KR101444217B1 - Method of identifying Xanthomonas hyacinthi - Google Patents

Abstract

The present invention relates to a method for identification of Xanthomonas hyacinthi, and more particularly to a method for identifying a base of 23S rRNA isolated from a sample to identify Santomonas hyacinthi. The method for identifying Xanthomonas hyacinthi of the present invention and the probe for identification of Xanthomonas hyacinthi can effectively identify Santomonas hyacinthi from Xanthomonas.

Description

{Method of identifying Xanthomonas hyacinthi}

The present invention relates to a method for identification of Xanthomonas hyacinthi, and more particularly to a method for identifying a base of 23S rRNA isolated from a sample to identify Santomonas hyacinthi.

The genus Santomonas (Xanthomonas) is a bacteria involved in the denitrification of soil nitrogen. These include pathogenic bacteria such as peach tree bacterial pericarditis, non-black rot, citrus peptic ulcer disease, rice blight white leaf blight,

Xanthomonas hyacinthi is a proteobacteria species. These plant pathogenic bacteria cause yellow fever to hyacinth.

Dot-blot, PCR (PCR), etc. are used for gene identification of plant pathogenic bacteria. In particular, PCR is a widely used method because it is economical in terms of time, effort, and manpower than the dot-blot method. PCR (polymerase chain reaction) is a method for identifying a DNA fragment (primer) consisting of 10-20 bp in the genomic DNA of a bacterium and then using heat-resistant DNA polymerase The PCR amplification product is electrophoresed on an agarose gel or an acrylamide gel and ethidium bromide is added to the reaction mixture, bromide, and silver stain. The DNA polymorphism of the bacterium, such as the presence or absence of DNA bands, is identified and identified. Currently, the identification method of bacteria by PCR is mainly used for identification of bacterial diseases in humans and animals. However, in recent years, PCR identification method has been developed to identify plant pathogens and is used for identification of diseases and quarantine of agricultural products . In addition, the PCR identification method is shorter in time than other methods, is low in cost for identification, and is also economical because many samples can be assayed at the same time. Recently, PCR identification method that amplifies nucleic acid which is a genetic material among the identification methods of Santomonas (Xanthomonas) is most preferred.

However, existing PCR identification methods are difficult to classify due to genetic similarity, and a solution to overcome this problem is needed.

Among the existing microorganisms of the genus Santomonas, Xanthomonas oryzae pv. Oryzae, Xanthomonas campestris pv. Vesicatoria, Xanthomonas campestris pv. Campestris, Xanthomonas axonopodis pv. citr and Xanthomonas axonopodis pv. glycines. However, it has been reported that a method for identifying Santomonas hyacinthi (Xanthomonas hyacinthi) from the genus Santomonas (Xanthomonas) Have not yet been studied. It is required to develop a method for identifying Santomonas hyacinthi and its hospital type through a species or a hospital type specific base and a base combination among plant pathogenic bacteria.

Accordingly, the inventors of the present invention discovered the difference in the 23S rRNA sequence of Santomonas bacteria during the study to identify Santomonas hyacinthi (Xanthomonas hyacinthi) more accurately and rapidly from the genus Santomonas (Xanthomonas) Identification method was developed.

Accordingly, an object of the present invention is to provide a method for detecting (i) isolating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 78th base G of SEQ ID NO: 1, the 85th base C of SEQ ID NO: 1, the 86th base C of SEQ ID NO: 1, the 97th base A , Nucleotide No. 682 of SEQ ID NO: 1, nucleotide 692 of SEQ ID NO: 1, nucleotide 698 of SEQ ID NO: 1, nucleotide C at position 750 of SEQ ID NO: 1, nucleotide C at position 750 of SEQ ID NO: 1, nucleotide 751 of SEQ ID NO: 764th base G of SEQ ID NO: 1, 1306th base G of SEQ ID NO: 1, 1310th base A of SEQ ID NO: 1, 1359th base T of SEQ ID NO: 1, 1361th base T of SEQ ID NO: 1, Base 1367 of SEQ ID NO: 1, nucleotide 1486 of SEQ ID NO: 1, nucleotide 1550 of nucleotide G of SEQ ID NO: 1, base 1557 of nucleotide C of SEQ ID NO: 1, nucleotide C of nucleotide 1566 of SEQ ID NO: 1, nucleotide 1611 of SEQ ID NO: 1 The base T at position 1668 of SEQ ID NO: 1, the nucleotide C at position 1720 of SEQ ID NO: 1, the nucleotide G at position 1721 of SEQ ID NO: 1, the base 172 of SEQ ID NO: 1 A nucleotide sequence of nucleotides 1727 to 1730 of SEQ ID NO: 1, nucleotides 1730 to 1730 of SEQ ID NO: 1, nucleotide G of nucleotide 1731 of SEQ ID NO: 1, nucleotide C of nucleotide 1732 of SEQ ID NO: Nucleotide G at position 1733 of SEQ ID NO: 1, nucleotide G at position 1858 of SEQ ID NO: 1, nucleotide C at position 2050 of SEQ ID NO: 1, nucleotide 2136 of nucleotide 2136 of SEQ ID NO: 1, nucleotides 2146 The base 2147 of SEQ ID NO: 1, the nucleotide 2149 of SEQ ID NO: 1, the nucleotide 2158 of SEQ ID NO: 1, the nucleotide 2160 of SEQ ID NO: 1, the nucleotide 2161 of SEQ ID NO: 1, The 2181th base C of SEQ ID NO: 1, the 2183th base G of SEQ ID NO: 1, the 2190th base C of SEQ ID NO: 1, the 2191st base T of SEQ ID NO: 1, the 2192th base T of SEQ ID NO: Base G, nucleotide G at position 2206 of SEQ ID NO: 1, nucleotide G at position 2495 of SEQ ID NO: 1, nucleotide 2451 of SEQ ID NO: 1, sequence A 2582th base G of SEQ ID NO: 1, 2603th base G of SEQ ID NO: 1, 2605th base A of SEQ ID NO: 1, 2610th base A of SEQ ID NO: 1, 2625th base of SEQ ID NO: 1 Base A at position 2626, nucleotide 2626 at position 1 in SEQ ID NO: 1, nucleotide 2715 at nucleotide 1 in SEQ ID NO: 1, nucleotide 2726 at nucleotide 2726 in SEQ ID NO: 1, nucleotide 2737 in nucleotide 1 of SEQ ID NO: 1, (Xanthomonas hyacinthi) including a step of judging the plant to be a plant of the genus Xanthomonas hyacinthi.

