KR101873292B1 - Composition for reducing hangover containing gynostemma pentaphyllum leaf extract - Google Patents

Abstract

The present invention relates to a hangover resolution composition containing a stone-to-leaf extract, and more particularly, to a hangover composition containing an extract of a stone-on-the-fly, and more particularly to a composition for improving hangover, The present invention relates to a hangover resolution composition containing an extract of a stone-to-the-leaf.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a hangover-

The present invention relates to a hangover resolution composition containing a stone-to-leaf extract, and more particularly, to a hangover composition containing an extract of a stone-on-the-fly, and more particularly to a composition for improving hangover, The present invention relates to a hangover resolution composition containing an extract of a stone-to-the-leaf.

Alcohol absorbed into the body through alcohol is converted to acetaldehyde by alcohol dehydrogenase and then decomposed into acetic acid by aldehyde dehydrogenase and finally decomposed into water and carbon dioxide. In this process, if the alcohol is lacking, acetaldehyde, a carcinogen, remains in the body and causes hangover. When a hangover occurs, a hangover resolution composition capable of promoting alcohol decomposition or removing substances caused by alcohol metabolism has been developed as in the following patent documents, accompanied by a significant discomfort such as headache, heartburn, and motion sickness.

<Patent Literature>

Patent Publication No. 10-1034986 (registered on May 09, 2011) "Composition for hangover decontamination containing mixed herbal extract as an active ingredient"

However, in the conventional hangover resolution composition, the hangover resolution effect is inferior, the extraction efficiency of the active ingredient is low, and many materials are required, and the bacteria are easily proliferated, shortening the storage period.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems,

It is an object of the present invention to provide a hangover resolution composition containing an extract of a stone on the leaf containing an extract of hydrolyzed water and a stone-to-leaf extract and having an improved hangover resolution effect.

It is also an object of the present invention to provide a hangover resolution composition containing an extract of a stone-on-the-leaf having an improved antioxidant and anti-inflammatory effect.

It is another object of the present invention to provide a hangover solution containing hydrogen peroxide, which can inhibit bacterial growth and increase the storage period.

The present invention also relates to a method for extracting water-soluble useful components from alcohols by first extracting the oil-soluble useful components by alcohol extraction, The present invention provides a hangover rescue composition containing an extract of a stone-on-the-fly.

In order to achieve the above object, the present invention is implemented by the following embodiments.

According to one embodiment of the present invention, the hangover resolution composition containing the extract from the stone of the present invention according to the present invention is characterized in that it contains hydrogen peroxide and an extract from the stone.

According to another embodiment of the present invention, in the hangover resolution composition containing the extract from the outer part of the stones of the present invention, the hydrogenated water is formed by injecting and dissolving hydrogen gas into water.

According to still another embodiment of the present invention, in the hangover resolution composition containing the extract from the outer part of the stalk of the present invention, the hydrogen concentration is characterized by a hydrogen gas concentration of 100 to 1500 ppb.

According to still another embodiment of the present invention, in the hangover resolution composition containing the extract of the outer layer of stones according to the present invention, the extract of the outer layer of stones is characterized in that hydrogen is used as an extraction solvent.

According to another embodiment of the present invention, in the hangover resolution composition containing the extract of the outer layer of stones according to the present invention, the outer layer of the extract of the stones may be obtained by forming an extract of the outer layer of stones from the outer leaves using alcohol as an extraction solvent, And water of a predetermined temperature is used as an extracting solvent to form a small amount of extraneous leaf water, and the extragranular water of the leaf water and the extract of the extruded alcohols are mixed with each other.

According to still another embodiment of the present invention, the hangover rescue composition containing the extract from the outer layer of stones according to the present invention is characterized by having antioxidant and anti-inflammatory effects.

According to the present invention, the following effects can be obtained by this embodiment.

The present invention has an improved hangover resolution effect by containing hydrolyzed water and a stone-leaf extract.

In addition, the present invention has an improved antioxidant and anti-inflammatory effect.

Further, the present invention has the effect of inhibiting the growth of bacteria and increasing the storage period by including hydrogen water.

The present invention also relates to a method for extracting water-soluble useful components from alcohols by first extracting the oil-soluble useful components by alcohol extraction, There is an effect.

Hereinafter, the hangover resolution composition containing the extract from the stone of the present invention according to the present invention will be described in detail with reference to the accompanying drawings. Unless defined otherwise, all terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs and, if conflict with the meaning of the terms used herein, It follows the definition used in the specification. Further, the detailed description of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted. Throughout the specification, when an element is referred to as "including " an element, it is understood that the element may include other elements as well, without departing from the other elements unless specifically stated otherwise.

