KR101850290B1 - Method for producing oriental medicine sauce for steamed meat and oriental medicine sauce for steamed meat produced by same method - Google Patents
Method for producing oriental medicine sauce for steamed meat and oriental medicine sauce for steamed meat produced by same method Download PDFInfo
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- KR101850290B1 KR101850290B1 KR1020160091743A KR20160091743A KR101850290B1 KR 101850290 B1 KR101850290 B1 KR 101850290B1 KR 1020160091743 A KR1020160091743 A KR 1020160091743A KR 20160091743 A KR20160091743 A KR 20160091743A KR 101850290 B1 KR101850290 B1 KR 101850290B1
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L23/00—Soups; Sauces; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/06—Meat products; Meat meal; Preparation or treatment thereof with gravy or sauce
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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Abstract
본 발명은 (a) 계피에 물과 효소를 첨가한 후 추출하여 계피 추출물을 제조하는 단계; (b) 정향에 물과 효소를 첨가한 후 추출하여 정향 추출물을 제조하는 단계; (c) 녹차 잎에 물과 효소를 첨가한 후 추출하여 녹차 추출물을 제조하는 단계; (d) 오미자에 물과 효소를 첨가한 후 추출하여 오미자 추출물을 제조하는 단계; (e) 상백피에 물과 효소를 첨가한 후 추출하여 상백피 추출물을 제조하는 단계; 및 (f) 고추 분말, 양파 다이스(dice), 칠리소스, 간장, 참기름, 양파분말, 마늘분말 및 물과 상기 (a)단계의 계피 추출물, 상기 (b)단계의 정향 추출물, 상기 (c)단계의 녹차 잎 추출물, 상기 (d)단계의 오미자 추출물 및 상기 (e)단계의 상백피 추출물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고기찜용 한방소스의 제조방법, 상기 방법으로 제조된 고기찜용 한방소스 및 상기 고기찜용 한방소스와 고기를 혼합한 후 재우고, 열을 가하여 제조하는 것을 특징으로 하는 고기찜의 제조방법에 관한 것이다.(A) preparing a cinnamon extract by adding water and an enzyme to cinnamon and extracting the cinnamon; (b) preparing a clove extract by adding water and an enzyme to the clove and extracting the clove; (c) preparing green tea extract by adding water and enzyme to the green tea leaves and extracting the green tea leaves; (d) adding water and enzyme to the omija and extracting it to prepare an omija extract; (e) adding water and enzyme to the bark, and extracting the bark bark extract; And (c) a cinnamon extract of step (a), a clove extract of step (b), and a mixture of the above-mentioned (c) Wherein the green tea leaf extract of step (d), the omija extract of step (d), and the corynebactor bark extract of step (e) are mixed. Wherein the sauce is prepared by mixing a sauce for one-time sauce, a sauce for sauce for meat and meat, putting on the sauce, and applying heat thereto.
Description
본 발명은 고추 분말, 양파 다이스(dice), 칠리소스, 간장, 참기름, 양파분말, 마늘분말, 물, 계피 추출물, 정향 추출물, 녹차 잎 추출물, 오미자 추출물 및 상백피 추출물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고기찜용 한방소스의 제조방법, 상기 방법으로 제조된 고기찜용 한방소스 및 상기 고기찜용 한방소스와 고기를 혼합한 후 재우고, 열을 가하여 제조하는 것을 특징으로 하는 고기찜의 제조방법에 관한 것이다.The present invention relates to a process for preparing a starch, comprising mixing the capsicum powder, onion dice, chili sauce, soy sauce, sesame oil, onion powder, garlic powder, water, cinnamon extract, clove extract, green tea leaf extract, Wherein the sauce is prepared by mixing the sauce and the sauce for the steamed meat prepared in accordance with the above method and the sauce for the steamed meat, putting the sauce into a sauce, .
현재, 돼지고기나 닭고기는 가장 보편적으로 널리 알려져 가공 조리되어 취식되고 있는 육류로서, 다양한 형태의 조리방법이 사용되고 있다. 특히, 이러한 고기는 각각의 부위에 따라 육질이 다르고 조리방법 또한 다양하게 이루어진다. 일반 음식점이나 가정에서 사용되고 있는 고기 조리방법으로는 냉장보관된 생고기를 불판을 이용하여 굽거나 또는 각종 양념을 가미하여 굽거나 찜 요리가 이루어지고, 특히, 각종 양념을 가미시킨 조리방법에서는 고기소스가 사용된다.At present, pork and chicken are the most widely known and widely used processed meats, and various types of cooking methods are used. Especially, these meats have different meat quality according to each region and various cooking methods are also made. In meat cooking methods used in ordinary restaurants or at home, the raw meat refrigerated is cooked using a hot plate or baked with various kinds of seasonings or steamed, and in particular, in a cooking method in which various seasonings are added, Is used.
통상적인 육류용 소스에 사용되는 양념재료들은 간장, 소금, 물엿, 설탕, 마늘, 양파, 생강, 참기름, 후추, 고추장 등 전통양념을 적정한 양의 식용수에 섞어서 사용하는 것이 보통이다. 이러한, 소스는 상기 언급한 재료에 국한되지 않고 보다 많은 종류의 양념을 첨가시켜 소스를 사용한다. 이러한 소스를 이용한 고기 숙성과정은 상기 소스를 이용하여 조리하고자 하는 고기를 일정시간 담그고 양념 맛이 이 고기에 베이도록 하면, 조리를 위한 고기의 숙성과정이 이루어진다.The seasoning ingredients used in ordinary meat sauces are usually prepared by mixing traditional sauces such as soy sauce, salt, syrup, sugar, garlic, onion, ginger, sesame oil, pepper and hot pepper to an appropriate amount of drinking water. Such a source is not limited to the above-mentioned materials and uses a sauce by adding more kinds of sauce. In the meat aging process using the sauce, the meat to be cooked is immersed for a predetermined time using the above-mentioned sauce, and a seasoning flavor is given to the meat, the meat is aged for cooking.
그러나 종래의 소스를 이용한 고기의 숙성과정에서는 소스가 고기에 베어 소스 양념에 의한 맛은 향상시킬 수 있었으나, 숙성과정에서 고기가 갖는 냄새(비린내)와 혼합된 각종 잡냄새 등이 제거되지 않아 미각을 떨어뜨리게 되는 문제점을 안고 있으며, 기존에 소스를 제조 시 사용하는 재료들이 제한적이어서 맛과 영양이 다양하지 않은 실정이다.However, in the process of aging meat using the conventional sauce, the sauce could improve the taste due to bare sauce seasoning in meat, but the smell (fishy smell) of meat and various odor mixed with the meat during aging could not be removed, And the materials used in manufacturing the sauce are limited, so that the taste and nutrition are not varied.
한국공개특허 제2005-0110401호에는 한방재료를 함유한 기능성 한방소스의 제조방법이 개시되어 있고, 한국공개특허 제2016-0063636호에는 참당귀 잎 추출물을 포함하는 한방소스의 제조방법이 개시되어 있으나, 본 발명의 고기찜용 한방소스의 제조방법과는 상이하다.Korean Patent Laid-Open Publication No. 2005-0110401 discloses a method for producing a functional herbal sauce containing a herbal material, and Korean Patent Laid-Open Publication No. 2016-0063636 discloses a method for producing a herbal sauce comprising a safflower leaf extract , Which is different from the method for producing a herbal source for frying meat according to the present invention.
본 발명은 상기와 같은 요구에 의해 안출된 것으로서, 본 발명의 목적은 항비만 효과가 우수하면서 고기찜용 소스 제조에 적합한 한방소재를 선정하고, 재료 종류 및 배합비를 최적화하여 고기찜용 한방소스를 제조함으로써, 항비만 효과와 더불어 저장안정성이 우수하고, 풍미 및 기호도가 우수한 고기찜용 한방소스의 제조방법을 제공하는 데 있다.SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and an object of the present invention is to provide a method of manufacturing a sauce for sauce by selecting a sauce material suitable for producing sauce for steamed meat, , An anti-obesity effect, a storage stability, and an excellent flavor and taste.
