KR101077523B1 - A composition comprising the extract of crude drug selected from Eriobotryae Folium and Dendropanax morbiferafor treating and preventing neuro-degenerative disease - Google Patents

Abstract

The present invention relates to a composition having a protective effect on brain neurons and brain function, and more particularly, to a pharmaceutical composition for the treatment and prevention of degenerative brain diseases, containing as an active ingredient one or more herbal extracts selected from the group consisting of yellow chile and non-leaflets. It is about.

Hwangchil Tree, Non-leaflets, Brain Cell Protection, Degenerative Brain Disease

Description

A composition comprising the extract of crude drug selected from Eriobotryae Folium and Dendropanax morbifera for treating and preventing neuro-degenerative disease }

The composition containing the Hwangchil-tree and / or non-leaflet extract mixture of the present invention as an active ingredient exhibits radical scavenging ability, protective effect on brain cells, and cognitive improvement, and thus may be useful for the treatment and prevention of degenerative brain diseases.

[Reference 1] Jeong GS et al, Planta Medica, 74, pp. 1368-1373, 2008

Jin DQ et al, Biochemical and Biophysical Research Communications, 331, pp. 1264-1269, 2005

[3] Lim CS et al, Biological and Pharmaceutical Bulletin, 29, pp. 1212-1216, 2006

Nishioka et al., Biological and Pharmaceutical Bulletin, 25: 1053-1057 (2002)

Taniguchi et al., Phytochemistry, 59: 315-323 (2002)

Shimizu et al., Chemical and Pharmaceutical Bullentin,

     34: 2614-2617 (1986)

De Tommaasi et al., Planta Medica, 57: 414-416 (1991)

8, Jung et al., Archives of Pharmacal Reasearch, 22: 213-218.

     (1999)

Taniguchi et al., Phytochemistry, 59: 315-323 (2002)

10 Blios, M.S., Nature, 395 (26): 11991200 (1958)

[11] Yun-Lian Lina, et al., J. Ethnopharmacology, 122; 417-425 (2009)

Rong Ma, et al., Neuropharmacology, 56: 10271034 (2009)

The present invention relates to a composition having brain cell protection and cognitive function improving activity, and more particularly to a composition for improving brain cell protection and cognitive function comprising one or more herbal extracts selected from the group consisting of hwangchil and non-leaflets. .

Since dementia is a degenerative disease that deteriorates human nature and is a long-term degenerative disease, passive methods of accommodating patients cannot bear the socioeconomic burden. Therefore, active attempts should be made to develop preventive and causative therapies. However, to date, no therapeutic agents have been developed to treat the underlying cause of Alzheimer's disease.Available as general therapeutic agents are Aricept, Pfizer's Aricept, Novartis's Exelon, and Janssen. And Lundbeck's Ebixa (Memantine), a mechanism of antagonists of Reminyl and the recently approved NMDA receptor by the US FDA. However, acetylcholine esterase inhibitors only improve the cognitive ability that has been impaired and do not cure the underlying cause of Alzheimer's disease. In addition, only a few patients have a temporary symptom relief effect, the drug does not last long, so it is difficult to be a fundamental treatment. In addition, the nature of the disease requires long-term administration, the drug is also accompanied by various side effects, including liver toxicity, vomiting, loss of appetite has also been revealed as a problem. Therefore, the development of a therapeutic agent that can prevent the progress of the disease is an urgent task. Many multinational pharmaceutical companies are investing heavily in research and development in this area, especially beta or gamma secretase inhibitors, which reduce the production of beta amyloid, which consists of about 40 amino acids that are believed to be the primary cause of Alzheimer's disease. Development is the dominant. In Korea, basic research on Alzheimer's disease has been conducted to some extent, but there is almost no case in developing dementia treatment itself.

Oxidative stress is known to be caused by imbalance between the oxidative and antioxidant systems in the body due to the generation of free radicals (ROS) in the body or the deterioration of the antioxidant system (Jeong GS et al, Planta Medica, 74, pp. .1368-1373, 2008). As the standard of living improves and the life span of humans increases, interest in aging and various diseases related to aging is increasing, and oxidative stress has been found to be an important factor in degenerative brain diseases of the central nervous system such as Alzheimer's syndrome, Parkinson's syndrome, and Huntington's syndrome. (Jin DQ et al, Biochemical and Biophysical Research Communications, 331: 1264-1269, 2005; Lim CS et al, Biological and Pharmaceutical Bulletin, 29: 1212-1216, 2006).

