KR101480154B1 - Method of identifying Xanthomonas hortorum - Google Patents

Method of identifying Xanthomonas hortorum Download PDF

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KR101480154B1
KR101480154B1 KR20120131251A KR20120131251A KR101480154B1 KR 101480154 B1 KR101480154 B1 KR 101480154B1 KR 20120131251 A KR20120131251 A KR 20120131251A KR 20120131251 A KR20120131251 A KR 20120131251A KR 101480154 B1 KR101480154 B1 KR 101480154B1
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seq
hortorum
xanthomonas
polynucleotide consisting
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명인식
이영기
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

본 발명은 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형 동정방법에 관한 것으로 보다 상세하게는 시료로부터 분리한 23S rRNA의 염기를 확인하여 산토모나스 호르토룸(Xanthomonas hortorum)를 동정하는 방법에 관한 것이다. 본 발명의 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형을 동정할 수 있는 방법 및 동정용 프로브를 이용할 경우, 산토모나스 속(Xanthomonas) 으로 부터 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형을 효과적으로 동정 할 수 있다.The present invention relates to a method for identifying Xanthomonas hortorum and a method for identification of the pathogenic type thereof, and more particularly to a method for identifying a base of 23S rRNA isolated from a sample to identify a Santomonas hortorum (Xanthomonas hortorum) . When the method of identifying the Xanthomonas hortorum and the method of identifying the pathotype of the present invention and the identification probe are used, the Xanthomonas hortorum and the hospital type thereof from Xanthomonas It can be identified effectively.

Description

산토모나스 호르토룸 동정방법{Method of identifying Xanthomonas hortorum}{Method of identifying Xanthomonas hortorum}

본 발명은 산토모나스 호르토룸(Xanthomonas hortorum) 동정방법에 관한 것으로 보다 상세하게는 시료로부터 분리한 23S rRNA의 염기를 확인하여 산토모나스 호르토룸(Xanthomonas hortorum)를 동정하는 방법에 관한 것이다.
The present invention relates to a method for identifying Xanthomonas hortorum, and more particularly, to a method for identifying a base of 23S rRNA isolated from a sample to identify a Santomonas hortorum (Xanthomonas hortorum).

산토모나스 속(Xanthomonas)은 토양질소의 탈질에 관여하는 세균이다. 여기에는 병원성 세균으로서 복숭아나무 세균성구멍병, 무 검은빛썩음병, 감귤 궤양병, 벼흰빛잎마름병, 강남콩 불마름병 등을 일으키는 것도 있다.The genus Santomonas (Xanthomonas) is a bacteria involved in the denitrification of soil nitrogen. These include pathogenic bacteria such as peach tree bacterial pericarditis, non-black rot, citrus peptic ulcer disease, rice blight white leaf blight,

산토모나스 호르토룸(Xanthomonas hortorum)은 세균성 점무늬병을 일으키는데, 처음에는 주로 오래된 잎에 수침상 점무늬를 형성하여 점점 커지다가 노란 색의 테두리를 형성하면서 갈색 내지 검은색 점무늬를 형성한다. 심하게 발병하면 잎 전체가 검게 말라 죽으며, 병반에는 검은색 알갱이 등을 형성하지 않아 곰팡이 병에 의한 점무늬병과는 다른 특징을 가진다. Xanthomonas hortorum causes bacterial foliar disease, which initially forms water-borne spots on old leaves, forming brownish to black spots with increasingly larger yellowish rim. When severe onset, the entire leaf is blackened to die, and the lesion does not form black granules, which is different from fungal disease caused by fungal disease.

식물 병원세균의 유전자 동정방법으로는 Dot-blot, 중합효소 연쇄반응(PCR) 등의 방법이 이용된다. 특히 PCR은 Dot-blot 방법보다 시간, 노력, 인력 등이 경제적이기 때문에 최근 널리 사용되고 있는 방법이다. PCR(중합효소 연쇄반응, polymerase chain reaction) 동정 방법은 10~20bp로 구성된 특정 DNA 단편(프라이머)을 세균의 게놈유전자(genomic DNA)에 접촉(annealing)한 후 내열성 DNA 중합효소(Taq polymerase)를 첨가하고 합성반응을 반복적으로 행하게 되면, 프라이머가 결합된 영역이 급속히 증폭하게 되며, 그 PCR 증폭산물을 아가로스 젤(agarose gel) 또는 아크릴아마이드 젤(acrylamide gel)에 전기영동하고 에티디움 브로마이드(ethidium bromide) 및 은 염색(silver stain)으로 DNA를 염색한 후 나타나는 DNA 밴드의 유무 혹은 형태의 차이와 같은 세균종의 DNA 다형성을 동정 동정하는 방법이다. 현재, 이러한 PCR에 의한 세균의 동정법은 인체나 동물의 세균성 질병을 동정에 주로 이용되나, 최근에는 식물 병원균을 동정할 수 있도록 PCR 동정법이 개발되어 병의 동정과 함께 수출입 농산물의 검역에 이용되고 있다. 또한, PCR 동정 방법은 다른 방법에 비해 소요되는 시간이 짧고, 동정에 소요되는 비용이 저렴하며 또한, 많은 샘플을 동시에 검정할 수 있어 경제적이다. 이에 최근에는 산토모나스(Xanthomonas) 동정방법 중에서 유전물질인 핵산을 증폭하는 PCR 동정 방법이 가장 선호되고 있다.Dot-blot, PCR (PCR), etc. are used for gene identification of plant pathogenic bacteria. In particular, PCR is a widely used method because it is economical in terms of time, effort, and manpower than the dot-blot method. PCR (polymerase chain reaction) is a method for identifying a DNA fragment (primer) consisting of 10-20 bp in the genomic DNA of a bacterium and then using heat-resistant DNA polymerase The PCR amplification product is electrophoresed on an agarose gel or an acrylamide gel and ethidium bromide is added to the reaction mixture, bromide, and silver stain. The DNA polymorphism of the bacterium, such as the presence or absence of DNA bands, is identified and identified. Currently, the identification method of bacteria by PCR is mainly used for identification of bacterial diseases in humans and animals. However, in recent years, PCR identification method has been developed to identify plant pathogens and is used for identification of diseases and quarantine of agricultural products . In addition, the PCR identification method is shorter in time than other methods, is low in cost for identification, and is also economical because many samples can be assayed at the same time. Recently, PCR identification method that amplifies nucleic acid which is a genetic material among the identification methods of Santomonas (Xanthomonas) is most preferred.

그러나 기존의 PCR 동정 방법으로는 유전적 유사도로 인한 종간 구분의 어려움이 있어서 이를 극복할 수 있는 해결책이 필요한 실정이다. However, existing PCR identification methods are difficult to classify due to genetic similarity, and a solution to overcome this problem is needed.

기존의 산토모나스 속 미생물 중, 벼흰잎마름병원균(Xanthomonas oryzae pv. oryzae), 세균성점무늬병원균 (Xanthomonas campestris pv. vesicatoria), 십자화과 흑부병원균(Xanthomonas campestris pv. campestris), 감귤류 궤양병 병원균(Xanthomonas axonopodis pv. citri) 및 콩불마름병원균(Xanthomonas axonopodis pv. glycines) 등 다양한 미생물의 동정에 사용되는 개별적인 PCR 프라이머에 대한 연구는 이루어졌으나, 산토모나스 속(Xanthomonas) 으로부터 산토모나스 호르토룸(Xanthomonas hortorum)를 동정해내는 방법에 대하여는 아직까지 연구된 바 없다. 식물병원세균간의 종 또는 병원형 특이적 염기 및 염기 조합을 통해 산토모나스 호르토룸(Xanthomonas hortorum) 및 이의 병원형을 동정할 수 있는 방법 개발이 요구 된다.
Among the existing microorganisms of the genus Santomonas, Xanthomonas oryzae pv. Oryzae, Xanthomonas campestris pv. Vesicatoria, Xanthomonas campestris pv. Campestris, Xanthomonas axonopodis pv. citr and Xanthomonas axonopodis pv. glycines. However, the identification of Xanthomonas hortorum (Xanthomonas hortorum) from the genus Xanthomonas has been studied. The method has not yet been studied. It is required to develop a method capable of identifying the Santomonas hortorum and its hospital type through a species or a hospital type specific base and a base combination among plant pathogenic bacteria.

