KR101961653B1 - SNP molecular marker for selecting cultivars of sweet potatoes and uses thereof - Google Patents

Abstract

The present invention relates to a single nucleotide polymorphism (SNP) molecular marker for identifying sweet potato varieties, and a use thereof. To be more specific, the present invention relates to a primer set for amplifying an SNP molecular marker for identifying sweet potato varieties, a kit including the primer set, and a method for identifying sweet potato varieties by using the primer set. According to the present invention, the primer set for amplifying the SNP molecular marker for identifying sweet potato varieties can identify varieties of sweet potatoes and test purity of the same according to the place of origin of the sweet potatoes that are commonly sold, and can be usefully utilized in tests for distinctness, uniformity, and stability of varieties for protecting the varieties and establishing a seed management system. In addition, the SNP molecular marker for identifying sweet potato varieties can be utilized as a marker for securing intellectual property rights of domestic breeding varieties in the import globalization era, and for guaranteeing varieties when registering the variety of a sweet potato in the future. Furthermore, the method for identifying sweet potato varieties comprises: a step a) of separating genome DNA from a sweet potato sample; a step b) of conducting polymerase chain reaction (PCR); and a step c) of detecting a PCR product amplified in the step b).

Description

SNP molecular markers for the identification of sweet potato varieties and their uses {SNP molecular markers for selecting cultivars of sweet potatoes and uses thereof}

The present invention relates to SNP molecular markers for the identification of sweet potato varieties and uses thereof. More particularly, the present invention relates to a primer set for SNP molecular marker amplification for the identification of sweet potato varieties, a kit comprising the primer set, and a method for identifying sweet potato varieties using the primer set.

Most of the crops used for breeding are collecting a large amount of genetic resources every year. The classification of the genetic resources within or within the species is necessary for the management of accurate genetic resources. It is very important for.

In addition, as Korea joined the International Union for the Protection of New Varieties of Plants (UPOV) and the Seed Industry Act came into effect, the necessity of sorting scientific and domestic agricultural products was needed to protect breeder rights and increase farm income. In addition, even in the case of sweet potatoes, a nutritional breeding crop, an identification system with discriminative power of varieties and resources is needed.

Current breeds are distinguished by phenotypic traits based on traditional Mendelian laws and biochemical characteristics such as enzymes and protein mutations. However, such a method has a low frequency of mutation, has limited application to whole crops, and may result in different results depending on the cultivation conditions of the producer. Research. For example, in the Netherlands, DNA profiles for each variety of roses and tomatoes are databaseed, and in Japan, molecular markers for breed identification for rice and other crops have been developed and used to protect varieties.

Molecular markers have the advantage that they can be detected in all tissues by differences in DNA sequence and are not affected by environmental influences and multiple expression of genes. Genomic genetic mapping of major crops is currently underway, and molecular markers are being used in the selection process of breeding. Numerous molecular markers have been developed for the study of plant breeding, and techniques and laboratory equipment for massively verifying and identifying the developed molecular markers are being developed very quickly. A variety of molecular labeling techniques such as Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Simple Sequence Repeat (SSR) and Single Nucleotide Polymorphism (SNP) . The use of a codominant marker in the molecular markers can discriminate homozygous of the gene, and it is possible to carry out a search before the expression of the trait, thereby enabling rapid discrimination in breeding.

Single nucleotide polymorphism (SNP) is a single nucleotide difference in DNA between individuals that exists across a wide variety of species genomes. Frequent SNPs appearing in plant genomes enable gene mapping, marker assisted breeding, and map-based cloning. SNPs are the most common genomic markers, and various SNP detection methods and experimental equipments have been developed in consideration of the ease and cost of the experiment to detect such SNPs.

Korean Patent No. 1403494 discloses 'SSR primer for discriminating sweet potato varieties and a method for discriminating sweet potato varieties using the SSR primer.' The Korean Society of Horticulture (Park, Kwon Woo et al., 5: 112-113, 1996) And the use of the RAPD method for classification. However, SSR (Simple Sequence Repeat) method or Random Amplified Polymorphic DNA (RAPD) method is difficult to discriminate the sweet potato varieties, The reproducibility is low.

On the other hand, in the case of sweet potatoes, the demand for discrimination of sweet potato varieties is increasing rapidly due to patent rights and royalties, but sweet potatoes are difficult to distinguish even in the same varieties. Furthermore, there is no single varietalization of sweet potatoes at present, and it is difficult to unify or standardize the products because they exist in a mixed state.

Therefore, it is necessary to develop a marking system for sweet potato varieties to increase the industrial utilization of sweet potatoes and to develop new markets.

Under these circumstances, the inventors of the present invention derived novel SNP molecular markers through comparative analysis of sweet potato genome sequence and polymorphism in order to develop a sweet potato varietal discriminating marker, and based on this, a primer for SNP molecular marker amplification The present invention has been completed.

Korea Patent No. 1403494

Park, KW et al., The Korean Society of Horticulture, 5: 112-113, 1996

It is an object of the present invention to provide a primer set for SNP molecular marker amplification for the identification of sweet potato varieties, a kit containing the primer set, and a method for distinguishing sweet potato varieties using the primer set.

According to one aspect of the present invention, there is provided a primer set comprising: a primer set represented by SEQ ID NOS: 1, 2, 3 and 4; A primer set represented by SEQ ID NOS: 5, 6, 7, and 8; A primer set represented by SEQ ID NOS: 9, 10, 11, and 12; A primer set represented by SEQ ID NOs: 13, 14, 15, and 16; A primer set represented by SEQ ID NOs: 17, 18, 19, and 20; A primer set represented by SEQ ID NOS: 21, 22, 23 and 24; A primer set represented by SEQ ID NOS: 25, 26, 27, and 28; A primer set represented by SEQ ID NOs: 29, 30, 31, and 32; A primer set represented by SEQ ID NOS: 33, 34, 35, and 36; A primer set represented by SEQ ID NOS: 37, 38, 39, and 40; A primer set represented by SEQ ID NOS: 41, 42, 43, and 44; A primer set represented by SEQ ID NOS: 45, 46, 47, and 48; A primer set represented by SEQ ID NOS: 49, 50, 51, and 52; A primer set represented by SEQ ID NOS: 53, 54, 55, and 56; A primer set represented by SEQ ID NOS: 57, 58, 59, and 60; A primer set represented by SEQ ID NOS: 61, 62, 63 and 64; A primer set represented by SEQ ID NOS: 65, 66, 67, and 68; A primer set represented by SEQ ID NOS: 69, 70, 71 and 72; A primer set represented by SEQ ID NOS: 73, 74, 75, and 76; A primer set represented by SEQ ID NOS: 77, 78, 79 and 80; A primer set represented by SEQ ID NOS: 81, 82, 83 and 84; A primer set represented by SEQ ID NOS: 85, 86, 87 and 88; A primer set represented by SEQ ID NOS: 89, 90, 91, and 92; A primer set represented by SEQ ID NOS: 93, 94, 95, and 96; A primer set represented by SEQ ID NOS: 97, 98, 99 and 100; A primer set represented by SEQ ID NOS: 101, 102, 103, and 104; A primer set represented by SEQ ID NOS: 105, 106, 107, and 108; A primer set represented by SEQ ID NOS: 109, 110, 111, and 112; A primer set represented by SEQ ID NOS: 113, 114, 115, and 116; A primer set represented by SEQ ID NOS: 117, 118, 119, and 120; A primer set represented by SEQ ID NOS: 121, 122, 123, and 124; A primer set represented by SEQ ID NOS: 125, 126, 127, and 128; A primer set represented by SEQ ID NOs: 129, 130, 131, and 132; A primer set represented by SEQ ID NOS: 133, 134, 135, and 136; A primer set represented by SEQ ID NOS: 137, 138, 139, and 140; A primer set represented by SEQ ID NOS: 141, 142, 143 and 144; A primer set represented by SEQ ID NOS: 145, 146, 147, and 148; A primer set represented by SEQ ID NOs: 149, 150, 151, and 152; A primer set represented by SEQ ID NOS: 153, 154, 155, and 156; A primer set represented by SEQ ID NOS: 157, 158, 159, and 160; A primer set represented by SEQ ID NOS: 161, 162, 163, and 164; A primer set represented by SEQ ID NOS: 165, 166, 167, and 168; A primer set represented by SEQ ID NOs: 169, 170, 171, and 172; A primer set represented by SEQ ID NOS: 173, 174, 175, and 176; A primer set represented by SEQ ID NOS: 177, 178, 179, and 180; A primer set represented by SEQ ID NOS: 181, 182, 183, and 184; And a primer set set forth in SEQ ID NOS: 185, 186, 187, and 188.

In another aspect, the present invention provides a kit for discriminating sweet potato varieties comprising the primer set and a reagent for carrying out an amplification reaction.

In another aspect, the present invention provides a method for producing a sweet potato comprising: a) separating genomic DNA from a sweet potato sample; b) performing PCR (polymerase chain reaction) using the separated genomic DNA as a template and using the primer set of claim 1; And c) detecting the amplified PCR product of step b).

Using the primer set for SNP molecular marker amplification for the identification of sweet potato varieties according to the present invention, the identification of varieties and purity according to the origin of sweet potatoes which are commonly used, and the discrimination of varieties for establishing the variety protection and seed management system ), Uniformity, and stability. In addition, SNP molecular markers for the identification of sweet potato varieties according to the present invention can be utilized as a marking factor for the assurance of intellectual property rights for the cultivars cultivated in Korea during the import opening period and the breed guarantee for the registration of the sweet potato variety in the future.

FIG. 1 is a diagram showing genotyping results obtained by analyzing genotypes of SNPs using a Fluidigm platform according to an embodiment of the present invention and using a primer set for SNP molecular marker amplification for identifying sweet potato varieties.
FIG. 2 is a diagram showing a sweet potato genetic circle of various native species distinguished by a primer set for SNP molecular marker amplification for the identification of sweet potato varieties according to an embodiment of the present invention.