Hwangchil- tree ( Dendropanax morbifera Lev.) Is a plant belonging to the family arboraceae , and it is an evergreen broad-leaved arboreous tree that grows only in southern coastal areas such as Haenam, Wando, and Jeju Island in Korea. Hwangchil (黃 漆) is called. It has been reported that Hilchi chinensis is effective in removing the heat, and treating the skin and burns. In recent years, it has been reported that the leaves of Hwang Chilchi are effective in anticancer activity and also have antioxidant and nerve stabilizing effects. It is known that Hwangchil-tree extract helps skin whitening by inactivating tyrosinase, which produces melanin pigment in the skin. Hwangchil-tree consists of 66.7% of non-volatile components, 10.8% of aromatic components, 8.1% of moisture, and 14.4% of solids. Especially, the aromatic components are mainly β-cubebene, γ-selinene, and δ-cardi. It is reported to consist of cadiene.

Loquat leaf ( Eriobotryae Folium ) is the leaf part of loquat ( Eriobotrya japonica ), an evergreen tree belonging to the Rosaceae family. In oriental medicine, fruits, roots, stem bark and leaves are used for medicinal purposes. Non-leaflets are known to be effective in clearing the lungs, harmonizing the stomach, strengthening the group, and cutting down the walls. In addition, pharmacological activities such as inflammation-related skin diseases, cough, sputum, hypoglycemia, liver function improvement, and anti-inflammatory activity have been reported (Nishioka et al., Biological and Pharmaceutical Bulletin, 25: 1053-1057 (2002)). The major components of non-leaflets are high in triterpenoid compounds, including ursolic acid, oleanolic acid, maslininic acid, and tometic acid (Taniguchi et al., Phytochemistry, 59: 315-323 (2002)). It has been reported to contain polyphenols such as sesqueterpene glycosides and flavonoid glycosides (Shimizu et al., Chem. Pharm. Bull., 34: 2614-2617 (1986)); De Tommaasi et al., Planta Medica, 57: 414-416 (1991); Jung et al., Archives of Pharmacal Reasearch, 22: 213-218 (1999); Taniguchi et al., Phytochemistry, 59: 315-323 (2002)).

Accordingly, the present inventors have an excellent antioxidative effect on the oxidative stress caused by amyloid beta, which is one of the causes of degenerative brain disease, and the effect of protecting the brain cells and improving brain function. The present invention was completed by confirming that it is useful for the treatment and prevention of brain diseases.

In order to solve the above object, the present invention provides a pharmaceutical composition for the treatment and prevention of degenerative brain disease, containing as an active ingredient one or more herbal extracts selected from the group consisting of yellow lacquer and non-leaflets.

In another aspect, the present invention provides a health functional food for the prevention and improvement of degenerative brain disease, containing as an active ingredient one or more herbal extracts selected from the group consisting of Hwangchil wood and non-leaflets.

When combining the hwangchil wood and non-leafed leaves with each other, the mixed dry weight ratio of the hwangchil-tree and non-leafed leaves is 1 to 10: 1 (w / w), preferably, 1 to 5: 1 (w / w), more preferably 1 It is characterized by mixing at a mixing ratio of ˜2: 1 (w / w).

       The extract may be a lower alcohol such as water, methanol, ethanol, butanol, or a mixed solvent thereof, preferably, water or a mixed solvent of 1-100% water and ethanol, more preferably, about 1-70% water and ethanol Extracts soluble in the solvent.

       The degenerative brain disease is characterized by including stroke, stroke, Alzheimer's disease, Alzheimer's disease, Parkinson's disease, or Huntington's disease, specifically, Alzheimer's disease or Parkinson's disease.

       Hereinafter, the method of obtaining the extract of the present invention will be described in more detail.