이에 본 발명의 발명자들은 산토모나스 속(Xanthomonas) 으로부터 산토모나스 호르토룸(Xanthomonas hortorum) 또는 그 아종(병원형)들을 더 정확하고 신속하게 동정을 하기 위해 연구하던 중, 다른 산토모나스 속 세균의 23s rRNA 염기서열과의 차이를 발견하여 이를 이용한 동정방법을 개발하였다.
Accordingly, the inventors of the present invention have been studying to identify the Xanthomonas hortorum or its subspecies from Xanthomonas more accurately and rapidly, and have found that 23s rRNA of other Santomonas bacteria We have discovered the difference between the nucleotide sequences and developed the identification method using them.

따라서 본 발명의 목적은, (a) 시료로부터 23S rRNA를 분리하는 단계; (b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및 (c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸(Xanthomonas hortorum)으로 판단하는 단계를 포함하는 산토모나스 호르토룸(Xanthomonas hortorum) 동정방법을 제공하는 것이다.
Accordingly, an object of the present invention is to provide a method for detecting (i) isolating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is selected from the group consisting of A at position 740, B at position 1668, T at position 2649, C at position 2715, and position 2737 of SEQ ID NO: (Xanthomonas hortorum) in a case where the number of the Xanthomonas hortorum is G in the present invention.

본 발명의 또 다른 목적은, (a) 시료로부터 23S rRNA를 분리하는 단계; (b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및 (c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2050번째 염기가 C, 2531번째 염기가 T, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae)로 판단하는 단계를 포함하는 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정방법을 제공하는 것이다.
Still another object of the present invention is to provide a method for detecting a protein, comprising: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) when the nucleotide sequence identified in step (b) is A, the 740th base is A, the 1357th base is T, the 1668th base is G, the 2050th base is C, Wherein the base is T, the 2715th base is C, and the 2737th base of SEQ ID NO: 1 is G. The method according to any one of claims 1 to 7, wherein the base is Xanthomonas hortorum pv. Hederae, hortorum pv. hederae).

본 발명의 다른 목적은, (a) 시료로부터 23S rRNA를 분리하는 단계; (b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및 (c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni)인 것으로 판단하는 단계를 포함하는 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정방법을 제공하는 것이다.
Another object of the present invention is to provide a method for the detection of 23S rRNA comprising: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is A at position 740, T at position 1357, G at position 1668, T at position 2649, T at position 2715, (Xanthomonas hortorum pv. Pelargoni) comprising the step of judging that the 2737th nucleotide of SEQ ID NO: 1 is a G. Xanthomonas hortorum pv. Pelargoni .

본 발명의 또 다른 목적은, 서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드로 이루어진 군에서 선택된 하나 이상의 폴리뉴클레오티드를 포함하는 산토모나스 호르토룸 (Xanthomonas hortorum) 동정용 프로브를 제공하는 것이다.
It is still another object of the present invention to provide a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, a polynucleotide consisting of 1668th nucleotide G , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th base T of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th nucleotide T of SEQ ID NO: 1, a 2715th nucleotide C , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2737th nucleotide G of SEQ ID NO: 1, and complementary polynucleotides thereof (Xanthomonas hortorum) identification probe comprising at least one polynucleotide selected from the group consisting of A.

본 발명의 또 다른 목적은, 서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1357번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2050번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2531번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드로 이루어진 군에서 선택된 하나 이상의 폴리뉴클레오티드를 포함하는 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정용 프로브를 제공하는 것이다.
Yet another object of the present invention is to provide a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, a polynucleotide consisting of the 1357th base T A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 1668th nucleotide G of SEQ ID NO: 1, a polynucleotide consisting of 2050th nucleotide C A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2531th base T of SEQ ID NO: 1, a polynucleotide consisting of 2049 consecutive DNA sequences comprising 2649th nucleotide T , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising SEQ ID NO: 1, and 20-100 consecutive DNA sequences comprising 2715th nucleotide C of SEQ ID NO: 1 Comprising a polynucleotide, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2737 base G of SEQ ID NO: 1, and a complementary polynucleotide thereof, (Xanthomonas hortorum pv. Hederae) identification probe.

본 발명의 또 다른 목적은, 서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1357번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드로 이루어진 군에서 선택된 하나 이상의 폴리뉴클레오티드를 포함하는 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정용 프로브를 제공하는 것이다.
Yet another object of the present invention is to provide a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, a polynucleotide consisting of the 1357th base T , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th nucleotide G of SEQ ID NO: 1, a 2649th nucleotide T , A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2715th base C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2715th base C of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the complementary polynucleotide of SEQ ID NO: Santo Pseudomonas Hor torum Pella Le Konishi containing polynucleotide to (Xanthomonas hortorum pv. Pelargoni) provides an identification probe.

상기와 같은 목적을 달성하기 위하여 본 발명은 산토모나스 호르토룸(Xanthomonas hortorum) 동정방법을 제공한다. In order to accomplish the above object, the present invention provides a method for identification of Xanthomonas hortorum.

본 발명의 다른 목적을 달성하기 위하여 본 발명은 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정방법을 제공한다. In order to accomplish another object of the present invention, there is provided a method for identifying Santomonas hortorum pv. Hederae.

본 발명의 또 다른 목적을 달성하기 위하여 본 발명은 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정방법을 제공한다.In order to accomplish still another object of the present invention, the present invention provides a method for identification of Santomonas hortorum pv. Pelargoni.

본 발명의 또 다른 목적을 달성하기 위하여 본 발명은 산토모나스 호르토룸(Xanthomonas hortorum) 동정용 프로브를 제공한다.In order to accomplish still another object of the present invention, there is provided a probe for identification of a Xanthomonas hortorum.

본 발명의 또 다른 목적을 달성하기 위하여 본 발명은 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정용 프로브를 제공한다. In order to accomplish still another object of the present invention, there is provided a probe for identification of Santomonas hortorum pv. Hederae.

본 발명의 또 다른 목적을 달성하기 위하여 본 발명은 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정용 프로브를 제공한다.
In order to accomplish still another object of the present invention, there is provided a probe for identification of Santomonas hortorum pv. Pelargoni.

이하, 본 발명을 상세히 설명한다.
Hereinafter, the present invention will be described in detail.

본 발명은 산토모나스 호르토룸(Xanthomonas hortorum) 동정방법에 관한 것이다. The present invention relates to a method for identifying Xanthomonas hortorum.

즉, 본 발명은 산토모나스 속(Xanthomonas) 의 23S rRNA 유전자의 염기조합을 확보하여 산토모나스 호르토룸(Xanthomonas hortorum)를 동정한다.That is, the present invention secures the base combination of the 23S rRNA gene of Xanthomonas to identify the Xanthomonas hortorum.

