KR101873359B1 - Composition for controlling universal plant bacterial disease comprising natural substance as effective component and uses thereof - Google Patents

Abstract

The present invention relates to a composition for controlling a wide range of plant bacterium diseases containing natural products as an active ingredient and a use thereof and, more particularly, to a composition for controlling plant bacterium which contains a mixture of berberine and gallotannin as an active ingredient . The berberine and gallotannine mixtures are superior to the berberine or gallotannin treatments alone, and are more effective in controlling plant bacterial disease. In particular, the berberine and gallotannin mixtures are useful for controlling bacterial disease, especially tomato berries, tomato blight, cabbage blight, Kiwi ulcer disease, citrus ulcer disease, peach bacteriostatic germs and potato scabs.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for controlling a broad-spectrum plant bacterium that contains a natural product as an active ingredient and a use thereof.

The present invention relates to a composition for controlling a wide range of plant bacterium diseases containing natural products as an active ingredient and a use thereof and, more particularly, to a composition for controlling plant bacterium which contains a mixture of berberine and gallotannin as an active ingredient .

As antibiotics are used mainly for preventing bacterial diseases worldwide, antibiotics are mainly used. In 2011, agricultural antibiotics consuming 25,369 million won increased to 34,766 million won in 2012. The overuse of antibiotics not only causes resistance of hospital bacteria but also causes super bacteria to appear due to overuse of stronger antibiotics due to tolerance, so it is urgent to develop a safe alternative material to replace mycine antibiotics.

In order to prevent the emergence of resistant bacteria due to the indiscriminate use of antibiotics for the control of plant bacterial diseases and to develop eco-friendly farm materials that can reduce the toxicity and environmental pollution, it is urgently needed to develop antibiotics for plant natural materials The research on new technology replacing the microbial antibiotics that can cause dangerous bacteria such as super bacteria caused by the emergence of resistant bacillus has developed natural products such as berberine and gallotannine and is safe both in terms of human and environmental toxicity It is a known substance.

On the other hand, bacterial pathogens such as Claviabacter microorganism Clavibacter michiganensis subsp. michiganensis ) is a pathogenic bacterium causing Bactril canker in pepper and tomato. Tomato ulcer disease is caused by the lower leaf sagging at the beginning of development, necrosis on the edge of the young leaf, progressing gradually, twisting the leaf and dying. The stalk forms brown streaks, ulcer symptoms, and white blister spots on the leaves. Dark brown protruding rims are formed around the spots due to necrotic tissue. The fruit has a white border with brown spots, forming a fringe pattern.

Ralstonia solanacearum is a botanical bacterium that causes blight (foot blight) in over 450 plants, including major crops, in areas such as tropical and temperate climates [Hayward, AC, Biology and epidemiology of bacteria wilt caused by Pseudomonas solanacearum (1991)]. [Hayward, AC, The host of Pseudomonas solanacearum (1994)], a subspecies that can adapt to low temperatures with potato as a host in Europe in recent years, has been the most damaging to branches and crops, especially in hot and humid environments [Olsson, K.] Experience of brown rot caused by Pseudomonas solanacearum (Smith) in Sweden, Janse, JD et al. 1998. Experiences with bacterial brown rot Ralstonia solanacearum biovar 2, race 3, in The Natherland, Van Elsas, JD 2000. Survival of Ralstonia solanacearum biovar 2, the causative agent of potato brown rot, in field and microcosm soils in temperate climates (1976). Since the first potato foot blight was discovered in Jeju in July 1998, potato foot blight has occurred all over the southern part of Korea. In the case of pepper and tomato, foot blight has occurred from a long time ago, and the frequency of occurrence is very high recently.

