lysate of the present invention (ssc ) 20 μL and 80 μL each were treated for 24 hours, and then 500 μL of the culture supernatant was taken to measure nitrite. 50 μL of Griess reagent was added to 50 μL of the HaCaT culture supernatant, and after reacting for 10 minutes at room temperature, absorbance was measured at 540 nm using a spectrophotometer. The total amount of nitrite was calculated by substituting the obtained standard curve after measuring the absorbance using NaNO ₂ (64 μM ~ 0 μM) solution.

As a result, as shown in Figure 3, the nitrite increased by treating the HaCaT cell line with TNF-α was found to decrease the amount of nitrite by the treatment of the strain lysate (ssc) of the present invention, and this effect is a positive control. Ascorbic acid (ascobic acid; AA) 1mM treatment showed almost the same level. Through these results, the present inventors found that the sbk4 strain of the present invention has excellent anti-inflammatory activity.

<Example 4>

Analysis of skin barrier improvement and strengthening activity of Streptococcus salivarius sbk4 strain

Skin barrier function has become an important target in the development of potential cosmetics. When the skin barrier function is lowered, negative effects such as moisturizing, anti-inflammatory, and microbiota pattern change appear, and in particular, it is a cause of skin inflammatory diseases such as atopic inflammation. Accordingly, the present inventors performed the following experiment to confirm whether the strain lysate of the present invention has the activity of improving the skin barrier.

HaCaT cells, keratinocytes, were cultured in Dulbecco' modified eagle medium (DMEM) medium supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin. Cells were cultured in a 6-well plate under the condition of 6 × 10 ⁵ cells/well for 24 hours, and after replacement with FBS-free medium, 20 μL and 80 μL of cell lysate samples (ssc) of the sbk4 strain were treated every 2 days. Incubated for about 5 days. After washing the cells with PBS, add 1 mL of a solution containing 2% Sodium dodecyl Sulfate (SDS) and 20 mM Dithiothreitol (DTT), stir for 3 minutes, heat at 95 °C for 20 minutes, and extract the cornified envelope (CE). , The amount of the cross-linked envelope was analyzed by measuring absorbance at 310 nm.

As a result, as shown in FIG. 4, the formation of cornified envelope (CE) was increased in the group treated with the strain lysate of the present invention compared to the group not treated with the strain lysate sample (ssc) of the present invention. Therefore, through these results, the present inventors found that the strain of the present invention has an activity to promote the formation of a cornified envelope (CE), which is one of the components constituting the skin barrier, and thus has an activity to improve and strengthen the skin barrier.

<Example 5>

Preparation of cosmetics containing Streptococcus salivarius sbk4 strain

<5-1> Toner manufacturing

The toner containing the crushed product of the Streptococcus salivarius sbk4 strain (Accession No.: KCTC13872BP) obtained in the above example was mixed with each added amount of the ingredients listed in the table below, and the same method as in conventional toner production was used. Thus, a functional cosmetic toner was prepared. At this time, the oil phase and the aqueous phase were weighed, heated to 80°C, mixed to induce emulsification, and as the content of the strain lysate of the present invention increased, the color became darker and a little peculiar smell was produced. There was no significant difference, especially, since no change in the formulation was observed, the content of the strain lysate of the present invention was determined to be a maximum of 5%.

Toner production ingredient Toner prescription (%) Lysis solution of Streptococcus salivarius sbk4 strain of the present invention 5 Carbomer 0.7 Butylene Glycol 2.5 glycerin 4 Diglycerin 0.5 Niacinamide 4 PEG-40 Hydrogenates Castor Oil One Tridesses-9 0.6 Expert Gel EG56 4 Macadamia Nut Oil 0.5 Cetyl alcohol 0.5 Hydrogenated Lecithin 0.1 Shea Butter 0.1 Oleth-10 0.1 Spices a very small amount Purified water Remaining amount (to be 100)

<5-2> Cream production

The toner containing the lysate of Streptococcus salivarius sbk4 strain of the present invention was mixed with each added amount of the ingredients listed in the following table to prepare a cream as a functional cosmetic using the same method as in conventional cream preparation.

Cream production ingredient Cream prescription (%) Lysis solution of Streptococcus salivarius sbk4 strain of the present invention 5 Carbomer One Butylene Glycol 2.5 glycerin 4 Diglycerin 0.3 Hexanediol 2 Niacinamide 2 PEG-40 Hydrogenates Castor Oil One Hydroxypropylgua 2 Polysorbate 80 2 Dipropylene glycol 2 Glyceryl stearate 2 Cetylethylhexanoate One Macadamia seed oil One Polyglyceryl-3 methyl glucose
Distearate One Cetearyl alcohol One Triethylhexanoin 0.5 Dimethicone 0.5 Shea Butter 0.5 Diglycerin 0.5 Carbomer 0.3 Oleth-10 0.3 Cetyl alcohol 0.1 Hydrolyzed Collagen 0.5 Spices a very small amount Purified water Remaining amount (to be 100)

< Example 6>

Acceptance evaluation

A sample of a cream and essence product containing the strain of the present invention was provided to 20 female panelists in their 20s to 50s, and after being allowed to use for 2 weeks, preference was investigated on a 5-point scale for each item.