보다 구체적으로 본 발명의 산토모나스 호르토룸(Xanthomonas hortorum) 동정방법은 (a) 시료로부터 23S rRNA를 분리하는 단계; (b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및 (c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸(Xanthomonas hortorum)으로 판단하는 단계를 포함하는 특징이 있다.
More specifically, the method for identifying Xanthomonas hortorum of the present invention comprises: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is selected from the group consisting of A at position 740, B at position 1668, T at position 2649, C at position 2715, and position 2737 of SEQ ID NO: (G) is G (X), the determination is made as a Santomaras hortorum (Xanthomonas hortorum).

또한 본 발명은 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정방법에 관한 것이다. The present invention also relates to a method for identifying Santomonas hortorum pv. Hederae.

즉, 본 발명은 산토모나스 속(Xanthomonas) 의 23S rRNA 유전자의 염기조합을 확보하여 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae)를 동정한다.That is, the present invention secures the base combination of the 23S rRNA gene of Xanthomonas to identify Xanthomonas hortorum pv. Hederae.

보다 구체적으로 본 발명의 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정방법은 (a) 시료로부터 23S rRNA를 분리하는 단계; (b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및 (c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2050번째 염기가 C, 2531번째 염기가 T, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae)로 판단하는 단계를 포함하는 특징이 있다.
More specifically, the method for identifying Santomonas hortorum pv. Hederae of the present invention comprises the steps of: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) when the nucleotide sequence identified in step (b) is A, the 740th base is A, the 1357th base is T, the 1668th base is G, the 2050th base is C, The second base is T, the 2715th base is C, and the 2737th base of SEQ ID NO: 1 is G, the method comprises the step of determining that the base is Xanthomonas hortorum pv. Hederae.

또한 본 발명은 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정방법에 관한 것이다. The present invention also relates to a method for identification of Santomonas hortorum pv. Pelargoni.

즉, 본 발명은 산토모나스 속(Xanthomonas) 의 23S rRNA 유전자의 염기조합을 확보하여 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 를 동정한다.That is, the present invention secures a base combination of the 23S rRNA gene of Xanthomonas to identify Xanthomonas hortorum pv. Pelargoni.

보다 구체적으로 본 발명의 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정방법은 (a) 시료로부터 23S rRNA를 분리하는 단계; (b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및 (c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni)인 것으로 판단하는 단계를 포함하는 특징이 있다.
More specifically, the present invention provides a method for identifying Xanthomonas hortorum pv. Pelargoni comprising the steps of: (a) separating 23S rRNA from a sample; (b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And (c) the nucleotide sequence identified in step (b) is A at position 740, T at position 1357, G at position 1668, T at position 2649, T at position 2715, And judging that the 2737th base of No. 1 is G, it is judged to be Santomonas hortorum pv. Pelargoni.

또한 본 발명은 산토모나스 호르토룸(Xanthomonas hortorum) 동정용 프로브를 제공한다.The present invention also provides probes for identification of Xanthomonas hortorum.

즉, 본 발명은 산토모나스 속(Xanthomonas) 의 23S rRNA 유전자의 염기조합을 확보하여 산토모나스 호르토룸(Xanthomonas hortorum)를 동정한다.That is, the present invention secures the base combination of the 23S rRNA gene of Xanthomonas to identify the Xanthomonas hortorum.

보다 구체적으로 본 발명의 산토모나스 호르토룸(Xanthomonas hortorum) 동정용 프로브는 서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드로 이루어진 군에서 선택된 하나 이상의 폴리뉴클레오티드를 포함하는 것이 특징이다.
More specifically, the probe for identification of the Xanthomonas hortorum of the present invention comprises a polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th base G of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2649th nucleotide T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising nucleotide 2715 of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2737th nucleotide G of SEQ ID NO: 1, Characterized in that it comprises at least one polynucleotide selected from the group consisting of nucleotides and complementary polynucleotides thereof The.

또한 본 발명은 유전자 염기 조합으로 구성된 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정용 프로브를 제공한다.The present invention also provides a probe for identifying Xanthomonas hortorum pv. Hederae comprising a combination of gene bases.

즉, 본 발명은 산토모나스 속(Xanthomonas) 의 23S rRNA 유전자의 염기조합을 확보하여 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae)를 동정한다.That is, the present invention secures the base combination of the 23S rRNA gene of Xanthomonas to identify Xanthomonas hortorum pv. Hederae.

보다 구체적으로 본 발명의 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정용 프로브는 서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1357번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2050번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2531번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드로 이루어진 군에서 선택된 하나 이상의 폴리뉴클레오티드를 포함하는 것이 특징이다.
More specifically, the probe for identification of Santomonas hortorum pv. Hederae of the present invention comprises 20-100 consecutive DNAs comprising the 740th base A of SEQ ID NO: 1 in the polynucleotide of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1357th base T of SEQ ID NO: 1, 20-100 consecutive DNAs comprising the 1668th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2050th base C of SEQ ID NO: 1, 20-100 consecutive DNAs comprising 2531th nucleotide T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2715th nucleotide C of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2737th nucleotide G of SEQ ID NO: 1, Lt; / RTI > complementary polynucleotide of SEQ ID NO.

또한 본 발명은 유전자 염기 조합으로 구성된 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정용 프로브를 제공한다.In addition, the present invention provides a probe for identification of Xanthomonas hortorum pv. Pelargoni comprising a combination of gene bases.

즉, 본 발명은 산토모나스 속(Xanthomonas) 의 23S rRNA 유전자의 염기조합을 확보하여 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni)를 동정한다.That is, the present invention secures a base combination of the 23S rRNA gene of Xanthomonas to identify Xanthomonas hortorum pv. Pelargoni.

보다 구체적으로 본 발명의 산토모나스 호르토룸 포푸리(Xanthomonas hortorum pv. populi) 동정용 프로브는 서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1357번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드로 이루어진 군에서 선택된 하나 이상의 폴리뉴클레오티드를 포함하는 것이 특징이다.
More specifically, the probe for identifying Santomonas hortorum pv. Puuli of the present invention comprises 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1 in the polynucleotide of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1357th base T of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising the 1668th nucleotide G of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2649th base T of SEQ ID NO: 1, 20-100 consecutive DNA sequences comprising 2715th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2737th base G of SEQ ID NO: 1, and It is characterized by containing the complementary polynucleotide at least one polynucleotide selected from the group consisting of.

본 발명에서 상기 (a) 단계에서 핵산의 분리는 당업계에서 통상적으로 사용되는 방법에 따라 수행될 수 있으며, 상업적으로 판매되는 추출 키트를 이용하여 수행할 수 있다.In the present invention, the nucleic acid may be separated in step (a) according to a method commonly used in the art, and may be carried out using a commercially available extraction kit.

또한 상기 (b) 단계에서 염기서열은 당업계에 공지된 자동 또는 수동의 염기서열 분석방법에 따라 수행될 수 있으며, 바람직하게는 검사체 또는 시료를 대상으로 PCR을 수행하여 23S rRNA에 상응하는 PCR 산물을 제조한 다음 이의 서열을 공지의 염기서열 분석방법에 따라서 분석하여 수행할 수 있다.Also, the base sequence in step (b) may be performed according to automatic or manual base sequence analysis methods known in the art. Preferably, PCR is performed on a test specimen or a sample to perform PCR corresponding to 23S rRNA And the sequence of the product can be analyzed and analyzed according to a known nucleotide sequence analysis method.

상기 (c) 단계에서는 서열번호 1을 기준으로 본 발명의 미생물에서의 판단점에 해당하는 염기서열을 확인하여 본 발명의 미생물에 해당하는지 여부를 판별할 수 있다.
In the step (c), the nucleotide sequence corresponding to the judgment point in the microorganism of the present invention can be identified based on SEQ ID NO: 1 to determine whether the microorganism corresponds to the present invention.

