KR101850852B1 - Microfluidic channel device and method for using the same - Google Patents

Microfluidic channel device and method for using the same Download PDF

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Publication number
KR101850852B1
KR101850852B1 KR1020160002583A KR20160002583A KR101850852B1 KR 101850852 B1 KR101850852 B1 KR 101850852B1 KR 1020160002583 A KR1020160002583 A KR 1020160002583A KR 20160002583 A KR20160002583 A KR 20160002583A KR 101850852 B1 KR101850852 B1 KR 101850852B1
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South Korea
Prior art keywords
channel
cell
solution
cell trap
cells
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KR1020160002583A
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Korean (ko)
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KR20170083293A (en
Inventor
이태린
이철균
서형권
Original Assignee
재단법인차세대융합기술연구원
인하대학교 산학협력단
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Priority to KR1020160002583A priority Critical patent/KR101850852B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Abstract

The present disclosure relates to a microfluidic channel device in which a solution containing cells flows in a predetermined direction, comprising: a main channel; 1. A cell trap provided in a main channel, the cell trap comprising: a cell trap having an inner side surface, an outer side surface and an inlet, the cell trap having a storage space in which cells are stored in an inner side direction; And a side channel connected to the main channel for generating a flow in the storage space of the cell trap by the solution exiting through the side channel outlet, the side channel having an inlet and an outlet, ≪ / RTI >

Description

 TECHNICAL FIELD [0001] The present invention relates to a microfluidic channel device and a method of using the microfluidic channel device,

Disclosure relates generally to microfluidic channel devices, and more particularly to microfluidic channel devices capable of storing cells in and out of a storage space.

Herein, the background art relating to the present disclosure is provided, and these are not necessarily meant to be known arts.

There are a variety of techniques in which microfluidic channel devices are used in techniques to treat cells from a few micrometers to tens of micrometers in small volumes and as single cells. It is important to immobilize cells in a microfluidic channel device for a predetermined period of time and to observe cell growth and the like. However, as the scale of the microfluidic channel device is small, it is not easy to control the flow in the device and it is more difficult to control the cells that are moved by the flow. Despite these limitations, various attempts have been made to implement the aforementioned technique.

1 is a view showing a single cell separation and position fixation and maintenance apparatus disclosed in Korean Patent Laid-Open Publication No. 10-2014-0071400.

The single cell separation and position fixation apparatus includes a first layer 100 including a fluid channel 10 through which a fluid including cells flows, and a plurality of air channels 21, 22, 23, 24, 25 32, 33, 34, 35, 36, 37, 38 at the inner end of the air channel, and a second layer (200) Is formed. The positions of the cells can be maintained by using the vibrating portions 31, 32, 33, 34, 35, 36, 37, and 38. This method is effective in separating into single cells and stopping cell migration. However, there is a disadvantage that the vibration must be controlled by injecting air from the outside.

2 is a view showing a microfluidic device and a method of separating a target using the microfluidic device disclosed in Korean Patent Laid-Open No. 10-2011-0115478.

The microfluidic device according to the present invention separates the targets 6 by firstly filtering a plurality of non-targets 4 and plural types of targets 6 contained in the sample 2, Separate targets (6) are separated by secondary filtration according to size. The sample 2 is supplied from the sample container 60 to the pipe 34c. The coupling device (30) is composed of a sleeve (32). The sleeve 32 has a bore 32a which is connected to the bore 12a of the sleeve 12. A first male screw 32b and a second male screw 32c are respectively formed on the upper and lower ends of the sleeve 32, respectively. The second male screw 32c is fastened to the female screw 12e of the sleeve 12. A female screw 34b is formed on the lower surface of the inner surface of the bore 34a to fasten the first male screw 32b. And a pipe 34c is formed on the upper surface of the adapter 34. [ A first step (step 12b) is formed at the lower part of the outer surface of the sleeve 12. And a second end 12d that extends the diameter of the bore 12a is formed on the inner surface of the bore 12a. The edge of the mesh filter 14 is supported by the first end 12b of the sleeve 12. [ An introduction hole 52 is formed in the upper surface of the receiver 50 for introducing the sample 2 thereinto. The bottom of the space 54 is formed by an inclined surface 58 which is lowered toward the discharge hole 56 to guide the flow of the sample 2 flowing into the space 54 to the discharge hole 56. After removing the target (6) by pre-processing among a plurality of non-target (6) and plural types of targets (4) included in the sample (2), the target (4) obtained in the pre- . Therefore, the plurality of types of targets 4 can be efficiently filtered and separated according to their sizes, and can be very useful for separating and collecting cells from human blood or the like. However, since it is necessary to pass multiple filters in order to obtain a desired target 4, there is a problem that the target 4 may be subjected to stress.