Xanthomonas Hospital of Campus Hospital campestris pv. Black bubyeong by campestris) is to penetrate the radish, cabbage, cauliflower, turnips, bok choy and many leaves, stems, and flower stalk and roots of cruciferous crops, causing a black bubyeong. Among these, cabbage, broccoli and carlyflower have the greatest damage. These pathogens survive in the soil where the diseased plant grew and then penetrate new plants or spread through the seeds of diseased plants. In the leaves, intrusion is made through the hand, and the root penetrates through the wound.

Xanthomonas Hospital, Mantas Campus Hospital campestris pv. vesicatoria ) appears as a small grayish brown spot on the leaf at first, and the center turns white when it progresses, and the edge of the lesion becomes dark brown. A yellow border is formed around the lesion. In severe cases, the entire leaf turns brown. Brownish round spots are formed in the petiole and the petiole, and when it progresses, it enlarges slightly to dark brown irregular shape. In the fruit of bell pepper, brown to dark brown spots appear.

≪ RTI ID = 0.0 > Pectobacterium < / RTI > carotovorum subsp. carotovorum ) occurs on the base of the leaves at the beginning of the acute spots, and when it develops, it turns into a pale brown to grayish brown amorphous lesion, which begins to decay and gradually grows to the upper side of the leaf. The infected area rotates and stinks. A badly diseased tree decays entirely.

Pseudomonas ache hospitals type liquid Santini diae (Pseudomonas syringae pv. Actinidiae caused by kiwi ulcers lasted from winter to spring, and from February to early February, the shoots of the shoots stretched last year seemed to suck the water, and a reddish-brown ulcer-like scar was formed, which turned brown to black It dies dry.

Xanthomonas Hospital Type of Hospital campestris pv. The citrus peanut disease caused by Citri is gradually becoming larger and shiny on the surface due to the small spots on the back side of the yellowish oil stain. When the disease progresses, the lesion (swollen lesion) swells and the epidermis breaks. The old lesion is discolored to grayish white in the center, and a pattern is formed around it. The pathogen (Xanthomonas citri) is a short bacterium (rod bacterium) with one unipolar hair and a cyst, which in winter grows on diseased parts of the leaves, branches and fruit of the plant. The invasion of pathogens is through plant pores and wounds.

Xanthomonas, a hospital type of Jantou Monas Campestris campestris pv. Pruni ) peach bacterial pericarditis occurs on the leaves with pale green, red, and small lesions, gradually enlarged to brown discolor and dries. There is a layer between the brown lesion and the dry area, and eventually the lesion falls off and the hole opens. The branches have dark brown spots and acuminate spots. The diseased areas gradually become depressed and become ulcerated. The fruit becomes small brown spots gradually becoming dark brownish, slightly hollow indefinite lesion.

Potato scab disease caused by Streptomyces scavies occurs only on the trunk and occurs by parasitism of actinomycetes (actinomycetes). Citrus, tea, and many other plants are caused by parasitism of the fungus of the Sphaceloma genus. Citrus fruits and potatoes are particularly harmful and deteriorate in appearance, resulting in poor product value.

As an example of a technique related to the control effect of botanical pathogens of berberine or gallotannin, Korean Patent Publication No. 2006-0106958 discloses a composition for controlling a plant pathogenic bacterium containing phytophthora, Huangbiao, sanguinaria, And Korean Unexamined Patent Publication No. 2002-0000272 discloses a novel bervarubin derivative having antibacterial and antifungal activity and a salt thereof, Japanese Patent Application Laid-open No. 2013-124223 discloses a berverin derivative and a salt thereof, And derivatives thereof, and an antimicrobial agent containing magnesium oxide. However, the present invention relates to a composition for controlling a broad-spectrum plant bacterium which contains the natural product of the present invention as an active ingredient and a technology for its use Are not mentioned.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and an object of the present invention is to provide a composition for controlling a plant bacterium which contains berberine and gallotannin as an active ingredient, wherein the berberine and gallotannin mixture The present inventors have completed the present invention by confirming that the plant disease control effect is superior to the treatment with berberine or gallotannin alone.