한편, 본 발명은 본 발명의 프로브가 기질상에 집적되어 있는 것을 특징으로 하는 본 발명의 미생물의 검출용 마이크로어레이를 제공한다. 상기 마이크로어레이는 본 발명의 폴리뉴클레오티드를 포함하는 것을 제외하고는 통상적인 마이크로어레이로 이루어지며, 마커로 사용되는 폴리뉴클레오티드를 기관 상에 고정화하여 마이크로어레이를 제조하는 방법은 당업계에 잘 알려져 있다.
Meanwhile, the present invention provides a microarray for detecting microorganisms of the present invention, wherein the probe of the present invention is integrated on a substrate. The microarray consists of a conventional microarray except that it contains a polynucleotide of the present invention, and a method for producing a microarray by immobilizing a polynucleotide used as a marker on an organ is well known in the art.

또한, 본 발명은 본 발명의 미생물의 검출용 마커로서 서열번호 1로 표시되는 염기서열을 가지는 뉴클레오티드에서, 본 발명의 미생물의 판단점에 해당하는 각각의 염기가 치환된 것을 특징으로 하는 검출용 마커를 제공한다. 상기 판단점은 표 3에 기재된 바와 같다.
Further, the present invention is a marker for detecting a microorganism according to the present invention, wherein a nucleotide having a nucleotide sequence shown in SEQ ID NO: 1 is substituted for each of the bases corresponding to the judgment point of the microorganism of the present invention. Lt; / RTI > The above judgment points are as shown in Table 3.

본 발명의 뉴클레오티드, 프로브 또는 마커는 DNA 또는 RNA일 수 있으며, RNA의 경우 서열에 기재된 티민이 우라실로 대체되는 것은 당업자에게 자명하다.
The nucleotide, probe or marker of the present invention may be DNA or RNA, and it is obvious to those skilled in the art that the thymine described in the sequence is replaced by uracil in the case of RNA.

본 발명의 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형들의 동정 방법과 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형들의 동정용 프로브를 이용할 경우, 산토모나스 속(Xanthomonas)으로부터 산토모나스 호르토룸(Xanthomonas hortorum)과 그 아종들을 효과적으로 동정 할 수 있다.
When the identification method of the Xanthomonas hortorum and the hospital types of the present invention and the identification probe of the Xanthomonas hortorum and the hospital types of the present invention are used, it is possible to obtain Santomonas hortorum from Xanthomonas (Xanthomonas hortorum) and its subspecies.

도 1은 산토모나스 캠페스트리스 캠페스트리스(Xanthomonas campestris pv. campestris), 산토모나스 호르토룸 병원형 4종(Xanthomonas hortorum pv. hederae, Xanthomonas hortorum pv. vitians, Xanthomonas hortorum pv. pelargoni, Xanthomonas hortorum pv. taraxaci)의 염기서열 비교분석 결과를 나타낸 것이다.1 is a schematic view showing the results of Xanthomonas hortorum pv. Hederae, Xanthomonas hortorum pv. Vitians, Xanthomonas hortorum pv. Pelargoni, Xanthomonas hortorum pv. taraxaci).

이하, 본 발명을 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by way of examples.

단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

<< 실시예Example 1> 1>

X. X. hortorumhortorum 및 그  And 병원형들의Hospitals 23s  23s rRNArRNA 분석 analysis

하기 실험 방법을 통하여, Xanthomonas속 세균에 대한 동정용 프로브를 설계하였다. 본 발명에서 사용한 전체 RNA 분리 및 염기서열 증폭, 분석방법은 다음과 같다.
Through the following experimental procedure, a probe for identification of bacteria belonging to the genus Xanthomonas was designed. The total RNA isolation, base sequence amplification and analysis methods used in the present invention are as follows.

<1-1> 균들의 23s <1-1> 23s of fungi rRNArRNA 증폭 Amplification

X. hortorum pv. hederae, X. hortorum pv. vitians, X. hortorum pv. pelargoni, X. hortorum pv. taraxaci의 DNA를 분리한 후, 산토모나스(Xanthomonas) 속 세균의 23s rRNA를 분리할 수 있는 하기 [표 1]과 같은 프라이머 쌍들을 이용하여, PCR을 수행하였다.X. hortorum pv. hederae, X. hortorum pv. vitians, X. hortorum pv. pelargoni, X. hortorum pv. The DNA of taraxaci was isolated and PCR was performed using primer pairs as shown in Table 1 below, which can isolate 23s rRNA of bacteria of the genus Xanthomonas.

프라이머 명칭Name of the primer 프라이머 서열Primer sequence Xan23SF5 (전방향)Xan23SF5 (omnidirectional) 5‘-TGGTCAAGCCGCACGGATCATTAGTAT-3’5'-TGGTCAAGCCGCACGGATCATTAGTAT-3 ' Xan23SR6 (역방향)Xan23SR6 (reverse direction) 5‘-ACGTGGATAGCCTGCGAAAAGTGTC-3’5'-ACGTGGATAGCCTGCGAAAAGTGTC-3 ' Xan23SF7 (전방향)Xan23SF7 (Omnidirectional) 5‘-GAGACCGCCCCAGTCAAACTAC-3’5'-GAGACCGCCCCAGTCAAACTAC-3 ' Xan23SR7 (역방향)Xan23SR7 (reverse direction) 5‘-ACCTTTTGTATAATGGGTCAACG-3’ 5'-ACCTTTTGTATAATGGGTCAACG-3 ' Xan23SF9 (전방향)Xan23SF9 (Omnidirectional) 5‘-GTCAAGCCGCACGGATCATTAGTAT-3’5'-GTCAAGCCGCACGGATCATTAGTAT-3 ' Xan23SR9 (역방향)Xan23SR9 (reverse direction) 5-GTCGGGGAGCTGGCAACAAG-’35-GTCGGGGAGCTGGCAACAAG -'3

PCR 반응은 분리한 전체 DNA 20 ng, 다운스트림 프라이머(downstream primer) 10 pmole , 업스트림프라이머(upstream primer) 10 pmole ,Taq DNA polymerase 0.2 U , 10배 중합효소연쇄반응 완충액(100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl2, pH 8.3) 5 ㎕를 첨가하고 증류수를 첨가하여 반응액을 50 ㎕로 만들었다. The PCR reaction consisted of 20 ng of isolated total DNA, 10 pmoles of downstream primer, 10 pmole of upstream primer, 0.2 U of Taq DNA polymerase, 10 times Polymerase chain reaction buffer (100 mM Tris-HCl, 500 mM KCl, 15 mM MgCl 2 , pH 8.3) was added and distilled water was added to make 50 μl of reaction solution.

이 반응액을 95℃에서 5분 동안 가열 후, 95℃ 30 초, 65℃ 60 초, 72℃ 180초로 30 회 증폭시켰으며 마지막에는 72℃에서 10 분간 PCR 기계 (DNA Engine PTC-200)에서 처리하였다. 증폭 후 PCR 산물은 1× TBE 완충액과 1.5 % 아가로즈 겔을 사용하여 전기영동한 후 ethidium bromide로 염색한 후 ultraviolet lamp에서 확인하였다.
The reaction solution was heated at 95 ° C. for 5 minutes and then amplified 30 times at 95 ° C. for 30 seconds, 65 ° C. for 60 seconds, and 72 ° C. for 180 seconds. Finally, the reaction solution was treated at 72 ° C. for 10 minutes with a PCR machine (DNA Engine PTC-200) Respectively. After amplification, the PCR product was electrophoresed using 1 × TBE buffer and 1.5% agarose gel, stained with ethidium bromide, and then confirmed by ultraviolet lamp.