This will be described later in the Specification for Implementation of the Invention.

Briefly, the present invention relates to the ability to move cells within a channel of a microfluidic channel device, store cells in a certain space, and retrieve stored cells from the device after a certain period of time. Also, there is a need for techniques that can fix cells in a certain space and change the surrounding solution. It is a necessary technique to observe the growth and the change of the cell by the solution around the cell in the culture of the cell, that is, the solution. And we need a technique to store the cells in the space for a certain period of time and retrieve them again. A function of screening is required to acquire only cells expressing a specific phenomenon.

SUMMARY OF THE INVENTION Herein, a general summary of the present disclosure is provided, which should not be construed as limiting the scope of the present disclosure. of its features).

According to one aspect of the present disclosure, there is provided a microfluidic channel device in which a solution containing cells flows in a predetermined direction, comprising: a main channel; 1. A cell trap provided in a main channel, the cell trap comprising: a cell trap having an inner side surface, an outer side surface and an inlet, the cell trap having a storage space in which cells are stored in an inner side direction; And a side channel connected to the main channel for generating a flow in the storage space of the cell trap by the solution exiting through the side channel outlet, the side channel having an inlet and an outlet, Device is provided.

This will be described later in the Specification for Implementation of the Invention.

Briefly, In the microfluidic channel device, the cells can be fixed in the cell trap for a certain period of time and the cells can be observed. In addition, cells can be immobilized in cell traps and the solution around the cells can be changed. Also, a microfluidic channel device capable of storing the cells in a cell trap for a certain period of time and then removing the cells again is provided.

1 is a view showing a single cell separation and position fixation and maintenance apparatus disclosed in Korean Patent Laid-Open No. 10-2014-0071400,
2 is a view showing a microfluidic device and a method of separating a target using the microfluidic device disclosed in Korean Patent Laid-Open No. 10-2011-0115478,
3 is a diagram illustrating an example of a microfluidic channel device according to the present disclosure,
4 is a diagram illustrating another example of a microfluidic channel device according to the present disclosure,
5 is a diagram illustrating still another example of a microfluidic channel device according to the present disclosure,
6 is a diagram detailing cell traps according to the present disclosure,
7 is a diagram detailing side channels, main channels and cell traps according to the present disclosure,
8 is a diagram illustrating an embodiment of a microfluidic channel device according to the present disclosure,
FIG. 9 is a view showing flows formed by side channels in a storage space of a cell trap according to the present disclosure; FIG.
10 is a diagram showing another example of a flow formed by a side channel in a storage space of a cell trap according to the present disclosure;
11 is a diagram showing an example of a method of using a microfluidic channel device according to the present disclosure;

The present disclosure will now be described in detail with reference to the accompanying drawings.

The present invention provides a microfluidic channel device capable of immobilizing a cell in a predetermined space for a predetermined time and at the same time changing a solution irrespective of time and amount and retrieving the cell after storing the cell.

3 is a diagram illustrating an example of a microfluidic channel device according to the present disclosure;

The microfluidic channel device includes a main channel (100), a cell trap (200), and a side channel (300). The main channel 100 includes an inlet 101 and an outlet 102 and a solution containing cells flows from the inlet 101 to the outlet 102. The cell trap 200 has an inner surface 203 and an outer surface 204 and has a storage space 201 in which cells are stored in the direction of the inner surface 203. The side channel 300 has an inlet 301 and an outlet 302 through which the solution flows from the inlet 301 to the outlet 302. The cell trap 200 is provided inside the main channel 100 and stores the cells contained in the solution flowing along the main channel 100 in the storage space 201. The side channel 300 is connected to the main channel 100 and the solution from the outlet 302 of the side channel 300 flows into the storage space 201 where the cells are stored. When a flow occurs in the storage space 201 of the cell trap 200, stored cells may escape from the storage space 201. The details will be described in detail with reference to FIG. The solution containing the cells flows from the inlet 101 of the main channel 100 to the outlet 102 of the main channel 100. The main channel 100 can be connected to the inlet 101 of the main channel 100 and the solution can be pushed into the main channel 100 using the pressure of the syringe, To the outlet 102 of the outlet. The syringe can then be connected to an external device that can adjust the pressure and speed to adjust the pressure and velocity of the solution. The cells are trapped in the storage space 201 by the cell trap 200 and stored in the storage space 201. The solution of the side channel 300 flows from the inlet 301 to the outlet 302 of the side channel 300 and the solution from the outlet 302 is caused to flow to the storage space 201 for storage The cells in the space 201 are taken out. The syringe is connected to the inlet 301 of the side channel 300 and the solution is pushed into the side channel 300 using the pressure of the syringe so that the side channel 300 at the inlet 301 of the side channel 300, The solution may flow to the outlet 302 of the reactor. At this time, the syringe can be connected to an external device capable of adjusting the pressure and the speed to adjust the pressure and the velocity of the solution. The external device may be used together in the side channel 300 and the main channel 100. The solution flowing in the main channel 100 contains cells, and the solution flowing in the side channel 300 does not include cells. The solution flowing through the main channel 100 and the side channel 300 uses a solution for storing cells since the cells are stored in the storage space 201 and the cells are taken out after culturing. It is desirable to use a solution that is compatible with the characteristics of the cells to protect the cells. For example, microalgae that grow in the ocean use a solution that matches the cell's characteristics when they enter normal fresh water because the cells are destroyed by osmotic pressure.

