KR101844673B1 - Cosmetic compositions containing niosomes of graviola extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a niozyme stabilized with graviola extract. More specifically, the present invention relates to a cosmetic composition containing a niacin stabilizing graviola extract using sodium palmitoyl sarcosinate, cetyl phosphate and macadamia seed oil, Vesicles, and is excellent in antimicrobial, anti-inflammatory, antioxidant and anti-sebum secretion effects.

Description

[0002] Cosmetic compositions containing niosomes of graviola extract, which stabilize graviola extract,

The present invention relates to a cosmetic composition containing a niozyme stabilized with graviola extract. More specifically, the present invention relates to a cosmetic composition containing a niacin stabilizing graviola extract using sodium palmitoyl sarcosinate, cetyl phosphate and macadamia seed oil, Vesicles and using the same as cosmetics.

Improved living standards due to rapid economic growth have stimulated cosmetics consumption. In recent years, there has been a growing demand for functional cosmetics having specific functions such as moisturizing, wrinkle improvement, and whitening, which are not satisfied with cosmetics used for obtaining simple beauty such as cleanliness and beauty. Accordingly, in the cosmetics industry, efforts are being made to develop formulations capable of promoting transdermal absorption while stabilizing active ingredients with new material development.

Efforts and studies have been actively made to utilize a transdermal delivery system (TDS) in functional cosmetics to stabilize the active substance and to improve transdermal permeation efficiency. TDS technology is a kind of drug delivery system (DDS) technology that is widely used in the pharmaceutical industry. It aims to efficiently absorb and transmit active substances to skin. In the cosmetics industry, efforts are being made to develop innovative cosmetics I am concentrating.

Drug Delivery System (DDS) is a series of techniques that control the delivery and release of drugs to achieve optimal efficacy by using a variety of techniques for substances with pharmacological activity. Vesicles, such as liposomes and Niosomes, have been studied and applied as target-oriented drug delivery systems to enhance bioavailability and achieve long-term therapeutic effects. These liposomes and nioxides are very useful vesicles for the delivery of hydrophilic or hydrophobic drugs and are known to have many advantages such as storage of controlled active ingredients contained in existing formulations, controlled release, and target orientation. Liposomes are produced using phospholipids, which are the main components of biological membranes, and are composed of lipid bilayers similar to skin structures, and thus have excellent permeability to skin and have been widely used in medicines and cosmetics. Liposomes, however, are poorly chemically stable due to the hydrolysis of phospholipids, the major constituent. Therefore, many studies have been made to develop a chemically stable and low-cost active ingredient transporter, and research has been conducted on nioxus, which has similar structure to liposomes but has improved disadvantages.

Niozyme is a lamellar vesicle made by adding cholesterol to a nonionic surfactant. It is similar in structure and use to liposomes and can be used as a carrier of active ingredients. Ionic amphipathic substances can form bilayers in most cases, but they are difficult to apply to the human body due to their charge and toxicity. However, nonionic surfactants are not chargeable and can form bilayers. They are cationic, anionic, Is less toxic than amphoteric surfactants and is suitable as a material for forming nioxos. Cholesterol also plays a role in strengthening the membranes of the endoplasmic reticulum.

Niozyme influences the composition, filling efficiency and release rate of the bilayer depending on the constituents and the manufacturing method. These niobs have many advantages over other endoplasmic reticulum. It is possible to collect both the hydrophilic and hydrophobic substances, and the active ingredient can be stably stored even after the capture. In addition, it is possible to produce nioxides of various sizes depending on the shape of the endoplasmic reticulum, thereby improving the skin permeability.

Korean Patent Laid-Open Publication No. 2016-0044734 entitled " Cosmetic Composition Comprising Twixroutine-Containing Niosomes ", " Polyglyceryl-6 behenate, a nonionic surfactant, and sodium stearoylglutamate, an anionic surfactant, (NSV) is prepared, and a cosmetic composition is prepared which contains tromethyleutine in the niobasic space. Korean Patent Laid-Open Publication No. 2004-0111227 "Vitamin and Amaranth oil-containing niozyme and cosmetic composition containing the same Composition "is to stabilize vitamins and oils unstable in light, heat and air by using phytosterol, cholesterol, ceteth-24, chlores-24, cetyl phosphate, glycerin and ceramide Technology is disclosed.