As a result of the evaluation, as shown in Table 3 above, both toner and cream cosmetics manufactured using the strain of the present invention were generally found to have excellent acceptability. In particular, considering the average score, the toner received the highest evaluation for absorbency, and the cream product received the highest evaluation for the portion with less odor and stickiness.

Therefore, the present inventors found that the strain of Streptococcus salivarius sbk4 of the present invention isolated and identified in the present invention can be easily utilized in the manufacture of functional cosmetics having excellent antioxidant activity and skin barrier improvement or strengthening activity.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the above description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Korea Research Institute of Bioscience and Biotechnology KCTC13872BP 20190626

<110> Shebah biotech Inc <120> Streptococcus salivarius sbk4 strain isolated from skin and functional cosmetic composition comprising Streptococcus salivarius sbk4 strain <130> NPDC69825.01 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1412 <212> DNA <213> Artificial Sequence <220> <223> Streptococcus salivarius sbk4 16rRNA sequence <400> 1 taatgcagta gaacgctgaa gagaggagct tgctcttctt ggatgagttg cgaacgggtg 60 agtaacgcgt aggtaacctg ccttgtagcg ggggataact attggaaacg atagctaata 120 ccgcataaca atggatgaca catgtcattt atttgaaagg ggcaattgct ccactacaag 180 atggacctgc gttgtattag ctagtaggtg aggtaacggc tcacctaggc gacgatacat 240 agccgacctg agagggtgat cggccacact gggactgaga cacggcccag actcctacgg 300 gaggcagcag tagggaatct tcggcaatgg gggcaaccct gaccgagcaa cgccgcgtga 360 gtgaagaagg ttttcggatc gtaaagctct gttgtaagtc aagaacgagt gtgagagtgg 420 aaagttcaca ctgtgacggt agcttaccag aaagggacgg ctaactacgt gccagcagcc 480 gcggtaatac gtaggtcccg agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc 540 ggtttgataa gtctgaagtt aaaggctgtg gctcaaccat agttcgcttt ggaaactgtc 600 aaacttgagt gcagaagggg agagtggaat tccatgtgta gcggtgaaat gcgtagatat 660 atggaggaac accggtggcg aaagcggctc tctggtctgt aactgacgct gaggctcgaa 720 agcgtgggga gcgaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct 780 aggtgttgga tcctttccgg gattcagtgc cgcagctaac gcattaagca ctccgcctgg 840 ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga 900 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcccgatgct 960 atttctagag atagaaagtt acttcggtac atcggtgaca ggtggtgcat ggttgtcgtc 1020 agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaacccct attgttagtt 1080 gccatcattc agttgggcac tctagcgaga ctgccggtaa taaaccggag gaaggtgggg 1140 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggttggt 1200 acaacgagtt gcgagtcggt gacggcaagc taatctctta aagccaatct cagttcggat 1260 tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga tcagcacgcc 1320 gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac 1380 acccgaagtc ggtgaggtaa cctttggagc ca 1412

Claims (7)

Streptococcus salivarius sbk4 strain isolated from the skin (accession number: KCTC13872BP). The method of claim 1,
The strain is characterized in that it has antioxidant activity, anti-inflammatory activity and skin barrier improving activity. Streptococcus salivarius sbk4 strain (accession number: KCTC13872BP), a culture of the strain, a lysate of the strain or an extract of the strain as an active ingredient, a cosmetic composition having antioxidant activity. The method of claim 3,
The cosmetic composition is characterized in that it has a superoxide radical scavenging ability, ABTS radical scavenging ability or nitrogen monoxide scavenging ability, a cosmetic composition having antioxidant activity. Streptococcus salivarius sbk4 strain (accession number: KCTC13872BP), a culture of the strain, a lysate of the strain or an extract of the strain as an active ingredient, a cosmetic composition for improving or strengthening skin barrier. The method of claim 5,
The cosmetic composition is a cosmetic composition for improving or strengthening the skin barrier, characterized in that to promote or enhance the formation of a cornified envelope. The method of claim 5,
The composition is skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam , Cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, lipstick, makeup base, foundation, press powder, and loose powder for improvement or strengthening of skin barrier, characterized in that formulated as one selected from the group consisting of Cosmetic composition.

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