KR101825420B1 - Hematococcus nano-dispersed aqueous solution manufacturing method - Google Patents

Abstract

The present invention relates to a manufacturing method of a Haematococcus nano-dispersed aqueous solution, and more particularly, the present invention relates to a method for producing a Haematococcus nano-dispersed aqueous solution, which can enhance the astaxanthin efficacy and improve digestion and absorption capacity by nano-hydrating Haematococcus extract powder.

Description

[0001] The present invention relates to a hematococcus nano-dispersed aqueous solution manufacturing method,

The present invention relates to a method for preparing a hematocookus nano-dispersed aqueous solution, and more particularly, to a method for producing hematocookus nano-dispersed aqueous solution by hydrothermally hydrolyzing a hematococzus extract powder to increase the activity of astaxanthin, To a method for producing an aqueous dispersion.

As the desire to maintain beautiful and healthy skin due to the improvement of living standards has increased, interest in cosmetic functional foods, which are effective for skin cosmetic, has been increased and researches for finding various functional food materials have been actively conducted. In addition, the amount of ultraviolet rays reaching the ground surface due to environmental pollution increases year by year, and accordingly, there is an increasing interest to inhibit skin damage caused by sunlight exposure (He Kyoung Jun et al., Korean Journal of Food Preservation. 21 (2): 264-275, 2014). In modern industrialized society, factors that cause skin aging such as westernized lifestyle and stress are also increasing, and various methods to improve skin health have been studied (Yoo Seok Jeong et al., Journal of Korean Society of Food Science and Nutrition 42 (11): 1744-1752 (2013)).

The aging of the skin is divided into endogenous aging caused by decrease of hormone secretion, immune cell function and activity decrease, biosynthetic protein biosynthesis is reduced, and exogenous aging by contaminated air, drug or ultraviolet ray (Park HR et al., Journal of Investigative Cosmetology 7: 115-122, 2011). Among extrinsic aging, especially ultraviolet rays, skin wrinkle formation, elasticity reduction, pigmentation, and skin barrier damage are caused by reduced skin moisture content, which is a long-term aging phenomenon observed in sun-exposed skin. This leads to a loss of elasticity of the stratum corneum on the skin surface, resulting in dry or rough skin, which is called photoaging (Ivana Binic et al., Evidence-Based Complementary and Alternative Medicine.

When photoaging occurs, the skin layer collapses and substrate proteins such as collagen and elastic fibers are damaged. When the amount of collagen in the skin becomes insufficient and the elastic fibers are denatured, wrinkles are formed. Ultraviolet light reduces the synthesis of collagen and increases the expression of matrix metalloproteinase (MMP), an extracellular matrix protease (Jin Young Seo et al , Korean Journal of Investigative Dermatology, 8 (4): 187-194, 2001). Representative clinical features of skin aging include fine lines, dry skin, and reduced elasticity. When skin aging occurs, coarse and deep wrinkles occur, and fine wrinkles also develop. In particular, irregular pigmentation occurs in the skin exposed to the sun, resulting in increased pigmentation such as sunlight. In addition, the skin becomes very rough, dry, and elasticity is reduced, resulting in severe skin burns (Karin Scharffetter Kochanek et al., Experimental Gerontology 35 (2000): 307-316.

Currently, a variety of new materials are being explored for food compositions and cosmetic compositions for improving skin wrinkles. However, there is a merit that a combination of a natural active substance which is effective for the improvement of skin wrinkle among a standardized material or a certified health food material of a food and beverage can be developed a functional food and a cosmetic which are stable and safe compared to the existing one In addition, it is possible to develop a food composition and a cosmetic composition for improving skin wrinkles, which exhibit an enhanced effect over individual components having a wrinkle-improving effect of the prior art.

As a research for improving the skin wrinkles, Korean Patent Registration No. 10-1613546 discloses a composition comprising soybean extract and Haematococcus pluvialis extract at a weight ratio of 1: 0.1 to 10 A composition for food for preventing or improving skin wrinkles is disclosed.

Here, astaxanthin, an extract component of Hematococcus, has a strong antioxidant ability called super vitamin E, which increases the blood flow of the retina, thereby improving eye fatigue and improving wrinkles.

It is 3 times stronger than known lutein, and it is lipid-soluble substance, so it has affinity for cell membrane and is very good for eye health and cell function.

It has superior antioxidant activity in fat structure than vitamin E.

Astaxathin (3,3'-dihydroxy-β, β-carotene-4,4'-dion e), a compound of the formula C ₄ 0H ₅₂ O ₄ , is a vitamin A precursor, widely distributed in nature, (Di Mascio, P. et al. (1991) Am. J. Clin. Nutr., 53: 194S-200S), belonging to the carotenoid family of xanthophyll, In fact, astaxanthin, a scarlet or bright orange pigment present in many foods consumed by humans, is naturally produced and is the most prevalent in marine and marine animals, especially in shrimp, red seabream, salmon ), And lobster (Fujita, et al. (1983) Nippon Suisan Gakkaishi., 49: , 11: 297-326; Nelis, et al. (1991) J. Appl. Bacteriol., 70: 181-191). In addition, redness is caused by the decomposition of the pigment protein and the appearance of red astaxanthin. Since it can not be synthesized directly in vivo, it must be ingested from food or mouth.

