KR101934485B1 - Cosmetic composition comprising high content human stem cell conditioned media stablized by polymer - Google Patents

Abstract

The present invention relates to a cosmetic composition which is stable and has an excellent effect of improving skin condition continuously, and more specifically, contains various skin function protein mixtures such as around 150 growth factor protein ingredients including epidermal growth factors (EGF), transforming growth factors (TGF), hepatocyte growth factors (HGF), fibroblast growth factors (FGF), insulin-like growth factors (IGF) and the like, thereby polymer-emulsifying a culture solution extract of human-derived stem cells, which is excellent in terms of skin regenerating functions but difficult to be used in a cosmetic materials with high content because of discoloring and layer separation in a long term storage, by means of acrylate polymers generated by mixing acrylate/C10-30 alkyl acrylate cross polymers with acrylate/Beheneth-25 methacrylate polymers; and alleviating skin stimulation according to usage of culture solution extracts of high content human-derived stem cells. In addition, the present invention is composed by mixing four complex extracts of lotus extracts, resurrection plant extracts, Echinacea purpurea extracts and Swertia chirata extracts for promoting skin moisturizing and improving skin tones such that stability maintains for a long term and a function for skin physiology activation can be improved.

Description

Technical Field [0001] The present invention relates to a skin-improving cosmetic composition containing a polymer-emulsified mousse having a high content of a culture medium of human-derived stem cells,

The present invention relates to a skin improving cosmetic composition, and more particularly, to a skin-improving cosmetic composition, which is rich in skin proteins and is superior in skin regeneration effect, but is easily unstable due to sunlight or high temperature, The present invention relates to a method for stabilizing a cosmetic composition capable of containing a high concentration of a cosmetic composition.

Methods for stabilizing existing human stem cell culture extracts using liposomes and nanocapsules have been studied. (Registration No. 10-1321346), a cosmetic composition comprising nanofibers or nanocapsules of a fat-derived adult stem cell culture solution, and a process for producing the same (Registration Number 10-1321346), a cosmetic composition containing a low-temperature process liposome encapsulating a human stem cell culture fluid extract -1336474). In the case of stem cell-derived exosomes containing a high content of growth factors (Publication No. 10-2017-0085010), a method for optimizing the growth factor and a method for culturing stem cells within 10% It is only shown as a method for a composition for stabilizing a culture medium. In addition, a hair care and personal care composition containing an acrylic copolymer (Laid-open No. 10-2013-0102572) proposes a method for film formation and rheological gel properties, but does not include a method for stabilizing skin complex proteins. Accordingly, in the present invention, it is intended to provide a cosmetic composition for skin improvement comprising 4 or more complex extracts, which is obtained by stabilizing 50% or more of stem cell culture extract of human body to be implemented using an acrylic polymer.

Korean Patent No. 10-1321346 relates to a cosmetic containing a low-temperature process liposome encapsulating a human stem cell culture extract, wherein the cosmetic composition containing the low-temperature process liposome according to the present invention comprises lecithin and water using supercritical carbon dioxide To form a reverse micelle emulsion, followed by depressurizing the supercritical carbon dioxide to induce a liposomal suspension to contain a human stem cell culture extract solution in a low-temperature process liposome. Therefore, unlike the case where the liposome is formed at a high temperature during the production of liposomes and the active ingredient which is weak to heat is destabilized or destroyed, the liposome of low temperature can form liposome at room temperature and low temperature, And the effect of improving skin wrinkles is excellent.

Korean Patent No. 10-1336474 relates to a cosmetic composition comprising nanofibers or nanocapsules of an adipose-derived adult stem cell culture liquid and a method for producing the same, and more particularly, to a cosmetic composition comprising a nanofiber or nanocapsule of an adipose- And a method for producing the same.

Korean Registered Patent No. 10-1321346 (Registered Oct. 17, 2013) Korean Registered Patent No. 10-1336474 (Registered on November 27, 2013)

One embodiment of the present invention is to provide a cosmetic composition capable of solving the problems of the prior art which is unstable to use more than 50% of the human stem cell culture extract rich in skin proteins.

