KR101823745B1 - A saliva module for collecting and keeping and preprocessing for it - Google Patents

Abstract

The present invention relates to a saliva collecting module capable of collecting, storing and pretreating saliva, and more particularly, to a saliva collecting module capable of collecting, storing, and pretreating saliva at a time from saliva collection to storage and without a centrifuge A main body unit (100) in which saliva is received; A door unit 200 coupled to the main unit 100 to seal the main unit 100; A saliva discharging regulating unit 300 connected to the main body unit 100 and discharging the saliva contained in the main body unit 100 to the outside of the main body unit 100 according to the amount of rotation; Including the saliva collection module capable of collecting, storing, and pretreating saliva, which is capable of collecting, storing, and pretreating saliva containing saliva, which is similar to that of a centrifugal separator.

Description

[0001] The present invention relates to a saliva collection module for collecting, storing, and pretreating saliva,

The present invention relates to a saliva collecting module capable of collecting, storing and pretreating saliva, and more particularly, to a saliva collecting module capable of collecting, storing, and pretreating saliva at a time from saliva collection to storage and without a centrifuge will be.

Saliva is a colorless, sticky substance that is released from the inside of the mouth. It is a digestive juice that acts to convert starch to maltose or maltose to glucose.

On the other hand, pepsin is a digestive enzyme secreted from stomach main cells.

Pepsin is detected in saliva in cases such as reflux phos- phitis, which means that gastric juice is detected in the oral cavity.

Therefore, when pepsin is contained in saliva in gastric juice, reflux esophagitis and the like can be suspected.

Saliva contains molecules such as proteins and nucleic acids that are sufficient to reflect the pathology and physiological conditions of the body, such as blood, urine, and cerebrospinal fluid, and saliva collection is safe and easy and noninvasive for both the examinee and the subject It can be recognized as a highly utilized disease diagnosis technology.

Therefore, diagnostic kits using non-invasive saliva have been developed to diagnose diseases such as reflux pharyngitis.

However, the conventional diagnostic kit for detecting pepsin was carried out through a pipette test.

The pipette test had to be provided with a separate instrument and container for collecting saliva and for pretreatment to remove impurities other than pepsin in the saliva.

Conventional saliva diagnosis using a pipette test has inconvenienced that saliva collection and sample treatment must be performed in several steps.

In addition, it is not only complicated to take a certain amount of saliva sampled and reintroduced, but also requires centrifugation to be carried out directly. Therefore, it is not only inconvenient to use by the general public, but also has a problem in that the precision of pepsin measurement is greatly deteriorated.

Conventionally, the centrifugal separator has been used to limit the pre-treatment of the saliva, and even the pretreatment device has a complicated structure.

(Patent Document 1) US2014-0302617 A1

(Patent Document 2) US5393496 A

(Patent Document 3) US9113850 B2

Accordingly, it is an object of the present invention to provide a saliva collecting module capable of collecting, storing and pretreating saliva having an accuracy equivalent to that of using a centrifuge without using a centrifuge The purpose is to do.

In order to solve the above-mentioned problems, the present invention provides a medical device comprising: a main body unit (100) for receiving saliva; A door unit 200 coupled to the main unit 100 to seal the main unit 100; A saliva discharging regulating unit 300 connected to the main body unit 100 and discharging the saliva contained in the main body unit 100 to the outside of the main body unit 100 according to the amount of rotation; And a saliva collection module capable of collecting, storing and pretreating saliva.

Meanwhile, the saliva control unit 300 includes a grip unit 310 coupled to the joint unit 130 formed in the main body unit 100; A nozzle unit (320) extending from the grip unit (310) and communicating with the main unit (100) to provide a discharge path of the saliva in the main unit (100); The main unit 100 is closed when the grip unit 310 is rotated in one direction and the main unit 100 is opened when the grip unit 310 is rotated in the other direction, A packing unit 330; A saliva collection module capable of collecting, storing, and pretreating saliva containing the saliva.

Further, the packing unit 330 includes a protrusion unit 336 for sealing the discharge port 140 formed at the end of the main body unit 100; A through hole that is formed adjacent to the protrusion unit 336 and provides a flow path for the discharge port 140 and the nozzle unit 320 to communicate when the protrusion unit 336 is separated from the discharge port 140; And a saliva collection module capable of collecting, storing and pretreating saliva.

The packing unit 330 is formed in a disk shape as a whole and has a round surface 337 which is shifted from the outer circumferential surface toward the central portion 335 in the direction of the nozzle unit 320, A collection module may also be provided.

The through holes may include a plurality of through holes at the same distance from the central portion 335, and may include a saliva collecting module capable of collecting, storing, and pretreating saliva opened in the longitudinal direction along the circumferential direction.

