KR101881016B1 - Composition for lifespan extension containing eggplant extract - Google Patents

Abstract

One embodiment of the present invention provides a composition for prolonging life comprising an eggplant extract as an active ingredient.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for prolonging life span,

The present invention relates to a composition for prolonging the life of a composition containing a branched extract as an active ingredient, and more particularly, to a composition capable of extending the life span by activating an antioxidant enzyme.

As the science and technology develops, attempts to extend the life span of human beings have been continuously carried out, and the field of life extension science has been rapidly gaining popularity.

In the past, studies related to anti-aging have been mainly focused on such life extension science, but researches for actively prolonging life such as reverse aging have been actively carried out. Sectors such as tissue regeneration, stem cells, regenerative medicine, molecular regeneration, and artificial organs fall into the science and technology field that represents this reverse aging.

Some of them are doing continuous research on the assumption that life extension can be assisted by using methods such as nutrition, skin care and hormone therapy. In this regard, there is a market of 50 billion dollars per year in the United States alone.

However, it is still unclear whether ancillary means of extending human longevity are effective, and there are ethical problems with many living things that are inevitably sacrificed in the course of research.

Recently, the University of Texas, USA, conducted a study on the efficacy of rapamycin, an antibiotic in rats, to prove that rapamycin can extend human life to a maximum of 142 years have.

Despite the efficacy of rapamycin as described above, in the experimental group administered with this drug, the length of the body and the genital length of the male decreased, and there was a serious side effect such as decreased visual acuity or diabetes, .

Thus, research on synthetic compounds that prolong the life span of humans can cause fatal side effects, and therefore there is a growing need for studies on natural products with a significantly lower probability of occurrence of such side effects.

On the other hand, the free radical theory that explains the mechanism of aging is the theory that free radicals generated in the metabolic process react with other molecules to damage the cell structure and cause aging.

According to this, human beings repeated the metabolic process such as oxidation and energy generation of nutrients from the time of birth, accumulate active oxygen in the body, and cancer and various chronic diseases are caused by the increase of cell metabolism abnormality or mutant cell as age gets older.

In addition, researches that oxidative stress caused by active oxygen causes various diseases or aging are dominant, and accordingly, antioxidant that can suppress such oxidative stress is emerging. [Prior Art Literature] - Kim Bo-kyung and two others. Cancer Prevention Research. Vol. 15, No. 1, 2010, pp. 69-75 - Kim Hyun Young and three others. CNU Journal of Agricultural Science. Vol. 38, No. 4, 2011, pp. 625-629 - KR1020150108065

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems occurring in the prior art, and it is an object of the present invention to provide a composition for prolonging the life span comprising an antioxidant derived from natural materials as an active ingredient, .

One aspect of the present invention provides a composition for prolonging life comprising an eggplant extract as an active ingredient.

In one embodiment, the concentration of the eggplant extract may be from 10 μg / mL to 300 μg / mL.

In one embodiment, the composition can enhance the activity of superoxide dismutase (SOD).

In one embodiment, the composition can increase the activity of SIRT1 (Sirtuin 1).

In one embodiment, the eggplant extract may promote cell viability or proliferation of the yeast.

In one embodiment, the extraction solvent may be water, an alcohol, or a mixture thereof.

In one embodiment, the composition may be a pharmaceutical composition or a food composition.

According to one aspect of the present invention, the use of an extract of a natural product as a food or a pharmaceutical composition can improve the effect of extending the life of the composition while minimizing side effects that may be caused by an artificial compound.

It should be understood that the effects of the present invention are not limited to the effects described above, but include all effects that can be deduced from the description of the invention or the composition of the invention set forth in the claims.

FIG. 1 is a graph showing the effect of the content of eggplant extracts on the bio-yeast growth rate according to an embodiment of the present invention.
FIG. 2 is a graph showing the effect of the content of eggplant extracts on wild yeast growth rate according to an embodiment of the present invention.
FIG. 3 is a graph showing the effect of eggplant extract and glucose content on yeast growth rate according to an embodiment of the present invention.
FIG. 4 is a graph showing the effect of the contents of eggplant extracts on antioxidant enzyme activity according to an embodiment of the present invention.
FIG. 5 is a graph showing the effect of eggplant extract according to an embodiment of the present invention on cell viability according to calorie restriction.
FIGS. 6 and 7 are graphs showing the effect of the extract of eggplant on cell viability according to UVB irradiation according to an embodiment of the present invention.
8 is a graph showing the effect of eggplant extract according to an embodiment of the present invention on the amount of reactive oxygen species produced by UVB irradiation.
9 is a graph showing the effect of eggplant extract according to an embodiment of the present invention on Sirt1 enzyme activity.
10 is a graph comparing yeast growth rates according to the treatment of eggplant extract and other plant extracts according to an embodiment of the present invention.