<1-2> 증폭한 23s rRNA 서열 분석<1-2> Amplified 23s rRNA sequence analysis

상기 실시예 <1-1>에서 얻은 PCR 산물들의 염기서열은 BioEdit Sequence Alignment Editor를 이용하여 분석하였다.The nucleotide sequences of the PCR products obtained in Example <1-1> were analyzed using BioEdit Sequence Alignment Editor.

분석한 후, X. campestris pv. campestris 의 23s rRNA 염기서열과 비교해보았다. X. campestris pv. campestris 23s rRNA 염기서열은 Genbank에 게재된 정보(NC_003902.1)를 이용하였다. 본 실험에서 사용한 균들의 23s rRNA 서열들은 하기 [표 2]와 같이 서열번호로 정리하였다.
After analysis, X. campestris pv. We compared the 23s rRNA sequences of campestris. X. campestris pv. For the campestris 23s rRNA sequence, the information (NC_003902.1) published in Genbank was used. The 23s rRNA sequences of the bacteria used in this experiment are summarized in the sequence numbers as shown in Table 2 below.

서열번호SEQ ID NO: 균Germ 1One X. campestris pv. campestris X. campestris pv. campestris 22 X. hortorum pv. hederaeX. hortorum pv. hederae 33 X. hortorum pv. vitiansX. hortorum pv. vitians 44 X. hortorum pv. pelargoniX. hortorum pv. pelargoni 55 X. hortorum pv. taraxaciX. hortorum pv. taraxaci

<실시예 2> &Lt; Example 2 >

균들간의 23s rRNA 서열 비교 분석Comparative analysis of 23s rRNA sequence between bacteria

상기 [표 2]의 서열번호 1 내지 5 간에 염기서열 차이를 비교해보았다. 염기서열은 BioEdit Sequence Alignment Editor를 이용하여 비교하였다.The nucleotide sequence differences between SEQ ID NOS: 1 to 5 of Table 2 were compared. The nucleotide sequences were compared using the BioEdit Sequence Alignment Editor.

분석 결과는 [도 1]에 기재하였고, X. campestris pv. campestris의 염기서열(서열번호 1)을 기준으로 X. hortorum 병원형과의 염기서열 차이가 있는 위치를 하기 [표 3]에 정리하였다.
The results of the analysis are shown in Fig. 1, and X. campestris pv. Table 3 summarizes the position of nucleotide sequence differences with the type of X. hortorum based on the nucleotide sequence of Campestris (SEQ ID NO: 1).

균Germ 위치 / 바뀐 염기Position / changed base X. hortorum pv. hederaeX. hortorum pv. hederae 740/a740 / a 1668/g 1668 g 2649/t 2649 / t 2715/c 2715 / c 2737/g2737 / g 1357/t1357 / t 2050/c2050 / c 2531/t2531 / t X. hortorum pv. vitiansX. hortorum pv. vitians 740/a740 / a 1668/g 1668 g 2649/t 2649 / t 2715/c 2715 / c 2737/g2737 / g X. hortorum pv. pelargoniX. hortorum pv. pelargoni 740/a740 / a 1668/g 1668 g 2649/t 2649 / t 2715/c 2715 / c 2737/g2737 / g 1357/t1357 / t X. hortorum pv. taraxaciX. hortorum pv. taraxaci 740/a740 / a 1668/g 1668 g 2649/t 2649 / t 2715/c 2715 / c 2737/g2737 / g

상기 표에서 위치를 나타내는 숫자는 서열번호 1의 n 번째 자리를 의미한다. 따라서 상기 [표 3]에 나타난 염기의 차이를 이용하여 균들을 동정할 수 있다.
The number indicating the position in the above table means the n-th digit of SEQ ID NO: 1. Therefore, the bacterium can be identified using the difference of the bases shown in [Table 3] above.

이상 살펴본 바와 같이 본 발명의 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형 동정 방법과 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형 동정용 프로브를 이용할 경우, 산토모나스 속(Xanthomonas) 으로 부터 산토모나스 호르토룸(Xanthomonas hortorum) 및 그 병원형을 동정할 수 있으므로, 산업상 이용가능성이 높다.As described above, when using the Xanthomonas hortorum and its hospital-type identification method and the Xanthomonas hortorum and the hospital-type identification probe of the present invention, it is possible to use Santomonas hortorum, Xanthomonas hortorum, It is possible to identify the Monas Hortorum (Xanthomonas hortorum) and its type of hospital, and thus the possibility of industrial use is high.