Another object of the present invention is a polynucleotide comprising 20-100 consecutive DNA sequences comprising the 78th base G of SEQ ID NO: 1, 20-100 consecutive DNAs comprising the 85th base C of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 86th base C of SEQ ID NO: 1, 20-100 consecutive DNAs comprising the 97st nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 682nd nucleotide C of SEQ ID NO: 1, 20-100 consecutive DNAs comprising the 692nd nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 698th base C of SEQ ID NO: 1, a polynucleotide consisting of the 750th base C of SEQ ID NO: 1 Comprises a polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 751st base A of SEQ ID NO: 1, 764th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences comprising the 765th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 765th nucleotide G of SEQ ID NO: 1, 1306th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences comprising the 1310th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1310th base A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1367th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1486th nucleotide T of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1550th base G of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising the 1557th base C of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1566th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 1611th base T of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-20 consecutive DNA sequences comprising the 1720th base C of SEQ ID NO: A polynucleotide consisting of 100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1721 nucleotides G of SEQ ID NO: 1, a polynucleotide consisting of 20- A polynucleotide consisting of 100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1727th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1727th base A of SEQ ID NO: A polynucleotide consisting of 100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1730th base C of SEQ ID NO: 1, a polynucleotide consisting of 2031 consecutive DNA sequences consisting of 1731th base G of SEQ ID NO: A polynucleotide consisting of 100 consecutive DNA sequences, 20-100 consecutive DNA sequences comprising 1732 base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1733th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 1735th nucleotide T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1858th base G of SEQ ID NO: 1, 2050 consecutive DNA sequences comprising 2050th nucleotide C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2136th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2146th nucleotide T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2147th base A of SEQ ID NO: 1, 20-100 nucleotides comprising 2149th nucleotide C of SEQ ID NO: 1 , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2158th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2160th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2161th nucleotide A of SEQ ID NO: 1, 20-100 A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2183th nucleotide A of SEQ ID NO: A polynucleotide consisting of 20-100 contiguous DNA sequences comprising the 2190th base C of SEQ ID NO: 1, , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2191th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2192th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2206th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2495th nucleotide G of SEQ ID NO: 1 , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2451th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2582th base C of SEQ ID NO: 1 , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2591 base G of SEQ ID NO: 1, 20-100 nucleotides comprising the 2603 base G of SEQ ID NO: 1 A polynucleotide consisting of a consecutive DNA sequence, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2605th base A of SEQ ID NO: 1, 20-100 of a polynucleotide consisting of 2610th base A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2625th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2626th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2715th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2726th base T of SEQ ID NO: 1, a polynucleotide consisting of a consecutive DNA sequence, (Xanthomonas hyacinthi) comprising at least one polynucleotide selected from the group consisting of polynucleotides consisting of 20-100 consecutive DNA sequences containing the 2737th base G of SEQ ID NO: 1 and complementary polynucleotides thereof. And a probe for use with the probe.

In order to achieve the above object, the present invention provides a method for identification of Santomonas hyacinthi.

In order to accomplish another object of the present invention, there is provided a probe for identification of Santomonas hyacinthi.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for identification of Santomonas hyacinthi (Xanthomonas hyacinthi).

That is, the present invention identifies Santomonas hyacinthi (Xanthomonas hyacinthi) by securing the base combination of the 23S rRNA gene of the genus Xanthomonas.

