KR101867927B1 - Method for identification of the Genus Pseudomonas - Google Patents

Method for identification of the Genus Pseudomonas Download PDF

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KR101867927B1
KR101867927B1 KR1020150156891A KR20150156891A KR101867927B1 KR 101867927 B1 KR101867927 B1 KR 101867927B1 KR 1020150156891 A KR1020150156891 A KR 1020150156891A KR 20150156891 A KR20150156891 A KR 20150156891A KR 101867927 B1 KR101867927 B1 KR 101867927B1
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dna sequences
polynucleotide consisting
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명인식
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대한민국
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Abstract

The present invention make the plants of 23S rRNA nucleotide hospital bacteria separated from the sample by Pseudomonas Agaricus hour (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali), Pseudomonas Aspergillus you play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae) or Pseudomonas Khanna rain (Pseudomonas cannabina ). When the identification method and the identification probe of the present invention are used, Pseudomonas agaricus ( Pseudomonas sp. agarici , Pseudomonas amygdali), Pseudomonas Aspergillus you play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae ) or Pseudomonas cannabina .

Description

Methods for identification of the genus Pseudomonas in Pseudomonas spp.

The present invention Pseudomonas (Pseudomonas) relates to a method of identifying bacteria belonging to the genus, and more particularly, to determine the base of the 23S rRNA isolated from samples upon Pseudomonas Agaricus (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali), Pseudomonas Aspergillus play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae) or Pseudomonas Khanna rain (Pseudomonas cannabina ).

Dot-blot or polymerase chain reaction (PCR) can be used as a diagnostic method for pathogenic bacteria. In particular, PCR is a widely used method because it is more economical than time, effort or manpower than Dot-blot method. PCR (polymerase chain reaction) is a diagnostic method in which a specific DNA fragment (primer) composed of 10 to 20 bp is annealed to a genomic DNA of a bacterium, followed by heat-resistant DNA polymerase (Taq polymerase) Is added and the synthesis reaction is repeatedly performed, the region to which the primer is bound is rapidly amplified. The PCR amplification product is electrophoresed on an agarose gel or an acrylamide gel, and ethidium bromide DNA staining with ethidium bromide and silver stain, and DNA polymorphism of bacterial species such as differences in the presence or absence of DNA bands. Currently, the PCR method is mainly used to diagnose bacterial diseases in humans and animals. Recently, PCR diagnosis method has been developed for diagnosis of plant pathogens, and is used for quarantine of agricultural products in addition to diagnosis of diseases . In addition, the PCR diagnosis method is shorter in time than other methods, is low in cost for diagnosis, and is also economical because many samples can be assayed at the same time. However, existing PCR identification methods are difficult to classify due to genetic similarity, and a solution to overcome this problem is needed.

By genus Pseudomonas (Pseudomonas) microorganisms include human pathogen P. aeruginosa, the plant pathogen P. syringae, P. fluorescens that promotes plant growth and soil bacterium P. putida known. Among them, Pseudomonas syringae is a rod-shaped gram-negative bacterium with polar flagella, a plant pathogen that can infect a wide variety of plant species and exists in more than 50 hospital types. In particular, Pseudomonas singerger pv. Pseudomonas syringae pv. syringae ) can infect Syringa , Prunus , and Phaseolus plants.

Individual PCR primers used for the identification of some bacteria belonging to the genus Pseudomonas have been developed, but known, when Pseudomonas Agaricus from Pseudomonas species (Pseudomonas) (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali), Pseudomonas Aspergillus you play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae) or Pseudomonas Khanna rain (Pseudomonas cannabina ) have not yet been studied.

Therefore, it is required to develop a method capable of effectively identifying Pseudomonas aeruginosa, Pseudomonas aeruginosa, Pseudomonas asplenia, Pseudomonas avellani or Pseudomonas cannabina through a pathogenic bacterial species or a combination of a pathogenic type specific base and a base.

Object of the present invention, Pseudomonas (Pseudomonas) from Agaricus when Pseudomonas genus (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali , Pseudomonas asplenii , Pseudomonas avellanae , or Pseudomonas cannabina , or a probe for identifying the same.

In order to achieve the above-mentioned technical problem, the present invention provides Pseudomonas Agaricus hour (Pseudomonas agarici , Pseudomonas amygdali), Pseudomonas Aspergillus play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae) or Pseudomonas Khanna rain (Pseudomonas cannabina ). That is, the present invention is to secure the base combination of 23S rRNA genes of Pseudomonas genus Pseudomonas Agaricus hour (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali), Pseudomonas Aspergillus you play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae ) or Pseudomonas cannabina can be identified accurately and quickly.

More specifically, when Pseudomonas Agaricus of the present invention (Pseudomonas agarici) Identification method comprises the steps of separating the 23S rRNA from the sample (a); (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); (C) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 753th base G of SEQ ID NO: 1, the 754th base A of SEQ ID NO: 1, the 1162th base A , Nucleotide 1170 of SEQ ID NO: 1, nucleotide 1173 of SEQ ID NO: 1, nucleotide 1176 of SEQ ID NO: 1, nucleotide 1178 of nucleotide 1178 of SEQ ID NO: 1, nucleotide 1313 of nucleotide 1313 of SEQ ID NO: 1, 1369 to 1363 base GTA of SEQ ID NO: 1, 1397 base G of SEQ ID NO: 1, 1399 base A of SEQ ID NO: 1, 1401 of SEQ ID NO: 1 Base 1420 of SEQ ID NO: 1, base 1440 of base T of SEQ ID NO: 1, nucleotide 1446 of base 14 of SEQ ID NO: 1, nucleotide 1457 of base 1457 of SEQ ID NO: 1, nucleotide C of nucleotide 1563 of SEQ ID NO: 1725 base T of SEQ ID NO: 1, 1730 to 1731 base deletion of SEQ ID NO: 1, nucleotide 1732 of SEQ ID NO: 1 The base G at position 2586 of SEQ ID NO: 1, the base A at position 2587 of SEQ ID NO: 1, the base G at position 2597 of SEQ ID NO: 1, the base A at position 2722 of SEQ ID NO: 1, the base G at position 2739 of SEQ ID NO: 1, And judging it as Pseudomonas agarici when it is the 2740th base T of No. 1.

In addition, Pseudomonas amino of the present invention, unlike the (Pseudomonas amygdali ) identification method comprising the steps of: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is selected from the group consisting of the 1159th base G of SEQ ID NO: 1, the 1370th base deletion of SEQ ID NO: 1, the 1728th base T of SEQ ID NO: 1, the 2256th base T Nucleotide 2262 of SEQ ID NO: 1, nucleotide 2267 of SEQ ID NO: 1, nucleotide 2281 of SEQ ID NO: 1, nucleotide 2286 of SEQ ID NO: 1, nucleotide 2342 of SEQ ID NO: 1, nucleotide G of SEQ ID NO: 1 2358 base deletion and Pseudomonas amygdali in the case of 2549th base C of SEQ ID NO: 1.

Further, in the present invention Pseudomonas Aspergillus play (Pseudomonas asplenii) Identification method comprises the steps of separating the 23S rRNA from the sample (a); (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 135th base T of SEQ ID NO: 1, the 169th base A of SEQ ID NO: 1, the 741th base A of SEQ ID NO: , Base 1032 of base sequence G of SEQ ID NO: 1, base 1034 of base sequence T of SEQ ID NO: 1, base 1035 of base sequence A of SEQ ID NO: 1, base 1170 of base sequence G of SEQ ID NO: 1, base 1173 of base sequence C of SEQ ID NO: 1, Nucleotide 1177 of SEQ ID NO: 1, nucleotide T of nucleotide 1275 of SEQ ID NO: 1, nucleotide 1309 of nucleotide 1309 of SEQ ID NO: 1, nucleotide 1313 of nucleotide T of SEQ ID NO: 1, nucleotide C of nucleotide 1360 of SEQ ID NO: The 1373th base G of SEQ ID NO: 1, the 1440th base T of SEQ ID NO: 1, the 1446th base A of SEQ ID NO: 1, the 1457th base A of SEQ ID NO: 1, the 1469th base A of SEQ ID NO: T base, 1725 to 1727 base CTT of SEQ ID NO: 1, 1729 base C of SEQ ID NO: 1, 1730 to 1731 base of SEQ ID NO: 1 1, nucleotide 2172 of SEQ ID NO: 1, nucleotide 2192 of SEQ ID NO: 1, nucleotide 2238 of SEQ ID NO: 1, nucleotide 2250 of SEQ ID NO: 1, nucleotide A of SEQ ID NO: The 2351th base T of SEQ ID NO: 1, the 2352th base G of SEQ ID NO: 1, the 2361th base C of SEQ ID NO: 1, the 2362nd base T of SEQ ID NO: 1, the 2409th base C of SEQ ID NO: 1 The 2417th base G of SEQ ID NO: 1, the 2447th base A of SEQ ID NO: 1, the 2542th base G of SEQ ID NO: 1, the 2543th base A of SEQ ID NO: 1, the 2545th base T of SEQ ID NO: 1, 2549th base A of SEQ ID NO: 1, 2586th base C of SEQ ID NO: 1, 2587th base A of SEQ ID NO: 1, 2597th base G of SEQ ID NO: 1, 2615th base A of SEQ ID NO: 1, The 2616th base G of SEQ ID NO: 1, the 2618th base C of SEQ ID NO: 1, and the 2794th base A of SEQ ID NO: 1, Nasu Ars play (Pseudomonas asplenii ).