As used herein, the terminology used herein is intended to encompass all commonly used generic terms that may be considered while considering the functionality of the present invention, but this may vary depending upon the intent or circumstance of the skilled artisan, the emergence of new technology, and the like. Also, in certain cases, there may be a term selected arbitrarily by the applicant, in which case the meaning thereof will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term, not on the name of a simple term, but on the entire contents of the present invention.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.

The numerical range includes numerical values defined in the above range. All numerical limitations of all the maximum numerical values given throughout this specification include all lower numerical limitations as the lower numerical limitations are explicitly stated. All the minimum numerical limitations given throughout this specification include all higher numerical limitations as the higher numerical limitations are explicitly stated. All numerical limitations given throughout this specification will include any better numerical range within a broader numerical range, as narrower numerical limitations are explicitly stated.

Hereinafter, embodiments of the present invention will be described in detail, but it should be apparent that the present invention is not limited by the following examples.

The composition for prolonging the life according to one aspect of the present invention may contain an eggplant extract as an active ingredient.

This "longevity" means extending the lifetime of an individual to a longer term as compared to a natural case. For example, the "longevity" may mean extending the life span of an individual determined by inherent or acquired factors such as genetic factors. Specifically, the "extended life" may be an extension of the lifetime of the individual by appropriately controlling the factors controlling the lifespan caused by genetic factors of the individual, such as the degree of expression of the related gene and the degree of production of the related protein, Or the like, to extend the lifetime of the individual. For example, in the present specification, the "longevity" may mean delaying the natural death of an organism.

The branch ( Solanum melongena ) is a perennial herbaceous herbaceous plant of the dicotyledonous monocotyledonous plant. It is native to India and is cultivated from tropical to temperate, and its fruit is called eggplant.

These varieties are various horticultural varieties, and each varieties are cytogenetically similar, so that hybrids can be easily produced. In addition, a single hybrid is strong and resistant to diseases, but also has a high utilization rate because it has high yield and simple mating.

Branch shells contain a large amount of purple anthocyanin pigments corresponding to polyphenol compounds. The polyphenol contains two or more hydroxyl groups in the molecule and is excellent in the effect of preventing DNA and protein from being damaged by exposure to active oxygen.

In addition, the eggplant extract can increase the activity of SOD (superoxide dismutase), which is an antioxidant in the human body. The SOD functions to prevent cell and tissue damage due to exposure to active oxygen such as superoxide generated in the human body.

The SOD exists in the human body as copper / zinc SOD (copper / zinc SOD) and manganese SOD (manganes SOD), and they inhibit the damage caused by reactive oxygen species in cytoplasm and mitochondria, .

That is to say, the eggplant extract itself functions not only as an antioxidant but also increases the activity of antioxidants present in the human body, so that the efficiency can be doubled.

Unlike the artificial compound, the eggplant extract uses antioxidants derived from natural materials as it is. Therefore, it is possible to minimize the occurrence of fatal side effects due to ingestion or administration thereof.

On the other hand, the type of solvent in the extraction process is not particularly limited, and various solvents may be used as needed. In addition, the extraction can be carried out using a conventional method in the art such as cold-rolling, warming, heating, and the like using the solvent.

Accordingly, the eggplant extract can be extracted using water, alcohol or a mixture thereof as an extraction solvent, and water is preferably used as an extraction solvent, but the present invention is not limited thereto.

When water is used as the extraction solvent, not only the safety can be enhanced but also it is harmless to the human body. Therefore, it can exhibit excellent suitability for use as a composition of food or medicine, The economic effect is also excellent. Thus, the composition for prolonging the life may be a pharmaceutical composition or a food composition.

When the composition for prolonging the life span is a pharmaceutical composition, it may further comprise a carrier, an excipient and a diluent used for preparing a conventional pharmaceutical composition.

Examples of the carrier, excipient and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, But are not limited to, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate or mineral oil.

In addition, the composition may be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like, external preparation, suppository and sterilized injection solution.

The solid preparations for oral administration include tablets, pills, powders, granules, capsules and the like. These solid preparations are prepared by adding to the branch extracts and fractions thereof at least one excipient such as starch, calcium carbonate, sucrose, lactose , Or gelatin. In addition to the above excipients, lubricants such as magnesium stearate and talc may also be used.

Examples of the liquid preparation for oral administration include suspensions, solutions, emulsions, syrups, and the like. In addition to water and liquid paraffin, simple diluents, various excipients such as wetting agents, sweeteners, have.