More particularly, the present invention relates to a primer set for SNP molecular marker amplification for identifying sweet potato varieties, a kit including the primer set, and a method for discriminating sweet potato varieties using the primer set .

In one aspect, the present invention provides a primer set comprising a set of primers represented by SEQ ID NOS: 1, 2, 3 and 4; A primer set represented by SEQ ID NOS: 5, 6, 7, and 8; A primer set represented by SEQ ID NOS: 9, 10, 11, and 12; A primer set represented by SEQ ID NOs: 13, 14, 15, and 16; A primer set represented by SEQ ID NOs: 17, 18, 19, and 20; A primer set represented by SEQ ID NOS: 21, 22, 23 and 24; A primer set represented by SEQ ID NOS: 25, 26, 27, and 28; A primer set represented by SEQ ID NOs: 29, 30, 31, and 32; A primer set represented by SEQ ID NOS: 33, 34, 35, and 36; A primer set represented by SEQ ID NOS: 37, 38, 39, and 40; A primer set represented by SEQ ID NOS: 41, 42, 43, and 44; A primer set represented by SEQ ID NOS: 45, 46, 47, and 48; A primer set represented by SEQ ID NOS: 49, 50, 51, and 52; A primer set represented by SEQ ID NOS: 53, 54, 55, and 56; A primer set represented by SEQ ID NOS: 57, 58, 59, and 60; A primer set represented by SEQ ID NOS: 61, 62, 63 and 64; A primer set represented by SEQ ID NOS: 65, 66, 67, and 68; A primer set represented by SEQ ID NOS: 69, 70, 71 and 72; A primer set represented by SEQ ID NOS: 73, 74, 75, and 76; A primer set represented by SEQ ID NOS: 77, 78, 79 and 80; A primer set represented by SEQ ID NOS: 81, 82, 83 and 84; A primer set represented by SEQ ID NOS: 85, 86, 87 and 88; A primer set represented by SEQ ID NOS: 89, 90, 91, and 92; A primer set represented by SEQ ID NOS: 93, 94, 95, and 96; A primer set represented by SEQ ID NOS: 97, 98, 99 and 100; A primer set represented by SEQ ID NOS: 101, 102, 103, and 104; A primer set represented by SEQ ID NOS: 105, 106, 107, and 108; A primer set represented by SEQ ID NOS: 109, 110, 111, and 112; A primer set represented by SEQ ID NOS: 113, 114, 115, and 116; A primer set represented by SEQ ID NOS: 117, 118, 119, and 120; A primer set represented by SEQ ID NOS: 121, 122, 123, and 124; A primer set represented by SEQ ID NOS: 125, 126, 127, and 128; A primer set represented by SEQ ID NOs: 129, 130, 131, and 132; A primer set represented by SEQ ID NOS: 133, 134, 135, and 136; A primer set represented by SEQ ID NOS: 137, 138, 139, and 140; A primer set represented by SEQ ID NOS: 141, 142, 143 and 144; A primer set represented by SEQ ID NOS: 145, 146, 147, and 148; A primer set represented by SEQ ID NOs: 149, 150, 151, and 152; A primer set represented by SEQ ID NOS: 153, 154, 155, and 156; A primer set represented by SEQ ID NOS: 157, 158, 159, and 160; A primer set represented by SEQ ID NOS: 161, 162, 163, and 164; A primer set represented by SEQ ID NOS: 165, 166, 167, and 168; A primer set represented by SEQ ID NOs: 169, 170, 171, and 172; A primer set represented by SEQ ID NOS: 173, 174, 175, and 176; A primer set represented by SEQ ID NOS: 177, 178, 179, and 180; A primer set represented by SEQ ID NOS: 181, 182, 183, and 184; And a primer set set forth in SEQ ID NOS: 185, 186, 187, and 188.

In the present invention, the term " discrimination " means discrimination of varieties from sweet potato cultivar samples by the primer set of the present invention to discriminate them and can be used in combination with 'discrimination'.

The term " primer " in the present invention refers to a single-stranded oligonucleotide sequence complementary to a nucleic acid strand to be copied and serves as a starting point for the synthesis of a primer extension product. The length and sequence of the primer should allow for the start of synthesis of the extended product and the specific length and sequence of the primer will depend on the complexity of the desired DNA or RNA target as well as the primer usage conditions such as temperature and ionic strength something to do.

In the present invention, the primer may further include, but is not limited to, a substance that emits intercalating fluorescence, phosphorescence, or radioactive. Preferably, the labeling substance is FAM or HEX. When the target sequence is amplified, FAM or HEX is labeled at the 5'-end of the allele-specific primer, and PCR is carried out, whereby the target sequence can be labeled with a detectable fluorescent labeling substance.

The term " primer set " in the present invention means a plurality of primers. In addition, the primer set may further include a container for receiving the primer. Primer refers to a short nucleotide sequence that can form a base pair with a complementary template with a short free 3 'hydroxyl group and serves as a starting point for template strand copying . The primer can initiate DNA synthesis in the presence of a reagent for polymerization and four nucleoside triphosphates at the appropriate buffer solution and temperature. The primer may be an oligonucleotide and the oligonucleotide may comprise a nucleotide analogue such as phosphorothioate, alkylphosphorothioate or peptide nucleic acid, intercalating agent. The primer can be produced through a chemical synthesis method using a phosphoramidite method, a phosphodiester method, a diethylphosphoramidite method, or the like. In addition, the primer sequence may be modified by means known in the art.

The primer set of the present invention may comprise at least one set of primers selected from the group consisting of a total of 47 sets of primers set forth in SEQ ID NOS: 1 to 188, wherein each set of primers has a set of four primers (Table 3).

The primer set may amplify a polynucleotide comprising the nucleotide sequence of SEQ ID NOS: 189 to 235, and the polynucleotide comprising the nucleotide sequence of SEQ ID NOS: 189 to 235 may comprise a single nucleotide polymorphism (SNP) region for distinguishing between sweet potato varieties (Table 2).

The 'SNP' means that one of the nucleotide sequences consisting of A, T, C, and G on the genome is changed to another nucleotide sequence.

In the present invention, there is provided a method for discriminating sweet potato varieties comprising at least one polynucleotide selected from the polynucleotides consisting of the nucleotide sequences of SEQ ID NOS: 189 to 235 including the SNP (single nucleotide polymorphism) position base or a complementary polynucleotide thereof SNP < / RTI >

SEQ ID NOS: 189 to 235 are polynucleotides comprising the nucleotide sequences of the respective SNP sites. SEQ ID NOS: 189 to 235 are polymorphic sequences including polymorphic sites. A polymorphic sequence refers to a sequence comprising a polymorphic site representing a SNP in a polynucleotide sequence. The polynucleotide sequence may be DNA or RNA.

In the SNP composition according to an embodiment of the present invention, the SNP position base is 21st from SEQ ID NO: 189 to SEQ ID NO: 235, and the polymorphic base information is indicated by [/] in the SNP nucleotide sequence information of Table 2.

The polynucleotide of each SNP constituting SNP for the sweet potato variety according to the present invention is preferably at least 10 continuous bases, more preferably at least 15 continuous bases, even more preferably at least 20 continuous bases And most preferably the respective sequences of SEQ ID NOS: 189 to 235.

The present invention can also provide a microarray for distinguishing sweet potato varieties comprising a polynucleotide of the SNP, a polypeptide encoded thereby, or a cDNA thereof. Preferably, the polynucleotide may be immobilized on a substrate coated with an activator of amino-silane, poly-L-lysine or aldehyde, but is not limited thereto. Preferably, the substrate may be a silicon wafer, glass, quartz, metal or plastic, but is not limited thereto. As a method for immobilizing the polynucleotide on a substrate, a micropipetting method using a piezo electric method, a method using a pin-type spotter can be used.

The microarray according to the present invention can be produced by a conventional method known to those skilled in the art using a polynucleotide according to the present invention or a complementary polynucleotide thereof, a polypeptide encoded thereby, or cDNA thereof.

In another aspect, the present invention provides a kit for discriminating sweet potato varieties comprising the primer set and a reagent for carrying out an amplification reaction.

In the kit of the present invention, the reagent for carrying out the amplification reaction may include a DNA polymerase, dNTPs and a buffer. The dNTP mixture may include dATP, dCTP, dGTP, dTTP, and the heat-resistant DNA polymerase may be a commercially available heat-resistant DNA polymerase such as Taq DNA polymerase or Tth DNA polymerase. In addition, the kit of the present invention may further include a user guide describing optimal reaction performing conditions. The manual is a printed document that explains how to use the kit, for example, how to prepare PCR buffer, the reaction conditions presented, and so on. The manual includes instructions on the surface of the package including a brochure or leaflet in the form of a brochure, a label attached to the kit, and a kit. In addition, the brochure includes information that is disclosed or provided through an electronic medium such as the Internet.

In another aspect, the present invention provides a method for producing a sweet potato comprising: a) separating genomic DNA from a sweet potato sample; b) performing PCR (polymerase chain reaction) using the separated genomic DNA as a template and using the primer set of claim 1; And c) detecting the amplified PCR product of step b).

In the sweet potato variety identification method of the present invention, the sweet potato sample may be, but not limited to, the sweet potato resource 67 (variety 56 resource and native species 11 resource) of various native products shown in Table 1 below.

In the method of the present invention, the method of isolating the genomic DNA from the sweet potato sample can be performed by a conventional method known in the art, and the method can be selected from phenol / chloroform extraction method, SDS extraction method (Tai et al., Plant Mol. Biol Reporter, 8: 297-303, 1990), Cetyl Trimethyl Ammonium Bromide (Murray et al., Nuc. Res., 4321-4325, 1980), or commercially available DNA extraction kits .