One embodiment of the present invention is to stabilize and stabilize an acrylate / C10-30 alkyl acrylate crosspolymer and an acrylate / beheneth-25 methacrylate polymer containing an extract of human stem cell culture fluids up to 90% To provide a cosmetic composition comprising a polymeric emulsified moss as much as possible.

One embodiment of the present invention is to provide a skin improving cosmetic composition containing four complex extracts such as a lotus flower extract, an Echinochloa extract, an Aspergillus niger extract, and a Kiraya extract as a polymer emulsion moss.

Among the examples, the cosmetic composition which can prevent discoloration and phase separation during long-term preservation and improve the skin improving ability while lowering the skin irritation degree is a complex extract, a human stem mixed with the complex extract and stabilized with an acrylic polymer Cell culture broth extract.

The acrylic polymer may be prepared by mixing an acrylate / C10-30 alkyl acrylate crosspolymer and an acrylate / beheneth-25 methacrylate polymer.

The acrylate / C10-30 alkyl acrylate crosspolymer and the acrylate / beheneth-25 methacrylate polymer are constituted at a ratio of 1.0 to 2.0 times and include 0.1 to 0.5% by weight based on the total weight of the cosmetic composition can do.

The complex extract is composed of a lotus extract, an extract of Echinochloa crus-galli, an extract of Aspergillus oryzae, and a extract of Kiraya, and may contain 0.01 to 2.0% by weight based on the total weight of the cosmetic composition.

The cosmetic composition may be configured to be sprayed through the mist spray-type container.

The disclosed technique may have the following effects. It is to be understood, however, that the scope of the disclosed technology is not to be construed as limited thereby, as it is not meant to imply that a particular embodiment should include all of the following effects or only the following effects.

The cosmetic composition according to an embodiment of the present invention overcomes long-term discoloration and phase separation phenomenon through the use of a polymer emulsion having a high concentration and stability of up to 90% of a human stem cell culture extract, And the like.

The cosmetic composition according to one embodiment of the present invention can provide a skin improving cosmetic composition containing a complex extract so as to minimize skin irritation caused by human body stem cell culture extract containing high concentration and to maintain skin moisturization and skin tone .

The description of the present invention is merely an example for structural or functional explanation, and the scope of the present invention should not be construed as being limited by the embodiments described in the text. That is, the embodiments are to be construed as being variously embodied and having various forms, so that the scope of the present invention should be understood to include equivalents capable of realizing technical ideas. Also, the purpose or effect of the present invention should not be construed as limiting the scope of the present invention, since it does not mean that a specific embodiment should include all or only such effect.

Meanwhile, the meaning of the terms described in the present application should be understood as follows.

The terms "first "," second ", and the like are intended to distinguish one element from another, and the scope of the right should not be limited by these terms. For example, the first component may be referred to as a second component, and similarly, the second component may also be referred to as a first component.

It is to be understood that when an element is referred to as being "connected" to another element, it may be directly connected to the other element, but there may be other elements in between. On the other hand, when an element is referred to as being "directly connected" to another element, it should be understood that there are no other elements in between. On the other hand, other expressions that describe the relationship between components, such as "between" and "between" or "neighboring to" and "directly adjacent to" should be interpreted as well.

It is to be understood that the singular " include " or "have" are to be construed as including the stated feature, number, step, operation, It is to be understood that the combination is intended to specify that it does not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

In each step, the identification code (e.g., a, b, c, etc.) is used for convenience of explanation, the identification code does not describe the order of each step, Unless otherwise stated, it may occur differently from the stated order. That is, each step may occur in the same order as described, may be performed substantially concurrently, or may be performed in reverse order.

All terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, unless otherwise defined. Commonly used predefined terms should be interpreted to be consistent with the meanings in the context of the related art and can not be interpreted as having ideal or overly formal meaning unless explicitly defined in the present application.

Hereinafter, the configuration of the present invention will be described in detail.