The preparation for parenteral administration may be a sterilized aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a lyophilized preparation, or a suppository. Examples of the non-aqueous solvent and suspensions may include injectable esters such as propylene glycol, polyethylene glycol, vegetable oil such as olive oil and ethyl oleate. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, and glycerogelatin.

A pharmaceutically effective amount of the pharmaceutical composition comprising the egg branch extract may be administered to a subject. The " pharmaceutically effective amount " means an amount sufficient to treat the disease at a reasonable benefit / risk ratio applicable to the medical treatment, and the effective dosage level will depend on the type of disease, severity, activity of the drug, Sensitivity, time of administration, route of administration and rate of excretion, duration of treatment, factors including co-administered drugs, and other factors well known in the medical arts.

The pharmaceutical composition may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or in multiple doses. It is desirable to administer an amount that allows for all of the above factors to be maximally effected in a minimal amount without side effects, which can be readily determined by one skilled in the art.

When the composition for extending the life span is a food composition, the food may include meat, snacks, dairy products, and beverages, but is not limited thereto and can be understood as including all conventional health functional foods.

The health functional food refers to a food prepared and processed by using a raw material or a component having a useful function in the human body according to the Health Functional Food Act No. 6727. The functional property refers to the nutrient Or for the purpose of obtaining a beneficial effect for health use such as physiological action.

The above-mentioned health functional foods may contain conventional food additives, and the above-mentioned food additives may be added to the standards and standards of the relevant items in accordance with the General Rules and General Test Methods approved by the Food and Drug Administration, It can be judged whether it is suitable or not. The food additives described in the above-mentioned Food Additives Code include chemical compounds such as ketones, glycine, potassium citrate, nicotinic acid, and cinnamic acid, natural additives such as persimmon extract, licorice extract, crystalline cellulose, high- A preservative formulation, a tar coloring agent, and the like.

The health functional food can be used for various foods and beverages to extend life, and can be used for various foods, beverages, gums, tea, vitamin complex, health functional supplement, food additive and the like.

The health functional food may include 1.0 to 10.0% by weight of the composition for prolonging the life of the subject for the purpose of prolonging the life of the subject. If the composition for prolonging the service life is less than 1.0 wt%, the effect of extending the service life may not be sufficiently realized. If the composition is more than 10.0 wt%, the quality of the product may not be realized or cost efficiency may be deteriorated.

In addition, the health functional food may be manufactured and processed into any one form selected from the group consisting of tablets, granules, powders, capsules, liquid solutions and rings for the purpose of extending the life of the subject.

Specifically, the health functional food in the form of tablets may be prepared by granulating a mixture with a branch extract, an excipient, a binder, a disintegrant and other additives by a conventional method, and then compressing the mixture by a lubricant or the like, . In addition, the health functional food of the tablet form may contain a starch or the like as needed, and may be sieved with a suitable skin care agent if necessary.

The hard capsule of the capsule type health functional food can be prepared by filling a normal hard capsule with a mixture with an additive such as egg branch extract and an excipient, or by granulating or granulating the granular or granular material. The soft capsule is prepared by mixing eggplant extract and excipient And the like can be filled in a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The above-mentioned ring-type health functional food can be prepared by molding a mixture of eggplant extract, excipient, binder, disintegrant and the like by an appropriate method. If necessary, the preparation may be mixed with white sugar or other suitable skin care agent or a starch, talc or a suitable substance It is possible that the

The granular health functional food may be prepared into a granular form by a suitable method such as eggplant extract, excipient, binder, disintegrant and the like, and may contain a flavoring agent, a starch and the like if necessary.

The definitions of the above excipients, binders, disintegrants, lubricants, detergents, flavorings and the like may be those described in documents known in the art and include the same or similar functions.

On the other hand, the eggplant extract can be used by adjusting the concentration thereof to an appropriate range. Specifically, the concentration of the eggplant extract may be 10 μg / mL to 300 μg / mL. If the concentration is less than 10 μg / mL, the content of the branch extract contained in the composition may be too small to extend the life span. If the concentration is less than 10 μg / mL, the formulation stability may be deteriorated and the cost- Can occur.

As described above, the eggplant extract contained in the composition for prolonging the life span can realize an effect of extending the life of the subject by increasing the antioxidant activity, and in particular, it can exert an excellent effect in promoting cell viability or proliferation ability of the yeast. The type of the yeast is not particularly limited, such as wild yeast and compressed yeast.