상기 과제를 해결하기 위해, 본 발명은 (a) 계피에 물과 효소를 첨가한 후 추출하여 계피 추출물을 제조하는 단계; (b) 정향에 물과 효소를 첨가한 후 추출하여 정향 추출물을 제조하는 단계; (c) 녹차 잎에 물과 효소를 첨가한 후 추출하여 녹차 추출물을 제조하는 단계; (d) 오미자에 물과 효소를 첨가한 후 추출하여 오미자 추출물을 제조하는 단계; (e) 상백피에 물과 효소를 첨가한 후 추출하여 상백피 추출물을 제조하는 단계; 및 (f) 고추 분말, 양파 다이스(dice), 칠리소스, 간장, 참기름, 양파분말, 마늘분말 및 물과 상기 (a)단계의 계피 추출물, 상기 (b)단계의 정향 추출물, 상기 (c)단계의 녹차 잎 추출물, 상기 (d)단계의 오미자 추출물 및 상기 (e)단계의 상백피 추출물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고기찜용 한방소스의 제조방법을 제공한다.In order to solve the above problems, the present invention provides a method for producing a cinnamon extract, comprising the steps of: (a) preparing a cinnamon extract by adding water and an enzyme to cinnamon; (b) preparing a clove extract by adding water and an enzyme to the clove and extracting the clove; (c) preparing green tea extract by adding water and enzyme to the green tea leaves and extracting the green tea leaves; (d) adding water and enzyme to the omija and extracting it to prepare an omija extract; (e) adding water and enzyme to the bark, and extracting the bark bark extract; And (c) a cinnamon extract of step (a), a clove extract of step (b), and a mixture of the above-mentioned (c) Wherein the green tea leaf extract of step (d), the omija extract of step (d), and the extract of bark extract of step (e) are mixed.
또한, 본 발명은 상기 방법으로 제조된 고기찜용 한방소스를 제공한다.In addition, the present invention provides a herbal sauce source prepared by the above method.
또한, 본 발명은 상기 고기찜용 한방소스와 고기를 혼합한 후 재우고, 열을 가하여 제조하는 것을 특징으로 하는 고기찜의 제조방법을 제공한다.Further, the present invention provides a method for manufacturing steamed meat, characterized in that the sauce for meat is mixed with meat, followed by putting and heat treatment.
본 발명은 다양한 한방소재 중에서 항비만 효과가 우수하면서 고기찜용 소스 제조에 적합한 한방소재를 선정한 후, 추출 수율 및 풍미가 우수한 조건으로 각각의 한방소재를 최적의 조건으로 추출하고, 상기 추출한 추출물과 부재료들을 적정량 혼합하여 고기찜용 한방소스를 제조함으로써, 고기의 육질 및 풍미를 증진시킬 수 있는 한방소스를 제공할 수 있다. 또한, 기존의 소스에 비해 항비만 효과와 더불어 저장안정성이 우수하여 소비자들이 더욱 선호하는 소스를 제공할 수 있다.The present invention relates to a process for preparing a herbal raw material suitable for producing sauce for meat, which is excellent in anti-obesity effect among various herbal materials, extracting each herbal raw material under optimal conditions under excellent conditions of extraction yield and flavor, Can be mixed with an appropriate amount to produce a herbal sauce for steamed meat, thereby providing a herbal sauce capable of improving meat quality and flavor of meat. In addition, it has superior storage stability as well as anti-obesity effect compared with the existing source, so that it can provide more preferable source for consumers.
도 1은 각각의 처리군에 따른 실험종료시점의 몸무게에서 실험시작시점의 몸무게를 빼서 몸무게 변화를 비교한 그래프이다.
N: 정상식이군, Veh: 고지방식이군FIG. 1 is a graph comparing body weight changes by subtracting the weight at the start of the experiment from the weight at the end of the experiment according to each treatment group.
N: That's normal, Veh.
본 발명의 목적을 달성하기 위하여, 본 발명은 In order to achieve the object of the present invention,
(a) 계피에 물과 효소를 첨가한 후 추출하여 계피 추출물을 제조하는 단계;(a) preparing a cinnamon extract by adding water and an enzyme to cinnamon and then extracting it;
(b) 정향에 물과 효소를 첨가한 후 추출하여 정향 추출물을 제조하는 단계;(b) preparing a clove extract by adding water and an enzyme to the clove and extracting the clove;
(c) 녹차 잎에 물과 효소를 첨가한 후 추출하여 녹차 추출물을 제조하는 단계;(c) preparing green tea extract by adding water and enzyme to the green tea leaves and extracting the green tea leaves;
(d) 오미자에 물과 효소를 첨가한 후 추출하여 오미자 추출물을 제조하는 단계;(d) adding water and enzyme to the omija and extracting it to prepare an omija extract;
(e) 상백피에 물과 효소를 첨가한 후 추출하여 상백피 추출물을 제조하는 단계; 및 (e) adding water and enzyme to the bark, and extracting the bark bark extract; And
(f) 고추 분말, 양파 다이스(dice), 칠리소스, 간장, 참기름, 양파분말, 마늘분말 및 물과 상기 (a)단계의 계피 추출물, 상기 (b)단계의 정향 추출물, 상기 (c)단계의 녹차 잎 추출물, 상기 (d)단계의 오미자 추출물 및 상기 (e)단계의 상백피 추출물을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 고기찜용 한방소스의 제조방법을 제공한다.(a), the clove extract of step (b), the clove extract of step (b), the step (c) of step (c) , The green tea leaf extract of step (d), the omija extract of step (d), and the extract of Mulberry bark extract of step (e).
본 발명의 고기찜용 한방소스의 제조방법에서, 상기 효소는 α-아밀라아제(α-amylase) 또는 글루코아밀라아제(glucoamylase)일 수 있으나, 이에 제한되지 않는다.In the method for producing a herbal source for frying meat according to the present invention, the enzyme may be, but is not limited to, alpha -amylase or glucoamylase.
또한, 본 발명의 고기찜용 한방소스의 제조방법에서, 상기 고기는 닭고기, 오리고기, 돼지고기, 소고기 또는 말고기일 수 있으며, 더욱 바람직하게는 닭고기일 수 있으나, 이에 제한되지 않는다.In addition, in the method for producing a herbal sauce for meat frying according to the present invention, the meat may be chicken meat, duck meat, pork meat, beef meat or malted meat, more preferably chicken meat, but is not limited thereto.
또한, 본 발명의 고기찜용 한방소스의 제조방법에서, 상기 (f)단계의 혼합은 바람직하게는 고추 분말 28~36 g, 양파 다이스(dice) 28~36 g, 칠리소스 16~24 g, 간장 8~12 g, 참기름 5~7 g, 양파분말 0.8~1.2 g, 마늘분말 2.5~3.5 g, 물 12~18 g, 계피 추출물 0.1~0.3 g, 정향 추출물 1.4~2.0 g, 녹차 잎 추출물 0.6~1.0 g, 오미자 추출물 0.1~0.3 g 및 상백피 추출물 0.05~0.15 g을 혼합할 수 있으며, 더욱 바람직하게는 고추 분말 32 g, 양파 다이스(dice) 32 g, 칠리소스 20 g, 간장 10 g, 참기름 6 g, 양파분말 1 g, 마늘분말 3 g, 물 15 g, 계피 추출물 0.2 g, 정향 추출물 1.7 g, 녹차 잎 추출물 0.8 g, 오미자 추출물 0.2 g 및 상백피 추출물 0.1 g을 혼합할 수 있다. 상기와 같은 비율로 혼합하여 한방소스를 제조하는 것이, 저장안정성이 우수하고, 소비자들에게 탁월한 맛과 풍미를 제공하여 소비자들의 기호를 충족시킬 수 있다. 또한, 고기 특유의 누린내는 제거하고 육질을 부드럽게 하여 식감이 우수한 고기찜을 제조할 수 있는 이점이 있다.In the method for producing a sauce for meat frying in accordance with the present invention, the mixing in step (f) is preferably 28 to 36 g of pepper powder, 28 to 36 g of onion dice, 16 to 24 g of chili sauce, 8 ~ 12g, sesame oil 5 ~ 7g, onion powder 0.8 ~ 1.2g, garlic powder 2.5 ~ 3.5g, water 12 ~ 18g, cinnamon extract 0.1 ~ 0.3g, clove extract 1.4 ~ 2.0g, green tea leaf extract 0.6 ~ , More preferably, 32 g of pepper powder, 32 g of onion dice, 20 g of chili sauce, 10 g of soy sauce, 10 g of sesame oil, g, 1 g of onion powder, 3 g of garlic powder, 15 g of water, 0.2 g of cinnamon extract, 1.7 g of clove extract, 0.8 g of green tea leaf extract, 0.2 g of Omija extract and 0.1 g of the extract of Echinacea. It is possible to prepare a one-sided sauce by mixing in the same ratio as above to provide excellent taste and flavor to consumers and satisfy customers' taste because of excellent storage stability. In addition, there is an advantage that the meat-puddle-shaped noodles can be removed and the meat quality can be softened to produce a meat-steamed food having excellent texture.