The present inventors have studied a formulation capable of stabilizing graviola extract having various skin improving activities to maintain its activity for a long time in a formulation and to promote transdermal absorption to increase bioavailability. As a result, it has been confirmed that stability and transdermal absorption rate can be increased when sodium phosphate palmitoyl sarcosinate, cetyl phosphate, and macadamia seed oil are used as nonionic surfactants to complete the present invention.

Therefore, it is an object of the present invention to provide a cosmetic composition containing a niozyme stabilized with graviola extract and having a high dermal absorption rate while maintaining its activity for a long time.

It is another object of the present invention to provide a method for stabilizing graviola extract by preparing a niozyme containing graviola extract.

To achieve the above object, according to the present invention, there is provided a cosmetic composition containing nioxin stabilized with graviola extract in an amount of 0.001 to 20% by weight based on the total weight of the cosmetic composition.

The niose stabilized with the graviola extract may contain 1 to 5% by weight of graviola extract, based on the total weight of the niozyme; 6 to 7% by weight of a mixture of Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate; From 0.65 to 0.8% by weight of sodium palmitoyl sarcosinate; And 8 to 12% by weight of Macadamia ternifolia seed oil.

The niozyme may further contain 5 to 10% by weight of glycerin, 0.01 to 0.05% by weight of disodium EDTA, and the balance water.

The nanosomes stabilized with the graviola extract have a size of 150 to 230 nm.

The cosmetic composition can be used for antibacterial, anti-inflammatory, antioxidant and sebum secretion suppressing.

According to the present invention, there is provided a pharmaceutical composition comprising (A) Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate, Melting 6 to 7% by weight of the mixture at 60 to 80 占 폚; (B) 1 to 5% by weight of graviola extract, 0.65 to 0.8% by weight of sodium palmitoyl sarcosinate, 5 to 10% by weight of glycerin, 0.01 to 0.05% by weight of disodium EDTA and Mixing the remaining amount of water and melting at 60 to 80 캜; (C) 8 to 12% by weight of macadamia ternifolia seed oil at 60 to 80 캜; (D) adding the mixture of step (B) to the mixture of step (A), mixing the mixture at 50 to 60 캜 with adding macadamia seed oil of step (C) And (E) treating the gravurea extract with a high-pressure microemulsifier two to three times in succession at 1000 to 2000 bar.

According to the present invention, when the niozyme is manufactured, the graviola extract is effectively stabilized to maintain its activity for a long time in the formulation, and the bioavailability can be increased by accelerating the percutaneous absorption, and the cosmetic composition containing the same can be used as anti- It can be used effectively as a cosmetic for suppressing secretion.

1 is a graph showing the anti-inflammatory effect of graviola extract.
2 to 5 are graphs showing sebum production inhibitory activity of graviola extract.
6 is a graph showing the stability with time of graviola extract niozyme.

Hereinafter, the present invention will be described more specifically. The present invention is characterized by effectively stabilizing graviola extract as an active ingredient and preparing it as a niozyme to enhance the skin absorption rate and using it as a cosmetic product.

Graviola has been used for chemotherapy, and is known to be effective for skin diseases. It is known that graviola is rich in vitamin C, vitamin B1 and vitamin B2, inhibits the growth of various cancer cells, and has excellent antimicrobial activity to effectively treat bacterial infections.

 In Peru, tea leaves made from graviola are used in the neck, and seeds are used to remove parasites. Peel and root leaves are used as diabetes medications, sedatives, and anticonvulsants. Brazilian Amazon is used to treat liver disease by using leaves, and also uses folk remedies to relieve aches and pains such as neuralgia, rheumatism and arthritis.

According to the present invention, there is provided a cosmetic composition containing 0.001 to 20% by weight of nioxine stabilized with graviola extract, based on the total weight of the cosmetic composition.

First, graviola hydrothermal extracts and ethanol extracts were prepared and their surface components were identified. Various skin improving activities were evaluated using these extracts. As a result, it was confirmed that the 70% ethanol extract showed a higher flavonoid content. The extracts also showed excellent antimicrobial activity, antioxidant activity, antiinflammatory activity, and sebum secretion inhibitory activity.

In order to find a method for effectively stabilizing the graviola extract, stability was confirmed by preparing NLC (Nanostructured Lipid Carriers), Nanoemulsion and Niosome formulations. As a result, it was confirmed to be the most stable in the niozyme formulation.

The niozyme stabilized with the graviola extract of the present invention contains 1 to 5% by weight of graviola extract, based on the total weight of the niozyme; 6 to 7% by weight of a mixture of Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate; From 0.65 to 0.8% by weight of sodium palmitoyl sarcosinate; And 8 to 12% by weight of Macadamia ternifolia seed oil.