After washing and drying Hwangchil wood and non-leafed leaves, preferably domestic, more preferably southern cultivars including Jangheung, Jeonnam, about 1 to 30 times the weight of the sample of Hwangchil wood and non-leafed, preferably about 5 to 15 times (w / v) volume of water, C ₁ to C ₄ lower alcohols or mixed solvents thereof, preferably water or ethanol, more preferably water or 5 to 80% ethanol For example, conventional extraction methods such as heat extraction, ultrasonic extraction, and reflux extraction, for about 1 to 6 hours, preferably 3 to 5 hours, at a reaction temperature of about 70 to 120 ° C, preferably 80 to 110 ° C, are preferred. Preferably, a second step of extracting 1 to 10 times, preferably 2 to 7 times by heat extraction; A third step of filtering and extracting the extract obtained in the above step under reduced pressure; The concentrated extract is lyophilized at about −50 ° C. to −30 ° C., preferably at −45 ° C. to −25 ° C. for about 24 to 72 hours, preferably 40 to 55 hours. Hwangchil-tree and non-leaflet extract of the present invention can be obtained through the step comprising a, respectively, if necessary, the mixed dry weight ratio of Hwangchil-tree and non-leaflet is 1 to 10: 1 (w / w), preferably, 1 to 5: The extracts of the present invention can be obtained by mixing at a mixing ratio of 1 (w / w), more preferably 1 to 2: 1 (w / w).

In another aspect, the present invention provides a pharmaceutical composition and health functional food for the treatment and prevention of degenerative brain disease, containing as an active ingredient at least one herbal extract selected from the group consisting of Hwangchil wood and non-leaflets prepared by the production method and the manufacturing method. do.

One or more herbal extracts selected from the group consisting of Hwangchil-tree and non-leafed leaves are useful for the treatment and prevention of degenerative brain diseases by exhibiting excellent protective effect, antioxidant protection effect and brain function improvement from beta amyloid-induced brain cell toxicity. Do.

The composition of the present invention comprises 0.1 to 50% by weight of the herbal extract based on the total weight of the composition.

      However, the composition is not limited thereto, and may vary depending on the condition of the patient, the type of disease, and the progress of the disease.

The composition comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Compositions comprising extracts according to the invention are formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories and sterile injectable solutions, respectively, according to conventional methods. The carriers, excipients and diluents that may be incorporated therein include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium Silicates, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and such solid preparations may contain at least one excipient such as starch, calcium carbonate, sucrose, or the like. ) Or lactose, gelatin and the like are mixed. In addition to simple excipients, lubricants such as magnesium styrate talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

     Preferred dosages of the extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the extract is preferably administered at 0.01 mg / kg to 10 g / kg, preferably 1 mg / kg to 1 g / kg per day. The administration may be carried out once a day or divided into several doses. Therefore, the above dosage does not limit the scope of the present invention in any aspect.

      The composition of the present invention may be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration can be expected, for example by oral and rectal or intravenous methods.

In another aspect, the present invention provides a health functional food for the prevention and improvement of degenerative brain disease, containing as an active ingredient one or more herbal extracts selected from the group consisting of Hwangchil wood and non-leaflets.

Health functional foods containing the extract of the present invention can be used in a variety of drugs, foods and drinks for the prevention and improvement of degenerative brain diseases. Examples of the foods to which the extract of the present invention can be added include various foods, beverages, gums, tea, vitamin complexes, health supplements and the like, and they can be used as powders, granules, tablets, capsules or beverages have.

Accordingly, the present invention provides a food and food additive containing at least one herbal extract selected from the group consisting of the yellow lacquer tree and non-leaflets having an effect of preventing and improving degenerative brain disease.

Extract of the present invention may be added to food or beverages for the purpose of preventing and improving degenerative brain diseases. At this time, the amount of the extract in the food or beverage is generally added to the health food composition of the present invention to 0.01 to 15% by weight of the total food weight, the health beverage composition is 0.02 to 10 g based on 100 ml, preferably Can be added in a ratio of 0.3 to 1 g.

      The health beverage composition of the present invention contains a mixture of the extract as an essential ingredient in the indicated ratio, and there is no particular limitation on the liquid component, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. have. Examples of the above-mentioned natural carbohydrates include monosaccharides such as disaccharides such as glucose and fructose such as maltose, sucrose and the like and polysaccharides such as dextrin, cyclodextrin and the like Sugar, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

As described above, the composition containing one or more herbal extracts selected from the group consisting of hwangchil wood and non-leaflets of the present invention as an active ingredient exhibits excellent protective effect, antioxidant protection effect and brain function improvement effect from beta amyloid-induced brain cell toxicity As a result, it may be useful for the treatment and prevention of degenerative brain diseases.

Hereinafter, the present invention will be described in detail by the following Examples and Experimental Examples. However, this is only to illustrate the present invention, the content of the present invention is not limited thereto.