In addition, the present Pseudomonas aeruginosa avellanae) Identification method comprises the steps of separating the 23S rRNA from the sample (a); (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 1159th base G of SEQ ID NO: 1, the 1359th base A of SEQ ID NO: 1, the 1725th base C , Nucleotide 1730 of SEQ ID NO: 1, nucleotide 1732 of SEQ ID NO: 1, nucleotide 2358 of nucleotide 1 of SEQ ID NO: 1, nucleotide 2586 of SEQ ID NO: 1, nucleotide 2587 of SEQ ID NO: 1, nucleotide A of SEQ ID NO: 1 2597th base G, 2722th base A of SEQ ID NO: 1, 2739th base G of SEQ ID NO: 1 and 2740th base T of SEQ ID NO: 1 as Pseudomonas avellanae .

In addition, Pseudomonas Khanna rain of this invention (Pseudomonas cannabina ) comprises the steps of: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 1162th base A of SEQ ID NO: 1, the 1397th base G of SEQ ID NO: 1, the 1725th base C , Nucleotide 1730 of SEQ ID NO: 1, nucleotide 1732 of SEQ ID NO: 1, nucleotide 2358 of SEQ ID NO: 1, nucleotide C of nucleotide 2586 of SEQ ID NO: 1, nucleotide 2587 of SEQ ID NO: 1, nucleotide A of SEQ ID NO: 1 2597th base G of SEQ ID NO: 1, 2722th base A of SEQ ID NO: 1, 2724th base G of SEQ ID NO: 1, 2738-2740th base CGT of SEQ ID NO: 1, and 2755th base of SEQ ID NO: G, and Pseudomonas cannabina .

In another aspect, the present invention when Pseudomonas Agaricus consisting of gene combinations (Pseudomonas agarici , Pseudomonas amygdali), Pseudomonas Aspergillus play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae) or Pseudomonas Khanna rain (Pseudomonas cannabina ) identification probe. That is, the present invention provides Pseudomonas (Pseudomonas) in the 23S rRNA gene in combination to secure the base Pseudomonas Agaricus hour (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali), Pseudomonas Aspergillus you play (Pseudomonas asplenii), Pseudomonas Abel Raney (Pseudomonas avellanae ) or Pseudomonas cannabina .

More specifically, when Pseudomonas Agaricus of the present invention (Pseudomonas agarici ) identifying probe comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 753th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 754th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1162nd nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1170th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1173th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1176th base G of SEQ ID NO: 1, a polynucleotide consisting of the 1178th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences containing SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1313th nucleotide T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1313-1363 bases A polynucleotide consisting of 20-100 consecutive DNA sequences comprising CAC, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1369th nucleotide G of SEQ ID NO: 1, 1371-1373 of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the base GTA, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1397th base G of SEQ ID NO: 1, a polynucleotide consisting of 1399 20-100 consecutive DNA sequences comprising the polynucleotide consisting of 20-100 consecutive DNA sequences comprising the base A, 1401 base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1420th base T of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1440th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1446th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1457th nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1563th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1725th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1727th nucleotide T of SEQ ID NO: 1, a polynucleotide consisting of 1730-1731 nucleotides of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base deletion, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1732th base G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2587th nucleotide A of SEQ ID NO: 1, a polynucleotide consisting of 2097 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2722th base A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, 20-100 consecutive DNA sequences comprising 2740th base T of SEQ ID NO: 1 And complementary polynucleotides thereof. ≪ Desc / Clms Page number 7 >

More specifically, unlike Pseudomonas amino those of the present invention (Pseudomonas amygdali ) identifying probe comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1159th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1370th base deletion of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 1728 of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 2256th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2262nd nucleotide G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2267th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2281th base T of SEQ ID NO: 1, a polynucleotide consisting of the 2286nd nucleotide of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotides T, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2342th nucleotides G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base deletion, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2549th base C of SEQ ID NO: 1, and complementary polynucleotides thereof ≪ / RTI > and at least one polynucleotide selected from the group consisting of < RTI ID = 0.0 >

More specifically, the present invention Pseudomonas Aspergillus play (Pseudomonas asplenii ) identifying probe comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 135th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 169th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 741th nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1032th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1034th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1035th base A of SEQ ID NO: 1, a polynucleotide consisting of the 1170th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1173th nucleotide C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1178th nucleotide C of SEQ ID NO: 1, a polynucleotide consisting of 2075 consecutive DNA sequences comprising the 1275th nucleotide T of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1309th base A of SEQ ID NO: 1, a polynucleotide consisting of 2013 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising 1360th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1363th base C of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising the 1371th nucleotide G of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1440th base T of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 1446th nucleotide A of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1457th base A of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising the 1469th base T of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1725 to 1727 base CTT of SEQ ID NO: 1, a polynucleotide consisting of 1729 base C A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising deletion of nucleotides 1730-1731 of SEQ ID NO: 1, a polynucleotide consisting of 20- G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2182th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2182st nucleotide C of SEQ ID NO: 1, T, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2238th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: A, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, 20-100 consecutive DNA sequences containing the 2311th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2351 base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2352 base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2361th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 2362nd nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2409th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 2417th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2447th base A of SEQ ID NO: 1, a polynucleotide consisting of the 2542th salt of SEQ ID NO: 1 G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2543th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2543th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: T, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2549th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2586th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2586th nucleotide C of SEQ ID NO: 1, A consisting of 20-100 consecutive DNA sequences comprising the polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: Comprises a polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2615th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2616th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2618th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2794th base A of SEQ ID NO: 1 And complementary polynucleotides thereof. The term " polynucleotide "

More specifically, the present Pseudomonas aeruginosa avellanae ) identification probe comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1159th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1359th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1725th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1730th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1732th base deletion of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2358 base T of SEQ ID NO: 1, a polynucleotide consisting of the 2586th nucleotide of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising group C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2587th nucleotide A of SEQ ID NO: 1, a polynucleotide consisting of 2097 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2722th base A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2740th base T of SEQ ID NO: 1, and complementary polynucleotides thereof ≪ / RTI > and at least one polynucleotide selected from the group consisting of < RTI ID = 0.0 >

More particularly, the Pseudomonas Khanna rain of this invention (Pseudomonas cannabina ) identification probe comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1162nd nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1397th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1725th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1730th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1732th base deletion of SEQ ID NO: 1 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2358 base C of SEQ ID NO: 1, a polynucleotide consisting of 2586th nucleotides of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising group C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2587th nucleotide A of SEQ ID NO: 1, a polynucleotide consisting of 2097 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2631th base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base A, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2724th base G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences containing the 2740th base CGT, 20-100 consecutive DNA sequences containing 2755th base G of SEQ ID NO: 1 The polynucleotides to be constructed, and the complementary polynucleotides thereof.

In the present invention, the nucleic acid may be separated in step (a) according to a method commonly used in the art, and may be carried out using a commercially available extraction kit.

Also, the base sequence in step (b) may be performed according to automatic or manual base sequence analysis methods known in the art. Preferably, PCR is performed on a test specimen or a sample to perform PCR corresponding to 23S rRNA And the sequence of the product can be analyzed and analyzed according to a known nucleotide sequence analysis method.

In the step (c), the nucleotide sequence corresponding to the judgment point in the microorganism of the present invention can be identified based on SEQ ID NO: 1 to determine whether the microorganism corresponds to the present invention.

In addition, the present invention Pseudomonas Agaricus hour (Pseudomonas including to effectively identify the respective microorganism, one or more probes for the identification agarici , Pseudomonas amygdali), Pseudomonas Aspergillus play (Pseudomonas asplenii , Pseudomonas avellanae ) or a Pseudomonas cannabina identification kit.

Meanwhile, the present invention provides a microarray for identifying a microorganism of the present invention, wherein the probe of the present invention is integrated on a substrate. The microarray consists of a conventional microarray except that it contains a polynucleotide of the present invention, and a method for producing a microarray by immobilizing a polynucleotide used as a marker on an organ is well known in the art.

In addition, the present invention is a marker for identification of a microorganism of the present invention, wherein a nucleotide having a nucleotide sequence of SEQ ID NO: 1 is substituted for each base corresponding to the judgment point of the microorganism of the present invention Lt; / RTI > The judgment point is the same as that disclosed in the identification method.

It will be apparent to those skilled in the art that the nucleotide, probe or marker of the present invention may be DNA or RNA, and in the case of RNA, the thymine described in the sequence is replaced by uracil.

Of the present invention Pseudomonas Agaricus hour (Pseudomonas agarici), Pseudomonas amido that otherwise (Pseudomonas amygdali), Pseudomonas Aspergillus you play (Pseudomonas asplenii , Pseudomonas avellanae) or Pseudomonas Khanna Herzegovina (Pseudomonas cannabina ) identification method and the identification probe, it is possible to effectively identify each plant pathogenic bacterium from the genus Pseudomonas.

Figure 1 is a graphical representation of Pseudomonas singerger pv. Pseudomonas syringae pv. syringae ) and Pseudomonas agarici ( pseudomonas agarici ).
FIG. 2 shows the Pseudomonas syringer pv. Pseudomonas syringae pv. syringae ) and Pseudomonas amygdali ( Pseudomonas amygdali ).
FIG. 3 is a graphical representation of Pseudomonas syringer pv. Pseudomonas syringae pv. syringae ) and Pseudomonas asplenii (SEQ ID NO: 1 ).
FIG. 4 is a graphical representation of Pseudomonas singerger pv. Pseudomonas syringae pv. syringae ) and Pseudomonas avellanae (hereinafter, referred to as " Pseudomonas avellanae ").
FIG. 5 is a graphical representation of Pseudomonas singerger pv. Pseudomonas syringae pv. syringae ) and / or Pseudomonas cannabina .