At this time, the graviola extract is preferably 70% ethanol extract. The mixture of Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate contains 6 to 7% by weight, based on the total weight of the niozyme , And it was most stable when it contained 6.5% by weight. According to a preferred embodiment of the present invention, 1.5% by weight of Brassica campestris sterols, 3.0% by weight of PEG-5 rapeseed sterol, 1.5% by weight of cholesterol and cetyl phosphate (Cetyl phosphate in an amount of 0.5% by weight, the most stable niozyme was formed.

Sodium palmitoyl sarcosinate, a nonionic surfactant, was stable when it contained 0.65-0.8 wt% based on the total weight of the niozyme, and was most stable when it contained 0.7 wt%.

Also, the Macadamia ternifolia seed oil had a high stability when it contained 8 to 12 wt% based on the total weight of the niozyme, and was most stable when it contained 10 wt%.

The niozyme further contains 5 to 10% by weight, preferably 7.0% by weight of glycerin, 0.01 to 0.05% by weight, preferably 0.01% by weight of disodium EDTA, and the balance water.

According to one embodiment of the present invention, the niozyme stabilizing the gravure extract is prepared as follows.

First, 6 to 7% by weight of (A) a blend of Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate is heated to 60 to 80 ° C . (B) 1 to 5% by weight of prepared graviola extract, 0.65 to 0.8% by weight of sodium palmitoyl sarcosinate, 5 to 10% by weight of glycerin, 0.01 to 0.05% by weight of disodium EDTA, And the remaining amount of water are mixed and dissolved at 60 to 80 ° C. (C) 8 to 12% by weight of macadamia ternifolia seed oil is dissolved at 60 to 80 캜. Next, the mixture of step (B) is added to the mixture of step (A), and the mixture is mixed with macadamia seed oil of step (C) at 50 to 60 ° C. The nanosomes can be prepared by treating with a high-pressure microemulsifier for 2-3 times continuously at 1000-2000 bar to contain graviola extract.

The thus prepared graviola extract is stabilized to have a size of 150-230 nm. Since the nanosize nanosome is manufactured as described above, graviola extract, which is an active substance, can be efficiently delivered to the skin because of high transdermal permeability.

Since the cosmetic composition containing the niacin stabilizes the graviola extract, the graviola extract as an active ingredient can be stably stored for a long period of time in the inside of a niobasal vesicle, and the transdermal permeability is high, It can be effectively used as functional cosmetics for antibacterial, anti-inflammation, antioxidant and sebum secretion suppressing.

The cosmetic composition of the present invention is not particularly limited in its formulation and examples thereof include a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, , A hand cream, an essence, a pack, a soap, a cleansing foam, a cleansing lotion, a cleansing cream, a body lotion, a body cleanser, an emulsion, a press powder, a loose powder or an eye shadow.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to the following examples. However, the following examples are intended to illustrate the contents of the present invention, but the scope of the present invention is not limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

[Example]

Hereinafter, the present invention will be described in more detail with reference to examples and test examples.

Production Example 1: Preparation of graviola hot water extract

After drying, it is pulverized and weighed, so that it is immersed in 50g / 1.25L. Usually, 50g / 1L is extracted. However, in the case of hot water extraction, 1.25L of distilled water is used in consideration of evaporation of distilled water during the heating process. Graviola immersed in distilled water at 50 g / 1.25 L is heated for 90 minutes with a pot. After that, cool the extract and filter it once with a sieve, then filter it with two 25-μm pore size filter paper. Pore size Filter once more with a piece of 5 μm filter paper. The filtrate is concentrated using a rotary evaporator under the conditions of a water bath temperature of 55 ° C and a flask rotation speed of 90 rpm. After fully concentrated samples are recovered, the yield is determined and sealed and refrigerated.

Production Example 2: Preparation of graviola ethanol extract

After drying, it is pulverized and weighed and immersed in 50g / 1L. Thereafter, it is extracted with an overhead stirrer for 3 hours at a room temperature and a rotational speed of 300 rpm. Cool the extract, filter it once with a sieve, and filter it with two 25-μm pore size filter paper. Pore size Filter once more with a piece of 5 μm filter paper. The filtrate is concentrated using a rotary evaporator under the conditions of a water bath temperature of 55 ° C and a flask rotation speed of 90 rpm. After fully concentrated samples are recovered, the yield is determined and sealed and refrigerated.

