KR101885466B1 - Natural pesticide composition comprising extract of Phryma leptostachya var. asiatica Hara as an effective component - Google Patents

Abstract

The present invention relates to a natural insecticidal composition containing Phryma leptostachya extract as an active ingredient, wherein the Phryma leptostachya extract includes Leptostachyol acetate. The natural insecticidal composition of the present invention uses a material derived from a natural plant ingredient and avoids the use of a synthetic chemical substance such as an insecticidal agent to secure safety against human body and ecosystem, is excellent in economic efficiency since Phryma leptostachya is used as the ingredient, which can easily be procured as raw materials from domestic wild plants, and can provide excellent insecticidal power against Aedes aegypti and Aedes albopictus which carry dengue fever and Zika virus.

Description

[0001] The present invention relates to a natural insecticidal composition comprising an extract of Paralichthys olivaceus as an active ingredient. asiatica Hara as an effective component}

The present invention relates to a natural insecticidal composition containing an alopecia extract as an active ingredient, and more particularly, to a natural insecticidal composition which is eco-friendly and contains an insecticidal effect by containing an alopecia extract as an active ingredient.

There are more than 3,000 species of mosquito species in the world. Among them, about 500 kinds of mosquito mosquito (Aedes), 300 kinds of mosquito (Culex) and 350 kinds of malaria mosquito (Anpheles) are known to be harmful to human being. There are 20 kinds of mosquito (Aedes), 18 kinds of Culex (mosquito), and 7 kinds of Malaria mosquito (Anpheles).

Mosquitoes not only feed on people and livestock but also cause many people to lose their lives by mediating various diseases such as malaria, encephalitis, yellow fever and dengue fever and Zicca virus, and today, two thirds of the world's population is exposed to mosquito-borne diseases . In addition, the blood vomiting of the mosquito acts as a source of stress such as disturbance of sleep and restriction of outdoor activities, and it is a pest which causes serious damage directly or indirectly.

Among the mosquitoes known in the country, Chinese Anopheles sinensis has been reported to cause malaria and inland Brugia onchocerciasis ( Inland brugian filariasis ), Togo forests [ Oclerotatus togoi ( Aedes togoi)] is the red house mosquito belonging to the southern islands of onchocerciasis, the red house mosquito (Culex tritaeniorhynchus) is mediated Japanese encephalitis, and those outside the house mosquito (Culex pipiens pallens ) and underground mosquitoes ( Culex pipiens molestus ) have recently been infested in the apartment complex, causing the problem of blood collection.

All living organisms, including mosquitoes, receive energy or substances from the outside environment and exert various physiological and biochemical functions to adapt themselves to changes in the environment while maintaining the homeostasis of the living organism, and to preserve life and preserve species. The physiological biochemical function of an organism consists of compounds of various structures and their interactions and reactions. Pesticides directly or indirectly participate in the physiological and biochemical processes of these organisms and break down their normal action to kill or kill insects. Insecticides are largely involved in the nervous system of insects, involvement in energy metabolism, involvement in the regulation of growth, destruction of insect cells, and cooperative activities that enhance the effectiveness of insecticides, not direct insecticidal action. Can be classified.

Typical examples of the mechanisms of the conventional insecticides acting on the nervous system are pyrocylid pesticides such as DDT-type organochlorine insecticides and allethrin, which is an effective ingredient of mosquito-borne insecticides, Abnormal pathways are created by causing anomalies in the channel (sodium channel) and excessive nerve stimulation is transmitted, causing cramps and paralysis, killing pests.

However, these synthetic chemical insecticides increase resistance to pests, cause environmental pollution, and have negative effects on other organisms that are not subject to pesticides. Organophosphorus and carbamate compounds, which exhibit the inhibitory action of acetylcholinesterase acting on the nervous system of insects, penetrate not only into the skin of insects but also to the skin of human body and attach to the surface of the central nervous system, And the like.

Korean Patent Application No. 10-2013-0043141 discloses a composition of an insecticide comprising a plant component, which comprises a plant extract, which is a herbicide, but also contains piperonyl butoxide as an insecticidal agent And the piperonyl butoxy group itself does not exhibit an insecticidal effect, but it is necessary to avoid its use because there is a possibility of causing headache, dizziness, allergies and birth defects.