One. Human origin  Stem cell culture fluid extract

The stem cell culture extract of human body used in the present invention means a stem cell obtained from an adult other than embryonic stem cells, and examples thereof include stem cells originating from the base of blood and tissues including fat, bone marrow, umbilical cord blood and peripheral blood, , But are not limited thereto. In the field of cosmetics, human-derived stem cells use growth factors as their metabolites. Growth factors bind to receptors on the surface of cells to stimulate cell proliferation and differentiation. EGF Growth factor), TGF (transforming growth factor), HGF (hepatocyte growth factor), FGF (fibroblast growth factor), IGF (insulin like growth factor), SOD (antioxidant enzyme), collagen and fibronectin .

In the present invention, the content of the human-derived stem cell culture fluid extract may be up to 90.0% by weight based on the total weight of the polymer emulsion.

2. Acrylic Polymer

 In the present invention, two kinds of acrylate / C10-30 alkyl acrylate crosspolymer and acrylate / beheneth-25 methacrylate polymer which can be used as an acrylic polymer are contained in an amount of 0.01 to 1.0% by weight based on the total weight of the polymer emulsion, Preferably 0.1 to 0.5% by weight based on the total weight of the composition, and the ratio of the acrylate / beheneth-25 methacrylate polymer to the acrylate / C10-30 alkyl acrylate crosspolymer is 1.0 to 2.0 times. When the content of the acryl-based polymer is less than 0.01 wt%, the effect is hardly exhibited. When the content is more than 1.0 wt%, 90 wt% of the stem cell culture extract of the human body is difficult to disperse and dissolve in the polymer, and the acrylate / C10-30 alkyl acrylate crosspolymer When the ratio of the contrast acrylate / beheneth-25 methacrylate polymer was lower than 1.0 times, it was difficult to improve the stability, and when it was 2.0 times or more, it was difficult to form a sprayable low viscosity emulsion.

3. Skin improvement complex extract

In the present invention, the lotus extract used for promoting the skin improvement effect contains 1.0 ~ 10.0 wt% of Leafresh (Naturalsolution) extracted from the lotus leaf native to Jeju Island, based on the total weight of the skin improvement complex extract, The extract contains 1.0 to 10.0% by weight of C-MO 2G (ToyoSugarRefining) containing glyceryl glucoside in an amount of 1.0 to 10.0% by weight based on the total weight of the skin-improving complex extract, Symflow 1298 (Symrise), which contains more than 3% of chicory acid in flowers, is contained in 0.01-1.0% by weight of the total weight of complex skin extracts. Kira Yata extract has a tradition of being used in the Himalayan region 0.1 to 5.0% by weight of SWT-7 (LucasMeyer, manufactured by Sumitomo Pharmaceutical Co., Ltd.) containing 1.25% or more of herbal medicine ingredient, The. The content of the skin-improving complex extract used in the polymer emulsion moss is 0.001 to 10.0% by weight, preferably 0.01 to 2.0% by weight, based on the total weight of the composition. If the content of the skin-improving complex extract is less than 0.001% by weight, the effect is hardly exhibited. If the content is more than 10.0% by weight, the stability of the composition may be greatly affected.

4. Other ingredients

The components included in the polymeric emulsion of the present invention include components commonly used in cosmetic compositions, such as pH adjusters, surfactants, oils, alcohols, moisturizers, antioxidants, But are not limited to, preservatives, chelating agents, flavors, pigments, ultraviolet absorbers, ultraviolet scattering agents, antioxidants, and the like.

5. Cosmetics  Formulation

The cosmetic composition containing the polymeric emulsified mousse that stabilizes the stem cell culture extract of human body derived from the human body of the present invention is not particularly limited in its formulation. Examples thereof include softening longevity, convergent lotion, nutritional lotion, eye cream, It can have formulations such as massage cream, cleansing cream, cleansing foam, cleansing water, powder, ampoule, essence, babe cream, sun cream, pack. In addition, in the cosmetic composition of each formulation, the polymer emulsion which stabilizes the human stem cell culture fluid extract can be selected and mixed without difficulty by the person skilled in the art according to the purpose without any difficulty.

The present invention will be described in more detail with reference to the following examples and experimental examples, but the present invention is not intended to be limited thereto.