Further, the door unit 200 includes a handle unit 210; An axle unit 220 extending from the handle unit 210; A sealing unit 230 formed at an end of the axle unit 220 to seal the main body unit 100; The present invention can provide a saliva collection module capable of collecting, storing, and pretreating saliva.

Also, the body unit 100 may be provided with a saliva collection module capable of collecting, storing, and pretreating saliva provided with citric acid as a preservative.

In addition, the saliva discharging regulation unit 300 may include a saliva collecting module capable of collecting, storing, and pretreating a saliva capable of installing a polypropylene filter (PP filter) as a pretreatment filter.

The present invention as described above has the following effects.

First, there is a strong point that the pretreatment efficiency is the same as that of using a centrifugal separator without using a centrifugal separator.

Second, since each of the parts can be a separable structure, there is an advantage that it is easy to clean and reuse.

Third, there is an advantage of providing a pepsin detection device which can distinguish between a normal person and a patient.

Fourth, there is a strong point in securing the stability of pepsin in preserving saliva.

Fifth, since the PP filter is used, the concentration of pepsin after passing through the pretreatment filter is minimized.

Seventh, the centers of the first through holes 331, the second through holes 332, the third through holes 333, the fourth through holes 334, and the nozzle unit 320 are not aligned with each other. Therefore, since the saliva passes directly through the first through-hole 331, the second through-hole 332, the third through-hole 333, and the fourth through-hole 334 without going straight to the nozzle unit 120, So that the discharge can be performed.

1 is an overall perspective view according to a first preferred embodiment of the present invention.
2 is an exploded perspective view according to a first preferred embodiment of the present invention.
3 is an overall perspective view according to a second preferred embodiment of the present invention.
4 is an exploded perspective view according to a second preferred embodiment of the present invention.
FIG. 5 is a graph illustrating the safety of pepsin according to the storage time according to the storage time according to the preferred embodiment of the present invention.
FIG. 6 is a graph showing a result of evaluating changes in pepsin concentration before and after passage of a filter according to a filter type according to a preferred embodiment of the present invention.
FIG. 7 is a graph showing a comparison of pepsin concentration values using a centrifugal separator using samples of 10 patients according to a preferred embodiment of the present invention and using only saliva collection module capable of collecting, storing, and pretreating saliva of the present invention The result is a graph.
FIG. 8 is a graph showing the results of pepsin concentration values before and after passage through a filter using samples of a normal group and a patient group, respectively, according to a preferred embodiment of the present invention.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

In this process, the thicknesses of the lines and the sizes of the components shown in the drawings may be exaggerated for clarity and convenience of explanation. In addition, the terms described below are defined in consideration of the functions of the present invention, which may vary depending on the intention or custom of the user, the operator. Therefore, the definitions of these terms should be described based on the contents throughout this specification.

≪ Description of main body unit 100 >

The main body unit 100 provides a reservoir for receiving saliva.

The main body unit 100 has a reservoir unit 120 in which saliva is stored largely. An expanding unit 110 is formed at one end of the reservoir unit 120, and at the other end of the reservoir unit 120, A joint unit 130 is formed.

The reservoir unit 120 is provided with a measurement graduation so that the amount of saliva stored on the side wall can be measured.

The discharge port 140 is formed at an end of the reservoir unit 120 where the joint unit 130 is formed and has a diameter smaller than the diameter of the reservoir unit 120. [

The expanding unit 110 is formed in such a manner that the diameter of the end surface gradually increases toward the direction away from the reservoir unit 120. [ Therefore, the saliva can be provided more stably.

Although the expanding unit 110 according to the preferred embodiment of the present invention is provided integrally with the reservoir unit 120, in another embodiment, the expanding unit 110 may be a detachable structure with the reservoir unit 120 Leave.

The joint unit 130 according to the preferred embodiment of the present invention may be threaded on the outer peripheral surface of the end portion of the reservoir unit 120.

However, it is also possible that the joint unit 130 according to another embodiment of the present invention is formed in a structure that can be detached and removed in a one-touch manner as a clapping device (not shown).

< door  Explanation of Unit 200>

The door unit 200 is coupled to the main body unit 100 to maintain the airtightness of the main unit 100.

More specifically, the door unit 200 is largely formed of a handle unit 210, an axle unit 220, and a sealing unit 230.

The handle unit 210 is an operating part for gripping the door unit 200, and is formed in a disc shape as a whole.

A plurality of protrusions may be provided on the outer circumferential surface of the handle unit 210 to prevent slippage in the rotating operation.

The axle unit 220 is formed as an axis extending from the handle unit 210.