The growing need for environmentally safe, targeted, and naturally degradable insecticides is increasing with these problems. In addition, the tendency to avoid synthetic chemicals has become more pronounced due to the toxicity of MIT (methylisothiazolinon) and PHMG (polyhexamethylene guanidine), which are synthetic chemicals contained in humidifier disinfectants.

The present inventors have found that in order to meet the social demands for environmentally friendly insecticides by providing natural insecticidal compositions excellent in safety against human and ecosystem by using materials derived from natural plant ingredients and by avoiding the use of synthetic chemicals such as insecticide Thereby completing the invention.

The object of the present invention is to provide a natural insecticidal composition which uses materials derived from natural plant ingredients and is superior in safety to humans and ecosystems by avoiding the use of synthetic chemicals such as insecticidal agents.

Another object to be solved by the present invention is to provide a natural insecticide composition having excellent economic efficiency by using paraffin which is easy to procure raw materials as domestic native plants.

Another object to be solved by the present invention is to provide a natural insecticidal composition having excellent insecticidal activity against dengue fever, Egyptian house mosquito which mediates Zicavirus, and White mosquito mosquito.

One aspect of the present invention relates to a natural insecticidal composition comprising an alopecia areata extract as an active ingredient and the alopussia larvalis extract comprises Leptostachyol acetate represented by the following formula (1).

[Chemical Formula 1]

In addition, the Lycopersicon esculentum extract may be obtained by extracting a selected part from the roots, stems and leaves of an alopecia globule with an organic solvent and extracting the dry weight ratio of roots, stems and leaves to a range of 1: 0.8-1.2: 0.1-3.

The lupine extract may further comprise a lignan compound represented by the following general formulas (2) to (4);

(2)

(3)

[Chemical Formula 4]

The lupine extract may further comprise a lignan compound represented by the following general formula (5).

 [Chemical Formula 5]

In Formula 5, R is an alkyl group having 1 to 5 carbon atoms.

In addition, the Paraliptera extract may be prepared by mixing dry parmesan and water as an extraction solvent at a weight ratio (w / w) of 1: 2 to 1:10 and then extracting for one week at room temperature (25 ° C) , Ethyl acetate and dichloromethane is used as an extraction solvent, and the dry paraffin and the organic solvent are mixed at a weight ratio (w / w) of 1: 2 to 1:10, For 3 hours.

In addition, the natural insecticidal composition may include the Paraliptera extract and ethanol, and the Paraliptera extract may be contained at a concentration of 100 ppm to 220 ppm.

In addition, the natural insecticidal composition may have an insecticidal potency of 97% or more against the mosquito borne which mediates dengue fever and zykova virus.

INDUSTRIAL APPLICABILITY The natural insecticidal composition of the present invention uses materials derived from natural plant ingredients and is superior in safety against human and ecosystem by avoiding the use of synthetic chemicals such as insecticide and the like, It has an excellent insecticidal effect against dengue fever, Egyptian mosquito that mediates Zicca virus, and white mosquito mosquito.

Fig. 1 is a photograph of a parrot in the wild.
Fig. 2 shows the results of an analysis of Paraliphus Extract extracted from the rootstock of Example 1 by LC-MS chromatogram. Fig.

Hereinafter, embodiments of the present invention will be described in detail. However, it should be understood that the present invention is not limited thereto, and the present invention is only defined by the scope of the following claims.

The present invention relates to the insecticidal use of Paraliptera extract, and is characterized in that it provides a natural insecticidal composition containing Paraliptera extract as an active ingredient.

Fig. 1 is a photograph of a parrot in the wild. ' Phryma leptostachya var. Asiatica Hara' is a perennial herb which is a perennial herb that lives in the mountains of each country. The growth environment is growing in fertile soil with semi-fine or light soil, about 70㎝ in height, 7 ~ 9㎝ in length, 4 ~ 7㎝ in width, long petiole and hairy on both sides, It is wide oval and facing. It is a poisonous plant. It feeds the juice of roots to paper and kills flies, so it is called paraplor. It has the effect of decrypting if it sticks to the bark, bark, insect bite, etc. It is distributed in Korea, Japan, China, Himalaya Mountains, East Siberia.

In the present invention, the Paraliptera extract is prepared by extracting a selected part from the roots, stems and leaves of Paraliptera with an organic solvent.