The hangover resolution composition according to one embodiment of the present invention comprises an extract of the outer layer of stone and hydrogenated water. The hangover resolution composition may be prepared by any of the formulations conventionally produced in the art. For example, the hangover resolution composition may be prepared in a beverage form.

Gynostemma pentaphyllum is a perennial vine plant with dicotyledonous plant leaves. Leaves grow in mountains or forests. Roots grow sideways, with white hairs on the nodes, tangled and growing, leaves are staggered, It is a compound leaf with leaves and it has a narrow egg-shaped oval or narrow oval shape. The hydrogenated water refers to water containing hydrogen gas in water. Hydrogen may be contained in water through electrolysis. However, in order to minimize the content of impurities, hydrogen is directly injected into water and dissolved. It is preferable that the hydrogen concentration is such that the dissolved concentration of the hydrogen gas (H ₂ ) is at least 100 ppb to at most 1500 ppb.

Preferably, the above-mentioned out-of-stone-leaf extract is formed of an extraneous water-in-water or an out-of-stone alcohol extract, and an alcohol such as ethanol is used as an extraction solvent to form an out- (70 to 90 ° C) is used as an extraction solvent to form a water extract of extraneous leaf water, and it is more preferable that the extract is formed by mixing the water extract of the extreme off-the-leaf water and the extract of the extracellular alcohols.

The extracellular step extract is subjected to alcohol extraction and then subjected to extraction with hydrogen peroxide at a temperature of 70 to 90 ° C. This is because if heat is first applied to the extraction of hydrogen peroxide, And water extraction is performed first, there arises a problem that the percentage of water contained in the stone outer leaves varies. To prevent this, it is preferable to perform alcohol extraction and then water extraction.

The above-mentioned extracellular alcohols extract and extragranular alfalfa extracts can be prepared as follows. The outer side of the stone is cleaned with water, dried in the shade or sunlight, and pulverized to an appropriate size, and the crushed stone outer leaf is treated with a polar solvent selected from a 10-fold weight of a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, Ethanol is preferably used, more preferably 70% alcohol is used for leaching for 10 to 30 days, and then filtered to produce an extract of alveoli. It is understood that such an extract of alcohol such as lipids, which are not soluble, is highly extracted. In addition, after obtaining a stone-leaf alcohol extract, hydrolyzed water is added to the remaining amount of the stones, the mixture is stirred at 70 to 90 ° C for 2 to 6 hours, and then filtered to produce an extraneous leaf water extract. The extraction of water - soluble polysaccharides and other substances are highly appreciated by the extraction of water, and it is understood that the extraction efficiency of the useful components is increased when using the hydrophobic water.

According to the present invention, the hangover resolution composition contains hydrolyzed water and extract from the stomach leaf to improve the hangover effect. In addition, it has antioxidant, anti-inflammatory effect, inhibits the proliferation of bacteria by hydrogen peroxide, And extracting the useful components by extracting both the fat soluble component and the water soluble useful component by extracting the alcohol with water followed by extracting the useful component by using water instead of purified water. These effects will be described in detail in the following examples.

The hangover resolution composition of the present invention may further comprise a fruit concentrate useful for eliminating hangovers, and a hangover solubility fraction such as honey.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these are only for the purpose of illustrating the present invention in more detail, but the scope of the present invention is not limited thereto.

&Lt; Example 1 >

The 20 days immersion of the crushed stone oeip a 70% ethanol of 10 times the weight after the filtering by the dissolved stone oeip alcohols preparing the extract, filtered, and the number of 10 times the weight of the remaining stones oeip remaining minority (hydrogen gas (H ₂₎ And the mixture was heated at 80 DEG C for 4 hours with stirring. The resulting mixture was filtered to prepare an extraneous leaf water extract, which was then mixed with the extract of the extreme off-the-leaf water and the extract of the extruded alcohols at a ratio of 1: 1, Concentrated and lyophilized to produce an extract from the stones.

&Lt; Example 2 >

The crushed stone outer leaves were immersed in 70% ethanol of 10 times weight for 20 days and then filtered to prepare an extract of the stone outer alveoli. To the remaining amount of the remaining stone outer leaves, purified water having a weight of 10 times the weight was added and stirred at 80 ° C for 4 hours After the addition of heat, the extract was filtered to prepare a purified water extract of stony leaves. The purified water extract of stony leaves and the extract of stony leaves were mixed in a ratio of 1: 1, concentrated under reduced pressure, and lyophilized to produce an extract of stony leaves.