4 is a view showing another example of a microfluidic channel device according to the present disclosure.

The main channel (100) includes a convex portion (103). The convex portion 103 is convex than the peripheral main channel 100. The convex portion 103 is provided with a cell trap 200 and the convex portion 103 is provided with a side channel 300. The main channel 100 may include one or more cell traps 200. 4, the side channel 300 may be divided into a plurality of channels. 3 is substantially the same as the microfluidic channel device described in Fig.

5 is a diagram showing still another example of a microfluidic channel device according to the present disclosure.

5 (a) shows an example in which a plurality of cell traps 200 and a plurality of side channels 300 are provided in the convex portion 103. FIG. And an example in which each side channel 300 is provided in a plurality of cell traps 200. A plurality of cell traps 200 are provided so that cells not attached to the cell traps 200 at the entrance 101 side of the main channel 100 are caught by the other cell traps 200. The inlet 202 of the cell trap 200 is preferably positioned in a direction in which the solution flowing in the main channel 100 and the solution flowing out of the side channel 300 enter. 5B is an example in which the main channel 100 is divided into a plurality of branches and a plurality of cell traps 200 and a plurality of side channels 300 are provided in the main channel 100. FIG. 3 is substantially the same as the microfluidic channel device described in Fig.

6 is a diagram detailing cell traps according to the present disclosure;

The cell trap 200 includes at least one hole 600 penetrating the inner side surface 203 and the outer side surface 204. When the solution is filled in the storage space 201, the cells 500 can not enter, and the cells 500 that have entered the storage space 201 also come out of the solution. The width of the at least one hole 600 should be smaller than the diameter of the cell 500 so that the cell 500 can not pass through the at least one hole 600. That is, the size of the at least one hole 600 is preferably smaller than the size of the cell 500. The hole 600 includes an inlet 601 and an outlet 602. The inlet (601) may be smaller than the size of the outlet (602). For example, when the cell size is 8 to 15 μm, the size of the inlet 601 of the hole 600 formed in the inner side surface 203 is 7.8 μm and the size of the outlet 602 of the hole 600 formed in the outer side surface 204 ) Is 13 mu m. The cells 500 are stored in the storage space 201 and the solution is discharged from the storage space 201 through at least one hole 600. [ As a result, the cells 500 are stored in the storage space 201 and can not escape. Is substantially the same as the cell trap 200 described in Fig. 3, except as described in Fig.

7 is a diagram detailing side channels, main channels and cell traps according to the present disclosure; 7 (a) is a view showing a side channel, a main channel and a cell trap, and Fig. 7 (b) is a view showing a cross section taken along line A-A 'in Fig.