The target sequence may be amplified by performing amplification reaction using the genomic DNA of the separated sweet potato sample as a template and using at least one primer set according to an embodiment of the present invention. Methods for amplifying a target nucleic acid include polymerase chain reaction (PCR), ligase chain reaction, nucleic acid sequence-based amplification, transcription-based amplification system, Strand displacement amplification or amplification with Q [beta] replicase, or any other suitable method for amplifying nucleic acid molecules known in the art. Among them, PCR is a method of amplifying a target nucleic acid from a pair of primers that specifically bind to a target nucleic acid using a polymerase. Such PCR methods are well known in the art, and commercially available kits may be used. The PCR can be carried out using a PCR reaction mixture containing various components known in the art necessary for the PCR reaction. The PCR reaction mixture includes a suitable amount of DNA polymerase, dNTP, PCR buffer solution and water in addition to the genomic DNA extracted from sweet potato samples to be analyzed and the primer set provided in the present invention. The PCR buffer comprises Tris -HCl (Tris-HCl), MgCl 2, KCl and the like.

In the method of the present invention, the amplified target sequence may be labeled with a detectable labeling substance. The labeling substance may be a fluorescent, phosphorescent or radioactive substance, but is not limited thereto. The labeling substance may be FAM or HEX. When the target sequence is amplified, PCR is carried out by labeling FAM or HEX at the 5'-end of the primer, and the target sequence may be labeled with a detectable fluorescent labeling substance. When the radioactive isotope such as ³² P or ³⁵ S is added to the PCR reaction solution, the amplification product may be synthesized and the radioactive substance may be incorporated into the amplification product and the amplification product may be labeled as radioactive. One or more sets of primers used to amplify the target sequence are as described above.

In the method of the present invention, the method for distinguishing sweet potato varieties comprises detecting the amplification product, wherein the amplification product is detected by sequencing, DNA chip, gel electrophoresis, radioactivity measurement, fluorescence measurement or phosphorescence measurement But is not limited thereto. As one method of detecting the amplification product, gel electrophoresis can be performed. Gel electrophoresis can be performed using agarose gel electrophoresis or acrylamide gel electrophoresis depending on the size of the amplification product. In addition, in the fluorescence measurement method, FAM or HEX is labeled at the 5'-end of the primer and when the PCR is performed, the target sequence is labeled with a fluorescent label capable of detecting the fluorescence, and the fluorescence thus labeled can be measured using a fluorescence analyzer. In addition, in the case of performing the PCR, the radioactive isotope such as ³² P or ³⁵ S is added to the PCR reaction solution to mark the amplification product, and then a radioactive measurement device such as a Geiger counter or liquid scintillation counter The radioactivity can be measured using a liquid scintillation counter.

Hereinafter, the constitution and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1: Material preparation and DNA extraction

Example 1-1: Test material

In order to develop the SNP molecular markers for sweet potato varieties, 67 varieties of native potatoes (56 varieties and 11 indigenous varieties) under the National Register of the National Institute of Food Science and Technology were used.

No. Sweet potato genotype No. 1. Sweet potato variety name origin One IT232005 Chunmi Japan 2 IT232010 Won Japan 3 IT232012 Jang Bin Japan 4 IT232016 The Japan 5 IT232017 Yeah Japan 6 IT232027 Sincerity Korea 7 IT232036 Hwangmi Korea 8 IT232071 Agriculture forest 13 Japan 9 IT232077 Agriculture forest 22 Japan 10 IT232080 Agriculture forest 26 Japan 11 IT232091 Daenong No.10 Taiwan 12 IT232095 Dainong 32 Taiwan 13 IT232098 WhiteStar United States of America 14 IT232099 YellowJersey United States of America 15 IT232100 Earlypost United States of America 16 IT232105 Nugget United States of America 17 IT232128 Jasper United States of America 18 IT232134 Hermit Korea 19 IT232165 Delicacy Korea 20 IT232167 Stern Korea 21 IT232169 Raw Korea 22 IT232179 White Korea 23 IT232180 Goto Rum Korea 24 IT232190 zest Korea 25 IT232191 Shrink Korea 26 IT232215 Fanshul Philippines 27 IT232222 Wall Korea 28 IT232223 Jeongeup Korea 29 IT232225 Jeju traditional Korea 30 IT232226 Dangjin Korea 31 IT232228 Foliage -2 Korea 32 IT232234 Benny Hayato Japan 33 IT232236 Mountaineer Japan 34 IT232239 No. 98 Kanto Japan 35 IT232274 Benny Chima Japan 36 IT232275 Beniakis Japan 37 IT232278 Benisa Satsuma Japan 38 IT232321 Sweetness Korea 39 IT232324 Jami Korea 40 IT232328 Sincheon mi Korea 41 IT232331 Nanjing China 42 IT232332 Nanjing 40 China 43 IT232338 Lizixiang China 44 IT232339 Yu7 China 45 IT232345 Simon China 46 IT232348 AYAMURA SAKU China 47 IT258174 CIP420051 Peru 48 IT258176 CIP420089 Peru 49 IT258177 CIP440027 Peru 50 IT258178 CIP440031 Peru 51 IT258179 CIP440038 Peru 52 IT258182 CIP440078 Peru 53 IT258184 CIP440104 Peru 54 IT258186 CIP440115 Peru 55 IT258187 CIP440116 Peru 56 IT258190 CIP440120 Peru 57 IT258202 CIP440188 Peru 58 IT258203 CIP440194 Peru 59 IT258207 CIP440223 Peru 60 IT258213 Shin Kun Mi Korea 61 IT258215 Go Kun Mi Korea 62 IT258229 Jindo 1 Korea 63 IT258231 Jindo 3 Korea 64 IT258233 Baekryong Traditional Korea 65 IT258238 HCM Korea 66 IT258240 Naju Nara 1 Korea 67 IT258243 Yeoju Collection Korea

Example 1-2: DNA extraction

Genomic DNA of the plants was extracted using the Gentra Puregene Cell kit for plant (QIAGEN) for the potato varieties of 67 resources of Example 1-1. The DNA of each sweet potato extracted was checked for the quality and quantity of sweet potato DNA using ND-1000 spectrometer (NanoDrop Technologies, Wilmington, DE, USA), and the DNA concentration was determined to be 50 ng / Respectively.

Example 2: Development of novel SNP molecular markers for sweet potato variety identification and primer design

In order to develop a new SNP molecular marker for the identification of sweet potato varieties, a variety of 96 native potatoes (56 varieties and 11 native varieties) prepared in Example 1-1 were subjected to GIP (Genotyping By Sequencing) The candidate SNP (Single Nucleotide Polymorphism) site and surrounding nucleotide sequence information were obtained. An amplification primer set for allelic loci derived from the obtained candidate SNP region was prepared and used for discrimination of sweet potato varieties using Fluidigm instrument. Genetic differences between the target candidates of the 96 candidate SNP regions were clearly observed. Based on the results, the amplification primers of allelic loci for 47 SNP regions, which can be used for the identification of sweet potato varieties, (Table 2 and Table 3).