Test Example 1: Determination of an indicator component of graviola extract

Total phenol content analysis

Gallic acid (97.5%, Sigma) is dissolved in distilled water to prepare a standard solution. To 0.05 ml of 2N Folin-ciocalteu's reagent (Sigma) is added to 0.1 ml of the prepared standard solution, the above-mentioned graviola hot water extract, and 70% ethanol extract. After addition of 0.3 ml of 20% sodium carbonate, the mixture was allowed to stand at room temperature for 15 minutes, adjusted to 1 ml volume with distilled water, and then absorbed at 725 nm by UV / VIS spectrophotometer.

Total flavonoid content analysis

Quercetin is dissolved in ethanol to prepare a standard solution. Take 1.0 ml of the prepared standard and sample in two 15 ml test tubes as a control and test solution. 3.0 ml of ethanol, 0.2 ml of 10% aluminum nitrate solution, 0.2 ml of 1 M potassium acetate solution and 5.6 ml of distilled water are added. Separate standard solutions and samples with distilled water instead of 10% aluminum nitrate solution. After standing at room temperature for 40 minutes, the absorbance of the liquid layer was measured with a UV / VIS spectrophotometer at 415 nm.

The results are shown in Tables 1 and 2, respectively.

sample Absorbance Dilution factor Total phenol concentration Graviola
Hot water extract 0.815 222.222 3.78 Graviola
70% ethanol extract 0.818 240.963 4.12

sample Absorbance Dilution factor Total flavonoid concentration Graviola
Hot water extract 0.011 93.458 0.053 Graviola
70% ethanol extract 0.027 99.010 0.102

From the above results, it was confirmed that the content of phenol and flavonoid was higher in the 70% ethanol extract than in the graviola hot water extract.

Test Example 2: Antimicrobial activity of graviola extract

In order to confirm the antimicrobial activity against E. coli strain, Staphylococcus aureus strain and Staphylococcus epidermis strain known as skin-resident bacteria causing atopy, a 70% ethanol extract of graviola of Preparation Example 2 was treated to produce a clear growth zone, Were measured and compared. The results are shown in Table 3 below.

Strain Concentration
(mg / mL) Clear zone on plate (mm) EE S. aureus 0.25 13 0.5 14 0.75 15 One 16 S. epidermidis 0.25 17 0.5 16 0.75 14 One 18 E. coli 0.25 20 0.5 11 0.75 14 One 19 P. acnes 0.25 18 0.5 18 0.75 18 One 19

Test Example 3: Antioxidant activity of graviola extract

The DPPH method is the most common method for evaluating antioxidant activity and free radical scavenging effect. When it reacts with antioxidant substances such as ascobic acid, aromatic, and amine, which are currently widely used, the absorbance value decreases while returning to a stable form. This DPPH radical scavenging activity is a convenient method for measuring the antioxidant activity of natural materials. DPPH radical scavenging activity was evaluated to evaluate the antioxidative activity of the graviola extract prepared in the above Preparation Example. The results are shown in Tables 4 and 5 below.

Concentration (ppm) Graviola-DW Average % 0 0.608 0.636 0.638 0.627 25 0.617 0.631 0.63 0.626 0.2 50 0.606 0.612 0.612 0.610 2.8 100 0.582 0.577 0.584 0.581 7.4 500 0.311 0.324 0.326 0.320 48.9 1000 0.151 0.151 0.15 0.151 76.0 2500 0.141 0.148 0.144 0.144 77.0 5000 0.169 0.175 0.172 0.172 72.6

Concentration (ppm) Graviola-70% EtOH Average % 0 0.688 0.626 0.627 0.647 25 0.614 0.613 0.602 0.610 5.8 50 0.597 0.598 0.588 0.594 8.1 100 0.571 0.565 0.556 0.564 12.8 500 0.368 0.358 0.352 0.359 44.5 1000 0.238 0.212 0.202 0.217 66.4 2500 0.097 0.092 0.09 0.093 85.6 5000 0.092 0.094 0.096 0.094 85.5

The antioxidant activity of the extracts prepared with 70% ethanol as a solvent was better.

Test Example 4: Inflammation inhibitory activity of graviola extract

Expression and production of cytokines such as PGE2 and TNF-α were investigated. Prostaglandin (PG) is known to be a hormone belonging to unsaturated fatty acids derived from arachidonic acid (AA) produced in the phospholipid present in the cell membrane. Of the PGs generated, PGE2 is deeply involved in allergic inflammation, inhibits tumor cell apoptosis, induces angiogenesis, and contributes to tumorigenesis. To determine whether the production of PGE2, a typical cytokine involved in inflammation induced by allergies, was suppressed by graviola extract, cytokine secretion was measured by ELISA.