Example 1 Preparation of Hydrothermal Extracts of Hwangchil Tree and Non-leafed Leaves

After drying the dried Hwangchil trees and non-leaved leaves (produced in Jangheung, Jeonnam), 36 L of Hwangchil wood samples were added to 36 L of purified water, and extracted by heating at 100 ° C for 4 hours, and the filtrate was concentrated under reduced pressure. Freeze-dried at -40 ° C for 48 hours using 300 g (yield yield 9.5%) (hereinafter referred to as "DH") and 3.6 kg of non-leaflets samples of Hwangchil-tree hot water extract of the present invention and 4 hours at 100 ° C Heat-extracted and concentrated under reduced pressure and freeze-dried for 48 hours at -40 ℃ using a freeze-drier (Ilsin Lab, Korea) 700 g (yield 19.4%) of the non-leaflet hot water extract of the present invention (hereinafter referred to as "EH") Each was obtained and used as a sample of an experiment example.

Example 2 Preparation of Ethanol Extracts of Hwangchil Tree and Non-leafed Leaves

2-1. Preparation of 5% Ethanol Extracts from Hwangchil Tree and Loquat Leaf

      After drying the dried Hwangchil trees and non-leaved leaves (produced in Jangheung, Jeonnam), 3.2 kg of Hwangchil wood samples were added to 36 L of 5% ethanol and extracted by heating at 100 ° C for 4 hours, and the filtrate was concentrated under reduced pressure. Lyophilized at -40 ° C. for 48 hours using 200 g (yield of 6.3%) (hereinafter referred to as “D5H”) and 3.2 kg of non-lobe sample in 36% of 5% ethanol. After heating for 4 hours at ℃ and concentrated under reduced pressure the filtrate was freeze-dried for 48 hours at -40 ℃ using a freeze dryer (Ilsin Lab, South Korea) 720 g (yield 20.0% yield) of the present invention (hereinafter "E5H" Each was used as a sample of the experimental example.

2-2. Preparation of Sacred Tree 50% Ethanol Extract

36 L of dried Hwangchil wood samples were added to heat extracted at 80 ° C for 4 hours, the filtrate was concentrated under reduced pressure, and lyophilized at -40 ° C for 48 hours using a freeze dryer. 215 g (yield 6.7%) (hereinafter referred to as "D50E") were obtained and used as a sample for the experimental example.

2-3. Preparation of Hwangchil-tree 80% Ethanol Extract

Add 36 L of 80% ethanol to the dried Hwangchil wood sample, heat extract at 80 ° C for 4 hours, concentrate the filtrate under reduced pressure, and lyophilize at -40 ° C for 48 hours using lyophilizer. 155 g (yield 4.8%) of ethanol extract (hereinafter referred to as D80E ") were obtained and used as a sample of the experimental example.

Example 3 Preparation of Mixed Extract of Hwangchil Tree and Non-leafed Leaves

Hwangchil wood and non-leaflet individual dry extracts obtained in the above examples were mixed with each other in the ratio shown in Table 1 was used as a sample in the following experimental example.

Example Number Mixing ratio denomination 3-1 DH: E5E (1: 1, w / w) 0 3-2 D5E: E5E (1: 1, w / w) 5 3-3 D50E: E5E (1: 1, w / w) 50 3-4 D80E: E5E (1: 1, w / w) 80

Experimental Example 1. Experiment of radical scavenging ability

DPPH method (Blios, MS, Antioxidant determinations by the use of a stable free radical. 395. Nature 26: 11991200 (1958)) was used to determine the electron donating transfer capacity of the extract sample of the present invention obtained in the above example. Radical scavenging activity experiment was performed as follows.

Samples obtained in the above example were dissolved in an ethanol solution at a concentration of 1 mg / ml, and then 0.02 ml of each was transferred to a 96-well plate, followed by 0.25 mM DPPH (2,2-diphenyl-1-picrylhydrazyl, D9132) dissolved in ethanol. , Sigma) 0.18 ml were added and reacted at 37 ° C. for 30 minutes, and the absorbance value was measured at 517 nm. The radical scavenging ability was calculated according to the following Equation 1, and the result was shown. The positive control group used EGCG 1 mM (E4268, Sigma) which was very excellent in antioxidant activity.

As a result, as shown in Figure 1, it was confirmed that the non-leaflet extract showed a similar effect to the positive control group EGCG excellent in radical scavenging activity compared to the Hwangchil wood extract.