&Lt; Comparative Example 1 &

The crushed stone outer leaves were dipped in 10 times by weight of 70% ethanol for 20 days, and then filtered to produce an extract of the stalk extrudate. The concentrate was concentrated under reduced pressure and lyophilized to give an extract of the stalk.

&Lt; Comparative Example 2 &

The crushed stone outer leaves were placed in a 10-fold weight of hydrogen peroxide (the dissolved concentration of hydrogen gas (H ₂ ) was 1500 ppb), heated at 80 ° C for 4 hours while stirring, filtered to produce an extraneous leaf water water- And lyophilized to produce an extract from the stone.

&Lt; Comparative Example 3 &

Hydrogen water (hydrogen gas (H ₂ ) dissolved concentration of 1500 ppb) of 10 times the weight of the crushed stone outer leaf was added and heated at 80 ° C for 4 hours while stirring to prepare purified water extract of stone outer leaves, The remaining stones were immersed in 10% by weight of 70% ethanol for 20 days and then filtered to prepare an extract of the outer wall of the stone. The outer water of the stone extract and the extract of the outer wall of the stone were mixed in a ratio of 1: 1, And lyophilized to produce an extract from the stone.

&Lt; Experimental Example 1 >

(1) A hangover resolution composition (beverage form) containing the extract from the leaves of the stones prepared in Examples 1 and 2 and Comparative Examples 1 to 3 was prepared in the composition ratio shown in Table 1 below.

Content (% by weight) ingredient Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 The stone-leaf extract of Example 1 3 - - - - 3 The stone-leaf extract of Example 2 - 3 - - - - The out-of-stone extract of Comparative Example 1 - - 3 - - - The stone-leaf extract of Comparative Example 2 - - - 3 - - The stone-leaf extract of Comparative Example 3 - - - - 3 - honey 7 7 7 7 7 7 Liquid fructose 3 3 3 3 3 3 Vitamin C 0.5 0.5 0.5 0.5 0.5 0.5 Citric acid 1.0 1.0 1.0 1.0 1.0 1.0 Apple concentrate 3.0 3.0 3.0 3.0 3.0 3.0 Purified salt 0.01 0.01 0.01 0.01 0.01 0.01 Spices 0.2 0.2 0.2 0.2 0.2 0.2 Water Balance Balance Balance Balance Balance - Purified water - - - - - Balance Sum 100 100 100 100 100 100

(2) Alcohol decomposition ability evaluation using alcohol meter

1) The effect of alcohol concentration was measured by alcohol concentration test in 20 men (25-30 years old). (AL-2500, DATECH) from 1 hour after 1 hour of drinking after 3 hours of drinking with 3 mL / kg of each of Samples 1 to 5 and 22 mL of shochu at 5 mL / And the average value was calculated. The results are shown in Table 2. &lt; tb &gt; &lt; TABLE &gt;

2) Referring to Table 2, it can be seen that the samples 1 and 2 are much lower in number than the samples 3 to 5, so that the alcohol decomposition ability is high when the alcohol is extracted by using water (purified water) after extraction. In addition, it can be seen that the sample 1 is lower in value than the sample 2, and it is found that the alcohol decomposition ability is excellent when the water is used as the extraction solvent rather than the purified water.

After 1 hour
Alcohol concentration After 2 hours
Alcohol concentration After 3 hours
Alcohol concentration After 4 hours
Alcohol concentration After 5 hours
Alcohol concentration After 7 hours
Alcohol concentration control
(Alcohol only) 0.060 0.075 0.055 0.043 0.035 0.015 Sample 1 0.040 0.038 0.031 0.021 0.012 0.008 Sample 2 0.047 0.043 0.037 0.026 0.017 0.011 Sample 3 0.053 0.048 0.042 0.030 0.021 0.012 Sample 4 0.055 0.055 0.044 0.032 0.024 0.012 Sample 5 0.054 0.053 0.043 0.032 0.023 0.011

<Experimental Example 2> Evaluation of antioxidative effect by DPPH radical scavenging activity