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

Experimental Example  One: Pseudomonas  23S of plant pathogenic bacteria rRNA  analysis

Identification probes for each of the Pseudomonas species were designed through the following experimental methods. The total RNA isolation, base sequence amplification and analysis methods used in the present invention are as follows.

<1-1> Pseudomonas 23S of genus bacteria rRNA  Amplification

Pseudomonas agaric , Pseudomonas amygdalia , Pseudomonas asplenii , Pseudomonas avellanae And Pseudomonas cannabina were isolated and PCR was carried out using the primer pairs shown in Table 1 below, which can isolate 23s rRNA of each Pseudomonas sp .

Name of the primer Primer sequence Pseudo23SF1 (forward) 5'-GGTTAGCTCAACGCCTCACA-3 ' Pseudo23SR1 (reverse direction) 5'-GGTGGATGCCCTGGCAG-3 ' Xan23SF7 (forward direction) 5'-GAGACCGCCCCAGTCAAACTAC-3 ' Xan23SR7 (reverse direction) 5'-ACCTTTTGTATAATGGGTCAACG-3 '

The PCR reaction consisted of 20 ng of isolated total DNA, 10 pmoles of downstream primer, 10 pmoles of upstream primer, 0.2 U of Taq DNA polymerase, 10 times Polymerase chain reaction buffer (100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl 2 , pH 8.3) was added and distilled water was added to make 50 μl of the reaction solution.

The reaction solution was heated at 95 ° C. for 5 minutes and then amplified 30 times at 95 ° C. for 30 seconds, 58 ° C. for 60 seconds, and 72 ° C. for 180 seconds. Finally, the reaction solution was treated at 72 ° C. for 10 minutes with a PCR machine (DNA Engine PTC-200) Respectively. After amplification, the PCR product was electrophoresed using 1 × TBE buffer and 1.5% agarose gel, stained with ethidium bromide, and then confirmed by ultraviolet lamp.

<1-2> Amplified 23s rRNA  Sequencing

The nucleotide sequences of the PCR products obtained in Example <1-1> were analyzed using BioEdit Sequence Alignment Editor.

After analysis, each Pseudomonas The 23S rRNA base sequence of the genus Bacillus was identified as Pseudomonas syringae pv. and 23S rRNA sequences of syringae . Pseudomonas syringae pv. The nucleotide sequence of syringae was obtained from Genbank accession number (CP006256). The 23s rRNA sequences of the bacteria used in the present experiment are shown in SEQ ID NOs listed in Table 2 below.

SEQ ID NO: Germ One Pseudomonas syringae pv. syringae 2 Pseudomonas agaric 3 Pseudomonas amygdalium 4 Pseudomonas asplenii 5 Pseudomonas avellanae 6 Pseudomonas cannabina

Experimental Example  2: Pseudomonas  23S of plant pathogenic bacteria rRNA  Sequence alignment

The nucleotide sequences of SEQ ID NOS: 2 to 6 were compared and analyzed based on SEQ ID NO: 1 shown in Table 2 above. The nucleotide sequences were compared using the BioEdit Sequence Alignment Editor.

Pseudomonas The results of the analysis on agarici are shown in Figs. 1A and 1B, and Pseudomonas The nucleotide sequence of the agarici (SEQ ID NO: 2) was Pseudomonas syringae pv. Based on the nucleotide sequence of syringae (SEQ ID NO: 1) 96, 753, 754, 1162, 1170, 1173, 1176, 1178, 1313, 1361 to 1363, 1369, 1371 to 1373, 1397, 1399, 1401, 1420, 1440, G, C, G, C, G, C, G, C, G, and E are located at positions 1, 1446, 1457, 1563, 1725, 1727, 1730 to 1731, 1732, 2586, 2587, 2597, 2722, 2739, G, C, G, A, G, T, A, C, T, T, A, A, C, C, T, have. Using the difference of the bases, Pseudomonas Pseudomonas agarici The strain can be identified.

In addition, Pseudomonas Analysis results for amygdali are shown in Figures 2a and 2b, and Pseudomonas The nucleotide sequence of amygdali (SEQ ID NO: 3) is Pseudomonas syringae pv. G, deletion (A), T, T, G, and G at 1159, 1370, 1728, 2256, 2262, 2267, 2281, 2286, 2342, 2358 and 2549 th positions based on the nucleotide sequence of syringae (SEQ ID NO: G, T, T, G, defect (A), and C base. Using the difference of the bases, Pseudomonas From the genus Pseudomonas amygdali The strain can be identified.

In addition, Pseudomonas Analysis results for asplenii are shown in Figures 3a and 3b, and Pseudomonas The nucleotide sequence of asplenii (SEQ ID NO: 4) is Pseudomonas syringae pv. 1394 , 1360, 1363, 1371, 1440, 1446, 1394, 1360, 1346, 1394, 1370, 1173, 1176, 1178, 1275, 1309, 1313, 1360, 1363, 1457, 1469, 1725 to 1727, 1729, 1730 to 1731, 1732, 2182, 2192, 2238, 2250, 2311, 2351, 2352, 2361, 2362, 2409, 2417, 2447, 2542, 2543, 2545, 2549, 2552, T, A, A, G, T, A, G, C, G, C, T, A, T, C, C, G at positions 2586, 2587, 2597, 2615, 2616, 2618, G, C, T, A, A, T, A, G, C, T, C, G, A, G, A, T, T, A, T, CTT, C, A, C, C, A, G, A, G, C, Using the difference of the bases, Pseudomonas Pseudomonas from the genus asplenii The strain can be identified.

In addition, Pseudomonas The results of analysis for avellanae are shown in FIGS. 4A and 4B, and the nucleotide sequence of Pseudomonas avellanae (SEQ ID NO: 5) is Pseudomonas syringae pv. G, A, C, and G at positions 96, 1159, 1359, 1725, 1730, 1732, 2358, 2586, 2587, 2597, 2722, 2739, and 2740 positions based on the nucleotide sequence of syringae (SEQ ID NO: (A), T, C, A, G, A, G, and T bases are present. Pseudomonas from the genus Pseudomonas, using the difference between the base avellanae The strain can be identified.

In addition, Pseudomonas Analysis results for cannabina are shown in FIGS. 5A and 5B, and the nucleotide sequence of Pseudomonas cannabina (SEQ ID NO: 6) is Pseudomonas syringae pv. 2358, 2586, 2587, 2597, 2631, 2722, 2724, 2738-2740, 2755 positions of 96, 1162, 1397, 1725, 1730, 1732 defects based on the nucleotide sequence of syringae (SEQ ID NO: A, G, C, G, A, C, C, A, G, C, A, G, CGT, Pseudomonas cannabina strains can be identified from the genus Pseudomonas using the difference between the bases.