본 발명의 고기찜용 한방소스의 제조방법은, 보다 구체적으로는The method for producing a one-sided sauce for steamed meat according to the present invention, more specifically,
(a) 계피에 물을 3.5~4.5배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 6~8시간 동안 추출하여 14~14.6 brix의 계피 추출물을 제조하는 단계;(a) adding water to the cinnamon at a rate of 3.5 to 4.5 times (v / w), adding the enzyme, and extracting at 80 to 100 ° C for 6 to 8 hours to prepare a cinnamon extract of 14 to 14.6 brix;
(b) 정향에 물을 2.7~3.3배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 6~7시간 동안 추출하여 16.4~17.0 brix의 정향 추출물을 제조하는 단계;(b) adding water to the clove at 2.7 to 3.3 times (v / w), adding the enzyme, and extracting at 80 to 100 ° C for 6 to 7 hours to prepare a clove extract of 16.4 to 17.0 brix;
(c) 녹차 잎에 물을 15~19배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 7~8시간 동안 추출하여 23.0~23.8 brix의 녹차 추출물을 제조하는 단계;(c) adding 15-19 times (v / w) water to the green tea leaves, adding the enzyme to the mixture, and extracting the mixture at 80-100 ° C for 7-8 hours to prepare a green tea extract of 23.0-23.8 brix;
(d) 오미자에 물을 7.5~8.5배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 5.5~6.5시간 동안 추출하여 19.0~19.8 brix의 오미자 추출물을 제조하는 단계;(d) adding 7.5 ~ 8.5 times (v / w) of water to the Schizosaccharomyces japonica, adding the enzyme to the mixture, and extracting the mixture at 80 ~ 100 ° C for 5.5 ~ 6.5 hours to prepare an omia extract of 19.0 ~ 19.8 brix;
(e) 상백피에 물을 10~14배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 7.5~8.5시간 동안 추출하여 12.4~13.0 brix의 상백피 추출물을 제조하는 단계; 및 (e) adding 10 to 14 times (v / w) water to the bovine alfalfa, adding the enzyme, and extracting it at 80 to 100 ° C for 7.5 to 8.5 hours to prepare a bovine manganic extract of 12.4 to 13.0 brix; And
(f) 고추 분말 28~36 g, 양파 다이스(dice) 28~36 g, 칠리소스 16~24 g, 간장 8~12 g, 참기름 5~7 g, 양파분말 0.8~1.2 g, 마늘분말 2.5~3.5 g 및 물 12~18 g과 상기 (a)단계의 계피 추출물 0.1~0.3 g, 상기 (b)단계의 정향 추출물 1.4~2.0 g, 상기 (c)단계의 녹차 잎 추출물 0.6~1.0 g, 상기 (d)단계의 오미자 추출물 0.1~0.3 g 및 상기 (e)단계의 상백피 추출물 0.05~0.15 g을 혼합하는 단계를 포함할 수 있으며,(f) 28 to 36 g of red pepper powder, 28 to 36 g of onion dice, 16 to 24 g of chili sauce, 8 to 12 g of soy sauce, 5 to 7 g of sesame oil, 0.8 to 1.2 g of onion powder, (A), 0.1 to 0.3 g of the cinnamon extract of step (b), 1.4 to 2.0 g of the cinnabar extract of step (b), 0.6 to 1.0 g of the green tea leaf extract of step (c) 0.1 to 0.3 g of the extract of Omija of step (d) and 0.05 to 0.15 g of the extract of the herb extract of step (e)
더욱 구체적으로는More specifically,
(a) 계피에 물을 4배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 7시간 동안 추출하여 14.3 brix의 계피 추출물을 제조하는 단계;(a) adding 4 times (v / w) water to cinnamon, adding enzyme, and extracting it at 80 to 100 ° C for 7 hours to prepare a cinnamon extract of 14.3 brix;
(b) 정향에 물을 3배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 6.5시간 동안 추출하여 16.7 brix의 정향 추출물을 제조하는 단계;(b) adding 3 times (v / w) of water to the clove, adding the enzyme, and extracting it at 80 to 100 ° C for 6.5 hours to prepare a clove extract of 16.7 brix;
(c) 녹차 잎에 물을 17배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 7.5시간 동안 추출하여 23.4 brix의 녹차 추출물을 제조하는 단계;(c) adding 17 times (v / w) water to the green tea leaves, adding the enzyme, and extracting it at 80-100 ° C for 7.5 hours to prepare a green tea extract of 23.4 brix;
(d) 오미자에 물을 8배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 6시간 동안 추출하여 19.4 brix의 오미자 추출물을 제조하는 단계;(d) adding 8 times (v / w) water to the Schizosaccharomyces japonica, adding the enzyme, and extracting it at 80 to 100 ° C for 6 hours to prepare a 19.4 brix Omiza extract;
(e) 상백피에 물을 12배량(v/w) 첨가한 후 효소를 첨가하여 80~100℃에서 8시간 동안 추출하여 12.7 brix의 상백피 추출물을 제조하는 단계; 및 (e) adding 12 times (v / w) water to the bovine alfalfa, adding the enzyme, and extracting it at 80 to 100 ° C for 8 hours to produce a bovine marijuana extract of 12.7 brix; And
(f) 고추 분말 32 g, 양파 다이스(dice) 32 g, 칠리소스 20 g, 간장 10 g, 참기름 6 g, 양파분말 1 g, 마늘분말 3 g 및 물 15 g과 상기 (a)단계의 계피 추출물 0.2 g, 상기 (b)단계의 정향 추출물 1.7 g, 상기 (c)단계의 녹차 잎 추출물 0.8 g, 상기 (d)단계의 오미자 추출물 0.2 g 및 상기 (e)단계의 상백피 추출물 0.1 g을 혼합하는 단계를 포함할 수 있다.(f) 32 g of red pepper powder, 32 g of onion dice, 20 g of chili sauce, 10 g of soy sauce, 6 g of sesame oil, 1 g of onion powder, 3 g of garlic powder and 15 g of water, 0.2 g of the extract, 1.7 g of the cinnabar extract of the step (b), 0.8 g of the green tea leaf extract of the step (c), 0.2 g of the Omija extract of the step (d) .
본 발명의 고기찜용 한방소스의 제조방법에서, 상기 (a) 내지 (e)단계의 조건으로 계피, 정향, 녹차 잎, 오미자 및 상백피를 추출하는 것이 재료 특유의 이취나 풋내는 나지 않으면서 추출 수율이 우수한 이점이 있다.In the method for producing a sauce for meat frying according to the present invention, the extraction of cinnamon, clove, green tea leaves, omija and morifolium from the conditions of the above (a) to (e) This is an excellent advantage.
본 발명은 또한, 상기 방법으로 제조된 고기찜용 한방소스를 제공한다.The present invention also provides a herbal sauce source prepared by the above method.
본 발명은 또한, 상기 고기찜용 한방소스와 고기를 혼합한 후 재우고, 열을 가하여 제조하는 것을 특징으로 하는 고기찜의 제조방법을 제공한다.The present invention also provides a method for producing steamed meat, characterized in that the sauce for meat and steam is mixed with meat, followed by putting and heating.