The sealing unit 230 is formed at the end of the axle unit 220 with the overall width being equal to or greater than the inner diameter of the reservoir unit 120 so that the reservoir unit 120 can be sealed.

The sealing unit 230 may be formed of a rubber material such as urethane.

More specifically, the sealing unit 230 may be formed so that the cross-sectional area gradually decreases in the direction away from the axle unit 220, and it may be preferable that the sealing unit 230 is formed as a circular rod having a convexly rounded shape as a whole .

It is noted that the sealing unit 230 may be of a structure that can be detached from the axle unit 220.

<Saliva Discharge  Description of Control Unit 300>

The saliva discharging control unit 300 includes a grip unit 310, a nozzle unit 320, and a packing unit 330.

The saliva discharging control unit 300 is connected to the main body unit 100 and discharges the saliva discharged to the outside of the main body unit 100 by varying the amount of the saliva contained in the main body unit 100 according to the amount of rotation of the saliva discharging control unit 300 It is possible.

In other words, the saliva dispensing regulating unit 300 according to the first preferred embodiment of the present invention is a structure that can be attached to and detached from the main body unit 100.

More specifically, the grip unit 310 is engaged with the joint unit 130 formed in the main body unit 100.

The grip unit 310 forms a thread to be coupled with the joint unit 130 on the inner circumferential surface while forming the overall contour of the saliva dispensing control unit 300.

The grip unit 310 is formed with a plurality of projections on its outer circumferential surface to facilitate gripping and rotation.

The nozzle unit 320 extends from the handle unit 210 and communicates with the main unit 100 to provide a path of saliva inside the main unit 100. [

The nozzle unit 320 is narrowly and narrowly extended from one end face of the grip unit 310.

The nozzle unit 320 provides a narrow fluid communication passage for fluid communication with the interior of the grip unit 310.

The packing unit 330 is provided inside the grip unit 310.

The packing unit 330 can seal the main unit 100 when the grip unit 310 rotates in one direction and can open the main unit 100 when the grip unit 310 rotates in the other direction.

More specifically, when the saliva discharging control unit 300 is rotated in one direction and is completely fastened to the joint unit 130, the packing unit 330 can seal the main unit 100.

On the contrary, when the saliva discharging regulation unit 300 is rotated in the other direction and is not completely fastened from the joint unit 130, the packing unit 330 can open the main unit 100.

When the packing unit 330 inserts and presses the piston into the main body unit 100 while the main unit 100 is opened, the saliva in the reservoir unit 120 can be discharged through the nozzle unit 320.

According to the first preferred embodiment of the present invention, the saliva discharging regulation unit 300 can be completely separated from the main body unit 100 when the saliva discharging regulation unit 300 is continuously rotated in the other direction.

The packing unit 330 has a protruding unit 336 and a through-hole.

The through hole may include a first through hole 331, a second through hole 332, a third through hole 333, and a fourth through hole 334, and the number of the through holes may be increased or decreased as much as possible .

The protrusion unit 336 seals the discharge port 140 formed at the end of the main body unit 100 when the saliva discharging control unit 300 is fastened to the main body unit 100 to prevent the saliva contained in the reservoir unit 120 Leakage can be suppressed.

The through hole may be formed adjacent to the protrusion unit 336 and may provide a flow path so that the discharge port 140 and the nozzle unit 320 communicate with each other when the protrusion unit 336 is separated from the discharge port 140. [

At this time, the flow rate of the saliva discharged through the nozzle unit 320 can be adjusted according to the degree of opening of the discharge port 140 by the projection unit 336 according to the amount of rotation of the saliva discharge control unit 300.

More specifically, the packing unit 330 is formed in a disc shape as a whole.

The packing unit 330 has a rounded surface 337 that is offset toward the nozzle unit 320 toward the central portion 335 from the outer peripheral surface.

In other words, the packing unit 330 has a center portion 335 at its center, and a protrusion unit 336 is protruded from the center portion 335 on one side of the packing unit 330.

The protrusion unit 336 is formed to seal or open the discharge port 140 when the saliva discharging regulation unit 300 is fastened to the main body unit 100.

The plurality of through holes may be formed at the same distance from the center portion 335, respectively.

More specifically, the first through-hole 331, the second through-hole 332, the third through-hole 333, and the fourth through-hole 334 may be formed at equal intervals with respect to the center portion 335 have.

The first through hole 331 and the third through hole 333 are opposed to each other with the center portion 335 as the center and the second through hole 332 and the fourth through hole 334 Respectively.

Each of the plurality of through holes may be elongated in the longitudinal direction along the circumferential direction of the packing unit 330.

The central angle of each of the plurality of through holes may be the same.