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Method for identification of the Genus Pseudomonas <130> 2015-0397-10-A / P15-277 <160> 6 <170> Kopatentin 2.0 <210> 1 <211> 2890 <212> DNA <213> Pseudomonas syringae pv_ syringae HS191 23S rRNA <400> 1 tggtcaagcc tcacgggcaa ttagtattgg ttagctcaac gcctcacagc gcttacacac 60 ccaacctatc aacgtcgtag tcttcgacgg ccctttaggg aactcaaggt tccagtgaga 120 7 tctcatcttg aggcaagttt cccgcttaga tgctttcagc ggttatcttt tccgaacata 180 gctacccggc aatgccactg gcgtgacaac cggaacacca gaggttcgtc cactccggtc 240 ctctcgtact aggagcagcc cctctcaaat ctcaaacgtc cacggcagat agggaccgaa 300 ctgtctcacg acgttctaaa cccagctcgc gtaccacttt aaatggcgaa cagccatacc 360 cttgggaccg gcttcagccc caggatgtga tgagccgaca tcgaggtgcc aaacaccgcc 420 gtcgatatga actcttgggc ggtatcagcc tgttatcccc ggagtacctt ttatccgttg 480 agcgatggcc cttccataca gaaccaccgg atcactaaga cctactttcg tacctgctcg 540 acgtgtctgt ctcgcagtca agcgcgcttt tgcctttata ctctacgacc gatttccgac 600 cggtctgagc gcaccttcgt actcctccgt tactctttag gaggagaccg ccccagtcaa 660 actacccacc atacactgtc ctcgatccgg ataacggacc tgagttagaa cctcaaagtt 720 gccagggtgg tatttcaagg ttggctccac gcagactggc gtccacgctt caaagcctcc 780 cacctatcct acacaagcaa attcaaagtc cagtgcaaag ctatagtaaa ggttcacggg 840 gtctttccgt ctagccgcgg atacactgca tcttcacagc gatttcaatt tcactgagtc 900 tcgggtggag acagcgccgc catcgttacg ccattcgtgc aggtcggaac ttacccgaca 960 aggaatttcg ctaccttagg accgttatag ttacggccgc cgtttaccgg ggcttcgatc 1020 aagagcttcg cttgcgctaa ccccatcaat taaccttccg gcaccgggca ggcgtcacac 1080 cctatacgtc cactttcgtg tttgcagagt gctgtgtttt taataaacag tcgcagcggc 1140 ctggtatctt cgaccggcat gggcttacgc agtaaatgct tcaccctcac cggcgcacct 1200 tctcccgaag ttacggtgcc attttgccta gttccttcac ccgagttctc tcaagcgcct 1260 tggtattctc tacccaacca cctgtgtcgg tttggggtac ggttcctggt tacctgaagc 1320 ttagaagctt ttcttggaag catggcatca accacttcgt gttctaaaag aacactcgtc 1380 atcagctctc ggccttaaga tcccggattt acctaagatc tcagcctacc accttaaacc 1440 tggactacca acgccaggct ggcctagcct tctccgtccc tccatcgcaa taaccagaag 1500 tacaggaata ttaacctgtt ttccatcgac tacgcttttc agcctcgcct tagggaccga 1560 ctaaccctgc gtcgattaac gttgcgcagg aaaccttggt ctttcggcgt gggtgttttt 1620 cacacccatt gtcgttactc atgtcagcat tcgcacttct gatacctcca gcaagcttct 1680 caactcacct tcacaggctt acagaacgct cctctaccgc atcatcaaaa gatgataccc 1740 gtagcttcgg tgcatggttt gagccccgtt acatcttccg cgcaggccga ctcgactagt 1800 gagctattac gctttcttta aagggtggct gcttctaagc caacctccta gctgtctaag 1860 ccttcccaca tcgtttccca cttaaccatg actttgggac cttagctgac ggtctgggtt 1920 gtttcccttt tcacgacgga cgttagcacc cgccgtgtgt ctcccatgct cggcacttgt 1980 aggtattcgg agtttgcatc ggtttggtaa gtcgggatga ccccctagcc gaaacagtgc 2040 tctaccccct acagtgatac atgaggcgct acctaaatag ctttcgagga gaaccagcta 2100 tctccgagct tgattagcct ttcactccga tccacaggtc atccgctaac ttttcaacgg 2160 tagtcggttc ggtcctccag ttagtgttac ccaaccttca acctgcccat ggatagatcg 2220 cccggtttcg ggtctattcc cagcgactag acgccctatt aagactcgct ttcgctacgc 2280 ctcccctatt cggttaagct cgccactgaa aataagtcgc tgacccatta tacaaaaggt 2340 acgcagtcac ccaacaaagt gggctcccac tgcttgtacg catacggttt caggatctat 2400 ttcactccgc tctccgcggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2460 cagtcagtag tatttagcct tggaggatgg tccccccata ttcagacaag gtttctcgtg 2520 ccccgtccta ctcgatttca ttgcaaaggg attttcgcgt acagggctat cacccactat 2580 ggccgtcctt tccagaacgt tccgctaatc tcaatacaac ttaagggctg gtccccgttc 2640 gctcgccact actaagggaa tctcggttga tttcttttcc tcagggtact tagatgtttc 2700 agttcccctg gttcgcctct tgcacctatg tattcagtac aagataacca tcttatgatg 2760 gctgggttcc cccattcaga catctccgga tcacagtctg tttgccgact ccccgaagct 2820 tttcgcaggc taccacgtct ttcatcgcct ctgactgcca aggcatccac cgtatgcgct 2880 tcttcacttg 2890 <210> 2 <211> 2767 <212> DNA <213> Pseudomonas agarici 23S rRNA <400> 2 ctatcaacgt cgtagtcttc gacggccctt cagggaactc aaggttccag tgagatctca 60 tcttgaggca agtttcccgc ttagatgctt tcagcggtta tcttttccga acatagctac 120 ccggcaatgc cactggcgtg acaaccggaa caccagaggt tcgtccactc cggtcctctc 180 gtactaggag cagcccctct caaatctcaa acgtccacgg cagataggga ccgaactgtc 240 tcacgacgtt ctaaacccag ctcgcgtacc actttaaatg gcgaacagcc atacccttgg 300 gccggcttc agccccagga tgtgatgagc cgacatcgag gtgccaaaca ccgccgtcga 360 tatgaactct tgggcggtat cagcctgtta tccccggagt accttttatc cgttgagcga 420 tggcccttcc atacagaacc accggatcac taagacctac tttcgtacct gctcgacgtg 480 tctgtctcgc agtcaagcgc gcttttgcct ttatactcta cgaccgattt ccgaccggtc 540 tgagcgcacc ttcgtactcc tccgttactc tttaggagga gaccgcccca gtcaaactac 600 ccaccataca ctgtcctcga tccggataac ggacctgagt tagaacctca aagttgccag 660 ggtggtattt caaggttggc tccacgcgaa ctggcgtcca cgcttcaaag cctcccacct 720 atcctacaca agcaaattca aagtccagtg caaagctata gtaaaggttc acggggtctt 780 tccgtctagc cgcggataca ctgcatcttc acagcgattt caatttcact gagtctcggg 840 tggagacagc gccgccatcg ttacgccatt cgtgcaggtc ggaacttacc cgacaaggaa 900 tttcgctacc ttaggaccgt tatagttacg gccgccgttt accggggctt cgatcaagag 960 cttcgcttgc gctaacccca tcaattaacc ttccggcacc gggcaggcgt cacaccctat 1020 acgtccactt tcgtgtttgc agagtgctgt gtttttaata aacagtcgca gcggcctggt 1080 atcttcgacc ggcatgagct tacggagcaa gtccttcacc ctcaccggcg caccttctcc 1140 cgaagttacg gtgccatttt gcctagttcc ttcacccgag ttctctcaag cgccttggta 1200 ttctctaccc aaccacctgt gtcggtttgg ggtacggttc ctggttatct gaagcttaga 1260 agcttttctt ggaagcatgg catcaaccac ttcgtcacct aaagggtaac tcgtcatcag 1320 ctctcggcct tgaaaccccg gatttaccta agatttcagc ctaccacctt aaacttggac 1380 aaccaacgcc aagctggcct agccttctcc gtccctccat cgcaataacc agaagtacag 1440 gaatattaac ctgttttcca tcgactacgc ttttcagcct cgccttaggg accgactcac 1500 cctgcgtcga ttaacgttgc gcaggaaacc ttggtctttc ggcgtgggtg tttttcacac 1560 ccattgtcgt tactcatgtc agcattcgca cttctgatac ctccagcaag cttctcaact 1620 caccttcaca ggcttacaga acgctcctct accgcatcac ctaagtgata cccgtagctt 1680 cggtgcatgg tttgagcccc gttacatctt ccgcgcaggc cgactcgact agtgagctat 1740 tacgctttct ttaaagggtg gctgcttcta agccaacctc ctagctgtct aagccttccc 1800 acatcgtttc ccacttaacc atgactttgg gaccttagct gacggtctgg gttgtttccc 1860 ttttcacgac ggacgttagc acccgccgtg tgtctcccat gctcggcact tgtaggtatt 1920 cggagtttgc atcggtttgg taagtcggga tgacccccta gccgaaacag tgctctaccc 1980 cctacagtga tacatgaggc gctacctaaa tagctttcga ggagaaccag ctatctccga 2040 gcttgattag cctttcactc cgatccacag gtcatccgct aacttttcaa cggtagtcgg 2100 ttcggtcctc cagttagtgt tacccaacct tcaacctgcc catggataga tcgcccggtt 2160 tcgggtctat tcccagcgac tagacgccct attaagactc gctttcgcta cgcctcccct 2220 attcggttaa gctcgccact gaaaataagt cgctgaccca ttatacaaaa ggtacgcagt 2280 cacccaacaa agtgggctcc cactgcttgt acgcatacgg tttcaggatc tatttcactc 2340 cgctctccgc ggttcttttc gcctttccct cacggtactg gttcactatc ggtcagtcag 2400 tagtatttag ccttggagga tggtcccccc atattcagac aaggtttctc gtgccccgtc 2460 ctactcgatt tcattgcaaa gggattttcg cgtacagggc tatcacccac tatggccgca 2520 ctttccagag cgttccgcta atctcaatac aacttaaggg ctggtccccg ttcgctcgcc 2580 