본 발명의 고기찜의 제조방법은 보다 구체적으로는, 고기찜용 한방소스 100~140 g과 고기 60~80 g을 혼합한 후 1~7℃에서 15~25분 동안 재우고 120~130℃에서 6~8분 동안 열을 가하여 제조할 수 있으며, 더욱 구체적으로는, 고기찜용 한방소스 122 g과 고기 70 g을 혼합한 후 3℃에서 20분 동안 재우고 125℃에서 7분 동안 열을 가하여 제조할 수 있다.
More specifically, the method for producing steamed meat according to the present invention comprises mixing 100 to 140 g of a sauce for cooking meat with 60 to 80 g of meat, putting it at 1 to 7 ° C for 15 to 25 minutes, More specifically, mixing 122 g of the herb sauce for meat-boiling with 70 g of meat, putting it at 3 캜 for 20 minutes, and heating at 125 캜 for 7 minutes .
이하, 본 발명의 실시예를 들어 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.
Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.
제조예Manufacturing example 1: 고기찜용 한방소스 제조 1: Manufacture of herbal sauce for meat
(a) 계피에 정제수를 4배량(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 7시간 동안 추출하여 14.3 brix의 계피 추출물을 제조하였다.(a) Four times (v / w) of purified water was added to the cinnamon, and the enzyme (Novozymes, α-amylase) was added and extracted at 80 to 100 ° C. for 7 hours to prepare a cinnamon extract of 14.3 brix.
(b) 정향에 정제수를 3배량(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 6.5시간 동안 추출하여 16.7 brix의 정향 추출물을 제조하였다.(b) To the clove, 3 times (v / w) of purified water was added and then the enzyme (Novozymes, α-amylase) was added and extracted at 80 to 100 ° C. for 6.5 hours to prepare a clove extract of 16.7 brix.
(c) 녹차 잎에 정제수를 17배량(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 7.5시간 동안 추출하여 23.4 brix의 녹차 추출물을 제조하였다.(c) Green tea leaves were added 17 times (v / w) of purified water, followed by addition of an enzyme (Novozymes, α-amylase) and extraction at 80 to 100 ° C. for 7.5 hours to prepare 23.4 brix of green tea extract.
(d) 오미자에 정제수를 8배(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 6시간 동안 추출하여 19.4 brix의 오미자 추출물을 제조하였다.(d) After adding 8 times (v / w) of purified water to Omija, the enzyme (Novozymes, α-amylase) was added and extracted at 80 to 100 ° C for 6 hours to prepare a 19.4 brix Omiza extract.
(e) 상백피에 정제수를 12배(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 8시간 동안 추출하여 12.7 brix의 상백피 추출물을 제조하였다.(e) The purified water was added 12 times (v / w) to the bovine alfalfa, and then the enzyme (novoszyme, α-amylase) was added and extracted at 80 to 100 ° C. for 8 hours to produce a 12.7 brix extract of bovine manganic acid.
(f) 청고추 분말 16 g, 홍고추 분말 16 g, 양파 다이스(dice) 32 g, 칠리소스 20 g, 간장 10 g, 참기름 6 g, 양파분말 1 g, 마늘분말 3 g 및 정제수 15 g과 상기 (a)단계의 계피 추출물 0.2 g, 상기 (b)단계의 정향 추출물 1.7 g, 상기 (c)단계의 녹차 잎 추출물 0.8 g, 상기 (d)단계의 오미자 추출물 0.2 g 및 상기 (e)단계의 상백피 추출물 0.1 g을 혼합하였다.
(f) 16 g of blue pepper powder, 16 g of red pepper powder, 32 g of onion dice, 20 g of chili sauce, 10 g of soy sauce, 6 g of sesame oil, 1 g of onion powder, 3 g of garlic powder and 15 g of purified water, 0.2 g of the cinnamon extract of step (a), 1.7 g of the cinnabar extract of step (b), 0.8 g of the green tea leaf extract of step (c), 0.2 g of the omija extract of step (d) 0.1 g of the extract of Miscanthus sinensis was mixed.
제조예Manufacturing example 2: 2: 닭고기찜Steamed chicken 제조 Produce
닭가슴살 70 g에 상기 제조예 1의 제조한 한방소스를 혼합한 후 3℃ 냉장고에서 20분 동안 재웠다. 상기 재운 닭가슴살을 125℃에서 7분 동안 조리하였다.
The herbal sauce prepared in Preparation Example 1 was mixed with 70 g of chicken breast, and the mixture was allowed to stand in a refrigerator at 3 DEG C for 20 minutes. The luck chicken breast was cooked at 125 DEG C for 7 minutes.
실시예Example 1: 췌장 리파아제 억제 실험 1: Pancreatic lipase inhibition experiment
식이로 섭취된 지방은 대략 90% 정도가 중성지방들(Triglycerids; TGs)로 이루어져 있으며, 식이 지방의 분해와 흡수는 장내 낮은 pH에 의한 물리화학적인 지방의 변화와 아울러 여러 가지 효소작용이 동반되는 매우 복잡한 경로를 거쳐 진행이 된다. 이 중 췌장 리파아제는 지방의 물질대사에 관여하는 효소로 식이 지방 50~70% 가량을 지방산(Fatty acid; FA)과 모노글리세라이드(monoglycerides; MGs)로 가수분해하여 지방의 소화를 진행하고 장내 상피세포가 분해 산물을 흡수하도록 돕는다. 즉, FA와 MGs는 담즙산, 콜레스테롤, 리소포스파티드산(lysophosphatidic acid)과 미셀(micelles)을 형성하여 장세포 내로 흡수되어 들어오고 이를 통해 TGs가 재합성이 되고 이는 지방세포에 주요한 에너지원으로서 저장이 된다. 이처럼 지방은 췌장 리파아제에 의해 분해되어 흡수되기 때문에 췌장 리파아제 효소를 저해할 경우 혈중의 지방흡수를 저하시킬 수 있으며, 만약 췌장 리파아제가 부족하면 중성지방은 흡수되지 않고 대변으로 나오게 된다.Approximately 90% of the dietary fat is composed of triglycerides (TGs), and the degradation and absorption of dietary fat is accompanied by changes in physiochemical fat due to low pH in the intestines as well as various enzymatic actions It proceeds through a very complex path. Among them, pancreatic lipase is an enzyme involved in the metabolism of fat, and 50 to 70% of dietary fat is hydrolyzed with fatty acid (FA) and monoglycerides (MGs) to promote digestion of fat, Helps cells absorb degradation products. In other words, FA and MGs form cholesterol, cholesterol, lysophosphatidic acid and micelles, and are absorbed into intestinal cells, and through this, TGs are re-synthesized, which is stored as a main energy source in adipocytes . In this way, fat is absorbed by pancreatic lipase and is absorbed. Therefore, inhibition of pancreatic lipase enzyme may lower blood lipid absorption. If pancreatic lipase is insufficient, neutral fat is not absorbed and comes out in the feces.
본 실험에서는 26가지 다양한 약재의 췌장 리파아제 억제 실험을 실시하였다. 양성 대조군인 Orlistat는 식이 지방의 분변 내 배출을 통하여 체지방 축적 억제효과를 발휘하는 대표 약물로, 췌장 리파아제 억제실험에서 72.6%의 억제율을 나타내었고, IC50이 219 ng/mL로 우수한 효과를 발휘하였다. In this experiment, 26 pancreatic lipase inhibition experiments were performed on various kinds of medicines. Orlistat, a positive control, showed a suppressive effect of 72.6% in inhibition of pancreatic lipase and 219 ng / mL of IC 50, which is a representative drug for suppressing accumulation of body fat through fecal excretion of dietary fat .
