KR101842577B1 - Method preparing the Herbal Medicine Mixture through Cryogenic Micro Grinding Technology - Google Patents

Abstract

The present invention relates to a method for producing a medicinal herb mixture ensuring enhanced extractable content of insoluble components by pulverizing herb medicinal ingredients into microparticles using a cryogenic micro grinding technology (CMGT). According to the present invention, by using the CMGT, four types of herb medicinal ingredients included in a Samultang (a traditional medicine for premenstrual and menopausal syndrome in women) can be obtained, which are: Rehmanniae Radix Preparata powder with particle size of 5-10 μm; Paeonia japonica (Makino) Miyabe & Takeda powder with particle size of 4-8 μm; cnidium powder with particle size of 3-7 μm; and dong quai powder with particle size of 3-7 μm. The obtained herb medicinal herb mixture has high extractable content of insoluble and lipophilic components of Rehmanniae Radix Preparata, Paeonia japonica (Makino) Miyabe & Takeda, cnidium, and dong quai, thereby maximizing effectiveness of herb medicinal ingredients.

Description

TECHNICAL FIELD The present invention relates to a method for preparing a mixture of herbal medicines using ultrafine pulverization,

The present invention relates to a method for preparing a mixture of herbal medicines by pulverizing herb medicines with microparticles using a cryogenic microgrinding technology (CMGT), thereby increasing the extraction amount of insoluble components.

In line with today's well-being culture, there is an active movement to promote health by taking a bath made of a mixture of several herbal medicines.

For example, 'Sam-tang', which has been recently published, is a mixture of four kinds of medicinal ingredients, namely, Angelica japonica, Sukjwanghwang, Tanggung, and Baekjaek. It is a blood-forming agent that produces blood and improves blood flow. It helps to remove the eosinophilia in the uterus after delivery and helps the uterus of the growing female.

However, there is a problem that it is not easy to take the hot water, which is prepared by mixing a plurality of herbal medicines such as sametang, because it is poor in convenience and convenience in daily life.

On the other hand, most of the physiologically active ingredients of medicinal herbs are mostly lipophilic substances. The herbal medicinal herb liquid obtained by the conventional hot water extraction method only extracts the water-soluble substances contained in the medicinal herbs. Insoluble and lipophilic substances are not dissolved properly in the hot water extraction, There is a problem.

Accordingly, the present inventor intends to solve such a problem by using a cryogenic ultra-fine grinding technique which has been patented as a registered patent No. 10-1592432.

Here, the cryogenic ultra-fine grinding technique, which has been patented in Patent No. 10-1592432, is a method in which vegetable or fruit is frozen and pulverized at a temperature of -70 to -150 캜 to a size of 15 to 40 탆, liquid nitrogen at -190 to -200 캜 The present inventors have succeeded in extracting the main ingredients of herb medicine by using this technique to maximize the effect of herb medicine and to make it easier to ingest the herb medicine than the conventional herb medicine. To make an ultrafine powdered herbal medicine mixture.

An object of the present invention is to provide a method for preparing a mixture of herbal medicines by pulverizing herb medicines with microgranules using a cryogenic micro grinding technology (CMGT) to increase the extractable content of insoluble components.

In order to attain the above object, the present invention provides a method for preparing a safflower-like ultrafine powder by freezing grinding safflower at a temperature of -130 ° C. to -160 ° C. to an average particle size of 5 to 10 μm; Freezing powder at a temperature of -95 ° C to -120 ° C to an average particle size of 4 to 8 μm to obtain ultrafine powdered whey powder; Freezing and crushing the mantle to a size of 3-7 占 퐉 at an average particle size of -90 占 폚 to -100 占 폚 to obtain an ultramarine ultrafine powder; And Angelica keiskei koidz. At an average particle size of from 3 to 7 mu m at a temperature of from -90 DEG C to -100 DEG C to obtain an Angelica gigas superfine powder, ≪ / RTI >

In this case, the method for preparing the ultrafine powder mixture of Sulfur Sulfur, Veterinary Sulfate, Astragalus membranaceus and Angelica gigas can be interpreted as a method for preparing the medicinal herb mixture comprising the steps of obtaining each of Sulfur, Sulfur, Of course.