actactaagg gaatctcggt tgatttcttt tcctcagggt acttagatgt ttcagttccc 2640 ctggttcgcc tcttacacct atgtattcag tgtaagataa ccatcttatg atggctgggt 2700 tcccccattc agacatctcc ggatcacagt ctgtttgccg actccccgaa gcttttcgca 2760 ggctacc 2767 <210> 3 <211> 2762 <212> DNA <213> Pseudomonas amygdali 23S rRNA <400> 3 ctatcaacgt cgtagtcttc gacggccctt tagggaactc aaggttccag tgagatctca 60 tcttgaggca agtttcccgc ttagatgctt tcagcggtta tcttttccga acatagctac 120 ccggcaatgc cactggcgtg acaaccggaa caccagaggt tcgtccactc cggtcctctc 180 gtactaggag cagcccctct caaatctcaa acgtccacgg cagataggga ccgaactgtc 240 tcacgacgtt ctaaacccag ctcgcgtacc actttaaatg gcgaacagcc atacccttgg 300 gccggcttc agccccagga tgtgatgagc cgacatcgag gtgccaaaca ccgccgtcga 360 tatgaactct tgggcggtat cagcctgtta tccccggagt accttttatc cgttgagcga 420 tggcccttcc atacagaacc accggatcac taagacctac tttcgtacct gctcgacgtg 480 tctgtctcgc agtcaagcgc gcttttgcct ttatactcta cgaccgattt ccgaccggtc 540 tgagcgcacc ttcgtactcc tccgttactc tttaggagga gaccgcccca gtcaaactac 600 ccaccataca ctgtcctcga tccggataac ggacctgagt tagaacctca aagttgccag 660 ggtggtattt caaggttggc tccacgcaga ctggcgtcca cgcttcaaag cctcccacct 720 atcctacaca agcaaattca aagtccagtg caaagctata gtaaaggttc acggggtctt 780 tccgtctagc cgcggataca ctgcatcttc acagcgattt caatttcact gagtctcggg 840 tggagacagc gccgccatcg ttacgccatt cgtgcaggtc ggaacttacc cgacaaggaa 900 tttcgctacc ttaggaccgt tatagttacg gccgccgttt accggggctt cgatcaagag 960 cttcgcttgc gctaacccca tcaattaacc ttccggcacc gggcaggcgt cacaccctat 1020 acgtccactt tcgtgtttgc agagtgctgt gtttttaata aacagtcgca gcggcctggt 1080 atcttcgacc ggcgtgggct tacgcagtaa atgcttcacc ctcaccggcg caccttctcc 1140 cgaagttacg gtgccatttt gcctagttcc ttcacccgag ttctctcaag cgccttggta 1200 ttctctaccc aaccacctgt gtcggtttgg ggtacggttc ctggttacct gaagcttaga 1260 agcttttctt ggaagcatgg catcaaccac ttcgtgttct aaagaacact cgtcatcagc 1320 tctcggcctt aagatcccgg atttacctaa gatctcagcc taccacctta aacctggaca 1380 accaacgcca ggctggccta gccttctccg tccctccatc gcaataacca gaagtacagg 1440 aatattaacc tgttttccat cgactacgct tttcagcctc gccttaggga ccgactaacc 1500 ctgcgtcgat taacgttgcg caggaaacct tggtctttcg gcgtgggtgt ttttcacacc 1560 cattgtcgtt actcatgtca gcattcgcac ttctgatacc tccagcaagc ttctcaactc 1620 accttcacag gcttacagaa cgctcctcta ccgcatcatc ataagatgat acccgtagct 1680 tcggtgcatg gtttgagccc cgttacatct tccgcgcagg ccgactcgac tagtgagcta 1740 ttacgctttc tttaaagggt ggctgcttct aagccaacct cctagctgtc taagccttcc 1800 cacatcgttt cccacttaac catgactttg ggaccttagc tgacggtctg ggttgtttcc 1860 cttttcacga cggacgttag cacccgccgt gtgtctccca tgctcggcac ttgtaggtat 1920 tcggagtttg catcggtttg gtaagtcggg atgaccccct agccgaaaca gtgctctacc 1980 ccctacagtg atacatgagg cgctacctaa atagctttcg aggagaacca gctatctccg 2040 agcttgatta gcctttcact ccgatccaca ggtcatccgc taacttttca acggtagtcg 2100 gttcggtcct ccagttagtg ttacccaacc ttcaacctgc ccatggatag atcgcccggt 2160 ttcgggtcta ttcccagcga ctagacgcct tattaggact ggctttcgct acgcttccct 2220 tattcggtta agctcgccac tgaaaataag tcgctgaccc attatacaaa aggtaggcag 2280 tcacccaaca agtgggctcc cactgcttgt acgcatacgg tttcaggatc tatttcactc 2340 cgctctccgc ggttcttttc gcctttccct cacggtactg gttcactatc ggtcagtcag 2400 tagtatttag ccttggagga tggtcccccc atattcagac aaggtttctc gtgccccgtc 2460 ctactcgatt tcattgcaaa gcgattttcg cgtacagggc tatcacccac tatggccgtc 2520 ccttccagaa cgttccgcta atctcaatac aacttaaggg ctggtccccg ttcgctcgcc 2580 actactaagg gaatctcggt tgatttcttt tcctcagggt acttagatgt ttcagttccc 2640 ctggttcgcc tcttgcacct atgtattcag tacaagataa ccatcttatg atggctgggt 2700 tcccccattc agacatctcc ggatcacagt ctgtttgccg actccccgaa gcttttcgca 2760 gg 2762 <210> 4 <211> 2778 <212> DNA <213> Pseudomonas asplenii 23S rRNA <400> 4 ctatcaacgt cgtagtcttc gacggccctt cagggaactc aaggttccag tgagatctca 60 tcttgaggct agtttcccgc ttagatgctt tcagcggtta tctattccga acatagctac 120 ccggcaatgc cactggcgtg acaaccggaa caccagaggt tcgtccactc cggtcctctc 180 gtactaggag cagcccctct caaatctcaa acgtccacgg cagataggga ccgaactgtc 240 tcacgacgtt ctaaacccag ctcgcgtacc actttaaatg gcgaacagcc atacccttgg 300 gccggcttc agccccagga tgtgatgagc cgacatcgag gtgccaaaca ccgccgtcga 360 tatgaactct tgggcggtat cagcctgtta tccccggagt accttttatc cgttgagcga 420 tggcccttcc atacagaacc accggatcac taagacctac tttcgtacct gctcgacgtg 480 tctgtctcgc agtcaagcgc gcttttgcct ttatactcta cgaccgattt ccgaccggtc 540 tgagcgcacc ttcgtactcc tccgttactc tttaggagga gaccgcccca gtcaaactac 600 ccaccataca ctgtcctcga tccggataac ggacctgagt tagaacctca aagttgccag 660 ggtggtattt caaggatggc tccacgcaga ctggcgtcca cgcttcaaag cctcccacct 720 atcctacaca agcaaattca aagtccagtg caaagctata gtaaaggttc acggggtctt 780 tccgtctagc cgcggataca ctgcatcttc acagcgattt caatttcact gagtctcggg 840 tggagacagc gccgccatcg ttacgccatt cgtgcaggtc ggaacttacc cgacaaggaa 900 tttcgctacc ttaggaccgt tatagttacg gccgccgttt accggggctt cgatcaagag 960 cttcgcgtta gctaacccca tcaattaacc ttccggcacc gggcaggcgt cacaccctat 1020 acgtccactt tcgtgtttgc agagtgctgt gtttttaata aacagtcgca gcggcctggt 1080 atcttcgacc ggcatgggct tacggagcaa gtccttcacc ctcaccggcg caccttctcc 1140 cgaagttacg gtgccatttt gcctagttcc ttcacccgag ttctctcaag cgccttggta 1200 ttctctacct aaccacctgt gtcggtttgg ggtacggttc ctgattatct gaagcttaga 1260 agcttttctt ggaagcatgg catcaaccac ttcgcgtcct aaaaggacac tcgtcatcag 1320 ctctcggcct taagatcccg gatttaccta agatctcagc ctaccacctt aaacttggac 1380 aaccaacgcc aagctggcct agctttctcc gtccctccat cgcaataacc agaagtacag 1440 gaatattaac ctgttttcca tcgactacgc ttttcagcct cgccttaggg accgactaac 1500 cctgcgtcga ttaacgttgc gcaggaaacc ttggtctttc ggcgtgggtg tttttcacac 1560 ccattgtcgt tactcatgtc agcattcgca cttctgatac ctccagcaag cttctcaact 1620 caccttcaca ggcttacaga acgctcctct accgcatcac ttacgtgata cccgtagctt 1680 cggtgcatgg tttgagcccc gttacatctt ccgcgcaggc cgactcgact agtgagctat 1740 tacgctttct ttaaagggtg gctgcttcta agccaacctc ctagctgtct aagccttccc 1800 acatcgtttc ccacttaacc atgactttgg gaccttagct gacggtctgg gttgtttccc 1860 ttttcacgac ggacgttagc acccgccgtg tgtctcccat gctcggcact tgtaggtatt 1920 cggagtttgc atcggtttgg taagtcggga tgacccccta gccgaaacag tgctctaccc 1980 cctacagtga tacatgaggc gctacctaaa tagctttcga ggagaaccag ctatctccga 2040 gcttgattag cctttcactc cgatccacag gtcatccgct aacttttcaa cggtagtcgg 2100 ttcggtcctc cagtcagtgt tacctaacct tcaacctgcc catggataga tcgcccggtt 2160 tcgggtctat acccagcgac taaacgccct attaagactc gctttcgcta cgcctcccct 2220 attcggttaa gctcgccact gaatataagt cgctgaccca ttatacaaaa ggtacgcagt 2280 cacagaacaa agtctgctcc cactgcttgt acgcatacgg tttcaggatc tatttcactc 2340 ccctctccgg ggttcttttc gcctttccct cacggtacta gttcactatc ggtcagtcag 2400 tagtatttag ccttggagga tggtcccccc atattcagac aaggtttctc gtgccccgtc 2460 ctactcgatt tcatgactaa gagactttcg cgtacagggc tatcacccac tatggccgca 2520 ctttccagag cgttccgcta atctcaaagc cacttaaggg ctggtccccg ttcgctcgcc 2580 actactaagg gaatctcggt tgatttcttt tcctcagggt acttagatgt ttcagttccc 2640 ctggttcgcc tcttgcacct atgtattcag tacaagataa ccatcttatg