1) Dissolve 2.5 mg of DPPH in 17 mL of ethanol to prepare a DPPH solution. To 10 mL of this solution, 5.75 mL of DMSO was added, and UV-Vis of the control group was observed at 517 nm. It was diluted with ethanol to an absorbance of 0.94-0.97 and shaken for 20 seconds. To 1 mL of the solvent, 1 mg of each of the extracts of the out of the stone produced in Examples 1 and 2 and Comparative Examples 1 to 3 was mixed and sufficiently dissolved. Then, 450 μL of the prepared DPPH was mixed with 50 μL of the sample solution and kept at room temperature for 10 minutes. Were measured. The elimination ability formula is calculated in the following manner and is shown in Table 1. Electron donating ability (%) = 100 - [(O.D. Of sample / O.D. of control)] 100

2) As shown in Table 3, it can be seen that Examples 1 and 2 are much higher in number than Comparative Examples 1 to 3, and thus the antioxidant power is high when extracted with alcohol (purified water) after alcohol extraction. In addition, it can be seen that Example 1 has a higher value than Example 2, and it can be seen that the antioxidant power is higher when water is used as the extraction solvent than purified water.

Example 1 Example 2 Comparative Example 1 Comparative Example 2 Compare to 3 Electron donating ability (%) 37 32 24 21 23

&Lt; Experimental Example 3 > Evaluation of anti-inflammatory activity by inhibiting nitric oxide formation

(1) The efficacy of the extracellular extracts of Examples 1 and 2 and Comparative Examples 1 to 3 on NO production, which is one of active oxygen and is known to play an important role in inducing inflammation, was evaluated.

(2) RAW 264.7 cells were adjusted to 2 × 10 ⁵ cells / mL using DMEM medium supplemented with 10% FBS and inoculated into a 24-well plate. LPS (1 μg / mL) were simultaneously treated and cultured for 24 hours. The amount of produced NO Griess reagent [1% (w / v) sulfanilamide, 0.1% (w / v) naphylethylenediamine in 2.5% (v / v) phosphoric acid] by using the NO ₂ present in the cell culture ^- in the form of Respectively. 100 μL of the cell culture supernatant and 100 μL of the Griess reagent were mixed and reacted on 96-well plates for 10 minutes, and the absorbance was measured at 540 nm. The amount of NO produced was compared with sodium nitrite (NaNO ₂ ) as a standard, and the results are shown in Table 4.

(3) Referring to Table 4, it can be seen that Examples 1 and 2 are much lower in value than Comparative Examples 1 to 3, and it is known that when extracted with alcohol (purified water) after alcohol extraction, have. In addition, it can be seen that Example 1 is lower in value than Example 2. It can be seen that the use of hydrogen peroxide as an extraction solvent is more effective in inhibiting the formation of NO.

control (no processing) Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 NO production% 100 75 80 86 90 90

&Lt; Experimental Example 4 > Evaluation of the effect of increasing the storage period by the experiment of measuring the number of general bacteria

(1) Samples 1 and 6 of Experimental Example 1 were allowed to stand at room temperature for 24 hours, then diluted 10-fold and 100-fold, respectively, and inoculated on sterilized standard methods agar (Difco) The number of colonies was counted by counting the colonies generated after incubation for a period of 30-300 colonies per plate. The number of colonies was counted and the number of bacteria was calculated by multiplying the number of colonies by the dilution factor. The number of bacteria in the sample 1 was 3.1 × 10 ^3, and the general bacterial count of the sample 6 was 7.5 x 10 ⁴ . Thus, it can be seen that when the hydrogenation water is used in the hangover resolution composition, the proliferation of bacteria can be suppressed and the storage period of the hangover resolution composition can be increased.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, Should be interpreted as belonging to the scope.

Claims (6)

And is formed by mixing hydrolyzed water with a stone-leaf extract,
The hydrogenated water is formed by injecting and dissolving hydrogen gas into water, the dissolved gas concentration of hydrogen gas is 100 to 1500 ppb,
The extracellular leachate extract is prepared by forming an extruded stone alcohole extract from a stone outer leaf using alcohol as an extraction solvent, forming an extraneous extrudate water extract by using a certain amount of hydrogenated water as an extraction solvent with respect to the remaining amount of the extruded stone outer leaves, After mixing the above-mentioned extragranular water extracts with a stone-leaf alcohol extract, concentrated under reduced pressure and lyophilized,
Formation of the extralate water extract is carried out at a temperature of 70 to 90 캜,
Wherein the extract from the stalk provides a hangover effect and the aqueous hydrogen peroxide mixed with the stalk extract increases the shelf life of the composition. delete delete delete delete delete