The ultrafine powder according to the present invention, i.e., the herbal medicine mixture, may be characterized in that the fat-soluble component of Sulfur Sulfur, Baekjanggi, Tanggu, or Angelica gigas is increased, and the fat-soluble component is selected from the group consisting of Demethylsuberosine, And may be any one selected from the group consisting of Z-Ligustilide, Decursin and Decursinol angelate.

Meanwhile, the present invention provides a pharmaceutical composition for preventing or treating inflammatory diseases, a pharmaceutical composition for improving / treating inflammation diseases, and a health functional food comprising the mixture of ultrafine powder obtained by the above method, i.e., a mixture of herbal medicines.

In the present invention, the pharmaceutical composition may be administered orally, subcutaneously, intravenously, intramuscularly, intranasally or intraperitoneally and may be in the form of tablets, capsules, granules, pills, syrups, solutions, emulsions, suspensions, . ≪ / RTI > The health functional food may be formulated into one selected from the group consisting of tablets, pills, powders, granules, powders, capsules and liquid formulations, further comprising at least one of carriers, diluents, excipients and additives.

According to the present invention, four medicinal herbs contained in a sweet pot are prepared by cryogenic superfine pulverization. The powder is classified into 5 ~ 10 ㎛ seed powder, 4 ~ 8 ㎛ seed powder, 3 ~ 7 ㎛ seed powder, And the resulting herbal medicine mixture has a high extractable content of insoluble and fat-soluble components of Sulfur Sulfur, Baekjanggi, Gyungguk, and Angelica giganta, so that the effect of the medicinal ingredient can be maximized.

1 shows a process for preparing a mixture of herbal medicines using a cryogenic ultra-fine finely divided powder, more specifically, a fine powder of safflower.
FIG. 2 shows the results of HPLC analysis of Sam's hot water extract through hot water extraction.
FIG. 3 shows the results of HPLC analysis of the fine powder of Satsumang using the ultra-low temperature micro-finest method.

Although the scope of the present invention is limited to the preparation of a mixture of medicinal herbs using cryogenic superfine pulverization, for the sake of more detailed explanation, a kind of medicinal herb which is one kind of medicinal herb is exemplified.

Generally, Sam-tang contains four medicinal ingredients: Angelica japonica, Silla japonica, Silla japonica, and Veterinary Medicine. It is a blood-forming agent that produces blood and improves blood flow. It eliminates menstrual irregularities and chills in the lower abdomen. And it helps women who are growing up to help their uterus grow well.

Here, Angelicae Gigantis Radix is Angelica sinensis D., a perennial plant of Umbelliferae, and Angelicae gigas and Ligusticum acutilobum are medicines in Korea. The ingredients are mainly fatty substances such as Bergaptene, butylidenephthalide, and fatty substances and vitamins, and have analgesic, drainage, hemostatic, and tonic effects. Therefore, It is used for women's diseases.

Also, Cnidii Rhizoma is also known as incense, horseshoe, horseshoe, and alum (Umbelliferae), a dried herbaceous plant of Lingustieum chuangxiong (Cnidium officinale), which is a representative medicinal product for circulating blood and vegetables. It contains refined oil, cnidriide, cedaroid acid, amino acid, alkaloid and ferulic acid. It improves symptom such as menstrual pain, menstrual cramps, headache, abdominal pain, dizziness, menstrual irregularity, It is also used for bruises due to thalidomide. Pharmacological tests revealed sedation, blood pressure lowering, antibacterial and uterine contraction.