More specifically, the method for identifying Xanthomonas hyacinthi of the present invention comprises the steps of: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is the 78th base G of SEQ ID NO: 1, the 85th base C of SEQ ID NO: 1, the 86th base C of SEQ ID NO: 1, the 97th base A , Nucleotide No. 682 of SEQ ID NO: 1, nucleotide 692 of SEQ ID NO: 1, nucleotide 698 of SEQ ID NO: 1, nucleotide C at position 750 of SEQ ID NO: 1, nucleotide C at position 750 of SEQ ID NO: 1, nucleotide 751 of SEQ ID NO: 764th base G of SEQ ID NO: 1, 1306th base G of SEQ ID NO: 1, 1310th base A of SEQ ID NO: 1, 1359th base T of SEQ ID NO: 1, 1361th base T of SEQ ID NO: 1, Base 1367 of SEQ ID NO: 1, nucleotide 1486 of SEQ ID NO: 1, nucleotide 1550 of nucleotide G of SEQ ID NO: 1, base 1557 of nucleotide C of SEQ ID NO: 1, nucleotide C of nucleotide 1566 of SEQ ID NO: 1, nucleotide 1611 of SEQ ID NO: 1 The base T at position 1668 of SEQ ID NO: 1, the nucleotide C at position 1720 of SEQ ID NO: 1, the nucleotide G at position 1721 of SEQ ID NO: 1, the base 172 of SEQ ID NO: 1 A nucleotide sequence of nucleotides 1727 to 1730 of SEQ ID NO: 1, nucleotides 1730 to 1730 of SEQ ID NO: 1, nucleotide G of nucleotide 1731 of SEQ ID NO: 1, nucleotide C of nucleotide 1732 of SEQ ID NO: Nucleotide G at position 1733 of SEQ ID NO: 1, nucleotide G at position 1858 of SEQ ID NO: 1, nucleotide C at position 2050 of SEQ ID NO: 1, nucleotide 2136 of nucleotide 2136 of SEQ ID NO: 1, nucleotides 2146 The base 2147 of SEQ ID NO: 1, the nucleotide 2149 of SEQ ID NO: 1, the nucleotide 2158 of SEQ ID NO: 1, the nucleotide 2160 of SEQ ID NO: 1, the nucleotide 2161 of SEQ ID NO: 1, The 2181th base C of SEQ ID NO: 1, the 2183th base G of SEQ ID NO: 1, the 2190th base C of SEQ ID NO: 1, the 2191st base T of SEQ ID NO: 1, the 2192th base T of SEQ ID NO: Base G, nucleotide G at position 2206 of SEQ ID NO: 1, nucleotide G at position 2495 of SEQ ID NO: 1, nucleotide 2451 of SEQ ID NO: 1, sequence A 2582th base G of SEQ ID NO: 1, 2603th base G of SEQ ID NO: 1, 2605th base A of SEQ ID NO: 1, 2610th base A of SEQ ID NO: 1, 2625th base of SEQ ID NO: 1 Base A at position 2626, nucleotide 2626 at position 1 in SEQ ID NO: 1, nucleotide 2715 at nucleotide 1 in SEQ ID NO: 1, nucleotide 2726 at nucleotide 2726 in SEQ ID NO: 1, nucleotide 2737 in nucleotide 1 of SEQ ID NO: 1, And a step of judging to be Monas hyacinth (Xanthomonas hyacinthi).

The present invention also provides a probe for identifying Xanthomonas hyacinthi comprising a combination of gene bases.

That is, the present invention identifies Santomonas hyacinthi (Xanthomonas hyacinthi) by securing the base combination of the 23S rRNA gene of the genus Xanthomonas.

More specifically, the probe for identifying Santomonas hyacinthi of the present invention comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 78th base G of SEQ ID NO: 1, a polynucleotide consisting of the 85th base C A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 86th base C of SEQ ID NO: 1, a polynucleotide consisting of 97th base A A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 682st nucleotide C of SEQ ID NO: 1, , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, and a 20-100 consecutive DNA sequences comprising 698th nucleotide C of SEQ ID NO: 1 Comprises a polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 750th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 751st nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 764th base T of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising 765th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1306th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 1310th nucleotide A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1359th base T of SEQ ID NO: 1, a polynucleotide consisting of the 1361th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1367th nucleotide A of SEQ ID NO: 1, a polynucleotide consisting of 1486 nucleotides T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1550th nucleotide G of SEQ ID NO: 1, a polynucleotide consisting of 1557th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1566th nucleotide C of SEQ ID NO: 1, a 1611th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1668th nucleotide G of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1720th base C of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising 1721th base G of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 1726 of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 1727th nucleotide T of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1729th base A of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising 1730th base C of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1731 base G of SEQ ID NO: 1, a polynucleotide consisting of 1732 base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences comprising 1733th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1733th nucleotide G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences comprising the 1858th nucleotide G of SEQ ID NO: 1, 2050th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 consecutive DNA sequences comprising the 2136th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2136th nucleotide C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences consisting of 20-100 contiguous DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2149th base C of SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising the 2158th base G of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2160th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2161th base A of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2181 base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2182 base A of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2183th base G of SEQ ID NO: 1, 2190th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2191th base T of SEQ ID NO: 1, 2192th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2206th base G of SEQ ID NO: 1, a polynucleotide consisting of 2495th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2451th base A of SEQ ID NO: 1, 2582th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2591th base G of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2603th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2605th base A of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2610th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2625th base A of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2626th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2649th nucleotide T of SEQ ID NO: 1 Polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2715th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2726th base T of SEQ ID NO: A polynucleotide consisting of 100 consecutive DNA sequences, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2737th base G of SEQ ID NO: 1, and complementary polynucleotides thereof. And a polynucleotide.