atggctgggt 2700 tcccccattc agacatctcc ggatcaaagt ctgtttgccg actccccgaa gctttcgcag 2760 gctacccacg ccacgcca 2778 <210> 5 <211> 2766 <212> DNA <213> Pseudomonas avellanae 23S rRNA <400> 5 ctatcaacgt cgtagtcttc gacggccctt cagggaactc aaggttccag tgagatctca 60 tcttgaggca agtttcccgc ttagatgctt tcagcggtta tcttttccga acatagctac 120 ccggcaatgc cactggcgtg acaaccggaa caccagaggt tcgtccactc cggtcctctc 180 gtactaggag cagcccctct caaatctcaa acgtccacgg cagataggga ccgaactgtc 240 tcacgacgtt ctaaacccag ctcgcgtacc actttaaatg gcgaacagcc atacccttgg 300 gccggcttc agccccagga tgtgatgagc cgacatcgag gtgccaaaca ccgccgtcga 360 tatgaactct tgggcggtat cagcctgtta tccccggagt accttttatc cgttgagcga 420 tggcccttcc atacagaacc accggatcac taagacctac tttcgtacct gctcgacgtg 480 tctgtctcgc agtcaagcgc gcttttgcct ttatactcta cgaccgattt ccgaccggtc 540 tgagcgcacc ttcgtactcc tccgttactc tttaggagga gaccgcccca gtcaaactac 600 ccaccataca ctgtcctcga tccggataac ggacctgagt tagaacctca aagttgccag 660 ggtggtattt caaggttggc tccacgcaga ctggcgtcca cgcttcaaag cctcccacct 720 atcctacaca agcaaattca aagtccagtg caaagctata gtaaaggttc acggggtctt 780 tccgtctagc cgcggataca ctgcatcttc acagcgattt caatttcact gagtctcggg 840 tggagacagc gccgccatcg ttacgccatt cgtgcaggtc ggaacttacc cgacaaggaa 900 tttcgctacc ttaggaccgt tatagttacg gccgccgttt accggggctt cgatcaagag 960 cttcgcttgc gctaacccca tcaattaacc ttccggcacc gggcaggcgt cacaccctat 1020 acgtccactt tcgtgtttgc agagtgctgt gtttttaata aacagtcgca gcggcctggt 1080 atcttcgacc ggcgtgggct tacgcagtaa atgcttcacc ctcaccggcg caccttctcc 1140 cgaagttacg gtgccatttt gcctagttcc ttcacccgag ttctctcaag cgccttggta 1200 ttctctaccc aaccacctgt gtcggtttgg ggtacggttc ctggttacct gaagcttaga 1260 agcttttctt ggaagcatgg catcaaccac ttcatgttct aaaagaacac tcgtcatcag 1320 ctctcggcct taagatcccg gatttaccta agatctcagc ctaccacctt aaacctggac 1380 taccaacgcc aggctggcct agccttctcc gtccctccat cgcaataacc agaagtacag 1440 gaatattaac ctgttttcca tcgactacgc ttttcagcct cgccttaggg accgactaac 1500 cctgcgtcga ttaacgttgc gcaggaaacc ttggtctttc ggcgtgggtg tttttcacac 1560 ccattgtcgt tactcatgtc agcattcgca cttctgatac ctccagcaag cttctcaact 1620 caccttcaca ggcttacaga acgctcctct accgcatcac caaaggtgat acccgtagct 1680 tcggtgcatg gtttgagccc cgttacatct tccgcgcagg ccgactcgac tagtgagcta 1740 ttacgctttc tttaaagggt ggctgcttct aagccaacct cctagctgtc taagccttcc 1800 cacatcgttt cccacttaac catgactttg ggaccttagc tgacggtctg ggttgtttcc 1860 cttttcacga cggacgttag cacccgccgt gtgtctccca tgctcggcac ttgtaggtat 1920 tcggagtttg catcggtttg gtaagtcggg atgaccccct agccgaaaca gtgctctacc 1980 ccctacagtg atacatgagg cgctacctaa atagctttcg aggagaacca gctatctccg 2040 agcttgatta gcctttcact ccgatccaca ggtcatccgc taacttttca acggtagtcg 2100 gttcggtcct ccagttagtg ttacccaacc ttcaacctgc ccatggatag atcgcccggt 2160 ttcgggtcta ttcccagcga ctagacgccc tattaagact cgctttcgct acgcctcccc 2220 tattcggtta agctcgccac tgaaaataag tcgctgaccc attatacaaa aggtacgcag 2280 tcacccaaca atgtgggctc ccactgcttg tacgcatacg gtttcaggat ctatttcact 2340 ccgctctccg cggttctttt cgcctttccc tcacggtact ggttcactat cggtcagtca 2400 gtagtattta gccttggagg atggtccccc catattcaga caaggtttct cgtgccccgt 2460 cctactcgat ttcattgcaa agggattttc gcgtacaggg ctatcaccca ctatggccgc 2520 actttccaga gcgttccgct aatctcaata caacttaagg gctggtcccc gttcgctcgc 2580 cactactaag ggaatctcgg ttgatttctt ttcctcaggg tacttagatg tttcagttcc 2640 cctggttcgc ctcttacacc tatgtattca gtgtaagata accatcttat gatggctggg 2700 ttcccccatt cagacatctc cggatcacag tctgtttgcc gactccccga agcttttcgc 2760 aggcta 2766 <210> 6 <211> 2767 <212> DNA <213> Pseudomonas cannabina 23S rRNA <400> 6 ctatcaacgt cgtagtcttc gacggccctt cagggaactc aaggttccag tgagatctca 60 tcttgaggca agtttcccgc ttagatgctt tcagcggtta tcttttccga acatagctac 120 ccggcaatgc cactggcgtg acaaccggaa caccagaggt tcgtccactc cggtcctctc 180 gtactaggag cagcccctct caaatctcaa acgtccacgg cagataggga ccgaactgtc 240 tcacgacgtt ctaaacccag ctcgcgtacc actttaaatg gcgaacagcc atacccttgg 300 gccggcttc agccccagga tgtgatgagc cgacatcgag gtgccaaaca ccgccgtcga 360 tatgaactct tgggcggtat cagcctgtta tccccggagt accttttatc cgttgagcga 420 tggcccttcc atacagaacc accggatcac taagacctac tttcgtacct gctcgacgtg 480 tctgtctcgc agtcaagcgc gcttttgcct ttatactcta cgaccgattt ccgaccggtc 540 tgagcgcacc ttcgtactcc tccgttactc tttaggagga gaccgcccca gtcaaactac 600 ccaccataca ctgtcctcga tccggataac ggacctgagt tagaacctca aagttgccag 660 ggtggtattt caaggttggc tccacgcaga ctggcgtcca cgcttcaaag cctcccacct 720 atcctacaca agcaaattca aagtccagtg caaagctata gtaaaggttc acggggtctt 780 tccgtctagc cgcggataca ctgcatcttc acagcgattt caatttcact gagtctcggg 840 tggagacagc gccgccatcg ttacgccatt cgtgcaggtc ggaacttacc cgacaaggaa 900 tttcgctacc ttaggaccgt tatagttacg gccgccgttt accggggctt cgatcaagag 960 cttcgcttgc gctaacccca tcaattaacc ttccggcacc gggcaggcgt cacaccctat 1020 acgtccactt tcgtgtttgc agagtgctgt gtttttaata aacagtcgca gcggcctggt 1080 atcttcgacc ggcatgagct tacgcagtaa atgcttcacc ctcaccggcg caccttctcc 1140 cgaagttacg gtgccatttt gcctagttcc ttcacccgag ttctctcaag cgccttggta 1200 ttctctaccc aaccacctgt gtcggtttgg ggtacggttc ctggttacct gaagcttaga 1260 agcttttctt ggaagcatgg catcaaccac ttcgtgttct aaaagaacac tcgtcatcag 1320 ctctcggcct tgagatcccg gatttaccta agatctcagc ctaccacctt aaacctggac 1380 taccaacgcc aggctggcct agccttctcc gtccctccat cgcaataacc agaagtacag 1440 gaatattaac ctgttttcca tcgactacgc ttttcagcct cgccttaggg accgactaac 1500 cctgcgtcga ttaacgttgc gcaggaaacc ttggtctttc ggcgtgggtg tttttcacac 1560 ccattgtcgt tactcatgtc agcattcgca cttctgatac ctccagcaag cttctcaact 1620 caccttcaca ggcttacaga acgctcctct accgcatcac caaaggtgat acccgtagct 1680 tcggtgcatg gtttgagccc cgttacatct tccgcgcagg ccgactcgac tagtgagcta 1740 ttacgctttc tttaaagggt ggctgcttct aagccaacct cctagctgtc taagccttcc 1800 cacatcgttt cccacttaac catgactttg ggaccttagc tgacggtctg ggttgtttcc 1860 cttttcacga cggacgttag cacccgccgt gtgtctccca tgctcggcac ttgtaggtat 1920 tcggagtttg catcggtttg gtaagtcggg atgaccccct agccgaaaca gtgctctacc 1980 ccctacagtg atacatgagg cgctacctaa atagctttcg aggagaacca gctatctccg 2040 agcttgatta gcctttcact ccgatccaca ggtcatccgc taacttttca acggtagtcg 2100 gttcggtcct ccagttagtg ttacccaacc ttcaacctgc ccatggatag atcgcccggt 2160 ttcgggtcta ttcccagcga ctagacgccc tattaagact cgctttcgct acgcctcccc 2220 tattcggtta agctcgccac tgaaaataag tcgctgaccc attatacaaa aggtacgcag 2280 tcacccaaca acgtgggctc ccactgcttg tacgcatacg gtttcaggat ctatttcact 2340 ccgctctccg cggttctttt cgcctttccc tcacggtact ggttcactat cggtcagtca 2400 gtagtattta gccttggagg atggtccccc catattcaga caaggtttct cgtgccccgt 2460 cctactcgat ttcattgcaa agggattttc gcgtacaggg ctatcaccca ctatggccgc 2520 actttccaga gcgttccgct aatctcaata caacttaagg gctgctcccc gttcgctcgc 2580 cactactaag ggaatctcgg ttgatttctt ttcctcaggg tacttagatg tttcagttcc 2640 cctggttcgc ctcttacgcc tatgtattca gcgtaagata accatcttgt gatggctggg 2700 ttcccccatt cagacatctc cggatcacag tctgtttgcc gactccccga agcttttcgc 2760 aggctac 2767