TNF-α is a mediator of the inflammatory response and is known to be deeply involved in the early inflammatory response. TNF-α secreted by macrophages and mast cells increases expression in inflammatory lesions as a major mediator of the LPS response. It is cytotoxic to tumor cells. To determine whether the production of TNF-α, a typical cytokine involved in inflammation induced by allergies, was suppressed by graviola extract, cytokine secretion was measured by ELISA. The results are shown in Fig.

PGE2 secretion produced by LPS stimulation was reduced by 14.0% at 50 μg / ml and by 24.0% at 100 μg / ml for the hydrolyzate of graviola. It was confirmed that 32.3% at 50 μg / ml of graviola ethanol extract and 41.9% at 100 μg / ml of graviola ethanol extract.

In addition, it was confirmed that TNF-α secretion produced by LPS stimulation was reduced by graviola extract. It was confirmed that the concentration of graviola hydrothermal extract decreased from 10.6% at 100 μg / ml to 18.8% at 50 μg / ml, 32.5% at 50 μg / ml of ethanol extract of graviola, and 39.1% at 100 ppm.

Test Example 5: Inhibitory activity of sebum production of graviola extract

Identification of effects on growth of sebaceous gland cells

The sebaceous cell line was treated with graviola ethanol extract at various concentrations and MTT assay was performed to confirm the effect on growth. The results are shown in Fig.

Growth inhibition effect was shown at 500 μg / ml of graviola ethanol extract, and growth inhibitory effect was about 15% at the above concentration.

Assessment of inhibition of cell differentiation of sebaceous glands

Lipid droplets were formed by the differentiation of sebaceous cell line. Therefore, the inhibitory effect of lipid droplets on the differentiation inhibition of sebaceous cell line was investigated. The sebaceous cell line was divided into 3 × 10 ⁵ cells / well on a 6-well plate and stabilized for 24 hours. The graviola ethanol extract was treated for 5 days. After incubation, cells are fixed, stained with oil red solution, washed with PBS and dried. After dissolving the staining reagent with isopropanol, 200 μl of the sample was taken in a 96-well plate and absorbance was measured at 520 nm. The results are shown in Fig.

The graviola extract showed the effect of inhibiting the differentiation in a concentration dependent manner. Especially, ethanol extract of graviola showed a significant inhibitory effect at 400 μg / ml concentration.

Assessment of sebum formation inhibition efficacy of sebaceous cells

AdipoRed assay kit was used to investigate the effect of inhibiting the formation of sebaceous glands by inhibiting the formation of triglycerides in sebaceous cells. The sebaceous cell line was divided into 3 × 10 ⁵ cells / well in a 6-well plate and stabilized for 24 hours. The graviola ethanol extract was treated for 5 days. After incubation, the cells were washed with PBS, treated with AdipoRed solution, and incubated for 10 min at room temperature. Absorbance was measured. The results are shown in Fig.

The ethanol extract of graviola showed anti - sebum production inhibitory effect in a concentration - dependent manner. Especially, the inhibitory effect of graviola ethanol extract at 200, 400 μg / ml concentration appears.

Investigation of anti-inflammatory activity of sebaceous cells

After the pretreatment of the graviola ethanol extract with the sebaceous gland cell line, LPS (1 μg / ml) was treated for 24 hours to induce an inflammatory response. Western blot and ELISA were used for expression of each protein. The results are shown in Fig.

The ethanol extract of graviola showed anti - sebum production inhibitory effect in a concentration - dependent manner. Especially, the inhibitory effect of graviola ethanol extract at 200, 400 μg / ml concentration appears. It was confirmed that the ethanol extract of graviola suppressed IL-1β expression in a concentration-dependent manner.

Comparative Example 1: Preparation of NLC Formulation

In order to stabilize the graviola extract prepared in the preparation example, various formulations were prepared. First, Nanostructured Lipid Carriers (NLC) formulations containing graviola extract were prepared.

The A-phase and the B-phase indicated in the prescription of Table 6 are melted at 70 ° C, respectively. Put B phase in A and mix it uniformly at 50 ℃. The NLC formulation was prepared by treating the mixed solution with a high pressure microemulsifier three times in succession at 1000 bar.