In addition, Rehmanninae Rhizoma Preparat is the root of Rehmannia glutinosa (Gaertner Libosch.) Or R. glutinosa Libosch. Var. Hueichingensis (Chao et Schih) Hsia. It is dry with steamed stem. It has a bitter taste and a warm nature while it has a flavor. It has a characteristic of bloating and replenishing the base (substance that is the basis for life and activity) It has the effect of treating the symptoms and darkening the head.

On the other hand, Paeoniae Radixa is the root of Paeonia japonica belonging to the peonies and Paeoniaceae and is used as a convergence, relief, shaking and pain medicine in one room. The major components of the veterinary medicine are paeoniflorin, oxypaeoniflorin, benzoylpaeoniflorin, albiflorin, paeoneol, and the like. Anti-inflammatory, anti-stress, and hepatotoxic effects were studied in the peptides, and anti-cancer and immune-promoting effects of the fractions were reported.

The cryogenic micro grinding technology used in the present invention for producing such a sweet pot is to freeze the sample to a cryogenic temperature of -190 to -200 ° C and then crush the sample into fine particles that are finer than flour, The nutrition, flavor and aroma of the water are utilized, and the water is well dispersed in the water as a micro-tenant so that the drinking feeling is good.

According to the results of the HPLC analysis of the ultrafine powder of Samtung cryogenic powder according to the present invention, it was confirmed that Demethylsuberosine of Danggui, Z-Ligustilide of Danggu, Dercusin decursin, Lipid soluble components such as decursinol angelate were extracted.

Here, Decker Shin is a natural component of pyranocoumarin, which was first isolated from the ether extract of Angelica decursiva Fr. et Sav. In Japan. It has a molecular weight of 328.35 and a molecular formula of C ₁₉ H ₂₀ O ₅ .

In addition, decascinol angelate is a structural isomer having the same molecular weight and the same chemical formula as decacin, Z-ligustilide has a molecular weight of 190.24 and a molecular formula of C ₁₂ H ₁₄ O ₂ .

[Compound detected in ultrafine powder at a cryogenic temperature of Samarium]

Meanwhile, the health functional food according to the present invention may be formulated into one selected from the group consisting of tablet, pill, powder, granule, powder, capsule and liquid formulation further comprising at least one of carrier, diluent, excipient and additives .

Examples of foods to which the extract of the present invention can be added include various foods, powders, granules, tablets, capsules, syrups, drinks, gums, tea, vitamin complexes, and health functional foods. Examples of the additive that can be further included in the present invention include natural carbohydrates, flavors, nutrients, vitamins, minerals (electrolytes), flavors (synthetic flavors, natural flavors and the like), colorants, fillers (cheese, At least one component selected from the group consisting of fumaric acid and its salt, alginic acid and its salts, organic acid, protective colloid thickener, pH adjusting agent, stabilizer, preservative, antioxidant, glycerin, alcohol, .

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tautatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharine, aspartame, etc.) can be advantageously used as the flavor.

In addition to the above, the composition according to the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies (cheese, chocolate etc.), pectic acid and its salts, A salt thereof, an organic acid, a protective colloid thickener, a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like.

In addition, the composition according to the present invention may contain flesh for the production of natural fruit juices and vegetable drinks. These components may be used independently or in combination.

Specific examples of the carrier, excipient, diluent and additives include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, erythritol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium phosphate, calcium Silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, polyvinylpyrrolidone, methylcellulose, water, sugar syrup, methylcellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate And a mineral oil are preferably used.

When the health functional food according to the present invention is formulated, it is prepared using diluents or excipients such as fillers, extenders, binders, humectants, disintegrants, surfactants and the like which are usually used. The content of the extract according to the present invention as an active ingredient in the above-described formulation can be appropriately adjusted depending on the mode and purpose of use, the condition of the patient, the type of symptoms and the severity, and is 0.001 to 99.9% by weight, But it is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to the following examples. The following examples also relate to Sam-tang, which is a kind of mixture of herbal medicines, as described above. It will be apparent to those skilled in the art that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention.