Claims (20)

(a) separating 23S rRNA from the sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); (C) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 753th base G of SEQ ID NO: 1, the 754th base A of SEQ ID NO: 1, the 1162th base A , Nucleotide 1170 of SEQ ID NO: 1, nucleotide 1173 of SEQ ID NO: 1, nucleotide 1176 of SEQ ID NO: 1, nucleotide 1178 of nucleotide 1178 of SEQ ID NO: 1, nucleotide 1313 of nucleotide 1313 of SEQ ID NO: 1, 1369 to 1363 base GTA of SEQ ID NO: 1, 1397 base G of SEQ ID NO: 1, 1399 base A of SEQ ID NO: 1, 1401 of SEQ ID NO: 1 Base 1420 of SEQ ID NO: 1, base 1440 of base T of SEQ ID NO: 1, nucleotide 1446 of base 14 of SEQ ID NO: 1, nucleotide 1457 of base 1457 of SEQ ID NO: 1, nucleotide C of nucleotide 1563 of SEQ ID NO: 1725 base T of SEQ ID NO: 1, 1730 to 1731 base deletion of SEQ ID NO: 1, nucleotide 1732 of SEQ ID NO: 1 The base G at position 2586 of SEQ ID NO: 1, the base A at position 2587 of SEQ ID NO: 1, the base G at position 2597 of SEQ ID NO: 1, the base A at position 2722 of SEQ ID NO: 1, the base G at position 2739 of SEQ ID NO: 1, And determining that the Pseudomonas agarici is the 2740th base T of SEQ ID NO: 1. (a) separating 23S rRNA from the sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is selected from the group consisting of the 1159th base G of SEQ ID NO: 1, the 1370th base deletion of SEQ ID NO: 1, the 1728th base T of SEQ ID NO: 1, the 2256th base T Nucleotide 2262 of SEQ ID NO: 1, nucleotide 2267 of SEQ ID NO: 1, nucleotide 2281 of SEQ ID NO: 1, nucleotide 2286 of SEQ ID NO: 1, nucleotide 2342 of SEQ ID NO: 1, nucleotide G of SEQ ID NO: 1 2358 base deletion and the 2549th base C of SEQ ID NO: 1, Pseudomonas sp. amygdali ). &lt; / RTI &gt; (a) separating 23S rRNA from the sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 135th base T of SEQ ID NO: 1, the 169th base A of SEQ ID NO: 1, the 741th base A of SEQ ID NO: , Base 1032 of base sequence G of SEQ ID NO: 1, base 1034 of base sequence T of SEQ ID NO: 1, base 1035 of base sequence A of SEQ ID NO: 1, base 1170 of base sequence G of SEQ ID NO: 1, base 1173 of base sequence C of SEQ ID NO: 1, Nucleotide 1177 of SEQ ID NO: 1, nucleotide T of nucleotide 1275 of SEQ ID NO: 1, nucleotide 1309 of nucleotide 1309 of SEQ ID NO: 1, nucleotide 1313 of nucleotide T of SEQ ID NO: 1, nucleotide C of nucleotide 1360 of SEQ ID NO: The 1373th base G of SEQ ID NO: 1, the 1440th base T of SEQ ID NO: 1, the 1446th base A of SEQ ID NO: 1, the 1457th base A of SEQ ID NO: 1, the 1469th base A of SEQ ID NO: T base, 1725 to 1727 base CTT of SEQ ID NO: 1, 1729 base C of SEQ ID NO: 1, 1730 to 1731 base of SEQ ID NO: 1 1, nucleotide 2172 of SEQ ID NO: 1, nucleotide 2192 of SEQ ID NO: 1, nucleotide 2238 of SEQ ID NO: 1, nucleotide 2250 of SEQ ID NO: 1, nucleotide A of SEQ ID NO: The 2351th base T of SEQ ID NO: 1, the 2352th base G of SEQ ID NO: 1, the 2361th base C of SEQ ID NO: 1, the 2362nd base T of SEQ ID NO: 1, the 2409th base C of SEQ ID NO: 1 The 2417th base G of SEQ ID NO: 1, the 2447th base A of SEQ ID NO: 1, the 2542th base G of SEQ ID NO: 1, the 2543th base A of SEQ ID NO: 1, the 2545th base T of SEQ ID NO: 1, 2549th base A of SEQ ID NO: 1, 2586th base C of SEQ ID NO: 1, 2587th base A of SEQ ID NO: 1, 2597th base G of SEQ ID NO: 1, 2615th base A of SEQ ID NO: 1, The 2616th base G of SEQ ID NO: 1, the 2618th base C of SEQ ID NO: 1, and the 2794th base A of SEQ ID NO: 1, Nasu Ars you play (Pseudomonas asplenii) a determination step Pseudomonas Ars play your way to identify containing. (a) separating 23S rRNA from the sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 1159th base G of SEQ ID NO: 1, the 1359th base A of SEQ ID NO: 1, the 1725th base C , Nucleotide 1730 of SEQ ID NO: 1, nucleotide 1732 of SEQ ID NO: 1, nucleotide 2358 of nucleotide 1 of SEQ ID NO: 1, nucleotide 2586 of SEQ ID NO: 1, nucleotide 2587 of SEQ ID NO: 1, nucleotide A of SEQ ID NO: 1 2597th base G, the 2722th base A of SEQ ID NO: 1, the 2739th base G of SEQ ID NO: 1 and the 2740th base T of SEQ ID NO: 1 as Pseudomonas avellanae , Identification method. (a) separating 23S rRNA from the sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 96th base C of SEQ ID NO: 1, the 1162th base A of SEQ ID NO: 1, the 1397th base G of SEQ ID NO: 1, the 1725th base C , Nucleotide 1730 of SEQ ID NO: 1, nucleotide 1732 of SEQ ID NO: 1, nucleotide 2358 of SEQ ID NO: 1, nucleotide 2586 of SEQ ID NO: 1, nucleotide 2587 of SEQ ID NO: 1, nucleotide A of SEQ ID NO: 1 2597th base G of SEQ ID NO: 1, 2722th base A of SEQ ID NO: 1, 2724th base G of SEQ ID NO: 1, 2738-2740th base CGT of SEQ ID NO: 1, and 2755th base of SEQ ID NO: If G is Pseudomonas Khanna Herzegovina (Pseudomonas cannabina ). &lt; / RTI &gt; A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 753th base G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 754th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1162nd nucleotide A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1170th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1173th base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1176th base G of SEQ ID NO: 1, 20-100 consecutive sequences comprising the 1178th base C of SEQ ID NO: A polynucleotide consisting of 20-100 contiguous DNA sequences comprising the 1313 base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 contiguous DNA sequences consisting of 1361-1363 bases CAC of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1369th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 1371-1373th base GTA of SEQ ID NO: 1 A polynucleotide consisting of -100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1397th base G of SEQ ID NO: 1, a polynucleotide consisting of 1399th base A of SEQ ID NO: Polynucleotides consisting of 20-100 consecutive DNA sequences comprising a polynucleotide consisting of -100 consecutive DNA sequences, 1401 base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1420th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1440th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1446th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1457th nucleotide A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1563th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1725th base C of SEQ ID NO: 1, Nucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 1727 of SEQ ID NO: 1, a polynucleotide consisting of 1730 to 1731 base deletions of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1732th base G of SEQ ID NO: 1, 2586th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2587th nucleotide A of SEQ ID NO: 1, a polynucleotide consisting of 2597th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2722th base A of SEQ ID NO: 1, a polynucleotide consisting of 2739th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences and 20-100 consecutive DNA sequences comprising 2740th base T of SEQ ID NO: O lactide and their complementary polynucleotide Pseudomonas Agaricus hour (Pseudomonas agarici) identifying probe comprising a. A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1159th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1370th base deletion of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1728th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2256th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2262nd nucleotide G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2267th nucleotide G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 2281 of SEQ ID NO: 1, 20-100 nucleotides comprising the nucleotide 2286 of SEQ ID NO: 1 A polynucleotide consisting of a consecutive DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2342nd nucleotide G of SEQ ID NO: 1, 20-100 nucleotides comprising the 2358th nucleotide deletion of SEQ ID NO: 1 A polynucleotide consisting of a contiguous DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2549th base C of SEQ ID NO: 1, and a complementary polynucleotide of Pseudomonas amygdali) identification probe. A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 135th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 169th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 741st nucleotide A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1032th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1034th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1035 base A of SEQ ID NO: 1, 20-100 consecutive sequences comprising 1170th base G of SEQ ID NO: 1 , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1173 base C of SEQ ID NO: 1, 20-100 consecutive sequences comprising the 1176th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 1178 of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1275th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1309th base A of SEQ ID NO: 1, 20-100 consecutive sequences comprising the 1313th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1360th base C of SEQ ID NO: 1, a polynucleotide consisting of the nucleotide sequence SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences containing 1363th base C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1371th nucleotide G of SEQ ID NO: 1, 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1440th base T, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1446th base A of SEQ ID NO: 1, 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1457th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1469th nucleotide T of SEQ ID NO: 1, 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1725 to 1727 base CTTs, 20-100 nucleotides comprising nucleotide 1729 of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising deletions from nucleotides 1730 to 1731 of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1732 to 1731 base deletions of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2182nd nucleotide C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 21st nucleotide C of SEQ ID NO: 1, 20-100 A polynucleotide consisting of 20-100 contiguous DNA sequences of SEQ ID NO: 1, a polynucleotide consisting of 20-100 contiguous