In order to achieve the above object, the present invention provides a composition for controlling a plant bacterium which contains a mixture of berberine and gallotannin as an active ingredient.

In addition, the present invention provides a method for controlling a plant bacterial disease by treating the plant or plant soil with a composition for controlling plant disease.

The present invention relates to a composition for controlling a wide range of plant bacterium diseases containing natural products as an active ingredient and a use thereof and, more particularly, to a composition for controlling plant bacterium which contains a mixture of berberine and gallotannin as an active ingredient .

The berberine and gallotannin mixtures according to the present invention are more effective than the berberine and gallotannin alone in improving the control of plant bacteriological diseases and have an effect on a wide range of plant bacterial diseases, It can be used as an environmentally friendly antibacterial agent which can prevent the appearance of resistant bacteria, reduce the lethal toxicity and the environmental pollution.

FIG. 1 shows the results of confirming the effect of streptomycin on the control of plant diseases as a mixture of berberine and gallotannin according to the present invention, berberine, gallotannin, and a positive control.

The present invention relates to a composition for controlling a plant bacterium which contains a mixture of berberine and gallotannin as an active ingredient.

The plant bacterium includes plant diseases caused by a wide range of phytopathogenic bacteria, and is a plant disease caused by Gram-negative bacteria and Gram-positive bacteria. In particular, the berberine and gallotannin mixture, which are effective ingredients of the present invention, But is not limited to, at least one plant disease selected from the group consisting of bacterial diseases such as brucellosis, bacterial blight, bacterial floret disease, kiwifruit disease, citrus ulcer disease, peach bacterial vascular disease and potato scab disease.

Wherein the berberine is any thing which extracts from natural products other than the preferably separated from the extract extracted from the roots and stems of longitude linden the hwangbaekpi or goldthread (Coptis chinensis) shell of (Phellodendron amurense), but not limited to, chemical synthesis or hwangbaekpi much Likewise, galantanin is also known as gall bladder ( Galla rhois or Galla chinensis ) extract, but the present invention is not limited thereto, and it can be chemically synthesized or separated from natural products other than gala rois.

In the liquid formulation, the mixing ratio of berberine and gallotannin is preferably 1: 25 to 50, more preferably 1: 30, and the mixing ratio is 1: 25 to 50: , There is a synergy effect on the plant disease control effect.

The berberine and gallotannine are preferably dissolved in water, a C ₁ to C ₄ lower alcohol or a mixture thereof. However, the present invention is not limited thereto. When a mixture of water and a C ₁ to C ₄ lower alcohol is used , And the mixing ratio is 1: 0.5 to 2 by volume. However, the mixing ratio is not limited thereto. More preferably, a mixture of water and ethanol is mixed at a volume ratio of 1: 0.5 to 2.

The composition may contain 10 to 400,000 ppm of a mixture of berberine and gallotannin as an active ingredient, but the present invention is not limited thereto. The composition may be used by concentrating or diluting it as necessary. However, when the dilution is less than 10 ppm, If it exceeds 400,000 ppm, there may be a problem that an unnecessary process of concentration at a higher concentration than necessary is carried out. Preferably, the composition may contain from 5,000 to 400,000 ppm of a mixture of berberine and gallotannine as an active ingredient.

In addition to the berberine and gallotannin mixtures, the composition for controlling plant pathogenic bacterium of the present invention may also contain a pesticidally acceptable solid carrier, a liquid carrier, a liquid diluent, a liquefied gas diluent, a solid diluent or other suitable adjuvant, A surfactant such as an emulsifier, a dispersant, or a foaming agent may be further included. A composition for controlling botanical bacterium in which a mixture of berberine and gallotannin and the above excipient are mixed can be formulated into various formulations known in the field of agrochemicals and can be used in any formulations conventionally used in the field of agrochemicals .