In the present invention, the nucleic acid may be separated in step (a) according to a method commonly used in the art, and may be carried out using a commercially available extraction kit.

Also, the base sequence in step (b) may be performed according to automatic or manual base sequence analysis methods known in the art. Preferably, PCR is performed on a test specimen or a sample to perform PCR corresponding to 23S rRNA And the sequence of the product can be analyzed and analyzed according to a known nucleotide sequence analysis method.

In the step (c), the nucleotide sequence corresponding to the judgment point in the microorganism of the present invention can be identified based on SEQ ID NO: 1 to determine whether the microorganism corresponds to the present invention.

Meanwhile, the present invention provides a microarray for detecting microorganisms of the present invention, wherein the probe of the present invention is integrated on a substrate. The microarray consists of a conventional microarray except that it contains a polynucleotide of the present invention, and a method for producing a microarray by immobilizing a polynucleotide used as a marker on an organ is well known in the art.

Further, the present invention is a marker for detecting a microorganism according to the present invention, wherein a nucleotide having a nucleotide sequence shown in SEQ ID NO: 1 is substituted for each of the bases corresponding to the judgment point of the microorganism of the present invention. Lt; / RTI > The above judgment points are as shown in Table 3.

The nucleotide, probe or marker of the present invention may be DNA or RNA, and it is obvious to those skilled in the art that the thymine described in the sequence is replaced by uracil in the case of RNA.

Therefore, when the identification method of Xanthomonas hyacinthi of the present invention and the probe for identification of Xanthomonas hyacinthi are used, it is possible to effectively identify Santomonas hyacinthi (Xanthomonas hyacinthi) from the genus Santomonas (Xanthomonas) .

Fig. 1 shows the result of comparative analysis of nucleotide sequences of Santomonas campestris pv. Campestris and Xanthomonas hyacinthi.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Example  1>

X. hyacinthi  And Hospitals  23S rRNA  analysis

Through the following experimental procedure, a probe for identification of bacteria belonging to the genus Xanthomonas was designed. The total RNA isolation, base sequence amplification and analysis methods used in the present invention are as follows.

&Lt; 1-1 > rRNA  Amplification

After the DNA of X. hyacinthi was isolated, PCR was performed using primer pairs as shown in Table 1 below, which can isolate 23s rRNA of bacteria of the genus Xanthomonas.

Name of the primer Primer sequence Xan23SF5 (omnidirectional) 5'-TGGTCAAGCCGCACGGATCATTAGTAT-3 ' Xan23SR6 (reverse direction) 5'-ACGTGGATAGCCTGCGAAAAGTGTC-3 ' Xan23SF7 (Omnidirectional) 5'-GAGACCGCCCCAGTCAAACTAC-3 ' Xan23SR7 (reverse direction) 5'-ACCTTTTGTATAATGGGTCAACG-3 ' Xan23SF9 (Omnidirectional) 5'-GTCAAGCCGCACGGATCATTAGTAT-3 ' Xan23SR9 (reverse direction) 5'-GTCGGGGAGCTGGCAACAAG-3 '

The PCR reaction consisted of 20 ng of isolated total DNA, 10 pmoles of downstream primer, 10 pmole of upstream primer, 0.2 U of Taq DNA polymerase, 10 times Polymerase chain reaction buffer (100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl ₂ , pH 8.3) was added and distilled water was added to make 50 μl of reaction solution.

The reaction solution was heated at 95 ° C. for 5 minutes and then amplified 30 times at 95 ° C. for 30 seconds, 65 ° C. for 60 seconds, and 72 ° C. for 180 seconds. Finally, the reaction solution was treated at 72 ° C. for 10 minutes with a PCR machine (DNA Engine PTC-200) Respectively. After amplification, the PCR product was electrophoresed using 1 × TBE buffer and 1.5% agarose gel, stained with ethidium bromide, and then confirmed by ultraviolet lamp.

<1-2> Amplified 23s rRNA  Sequencing

The nucleotide sequences of the PCR products obtained in Example <1-1> were analyzed using BioEdit Sequence Alignment Editor.

After analysis, X. campestris pv. We compared the 23s rRNA sequences of campestris. X. campestris pv. For the campestris 23s rRNA sequence, the information (NC_003902.1) published in Genbank was used. The 23s rRNA sequences of the bacteria used in this experiment are summarized in the sequence numbers as shown in Table 2 below.

SEQ ID NO: Germ One X. campestris pv. campestris 2 X. hyacinthi

< Example  2>

Interspecific  23s rRNA  Sequence comparative analysis

The nucleotide sequence differences between SEQ ID NOS: 1 and 2 in Table 2 were compared. The nucleotide sequences were compared using the BioEdit Sequence Alignment Editor.