Prize Raw material name Graviola
Hot water extract
(weight%) Graviola ethanol extract
(weight%) A Polyglyceryl-3 Methylglucose Distearate 3 3 Hexadecyl hexadecanoate 20 20 Macadamia Ternifolia Seed Oil 5 5 B Glycerin 7.0 7.0 Disodium EDTA 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 0.7 Water to 100 to 100 extract One One C Macadamia Ternifolia Seed Oil 10.0 10.0

However, it was confirmed that layer separation and sediment formation occurred over time and the stability was low.

Comparative Example 2: Preparation of nanoliposome formulation

The A-phase and the B-phase indicated in the prescription of Table 7 are melted at 70 ° C, respectively. Put B phase in A and mix it uniformly at 50 ℃. The mixed solution was treated with a high pressure micro emulsifier three times in succession at 1000 bar to prepare a nanoliposome formulation.

Prize Raw material name Graviola
Hot water extract
(weight%) Graviola
Ethanol extract
(weight%) A Lecithin 5 5 Et-OH 20 20 B Water to 100 to 100 extract One One

However, it was confirmed that layer separation and sediment formation occurred over time and the stability was low.

Example 1: Preparation of a niacin formulation containing graviola extract

Niosome (non-ionic surfactant-based liposome) formulation of graviola extract obtained from hot water and ethanol extraction solvent was prepared.

We tried to prepare the most stable niozyme by varying the components and contents of the niobate formulation. The A phase, B phase and C phase indicated in the prescription of Table 8 below are melted at 70 DEG C, respectively. Add phase B to phase A, mix phase C, and mix uniformly at 50 ° C. The mixed solution is prepared by treating with a high pressure micro emulsifier twice at 1000 bar continuously.

And compared with the stability of the niozyme prepared by varying the content of the A phase.

Prize Raw material name Example 1
(weight%) Comparative Example 3
(weight%) Comparative Example 4
(weight%) A Brassica Campestris Sterols 1.5 1.0 2.5 PEG-5 Rapeseed Sterol 3.0 2.0 3.0 Cholesterol 1.5 1.0 1.5 Cetyl Phosphate 0.5 0.5 0.5 B Glycerin 7.0 7.0 7.0 Disodium EDTA 0.01 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 0.7 0.7 Water to 100 to 100 to 100 Graviola ethanol extract One One One C Macadamia Ternifolia Seed Oil 10.0 10.0 10.0

The content of sterols on A was varied to prepare niozyme. The stability of the formulation was evaluated by measuring the zeta potential. The results are shown in Table 9 below.

division Zeta potential Comparative Example 1
(The sum of the phase A was 5.5% by weight) -14.68 Example 1
(6.5 wt% of the sum of the phase A) -50.48 Comparative Example 2
(The sum of the phase A was 7.5% by weight) -35.52

If the zeta potential is lower than -40 and higher than +40, it can be judged that the niobium is stably distributed. As shown in Table 9, the zeta potential was the most stable in Example 1 in which the sum of the A phase was 6.5% by weight.

EXAMPLE 2, COMPARATIVE EXAMPLES 5-7: Preparation of a niozyme formulation containing graviola extract

The niosomes were prepared in the same manner as in Example 1, and the content of the surfactant Sodium Palmitoyl Sarcosinate used was varied and compared as shown in Table 10 below.

Prize Raw material name Example 1
(weight%) Example 2
(weight%) Comparative Example 5
(weight%) Comparative Example 6
(weight%) Comparative Example 7
(weight%) A Brassica Campestris Sterols 1.5 1.5 1.5 1.5 1.5 PEG-5 Rapeseed Sterol 3.0 3.0 3.0 3.0 3.0 Cholesterol 1.5 1.5 1.5 1.5 1.5 Cetyl Phosphate 0.5 0.5 0.5 0.5 0.5 B Glycerin 7.0 7.0 7.0 7.0 7.0 Disodium EDTA 0.01 0.01 0.01 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 0.8 0.4 0.5 0.6 Water to 100 to 100 to 100 to 100 to 100 Graviola ethanol extract One One One One One C Macadamia Ternifolia Seed Oil 10.0 10.0 10.0 10.0 10.0

The stability of the formulation was evaluated by measuring the zeta potential. The results are shown in Table 11 below.

division Zeta potential Example 1 -55.38 Example 2 -42.26 Comparative Example 5 -25.76 Comparative Example 6 -17.38 Comparative Example 7 -32.24

If the zeta potential is lower than -40 and higher than +40, it can be judged that the niobium is stably distributed. As shown in Table 11, the zeta potential was found to be stable in Examples 1 and 2 containing 0.7% by weight and 0.8% by weight of sodium palmitoyl sarcosinate. In particular, when sodium palmitoyl sarcosinate was 0.7% by weight, Respectively.