The composition for controlling botanical bacterium of the present invention may be formulated into a form of a wettable powder, a granule, a powder, an oil, a spray, a film, a capsule or a gel, but is not limited thereto.

In addition, the present invention provides a method for controlling a plant bacterial disease by treating the plant or plant soil with a composition for controlling plant disease. The botanical bacterium can be, but is not limited to, one or more botanical bottles selected from tomato bug disease, tomato bug blight, cabbage bruise, chilli bacterial blight, cabbage blight, kiwi bruise, citrus brucellosis, .

Plant Bacterial Disease and Scientific Name Plant bacterium Scientific name Gram stain reaction Tomato
(Bacterial canker) Clavibacter michiganensis subsp. michiganensis + Tomato foot rot disease
(Bacterial wilt) Ralstonia solanacearum - Cabbage Blackbug
(Black rot) Xanthomonas campestris pv. campestris - Red pepper bacterial spotty disease
(Bacterial Spot) Xanthomonas campestris pv. vesicatoria - Cabbage rolls
(Bacterial soft rot) Pectobacterium carotovorum subsp. carotovorum - Kiwi
(Bacterial canker) Pseudomonas syringae pv. actinidiae - Citrus peel
(Bacterial canker) Xanthomonas campestris pv. citri - Peach hole
(Bacterial canker) Xanthomonas campestris pv. pruni - Potato scab
(Common scab) Streptomyces  scabies +

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Example  One. Berber , Gallotannine  And tomato juvenile germs using the mixture Clavibacter michiganensis subsp . michiganensis ) Of control effect

Tomato Ulcer Bacteria ( Clavibacter michiganensis subsp. michiganensis ) was cultured in R2B medium (0.5 g yeast extract, 0.5 g protease peptone No. 3, 0.5 g Casamino acids, 0.5 g Glucose, 0.5 g Soluble starch, 0.3 g of sodium pyruvate, 0.3 g of K ₂ HPO ₄ , 0.05 g of MgSO ₄ .7H ₂ O and 1 L of distilled water were used. After filtration using a 0.2 μm syringe filter, And streptomycin as a control were added to the R2B medium to prepare a medium solution medium. Thereafter, the tomato bacillus strain ( Clavibacter michiganensis subsp. michiganensis ) was inoculated into each of the above chemical solution media, and cultured for 24 hours and 48 hours in a shaking incubator (30 DEG C, 150 rpm). OD ₆₀₀ values were measured by a spectrophotometer (Table 2). The OD ₆₀₀ of each of the most effective 30% gallotannine and 1% berberine drug was measured, and the mixed solution thereof was treated so as to have a final concentration of 50 ppm, 100 ppm, and 200 ppm, whereby the untreated and positive control Strepto And compared with mycotoxin.

As a result, as shown in Fig. 1, Table 3 and Table 4, the berberine and gallotannin mixture, which are the active ingredients of the present invention, are more effective than berberine or gallotannin alone, michiganensis subsp. michiganensis ), and compared with the streptomycin treatment, which is a positive control, almost similar tomato gum disease ( Clavibacter michiganensis subsp. michiganensis ).

Determination of Antibacterial Activity against Tomato Ulcer Bacteria ( Clavibacter michiganensis subsp. Michiganensis ) by Mixing Ratio of Berberine and Galotannin Mixing ratio (% by weight) OD ₆₀₀ measured value Gallotannine Berber 24hr 48hr 30% 0.5% 0.023 0.032 30% 1.0% 0.004 0.016 30% 1.5% 0.009 0.021 25% 0.5% 0.031 0.042 25% 1.0% 0.022 0.026 25% 1.5% 0.018 0.031 25% 2.0% 0.012 0.023 20% 0.5% 0.046 0.057 20% 1.0% 0.038 0.042 20% 1.5% 0.036 0.052 20% 2.0% 0.018 0.027 The control (streptomycin) 0.011 0.031 No treatment 1.094 1.578