<110> REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION) <120> Method of identifying Xanthomonas hortorum <130> NP12-0060 <160> 11 <170> KopatentIn 2.0 <210> 1 <211> 2883 <212> DNA <213> X. campestris pv. campestris NC_003902 <400> 1 atggtcaagc cgcacggatc attagtatca gttagctcaa tacattgctg tacttacaca 60 cctgacctat caaccacata gtctatatgg ttcctttagg gggcttgtgc cccgggaagt 120 ctcatcttga ggcgcgcttc ccgcttagat gctttcagcg gttatcgctt ccgaacatag 180 ctacccggca atgccactgg cgtgacaacc ggaacaccag aggttcgtcc actccggtcc 240 tctcgtacta ggagcagccc ctctcaaact tccaacgccc atggcagata gggaccgaac 300 tgtctcacga cgttctgaac ccagctcgcg taccacttta aatggcgaac agccataccc 360 ttgggaccga ctacagcccc aggatgtgat gagccgacat cgaggtgcca aacaccgccg 420 tcgatatgaa ctcttgggcg gtatcagcct gttatccccg gagtaccttt tatccgttga 480 gcgatggccc ttccatacag aaccaccgga tcactaagac ctactttcgt acctgcttga 540 tccgtcgatc ttgcagtcaa gcacgcttat gcctttgcac acagtgcgcg atgtccgacc 600 gcgctgagcg taccttcgtg ctcctccgtt actctttagg aggagaccgc cccagtcaaa 660 ctacccacca tacacggtcc ctgatccgga taacggatct aggttagaac gtcaagcacg 720 acagggtggt atttcaaggt tggctccact gcagctagcg ccacagtttc atagcctccc 780 acctatccta cacagacgaa ctcaacgttc agtgtaaagc tatagtaaag gttcacgggg 840 tctttccgtc ttgccacggg aacgctgcat cttcacagcg atttcaattt cactgagtct 900 cgggtggaga cagcgccgct gtcgttacgc cattcgtgca ggtcggaact tacccgacaa 960 ggaatttcgc taccttagga ccgttatagt tacggccgcc gtttactggg gcttcgatca 1020 agagcttcgc cttgcggctg accccatcaa ttaaccttcc agcaccgggc aggcgtcaca 1080 ccctatacgt ccactttcgt gtttgcagag tgctgtgttt ttgataaaca gtcgcagcgg 1140 cctggtttct gcgaccctct tcagctatag ctcgcatgag ccaccaaaaa gggtgcacct 1200 tctcccgaag ttacggtgcc atgttgccta gttccttcac ccgagttctc tcaagcgcct 1260 gagaattctc atcctaccca cctgtgtcgg tttacggtac ggtcttcgtg agctgaagct 1320 taggagcttt tcctggaagc gtggtatcag tgacttcgcc ataaaggctc gtctcggtgc 1380 tcggtcttaa aggatcccgg atttgccaaa gatccaaacc taccgccttt ccccgggaca 1440 accaacgccc ggtacaccta accttctccg tccctccatc gcactcacgc gaggtgcagg 1500 aatattaacc tgcttcccat cgactacggc tttcgccctc gccttaggga ccgactaacc 1560 ctgcgtcgat taacgttgcg caaggaaacc ttgggctttc ggcgtgcggg cttttcaccc 1620 gcattatcgt tactcatgtc agcattcgca cttccgatac ctccagcaga cttctcaatc 1680 caccttcgca ggcttacgga acgctcctct accgcgcata aaaccgaagt tttacgcacc 1740 ccaagcttcg gttcactgct tagccccgtt aaatcttccg cgcagaccga ctcgaccagt 1800 gagctattac gctttcttta aagggtggct gcttctaagc caacctcctg gctgtctatg 1860 cctttccaca tcgttttcca cttagcagtg aatttgggac cttagctgtg ggtctgggtt 1920 gtttcccttt tcacgacgga cgttagcacc cgccgtgtgt ctcccggata gtacgtactg 1980 gtattcggag tttgcaatgg tttggtaagt cgcgatgacc ccctagccat aacagtgctc 2040 tacccccagt agtattcgtc cgaggcgcta cctaaatagc tttcgaggag aaccagctat 2100 ctccgggttc gattagcttt tcactcctaa tcacagctca tccccgtctt ttgcaacaga 2160 cgtgggttcg ggcctccagt acctgttacg gcaccttcac cctggccatg actagatcac 2220 ccggtttcgg gtctactgcc cgcgactatg cgcccttatc agactcggtt tcccttcgcc 2280 tcccctatac ggttaagctt gccacgaaca gtaagtcgct gacccattat acaaaaggta 2340 cgcagtcact cttgcgagct cctactgctt gtacgcacac ggtttcagga tctatttcac 2400 tcccctctcc ggggttcttt tcgcctttcc ctcacggtac tggttcacta tcggtcggtc 2460 aggagtattt agccttggag gatggtcccc ccatattcag acagggtttc acgtgccccg 2520 ccctactcgt cttcactgga gtggcccttt taaatacagg gctatcacct tctatggcca 2580 atctttccag attgtttttc taaagccatt ccagcttaag ggctgttccc cgttcgctcg 2640 tcactactca gggaatctcg gttgatttct tttcctccgg ttacttagat atttcagttc 2700 accgggttcg cttcaagcag ctatgaattc actgcaagat actgccgaag cagtgggttt 2760 ccccattcgg atattgccgg atcaaagctt gttgccagct ccccgacact tttcgcaggc 2820 taccacgtcc ttcatcgcct ctgaccgcct aggcatccac cgtgtgcgct tattcgcttg 2880 acc 2883 <210> 2 <211> 2716 <212> DNA <213> X hortorum pv. vitians BC2575 <400> 2 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact tcgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagtagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgtcttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 3 <211> 2716 <212> DNA <213> X hortorum pv. hederae BC2624 <400> 3 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact ttgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagcagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgttttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 4 <211> 2716 <212> DNA <213> X hortorum pv. pelargonii BC2602 <400> 4 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact ttgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagtagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgtcttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 5 <211> 2716 <212> DNA <213> X hortorum pv. taraxaci BC2606 <400> 5 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact tcgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagtagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgtcttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 6 <211> 27 <212> DNA <213> Xan23SF5 <400> 6 tggtcaagcc gcacggatca ttagtat 27 <210> 7 <211> 25 <212> DNA <213> Xan23SR6 <400> 7 acgtggatag cctgcgaaaa gtgtc 25 <210> 8 <211> 22 <212> DNA <213> Xan23SF7 <400> 8 gagaccgccc cagtcaaact ac 22 <210> 9 <211> 23 <212> DNA <213> Xan23SR7 <400> 9 accttttgta taatgggtca acg 23 <210> 10 <211> 25 <212> DNA <213> Xan23SF9 <400> 10 gtcaagccgc acggatcatt agtat 25 <210> 11 <211> 20 <212> DNA <213> Xan23SR9 <400> 11 gtcggggagc tggcaacaag 20 <110> REPUBLIC OF KOREA (MANAGEMENT: RURAL DEVELOPMENT ADMINISTRATION) <120> Method of identifying Xanthomonas hortorum <130> NP12-0060 <160> 11 <170> Kopatentin 2.0 <210> 1 <211> 2883 <212> DNA <213> X. campestris pv. campestris NC_003902 <400> 1 atggtcaagc cgcacggatc attagtatca gttagctcaa tacattgctg tacttacaca 60 cctgacctat caaccacata gtctatatgg ttcctttagg gggcttgtgc cccgggaagt 120 ctcatcttga ggcgcgcttc ccgcttagat gctttcagcg gttatcgctt ccgaacatag 180 ctacccggca atgccactgg cgtgacaacc ggaacaccag aggttcgtcc actccggtcc 240 tctcgtacta ggagcagccc ctctcaaact tccaacgccc atggcagata gggaccgaac 300 tgtctcacga cgttctgaac ccagctcgcg taccacttta aatggcgaac agccataccc 360 ttgggaccga ctacagcccc aggatgtgat gagccgacat cgaggtgcca aacaccgccg 420 tcgatatgaa ctcttgggcg gtatcagcct gttatccccg gagtaccttt tatccgttga 480 gcgatggccc ttccatacag aaccaccgga tcactaagac ctactttcgt acctgcttga 540 tccgtcgatc ttgcagtcaa gcacgcttat gcctttgcac acagtgcgcg atgtccgacc 600 gcgctgagcg taccttcgtg ctcctccgtt actctttagg aggagaccgc cccagtcaaa 660 ctacccacca tacacggtcc ctgatccgga taacggatct aggttagaac gtcaagcacg 720 acagggtggt atttcaaggt tggctccact gcagctagcg ccacagtttc atagcctccc 780 acctatccta cacagacgaa ctcaacgttc agtgtaaagc tatagtaaag gttcacgggg 840 tctttccgtc ttgccacggg aacgctgcat cttcacagcg atttcaattt cactgagtct 900 cgggtggaga cagcgccgct gtcgttacgc cattcgtgca ggtcggaact tacccgacaa 960 ggaatttcgc taccttagga ccgttatagt tacggccgcc gtttactggg gcttcgatca 1020 agagcttcgc cttgcggctg accccatcaa ttaaccttcc agcaccgggc