( Experimental Example  One) Human origin  Stem cell culture extract-containing Polymeric emulsion  Produce

The B-phase containing the human stem cell culture extract was mixed with the homogenously mixed A-phase at room temperature, the polymer as stabilizer was added, and the mixture was thoroughly stirred. Then, the mixture was passed through a high-pressure emulsification homogenizer (Microfludizer) I got oil painting.

(Experimental Example 2)  Preparation of Liposome Containing Extract of Human Body-Derived Stem Cell Culture Solution

The B phase containing the human stem cell culture extract and the A phase were mixed homogeneously and the mixture was heated to 60 ° C to dissolve the C phase and stirred. The mixture was passed through a high-pressure emulsification microfludizer under a pressure of 1,000 bar twice to obtain a liposome .

( Experimental Example  3) Polymer oil emulsion  And Liposomes  Contained Cosmetics  Preparation of composition

Using the polymeric emulsion mousses prepared in Example 1 and the liposomes prepared in Comparative Example 6, the cosmetic materials shown in Table 3 below were mixed at room temperature with A and C phases uniformly mixed with B Phase.

( Experimental Example  4) By temperature condition Phase stability  exam

The polymeric jars, liposomes and cosmetic compositions containing them were stored at room temperature, 45 ° C, and 4 ° C, respectively. 1 day, 1 month, 2 months, 3 months, color change and phase separation were visually measured and confirmed.

(Stability evaluation standard)

A: No change,

○: No separation, but light yellow change,

▲: Very little separation and light yellow change

★: little separation and yellow change,

★★: Severe separation and dark yellow change

As shown in Table 4, when a high-content stem cell culture extract having a high content of 90% was prepared by mixing an acrylate / C10-30 alkyl acrylate crosspolymer and an acrylate / beheneth-25 methacrylate polymer, , But when the acrylic polymer was not used, discoloration and separation phenomenon appeared at 45 ° C, and in the case of ordinary liposome, discoloration and separation phenomenon were observed at room temperature and 45 ° C . Especially, as a general liposome, lecithin stabilizer does not play a role properly to stabilize a high content of 90% of human stem cell culture extract.

<Figure 1> Stability change after 3 months at 45 ℃

( Experimental Example  5) mist  Pump and With an electric spray gun  Ease of spraying test

The polymer jasmine obtained above, the liposome and the cosmetic containing the same were placed in a mist pump and an electric spray gun container, respectively, and the degree of viscous injection was confirmed.

(Evaluation criteria for ease of spraying)

◎: Evenly spraying more than 90 degrees without clogging,

▲: Occurs a little clogging and uneven distribution within 30 ~ 90 degrees,

★: Occurrence of blockage phenomenon and uneven injection within 30 degree

As shown in Table 5, when the ratio of the acrylate / beheneth-25 methacrylate polymer to the acrylate / C10-30 alkyl acrylate crosspolymer was lower than 1.0 times or 2.0 It is difficult to form a sprayable low viscosity emulsion as well as to maintain stability.

<Figure 2> Example 1. Wide spray angle in mist container

( Experimental Example  6) Skin safety test

Skin safety tests were conducted on the cosmetic compositions of Example 2, Comparative Examples 9 and 10.

A closed patch test was performed on the upper limb of 20 subjects. After applying the patch for 24 hours and removing the patch, mark the test area with Marking Pen and observe the test area after 24 hours and 48 hours, respectively. The determination was carried out 24 hours and 48 hours after the removal of the patch, and the skin reaction was judged according to the rules of the International Contact Dermatitis Research Group (ICDRG) in Table 6 below. The test results are shown in Table 7 below.

As a result of the skin safety test in Table 7, it can be seen that the cosmetic composition of Example 2 according to the present invention is a safe cosmetic without skin irritation.

(Experimental Example 7) Skin moisturization improvement test

The skin moisturization improvement test was carried out on the cosmetic composition of Example 2 and Comparative Example 9. [

The average amount of moisturizing water was measured three times with Corneometer CM825 (Courage and Khazaka, Germany) before the use of cosmetics and after 1 week of use. If the amount of moisture is high, the measured value is high; if it is low, the measured value is low. The skin moisturization improvement rate was calculated using the following formula.