The results of the analysis are shown in Fig. 1, and X. campestris pv. Table 3 summarizes the positions of base sequence differences with X. hyacinthi based on the nucleotide sequence of Campestris (SEQ ID NO: 1).

Position in sequence number Changed base No. 78 of SEQ ID NO: 1 G No. 85 in SEQ ID NO: 1 C The 86 th C No. 97 in SEQ ID NO: 1 A No. 682 of SEQ ID NO: 1 C No. 692 of SEQ ID NO: 1 T No. 698 of SEQ ID NO: 1 C No. 750 in SEQ ID NO: 1 C No. 751 of SEQ ID NO: A No. 764 of SEQ ID NO: 1 T No. 765 of SEQ ID NO: 1 G 1306 of SEQ ID NO: 1 G No. 1310 of SEQ ID NO: A No. 1359 of SEQ ID NO: T No. 1361 of SEQ ID NO: T The 1367th A No. 1486 of SEQ ID NO: T No. 1550 of SEQ ID NO: G 1557 &lt; th &gt; C No. 1566 of SEQ ID NO: C 1611 of SEQ ID NO: 1 T No. 1668 of SEQ ID NO: G 1720 &lt; th &gt; C No. 1721 of SEQ ID NO: 1 G No. 1726 of SEQ ID NO: T The 1727nd of SEQ ID NO: T The 1729nd of SEQ ID NO: A No. 1730 of SEQ ID NO: C No. 1731 of SEQ ID NO: G No. 1732 of SEQ ID NO: C No. 1733 of SEQ ID NO: G No. 1735 of SEQ ID NO: T 1858 of SEQ ID NO: 1 G The 2050th C The 21st place in SEQ ID NO: C The 2146th T 2147 &lt; th &gt; A 2149 &lt; th &gt; C No. 2158 of SEQ ID NO: G No. 2160 of SEQ ID NO: G No. 2161 of SEQ ID NO: A No. 2181 of SEQ ID NO: C No. 2182 of SEQ ID NO: A 2183 of SEQ ID NO: G 2190 &lt; th &gt; C No. 2191 of SEQ ID NO: T 2192 &lt; th &gt; G No. 2206 of SEQ ID NO: G The 2495th G No. 2541 of SEQ ID NO: A No. 2582 of SEQ ID NO: C No. 2591 of SEQ ID NO: 1 G No. 2603 of SEQ ID NO: G No. 2605 of SEQ ID NO: A No. 2610 of SEQ ID NO: 1 A No. 2625 of SEQ ID NO: 1 A 2626nd of SEQ ID NO: G The 2649th T 2715 th in SEQ ID NO: 1 C 2726nd of SEQ ID NO: T 2737 th in SEQ ID NO: G

Therefore, the bacterium can be identified using the difference of the bases shown in [Table 3] above.

As described above, when the identification method of Xanthomonas hyacinthi of the present invention and the probe for identification of Xanthomonas hyacinthi are used, it is possible to effectively identify Santomonas hyacinthi (Santomonas hyacinthi) from the genus Santomonas (Xanthomonas) The possibility of industrial use is high.