The side channel 300 generates a flow in the storage space 201 of the cell trap 200. The inlet 202 of the cell trap 200 is preferably in the direction 210 where the solution exits at the outlet 302 of the side channel 300. The solution from the outlet 302 of the side channel 300 generates a flow inside the storage space 201 of the cell trap 200. The solution from the outlet 302 of the side channel 300 moves to the storage space 201 where the cells are stored and the solution moves along the inner surface 203 to cause the storage space 201 to flow. As a result, the cells 500 in the storage space 201 of the cell trap 200 can come out of the storage space 201 together with the solution by the flow of the solution. The main channel 100 is provided with a convex portion 103 and includes a cell trap 200 provided inside the convex portion 103 and a side channel 300 through which cells trapped in the cell trap 200 can be taken out do. The cell trap 200 is formed to have a diameter of 1.5 times the width 120 of the main channel 100 and has a width 220 of the cell trap 200 so as to be 0.5 to 2 times the height 130 of the main channel 100 ) Is preferably formed. This is because the cell trap 200 must withstand the pressure of the solution. The width 340 of the side channel 300 is formed to be narrower than the width 120 of the main channel 100 so that a flow faster than the side channel 300 can be generated even if the same inlet 101, desirable. The speed of the solution exiting the side channel 300 or the main channel 100 can be determined by the width of the channel and the pressure at which the solution is injected. The closer the outlet 302 of the side channel 300 to the cell trap 200, the better. The side channel 300 may have an angle 350 between 0 and 90 degrees with the main channel 100 to effectively flow into the storage space 201 of the cell trap 200. The shortest distance 110 between the outer surface 204 of the cell trap 200 and the inner surface 104 of the main channel 100 is equal to or greater than the diameter of the cell. The cells exiting through the flow are directed toward the outer surface 204 of the cell trap 200 and the main Because it must pass between the inner side 104 of the channel 100.

For example, if the cell size is 8 to 15 μm, the diameter of the inner surface 203 of the cell trap 200 is 150 μm, and the diameter of the outer surface 204 of the cell trap 200 is 250 μm. The diameter of the convex portion 103 may be 400 mu m. The width 120 of the main channel 100 may be 100 占 퐉 and the width 340 of the side channel 300 may be 50 占 퐉. In the microfluidic channel device having such a size, the input flow rate of the main channel 100 can be applied in a range of 0.01 μL / min to 10 μL / min, and it is desirable to input a rate of 0.1 μL / min. The input flow rate of the side channel 300 can be 1 to 1000 times the speed of the main channel 100 and 1 to 500 μL / min can be applied. The outlet 302 of the side channel 300, Is preferably 10 [mu] L / min. It is preferable that the outlet 302 of the side channel 300 and the cell trap 200 are closer to each other and the distance is preferably 70 μm in the microfluidic channel device having the above size. The side channel 300 may have an angle 350 between 0 and 90 degrees with the main channel 100 to effectively flow into the storage space 201 of the cell trap 200, In the microfluidic channel device, the main channel 100 may have an angle of 45 degrees. Is substantially the same as the microfluidic channel device described in Fig. 3, except as described in Fig.

8 is a view showing an embodiment of a microfluidic channel device according to the present disclosure;

8A is a view showing cells 500 stored in the storage space 201 of the cell trap 200. FIG. The solution containing the cells 500 in the main channel 100 flows from the inlet 101 to the outlet 102 of the main channel 100. The cells 500 in the flowing solution enter the storage space 201 of the cell trap 200 and are caught in the storage space 201 of the cell trap 200. 8B shows a state in which the storage space 201 is empty after the cell 500 is taken out of the cell trap 200. FIG. A solution not containing the cells 500 is inserted into the side channels to generate a flow in the storage space 201 of the cell traps 200. And the cells 500 in the storage space 201 have escaped from the storage space 201 due to the occurrence of the flow.

9 is a view showing the flow formed by the side channel in the storage space of the cell trap according to the present disclosure.

9 (a) shows an example in which cells are drawn out by causing a flow in the clockwise direction 320 inside the cell trap 200. The solution 310 discharged from the outlet 302 of the side channel 300 is discharged from the storage space 201 of the cell trap 200 while the side channel 300 forms an angle of 45 degrees to 90 degrees with the main channel 100, So that the cells can escape from the storage space 201 as the flow occurs in the clockwise direction (320). 9 (b) shows an example in which the cells are drawn out in the counterclockwise direction 330 in the cell trap 200. The angle of the side channel 300 with respect to the main channel 100 is about 0 to 45 degrees and the angle between the side channel 300 and the main channel 100 is close to 0 degree, The solution 310 discharged from the outlet 302 of the cell trap 300 may flow out of the storage space 201 while forming a flow in the counterclockwise direction 330 in the storage space 201 of the cell trap 200 .

10 is a view showing another example of the flow formed by the side channel in the storage space of the cell trap according to the present disclosure;

The diameter of the inner surface 203 of the cell trap 200 is formed to be larger than the inner surface 203 of the cell trap 200 of FIG. The inner side surface 203 of the cell trap 200 is formed to extend outwardly from the inner side surface 203 of the cell trap 200 of FIG. 9, so that the side channel 300 can more easily hold the cells in the storage space 201 I can take it out. Is substantially the same as the cell trap 200 described in Fig. 3, except as described in Fig.