aggcgtcaca 1080 ccctatacgt ccactttcgt gtttgcagag tgctgtgttt ttgataaaca gtcgcagcgg 1140 cctggtttct gcgaccctct tcagctatag ctcgcatgag ccaccaaaaa gggtgcacct 1200 tctcccgaag ttacggtgcc atgttgccta gttccttcac ccgagttctc tcaagcgcct 1260 gagaattctc atcctaccca cctgtgtcgg tttacggtac ggtcttcgtg agctgaagct 1320 taggagcttt tcctggaagc gtggtatcag tgacttcgcc ataaaggctc gtctcggtgc 1380 tcggtcttaa aggatcccgg atttgccaaa gatccaaacc taccgccttt ccccgggaca 1440 accaacgccc ggtacaccta accttctccg tccctccatc gcactcacgc gaggtgcagg 1500 aatattaacc tgcttcccat cgactacggc tttcgccctc gccttaggga ccgactaacc 1560 ctgcgtcgat taacgttgcg caaggaaacc ttgggctttc ggcgtgcggg cttttcaccc 1620 gcattatcgt tactcatgtc agcattcgca cttccgatac ctccagcaga cttctcaatc 1680 caccttcgca ggcttacgga acgctcctct accgcgcata aaaccgaagt tttacgcacc 1740 ccaagcttcg gttcactgct tagccccgtt aaatcttccg cgcagaccga ctcgaccagt 1800 gagctattac gctttcttta aagggtggct gcttctaagc caacctcctg gctgtctatg 1860 cctttccaca tcgttttcca cttagcagtg aatttgggac cttagctgtg ggtctgggtt 1920 gtttcccttt tcacgacgga cgttagcacc cgccgtgtgt ctcccggata gtacgtactg 1980 gtattcggag tttgcaatgg tttggtaagt cgcgatgacc ccctagccat aacagtgctc 2040 tacccccagt agtattcgtc cgaggcgcta cctaaatagc tttcgaggag aaccagctat 2100 ctccgggttc gattagcttt tcactcctaa tcacagctca tccccgtctt ttgcaacaga 2160 cgtgggttcg ggcctccagt acctgttacg gcaccttcac cctggccatg actagatcac 2220 ccggtttcgg gtctactgcc cgcgactatg cgcccttatc agactcggtt tcccttcgcc 2280 tcccctatac ggttaagctt gccacgaaca gtaagtcgct gacccattat acaaaaggta 2340 cgcagtcact cttgcgagct cctactgctt gtacgcacac ggtttcagga tctatttcac 2400 tcccctctcc ggggttcttt tcgcctttcc ctcacggtac tggttcacta tcggtcggtc 2460 aggagtattt agccttggag gatggtcccc ccatattcag acagggtttc acgtgccccg 2520 ccctactcgt cttcactgga gtggcccttt taaatacagg gctatcacct tctatggcca 2580 atctttccag attgtttttc taaagccatt ccagcttaag ggctgttccc cgttcgctcg 2640 tcactactca gggaatctcg gttgatttct tttcctccgg ttacttagat atttcagttc 2700 accgggttcg cttcaagcag ctatgaattc actgcaagat actgccgaag cagtgggttt 2760 ccccattcgg atattgccgg atcaaagctt gttgccagct ccccgacact tttcgcaggc 2820 taccacgtcc ttcatcgcct ctgaccgcct aggcatccac cgtgtgcgct tattcgcttg 2880 acc 2883 <210> 2 <211> 2716 <212> DNA <213> X hortorum pv. vitians BC2575 <400> 2 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact tcgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagtagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgtcttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 3 <211> 2716 <212> DNA <213> X hortorum pv. hederae BC2624 <400> 3 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact ttgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagcagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgttttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 4 <211> 2716 <212> DNA <213> X hortorum pv. pelargonii BC2602 <400> 4 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact ttgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagtagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgtcttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 5 <211> 2716 <212> DNA <213> X hortorum pv. taraxaci BC2606 <400> 5 acacacctga cctatcaacc acatagtcta tatggttcct ttagggggct tgtgccccgg 60 gaagtctcat cttgaggcgc gcttcccgct tagatgcttt cagcggttat cgcttccgaa 120 catagctacc cggcaatgcc actggcgtga caaccggaac accagaggtt cgtccactcc 180 ggtcctctcg tactaggagc agcccctctc aaacttccaa cgcccatggc agatagggac 240 cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaaatgg cgaacagcca 300 tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg tgccaaacac 360 cgccgtcgat atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc 420 gttgagcgat ggcccttcca tacagaacca ccggatcact aagacctact ttcgtacctg 480 cttgatccgt cgatcttgca gtcaagcacg cttatgcctt tgcacacagt gcgcgatgtc 540 cgaccgcgct gagcgtacct tcgtgctcct ccgttactct ttaggaggag accgccccag 600 tcaaactacc caccatacac ggtccctgat ccggataacg gatctaggtt agaacgtcaa 660 gcacgacagg gtggtatttc aaggatggct ccactgcagc tagcgccaca gtttcatagc 720 ctcccaccta tcctacacag acgaactcaa cgttcagtgt aaagctatag taaaggttca 780 cggggtcttt ccgtcttgcc acgggaacgc tgcatcttca cagcgatttc aatttcactg 840 agtctcgggt ggagacagcg ccgctgtcgt tacgccattc gtgcaggtcg gaacttaccc 900 gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta ctggggcttc 960 gatcaagagc ttcgccttgc ggctgacccc atcaattaac cttccagcac cgggcaggcg 1020 tcacacccta tacgtccact ttcgtgtttg cagagtgctg tgtttttgat aaacagtcgc 1080 agcggcctgg tttctgcgac cctcttcagc tatagctcgc atgagccacc aaaaagggtg 1140 caccttctcc cgaagttacg gtgccatgtt gcctagttcc ttcacccgag ttctctcaag 1200 cgcctgagaa ttctcatcct acccacctgt gtcggtttac ggtacggtct tcgtgagctg 1260 aagcttagga gcttttcctg gaagcgtggt atcagtgact tcgccataaa ggctcgtctc 1320 ggtgctcggt cttaaaggat cccggatttg ccaaagatcc aaacctaccg cctttccccg 1380 ggacaaccaa cgcccggtac acctaacctt ctccgtccct ccatcgcact cacgcgaggt 1440 gcaggaatat taacctgctt cccatcgact acggctttcg ccctcgcctt agggaccgac 1500 taaccctgcg tcgattaacg ttgcgcaagg aaaccttggg ctttcggcgt gcgggctttt 1560 cacccgcatt atcgttactc atgtcagcat tcgcacttcc gatacctcca gcggacttct 1620 caatccacct tcgcaggctt acggaacgct cctctaccgc gcataaaacc gaagttttac 1680 gcaccccaag cttcggttca ctgcttagcc ccgttaaatc ttccgcgcag accgactcga 1740 ccagtgagct attacgcttt ctttaaaggg tggctgcttc taagccaacc tcctggctgt 1800 ctatgccttt ccacatcgtt ttccacttag cagtgaattt gggaccttag ctgtgggtct 1860 gggttgtttc ccttttcacg acggacgtta gcacccgccg tgtgtctccc ggatagtacg 1920 tactggtatt cggagtttgc aatggtttgg taagtcgcga tgacccccta gccataacag 1980 tgctctaccc ccagtagtat tcgtccgagg cgctacctaa atagctttcg aggagaacca 2040 gctatctccg ggttcgatta gcttttcact cctaatcaca gctcatcccc gtcttttgca 2100 acagacgtgg gttcgggcct ccagtacctg ttacggcacc ttcaccctgg ccatgactag 2160 atcacccggt ttcgggtcta ctgcccgcga ctatgcgccc ttatcagact cggtttccct 2220 tcgcctcccc tatacggtta agcttgccac gaacagtaag tcgctgaccc attatacaaa 2280 aggtacgcag tcactcttgc gagctcctac tgcttgtacg cacacggttt caggatctat 2340 ttcactcccc tctccggggt tcttttcgcc tttccctcac ggtactggtt cactatcggt 2400 cggtcaggag tatttagcct tggaggatgg tccccccata ttcagacagg gtttcacgtg 2460 ccccgcccta ctcgtcttca ctggagtggc ccttttaaat acagggctat caccttctat 2520 ggccaatctt tccagattgt ttttctaaag ccattccagc ttaagggctg ttccccgttc 2580 gctcgtcact acttagggaa tctcggttga tttcttttcc tccggttact tagatatttc 2640 agttcaccgg gttcgcttcc agcagctatg aattcactgc aggatactgc cgaagcagtg 2700 ggtttcccca ttcgga 2716 <210> 6 <211> 27 <212> DNA <213> Xan23SF5 <400> 6 tggtcaagcc gcacggatca ttagtat 27 <210> 7 <211> 25 <212> DNA <213> Xan23SR6 <400> 7 acgtggatag cctgcgaaaa gtgtc 25 <210> 8 <211> 22 <212> DNA <213> Xan23SF7 <400> 8 gagaccgccc cagtcaaact ac 22 <210> 9 <211> 23 <212> DNA <213> Xan23SR7 <400> 9 accttttgta taatgggtca acg 23 <210> 10 <211> 25 <212> DNA <213> Xan23SF9 <400> 10 gtcaagccgc acggatcatt agtat 25 <210> 11 <211> 20 <212> DNA <213> Xan23SR9 <400> 11 gtcggggagc tggcaacaag 20