또한, 28가지 다양한 약재의 췌장 리파아제 억제실험을 실시한 결과, 텍사, 황금, 인삼, 오미자, 영지, 정향, 치자, 다엽, 계피, 감초, 황련 및 상백피가 우수한 활성을 보였으나, 텍사의 경우 한약재는 가능하나 식품소재로 불가능하므로, 텍사는 제외한 11종의 약재를 이용하여 항비만 효과 추가 실험을 진행하였다.In addition, the pancreatic lipase inhibition test of 28 different medicines showed excellent activity of Tex, gold, ginseng, omija, ganoderma, clove, gardenia, multi leaf, cinnamon, licorice, However, since it is not possible to use it as a food material, TEXA conducted 11 additional kinds of medicines except for the anti-obesity effect.
실시예Example 2: 2: InIn vivovivo 지방 흡수 Fat absorption 억제능Inhibition 평가 evaluation
1. 실험방법1. Experimental Method
실시예 1에서 효과를 보인 약재 중 11종을 선별하여 췌장 리파아제 억제 동물 실험을 실시하였다.Eleven species of the medicinal substances effective in Example 1 were selected and tested for inhibiting pancreatic lipase.
6주의 웅성 ICR 마우스(하나바이오, 서울)를 구매하여, 물과 고형사료(항생제 무첨가, 삼양사)를 충분히 공급하며 1주간 실험실 환경에 적응시킨 후 실험에 사용하였다. 정상군은 일반사료를 급여하고, 고지방식이군과 약재 투여군은 Diet사에서 구입한 60% 고지방식이(HFD; Diet 12492, Research Diets, Inc., New Brunswick, NJ, USA)를 매일 새롭게 공급하였다(이는 사료의 산패를 방지하기 위함).A 6-week-old male ICR mouse (Hanabio, Seoul) was purchased and adapted to the laboratory environment for one week with sufficient supply of water and solid feed (no antibiotics, Samyang). (HFD; Diet 12492, Research Diets, Inc., New Brunswick, NJ, USA), purchased from Diet, were fed fresh daily (In order to prevent rancidity of the feed).
모든 마우스는 일정한 시간에 1회/1일 체중과 식이섭취량을 측정하였다. 고지방 식이군 중 대조군(Vehicle)은 증류수를, Orlistat는 매일 1회 60 mg/kg/day, 나머지 약재 투여군은 각각의 약재를 매일 1회 200 mg/kg/day 농도로 존대(Zonde)를 이용하여 시료를 경구투여 하였다.All mice were tested for body weight and food intake once / day at a given time. In the high fat diet group, the control group (Vehicle) was treated with distilled water, Orlistat was treated once daily with 60 mg / kg / day, and the rest group was treated with 200 mg / kg / Samples were orally administered.
일주일 약물투여 후 마우스의 심장에서 채혈한 혈액을 3,000 rpm으로 원심분리하여 혈청을 얻었으며, 마우스 혈청의 TG(triglyceride)는 TG assay kit(Wako Pure Chemical Industries, Ltd.)의 프로토콜에 따라 측정하였다.
TG (triglyceride) of mouse serum was measured according to the protocol of TG assay kit (Wako Pure Chemical Industries, Ltd.). The blood was collected by centrifugation at 3,000 rpm.
2. 실험결과2. Experimental results
(1) 체중변화(1) weight change
동물 실험을 시작하기 전 각 군은 임의적으로 배정하였으며, 각 군의 초기 몸무게는 유의성이 없음을 확인하였다. 또한, 도 1에 기재된 바와 같이, 실험종료시점의 몸무게에서 실험시작시점의 몸무게를 빼서 몸무게 변화를 살펴본 결과, 60% 고지방 식이만을 먹인 대조군(Veh)에 비해 Orlistat, 오미자, 정향, 영지 처리군에서 체중이 감소한 것을 확인할 수 있었고(*: p<0.05, **: p<0.01, ***: p<0.001), 또한, 육계(계피) 및 상백피도 유의적으로 체중이 감소한 것을 확인할 수 있었다(**: p<0.01, ***: p<0.001). 특히 Orlistat를 먹인 군은 초기무게보다 더 살이 빠지는 결과를 나타내었다. 반면, 다엽과 치자는 유의적으로 체중이 증가함을 알 수 있었다(#: p<0.05, ###: p<0.001).
Before starting the animal experiment, each group was randomly assigned, and initial weight of each group was not significant. In addition, as shown in FIG. 1, the body weight at the end of the experiment was subtracted from the body weight at the end of the experiment. As a result, the Orlistat, Omija, ( P <0.05, **: p <0.01, ***: p <0.001), and broiler weight (cinnamon) and cornucopia also significantly decreased in body weight **: p < 0.01, ***: p < 0.001). In particular, the group fed Orlistat showed more weight loss than the initial weight. On the other hand, it was found that the polyploidy and the gardenia significantly increased in body weight (#: p <0.05, ###: p <0.001).
(2) 혈중 중성지방(TG) 함량 측정(2) Measurement of blood triglyceride (TG) content
혈청을 분리하여 중성지방(TG)을 분석해 본 결과, 60% 고지방 식이만을 먹인 대조군에 비해서 치자를 제외한 모든 군의 중성지방이 감소함을 보여주었다. 특히, 인삼, 계피, 황련 및 치자를 제외한 약재 투여군에서는 모두 유의적인 감소 효과를 보여주었다(*: p<0.05, **: p<0.01, ***: p<0.001).Serum triglyceride (TG) analysis showed that the triglyceride levels of all groups except for the control group with the 60% high fat diet were decreased. ( P <0.05, **: p <0.01, ***: p <0.001), respectively, in the group treated with medicines except ginseng, cinnamon,
실시예Example 3: 약재별 추출 조건 확립 3: Establishment of extraction condition for each medicine
(1) 소재별 추출 조건 실험(1) Extraction condition experiment by material
일반적인 한방소재의 경우, 열수 추출의 방법이 보편적이며 대중적으로 사용되고 있다. 이는 기술적인 어려움이 없고, 가장 일반적인 대중화된 방법이라고 볼 수 있다. 근래에 들어 에탄올 등의 용매를 사용하여 추출하는 방법을 적용하기도 하지만, 이것은 에탄올 회수 장치가 있어야 가능하며, 일반적인 식품에 적용하기에는 불용성 소재가 함께 추출되기 때문에 적용의 한계가 있다. 따라서, 본 발명에서는 수용성 추출물의 제조를 위해, 일반적인 열수 추출법과 함께 분해효소를 사용하였다. 이에, 실시예 1 및 2의 결과, 항비만 효과가 있는 한방소재 중 가격이 저렴하여 경제적이면서 고기찜용 한방소스 제조에 적합한 한방소재(계피, 정향, 녹차잎, 오미자, 상백피)의 추출 조건을 설정하였다.In the case of general oriental materials, the method of hot water extraction is universal and widely used. This is not a technical difficulty, but it is the most common popularized method. In recent years, a method of extracting with a solvent such as ethanol has been applied. However, it is possible to use an ethanol recovery device and there is a limit to the application because insoluble materials are extracted together for general food. Therefore, in the present invention, a decomposition enzyme was used together with a general hot water extraction method for producing a water-soluble extract. Thus, as a result of Examples 1 and 2, the extraction conditions of the herbal materials (cinnamon, clove, green tea leaf, omija, and mantis bark) suitable for manufacturing herbal sauce for economical and inexpensive meats are established Respectively.
첫 번째로, 한방소재 대비 정제수의 배수를 달리하여 추출한 후, 고형분의 함량을 측정하여, 적정 정제수 첨가량을 결정하였다. 두 번째로, 한방소재와 정제수의 비율이 결정되고 난 후에, 동일한 온도에서 추출시간을 달리하여 추출한 후, 가장 수율이 높은 추출시간을 최적의 추출시간으로 설정하였다. 세 번째로, 정제수 첨가량과 추출시간이 확립되면, 추출의 온도를 단계별로 나누어 역시 최적의 추출조건을 확인하였다. 즉, 한방소재와 정제수의 비율을 먼저 확립하고, 이 후 동일온도 하에서의 추출 시간의 확립, 그리고 추출온도의 다양한 적용을 통해 한방 소재별 최적의 추출공정을 확립하였다. 네 번째로, 확립된 열수 추출의 조건 하에서 추출된 수율과 분해효소를 사용하여 추출한 수율과 비교하여 분해효소 첨가 유무를 확립하였다.