Also, because our body has a water content of about 70%, the water-soluble material is better absorbed than the lipid-soluble material.

In order to maximize the effect of astaxanthin, an extract component of liposomal hematococcus, development of water-soluble astaxanthin and development of processed foods containing it have been required.

Korean Patent No. 10-1613546 Korean Patent Publication No. 10-2005-0116455 Korean Patent Publication No. 10-2017-0063338 Korean Patent Publication No. 10-2009-0087183

In order to solve the above-mentioned problems, the present invention provides a method for preparing aqueous solution of hematococzus nano-dispersion, which is capable of doubling astaxanthin efficacy and enhancing digestion and absorption capacity by hydrolyzing the hematococcus extract powder with nano.

The problems to be solved by the present invention are not limited to those mentioned above, and other solutions not mentioned can be clearly understood by those skilled in the art from the following description.

As a means for achieving the object of the present invention, the method for preparing a hematocookus nano-dispersed aqueous solution according to the present invention comprises a step S1 of preparing a hematococzus extract powder by pulverizing and extracting a dried culture product of hematococcus Wow;
Preparing a pretreatment solution comprising 20 to 30 parts by weight of the extracted hematococzus powder, 60 to 80 parts by weight of purified water, and 0.1 to 5 parts by weight of a natural emulsifier;
30 to 60 parts by weight of the pretreatment solution, 30 to 60 parts by weight of purified water, and 1 to 5 parts by weight of a dispersion stabilizer, and pulverizing the hematococzus extract powder to nanosize ,
In step S1, culturing of the hematococzus is performed by alternately supplying oxygen and carbon dioxide into the incubation chamber to control the switching between the exhalation state and the anaerobic state. An infrared lamp is installed in the incubation chamber, And is cultured under control so that infrared irradiation is not performed in the anaerobic state.

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Further, in the method for producing an aqueous solution of hematocookus nano-dispersion according to the present invention, at least one of lecithin, glycerin, polysorbate, fatty acid ester, gadji gum, gum arabic, xanthan gum and guar gum is selected as the natural emulsifier , And the dispersion stabilizer is characterized in that at least one of galactose, arabinose, galacturonic acid, rhamnose, xylose, fucose and glucose is selected.

The present invention has the effect of doubling the astaxanthin efficacy and enhancing the digestion and absorption capacity by making the hematococcus extract powder nano-hydrate by the method of producing a hematocookus nano-dispersed aqueous solution.

The effects of the present invention are not limited to those mentioned above, and other solutions not mentioned may be clearly understood by those skilled in the art from the following description.

Fig. 1 is a block diagram showing each step of the method for producing a hematocookus nano-disperse aqueous solution according to the present invention.
Fig. 2 is a photograph of an aqueous solution of a hematocook dispersion in Example 1; Fig.
Fig. 3 is a photograph of an aqueous solution of a hematocookus nano-dispersion of Example 1; Fig.
4 is data showing particle size distribution of a hematocookus nano-dispersed aqueous solution.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings and the following description. However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Rather, the embodiments disclosed herein are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

Fig. 1 is a block diagram showing each step of the method for producing a hematocookus nano-disperse aqueous solution according to the present invention.

A method for preparing a hematocookus nano-dispersed aqueous solution according to the present invention comprises the steps of: preparing a hematococzus extract powder; preparing S2, which comprises a pretreatment solution obtained by mixing the hematococz extract powder, purified water and a natural emulsifier; And preparing a dispersion solution containing the pretreatment solution, purified water, and a dispersion stabilizer, and pulverizing the hematococzus extract powder to have a nanosize size.

The hematococz extract powder obtained in the step S1 is obtained by pulverizing and extracting the dried material cultured in the hematococcus.

Hematococcus pluvialis is a unicellular green algae that is one of the photosynthetic microorganisms. It has two major types depending on the external growth environment: green (green) vegetative cells and red cyst cells with no mobility. Green cells grow steadily in a favorable environment, and when they are exposed to unfavorable environment caused by various changes in external environment conditions, the green cells are converted into red cyst cells in accordance with the self-protection mechanism, It accumulates a large amount of tarangin. In order to utilize these features, the present invention is characterized in that hematococzus forms a condition capable of accumulating astaxanthin in a red dormant phase during the culturing process.

As the culturing method of the step S1, a large amount of astaxanthin can be accumulated in the anaerobic state by alternating the anaerobic state and the exhalation state when the hematococzus is cultured in the medium.

Herein, the medium may be a known liquid growth medium containing Amino acids, Inorganic salts (calcium chloride, potassium sulfate, sodium chloride, monosodium phosphate), Glucose vitamins (folic acid, nioctinamide, riboflavin, B12) and the like.

When the medium is put in a culture chamber and incubated, it can be confirmed through an experiment that oxygen and carbon dioxide are alternately supplied. An oxygenated state is formed by reducing the specific gravity of oxygen in the culture chamber by injecting carbon dioxide, The level of protection against human deaths should be maintained.