Claims (10)

(a) 시료로부터 23S rRNA를 분리하는 단계;
(b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및
(c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸(Xanthomonas hortorum)으로 판단하는 단계를 포함하는 산토모나스 호르토룸(Xanthomonas hortorum) 동정방법.
(a) separating 23S rRNA from the sample;
(b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And
(c) the nucleotide sequence identified in step (b) is A at position 740, B at position 1668, T at position 2649, T at position 2715, position 2737 of SEQ ID NO: 1 G, and determining it as the Xanthomonas hortorum. &Lt; Desc / Clms Page number 20 &gt;
(a) 시료로부터 23S rRNA를 분리하는 단계;
(b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및
(c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2050번째 염기가 C, 2531번째 염기가 T, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae)로 판단하는 단계를 포함하는 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정방법.
(a) separating 23S rRNA from the sample;
(b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And
(c) when the nucleotide sequence identified in step (b) is A, the 740th base is A, the 1357th base is T, the 1668th base is G, the 2050th base is C, the 2531th base is T, Wherein the base is T, the 2715th base is C, and the 2737th base of SEQ ID NO: 1 is G. The method according to any one of claims 1 to 7, wherein the base is Xanthomonas hortorum pv. Hederae, pv. hederae) identification method.
(a) 시료로부터 23S rRNA를 분리하는 단계;
(b) 상기 (a) 단계에서 분리한 23S rRNA의 염기서열을 확인하는 단계; 및
(c) 상기 (b) 단계에서 확인된 염기서열이 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G인 경우 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni)인 것으로 판단하는 단계를 포함하는 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정방법.
(a) separating 23S rRNA from the sample;
(b) confirming the nucleotide sequence of the 23S rRNA isolated in the step (a); And
(c) the nucleotide sequence identified in step (b) is A, the 740th base is A, the 1357th base is T, the 1668th base is G, the 2649th base is T, the 2715th base is C, 1, and determining that the 2737th base is G, it is determined to be Santomonas hortorum pv. Pelargoni.
서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드 모두를 포함하는 산토모나스 호르토룸(Xanthomonas hortorum) 동정용 프로브 조성물.
A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive sequences comprising the 1668th base G of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2649th base T of SEQ ID NO: 1, 20-100 consecutive sequences comprising 2715th nucleotide C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2737th base G of SEQ ID NO: 1, and a complementary polynucleotide thereof, as well as the Xanthomonas &lt; RTI ID = 0.0 &gt; hortorum) identification probes.
서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1357번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2050번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2531번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드 모두를 포함하는 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 동정용 프로브 조성물.
A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive sequences comprising the 1357th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th base G of SEQ ID NO: 1, a 20-100 consecutive sequences comprising the 2050th base C of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2531 base T of SEQ ID NO: 1, 20-100 consecutive sequences comprising 2649th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 2715th base C of SEQ ID NO: 1, A probe for identification of Xanthomonas hortorum pv. Hederae containing both polynucleotide consisting of 20-100 consecutive DNA sequences containing the 2737th base G of No. 1 and complementary polynucleotides thereof Composition.
서열번호 1로 이루어지는 폴리뉴클레오티드에서, 서열번호 1의 740번째 염기 A를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1357번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 1668번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2649번째 염기 T를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2715번째 염기 C를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드, 서열번호 1의 2737번째 염기 G를 포함하는 20-100개의 연속적인 DNA 서열로 구성되는 폴리뉴클레오티드 및 이들의 상보적인 폴리뉴클레오티드 모두를 포함하는 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 동정용 프로브 조성물.
A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 740th base A of SEQ ID NO: 1, a polynucleotide consisting of 20-100 consecutive sequences comprising the 1357th base T of SEQ ID NO: 1, A polynucleotide consisting of 20-100 consecutive DNA sequences comprising the 1668th base G of SEQ ID NO: 1, a 20-100 consecutive sequences comprising the 2649th base T of SEQ ID NO: 1 A polynucleotide consisting of 20-100 consecutive DNA sequences comprising 2715th base C of SEQ ID NO: 1, 20-100 consecutive sequences comprising 2737th base G of SEQ ID NO: 1 Lt; RTI ID = 0.0 &gt; Xanthomonas hortor &lt; / RTI &gt; (including both polynucleotides consisting of DNA sequences of SEQ ID NO: um pv. pelargoni).
제4항 내지 제6항 중 어느 한 항의 프로브 조성물이 기질상에 집적되어 있는 것을 특징으로 하는 마이크로어레이.
A microarray characterized in that the probe composition of any one of claims 4 to 6 is integrated on a substrate.
서열번호 1로 표시되는 염기서열을 가지는 뉴클레오티드에서, 서열번호 1의 740번째 염기가 A, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G로 치환된 것을 특징으로 하는 산토모나스 호르토룸(Xanthomonas hortorum) 검출용 마커.
In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 740th base of SEQ ID NO: 1 is A, the 1668th base is G, the 2649th base is T, the 2715th base is C, the 2737th base of SEQ ID NO: 1 is G (Xanthomonas hortorum). &Lt; / RTI &gt;
서열번호 1로 표시되는 염기서열을 가지는 뉴클레오티드에서, 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2050번째 염기가 C, 2531번째 염기가 T, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G로 치환된 것을 특징으로 하는 산토모나스 호르토룸 헤데레(Xanthomonas hortorum pv. hederae) 검출용 마커.
In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 740th base of SEQ ID NO: 1 is A, the 1357th base is T, the 1668th base is G, the 2050th base is C, the 2531st base is T, , T at position 2715, C at position 2715, and G at position 2737 of SEQ ID NO: 1.
서열번호 1로 표시되는 염기서열을 가지는 뉴클레오티드에서, 서열번호 1의 740번째 염기가 A, 1357번째 염기가 T, 1668번째 염기가 G, 2649번째 염기가 T, 2715번째 염기가 C, 서열번호 1의 2737번째 염기가 G로 치환된 것을 특징으로 하는 산토모나스 호르토룸 펠라르고니(Xanthomonas hortorum pv. pelargoni) 검출용 마커.In the nucleotide having the nucleotide sequence shown in SEQ ID NO: 1, the 740th base is A, the 1357th base is T, the 1668th base is G, the 2649th base is T, the 2715th base is C, Wherein the 2737th nucleotide of SEQ ID NO: 1 is substituted with G. The marker for detecting Xanthomonas hortorum pv. Pelargoni.
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Citations (2)

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Publication number Priority date Publication date Assignee Title
KR20030087911A (en) * 2002-05-09 2003-11-15 (주)메디제네스 DNA Probe Derived from 23S rRNA Gene for Detection of Infectious Bacteria
KR20120053792A (en) * 2010-11-18 2012-05-29 대한민국(농촌진흥청장) Primer set of polymerase chain reaction for detecting the genus xanthomonas and method for detecting the genus xanthomonas using the same

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030087911A (en) * 2002-05-09 2003-11-15 (주)메디제네스 DNA Probe Derived from 23S rRNA Gene for Detection of Infectious Bacteria
KR20120053792A (en) * 2010-11-18 2012-05-29 대한민국(농촌진흥청장) Primer set of polymerase chain reaction for detecting the genus xanthomonas and method for detecting the genus xanthomonas using the same

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