Comparative Examples 8 to 10: Preparation of a niozyme formulation containing graviola extract

Nioxomes were prepared in the same manner as described in Example 1, and the kinds of surfactants used were experimented and compared as shown in Table 12 below.

Prize Raw material name Example 1
(weight%) Comparative Example 8
(weight%) Comparative Example 9
(weight%) Comparative Example 10
(weight%) A Brassica Campestris Sterols 1.5 1.5 1.5 1.5 PEG-5 Rapeseed Sterol 3.0 3.0 3.0 3.0 Cholesterol 1.5 1.5 1.5 1.5 Cetyl Phosphate 0.5 0.5 0.5 0.5 B Glycerin 7.0 7.0 7.0 7.0 Disodium EDTA 0.01 0.01 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 - - - Polysorbate 80 - 0.7 - - Sodium Stearoyl Glutamate - - 0.7 - Potassium Cetyl Phosphate - - - 0.7 Water to.100 to.100 to.100 to.100 Graviola ethanol extract One One One One C Macadamia Ternifolia Seed Oil 10.0 10.0 10.0 10.0

The stability of the formulation was evaluated by measuring the zeta potential. The results are shown in Table 13 below.

division Zeta potential Example 1 -45.21 Comparative Example 8 -11.12 Comparative Example 9 -14.68 Comparative Example 10 -23.58

As shown in Table 13, zeta potential was most stable when sodium palmitoyl sarcosinate was used.

Comparative Examples 11 and 12: Preparation of a niozyme formulation containing graviola extract

The same procedure as described in Example 1 was carried out to prepare nioxies, and the contents of the oils used were varied as shown in the following Table 14 and compared.

Prize Raw material name Example 1
(weight%) Comparative Example 11
(weight%) Comparative Example 12
(weight%) A Brassica Campestris Sterols 1.5 1.5 1.5 PEG-5 Rapeseed Sterol 3.0 3.0 3.0 Cholesterol 1.5 1.5 1.5 Cetyl Phosphate 0.5 0.5 0.5 B Glycerin 7.0 7.0 7.0 Disodium EDTA 0.01 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 0.5 0.6 Water to 100 to 100 to 100 Graviola ethanol extract One One One C Macadamia Ternifolia Seed Oil 10.0 5.0 15.0

The stability of the formulation was evaluated by measuring the zeta potential. The results are shown in Table 15 below.

division Zeta potential Example 1 -41.35 Comparative Example 11 -16.39 Comparative Example 12 -33.37

As shown in Table 15, zeta potential was the most stable when 10% by weight of Macadamia ternifolia Seed Oil was contained.

Comparative Examples 13 to 16: Preparation of a niozyme formulation containing graviola extract

The same procedure as described in Example 1 was carried out to prepare nioxides, and the different kinds of oils used were tested and compared as shown in Table 16 below.

Prize Raw material name Example 1
(weight%) Comparative Example 13
(weight%) Comparative Example 14
(weight%) Comparative Example 15
(weight%) Comparative Example 16
(weight%) A Brassica Campestris Sterols 1.5 1.5 1.5 1.5 1.5 PEG-5 Rapeseed Sterol 3.0 3.0 3.0 3.0 3.0 Cholesterol 1.5 1.5 1.5 1.5 1.5 Cetyl Phosphate 0.5 0.5 0.5 0.5 0.5 B Glycerin 7.0 7.0 7.0 7.0 7.0 Disodium EDTA 0.01 0.01 0.01 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 0.5 0.6 0.5 0.6 Water to 100 to 100 to 100 to 100 to 100 Graviola ethanol extract One One One One One C Macadamia Ternifolia Seed Oil 10.0 - - - - Cetyl Ethylhexanoate - 10.0 - - - Caprylic / Capric Triglyceride - - 10.0 - - Demethicone - - - 10.0 - Cyclomethicone - - - - 10.0

The stability of the formulation was evaluated by measuring the zeta potential. The results are shown in Table 17 below.

division Zeta potential Example 1 -51.01 Comparative Example 13 -14.38 Comparative Example 14 -25.37 Comparative Example 15 -35.75 Comparative Example 16 -22.39

As shown in Table 17, zeta potential was most stable when Macadamia ternifolia Seed Oil was used.