An infrared lamp may be installed in the incubation chamber to irradiate the medium with infrared rays in the aerobic state to maintain the beneficial state of the cells and to induce the cell activity in the anaerobic state without infrared irradiation. On the other hand, when irradiated with ultraviolet rays or irradiated with strong light, it is possible to create a harsh environment because the hematococcus grows as compared with the infrared irradiation. However, since there is a risk of cell death or the like, Is adopted.

Here, the exhalation state and the anaerobic state period are 1: 3 to 5.

In general, the accumulation of astaxanthin in the red dormant phase is at most 3%, and when controlling aerobic / anaerobic conditions and infrared irradiation, the astaxanthin accumulation can be increased to 4.5%.

On the other hand, known extraction methods such as extraction using carbon dioxide or the like (supercritical extraction) or extraction with acetone can be exemplified.

Step S2 is a step of preparing a pretreatment solution comprising a hematococz extract powder, purified water, and a natural emulsifier. The pretreatment solution is prepared by mixing 20 to 30 parts by weight of a hematococz extract powder, 60 to 80 parts by weight of purified water And 0.1 to 5 parts by weight of a natural emulsifier.

Herein, the natural emulsifier may be selected from at least one selected from lecithin, glycerin, polysorbate, fatty acid ester, gadji gum, gum arabic, xanthan gum and guar gum, but the present invention is not limited thereto.

In the step S3, a dispersion solution obtained by mixing the pretreatment solution of step S2, purified water, and a dispersion stabilizer is prepared and the hematococz extract powder is pulverized to have a nano size. The dispersion solution contains 30 to 60 parts by weight of the pretreatment solution 30 to 60 parts by weight of purified water, and 1 to 5 parts by weight of a dispersion stabilizer.

The dispersion stabilizer is used for preventing recombination because the extracted powder of hematocookus pulverized into nano-sized particles is recombined and increased again. In order to prevent recombination, at least either of galactose, arabinose, galacturonic acid, rhamnose, xylose, fucose, But the present invention is not limited thereto.

And the reason for crushing by nano size is to increase the digestion capacity of astaxanthan.

The aqueous solution of hematocookus nano-dispersion which has undergone the above step S3 can be used as a food additive such as a beverage or as an additive for a functional cosmetic additive.

In the case of the food additive, at least one of white sugar, citric acid, vitamin C, xanthan gum, fragrance, pigment, and foam cream is selected, but the present invention is not limited thereto.

After sterilization and sealing packaging, it is refrigerated.

Hereinafter, a more preferred embodiment of the method for preparing a hematocookus nano-dispersed aqueous solution according to the present invention will be described.

1. Step S1

Prepare a hematococcus extract powder.

Here, the raw material of the extracted powder of Hematococcus was ALGAEBIOTECH (the Netherlands).

Step S2

20 parts by weight of extracted hematocook powder, 78 parts by weight of purified water, and 1 part by weight of lecithin is prepared.

Step S3

50 parts by weight of the pretreatment solution, 48 parts by weight of purified water and 2 parts by weight of galactose were added to the nanomill mill shown in FIG. 2 and pulverized to obtain an aqueous solution of the hematococcus nanodispersion of FIG. Fig. 4 shows the particle size distribution of the hematocookus nano-dispersed aqueous solution. The particle size was 464 nm.

Next, 3 parts by weight of a hematocookus nano-dispersed aqueous solution and 10 parts by weight of white sugar, 0.11 part by weight of citric acid, 0.2 parts by weight of vitamin C and 0.1 part by weight of xanthan gum were added to 100 parts by weight of purified water based on 100 parts by weight of purified water, Seal it in a container.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, is intended to cover various modifications and similarities. Accordingly, the scope of the present invention should be construed as being limited to the embodiments described, and it is intended that the scope of the present invention encompasses not only the following claims, but also equivalents thereto.

Claims (3)

A step S1 of crushing and extracting a dried product cultured with hematococzus to prepare a hematococz extract powder;
Preparing a pretreatment solution comprising 20 to 30 parts by weight of the extracted hematococzus powder, 60 to 80 parts by weight of purified water, and 0.1 to 5 parts by weight of a natural emulsifier;
30 to 60 parts by weight of the pretreatment solution, 30 to 60 parts by weight of purified water, and 1 to 5 parts by weight of a dispersion stabilizer, and pulverizing the hematococzus extract powder to nanosize ,
In step S1, culturing of the hematococzus is performed by alternately supplying oxygen and carbon dioxide into the incubation chamber to control the switching between the exhalation state and the anaerobic state. An infrared lamp is installed in the incubation chamber, Wherein the culturing is performed while controlling the irradiation of infrared light in an anaerobic state.
delete The method according to claim 1,
Wherein the natural emulsifier is selected from at least one of lecithin, glycerin, polysorbate, fatty acid ester, gadji gum, gum arabic, xanthan gum and guar gum,
Wherein the dispersion stabilizer is at least one selected from the group consisting of galactose, arabinose, galacturonic acid, rhamnose, xylose, fucose and glucose.

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