47 SNP molecular marker information for discriminating sweet potato varieties of the present invention No. Marker name
(Marker name) SNP molecular marker base sequence information (5 '- >3')
Left flanking sequence [A / B] Right flanking sequence SEQ ID NO: One SP_SNP_1 CAGTAATCGGGATCAAGAAG [G / T] CCCCGGTTAGGGACATAGTT 189 2 SP_SNP_2 TTCACCAGAGTTACTTCCTT [C / T] GCTTTCTGACAACTCATTAC 190 3 SP_SNP_3 GGTGTGCGAGGGCGACCTGA [C / T] GAATGTCGGGGCTGCTGCGG 191 4 SP_SNP_4 GATGAATATGTCCATGGAAA [C / A] CAGGATCAATATCCATGTTT 192 5 SP_SNP_5 ATGGCGTAAACTCAGCATGG [T / C] TACAAAGAGCTTTTTCTTGA 193 6 SP_SNP_6 TCTATTGCAGCAGCCTTTTT [C / A] ACATTGGAGAACTCGGAACT 194 7 SP_SNP_7 AAGAAAGCAGTGGAAGCAAT [C / A] TGCCCCGGTGTTGTCTCGTG 195 8 SP_SNP_8 CATTTGAAACTGATCAATAA [G / A] CAACTTACATAAGAGACACC 196 9 SP_SNP_9 TCATCAGGAGAAGGATACAC [A / T] ATGTTATTTACCAAAGTGAA 197 10 SP_SNP_10 ACCGCAATTCCTGAGCTTCT [A / G] TGTGAGTTTATTTATTTGCG 198 11 SP_SNP_11 TCAAATTATGTTGGATGAGA [G / A] ATGATGCAGCGTCCACAATG 199 12 SP_SNP_12 GTGGCTGTGGATGCGGTGGC [C / G] GTGGAGGCGGAGGCAGTGGC 200 13 SP_SNP_13 TCTGTCTTAACTGCAGATTC [A / C] TCCATGGGCACTGCTGATTG 201 14 SP_SNP_14 TAATTTTTCATAAGTAGTTG [G / T] AAGGTTGGATATAACAATTT 202 15 SP_SNP_15 GTAAGGTCACACTGCGCTGA [A / C] AAATCTGAGAATTCCCCACT 203 16 SP_SNP_16 TTACTGTCTCTTTTGAATAA [A / T] GTTTGGTTTCATAAGATAAA 204 17 SP_SNP_17 GCAGCTATGGTTTTAGCTCT [G / C] TGCTCATGAAAGGCCATCAA 205 18 SP_SNP_18 TGCCTCAATCGTATATAGGA [C / T] AACCAGAATGAGATCATACA 206 19 SP_SNP_19 ACTAGCAGGAATCCTAATTC [C / A] TAGCCTTTAAACGACTTGAC 207 20 SP_SNP_20 TCTGCAGAACCAATTGCAAT [G / A] CTCATTCTCCGCTTCCAATC 208 21 SP_SNP_21 GGTATTGCAGCGTTTACACC [A / T] TCGCCAAGGGGAACAAGGTG 209 22 SP_SNP_22 ATAACACACAATGGGAATAC [A / G] AAAGGAATTACTCCGTAATA 210 23 SP_SNP_23 CAAATATGTGGGTCATCCTT [T / G] CATTTGTACGACAAAGATCA 211 24 SP_SNP_24 GGTGAGGACGTTTGATCCCA [C / G] AGAGATTTCTGGAGAATTTA 212 25 SP_SNP_25 AATTAATTTTGAAGAAATAT [T / G] TATATAAGCCTTGGCACGTT 213 26 SP_SNP_26 TTCGTCATACCATATAGGAT [A / G] GAGAATTGAAAGTGACAATC 214 27 SP_SNP_27 AGCTTTGATTACCCGACGGA [C / T] ACATTGCTGCCGGGTATGAA 215 28 SP_SNP_28 CCAAGAAGCTGAAACGAGTC [G / A] CTGCTCATCTCGGCCACGAA 216 29 SP_SNP_29 CAAGGATATTCAGGTAGACA [G / A] GGATCAGTCAGTTGCTCAGT 217 30 SP_SNP_30 TTTGGCTCCCCCAGTTTAGC [G / T] GTCCTTGTGAAGAACACACC 218 31 SP_SNP_31 CCTTTGGGCAGTGCATCGGA [A / G] CAAGGTGGATGAGTCCAACT 219 32 SP_SNP_32 CTCCAACCTTGTAGGTCTT [C / G] CTGTTTCATGCTTTATTTAT 220 33 SP_SNP_33 AGCATTGATCATTAACTCGT [A / G] GGAAATGCGGCCAACTCTTT 221 34 SP_SNP_34 ATCTTTAGCTTGTTAGATAG [C / T] GCTTTCTTGTCATGGTTGGA 222 35 SP_SNP_35 TCAGTTTCAGAGGTTGAAGT [A / G] ATTCACGGTCACATTGTAAA 223 36 SP_SNP_36 TCATATTAATGGAAGCAGTA [G / A] GTGCATTCTTTCCTTTGGGA 224 37 SP_SNP_37 GCTGCTGAGAACTATCATCG [C / G] TCTTGGTGGAAGAGGCATGG 225 38 SP_SNP_38 CCCAAACAGGATCGCTATGC [C / T] CTCCGCACTTCTCCCCAATG 226 39 SP_SNP_39 TACCGCTGCACGGTGGCGCC [A / G] TCGTGCCCGGTGAGAAAACA 227 40 SP_SNP_40 CAGTTGTGACATTATATTTG [C / T] AATGAAAAATCATTAACTTA 228 41 SP_SNP_41 CCAGCCTAAAGAAAACGCAT [C / A] GAGCACAAGGCTTCACCGAG 229 42 SP_SNP_42 ATGACTGTTTCTTCTCTGTT [T / G] TGCTGTGATTATATTCATTC 230 43 SP_SNP_43 ACATGGTAAGAGATGAGCCC [G / A] ACACTGCAACCCTCAACGAC 231 44 SP_SNP_44 TTAATGTACGCCCCATGTGT [A / T] TGTTCAGGTCCTATTGCAGC 232 45 SP_SNP_45 GAAGTGAAAATCAATACGAA [A / G] TAGATAATGAGGTGAACTGA 233 46 SP_SNP_46 GCGATCAGGCGACCGCCGCG [C / T] GAAATCGAGCGCGTTCTTCA 234 47 SP_SNP_47 TTCTACAATGGGTACTGCAA [C / T] GGTTATGCATATGAAGAAGA 235

* [A / B]: means that nucleotide A has a single nucleotide polymorphism (SNP) that is point mutated to base B.