Firstly, the water content of the purified water was measured by varying the drainage amount of the purified water compared to the one - sided material, and the amount of the solid content was determined to determine the amount of the appropriate amount of purified water. Secondly, after the ratio of the raw material and the purified water was determined, the extraction time was set at the same temperature and then the optimum extraction time was set as the extraction time with the highest yield. Third, when the amount of purified water and the extraction time were established, the optimum extraction conditions were also confirmed by dividing the temperature of the extraction step by step. That is, firstly, the ratio of the raw material to the purified water was established first, then the extraction time was set at the same temperature, and the optimum extraction process for each oriental material was established through various application of the extraction temperature. Fourth, the addition of degradation enzymes was established by comparing the yields obtained under the conditions of established hot water extraction and the yields extracted using the degrading enzyme.
(2) 실험결과(2) Experimental results
추출 조건에 따른 실험 데이터는 자료가 너무 방대하여 명세서 내용에는 기재하지 않았지만, 계피의 경우, 계피에 정제수를 4배량(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 7시간 동안 추출하였고, 정향의 경우, 정향에 정제수를 3배량(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 6.5시간 동안 추출하였고, 녹차의 경우, 녹차 잎에 정제수를 17배량(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 7.5시간 동안 추출하였고, 오미자의 경우, 오미자에 정제수를 8배(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 6시간 동안 추출하였고, 상백피의 경우, 상백피에 정제수를 12배(v/w) 첨가한 후 효소(노보자임, α-amylase)를 첨가하여 80~100℃에서 8시간 동안 추출하는 것을 최적 추출조건으로 확립하였다.
In the case of cinnamon, 4 times (v / w) of purified water was added to cinnamon, and enzyme (α-amylase) was added to prepare 80 ~ (V / w) of purified water was added to the clove in the case of the clove, and then the enzyme (Novozymes,? -Amylase) was added and the mixture was extracted at 80 to 100 ° C for 6.5 hours. In the case of green tea, purified water was added 17 times (v / w) to the green tea leaves, followed by addition of enzyme (novozymes, α-amylase) and extraction at 80 to 100 ° C. for 7.5 hours. In case of omija, (V / w) was added, and the enzyme (novosime, α-amylase) was added and the mixture was extracted at 80 to 100 ° C. for 6 hours. (Nobozyme, α-amylase) was added and the extraction was carried out at 80 to 100 ° C. for 8 hours as an optimal extraction condition. Respectively.
실시예Example 4: 한방소재 첨가 한방소스의 공정별 품질변화 4: Change of quality of herbal sauce added with oriental ingredients
(1) 한방소재적용 한방소스의 개발(1) Development of oriental herb source
실험에 사용된 재료는 닭가슴살(동원), 계피추출물(Bx 14.3), 정향추출물(Bx 16.7), 녹차잎추출물(Bx 23.4), 오미자추출물(Bx 19.4), 상백피추출물(Bx 12.7), 칠리소스(하인즈, USA), 진간장(샘표, Korea), 참기름(사조대림, Korea), 식용유(해표, Korea)를 사용하였다. 1인 분량은 닭가슴살 70 g에 홍피망 16 g, 청피망 16 g, 양파 32 g을 주재료로 하고 칠리소스와 물 15 g을 혼합하여 총 55 g의 소스를 혼합한 것을 대조군으로 하고, 한방소재 첨가량을 달리한 군을 실험군으로 설정하였다.The materials used in the experiment were chicken breast extract, cinnamon extract (Bx 14.3), clove extract (Bx 16.7), green tea leaf extract (Bx 23.4), omija extract (Bx 19.4), bark extract (Bx 12.7) (Heinz, USA), janggangjang (Sampyo, Korea), sesame oil (Sajo borealum, Korea) and cooking oil (Haepyo, Korea) were used. For 1 person, chili sauce and 15 g of water were mixed with 70 g of chicken breast, 16 g of red bell pepper, 16 g of blue pepper and 32 g of onion as main ingredients, and a total of 55 g of the mixture was used as a control. The experimental group was set up with different doses.
닭가슴살에 각각의 소스의 침투를 위해 소스를 혼합한 후 3℃ 냉장고에서 20분간 재워두었다. 그 다음 스팀 컨벡션 오븐(ME 106T, Lainox, Italy)으로 125℃에서 7분간 조리 후 블래스트 칠러(HCM, Lainox, Italy)에서 3℃로 냉각하였다. 냉각 후 1인분(194 g)씩 담아 챔버형 진공포장기(Model T-300,Tower Industry, Korea)로 탈기해 밀봉하였고, 이때 진공포장필름(폴리에틸렌+LLDP+나일론, 200 mm × 300 mm)을 사용하였다. 포장된 한방액상소스는 3℃ 냉장고(TFK279X, GEC, USA)에서 15일 동안 저장하였다. 냉장고의 온도를 지속적으로 모니터링 하였으며 저장된 음식의 품질변화를 측정하기 위하여 각각의 시료를 생산직 후(0일), 3일, 5일, 10일, 15일에 채취하였다. 또한 관능검사를 위해 시료의 재가열 과정이 필요하므로 예비실험을 거쳐 내부온도가 74℃ 이상이 되도록 전자레인지(HS-B422CB, Hauzen, Korea)에서 3분간 가열하였다.The chicken breast was mixed with the sauces for infiltration of the respective sauce, and then allowed to stand in a refrigerator at 3 ° C for 20 minutes. Then, it was cooked in a steam convection oven (ME 106T, Lainox, Italy) at 125 ° C for 7 minutes and then cooled to 3 ° C in a blast chiller (HCM, Lainox, Italy). After cooling, the container was degassed and sealed with a chamber type vacuum packing machine (Model T-300, Tower Industry, Korea) with one serving (194 g), and a vacuum packaging film (polyethylene + LLDP + nylon, 200 mm × 300 mm) . The packaged herbal liquid sauce was stored for 15 days in a 3 ° C refrigerator (TFK 279X, GEC, USA). The temperature of the refrigerator was continuously monitored and each sample was collected at the post-production (0 day), 3 days, 5 days, 10 days, and 15 days in order to measure the quality change of the stored food. Also, since the sample was reheated for sensory evaluation, it was preliminarily tested and heated for 3 minutes in a microwave oven (HS-B422CB, Hauzen, Korea) so that the internal temperature was 74 ° C or higher.
(2) pH와 수분활성도(Aw) 측정(2) Measurement of pH and water activity (Aw)
각 생산단계 및 저장일에 따른 시료의 pH는 시료를 10 g씩 취하여 100 mL의 증류수를 붓고 스토마커 랩 블렌더(LB H3-400G, TMC International, USA)로 균질화한 후 pH 미터(Model 420A, Orion 3 Stars, USA)로 측정하였다. 시료의 Aw 측정은 시료를 각 부위별로 측정하여 스토마커로 균질화한 후 4 g씩 취하여 플라스틱 용기에 담아 Aw-THERM(Model rotronicag, ART, Swiss)로 수분 활성도를 측정하였다.The pH of each sample was 10 g, 100 mL of distilled water was poured into the flask, and homogenized with a Stocker Lab blender (LB H3-400G, TMC International, USA). PH meter (Model 420A, Orion 3 Stars, USA). The Aw of the sample was measured by measuring each sample and homogenizing it with a stomacher. 4 g of each sample was taken in a plastic container and water activity was measured with Aw-THERM (Model rotronicag, ART, Swiss).