Primary antibacterial activity (measured by OD ₆₀₀ ) according to berberine and gallotannin concentration (technical)
Berberine
1% aqueous solution Galotannine
30% aqueous solution Control
(Streptomycin) No treatment 50 ppm 100ppm 200ppm 50 ppm 100ppm 200ppm 50 ppm 100ppm 200ppm No treatment Tomato 0.784 0.638 0.484 0.507 0.321 0.270 0.041 0.023 0.012 1.253 Tomato foot rot disease 0.822 0.801 0.754 0.654 0.573 0.421 0.038 0.013 0.008 1.352 Cabbage Blackbug 0.521 0.487 0.428 0.514 0.487 0.399 0.024 0.019 0.011 1.082 Red pepper bacterial spotty disease 0.635 0.541 0.455 0.681 0.612 0.555 0.053 0.024 0.019 1.311 Cabbage rolls 0.953 0.878 0.724 0.537 0.472 0.313 0.038 0.012 0.0007 1.524

The berberine tannate antibacterial activity (OD ₆₀₀ measured value) produced by the ester bond after the mixed preparation of berberine and gallotannine
1% berberine + 30% gallothannine
(Berber tannate) Control
(Streptomycin) No treatment 50 ppm 100ppm 200ppm 50 ppm 100ppm 200ppm No treatment Tomato ulcer disease 0.061 0.041 0.032 0.041 0.021 0.009 1.529 Tomato foot rot fungus 0.082 0.072 0.052 0.028 0.019 0.011 1.411 Cabbage blackhead germ 0.074 0.031 0.028 0.033 0.028 0.013 1.154 Bacillus 0.065 0.052 0.033 0.022 0.013 0.008 1.325 Cabbage rot fungus 0.042 0.036 0.012 0.048 0.029 0.014 1.531 Kiwi ulcer disease 0.098 0.073 0.051 0.038 0.016 0.007 1.448 Citrus ulcer disease 0.054 0.048 0.024 0.023 0.014 0.008 1.258 Peach bacterial hole bacteria 0.063 0.055 0.039 0.036 0.025 0.016 1.356 Potato scab 0.108 0.085 0.062 0.047 0.031 0.004 1.432

Example  2. Berber , Gallotannine  And a mixture thereof. Ralstonia  solanacearum ) Of control effect

The culture medium of Ralstonia solanacearum was prepared by adding 1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl, 0.45 g of KH ₂ PO ₄ , Na ₂ HPO ₄ · 12H ₂ O, and 1 L of distilled water), filtered through a 0.2 쨉 m syringe filter, and a mixture of berberine and gallotannin mixed at various weight ratios and streptomycin as a control were added to the NBP medium To prepare a chemical liquid medium. Since then, two days put tomato blight bacteria cultured in medium NBP (Ralstonia solanacearum ) was inoculated into each of the above chemical solution media, and cultured in a shaking incubator (30 ° C., 150 rpm) for 24 hours and 48 hours. OD ₆₀₀ values were measured by a spectrophotometer (Table 5). The OD ₆₀₀ of each of the most effective 30% gallotannine and 1% berberine drug was measured, and the mixed solution thereof was treated so as to have a final concentration of 50 ppm, 100 ppm, and 200 ppm, whereby the untreated and positive control Strepto And compared with mycotoxin.

As a result, as shown in Fig. 1, Table 3 and Table 4, the active ingredient of the present invention, berberine and gallotannin, showed a better control effect against Ralstonia solanacearum than berberine or gallotannin alone ( Ralstonia solanacearum ) against streptomycin, which is a positive control, and it has been confirmed that it is effective to control Ralstonia solanacearum .