Experimental Example 2. Cytotoxicity Test

MTT assay (Yun-Lian Lina, Guei-JaneWang, Chuen-Lin Huan, Yi-Chao Lee, Wei-Chen Liao, Wen-Lin Lai, Yen-Ju Lin) to test the cytotoxicity of the sample obtained in the above example , Nai-Kuei Huan ,. J. Ethnopharmacology, 122; 417-425 (2009)).

PC12 cells (rat pheochromocytoma cell, American Type Culture Collection (ATCC; Manassas, VA)) were treated with 10% fetal bovin serum (FBS, Gibco-BRL ((Gaithersburg, MD)) and 1% antimicotics / antibiotics (Gibco-BRL). The cells were cultured in RPMI-1640 medium (Gibco-BRL) containing 10 ⁴ -10 ⁶ cells / ml.

Transfer the cultured cells to a 96-well plate and incubate the cell number at 1 × 10 ^{4 per} well, and then make a 1: 1 mixture by weight of the Hwangchil-tree ethanol extracts obtained in the above example and the non-leafy 5% ethanol extract, which was 5 Dissolve 1 mg / ml in% DMSO, add 15 parts each (final concentration: 0.1 mg / ml), and react for 24 hours at 37 ° C. MTT (3-(4,5-dimethylthiazol-2 -yl) dissolved in PBS 2,5-diphenyltetrazolium bromide, M2128, Sigma) After adding 2 mg / ml of reagent 15 for each well, reacting at 37 ° C. for 90 minutes, measuring absorbance at 570 nm / 630 nm, and then calculating using Equation 2 below. It was. The control group was measured without processing any sample. Ascorbic acid (A5960, Sigma) with good antioxidant activity and low cytotoxicity was dissolved in 5% DMSO to 1 mM, and then treated with 0.1 mM.

As a result, as shown in Figure 2, 0, 5, 50 and 80 all confirmed that there is no cytotoxicity.

Experimental Example 3. Measurement of Neuronal Protective Activity

Rong et al. (Rong Ma, Nian Xiong, Chengfang Huang, Qiang Tang, Benrong Hu, Jizhou Xiang, Gang Li, Neuropharmacology, to test neuronal protective activity from beta amyloid-induced neurotoxicity of the sample obtained in the above example) 56: 10271034 (2009)) was performed as follows.

PC12 cells were cultured in RPMI-1640 medium (Gibco-BRL) containing antimicotics / antibiotics in 96-well plates and the cell number reached 1 × 10 ^{4 per} well. A mixture of% ethanol extract was dissolved in 5% DMSO at a concentration of 1 mg / ml, and then 15 parts of each were pre-incubated at 37 ° C. for 24 hours, followed by 25 β-amyloid (25-35) (A4559, Sigma) was treated for 24 hours to induce oxidative stress (oxidative stress), the neuronal protective activity was measured using the MTT assay of Experimental Example 2. The control group did not process any sample, the negative control group was treated only beta amyloid so that the final concentration of 25, the positive control group was measured by pretreatment 0.1 mM ascorbic acid.

As a result, as shown in Figure 3, due to the oxidative stress induced by the beta amyloid peptide, the cell activity was reduced to about 60%, 50% ethanol in the mixture of yellow-wood tree ethanol extract and non-lobe 5% ethanol extract The extract and the non-leaflet 5% ethanol mixed extract (50) showed the highest protective activity against the cell protective effect.

      Hereinafter, a preparation example of a composition containing an extract of the present invention will be described, but the present invention is not intended to be limited thereto but only to be described in detail.

Formulation Example 1 Preparation of Powder

Loquat 5% Ethanol Extract + Hwangchil Tree 50% Ethanol Extract 20 mg

Lactose 100 mg

Talc 10 mg

The above ingredients are mixed and filled in an airtight cloth to prepare a powder.

Formulation Example 2 Preparation of Tablet

Loquat 5% Extract + Yellow Chili 50% Ethanol Extract 10 mg

Corn starch 100 mg

Lactose 100 mg

Magnesium stearate 2 mg

After mixing the above components, tablets are prepared by tableting according to a conventional method for preparing tablets.

Formulation Example 3 Preparation of Capsule

Loquat 5% Ethanol Extract + Yellow Chili 50% Ethanol Extract 10 mg

3 mg of crystalline cellulose

Lactose 14.8 mg

Magnesium stearate 0.2 mg

According to a conventional capsule preparation method, the above ingredients are mixed and filled into gelatin capsules to prepare capsules.