한방소재 첨가 액상소스의 배합비 및 생산단계별 Aw와 pH 값은 하기 표 4와 같다. 원재료의 Aw 값은 90.21~96.43, pH 값은 6.07~7.27의 범위를 나타내었다. 혼합단계에서 대조군의 pH 값이 6.37, 실험군 1이 6.29, 실험군 2가 5.94로 p<0.05에서 유의적인 차이를 나타내었고, 수분활성도는 큰 차이를 나타내지 않았다. 한방소재를 첨가하여 소스로 이용한 실험군 1과 2의 pH 값은 혼합단계에서부터 조리 후 냉각단계에 이르기까지 모든 단계에서 대조군에 비해 낮은 값을 나타내었다.The mixing ratio of the liquid source added with the herbal ingredients and the Aw and the pH values according to the production stages are shown in Table 4 below. The Aw value of raw materials ranged from 90.21 to 96.43 and the pH value ranged from 6.07 to 7.27. In the mixing step, the pH value of the control group was 6.37, that of the experimental group 1 was 6.29, and that of the experimental group 2 was 5.94, showing a significant difference at p <0.05, and the water activity was not significantly different. The pH values of the experimental groups 1 and 2, which were added with the herbal ingredients, were lower than those of the control group at all stages from the mixing stage to the post - cooking cooling stage.
생산공정 과정에서의 Aw값은 각 원재료와 소스를 혼합한 단계에서는 다소 높은 수치를 나타내었으나 소스가 침투되고(marinating), 조리와 냉각하는 과정에서 다소 낮아지는 경향을 보였다. 이러한 결과는 조리 시 간장 등의 양념의 삼투현상으로 수분함량의 감소와 조리와 냉각 과정에서 증발된 것으로 생각된다.The Aw value during the production process showed a somewhat higher value at the mixing stage of each raw material and source, but the source tended to be lowered during marinating, cooking and cooling. These results are thought to be due to the osmotic phenomenon of spices such as soy sauce during cooking and to reduce the moisture content and to evaporate during cooking and cooling process.
첨가물
additive
혼합
mix
염지
salt pond
조리
cooking
냉각
Cooling
(3) 미생물 분석(3) Microbial analysis
원재료 및 조리단계별 시료들을 채취하여 생산단계 별 시료의 미생물 분석을 실시하였다. 시료 채취 시 사용되는 도구와 용기 및 실험에 이용된 배지 및 기구는 모두 고압멸균기를 이용하여 무균 처리 후 사용하였다. 시료는 약 100 g씩 멸균백에 채취하여 바로 분석하였다. 각 시료 25 g에 0.85% 생리식염수 225 mL를 붓고 스토마커를 이용하여 균질화시켜 식품공전(KFDA 2010)의 방법에 따라 표준평판 균수를 측정하였다.Samples of raw materials and cooking stages were sampled and microorganisms were analyzed for each stage of production. Tools and containers used for sample collection and media and equipment used in the experiment were all sterilized using a high pressure sterilizer. Samples were taken in a sterile bag of approximately 100 g and immediately analyzed. To 25 g of each sample, 225 mL of 0.85% physiological saline was poured and homogenized using a stomacher, and the number of standard plates was measured according to the method of Food Code (KFDA 2010).
원재료 및 생산단계별 한방소스 닭고기찜의 미생물 분석결과는 아래 표 5와 같다. 원재료인 닭가슴살은 캔의 원재료를 익혀서 생산된 상품으로 본 실험에 이용된 닭가슴살에서는 총균은 검출되지 않았고, 한방추출물에서도 총균은 검출되지 않았다. 생산단계에서는 전반적으로 대조군에 비해 실험군들이 총균수가 다소 감소하는 경향을 나타내었다.Table 5 shows the result of microorganism analysis of steamed chicken meat sauce with raw materials and production stages. The raw chicken breast was produced by cooking the raw material of can, and no bacterium was detected in the chicken breast used in this experiment, and no bacterium was detected in the herbal extract. In the production stage, the total number of bacteria tended to decrease slightly compared to the control group.
첨가물
additive
혼합
mix
염지
salt pond
조리
cooking
냉각
Cooling
실시예Example 5: 저장기간에 따른 품질평가 5: Quality evaluation according to storage period
(1) Aw 및 pH 측정 결과(1) Aw and pH measurement results
저장기간에 따른 닭고기찜의 Aw와 pH 측정 결과는 아래의 표 6과 같다. Aw의 경우 저장 15일까지 94.84~95.24 범위로 대조군과 실험군 1 및 2 모두에서 저장에 따른 유의적 차를 보이지 않았다.The results of Aw and pH measurement of chicken meat steamed according to storage period are shown in Table 6 below. Aw was in the range of 94.84 ~ 95.24 until 15th day of storage, and there was no significant difference in storage between control and experimental groups 1 and 2.
pH 값은 대조군의 경우 0일 6.54에서 저장 15일에는 6.48의 값을 나타내었다. 실험군 1 및 2는 각 저장일 별로 낮아지거나 높아지는 등 불규칙한 경향을 나타내었으나, 대조군과 실험군간의 pH 값은 대체적으로 대조군에 비해 실험군이 낮은 pH를 나타내었다.The pH value of the control group was 6.54 on day 0 and 6.48 on day 15 of storage. The experimental group 1 and 2 showed irregular tendency such as lowering or increasing with each storage day, but the pH value between the control group and the experimental group was generally lower than that of the control group.
(2) 미생물적 품질변화(2) Microbiological quality change
저장기간에 따른 한방소재추출물 한방소스의 대조군과 실험군 모두 저장기간이 증가함에 따라 총균수는 지속적으로 증가하였으며, 대장균군은 저장 15일까지 검출되지 않았다. 대조군은 저장 0일은 2.07에서 지속적으로 증가하여 저장 15일에 2.51의 수치를 나타내었으며, 이는 조리된 식품의 기준인 총균수 5.00, 대장균군수 2.00 이하에 비추어 저장 15일까지 안전한 값을 나타냈다. 또한, 저장기간 동안 대조군에 비해 실험군들이 더 낮은 총균수를 나타냄을 확인할 수 있었다.As the storage period was increased, the total number of bacteria increased continuously in both control and experimental groups. The control group showed a steady increase at 2.07 on the 0th day of storage and a value of 2.51 on the 15th day of storage. The values were stable until the 15th day of storage in the light of the total number of bacteria in the cooked food, 5.00, and E. coli count less than 2.00. In addition, it was confirmed that the experimental group showed lower total number of bacteria than the control group during the storage period.
sample
(3) 항산화성(3) Antioxidant property
식품에 존재하는 불포화지방산은 공기 중에서 쉽게 산화되어 다량의 과산화물을 생성하여 식품의 품질을 저하시킨다. 닭가슴살의 경우 저지방식품이기는 하나 지질 산화에 대한 안정성을 유지하는 것이 제품의 품질을 유지시키는데 매우 중요한 요인이다. 한방소스 첨가 닭고기찜의 항산화성을 알아보기 위해, 저장기간별 산가 및 TBA가를 측정하였다.Unsaturated fatty acids present in foods are easily oxidized in the air to produce large amounts of peroxides, which degrades the quality of the food. In the case of chicken breasts, although it is a low-fat food, maintaining stability against lipid oxidation is a very important factor in maintaining product quality. To investigate the antioxidative properties of steamed chicken meat sauce, the acid value and TBA were measured.
산가는 균질화된 시료 5 g을 정확히 평량하고 에탄올-에테르(1:2) 100 mL를 넣어 10분간 용출시킨 후 페놀프탈레인액을 지시약으로 하여 엷은 홍색이 30초간 지속될 때까지 0.1N 에탄올성 수산화칼륨 용액으로 적정한 다음, 그 소비량을 다음의 식으로 계산하였다.5 g of the homogenized sample was precisely weighed and eluted with 100 mL of ethanol-ether (1: 2) for 10 minutes. The phenolphthalein solution was added to the solution and the solution was diluted with 0.1 N ethanolic potassium hydroxide solution Then, the consumption was calculated by the following equation.