<110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Method of identifying Xanthomonas hyacinthi <130> NP12-0059 <160> 8 <170> Kopatentin 2.0 <210> 1 <211> 2883 <212> DNA <213> X. campestris pv. campestris NC_003902 <400> 1 atggtcaagc cgcacggatc attagtatca gttagctcaa tacattgctg tacttacaca 60 cctgacctat caaccacata gtctatatgg ttcctttagg gggcttgtgc cccgggaagt 120 ctcatcttga ggcgcgcttc ccgcttagat gctttcagcg gttatcgctt ccgaacatag 180 ctacccggca atgccactgg cgtgacaacc ggaacaccag aggttcgtcc actccggtcc 240 tctcgtacta ggagcagccc ctctcaaact tccaacgccc atggcagata gggaccgaac 300 tgtctcacga cgttctgaac ccagctcgcg taccacttta aatggcgaac agccataccc 360 ttgggaccga ctacagcccc aggatgtgat gagccgacat cgaggtgcca aacaccgccg 420 tcgatatgaa ctcttgggcg gtatcagcct gttatccccg gagtaccttt tatccgttga 480 gcgatggccc ttccatacag aaccaccgga tcactaagac ctactttcgt acctgcttga 540 tccgtcgatc ttgcagtcaa gcacgcttat gcctttgcac acagtgcgcg atgtccgacc 600 gcgctgagcg taccttcgtg ctcctccgtt actctttagg aggagaccgc cccagtcaaa 660 ctacccacca tacacggtcc ctgatccgga taacggatct aggttagaac gtcaagcacg 720 acagggtggt atttcaaggt tggctccact gcagctagcg ccacagtttc atagcctccc 780 acctatccta cacagacgaa ctcaacgttc agtgtaaagc tatagtaaag gttcacgggg 840 tctttccgtc ttgccacggg aacgctgcat cttcacagcg atttcaattt cactgagtct 900 cgggtggaga cagcgccgct gtcgttacgc cattcgtgca ggtcggaact tacccgacaa 960 ggaatttcgc taccttagga ccgttatagt tacggccgcc gtttactggg gcttcgatca 1020 agagcttcgc cttgcggctg accccatcaa ttaaccttcc agcaccgggc aggcgtcaca 1080 ccctatacgt ccactttcgt gtttgcagag tgctgtgttt ttgataaaca gtcgcagcgg 1140 cctggtttct gcgaccctct tcagctatag ctcgcatgag ccaccaaaaa gggtgcacct 1200 tctcccgaag ttacggtgcc atgttgccta gttccttcac ccgagttctc tcaagcgcct 1260 gagaattctc atcctaccca cctgtgtcgg tttacggtac ggtcttcgtg agctgaagct 1320 taggagcttt tcctggaagc gtggtatcag tgacttcgcc ataaaggctc gtctcggtgc 1380 tcggtcttaa aggatcccgg atttgccaaa gatccaaacc taccgccttt ccccgggaca 1440 accaacgccc ggtacaccta accttctccg tccctccatc gcactcacgc gaggtgcagg 1500 aatattaacc tgcttcccat cgactacggc tttcgccctc gccttaggga ccgactaacc 1560 ctgcgtcgat taacgttgcg caaggaaacc ttgggctttc ggcgtgcggg cttttcaccc 1620 gcattatcgt tactcatgtc agcattcgca cttccgatac ctccagcaga cttctcaatc 1680 caccttcgca ggcttacgga acgctcctct accgcgcata aaaccgaagt tttacgcacc 1740 ccaagcttcg gttcactgct tagccccgtt aaatcttccg cgcagaccga ctcgaccagt 1800 gagctattac gctttcttta aagggtggct gcttctaagc caacctcctg gctgtctatg 1860 cctttccaca tcgttttcca cttagcagtg aatttgggac cttagctgtg ggtctgggtt 1920 gtttcccttt tcacgacgga cgttagcacc cgccgtgtgt ctcccggata gtacgtactg 1980 gtattcggag tttgcaatgg tttggtaagt cgcgatgacc ccctagccat aacagtgctc 2040 tacccccagt agtattcgtc cgaggcgcta cctaaatagc tttcgaggag aaccagctat 2100 ctccgggttc gattagcttt tcactcctaa tcacagctca tccccgtctt ttgcaacaga 2160 cgtgggttcg ggcctccagt acctgttacg gcaccttcac cctggccatg actagatcac 2220 ccggtttcgg gtctactgcc cgcgactatg cgcccttatc agactcggtt tcccttcgcc 2280 tcccctatac ggttaagctt gccacgaaca gtaagtcgct gacccattat acaaaaggta 2340 cgcagtcact cttgcgagct cctactgctt gtacgcacac ggtttcagga tctatttcac 2400 tcccctctcc ggggttcttt tcgcctttcc ctcacggtac tggttcacta tcggtcggtc 2460 aggagtattt agccttggag gatggtcccc ccatattcag acagggtttc acgtgccccg 2520 ccctactcgt cttcactgga gtggcccttt taaatacagg gctatcacct tctatggcca 2580 atctttccag attgtttttc taaagccatt ccagcttaag ggctgttccc cgttcgctcg 2640 tcactactca gggaatctcg gttgatttct tttcctccgg ttacttagat atttcagttc 2700 accgggttcg cttcaagcag ctatgaattc actgcaagat actgccgaag cagtgggttt 2760 ccccattcgg atattgccgg atcaaagctt gttgccagct ccccgacact tttcgcaggc 2820 taccacgtcc ttcatcgcct ctgaccgcct aggcatccac cgtgtgcgct tattcgcttg 2880 acc 2883 <210> 2 <211> 2713 <212> DNA <213> X hyacinthi BC2578 <400> 2 acacacctga cctatcaacc acgtagtctc catggttcct taagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccccgat ccggattacg gacctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggttggct ccaccacagc tagcgccatg gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct gcgtaagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact tcgtctaaaa gactcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact tacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt aggggccgac 1500 tcaccctgcg ccgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggttttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcatcgctta acgcgatgca 1680 ccccaagctt cggttcactg cttagccccg ttaaatcttc cgcgcagacc gactcgacca 1740 gtgagctatt acgctttctt taaagggtgg ctgcttctaa gccaacctcc tggctgtctg 1800 tgcctttcca catcgttttc cacttagcag tgaatttggg accttagctg tgggtctggg 1860 ttgtttccct tttcacgacg gacgttagca cccgccgtgt gtctcccgga tagtacgtac 1920 tggtattcgg agtttgcaat ggtttggtaa gtcgcgatga ccccctagcc ataacagtgc 1980 tctaccccca gcagtattcg tccgaggcgc tacctaaata gctttcgagg agaaccagct 2040 atctccgggt tcgattagct tttcactcct aatcacacct catcccctac ctttgcaacg 2100 gt; acccggtttc gggtctactg cccgcgacta tgcgccctta tcagactcgg tttcccttcg 2220 cctcccctat acggttaagc ttgccacgaa cagtaagtcg ctgacccatt atacaaaagg 2280 tacgcagtca ctcttgcgag ctcctactgc ttgtacgcac acggtttcag gatctatttc 2340 actcccctct ccggggttct tttcgccttt ccctcacggt actggttcac tatcggtcgg 2400 tcaggagtat ttagccttgg aggatggtcc ccccatgttc agacagggtt tcacgtgccc 2460 cgccctactc gtcttcactg gaatggccct tttaaataca gggctatcac cttctatggc 2520 caacctttcc aggttgtttt tctagaacca taccagctta agggctagtc cccgttcgct 2580 cgtcactact tagggaatct cggttgattt cttttcctcc ggttacttag atatttcagt 2640 tcaccgggtt cgcttccagc agctatgtat tcactgcagg atactgccga agcagtgggt 2700 ttccccattc gga 2713 <210> 3 <211> 27 <212> DNA <213> Xan23SF5 <400> 3 tggtcaagcc gcacggatca ttagtat 27 <210> 4 <211> 25 <212> DNA <213> Xan23SR6 <400> 4 acgtggatag cctgcgaaaa gtgtc 25 <210> 5 <211> 22 <212> DNA <213> Xan23SF7 <400> 5 gagaccgccc cagtcaaact ac 22 <210> 6 <211> 23 <212> DNA <213> Xan23SR7 <400> 6 accttttgta taatgggtca acg 23 <210> 7 <211> 25 <212> DNA <213> Xan23SF9 <400> 7 gtcaagccgc acggatcatt agtat 25 <210> 8 <211> 20 <212> DNA <213> Xan23SR9 <400> 8 gtcggggagc tggcaacaag 20