Determination of Antibacterial Activity against Ralstonia solanacearum by the Mixing Ratio of Berberine and Galotannin Mixing ratio (% by weight) OD ₆₀₀ measured value Gallotannine Berber 24hr 48hr 30% 0.5% 0.031 0.042 30% 1.0% 0.016 0.019 30% 1.5% 0.013 0.021 25% 0.5% 0.058 0.072 25% 1.0% 0.042 0.058 25% 1.5% 0.039 0.051 25% 2.0% 0.023 0.023 20% 0.5% 0.062 0.077 20% 1.0% 0.058 0.061 20% 1.5% 0.032 0.064 20% 2.0% 0.021 0.028 The control (streptomycin) 0.002 0.008 No treatment 1.124 1.764

Example  3. Berber , Gallotannine  And a mixture thereof. Pectobacterium carotovorum subsp . carotovorum ) Of control effect

Pectobacterium carotovorum subsp. carotovorum were cultured in NB medium (1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl and 1 l of distilled water), filtered using a 0.2 μm syringe filter, Streptomycin as a control and a mixture of berberine and gallotannin mixed at various weight ratios were added to the above NB medium to prepare a chemical liquid medium. After incubation for 2 days in NB medium, Pectobacterium carotovorum subsp. carotovorum ) was inoculated into each of the above drug solutions, and cultured in a shaking incubator (30 ° C, 150 rpm) for 24 hours and 48 hours. OD ₆₀₀ values were measured by a spectrophotometer (Table 6). The OD ₆₀₀ of each of the most effective 30% gallotannine and 1% berberine drug was measured, and the mixed solution thereof was treated so as to have a final concentration of 50 ppm, 100 ppm, and 200 ppm, whereby the untreated and positive control Strepto And compared with mycotoxin.

As a result, as shown in Fig. 1, Table 3 and Table 4, the berberine and gallotannin mixture, which are the active ingredients of the present invention, was more effective than berberine or gallotannin alone in treating Pectobacterium carotovorum subsp. carotovorum ), and it was confirmed that Pectobacterium carotovorum subsp. carotovorum , which is almost similar to streptomycin treatment, is effective for control.

Antibacterial activity of Pectobacterium carotovorum subsp. Carotovorum according to the ratio of berberine and gallotannin Mixing ratio (% by weight) OD ₆₀₀ measured value Gallotannine Berber 24hr 48hr 30% 0.5% 0.043 0.082 30% 1.0% 0.036 0.058 30% 1.5% 0.033 0.061 25% 0.5% 0.060 0.069 25% 1.0% 0.051 0.063 25% 1.5% 0.046 0.061 25% 2.0% 0.038 0.059 20% 0.5% 0.058 0.065 20% 1.0% 0.048 0.053 20% 1.5% 0.051 0.059 20% 2.0% 0.046 0.058 The control (streptomycin) 0.012 0.017 No treatment 0.896 1.276

Example  4. Berber , Gallotannine  And mixtures thereof. Xanthomonas campestris pv . campestris ) Of control effect

Cabbage Blackbird ( Xanthomonas campestris pv. campestris ) was prepared by using NB medium (1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl and 1 L of distilled water) (Final concentrations of 50, 100 and 200 ppm), berberine (final concentrations of 50, 100 and 200 ppm), galantannin (final concentrations of 50, 100 and 200 ppm), and a mixture of berberine and galantannin 200 ppm) and streptomycin (final concentration: 50, 100, 200 ppm) were added as a control. After this, the cabbage black broth ( Xanthomonas campestris pv. campestris) by taking the 100㎕, and inoculated into culture medium of the drug solution, each, and then cultured for 24 hours in a shaking incubator (30 ℃, 150rpm), the OD ₆₀₀ value with a spectrophotometer (spectrophotometer) were measured.

As a result, control of the berberine and Gallo tannin mixture cabbage heukbu bacteria (Xanthomonas campestris pv. Campestris), compared to treatment with berberine or Gallo tannin sole active ingredient of the present invention as disclosed in Figure 1, Table 3 and Table 4 was confirmed to have excellent effects, it was confirmed that represents the control efficacy of a positive control of streptomycin (streptomycin), and treated almost similar cabbage heukbu germs compared (Xanthomonas campestris pv. campestris).