11 is a diagram showing an example of a method of using the microfluidic channel device according to the present disclosure.

In the microfluidic channel device using method, a solution 700 containing cells 500 flowing along the main channel 100 is injected. Thereafter, the cell 500 is stored in the storage space 201 of the cell trap 200 in the main channel 100. At this time, the solution 700 flowing in the main channel 100 can be replaced with another solution 701. For example, nutrient-depleted or de-nutrient solution 701 may be allowed to flow through the main channel 100 so that the cells 500 stored in the storage space 201 can be cultured. At this time, only the solution 701 escapes by the holes 600 in the cell trap 200, and the cells 500 are stored in the storage space 201. Thereafter, the cell 500 is extracted from the storage space 201 of the cell trap 200 using the side channel 300. At this time, the cell 500 can be taken out by causing the cell 500 to flow into the storage space 201 of the cell trap 200 using the side channel 300. For example, as shown in FIG. 9 (b), the solution 310 from the outlet 302 of the side channel 300 causes a flow in the counterclockwise direction 330 inside the cell trap 200. Further, it is possible to cause a flow as shown in Fig. 9 (a). Cells 500 that have passed through the storage space 201 may be stored at the outlet 102 of the main channel 100.

Various embodiments of the present disclosure will be described below.

(1) A microfluidic channel device in which a solution containing cells flows in a predetermined direction, comprising: a main channel; 1. A cell trap provided in a main channel, the cell trap comprising: a cell trap having an inner side surface, an outer side surface and an inlet, the cell trap having a storage space in which cells are stored in an inner side direction; And a side channel connected to the main channel for generating a flow in the storage space of the cell trap by the solution exiting through the side channel outlet, the side channel having an inlet and an outlet, Device.

(2) The outlet of the side channel is located such that the solution coming out of the side channel outlet is directed to the inlet of the cell trap.

(3) The microfluidic channel device according to any one of (1) to (3), wherein the main channel includes a convex portion that is convex than the surrounding portion, and a cell trap is provided in the convex portion.

(4) the entrance of the cell trap is located in the direction in which the solution exits at the outlet of the side channel.

(5) The microfluidic channel device according to (5), wherein the inlet of the cell trap is located in a direction in which the solution flowing in the main channel and the solution emerging from the side channel enter.

(6) The microfluidic channel device as claimed in claim 1, wherein the cell trap includes at least one hole penetrating an inner side surface and an outer side surface.

(7) A microfluidic channel device characterized in that the size of the hole is smaller than the cell.

(8) The microfluidic channel device according to (8), wherein the main channel has an inner surface, and the shortest distance between the outer surface of the cell trap and the inner surface of the main channel is not less than the cell size.

(9) the main channel has an inner surface, the main channel includes a convex portion more convex than the surroundings, the convex portion has a cell trap, and the inlet of the cell trap is formed by a solution flowing in the main channel and a solution And the cell trap includes at least one hole penetrating the inner side and the outer side, the size of the hole being smaller than that of the cell, the shortest distance between the outer side of the cell trap and the inner side of the main channel is larger than the cell size , The outlet of the side channel is located such that the solution from the side channel outlet is directed towards the inlet of the cell trap and the inlet of the cell trap is located in the direction of the solution exit at the outlet of the side channel.

(10) A method of using a microfluidic channel device, comprising: injecting a solution containing cells flowing along a main channel; Storing cells in a storage space of cell traps in the main channel; And withdrawing the cells from the cell trap storage space using the side channels.

(11) storing cells in a cell trap storage space in the main channel, wherein the solution of the main channel is replaced with another solution.

(12) withdrawing the cells from the cell trap storage space using the side channel, and flowing the cell trap storage space with the solution flowing through the side channel to withdraw the cells.

According to the present disclosure, there is provided a microfluidic channel device capable of immobilizing cells in a microfluidic channel device for a predetermined period of time in a cell trap and observing the cells.

The present disclosure also provides a microfluidic channel device capable of immobilizing cells in cell traps and simultaneously altering the solution around the cells.

According to the present disclosure, there is also provided a microfluidic channel device capable of storing cells in a cell trap for a predetermined period of time and then removing the cells again.

The present disclosure also provides a microfluidic channel device capable of storing and withdrawing cells using flow.

Also, according to the present disclosure, there is provided a microfluidic channel device capable of storing cells in a storage space without stress and extracting them from a storage space.