Sequencing of primers for amplifying 47 SNP molecular markers for the identification of sweet potato varieties No. Marker name
(Marker name) SNP * Forward primer order
number Reverse primer order
number A1 specific primer order
number A2 specific primer order
number A1 A2 One SP_SNP_1 G T ACAGACCTCAGGATTGGGAAAA One CATAGAGCTCGGGAGGGCA 2 CTATGTCCCTAACCGGGGC 3 ACTATGTCCCTAACCGGGGA 4 2 SP_SNP_2 C T ATGCGCGCAAGCAGTT 5 ACCACGGAAGCAGCTAGGAT 6 GCCTTCACCAGAGTTACTTCCTTC 7 GCCTTCACCAGAGTTACTTCCTTT 8 3 SP_SNP_3 C T CGTCTCTTCTTTCCCCGCA 9 CTGGAACTCCGGTAGGGTACT 10 AGCAGCCCCGACATTCG 11 AGCAGCCCCGACATTCA 12 4 SP_SNP_4 C A GCGAAGTTCCAGCCCC 13 GCAGCCTGTGTTGCATGTGT 14 GTGTCATGATGAATATGTCCATGGAAAC 15 GTGTCATGATGAATATGTCCATGGAAAA 16 5 SP_SNP_5 T C TCGTTTCTTCTCCTGAACGGA 17 CACGAGTGCCACTGAAATCCAA 18 TGGCGTAAACTCAGCATGGT 19 GGCGTAAACTCAGCATGGC 20 6 SP_SNP_6 C A CAAACTTGTTTTGGAAAGAGCGTC 21 TTGGTGGCCTGAGTTCCGA 22 CGTAATCTATTGCAGCAGCCTTTTTC 23 CGTAATCTATTGCAGCAGCCTTTTTA 24 7 SP_SNP_7 C A CCTGCATGCATTCTATGTCATTGAT 25 CAGCACACGAGACAACACCG 26 GCAAAGAAAGCAGTGGAAGCAATC 27 GCAAAGAAAGCAGTGGAAGCAATA 28 8 SP_SNP_8 G A CCGCAGCTATACATGGTTGAA 29 CAAACAGGATGGAATCATTTGAAACTGATCAA 30 CTTCAGGTGTCTCTTATGTAAGTTGC 31 CCTTCAGGTGTCTCTTATGTAAGTTGT 32 9 SP_SNP_9 A T AGCATGGGATGAGATAGATCCAA 33 CTTGGAGGCTGCAATCTGCT 34 CCAAAATCATCAGGAGAAGGATACACA 35 CCAAAATCATCAGGAGAAGGATACACT 36 10 SP_SNP_10 A G CACAACGGATAACAAAAGAAAGAGGA 37 GATGATCTGTACACGCAAATAAATAAACTCACA 38 AACCGCAATTCCTGAGCTTCTA 39 CCGCAATTCCTGAGCTTCTG 40 11 SP_SNP_11 G A TGTTAGAACATGTTTGACCAGCA 41 CTCGTTGCATTGTGGACGCT 42 CCGTCTCAAATTATGTTGGATGAGAG 43 ACCGTCTCAAATTATGTTGGATGAGAA 44 12 SP_SNP_12 C G CGCAGCCACTGCCTC 45 GCGGCAGGGTTGCGAA 46 GCCTCCGCCTCCACG 47 GCCTCCGCCTCCACC 48 13 SP_SNP_13 A C GCTGAAGAAAGCAAGCAGCA 49 AGTGAATCGGCTCCGAAGGAA 50 ATCAGCAGTGCCCATGGAT 51 TCAGCAGTGCCCATGGAG 52 14 SP_SNP_14 G T CCTCTGTGCAATAAAATCAGAGGA 53 GCAGCACTATTTTGATTACCTCTGATAATTTTTCAT 54 ACAGTTGAAAGAAATTGTTATATCCAACCTTC 55 GACAGTTGAAAGAAATTGTTATATCCAACCTTA 56 15 SP_SNP_15 A C TGACTTCCCTTCCACGGTAA 57 CCCGAACAGAGCTACCTAGCA 58 AGGTCACACTGCGCTGAA 59 AGGTCACACTGCGCTGAC 60 16 SP_SNP_16 A T CTGGCTCAGTTTAATTCATTGCAC 61 CCCCAGGGCATAATCCTGAGA 62 TGATTCTTTAAAGCTTTACTGTCTCTTTTGAATAAA 63 TGATTCTTTAAAGCTTTACTGTCTCTTTTGAATAAT 64 17 SP_SNP_17 G C ACAAGCATTGAAGAGTCTTCTTCAG 65 GGTCAAATAATAGCAGCTATGGTTTTAGCTC 66 TTTGATGGCCTTTCATGAGCAC 67 GTTTGATGGCCTTTCATGAGCAG 68 18 SP_SNP_18 C T CAGATAATAGAGCATGGTGCACA 69 AGCAGCGGGTTACGGGA 70 ACCATATGCCTCAATCGTATATAGGAC 71 CACCATATGCCTCAATCGTATATAGGAT 72 19 SP_SNP_19 C A CATCAACAAGATTCAGGAATCCCA 73 CCCACACTGCAGCTAGCTAGT 74 CCCACTAGCAGGAATCCTAATTCC 75 CCCACTAGCAGGAATCCTAATTCA 76 20 SP_SNP_20 G A AACTTACGCGATCCCCTCT 77 CACGGGCAGCATTCAACTGA 78 CCTCTGCAGAACCAATTGCAATG 79 CCTCTGCAGAACCAATTGCAATA 80 21 SP_SNP_21 A T GTTGCAAGCACAGTGATGAAAG 81 CAGAAGCCACCTTGTTCCCC 82 GGTATTGCAGCGTTTACACCA 83 GGTATTGCAGCGTTTACACCT 84 22 SP_SNP_22 A G AACCATCACTACAATAAGCATGGG 85 CGACGAGGCAGCAGACTGTA 86 TGGGTATAACACACAATGGGAATACA 87 TGGGTATAACACACAATGGGAATACG 88 23 SP_SNP_23 T G GAGCATGGCTGCGTATCTG 89 GTTTGATCACTGCACGTCTCCATT 90 GCAAATATTACAAATATGTGGGTCATCCTTT 91 GCAAATATTACAAATATGTGGGTCATCCTTG 92 24 SP_SNP_24 C G GCGATTTGCCTGCTGCG 93 AGCCATGGAGCTGAGACTGAA 94 GGTGAGGACGTTTGATCCCAC 95 GGTGAGGACGTTTGATCCCAG 96 25 SP_SNP_25 T G AGCACATCCGAGATCAGAAATTTT 97 TGCAGCGTGTAATACTTGCTTTTGT 98 TGGAACGTGCCAAGGCTTATATAA 99 TGGAACGTGCCAAGGCTTATATAC 100 26 SP_SNP_26 A G ACACATGGCAGCATGTTTACG 101 TGGCACATTCCCAGTACTGGT 102 GATGTGGATTGTCACTTTCAATTCTCT 103 GATGTGGATTGTCACTTTCAATTCTCC 104 27 SP_SNP_27 C T GAATCCGGAGAATTATCTGTGGC 105 TCCCACCCGAGTTTCATACCC 106 AGCTTTGATTACCCGACGGAC 107 AGCTTTGATTACCCGACGGAT 108 28 SP_SNP_28 G A TCCCTCGTTCGGCCATG 109 CAGCCGTGTCGAACATAGGG 110 CCAAGAAGCTGAAACGAGTCG 111 GCCAAGAAGCTGAAACGAGTCA 112 29 SP_SNP_29 G A TTGCAATAAATCCAGAGAATGGGAAT 113 GCCACCGGCTTTGTTCACA 114 TTCAACCAAGGATATTCAGGTAGACAG 115 TTCAACCAAGGATATTCAGGTAGACAA 116 30 SP_SNP_30 G T CAGCAAATGGCAAGTGAGAGA 117 TGTCTTTGGCTCCCCCAGT 118 AGGTGTGTTCTTCACAAGGACC 119 AGGTGTGTTCTTCACAAGGACA 120 31 SP_SNP_31 A G CGATGAACGTATGCCAGAATGC 121 GACGTACAGCCAAGCAGCC 122 CAGTTGGACTCATCCACCTTGT 123 AGTTGGACTCATCCACCTTGC 124 32 SP_SNP_32 C G CCAACTCAGGTAAAGCTACATGAA 125 AGGGAATGCAATCATAAATAAAGCATGAAACA 126 AACTATCTCCAACCATTGTAGGTCTTC 127 ACTATCTCCAACCATTGTAGGTCTTG 128 33 SP_SNP_33 A G TCTGAAGGAGCAGCAGGA 129 AGCACTGTGTAGTGGACAGCA 130 AGAGTTGGCCGCATTTCCT 131 GAGTTGGCCGCATTTCCC 132 34 SP_SNP_34 C T CCTCTTTCTGCTGCTGCT 133 TGTCCCCGTTCAATACCAGCT 134 CTCCAACCATGACAAGAAAGCG 135 CTCCAACCATGACAAGAAAGCA 136 35 SP_SNP_35 A G GACAAATGAGTCCTGATATGAACCA 137 CCGGTGTTGCGAGAATGTGT 138 CAGTCTTTACAATGTGACCGTGAATT 139 CAGTCTTTACAATGTGACCGTGAATC 140 36 SP_SNP_36 G A GCCAAGAAGGGTCCTGC 141 GGAATCACTATAACTATCTCTCATATTAATGGAAGCA 142 CTCCCAAAGGAAAGAATGCACC 143 CTCCCAAAGGAAAGAATGCACT 144 37 SP_SNP_37 C G GCAATTTCACCATCAAGAACAACTC 145 GCTGTCAAAGGGTGCTGCT 146 CCATGCCTCTTCCACCAAGAG 147 CCATGCCTCTTCCACCAAGAC 148 38 SP_SNP_38 C T GAAATGGATCCTTTGCAGAAACC 149 AATCTGAGGGCCGAGCCA 150 CCAAACAGGATCGCTATGCC 151 CCCAAACAGGATCGCTATGCT 152 39 SP_SNP_39 A G GCACATCGTTGCACCTGT 153 GGAATCCATGCCCGCGT 154 TTTTCTCACCGGGCACGAT 155 TTCTCACCGGGCACGAC 156 40 SP_SNP_40 C T GTCTGTTATTTGCTTTAAAGTCCGC 157 TTCACTATTGAGCAGCAAATAAGTTAATGATTTTTCA 158 TCCGCAGTTGTGACATTATATTTGC 159 GTCCGCAGTTGTGACATTATATTTGT 160 41 SP_SNP_41 C A GTTCTCGATCCCCTCGTTG 161 CCACTCCCATCCTCTGGAGC 162 GCCAGCCTAAAGAAAACGCATC 163 GCCAGCCTAAAGAAAACGCATA 164 42 SP_SNP_42 T G TGCTGCCTATATGATCGACTGAG 165 GCACTCCAATCTGCAAAAGCATATCA 166 GATGATGAAATGACTGTTTCTTCTCTGTTT 167 ATGATGAAATGACTGTTTCTTCTCTGTTG 168 43 SP_SNP_43 G A CGCAGATATTGGGAAGCTGATAA 169 ACGTCCATTGATGAGGCTGGA 170 ACATGGTAAGAGATGAGCCCG 171 ATAAACATGGTAAGAGATGAGCCCA 172 44 SP_SNP_44 A T GGCAGAGAGCATGTGCAATAATAA 173 AGGCTGCAATAGGACCTGAACA 174 GGTTTAATGTACGCCCCATGTGTA 175 GTTTAATGTACGCCCCATGTGTT 176 45 SP_SNP_45 A G TCGATGTTCCTGATAACCTCAGTAC 177 TGCAGCAACATAGACATACTGAAGTGA 178 GACTGGTCAGTTCACCTCATTATCTAT 179 GACTGGTCAGTTCACCTCATTATCTAC 180 46 SP_SNP_46 C T GGCAGAATGCACGTCGATTT 181 GGCGATCAGGCGACCG 182 GAAGAACGCGCTCGATTTCG 183 TGAAGAACGCGCTCGATTTCA 184 47 SP_SNP_47 C T CTGTGAAGGAACGTCGTTTAGTAAT 185 ACGCCCACCCTTTCTCCT 186 GGTTTCTACAATGGGTACTGCAAC 187 TGGTTTCTACAATGGGTACTGCAAT 188

* A1: the first allele in the SNP region.

A2: A second allele at the SNP site.

Example 3: Genetic diversity evaluation and genotyping analysis in sweet potato varieties

In order to evaluate the usefulness of a primer set for SNP molecular marker amplification for the identification of sweet potato varieties newly developed in Example 2 and to study the genetic diversity among various native sweet potato 67 resources (56 varieties and 11 indigenous varieties), PowerMarker Major allele frequency (MAF), genetic diversity (GD), and polymorphic information content (PIC) were analyzed using the data set (ver 3.25). In addition, phylogenetic analysis was performed by using the shared allele genetic distance included in PowerMarker to analyze the genetic distances of 67 resources of sweet potato, and then creating a phylogenetic tree using the UPGMA method. Genotype analysis of each SNP was performed using a Fluidigm instrument, and genotyping was performed using a primer set for amplifying a SNP molecular marker for the identification of the newly developed sweet potato varieties in the potato 67 resource 1). After that, the tree species were finally identified and the varieties of 67 different potatoes of various native species were identified and analyzed.

Genetic diversity analysis of 67 varieties of sweet potato varieties using primer set for SNP molecular markers for identification of newly developed sweet potato varieties revealed that the mean MAF (major allele frequency) was 0.674 and 0.993 at the SP-SNP_11 site , Respectively. Genetic diversity (GD) and polymorphism informatino content (PIC), which indicate the genetic diversity of the markers used in the analysis, ranged from 0.015 to 0.500 and 0.015 to 0.375, respectively. SP_SNP_9, SP_SNP_24, SP_SNP_29, SP_SNP_34, SP_SNP_35, SP_SNP_37 , SP_SNP_38, SP_SNP_39, and SP_SNP_45 showed the highest values for both GD and PIC (Table 4).

Also, genotype analysis of each SNP using the Fluidigm platform was performed using the primer set for SNP molecular marker amplification for the sweet potato variety identification, and the phylogenetic tree was created and analyzed based on the genotyping result. As a result, A total of 67 resources in the wild were clearly distinguished according to the genotype of the sweet potato target resources (Fig. 2).

Accordingly, it was found that the newly developed SNP molecular markers for distinguishing sweet potato varieties and their primer sets for amplification can be used for distinguishing and distinguishing sweet potato varieties of various native ones.