Example  5. Berber , Gallotannine  And a mixture thereof were used for the bacterial fungus Xanthomonas campestris pv . vesicatoria ) Of control effect

Red pepper bacterial fungus ( Xanthomonas campestris pv. vesicatoria were cultured in NB medium (1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl and 1 l of distilled water) (Final concentrations of 50, 100 and 200 ppm), galantanin (final concentration of final concentration 50, 100, 200 ppm) and berberine (galactomannan, 100, 200 ppm) and streptomycin (final concentration 50, 100, 200 ppm) as a positive control were added to prepare a drug solution medium. Thereafter, the bacteria were cultured for 2 days in a hot pepper bacterium, Xanthomonas campestris pv. vesicatoria) taking the 100㎕, and inoculated into culture medium of the drug solution, each, and then cultured for 24 hours in a shaking incubator (30 ℃, 150rpm), the OD ₆₀₀ value with a spectrophotometer (spectrophotometer) were measured.

As a result, FIG. 1, Table 3 and Table 4, the active ingredient, the tannin berberine and Gallo mixture of the invention, as will pepper bacterial jeommunuibyeong compared to treatment with tannin berberine or Gallo alone bacteria described in (Xanthomonas campestris pv. vesicatoria ), and compared with the streptomycin treatment, which is a positive control, Xanthomonas spp. campestris pv. vesicatoria ).

Example  6. Berber , Gallotannine  And a mixture thereof Pseudomonas syringae pv . actinidiae ) Of control effect

Kidney ulcers ( Pseudomonas syringae pv. actinidiae The culture medium consisted of NBP medium (1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl, 0.45 g of KH ₂ PO ₄ , 2.39 g of Na ₂ HPO ₄ .12H ₂ O, A mixture of berberine and galantannin (final concentrations of 50, 100 and 200 ppm) mixed at a weight ratio of 1:30 of the test agent filtered through a 0.2 쨉 m syringe filter was used in the NBP medium and streptomycin Final concentrations of 50, 100 and 200 ppm) were added to the NBP medium to prepare a medium culture medium. After that, the cells were cultured for 2 days in the presence of Pseudomonas syringae pv. actinidiae) taking the 100㎕, and inoculated into culture medium of the drug solution, each, and then cultured for 24 hours in a shaking incubator (30 ℃, 150rpm), the OD ₆₀₀ value with a spectrophotometer (spectrophotometer) were measured.

As a result, as shown in Fig. 1 and Table 4, berberine and gallotannin mixture of the active ingredient of the present invention, Pseudomonas syringae pv. actinidiae ), and compared with the streptomycin treatment, which is a positive control, almost the same effect was observed for Pseudomonas spp. syringae pv. actinidiae ), respectively.

Example  7. Berber , Gallotannine  And citrus ulcer disease using mixtures thereof Xanthomonas campestris pv . citri ) Of control effect

Citrus ulcer disease ( Xanthomonas campestris pv. citri were used for NB medium (1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl and 1 l of distilled water) Streptomycin (final concentration 50, 100, 200 ppm) was added to the NB medium as a positive control, and a mixture of berberine and galantannin (final concentration 50, 100, 200 ppm) mixed at a weight ratio of 1:30 of the filtered test agent To prepare a chemical liquid medium. Thereafter, the citrus ulcerative germs ( Xanthomonas campestris pv. citri) taking the 100㎕, and inoculated into culture medium of the drug solution, each, and then cultured for 24 hours in a shaking incubator (30 ℃, 150rpm), the OD ₆₀₀ value with a spectrophotometer (spectrophotometer) were measured.

As a result, as shown in Fig. 1 and Table 4, the active ingredient of the present invention, berberine and a mixture of galantanin , citrus ulcer germ ( Xanthomonas campestris pv. citri ), and compared with the streptomycin treatment, which is a positive control, citrus ulcer disease ( Xanthomonas campestris pv. citri ), respectively.