Examples 3 and 4: Preparation of a niozyme formulation containing graviola extract

The A phase, B phase and C phase indicated in the prescription of Table 18 below are melted at 70 ° C respectively. Add phase B to phase A, mix phase C, and mix uniformly at 50 ° C. The mixed solution was treated with a high pressure micro emulsifier twice at 1000 bar continuously to prepare a niozyme stabilized graviola extract.

Prize Raw material name Example 3
(weight%) Example 4
(weight%) A Brassica Campestris Sterols 1.5 1.5 PEG-5 Rapeseed Sterol 3.0 3.0 Cholesterol 1.5 1.5 Cetyl Phosphate 0.5 0.5 B Glycerin 7.0 7.0 Disodium EDTA 0.01 0.01 Sodium Palmitoyl Sarcosinate 0.7 0.7 Water to 100 to 100 Graviola hot water extract 4 - Graviola ethanol extract - 4 C Macadamia Ternifolia Seed Oil 10.0 10.0

Zeta potential analysis is an analytical method that can predict the surface potential of particles and stability of dispersion. Generally, it is judged that dispersion is stable when the potential is -40 or less or +40 or more. The zeta potential and the particle size of the graviola extract niozyme prepared in Examples 3 and 4 were measured and the results are shown in Table 19 below.

division Zeta potential Particle size Example 3 -47.89 158.9 nm Example 4 -44.67 167.5 nm

Zeta potential analysis indicated that graviola extract was stable in the niozyme formulation. The graviola extract nanosomes of Examples 3 and 4 were confirmed to have a nanoparticle size of 158.9 nm and 167.5 nm. Therefore, graviola extract nanosome formulation is easy to percutaneous absorption.

The pH change was measured for 4 weeks to confirm the stability of the formulation, and the results are shown in FIG. As a result of pH measurement for 4 weeks, no significant difference was found, indicating that the stability over time was excellent.

Formulation Example 1: Preparation of Toner Containing Nioose Stabilized with Graviola Extract

A toner containing a nioose stabilized graviola extract was prepared by a conventional method according to the composition shown in Table 20 below.

Prize Raw material name Content (% by weight) A Water to 100 1,3-Butylene glycol 2.0 Chelating agent 0.05 B Ethyl alcohol 15 Graviola extract
(Example 3) 10 C Ethyl alcohol 5 D Additive One E Fragrance 0.1

Formulation Example 2: Preparation of Lotion Containing Nioose Stabilized with Graviola Extract

A lotion containing a nioose stabilized with graviola extract was prepared in a conventional manner according to the composition shown in Table 21 below.

Prize Raw material name Content (% by weight) A Water to 100 1,3-Butylene glycol 2.0 Chelating agent 0.05 B Emulsifier 3.2 Viscosity agent 0.13 Oil (Plant) 8.0 C Graviola extract
(Example 3) 10 Fragrance 0.2 D Ethyl alcohol 10

Claims (5)

A cosmetic composition comprising a nioxin stabilized with graviola extract in an amount of 0.001 to 20% by weight based on the total weight of the cosmetic composition,
The niose stabilized with the graviola extract may contain 1 to 5% by weight of graviola extract, based on the total weight of the niozyme; 6 to 7% by weight of a mixture of Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate; From 0.65 to 0.8% by weight of sodium palmitoyl sarcosinate; And 8 to 12% by weight of Macadamia ternifolia seed oil. delete The cosmetic composition according to claim 1, wherein the graviola extract is stabilized to have a size of 150 to 230 nm. The cosmetic composition according to claim 1, wherein the cosmetic composition is for antibacterial, anti-inflammatory, anti-oxidative, and sebum secretion-suppressing. (A) 6-7% by weight of a blend of Brassica campestris sterols, PEG-5 rapeseed sterol, cholesterol and cetyl phosphate at 60-80 ° C Melting step;
(B) 1 to 5% by weight of graviola extract, 0.65 to 0.8% by weight of sodium palmitoyl sarcosinate, 5 to 10% by weight of glycerin, 0.01 to 0.05% by weight of disodium EDTA and Mixing the remaining amount of water and melting at 60 to 80 캜;
(C) 8 to 12% by weight of macadamia ternifolia seed oil at 60 to 80 캜;
(D) adding the mixture of step (B) to the mixture of step (A), mixing the mixture at 50 to 60 캜 with adding macadamia seed oil of step (C) And
(E) treating the gravurea extract with a high-pressure microemulsifier at a rate of 2 to 3 consecutive times at 1000 to 2000 bar.

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