Characteristics of 47 SNP molecular markers in various sweet potato varieties and traditional resources No. Marker name
(Marker name) GN ^a) MAF ^b) GD ^c) OH ^d) PIC ^e) One SP_SNP_1 2 0.697 0.422 0.606 0.333 2 SP_SNP_2 2 0.649 0.455 0.701 0.352 3 SP_SNP_3 3 0.888 0.199 0.194 0.179 4 SP_SNP_4 2 0.910 0.163 0.179 0.150 5 SP_SNP_5 2 0.522 0.499 0.955 0.374 6 SP_SNP_6 3 0.560 0.493 0.851 0.371 7 SP_SNP_7 2 0.582 0.487 0.836 0.368 8 SP_SNP_8 2 0.672 0.441 0.657 0.344 9 SP_SNP_9 2 0.515 0.500 0.970 0.375 10 SP_SNP_10 2 0.843 0.264 0.313 0.229 11 SP_SNP_11 2 0.993 0.015 0.015 0.015 12 SP_SNP_12 3 0.634 0.464 0.701 0.356 13 SP_SNP_13 3 0.667 0.444 0.606 0.346 14 SP_SNP_14 3 0.582 0.487 0.806 0.368 15 SP_SNP_15 2 0.866 0.233 0.269 0.206 16 SP_SNP_16 2 0.806 0.313 0.388 0.264 17 SP_SNP_17 2 0.530 0.498 0.940 0.374 18 SP_SNP_18 2 0.560 0.493 0.881 0.371 19 SP_SNP_19 2 0.888 0.199 0.224 0.179 20 SP_SNP_20 2 0.716 0.406 0.567 0.324 21 SP_SNP_21 2 0.970 0.058 0.000 0.056 22 SP_SNP_22 2 0.985 0.029 0.030 0.029 23 SP_SNP_23 3 0.545 0.496 0.881 0.373 24 SP_SNP_24 3 0.500 0.500 0.970 0.375 25 SP_SNP_25 2 0.537 0.497 0.925 0.374 26 SP_SNP_26 2 0.652 0.454 0.697 0.351 27 SP_SNP_27 3 0.530 0.498 0.851 0.374 28 SP_SNP_28 2 0.806 0.313 0.388 0.264 29 SP_SNP_29 2 0.507 0.500 0.985 0.375 30 SP_SNP_30 3 0.537 0.497 0.896 0.374 31 SP_SNP_31 2 0.694 0.425 0.612 0.335 32 SP_SNP_32 2 0.903 0.175 0.194 0.160 33 SP_SNP_33 2 0.634 0.464 0.731 0.356 34 SP_SNP_34 2 0.507 0.500 0.985 0.375 35 SP_SNP_35 3 0.507 0.500 0.955 0.375 36 SP_SNP_36 3 0.978 0.044 0.015 0.043 37 SP_SNP_37 2 0.515 0.500 0.970 0.375 38 SP_SNP_38 2 0.507 0.500 0.985 0.375 39 SP_SNP_39 2 0.515 0.500 0.970 0.375 40 SP_SNP_40 2 0.784 0.339 0.433 0.282 41 SP_SNP_41 3 0.537 0.497 0.896 0.374 42 SP_SNP_42 2 0.712 0.410 0.576 0.326 43 SP_SNP_43 2 0.948 0.099 0.104 0.094 44 SP_SNP_44 2 0.567 0.491 0.866 0.370 45 SP_SNP_45 2 0.507 0.500 0.985 0.375 46 SP_SNP_46 2 0.552 0.495 0.896 0.372 47 SP_SNP_47 2 0.888 0.199 0.224 0.179 Mean 2.1 0.674 0.379 0.642 0.293

^a) GN (Genotype Number): number of genotypes; ^b) MAF (major allele frequency): Major allele frequency; ^c) Genetic diversity (GD): genetic diversity; ^d) OH (Observed heterozygosity): observed heterozygosity expectation; ^e) Polymorphic information content (PIC): Polymorphic information.