Claims (4)

(a) separating 23S rRNA from the sample;
(b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And
(c) the nucleotide sequence identified in step (b) is the 78th base G of SEQ ID NO: 1, the 85th base C of SEQ ID NO: 1, the 86th base C of SEQ ID NO: 1, the 97th base A of SEQ ID NO: The nucleotide at position 682 of SEQ ID NO: 1, the nucleotide at position 692 of SEQ ID NO: 1, the nucleotide at position 698 of SEQ ID NO: 1, the nucleotide C at position 750 of SEQ ID NO: 1, the nucleotide 751 of SEQ ID NO: 1, Base T at position 1, base G at position 765 of SEQ ID NO: 1, base 1306 of base G of SEQ ID NO: 1, nucleotide 1310 of base 13 of SEQ ID NO: 1, base 1359 of base 135 of SEQ ID NO: 1, nucleotide 1361 of SEQ ID NO: Base 1367 of base No. 1, base 1486 of base 1 of SEQ ID NO: 1, base 1550 of base G of base 1, base 1557 of base 1 of base 1, base 1566 of base 1 of base 1, base 1611 of base 1 Base T at position 1668 of SEQ ID NO: 1, nucleotide C at position 1720 of SEQ ID NO: 1, nucleotide G at nucleotide 1721 of SEQ ID NO: 1, nucleotide 1726 of SEQ ID NO: 1 The 1727th base T of SEQ ID NO: 1, the 1729th base A of SEQ ID NO: 1, the 1730th base C of SEQ ID NO: 1, the 1731th base G of SEQ ID NO: 1, the 1732th base C of SEQ ID NO: 1, Nucleotide G at position 1733 of SEQ ID NO: 1, nucleotide G at position 1858 of SEQ ID NO: 1, nucleotide C at position 2050 of SEQ ID NO: 1, nucleotide 2136 of nucleotide 2136 of SEQ ID NO: 1, nucleotide 2146 of SEQ ID NO: 2147 base C of SEQ ID NO: 1, 2158th base G of SEQ ID NO: 1, 2160th base G of SEQ ID NO: 1, 2161th base A of SEQ ID NO: 1, SEQ ID NO: 2181 base B of SEQ ID NO: 1, 2181 base B of SEQ ID NO: 1, 2183 base G of SEQ ID NO: 1, 2190 base C of SEQ ID NO: 1, 2191 base T of SEQ ID NO: 1, G, 2206th base G of SEQ ID NO: 1, 2495th base G of SEQ ID NO: 1, 2451th base A of SEQ ID NO: 1, 2582th base G of SEQ ID NO: 1, 2603th base G of SEQ ID NO: 1, 2605th base A of SEQ ID NO: 1, 2610th base A of SEQ ID NO: 1, 2625th base A of SEQ ID NO: A, 2626th base G of SEQ ID NO: 1, 2649th base T of SEQ ID NO: 1, 2715th base C of SEQ ID NO: 1, 2726th base T of SEQ ID NO: 1, and 2737th base G of SEQ ID NO: (Xanthomonas hyacinthi) comprising the step of judging as hyacinth (Xanthomonas hyacinthi).
A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 78th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive sequences comprising the 85th base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 86th base C of SEQ ID NO: 1, 20-100 consecutive sequences comprising the 97th base A of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 682nd nucleotide C of SEQ ID NO: 1, 20-100 consecutive nucleotides comprising the 692nd nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 698th base C of SEQ ID NO: 1, a polynucleotide consisting of a polynucleotide consisting of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 750th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 751st nucleotide A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences containing the 764th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 765th nucleotide G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1306th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1310th base A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1359th base T of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1361th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1367th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1486th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1550th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1557th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1566th base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1611th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th base G of SEQ ID NO: 1, 1720 of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the base C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1721 nucleotides G of SEQ ID NO: 1, 1726 of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the base T, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1727th nucleotide T of SEQ ID NO: 1, 1729 of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the base A, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1730th base C of SEQ ID NO: 1, a polynucleotide consisting of 1731 A polynucleotide consisting of 20-100 consecutive DNA sequences containing the base G, 20-100 consecutive DNA sequences comprising the 1732th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1733th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 