Specifically, the yeast is selected from the group consisting of Saccharomyces , Schizosaccharomyces , Sporobolomyces , Torulopsis , Trichosporon , Wickerhamiae Wickerhamia), Ashdod Bahia (Ashbya), Blas Sat My process (Blastomyces), Candida (Candida), between the interrogating my process (Citeromyces), Crescent bromo potassium (Crebrothecium), Cryptococcus Rhodococcus (Cryptococcus), de Bari Oh, my process (Debaryomyces) , Endomycopsis , Geotrichum , Hansenula , Kloeckera , Lipomyces , Pichia , Rhodosporidium , or The yeasts of the genus Rhodotorula (Genus) can also be exemplified, but are not limited thereto.

In addition, the composition for prolonging the life span can promote the cell viability or proliferation ability of the yeast depending on its concentration in the presence of glucose. This means that as the concentration of glucose increases, cell viability or proliferation is further promoted.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, but it should be apparent that the present invention is not limited by the following embodiments.

Manufacturing example

300 g of dried eggplant powder was added to 500 mL of purified water and stirred, and then extracted three times in succession using an ultrasonic extractor at room temperature for 1 hour and 30 minutes. The extracts were filtered and lyophilized to prepare eggplant extracts.

Experimental Example 1: Evaluation of bio-yeast growth rate according to the contents of branching extracts

5 g of commercially available yeast (compressed yeast) and 10 g of sugar were added to 100 mL of the third distilled water and reacted at room temperature for 1 hour to prepare a yeast reaction product.

The yeast reactant was diluted to 1/100 and dispensed into a 24-well plate at a concentration of 2x10 ⁴ cells / well, and then the egg plant extracts of the examples were added at a concentration of 50 μg / mL and 250 μg / mL, respectively. Each of the media was incubated at 37 ° C. for 48 hours, and the absorbance of each sample was measured at 575 nm using a microplate reader. The absorbance of each sample was compared with that of the control to evaluate the yeast growth rate (FIG. 1) .

Referring to FIG. 1, the branched extract of the example showed a concentration-dependent yeast growth rate, and showed a yeast growth rate of about 130% at a concentration of 250 μg / mL compared with the control.

These results suggest that the extracts of natural plant extracts may have an excellent life - span effect while minimizing adverse effects.

Experimental Example 2: Estimation of growth rate of wild yeast according to the contents of eggplant extracts

Yeast reaction products were prepared by adding 5 g of wild yeast, 5 g of honey, 100 g of raisins, and a small amount of powder to 200 mL of the third distilled water, followed by reaction at 30 ° C for 24 hours.

The yeast reactant was diluted 1/10 and dispensed into a 24-well plate at a concentration of 2x10 ⁴ cells / well, and then the egg plant extracts of the above examples were added at concentrations of 50 μg / mL and 250 μg / mL, respectively. Each of the media was incubated at 37 ° C. for 48 hours, and the absorbance of each sample was measured at 575 nm using a microplate reader. The absorbance of each sample was compared with that of the control to evaluate the yeast growth rate (FIG. 2) .

2, the extracts of the examples showed an increase in the yeast growth rate as the concentration increased and a yeast growth rate of about 130% as compared with the control group at the concentration of 250 μg / mL. Respectively.

These results suggest that the life extension effect of the eggplant extract is not limited to yeast of a specific species.

Experimental Example 3: Evaluation of yeast growth rate according to glucose and eggplant extract contents

After adding glucose to the raw yeast medium of Experimental Example 1 at different concentrations of 0.1, 1, 5, and 10% (w / v), 50 μg / mL and 250 μg of the eggplant extracts of the above- / mL, respectively.

Thereafter, the absorbance of each medium was measured at 575 nm using a microplate reader for each medium, and the absorbance of each sample was compared with the control group to evaluate the yeast growth rate (FIG. 3).

3, it was confirmed that the eggplant extract increased the yeast growth rate depending on the glucose concentration from 2 days after the addition of the eggplant extract, and this result shows that the eggplant extract promotes yeast growth and maintains the life Suggesting that it is superior in efficacy.

Experimental Example 4: Evaluation of antioxidant enzyme activity according to the contents of eggplant extracts

The enzyme activity of Superoxide dismutase (SOD) was measured after extracting a protein sample from the medium of Experimental Example 1.

Specifically, 700 μL of a reagent containing 0.105 mM xanthine and 1.1 mM cytochrome C was mixed with 50 μL of the protein sample, and then 50 μL of 0.05unit of xanthine oxidase (XOD) was added To measure XOD inhibitory activity. SOD activity was calculated through XOD inhibitory activity (Fig. 4).