<110> Republic of Korea <120> SNP molecular marker for selecting cultivars of sweet potatoes          and uses <130> P17R12C1235 <160> 235 <170> KoPatentin 3.0 <210> 1 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_1_F <400> 1 acagacctca ggattgggaa aa 22 <210> 2 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_1_R <400> 2 catagagctc gggagggca 19 <210> 3 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_1_A1 <400> 3 ctatgtccct aaccggggc 19 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_1_A2 <400> 4 actatgtccc taaccgggga 20 <210> 5 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_2_F <400> 5 atgcgcgcaa gcagtt 16 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_2_R <400> 6 accacggaag cagctaggat 20 <210> 7 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_2_A1 <400> 7 gccttcacca gagttacttc cttc 24 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_2_A2 <400> 8 gccttcacca gagttacttc cttt 24 <210> 9 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_3_F <400> 9 cgtctcttct ttccccgca 19 <210> 10 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_3_R <400> 10 ctggaactcc ggtagggtac t 21 <210> 11 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_3_A1 <400> 11 agcagccccg acattcg 17 <210> 12 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_3_A2 <400> 12 agcagccccg acattca 17 <210> 13 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_4_F <400> 13 gcgaagttcc agcccc 16 <210> 14 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_4_R <400> 14 gcagcctgtg ttgcatgtgt 20 <210> 15 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_4_A1 <400> 15 gtgtcatgat gaatatgtcc atggaaac 28 <210> 16 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_4_A2 <400> 16 gtgtcatgat gaatatgtcc atggaaaa 28 <210> 17 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_5_F <400> 17 tcgtttcttc tcctgaacgg a 21 <210> 18 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_5_R <400> 18 cacgagtgcc actgaaatcc aa 22 <210> 19 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_5_A1 <400> 19 tggcgtaaac tcagcatggt 20 <210> 20 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_5_A2 <400> 20 ggcgtaaact cagcatggc 19 <210> 21 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_6_F <400> 21 caaacttgtt ttggaaagag cgtc 24 <210> 22 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_6_R <400> 22 ttggtggcct gagttccga 19 <210> 23 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_6_A1 <400> 23 cgtaatctat tgcagcagcc tttttc 26 <210> 24 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_6_A2 <400> 24 cgtaatctat tgcagcagcc ttttta 26 <210> 25 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_7_F <400> 25 cctgcatgca ttctatgtca ttgat 25 <210> 26 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_7_R <400> 26 cagcacacga gacaacaccg 20 <210> 27 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_7_A1 <400> 27 gcaaagaaag cagtggaagc aatc 24 <210> 28 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_7_A2 <400> 28 gcaaagaaag cagtggaagc aata 24 <210> 29 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_8_F <400> 29 ccgcagctat acatggttga a 21 <210> 30 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_8_R <400> 30 caaacaggat ggaatcattt gaaactgatc aa 32 <210> 31 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_8_A1 <400> 31 cttcaggtgt ctcttatgta agttgc 26 <210> 32 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_8_A2 <400> 32 ccttcaggtg tctcttatgt aagttgt 27 <210> 33 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_9_F <400> 33 agcatgggat gagatagatc caa 23 <210> 34 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_9_R <400> 34 cttggaggct gcaatctgct 20 <210> 35 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_9_A1 <400> 35 ccaaaatcat caggagaagg atacaca 27 <210> 36 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_9_A2 <400> 36 ccaaaatcat caggagaagg atacact 27 <210> 37 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_10_F <400> 37 cacaacggat aacaaaagaa agagga 26 <210> 38 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_10_R <400> 38 gatgatctgt acacgcaaat aaataaactc aca 33 <210> 39 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_10_A1 <400> 39 aaccgcaatt cctgagcttc ta 22 <210> 40 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_10_A2 <400> 40 ccgcaattcc tgagcttctg 20 <210> 41 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_11_F <400> 41 tgttagaaca tgttttgacc agca 24 <210> 42 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_11_R <400> 42 ctcgttgcat tgtggacgct 20 <210> 43 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_11_A1 <400> 43 ccgtctcaaa ttatgttgga tgagag 26 <210> 44 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_11_A2 <400> 44 accgtctcaa attatgttgg atgagaa 27 <210> 45 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_12_F <400> 45 cgcagccact gcctc 15 <210> 46 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_12_R <400> 46 gcggcagggt tgcgaa 16 <210> 47 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_12_A1 <400> 47 gcctccgcct ccacg 15 <210> 48 <211> 15 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_12_A2 <400> 48 gcctccgcct ccacc 15 <210> 49 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_13_F <400> 49 gctgaagaaa gcaagcagca 20 <210> 50 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_13_R <400> 50 agtgaatcgg ctccgaagga a 21 <210> 51 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_13_A1 <400> 51 atcagcagtg cccatggat 19 <210> 52 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_13_A2 <400> 52 tcagcagtgc ccatggag 18 <210> 53 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_14_F <400> 53 cctctgtgca ataaaatcag agga 24 <210> 54 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_14_R <400> 54 gcagcactat tttgattacc tctgataatt tttcat 36 <210> 55 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_14_A1 <400> 55 acagttgaaa gaaattgtta tatccaacct tc 32 <210> 56 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_14_A2 <400> 56 gacagttgaa agaaattgtt atatccaacc tta 33 <210> 57 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_15_F <400> 57 tgacttccct tccacggtaa 20 <210> 58 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_15_R <400> 58 cccgaacaga gctacctagc a 21 <210> 59 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_15_A1 <400> 59 aggtcacact gcgctgaa 18 <210> 60 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_15_A2 <400> 60 aggtcacact gcgctgac 18 <210> 61 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_16_F <400> 61 ctggctcagt ttaattcatt gcac 24 <210> 62 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_16_R <400> 62 ccccagggca taatcctgag a 21 <210> 63 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_16_A1 <400> 63 tgattcttta aagctttact gtctcttttg aataaa 36 <210> 64 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_16_A2 <400> 64 tgattcttta aagctttact gtctcttttg aataat 36 <210> 65 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_17_F <400> 65 acaagcattg aagagtcttc ttcag 25 <210> 66 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_17_R <400> 66 ggtcaaataa tagcagctat ggttttagct c 31 <210> 67 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_17_A1 <400> 67 tttgatggcc tttcatgagc ac 22 <210> 68 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_17_A2 <400> 68 gtttgatggc ctttcatgag cag 23 <210> 69 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_18_F <400> 69 cagataatag agcatggtgc aca 23 <210> 70 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_18_R <400> 70 agcagcgggt tacggga 17 <210> 71 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_18_A1 <400> 71 accatatgcc tcaatcgtat ataggac 27 <210> 72 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_18_A2 <400> 72 caccatatgc ctcaatcgta tataggat 28 <210> 73 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_19_F <400> 73 catcaacaag attcaggaat ccca 24 <210> 74 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_19_R <400> 74 cccacactgc agctagctag t 21 <210> 75 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_19_A1 <400> 75 cccactagca ggaatcctaa ttcc 24 <210> 76 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_19_A2 <400> 76 cccactagca ggaatcctaa ttca 24 <210> 77 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_20_F <400> 77 aacttacgcg atcccctct 19 <210> 78 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_20_R <400> 78 cacgggcagc attcaactga 20 <210> 79 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_20_A1 <400> 79 cctctgcaga accaattgca atg 23 <210> 80 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_20_A2 <400> 80 cctctgcaga accaattgca ata 23 <210> 81 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_21_F <400> 81 gttgcaagca cagtgatgaa ag 22 <210> 82 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_21_R <400> 82 cagaagccac cttgttcccc 20 <210> 83 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_21_A1 <400> 83 ggtattgcag cgtttacacc a 21 <210> 84 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_21_A2 <400> 84 ggtattgcag cgtttacacc t 21 <210> 85 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_22_F <400> 85 aaccatcact acaataagca tggg 24 <210> 86 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_22_R <400> 86 cgacgaggca gcagactgta 20 <210> 87 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_22_A1 <400> 87 tgggtataac acacaatggg aataca 26 <210> 88 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_22_A2 <400> 88 tgggtataac acacaatggg aatacg 26 <210> 89 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_23_F <400> 89 gagcatggct gcgtatctg 19 <210> 90 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_23_R <400> 90 gtttgatcac tgcacgtctc catt 24 <210> 91 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_23_A1 <400> 91 gcaaatatta caaatatgtg ggtcatcctt t 31 <210> 92 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_23_A2 <400> 92 gcaaatatta caaatatgtg ggtcatcctt g 31 <210> 93 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_24_F <400> 93 gcgatttgcc tgctgcg 17 <210> 94 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_24_R <400> 94 agccatggag ctgagactga a 21 <210> 95 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_24_A1 <400> 95 ggtgaggacg tttgatccca c 21 <210> 96 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_24_A2 <400> 96 ggtgaggacg tttgatccca g 21 <210> 97 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_25_F <400> 97 agcacatccg agatcagaaa tttt 24 <210> 98 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_25_R <400> 98 tgcagcgtgt aatacttgct tttgt 25 <210> 99 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_25_A1 <400> 99 tggaacgtgc caaggcttat ataa 24 <210> 100 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_25_A2 <400> 100 tggaacgtgc caaggcttat atac 24 <210> 101 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_26_F <400> 101 acacatggca gcatgtttac g 21 <210> 102 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_26_R <400> 102 tggcacattc ccagtactgg t 21 <210> 103 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_26_A1 <400> 103 gatgtggatt gtcactttca attctct 27 <210> 104 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_26_A2 <400> 104 gatgtggatt gtcactttca attctcc 27 <210> 105 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_27_F <400> 105 gaatccggag aattatctgt ggc 23 <210> 106 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_27_R <400> 106 tcccacccga gtttcatacc c 21 <210> 107 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_27_A1 <400> 107 agctttgatt acccgacgga c 21 <210> 108 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_27_A2 <400> 108 agctttgatt acccgacgga t 21 <210> 109 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_28_F <400> 109 tccctcgttc ggccatg 17 <210> 110 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_28_R <400> 110 cagccgtgtc gaacataggg 20 <210> 111 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_28_A1 <400> 111 ccaagaagct gaaacgagtc g 21 <210> 112 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_28_A2 <400> 112 gccaagaagc tgaaacgagt ca 22 <210> 113 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_29_F <400> 113 ttgcaataaa tccagagaat gggaat 26 <210> 114 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_29_R <400> 114 gccaccggct ttgttcaca 19 <210> 115 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_29_A1 <400> 115 ttcaaccaag gatattcagg tagacag 27 <210> 116 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_29_A2 <400> 116 ttcaaccaag gatattcagg tagacaa 27 <210> 117 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_30_F <400> 117 cagcaaatgg caagtgagag a 21 <210> 118 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_30_R <400> 118 tgtctttggc tcccccagt 19 <210> 119 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_30_A1 <400> 119 aggtgtgttc ttcacaagga cc 22 <210> 120 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_30_A2 <400> 120 aggtgtgttc ttcacaagga ca 22 <210> 121 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_31_F <400> 121 cgatgaacgt atgccagaat gc 22 <210> 122 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_31_R <400> 122 gacgtacagc caagcagcc 19 <210> 123 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_31_A1 <400> 123 cagttggact catccacctt gt 22 <210> 124 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_31_A2 <400> 124 agttggactc atccaccttg c 21 <210> 125 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_32_F <400> 125 ccaactcagg taaagctaca tgaa 24 <210> 126 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_32_R <400> 126 agggaatgca atcataaata aagcatgaaa ca 32 <210> 127 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_32_A1 <400> 127 aactatctcc aaccattgta ggtcttc 27 <210> 128 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_32_A2 <400> 128 actatctcca accattgtag gtcttg 26 <210> 129 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_33_F <400> 129 tctgaaggag cagcagga 18 <210> 130 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_33_R <400> 130 agcactgtgt agtggacagc a 21 <210> 131 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_33_A1 <400> 131 agagttggcc gcatttcct 19 <210> 132 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_33_A2 <400> 132 gagttggccg catttccc 18 <210> 133 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_34_F <400> 133 cctctttctg ctgctgct 18 <210> 134 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_34_R <400> 134 tgtccccgtt caataccagc t 21 <210> 135 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_34_A1 <400> 135 ctccaaccat gacaagaaag cg 22 <210> 136 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_34_A2 <400> 136 ctccaaccat gacaagaaag ca 22 <210> 137 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_35_F <400> 137 gacaaatgag tcctgatatg aacca 25 <210> 138 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_35_R <400> 138 ccggtgttgc gagaatgtgt 20 <210> 139 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_35_A1 <400> 139 cagtctttac aatgtgaccg tgaatt 26 <210> 140 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_35_A2 <400> 140 cagtctttac aatgtgaccg tgaatc 26 <210> 141 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_36_F <400> 141 gccaagaagg gtcctgc 17 <210> 142 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_36_R <400> 142 ggaatcacta taactatctc tcatattaat ggaagca 37 <210> 143 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_36_A1 <400> 143 ctcccaaagg aaagaatgca cc 22 <210> 144 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_36_A2 <400> 144 ctcccaaagg aaagaatgca ct 22 <210> 145 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_37_F <400> 145 gcaatttcac catcaagaac aactc 25 <210> 146 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_37_R <400> 146 gctgtcaaag ggtgctgct 19 <210> 147 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_37_A1 <400> 147 ccatgcctct tccaccaaga g 21 <210> 148 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_37_A2 <400> 148 ccatgcctct tccaccaaga c 21 <210> 149 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_38_F <400> 149 gaaatggatc ctttgcagaa acc 23 <210> 150 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_38_R <400> 150 aatctgaggg ccgagcca 18 <210> 151 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_38_A1 <400> 151 ccaaacagga tcgctatgcc 20 <210> 152 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_38_A2 <400> 152 cccaaacagg atcgctatgc t 21 <210> 153 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_39_F <400> 153 gcacatcgtt gcacctgt 18 <210> 154 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_39_R <400> 154 ggaatccatg cccgcgt 17 <210> 155 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_39_A1 <400> 155 ttttctcacc gggcacgat 19 <210> 156 <211> 17 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_39_A2 <400> 156 ttctcaccgg gcacgac 17 <210> 157 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_40_F <400> 157 gtctgttatt tgctttaaag tccgc 25 <210> 158 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_40_R <400> 158 ttcactattg agcagcaaat aagttaatga tttttca 37 <210> 159 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_40_A1 <400> 159 tccgcagttg tgacattata tttgc 25 <210> 160 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_40_A2 <400> 160 gtccgcagtt gtgacattat atttgt 26 <210> 161 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_41_F <400> 161 gttctcgatc ccctcgttg 19 <210> 162 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_41_R <400> 162 ccactcccat cctctggagc 20 <210> 163 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_41_A1 <400> 163 gccagcctaa agaaaacgca tc 22 <210> 164 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_41_A2 <400> 164 gccagcctaa agaaaacgca ta 22 <210> 165 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_42_F <400> 165 tgctgcctat atgatcgact gag 23 <210> 166 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_42_R <400> 166 gcactccaat ctgcaaaagc atatca 26 <210> 167 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_42_A1 <400> 167 gatgatgaaa tgactgtttc ttctctgttt 30 <210> 168 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_42_A2 <400> 168 atgatgaaat gactgtttct tctctgttg 29 <210> 169 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_43_F <400> 169 cgcagatatt gggaagctga taa 23 <210> 170 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_43_R <400> 170 acgtccattg atgaggctgg a 21 <210> 171 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_43_A1 <400> 171 acatggtaag agatgagccc g 21 <210> 172 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_43_A2 <400> 172 ataaacatgg taagagatga gccca 25 <210> 173 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_44_F <400> 173 ggcagagagc atgtgcaata ataa 24 <210> 174 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_44_R <400> 174 aggctgcaat aggacctgaa ca 22 <210> 175 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_44_A1 <400> 175 ggtttaatgt acgccccatg tgta 24 <210> 176 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_44_A2 <400> 176 gtttaatgta cgccccatgt gtt 23 <210> 177 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_45_F <400> 177 tcgatgttcc tgataacctc agtac 25 <210> 178 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_45_R <400> 178 tgcagcaaca tagacatact gaagtga 27 <210> 179 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_45_A1 <400> 179 gactggtcag ttcacctcat tatctat 27 <210> 180 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_45_A2 <400> 180 gactggtcag ttcacctcat tatctac 27 <210> 181 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_46_F <400> 181 ggcagaatgc acgtcgattt 20 <210> 182 <211> 16 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_46_R <400> 182 ggcgatcagg cgaccg 16 <210> 183 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_46_A1 <400> 183 gaagaacgcg ctcgatttcg 20 <210> 184 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_46_A2 <400> 184 tgaagaacgc gctcgatttc a 21 <210> 185 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_47_F <400> 185 ctgtgaagga acgtcgttta gtaat 25 <210> 186 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_47_R <400> 186 acgcccaccc tttctcct 18 <210> 187 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_47_A1 <400> 187 ggtttctaca atgggtactg caac 24 <210> 188 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_47_A2 <400> 188 tggtttctac aatgggtact gcaat 25 <210> 189 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_1 <400> 189 cagtaatcgg gatcaagaag tccccggtta gggacatagt t 41 <210> 190 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_2 <400> 190 ttcaccagag ttacttcctt tgctttctga caactcatta c 41 <210> 191 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_3 <400> 191 ggtgtgcgag ggcgacctga tgaatgtcgg ggctgctgcg g 41 <210> 192 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_4 <400> 192 gatgaatatg tccatggaaa acaggatcaa tatccatgtt t 41 <210> 193 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_5 <400> 193 atggcgtaaa ctcagcatgg ctacaaagag ctttttcttg a 41 <210> 194 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_6 <400> 194 tctattgcag cagccttttt aacattggag aactcggaac t 41 <210> 195 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_7 <400> 195 aagaaagcag tggaagcaat atgccccggt gttgtctcgt g 41 <210> 196 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_8 <400> 196 catttgaaac tgatcaataa acaacttaca taagagacac c 41 <210> 197 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_9 <400> 197 tcatcaggag aaggatacac tatgttattt accaaagtga a 41 <210> 198 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_10 <400> 198 accgcaattc ctgagcttct gtgtgagttt atttatttgc g 41 <210> 199 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_11 <400> 199 tcaaattatg ttggatgaga aatgatgcag cgtccacaat g 41 <210> 200 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_12 <400> 200 gtggctgtgg atgcggtggc ggtggaggcg gaggcagtgg c 41 <210> 201 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_13 <400> 201 tctgtcttaa ctgcagattc ctccatgggc actgctgatt g 41 <210> 202 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_14 <400> 202 taatttttca taagtagttg taaggttgga tataacaatt t 41 <210> 203 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_15 <400> 203 gtaaggtcac actgcgctga caaatctgag aattccccac t 41 <210> 204 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_16 <400> 204 ttactgtctc ttttgaataa tgtttggttt cataagataa a 41 <210> 205 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_17 <400> 205 gcagctatgg ttttagctct ctgctcatga aaggccatca a 41 <210> 206 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_18 <400> 206 tgcctcaatc gtatatagga taaccagaat gagatcatac a 41 <210> 207 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_19 <400> 207 actagcagga atcctaattc atagccttta aacgacttga c 41 <210> 208 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_20 <400> 208 tctgcagaac caattgcaat actcattctc cgcttccaat c 41 <210> 209 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_21 <400> 209 ggtattgcag cgtttacacc ttcgccaagg ggaacaaggt g 41 <210> 210 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_22 <400> 210 ataacacaca atgggaatac gaaaggaatt actccgtaat a 41 <210> 211 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_23 <400> 211 caaatatgtg ggtcatcctt gcatttgtac gacaaagatc a 41 <210> 212 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_24 <400> 212 ggtgaggacg tttgatccca gagagatttc tggagaattt a 41 <210> 213 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_25 <400> 213 aattaatttt gaagaaatat gtatataagc cttggcacgt t 41 <210> 214 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_26 <400> 214 ttcgtcatac catataggat ggagaattga aagtgacaat c 41 <210> 215 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_27 <400> 215 agctttgatt acccgacgga tacattgctg ccgggtatga a 41 <210> 216 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_28 <400> 216 ccaagaagct gaaacgagtc actgctcatc tcggccacga a 41 <210> 217 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_29 <400> 217 caaggatatt caggtagaca aggatcagtc agttgctcag t 41 <210> 218 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_30 <400> 218 tttggctccc ccagtttagc tgtccttgtg aagaacacac c 41 <210> 219 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_31 <400> 219 cctttgggca gtgcatcgga gcaaggtgga tgagtccaac t 41 <210> 220 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_32 <400> 220 ctccaaccat tgtaggtctt gctgtttcat gctttattta t 41 <210> 221 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_33 <400> 221 agcattgatc attaactcgt gggaaatgcg gccaactctt t 41 <210> 222 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_34 <400> 222 atctttagct tgttagatag tgctttcttg tcatggttgg a 41 <210> 223 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_35 <400> 223 tcagtttcag aggttgaagt gattcacggt cacattgtaa a 41 <210> 224 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_36 <400> 224 tcatattaat ggaagcagta agtgcattct ttcctttggg a 41 <210> 225 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_37 <400> 225 gctgctgaga actatcatcg gtcttggtgg aagaggcatg g 41 <210> 226 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_38 <400> 226 cccaaacagg atcgctatgc tctccgcact tctccccaat g 41 <210> 227 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_39 <400> 227 taccgctgca cggtggcgcc gtcgtgcccg gtgagaaaac a 41 <210> 228 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_40 <400> 228 cagttgtgac attatatttg taatgaaaaa tcattaactt a 41 <210> 229 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_41 <400> 229 ccagcctaaa gaaaacgcat agagcacaag gcttcaccga g 41 <210> 230 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_42 <400> 230 atgactgttt cttctctgtt gtgctgtgat tatattcatt c 41 <210> 231 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_43 <400> 231 acatggtaag agatgagccc aacactgcaa ccctcaacga c 41 <210> 232 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_44 <400> 232 ttaatgtacg ccccatgtgt ttgttcaggt cctattgcag c 41 <210> 233 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_45 <400> 233 gaagtgaaaa tcaatacgaa gtagataatg aggtgaactg a 41 <210> 234 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_46 <400> 234 gcgatcaggc gaccgccgcg tgaaatcgag cgcgttcttc a 41 <210> 235 <211> 41 <212> DNA <213> Artificial Sequence <220> <223> SP_SNP_47 <400> 235 ttctacaatg ggtactgcaa tggttatgca tatgaagaag a 41