The extraction may be carried out in an organic solvent such that the dry weight ratio of the root: stem: leaf is 1: 0.8 to 1.2: 0.1 to 3, more preferably 1: 0.8 to 1.2: 0.1 to 0.5. Exhibits an excellent insecticidal effect in the weight ratio range, and at the same time, exhibits a harmless effect on the human body.

The extraction solvent may be distilled water, a lower alcohol such as ethanol, methanol, butanol, acetone, ethyl acetate, dichloromethane, or an intermediate polar organic solvent. The extraction solvent may be used alone or in combination of two or more. Particularly, Is more preferable.

Specifically, the Paralipteroptera extract is prepared by mixing dry parmesan and water as an extraction solvent at a weight ratio (w / w) of 1: 2 to 1:10 and then extracting at room temperature (25 ° C) for one week, The dried alumina and organic solvent were mixed at a weight ratio (w / w) of 1: 2 to 1:10 by using a lower alcohol such as acetone, ethyl acetate, dichloromethane or an intermediate polar organic solvent as an extraction solvent, It can be extracted for 3 hours.

In addition, it is possible to produce a Paraliptera extract by a conventional extraction method such as a low temperature extraction method, a room temperature extraction method, an ultrasonic extraction method, or a reflux extraction method. The obtained extract is filtered with a filter paper, and the resulting filtrate is subjected to freeze drying, And can be extracted.

The Lycopersicum extract thus extracted can be isolated in high purity through various conventional separation methods as necessary. Separation methods such as solvent distribution, precipitation separation, recrystallization, and column chromatography can be used as separation methods usable at this time.

In one embodiment, the natural insecticidal composition containing the above-mentioned Paraliptera extract as an active ingredient comprises Leptostachyol acetate represented by the following formula (1).

[Chemical Formula 1]

In another embodiment, the natural insecticide composition may comprise a lignan compound represented by the following general formulas (2) to (4).

 (2)

(3)

[Chemical Formula 4]

Wherein the lignan compound represented by Formula 2 is Phrymarolin B, the lignan compound represented by Formula 3 is Phrymarolin I, and the lignan compound represented by Formula 4 is selected from Haedoxane A).

In one embodiment, the natural insecticidal composition of the present invention may contain the lignan compound represented by Formula 2 or Formula 3 and the lignan compound represented by Formula 4 in a molar ratio of 2: 1 to 4: 1. It is possible to exhibit an excellent insecticidal effect in the above-mentioned molar ratio range.

In another embodiment of the present invention, the lignan compound represented by Formula 3 may be reacted with the reaction mechanism of Reaction Scheme 1 to produce a trazole derivative of a primerolin compound.

[Chemical reaction formula 1]

In one embodiment, the natural insecticidal composition of the present invention may comprise a triazole derivative of a primarolone compound represented by the following formula (5).

[Chemical Formula 5]

In Formula 5, R is an alkyl group having 1 to 5 carbon atoms.

In one embodiment, the natural insecticidal composition of the present invention comprises a Paralytic extract and ethanol, and the Paralytic extract may be included at a concentration of 100 ppm to 220 ppm. It exhibits excellent insecticidal power in the above concentration range and is extremely small in toxicity and is safe for human body and ecosystem.

The natural insecticidal composition of the present invention can be prepared and used in various formulations such as tablets, liquid powders, mist, aerosols, emulsions, fumigant fillers, spray aerosols and the like.

The above-described natural insecticidal composition of the present invention uses materials derived from natural plant ingredients and is superior in safety against human and ecosystem by avoiding the use of synthetic chemicals such as insecticide and the like, and is easy to mass-produce into domestic native plants The use of a flapper as a material provides an economical effect.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to embodiments of the present invention. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited to the following examples. The contents not described here are sufficiently technically inferior to those skilled in the art, and a description thereof will be omitted.

Example  1-2: Preparation of natural insecticidal compositions

Example  One

A method for producing a natural insecticidal composition containing alopecia as an active ingredient is as follows. The rootstock was cut and dried in the shade for 48 hours. Three droppings and methanol as an extraction solvent were added to the extractor at a weight ratio of 3: 7, and the mixture was extracted at room temperature (25 ° C) for one week, filtered, and concentrated under reduced pressure to obtain parchment extract.