<Figure 3> Corneometer

As a result of the skin moisturization-improving test in Table 8, it can be seen that the cosmetic composition of Example 2 according to the present invention is an excellent cosmetic material that helps improve skin moisturization.

( Experimental Example  8) Skin brightness improvement test

The skin brightness improvement test was performed on the cosmetic composition of Example 2 and Comparative Example 9. [

The skin brightness of the test area (ball area) was measured three times using Chromameter CR-400 before the use of cosmetics and after 1 week of use.

Chromameter is widely used to measure skin color by converting human-recognized colors into digital codes consisting of three factors. L *, a *, b * value are the following parameters respectively.

L *: luminosity,

a *: green-to-red spectrum,

b *: blue-to-yellow spectrum

Where a * and b * are the chrominance parameters and L * is the luminance parameter.

The skin brightness improvement rate was calculated using the following formula.

<Figure 4> Chromameter CR-400

As a result of the skin brightness improvement test in Table 9, it can be understood that the cosmetic composition of Example 2 according to the present invention is an excellent cosmetic composition that helps improve skin brightness.

Hereinafter, a cosmetic comprising a polymer-emulsified mousse stabilized in the culture solution of human-derived stem cell culture according to the present invention is prepared and presented. However, the composition of the present invention is not intended to be limited to the following formulation examples.

( Manufacturing example  One)  Flexible longevity (skin lotion)

The composition ingredients described in the following table are prepared according to a conventional preparation method.

(Production Example 2)  Nutrition lotion (milk lotion)

A nutritional lotion is prepared according to a conventional preparation method with the composition components described in the following table.

( Manufacturing example  3)  Nourishing cream (concentrated essence)

A nutritional cream (concentrated essence) is prepared according to a conventional manufacturing method with the composition components shown in the following table.

( Manufacturing example  4)  Massage cream

Massage creams are prepared according to the conventional preparation methods with respect to the composition components shown in the following table.

( Manufacturing example  5) ample  Mask Pack

The composition components described in the following table are prepared according to a conventional manufacturing method.

Conventional methods such as liposomes for stabilizing stem cell culture extracts of human body are required to develop a special process to inhibit the denaturation of skin function protein and can not use a high content. In addition, conventional methods using a polymer such as cellulose, xanthan gum, or carbomer can not form a stable emulsion, and there is a problem that it is difficult to produce a low-viscosity emulsion due to the formation of uneven point hardness.

The cosmetic composition of the present invention is characterized in that the polymer is emulsified using an acrylate / C10-30 alkyl acrylate crosspolymer and an acryl-based polymer as an acrylate / beheneth-25 methacrylate polymer, and a lotus flower extract Chrysanthemum extract, chrysanthemum extract, chrysanthemum extract, and kelaya extract. Accordingly, it is possible to overcome the problem of change in the phase stability over time such as discoloration, detachment and layer separation which may be present during the manufacturing process or during storage of the product, and the skin function such as lowering of skin irritation such as trouble, It is possible to have an effect that can be improved.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims It can be understood that

Claims (5)

A complex extract comprising a lotus flower extract, an extract of Echinochloa crus-galli, an extract from a thousand genera and a Kiraya extract, and containing 0.01 to 2.0% by weight based on the total weight of the cosmetic composition; And
A cosmetic composition comprising a stem cell culture extract obtained from a human body and stabilized with an acrylic polymer mixed with an acrylate / C10-30 alkyl acrylate cross polymer and an acrylate / beheneth-25 methacrylate polymer mixed with the above complex extract . delete The method of claim 1, wherein the acrylate / C10-30 alkyl acrylate crosspolymer and the acrylate / beheneth-25 methacrylate polymer are
1: 2, and 0.1 to 0.5% by weight based on the total weight of the cosmetic composition. delete The cosmetic composition according to claim 1, wherein the cosmetic composition comprises
Wherein the spray nozzle is configured to be sprayed through the mist spray-type container.