As shown in FIG. 4, the SOD activity of 250 μg / mL of the eggplant extract was higher than that of the eggplant extract of 50 μg / mL, but the yeast treated with the eggplant extract had a higher SOD Lt; / RTI >

These results suggest that eggplant extracts may increase the antioxidant enzyme activity and thus extend the life span.

Experimental Example 5: Evaluation of cell viability according to calorie restriction

And the average age of yeast was measured by growth conditions.

5, the cell survival rate was only 35% after 6 days in the control group without the eggplant extract treatment, but the cell survival rate in the group treated with eggplant extract at 100 μg / mL reached 66%, and 10 μg / mL and 50 μg / mL, respectively, were measured as 54% and 60%, respectively.

The cell survival rates of the extracts treated with different concentrations of 10, 50 and 100 μg / mL were 48%, 61% and 72%, respectively. %, And the maximum life of yeast was significantly increased by treatment with eggplant extract even under calorie restriction conditions.

Experimental Example 6: Evaluation of cell viability by UVB irradiation

The extinction effect of eggplant extracts was evaluated by measuring the intensity of absorbance at 660 nm after UVB irradiation under different glucose conditions (2, 5, 0.5%).

6, the cell survival rate of yeast was decreased by UVB irradiation, but the cell survival rate of yeast treated with eggplant extract was remarkably increased.

In addition, cell viability was measured by UVB irradiation with different concentrations of extracts (10 μg / mL, 50 μg / mL, 100 μg / mL) and with or without calorie restriction.

Referring to FIG. 7, the cell survival rate was only 3% after 6 days under the condition of no calorie restriction during UVB irradiation, but the cell survival rate increased to 22% when treated with 100 μg / mL of eggplant extract.

Also, the maximum life of yeast was significantly increased by treatment with eggplant extracts under high calorie and calorie restriction conditions.

Experimental Example 7: Evaluation of reactive oxygen species production by UVB irradiation

The total amount of ROS (peroxynitrite anion, hydrogen peroxide, and peroxyl radical) by UVB irradiation was compared using DCF-DA staining and FACS (Fluorescence activated cell sorter) analysis under different glucose conditions (2, 5, 0.5%).

Referring to FIG. 8, the amount of ROS produced by the eggplant extract treatment was remarkably decreased. These results suggest that eggplant extract may prolong the life span of yeast cells by inhibiting the production of reactive oxygen species.

Experimental Example 8: Evaluation of Sirt1 enzyme activity according to the contents of eggplant extracts

The changes of Sirt1 activity were measured by the extracts of eggplant extracts. Recently, the linkage mechanisms of cancer and senescence are known, and SIR2 gene (SIR2), a homologue of SIR1 gene, is present as a linkage in yeast.

In connection with the study of longevity genes, Guarente's team at MIT in 1999 reported that SIR2 prolongs the life span of Saccharomyces cerevisiae.

The SIRT is NAD + (nicotinamide) dependent histone deacetylase, which is associated with aging control and increased lifespan by dietary restriction. The expression of SIRT1 was increased by dietary restriction in animal models including experimental rats, and the overexpression of SIRT increased the lifespan of yeast, nymphs, and flies. When the SIRT was mutated, It did not appear. That is, the effect of prolonging the lifespan of dietary restrictions is reported to be related to SIRT, and the effect is conservative among species.

Referring to FIG. 9, the eggplant extract (10, 50, 100, 250 μg / mL) increased the enzyme activity of Sirt1 to 250 μg / mL in a concentration-dependent manner.

In addition, when the concentration of delphinidin (1, 5, 10 μM), which is one of the main compounds of the branch, was tested, it was found that Sirt1 activity increase effect was higher than resveratrol, a sirt1 activator used as a positive control However, Sirt1 activity of eggplant extract was evaluated to be superior to that of positive control.

Experimental Example 9: Comparison of yeast growth rate by sample treatment

The extracts of other plant extracts were treated and the cell growth rate of yeast was increased.

As shown in FIG. 10, when the extracts of mulberry leaves (MB), branches (EP), acai berry (AB), apple leaves (AP) And the growth of cells was increased in a concentration-dependent manner.

The above results suggest that the eggplant extract contains a bioactive compound capable of controlling the senescence of the yeast, and thus the life extension effect is excellent.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

Claims (7)

A composition for prolonging the life of a yeast, comprising an eggplant extract as an active ingredient and promoting cell viability and proliferation ability. The method according to claim 1,
Wherein the concentration of the eggplant extract is 10 占 퐂 / mL to 300 占 퐂 / mL. The method according to claim 1,
A composition for prolonging the life of a yeast that increases the activity of superoxide dismutase (SOD). delete delete delete delete

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