The Paraliptera extract was diluted with ethanol to make a 1% (10,000 ppm) standard product. At this time, the amount of the standard product was made to be 50 ml, and sealed in a refrigerator so that the ethanol did not evaporate. 2 ml of the standard product and 18 ml of ethanol were diluted in a vortex mixer to make 0.1% (1,000 ppm), and 2 ml of the prepared product (0.1%) was dispensed. The mixture was diluted with 18 ml of ethanol to obtain 0.01% (100 ppm) Insecticidal compositions were prepared. Insecticidal activity and toxicity were evaluated according to the following methods, and the results are shown in Table 1 below.

Example  2

A natural insecticidal composition was prepared in the same manner as in Example 1, except that the concentration of Lycopene extract was 200 ppm. Insecticidal activity and toxicity were evaluated according to the following methods, and the results are shown in Table 1 below.

Insecticide  Assessment Methods

The insecticidal potency of a natural insecticidal composition containing an alopecic extract of the Aedes albopictus larvae mediating dengue fever and Zicca virus was evaluated. The insecticidal potency test was conducted by the WHO (2005) and the Disease Control Headquarters (2005) 'Experimental Guideline for Assessing the Effectiveness of Mosquito Insecticides'.

1) Discharge preparation: Twenty mosquito larvae were placed in a cup containing 20 ml of water. The temperature (27 ± 2 ℃) was maintained for the growth of the pupa. Two cups (20 cups / cup) were prepared and two cups (20 cups / cup) were prepared for the control group.

2) Exposure of insecticidal composition: 230 ml of water was put into a prepared cup. The temperature of the water was adjusted to 26 ± 2 ° C. 1 ml of the insecticidal composition prepared in each concentration cup was dropped on the surface, and the mixture was stirred for 30 seconds using a pipette, and 2 cups were prepared for each concentration. In the control group, 1 ml of ethanol was added. Within 15-30 minutes, the prepared larvae were placed in a cup containing insecticide. It was kept at room temperature 27 ± 2 ° C and humidity 75 ± 5% for 24 hours.

3) Observation: After 24 hours, the dead water of the insects was identified. When determining the mortality rate for each concentration, the dead water in the two cups and the almost non-moving number (moribund) were measured.

Toxicity assessment

Human hepatocellular carcinoma cell line HepG2 (human hepatocellular carcinoma cell line) was purchased from Korea Cell Line Bank and used for the experiment. Cultures using 10% FBS (fetal bovine serum) , 1% glutamine, penicillin (100 g / mL), streptomycin (100 g / mL) DMEM (Dulbecco's Modified Eagle's Medium) containing a culture medium 37 ℃, CO ₂ 5 % Condition of humidity. 1 × 10 ⁴ HepG2 cells were seeded in 96-well plates, and cultured in 5% CO ₂ and 37 ° C. for 24 hours. The complete medium was replaced with serum-free medium, The control group was treated without treatment, and the cell viability was measured after incubation for 4 hours.

Example 1 Example 2 Control group Mortality rate 95% 99% - Cell Viability 99% 98% 100%

Fig. 2 shows the results of an analysis of Paraliphus Extract extracted from the rootstock of Example 1 by LC-MS chromatogram. Fig. As can be seen from FIG. 2 and Table 1, it can be seen that the extract of Paraliphus used in the examples of the present invention contains Leptostachyol acetate, and it is possible to use it without using synthetic chemicals such as insecticide Dengue fever, and zykova virus. In addition, as can be seen from the evaluation of cytotoxicity, the toxicity of the Paraliptera extract is minimal and it is safe for human and ecosystem.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be understood that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. It is therefore to be understood that the embodiments described above are in all respects illustrative and not restrictive.

Claims (6)

delete Cut the apical larvae and dry them in the shade for 48 hours.
The above-mentioned three kinds of lobster and methanol as an extraction solvent are added to an extractor at a weight ratio of 3: 7, extracted at room temperature (25 ° C) for one week, filtered and concentrated under reduced pressure to obtain a lobster extract; And
And diluting said Paraliptera extract with ethanol so that said Paraliptera extract is contained at a concentration of 200 ppm,
A method for producing a natural insecticidal composition comprising paraplum as an active ingredient, characterized in that the insecticidal power against the mosquito bacillus that mediates dengue fever and Zicca virus is 97% or more. delete delete delete delete

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