Claims (5)

A primer set represented by SEQ ID NOS: 1, 2, 3, and 4; A primer set represented by SEQ ID NOS: 5, 6, 7, and 8; A primer set represented by SEQ ID NOS: 9, 10, 11, and 12; A primer set represented by SEQ ID NOs: 13, 14, 15, and 16; A primer set represented by SEQ ID NOs: 17, 18, 19, and 20; A primer set represented by SEQ ID NOS: 21, 22, 23 and 24; A primer set represented by SEQ ID NOS: 25, 26, 27, and 28; A primer set represented by SEQ ID NOs: 29, 30, 31, and 32; A primer set represented by SEQ ID NOS: 33, 34, 35, and 36; A primer set represented by SEQ ID NOS: 37, 38, 39, and 40; A primer set represented by SEQ ID NOS: 41, 42, 43, and 44; A primer set represented by SEQ ID NOS: 45, 46, 47, and 48; A primer set represented by SEQ ID NOS: 49, 50, 51, and 52; A primer set represented by SEQ ID NOS: 53, 54, 55, and 56; A primer set represented by SEQ ID NOS: 57, 58, 59, and 60; A primer set represented by SEQ ID NOS: 61, 62, 63 and 64; A primer set represented by SEQ ID NOS: 65, 66, 67, and 68; A primer set represented by SEQ ID NOS: 69, 70, 71 and 72; A primer set represented by SEQ ID NOS: 73, 74, 75, and 76; A primer set represented by SEQ ID NOS: 77, 78, 79 and 80; A primer set represented by SEQ ID NOS: 81, 82, 83 and 84; A primer set represented by SEQ ID NOS: 85, 86, 87 and 88; A primer set represented by SEQ ID NOS: 89, 90, 91, and 92; A primer set represented by SEQ ID NOS: 93, 94, 95, and 96; A primer set represented by SEQ ID NOS: 97, 98, 99 and 100; A primer set represented by SEQ ID NOS: 101, 102, 103, and 104; A primer set represented by SEQ ID NOS: 105, 106, 107, and 108; A primer set represented by SEQ ID NOS: 109, 110, 111, and 112; A primer set represented by SEQ ID NOS: 113, 114, 115, and 116; A primer set represented by SEQ ID NOS: 117, 118, 119, and 120; A primer set represented by SEQ ID NOS: 121, 122, 123, and 124; A primer set represented by SEQ ID NOS: 125, 126, 127, and 128; A primer set represented by SEQ ID NOs: 129, 130, 131, and 132; A primer set represented by SEQ ID NOS: 133, 134, 135, and 136; A primer set represented by SEQ ID NOS: 137, 138, 139, and 140; A primer set represented by SEQ ID NOS: 141, 142, 143 and 144; A primer set represented by SEQ ID NOS: 145, 146, 147, and 148; A primer set represented by SEQ ID NOs: 149, 150, 151, and 152; A primer set represented by SEQ ID NOS: 153, 154, 155, and 156; A primer set represented by SEQ ID NOS: 157, 158, 159, and 160; A primer set represented by SEQ ID NOS: 161, 162, 163, and 164; A primer set represented by SEQ ID NOS: 165, 166, 167, and 168; A primer set represented by SEQ ID NOs: 169, 170, 171, and 172; A primer set represented by SEQ ID NOS: 173, 174, 175, and 176; A primer set represented by SEQ ID NOS: 177, 178, 179, and 180; A primer set represented by SEQ ID NOS: 181, 182, 183, and 184; And a primer set represented by SEQ ID NOS: 185, 186, 187, and 188; and a primer set for discriminating 67 kinds of sweet potato varieties shown in Table 1 below;
[Table 1]

A kit for distinguishing sweet potato varieties comprising the primer set of claim 1 and a reagent for carrying out an amplification reaction.
3. The kit according to claim 2, wherein the reagent for carrying out the amplification reaction comprises a DNA polymerase, dNTPs and a buffer.
a) separating the genomic DNA from the sweet potato sample;
b) performing PCR (polymerase chain reaction) using the separated genomic DNA as a template and using the primer set of claim 1; And
and c) detecting the amplified PCR product of step b).
5. The method according to claim 4, wherein the detection of the amplification product is performed by sequencing, DNA chip, gel electrophoresis, radioactivity measurement, fluorescence measurement or phosphorescence measurement.

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