산가 = (5.611×a×f)/sAcid value = (5.611 x a x f) / s
s: 시료 채취량(g)s: Sample weight (g)
a: 0.1N 에탄올성 수산화칼륨 용액의 소비량(mL)a: consumption of 0.1N ethanolic potassium hydroxide solution (mL)
f: 0.1N 에탄올성 수산화칼륨 용액의 역가f: Potency of 0.1N ethanolic potassium hydroxide solution
TBA가는 시료 5 g을 시험관에 취하여 실험진행 과정 중 지방 산화를 중지시키기 위하여 50 g의 BHA(7.2% in ethanol)를 넣은 후 균질기(ULTRA-TURRAX, IKA-WERKE, Germany)로 균질화 시켰다. 균질물 1 mL를 취하여 15 mL 시험관에 넣고 TBA/TCA 용액(20 mM TBA in 15% TCA) 2 mL를 넣은 후 혼합하고 끓는 물에서 15분간 가열하였다. 10분간 냉수에 냉각한 후 다시 혼합하고 원심분리기를 이용하여 3,000 rpm에서 15분간 원심분리하고, 상층액 1 mL를 취하여 UV 분광광도계(GENESYS 10UV, Thermo spectronic, USA)로 532 nm에서 흡광도를 측정한 후 검량선을 이용하여 mg 말론디알데히드(malondialdehyde)의 농도(MDA mg/kg)로 나타내었으며, 모든 실험은 두 번 반복하여 분석하였다.5 g of the TBA thin layer was placed in a test tube and 50 g of BHA (7.2% in ethanol) was added to homogenize with a homogenizer (ULTRA-TURRAX, IKA-WERKE, Germany) Take 1 mL of the homogenate, place in a 15 mL test tube, add 2 mL of TBA / TCA solution (20 mM TBA in 15% TCA), mix and heat in boiling water for 15 minutes. After cooling to cold water for 10 minutes, the mixture was mixed again and centrifuged at 3,000 rpm for 15 minutes using a centrifuge. 1 mL of the supernatant was taken and absorbance was measured at 532 nm using a UV spectrophotometer (GENESYS 10 UV, Thermo spectronic, USA) The concentration of malondialdehyde (MDA mg / kg) was measured using a calibration curve and all experiments were repeated twice.
그 결과, 저장기간이 증가할수록 모든 실험군의 산가(AV)와 TBA가는 증가하였다. 산가의 경우 저장 0일, 대조군은 2.07, 실험군은 2.03~2.09로 한방소재추출물 첨가군에 따른 변화는 나타나지 않았다. 그러나, 저장 15일 차에서는 대조군에 비해 실험군이 낮은 산가를 나타내었다. TBA 값에서도 대체적으로 대조군에 비해 실험군에서 낮은 값을 나타내었다.As a result, the acid value (AV) and TBA of all experimental groups increased with increasing storage period. In the case of acid value, the storage was 0 day, the control group was 2.07, and the experimental group was 2.03 ~ 2.09. However, on the 15th day of storage, the acid value of the experimental group was lower than that of the control group. TBA values were generally lower in the experimental group than in the control group.
(mg KOH/g)AV
(mg KOH / g)
(MDA mg/kg)TBA
(MDA mg / kg)
실시예Example 6: 관능적 품질변화 6: Sensory quality change
한방소재 첨가 한방소스를 이용한 닭고기찜에 대해 관능검사를 실시하였다. 관능검사 요원은 대구한의대학교 한의예과 본초학교실 대학원생과 삼미식품의 연구원 15명으로 소정의 훈련을 거친 후 관능검사를 실시하였다. 관능평가 항목은 냄새(odor), 색깔(color), 맛(taste), 풍미(flavor), 조직감(texture), 전반적 기호도(acceptance)에 대해 평가하도록 구성하였고, 평가 척도는 7점 척도법으로 7점을 가장 좋은 것으로, 4점은 보통, 1점은 가장 나쁜 것으로 평가하도록 하였다.Sensory evaluation of chicken steamed with herbal sauce added. Sensory tests were carried out by a graduate student in the department of herbal medicine, Daegu Haany University and 15 researchers in Sammi Foods. Sensory evaluation items were structured to evaluate odor, color, taste, flavor, texture, and overall acceptance. The evaluation scale consisted of seven points , And 4 were rated as the worst and 1 as the worst.
그 결과, 냄새, 색깔, 맛, 풍미 및 조직감에 대한 기호도에서는 실험군 2가 가장 낮은 점수를 보였고, 냄새, 맛, 풍미 및 조직감에 대한 기호도에서는 실험군 1이 가장 높은 점수를 나타내었다. 전반적인 기호도에서는 대조군 및 실험군 2에 비해 실험군 1이 가장 높은 선호도를 나타내었다.As a result, the lowest scores were obtained in the group 2 for the smell, color, flavor, flavor and texture, while the group 1 for the smell, taste, flavor and texture showed the highest score. In the overall acceptability, the experimental group 1 showed the highest preference compared to the control group and the experimental group 2.
Claims (6)
(b) 정향에 물을 2.7~3.3배량(v/w) 첨가한 후 α-아밀라아제(α-amylase)를 첨가하여 80~100℃에서 6~7시간 동안 추출하여 16.4~17.0 brix의 정향 추출물을 제조하는 단계;
(c) 녹차 잎에 물을 15~19배량(v/w) 첨가한 후 α-아밀라아제(α-amylase)를 첨가하여 80~100℃에서 7~8시간 동안 추출하여 23.0~23.8 brix의 녹차 추출물을 제조하는 단계;
(d) 오미자에 물을 7.5~8.5배량(v/w) 첨가한 후 α-아밀라아제(α-amylase)를 첨가하여 80~100℃에서 5.5~6.5시간 동안 추출하여 19.0~19.8 brix의 오미자 추출물을 제조하는 단계;
(e) 상백피에 물을 10~14배량(v/w) 첨가한 후 α-아밀라아제(α-amylase)를 첨가하여 80~100℃에서 7.5~8.5시간 동안 추출하여 12.4~13.0 brix의 상백피 추출물을 제조하는 단계; 및
(f) 고추 분말 28~36 g, 양파 다이스(dice) 28~36 g, 칠리소스 16~24 g, 간장 8~12 g, 참기름 5~7 g, 양파분말 0.8~1.2 g, 마늘분말 2.5~3.5 g 및 물 12~18 g과 상기 (a)단계의 계피 추출물 0.1~0.3 g, 상기 (b)단계의 정향 추출물 1.4~2.0 g, 상기 (c)단계의 녹차 잎 추출물 0.6~1.0 g, 상기 (d)단계의 오미자 추출물 0.1~0.3 g 및 상기 (e)단계의 상백피 추출물 0.05~0.15 g을 혼합하는 단계를 포함하여 제조하는 것을 특징으로 하는 닭고기찜용 한방소스의 제조방법.(a) Cinnamon extracts of 14 ~ 14.6 brix were prepared by adding water to cinnamon 3.5 ~ 4.5 times (v / w) and then adding α-amylase at 80 ~ 100 ℃ for 6 ~ 8 hours. Producing;
(b) Water was added 2.7 to 3.3 times (v / w) to the clove, and α-amylase was added to the mixture. The extract was then extracted at 80 to 100 ° C. for 6 to 7 hours to obtain a clove extract of 16.4 to 17.0 brix Producing;
(c) 15-19 times (v / w) of water was added to green tea leaves and α-amylase was added to extract the green tea leaves at 80-100 ° C. for 7-8 hours to obtain 23.0 ~ 23.8 brix of green tea extract Lt; / RTI >
(d) 7.5 ~ 8.5 times (v / w) of water was added to Schizandra chinensis, and α-amylase was added to extract the Schizosaccharomyces japonica extract from 19.0 ~ 19.8 brix at 80 ~ 100 ℃ for 5.5 ~ 6.5 hours. Producing;
(e) 10 to 14 times (v / w) water was added to the alfalfa extract and α-amylase was added thereto. The extract was then extracted at 80 to 100 ° C. for 7.5 to 8.5 hours to obtain 12.4 to 13.0 brix Producing; And
(f) 28 to 36 g of red pepper powder, 28 to 36 g of onion dice, 16 to 24 g of chili sauce, 8 to 12 g of soy sauce, 5 to 7 g of sesame oil, 0.8 to 1.2 g of onion powder, (A), 0.1 to 0.3 g of the cinnamon extract of step (b), 1.4 to 2.0 g of the cinnabar extract of step (b), 0.6 to 1.0 g of the green tea leaf extract of step (c) and 0.1 to 0.3 g of the extract of Omiza extract of step (d) and 0.05 to 0.15 g of the extract of the extract of Mulberry bark of step (e).
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