100: Main channel 101: Entrance of main channel
102: outlet of main channel 103: convex of main channel
110: Shortest distance 120: Width
200: Cell trap 201: Storage space of cell trap
202: entrance of cell trap 203: inner side of cell trap
204: outer surface of the cell trap 210: direction
300: side channel 301: entrance of the side channel
302: outlet of the side channel 310: solution
320: clockwise 330: counterclockwise
340: width 350: angle
500: cell 600: hole
601: entrance 602: exit

Claims (12)

In a microfluidic channel device in which a solution containing cells flows in a certain direction,
Main channel;
1. A cell trap provided in a main channel,
A cell trap having an inner side and an outer side and an inlet, the cell trap having a storage space in which cells are stored in an inner side direction; And,
A side channel having an inlet and an outlet,
A side channel connected to the main channel for generating a flow in the storage space of the cell trap by the solution exiting through the side channel outlet,
The outlet of the side channel is located such that the solution from the side channel outlet is directed to the inlet of the cell trap,
Wherein the solution from the side channel outlet causes the cells to exit the storage space. The method according to claim 1,
Wherein the outlet of the side channel is positioned such that the solution from the side channel outlet is directed toward the inlet of the cell trap. The method according to claim 1,
The main channel includes convex portions that are convex around the circumference,
Wherein a cell trap is provided on the convex portion. The method according to claim 1,
Wherein the inlet of the cell trap is located in the direction in which the solution exits at the outlet of the side channel. The method according to claim 1,
Wherein the inlet of the cell trap is located in a direction in which the solution flowing in the main channel and the solution coming out of the side channel enter. The method according to claim 1,
Wherein the cell trap includes at least one hole penetrating the inner side surface and the outer side surface. The method of claim 6,
And the size of the hole is smaller than that of the cell. The method according to claim 1,
The main channel has an inner surface,
Wherein the shortest distance between the outer surface of the cell trap and the inner surface of the main channel is at least the cell size. The method according to claim 1,
Wherein the main channel has an inner surface, the main channel includes a convex portion that is convex than the surrounding portion, a cell trap is provided on the convex portion,
The cell trap is located in the direction in which the solution flowing from the main channel and the solution coming out from the side channel enter. The cell trap includes at least one hole penetrating the inner and outer surfaces. The size of the hole is smaller than that of the cell. The shortest distance between the outer surface of the trap and the inner surface of the main channel is larger than the cell size,
Wherein the outlet of the side channel is located such that the solution from the side channel outlet is directed to the inlet of the cell trap and the inlet of the cell trap is located in the direction of solution exit at the outlet of the side channel. A method of using a microfluidic channel device,
Injecting a solution containing cells flowing along the main channel;
Storing cells in a storage space of cell traps in the main channel; And,
Withdrawing the cells from the storage space of the cell trap using the side channel,
Removing the cells from the storage space of the cell trap using side channels,
The outlet of the side channel is located such that the solution from the side channel outlet is directed to the inlet of the cell trap,
Wherein the solution from the side channel outlet causes the cells to exit the storage space. The method of claim 10,
Wherein cells are stored in a storage space of cell traps in the main channel
Wherein the solution of the main channel is replaced with another solution. The method of claim 10,
Withdrawing the cells from the storage space of the cell trap using the side channel;
Wherein a flow is made in the storage space of the cell trap with the solution flowing through the side channel to withdraw the cell.
KR1020160002583A 2016-01-08 2016-01-08 Microfluidic channel device and method for using the same KR101850852B1 (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005140790A (en) 2003-11-07 2005-06-02 Steag Microparts Gmbh Microstructure type separation apparatus and separating method for separating liquefied component from particle-containing liquid
JP2009109232A (en) * 2007-10-26 2009-05-21 Josho Gakuen Device having solid-liquid separation function, and its manufacturing method
JP2010279908A (en) * 2009-06-05 2010-12-16 Kazusa Dna Kenkyusho Three-dimensional sheath flow forming structure and method for collecting fine particles

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005140790A (en) 2003-11-07 2005-06-02 Steag Microparts Gmbh Microstructure type separation apparatus and separating method for separating liquefied component from particle-containing liquid
JP2009109232A (en) * 2007-10-26 2009-05-21 Josho Gakuen Device having solid-liquid separation function, and its manufacturing method
JP2010279908A (en) * 2009-06-05 2010-12-16 Kazusa Dna Kenkyusho Three-dimensional sheath flow forming structure and method for collecting fine particles

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