1735th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1858th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2050th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the polynucleotide consisting of 2136 base C of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2146th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2147th base A of SEQ ID NO: 1, a polynucleotide consisting of the 2149th nucleotide of SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2158th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide C, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2161 base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2182 base A of SEQ ID NO: 1, a polynucleotide consisting of 2083 consecutive DNA sequences comprising SEQ ID NO: A polynucleotide consisting of 20-100 consecutive DNA sequences comprising base G, 20-100 consecutive DNA sequences comprising 2190th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2191 base T of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2192th base G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2206th base G of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 2495th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2451th base A of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2582th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2591th base G of SEQ ID NO: 1, a polynucleotide consisting of the 2603th salt of SEQ ID NO: 1 G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2605th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2605th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: A, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2625th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2625th base A of SEQ ID NO: 1, G, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th nucleotide T of SEQ ID NO: 1, C, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising 2726th nucleotides T of SEQ ID NO: 1 Is a polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences containing the 2737th base G of SEQ ID NO: 1, and a probe composition for identification of Xanthomonas hyacinthi comprising both complementary polynucleotides thereof .
A microarray for the detection of Xanthomonas hyacinthi characterized in that the probe composition of claim 2 is integrated on a substrate.
In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 78th base G of SEQ ID NO: 1, the 85th nucleotide C of SEQ ID NO: 1, the 86th nucleotide C of SEQ ID NO: 1, the 97th nucleotide A of SEQ ID NO: 1, Base No. 692 of SEQ ID NO: 1, nucleotide No. 692 of SEQ ID NO: 1, base C at position 698 of SEQ ID NO: 1, base C at position 750 of SEQ ID NO: 1, nucleotide 751 of base No. 751 of SEQ ID NO: 1, nucleotide No. 764 Base T of SEQ ID NO: 1, nucleotide G at position 765 of SEQ ID NO: 1, nucleotide G at position 1306 of SEQ ID NO: 1, nucleotide A at position 1310 of SEQ ID NO: 1, nucleotide T at position 1359 of SEQ ID NO: 1, nucleotide 1361 of nucleotide No. 1361 of SEQ ID NO: 1, base 1367 of SEQ ID NO: 1, nucleotide T at position 1486 of SEQ ID NO: 1, base 1550 of base B of SEQ ID NO: 1, nucleotide C at position 1557 of SEQ ID NO: 1, base 1566 of base C of SEQ ID NO: 1, base 1611 of base 16 T, the 1668th base G of SEQ ID NO: 1, the 1720th base C of SEQ ID NO: 1, the 1721st base of SEQ ID NO: 1 G, nucleotide 1726 of SEQ ID NO: 1, nucleotide 1727 of SEQ ID NO: 1, nucleotide 1729 of nucleotide 1 of SEQ ID NO: 1, nucleotide 1730 of nucleotide 1730 of SEQ ID NO: 1, nucleotide 1731 of nucleotide 1731 of SEQ ID NO: 1, Nucleotide C at position 1733 of SEQ ID NO: 1, nucleotide T at position 1735 of SEQ ID NO: 1, nucleotide G at nucleotide 1858 of SEQ ID NO: 1, nucleotide C at position 2050 of SEQ ID NO: 1, nucleotide C The 2146th base T of SEQ ID NO: 1, the 2147th base A of SEQ ID NO: 1, the 2149th base C of SEQ ID NO: 1, the 2158th base G of SEQ ID NO: 1, the 2160th base G of SEQ ID NO: 1, The 2181th nucleotide A of SEQ ID NO: 1, the 2182th nucleotide A of SEQ ID NO: 1, the 2183th nucleotide G of SEQ ID NO: 1, the 2190th nucleotide C of SEQ ID NO: 1, the 2191st nucleotide T of SEQ ID NO: 1, The 2192th base G of SEQ ID NO: 1, the 2206th base G of SEQ ID NO: 1, the 2495th base G of SEQ ID NO: 1, the 245th base G of SEQ ID NO: 1, base 2582 of SEQ ID NO: 1, base 2591 of base G of SEQ ID NO: 1, base 2603 of base G of SEQ ID NO: 1, base 2605 of base A of SEQ ID NO: 1, base 2610 of base A of SEQ ID NO: 1, The 2626th base G of SEQ ID NO: 1, the 2649th base T of SEQ ID NO: 1, the 2715th base C of SEQ ID NO: 1, the 2726th base T of SEQ ID NO: 1, the 2737th base T of SEQ ID NO: Gt; (Xanthomonas hyacinthi) &lt; / RTI &gt;

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