DNA sequences comprising 2238th nucleotide A of SEQ ID NO: 1, 20-100 A polynucleotide consisting of 20-100 contiguous DNA sequences comprising the 2311th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2351th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2352th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2361th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2362nd nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2409th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2417th nucleotide G of SEQ ID NO: 1 , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2447th base A of SEQ ID NO: 1, 20-100 nucleotides comprising 2542th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of a consecutive DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2543th base A of SEQ ID NO: 1, 20-100 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2549th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2552th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 contiguous DNA sequences comprising the 2586th base C of SEQ ID NO: 1, a polynucleotide consisting of 25-100 contiguous DNA sequences of SEQ ID NO: 1, 20-100 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2597th base G of SEQ ID NO: 1, a polynucleotide consisting of a consecutive DNA sequence, A polynucleotide consisting of 20-100 consecutive DNA sequences containing the 2615th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2616th base G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2618th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2794th base A of SEQ ID NO: 1, and A probe for identification of Pseudomonas asplenii comprising complementary polynucleotides thereof. A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1159th base G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1359th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1725th base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1730th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1732th base deletion of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2358 base T of SEQ ID NO: 1, 20-100 nucleotides comprising the 2586th base C of SEQ ID NO: 1 A polynucleotide consisting of a contiguous DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2587th nucleotide A of SEQ ID NO: 1, 20-100 nucleotides comprising 2597th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of a consecutive DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2722th base A of SEQ ID NO: 1, 20-100 of 2739th bases G of SEQ ID NO: 1 A polynucleotide consisting of a consecutive DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2740th base T of SEQ ID NO: 1, and a complementary polynucleotide thereof, Pseudomonas avellanae Identification probe. A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 96th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1162nd nucleotide A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1397th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1725th base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1730th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1732th base deletion of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 2358 of SEQ ID NO: 1, 20-100 nucleotides comprising the 2586th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of a contiguous DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2587th nucleotide A of SEQ ID NO: 1, 20-100 nucleotides comprising 2597th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2631 base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences of SEQ ID NO: 1, A polynucleotide consisting of a consecutive DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2724th base G of SEQ ID NO: 1, a polynucleotide consisting of 2738-2740th base CGT of SEQ ID NO: 1, A polynucleotide consisting of 100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences containing 2755th base G of SEQ ID NO: 1 A probe for identification of Pseudomonas cannabina comprising a polynucleotide and a complementary polynucleotide thereof. A microarray for identification of Pseudomonas agarici , wherein the probe of claim 6 is integrated on a substrate. A microarray for identification of Pseudomonas amygdali , characterized in that the probe of claim 7 is integrated on a substrate. A microarray for identification of Pseudomonas asplenii characterized in that the probe of claim 8 is integrated on a substrate. A microarray for identification of Pseudomonas avellanae characterized in that the probe of claim 9 is integrated on a substrate. 11. A microarray for identifying Pseudomonas cannabina , characterized in that the probe of claim 10 is integrated on a substrate. In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 96th base C of SEQ ID NO: 1, the 753th base G of SEQ ID NO: 1, the 754th nucleotide A of SEQ ID NO: 1, the 1162nd nucleotide A of SEQ ID NO: 1, Base 1177 of SEQ ID NO: 1, nucleotide 1177 of SEQ ID NO: 1, nucleotide 1178 of SEQ ID NO: 1, base 1313 of base 13 of SEQ ID NO: 1, base 1361 of base 1361 of SEQ ID NO: The 1369th base G of SEQ ID NO: 1, the 1371th to 1373th base GTA of SEQ ID NO: 1, the 1397th base G of SEQ ID NO: 1, the 1399th base A of SEQ ID NO: 1, the 1401th base of SEQ ID NO: C, the 1420th base T of SEQ ID NO: 1, the 1440th base T of SEQ ID NO: 1, the 1446th base A of SEQ ID NO: 1, the 1457th base A of SEQ ID NO: 1, the 1563th base C of SEQ ID NO: 1, Nucleotide C at position 1727, nucleotide 1727 at nucleotide number 1, nucleotides 1730 to 1731 of SEQ ID NO: 1, A nucleotide A at position 1732 of SEQ ID NO: 1, a nucleotide at position 2586 of SEQ ID NO: 1, a nucleotide A at position 2587 of SEQ ID NO: 1, a nucleotide G at position 2597 of SEQ ID NO: 1, a nucleotide A at nucleotide 2722 of SEQ ID NO: 1, And the 2740th base T of SEQ ID NO: 1. The marker for identification of Pseudomonas agaricus is characterized by the fact that the nucleotide sequence of SEQ ID NO: In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the nucleotide sequence of the nucleotide sequence of SEQ ID NO: 1, G at position 1159, nucleotide 1370 of SEQ ID NO: 1, nucleotide 1728 of SEQ ID NO: 1, nucleotide 2256 of SEQ ID NO: 2262th base G of SEQ ID NO: 1, 2267th base G of SEQ ID NO: 1, 2286th base T of SEQ ID NO: 1, 2342th base G of SEQ ID NO: 1, 2358th nucleotide of SEQ ID NO: A base deletion and a 2549th base C of SEQ. ID. NO. 1, and a marker for identifying Pseudomonas amygdali . In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 96th base C of SEQ ID NO: 1, the 135th nucleotide T of SEQ ID NO: 1, the 169th nucleotide A of SEQ ID NO: 1, the 741th nucleotide A of SEQ ID NO: 1, Base 1032 of base No. 1, base 1034 of base sequence 1 of SEQ ID NO: 1, base 1035 of base A of SEQ ID NO: 1, base 1170 of base 1 of base sequence 1, nucleotide 1173 of base 1173 of base sequence 1, base 1176 of base sequence 1 Base G of SEQ ID NO: 1, nucleotide 1178 of SEQ ID NO: 1, nucleotide 1275 of SEQ ID NO: 1, base 1309 of base 130 of SEQ ID NO: 1, base 1313 of base 13 of SEQ ID NO: 1, base 1360 of base 13 of SEQ ID NO: 1, 1, the 1373th base G of SEQ ID NO: 1, the 1440th base T of SEQ ID NO: 1, the 1446th base A of SEQ ID NO: 1, the 1457th base A of SEQ ID NO: 1, the 1469th base of SEQ ID NO: T, the 1725th to 1727th base CTT of SEQ ID NO: 1, the 1729th base C of SEQ ID NO: 1, the Nucleotide B of nucleotide number 1730 to 1731, nucleotide 1732 of SEQ ID NO: 1, nucleotide 2182 of SEQ ID NO: 1, nucleotide 2192 of nucleotide 1 of SEQ ID NO: 1, nucleotide 2238 of nucleotide A of SEQ ID NO: 1, nucleotide 2250 of SEQ ID NO: 2351th base G of SEQ ID NO: 1, 2361th base G of SEQ ID NO: 1, 2361th base C of SEQ ID NO: 1, 2362nd base T of SEQ ID NO: 1, SEQ ID NO: The 2445th base G of SEQ ID NO: 1, the 2447th base A of SEQ ID NO: 1, the 2542th base G of SEQ ID NO: 1, the 2543th base A of SEQ ID NO: 1, the 2545th base T of SEQ ID NO: 1 The 2549th base A of SEQ ID NO: 1, the 2552th base C of SEQ ID NO: 1, the 2586th base C of SEQ ID NO: 1, the 2587th base A of SEQ ID NO: 1, the 2597th base G of SEQ ID NO: 1, 2615th base A of SEQ ID NO: 1, 2618th base G of SEQ ID NO: 1, 2618th base C of SEQ ID NO: 1, and 2794th nucleotide of SEQ ID NO: Pseudomonas Aspergillus you play (Pseudomonas asplenii) identify markers for, it characterized in that the group is substituted by A. In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 96th base C of SEQ ID NO: 1, the 1159th base G of SEQ ID NO: 1, the 1359th base A of SEQ ID NO: 1, the 1725th base C of SEQ ID NO: 1, 1, base 1730 of SEQ ID NO: 1, nucleotide 2358 of SEQ ID NO: 1, base 2586 of base C of SEQ ID NO: 1, base 2587 of base A of SEQ ID NO: 1, base 2597 of base 1 of SEQ ID NO: 1 Base G of SEQ ID NO: 1, nucleotide 2722 of SEQ ID NO: 1, nucleotide 2739 of nucleotide G of SEQ ID NO: 1, and nucleotide T of nucleotide 2740 of SEQ ID NO: 1. The marker for identifying Pseudomonas avellanae . In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 96th base C of SEQ ID NO: 1, the 1162nd nucleotide A of SEQ ID NO: 1, the 1397th nucleotide G of SEQ ID NO: 1, the 1725th nucleotide C of SEQ ID NO: 1, The nucleotide at position 1730 of SEQ ID NO: 1, the nucleotide at position 2358 of SEQ ID NO: 1, the nucleotide C at position 2586 of SEQ ID NO: 1, the nucleotide A at position 2587 of SEQ ID NO: 1, Base G at position 2631 of SEQ ID NO: 1, nucleotide 2722 at nucleotide 2722 of SEQ ID NO: 1, nucleotide 2724 to base 2724 of nucleotide number 1, nucleotide CG38 at nucleotide 2738 to nucleotide 2740 of nucleotide number 1 and nucleotide G at nucleotide 2755 A marker for identifying Pseudomonas cannabina which is substituted.
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Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GenBank Accession No. LJQS01000198.1 (2015.10.20)*
NCBI Reference Sequence No. NZ_AKBQ01000006.1 (2013.04.28)*
NCBI Reference Sequence No. NZ_JYHC01000266.1 (2015.08.19)*
NCBI Reference Sequence No. NZ_LJPX01000603.1 (2015.10.21)*
Prokaryotes, Vol. 6, pp. 646-703 (2006.)*

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