Example  8. Berber , Gallotannine  And a mixture thereof, Xanthomonas campestris pv.  pruni )of  Check control effect

Peach Bacterial Hole Gonorrhea ( Xanthomonas campestris pv. pruni ) was used as the culture medium of NB medium (1 g of "Lab-Lemco" beef extract, 2 g of yeast extract, 5 g of peptone, 5 g of NaCl and 1 l of distilled water) Streptomycin (final concentrations of 50, 100 and 200 ppm) as a positive control was added to the NB medium (final concentrations: 50, 100 and 200 ppm) and a mixture of berberine and galantannin mixed at a weight ratio of 1:35 of the filtered test agent To prepare a chemical liquid medium. Thereafter, the cultured peach bacterium ( Xanthomonas campestris pv. pruni) taking the 100㎕, and inoculated into culture medium of the drug solution, each, and then cultured for 24 hours in a shaking incubator (30 ℃, 150rpm), the OD ₆₀₀ value with a spectrophotometer (spectrophotometer) were measured.

As a result, as shown in Fig. 1 and Table 4, it was confirmed that the berberine and gallotannin mixture of the active ingredient of the present invention, peach bacteriostatic bacteria ( Xanthomonas campestris pv. pruni ), and compared with the streptomycin treatment, which is a positive control, almost similar peat bacterial periosteum ( Xanthomonas campestris pv. pruni ) was observed.

Example  9. Berber , Gallotannine  And mixtures thereof. Streptomyces  scabies ) Of control effect

The culture medium of Streptomyces scabies was a GYM medium (4 g of glucose, 4 g of yeast extract, 10 g of malt extract, 2 g of CaCO 3 and 1 l of distilled water). The GYM medium was filtered using a 0.2 μm syringe filter Streptomycin (final concentration 50, 100, 200 ppm) as a positive control was added to the GYM medium (final concentration 50, 100, 200 ppm) and a mixture of berberine and gallotannin mixed at a weight ratio of 1:30 Medium was prepared. Thereafter, the cells were incubated for 2 days with Streptomyces scabies ) were inoculated into each of the above-mentioned drug solutions, cultured in a shaking incubator (30 ° C, 150 rpm) for 24 hours, and then the OD ₆₀₀ value was measured by a spectrophotometer.

As a result, as shown in FIG. 1 and Table 4, it was confirmed that the effect of controlling the Streptomyces scabies of berberine and gallotannin mixture, which is an effective ingredient of the present invention, was excellent, and streptomycin treatment The results showed that the control effect of Streptomyces scabies was almost similar to that of Streptomyces scabies .

Claims (7)

At least one plant bacterium selected from the group consisting of a mixture of berberine and gallotannin mixed at a weight ratio of 1: 20 to 40, as an active ingredient, selected from the group consisting of tomato, bark wilt, cabbage bruise, red pepper brucellosis, (2). delete delete The method of claim 1, wherein the berberine and gallotannine are dissolved in water, a C ₁ to C ₄ lower alcohol, or a mixture thereof, wherein the berberine and gallotannine are dissolved in water, a C ₁ to C ₄ lower alcohol or a mixture thereof. At least one plant bacterium. The composition according to claim 1, wherein the composition comprises 5,000 to 400,000 ppm of a mixture of berberine and gallotannin as an active ingredient, wherein the composition contains at least one selected from the group consisting of tomato brucellosis, tomato blight, cabbage bruise, A composition for controlling plant bacterium diseases. A plant bacterium disease prevention composition according to any one of claims 1, 4, and 5 is applied to a soil planted with plants or plants to produce a plant for preventing or treating diseases such as tomato bugs, tomato bugs, cabbage bugs, A method for controlling one or more selected plant bacterial diseases. delete

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