Formulation Example 4 Preparation of Injection

Loquat 5% Ethanol Extract + Yellow Chili 50% Ethanol Extract 10 mg

Mannitol 180 mg

Sterile distilled water for injection 2974 mg

Na ₂ HPO _4, 12H ₂ O 26 mg

According to the conventional method for preparing an injection, the amount of the above ingredient is prepared per ampoule (2 ml).

Formulation Example 5 Preparation of Liquid

Loquat 5% Ethanol Extract + Sacred Tree 50% Ethanol Extract 20 mg

10 g of isomerized sugar

5 g of mannitol

Purified water

After dissolving each component in purified water according to the usual method of preparing a liquid solution, adding a proper amount of lemon aroma, and then mixing the above components, adding purified water and adjusting the whole to 100 ml by adding purified water and filling into a brown bottle. The solution is prepared by sterilization.

Formulation Example 6 Preparation of Healthy Food

Loquat 5% Ethanol Extract + Yellow Chili 50% Ethanol Extract 1000 mg

Vitamin mixture proper amount

70 μg of vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Formulation Example 7 Preparation of Healthy Drink

Loquat 5% Ethanol Extract + Yellow Chili 50% Ethanol Extract 1000 mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

1 is a view showing the scavenging ability of the ethanol fraction extract and non-leafy 5% ethanol extract mixture of yellow lacquer tree (0; 0% EtOH yellow lacquer extract + 5% EtOH non-leaf leaves extract; Extract, 50; 50% EtOH yellow lacquer extract + 5% EtOH non-leaf leaves extract, 80; 80% EtOH yellow lacquer extract + 5% EtOH non-leaf leaves extract, the final concentration of the sample: 100 ㎍ / ml),

2 is a diagram showing the effect on the cytotoxicity of the ethanol fraction extract and non-leafy 5% ethanol extract mixture of Hwangchil-tree (Control: non-treated; 0% EtOH yellow chile extract + 5% EtOH non-leafy extract, 5; 5% EtOH Hwangchil Tree Extract + 5% EtOH Non-leaf Leaf Extract, 50; 50% EtOH Hwangchil Extract + 5% EtOH Non-leaf Leaf Extract, 80; 80% EtOH Hwangchil Extract + 5% EtOH Non-leaf Leaf Extract, Final Concentration of Sample: 100µg / ml) Is,

3 is a diagram showing the neuronal protective activity of the ethanol fraction extract and non-leaflet 5% ethanol extract mixture of hwangchil tree from neurotoxicity caused by beta amyloid (Control: non-treated control; con-1: beta amyloid 25 treatment; 0% EtOH: 0% Ethanol Sacred Tree Extract + 5% Non-foliated Ethanol Extract, 50% EtOH: 50% Ethanol Sacred Tree Extract + 5% Non-foliated Ethanol Extract, 80% EtOH: 80% Ethanol Sacred Tree Extract + 5% Non-foliated Ethanol Extract, 5 : Beta amyloid 25 treatment (24 hr) after 5 μg / ml (salt of ethanol fraction extract + 5% ethanol non-leaf extract), 10: 10 μg / ml (salt of ethanol fraction extract + 5% ethanol non-leaf) Beta amyloid after treatment with beta amyloid 25 after treatment (24 hr), 25: 25 μg / ml extract mixture (sacred tree ethanol fraction extract + 5% ethanol non-lobe extract) 25 treatments (24 hr), 50: 50 μg / ml extract mixture (sacred tree ethanol fraction extract + 5% ethanol non-lobe extract) treatment after treatment with beta amyloid 25 (24 hr), 100: 100 μg / ml extract mixture (salt wood) Ethanol fraction extract + 5% ethanol non-lobe extract) followed by beta amyloid 25 treatment (24 hr).

Claims (9)

Treatment of Alzheimer's disease or Parkinson's disease containing as an active ingredient the water and ethanol mixed solvent soluble extracts of Hwangchil trees and non-leafs mixed with a dry weight ratio of Hwangchil trees and non-leafed leaves in a mixing ratio of 1 to 2: 1 (w / w), and Preventive pharmaceutical composition. delete delete delete delete Prevention of Alzheimer's disease or Parkinson's disease, containing as an active ingredient the water and ethanol mixed solvent soluble extracts of the yellow lacquer tree and the non-leaf leaves mixed with the dry weight ratio of the yellow lacquer and the non-leaf leaves in a mixing ratio of 1 to 2: 1 (w / w) and Health functional food for improvement. delete delete delete

Images