Example  One

Manufacture of ultrafine powder at cryogenic temperature

60 g of Suksori was pulverized at an average particle size of 5 to 10 탆 at a temperature of -130 캜 to -160 캜 and 60 g of the pesticide was pulverized at an average particle size of 4 to 8 탆 at a temperature of -95 캜 to -120 캜, At an average particle size of 3 to 7 mu m at 90 DEG C to -100 DEG C, and 60 g of Angelica keiskei koidz. Was crushed to a size of 3 to 7 mu m in average particle size at -90 DEG C to -100 DEG C.

Example  2

Comparison of components of celestial ultrafine powder and Sam-tan bath

The Sangmangtang water solution was prepared by putting 60 g of each of Sukjunghwang, Baekjaejeon, Kyeongguk, and Angelicae into a nonwoven fabric, adding 1.98 L of purified water after putting the nonwoven fabric into a stool, and heating at 100 ° C for 2 hours and 30 minutes. HPLC analysis was performed for the comparison of the composition of the prepared Sangam hot water solution and the mixture of the silicate cryogenic ultrafine powder prepared in Example 1 (Instrument: Agilent HPLC, Column: Phemnomenex Luna 5u C18 (2) 100A 150 x 4.60 mm 5micron , Intection volume: 10 μL, Flow rate: 1.0 mL / min, Detection UV: 230 nm, Mobile phase: Water-MeOH Gradient elution). The sample of Sangmangtang was analyzed by syringe filter and analyzed. Sodium cryo-ultrafine powder samples were analyzed by adding 10mL of extraction solvent to 1.21g of sample and filtering with syringe filter. Distilled water, 50% methanol and 100% methanol were used as extraction solvents. (Methanol is a widely used solvent for extraction of herbal ingredients).

5-HMF, Paeoniflorin, and Nodakenin were detected in the Sangmang-tang water bath. In the case of extracting ultrafine powder samples of Sam-tang cryogenic temperature, . When methanol was added to the sample at the cryogenic temperature ultrafine powder, the content of demethylsuberosine, Z-Ligustilide, decursin and decursin, (Decursinol angelate) compound was detected. The pak area values of the detected compounds are shown in Table 1 below.

5-HMF Paeonia florin Noda cannine Dimethylsuberosine Ligustylide Decker Shin
And dexacinol angelate Sankamang 257.6 702.4 227.5 N.D. N.D. 41.3 Samantang
Cryogenic ultra fine
powder Water extraction 134.3 911.5 114.3 N.D. 21.9 72.9 50% methanol extract 128.2 958.2 124.2 71.2 326.5 4403.8 100% methanol extract 118.4 532.5 125.7 226.0 376.4 5621.2

Claims (12)

(a) freezing grind slurry at a temperature of -130 캜 to -160 캜 at an average particle size of 5 to 10 탆 to obtain ultramarine ultrafine powder;
(b) cryoprotecting the vinegar at a temperature of -95 ° C to -120 ° C to an average particle size of 4 to 8 μm to obtain ultrafine powdery fine powder;
(c) cryogenically crushing the crown to a size of from 3 to 7 mu m at a temperature of from -90 DEG C to -100 DEG C to obtain a celestial ultrafine powder;
(d) freezing and crushing the Angelica gigas at an average particle size of 3 to 7 占 퐉 at a temperature of -90 占 폚 to -100 占 폚 to obtain Angelica giga fin powder, wherein the ultrafine powder of the herbal medicine is extracted Wherein the content of the fat-soluble ingredient is increased.
delete delete delete delete The method according to claim 1,
The fat-
Characterized in that it is any one selected from the group consisting of demethylsuberosine, Z-Ligustilide, Decursin and Decursinol angelate.
A method for preparing a mixture of herbal medicines. delete delete delete delete delete delete

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