The saliva in the reel jaw unit 120 passes through the first through hole 331, the second through hole 332, the third through hole 333 and the fourth through hole 334 of the packing unit 330, Unit 320. &lt; / RTI &gt;

On the other hand, the centers of the first through holes 331, the second through holes 332, the third through holes 333, the fourth through holes 334, and the nozzle unit 320 are not aligned with each other. Therefore, since the saliva passes directly through the first through-hole 331, the second through-hole 332, the third through-hole 333, and the fourth through-hole 334 without going straight to the nozzle unit 120, So that the discharge can be performed.

Next, a saliva sampling module capable of collecting, storing and pretreating saliva according to a second preferred embodiment of the present invention will be described.

The door unit 200 has the same structure as that of the first embodiment described above.

Meanwhile, the saliva discharging regulation unit 400 according to the second preferred embodiment of the present invention may be detachably coupled to the main body unit 100 or may be integrally formed.

The reservoir unit 120 has a measurement graduation for measuring the volume of the saliva on the side surface.

The saliva dispensing regulating unit 400 may include a housing unit 410 coupled to the joint unit 130.

The filter unit 120 may be formed of a membrane filter.

The housing unit 410 is formed with a seating surface on which a membrane filter is installed.

In the housing unit 410, a hole through which the saliva that has passed through the membrane filter is discharged is formed on the seating surface.

The filter casing unit 420 is coupled to the housing unit 410 to fix the membrane filter installed in the housing unit 410.

The packing unit 430 of the second embodiment can also be similarly described in the structure and the shape of the packing unit 330 of the first embodiment described above, so that description thereof is omitted.

On the other hand, it should be understood that in the first embodiment described above, a pre-processing filter unit, which is not shown in the pre-processing filter but connected to the nozzle unit 320, can be provided.

<Explanation of experimental graph>

Next, experimental results using a saliva collecting module capable of collecting, storing, and pretreating saliva according to preferred embodiments of the present invention will be described.

5 shows the result of performing a safety evaluation of pepsin by storage time according to the kind of the preservative liquid.

According to FIG. 5, 0.1 M of citric acid is suitable as a saliva preservative solution, and it shows the best stability in citric acid immediately after storage or after 6 hours.

6 shows the results of measurement of the change in the concentration of pepsin before and after passage of the filter depending on the type of the filter.

According to Fig. 6, PP filter shows a favorable effect of preserving pepsin in the kind of filter.

In the case of using a PVDF filter, it can be seen that pepsin after passing through the filter is hardly detected, which is not preferable.

On the other hand, in the case of using the PP filter, it can be seen that the amount of pepsin preserved after passing through the filter was remarkably increased as compared with the case of using the PVDF filter.

FIG. 7 is a graph showing the relationship between the amount of saliva collected by the conventional method and the amount of the saliva collected by the saliva collecting module of the present invention without using a centrifugal separator. The concentration of pepsin is shown in contrast.

According to FIG. 7, even if only the saliva collection module capable of collecting, storing and pretreating the saliva of the preferred embodiments of the present invention is used, the results are almost the same as those obtained by using a centrifuge.

In other words, if the saliva collection module capable of collecting, storing and pretreating saliva according to the preferred embodiment of the present invention is used, accurate pepsin detection can be performed without using a centrifuge.

FIG. 8 shows the results of comparing the concentration values of pepsin measured after passing through a selected PP filter using samples of normal (blue point) and patient (red point), respectively.

In the case of normal persons, it is preferable that no pepsin is detected at all.

According to FIG. 8, when the saliva collecting module capable of collecting, storing and pretreating saliva according to the preferred embodiments of the present invention is used, the amount of pepsin detected in a normal person is zero.

Therefore, when the saliva collecting module capable of collecting, storing and pretreating the saliva according to the preferred embodiments of the present invention is used, the distinction between a normal person and an abnormal person (patient) is made clear.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims. It will be understood that the present invention can be changed.

100: main body unit
110: expansion unit
120: Reservoir unit
130: Joint unit
140:
200: Door unit
210: Handle unit
220: axle unit
230: Sealing unit
300, 400: Saliva dispensing control unit
310: grip unit
320, 421: nozzle unit
330, 430: Packing unit
331, 431: first through hole
332, 432: second through hole
333, 433: Third through hole
334, 434: Fourth through hole
335, 435:
336, 436:
337, 437: Round face
410: housing unit
420: Filter casing unit
430: Packing unit

Claims (8)

A main body unit (100) in which saliva is received;
A door unit 200 coupled to the main unit 100 to seal the main unit 100;
A saliva discharging control unit 300 connected to the main body unit 100 so as to be rotatable and discharging the saliva stored in the main body unit 100 to the outside of the main body unit 100 according to the amount of rotation; / RTI &gt;
The saliva dispensing regulating unit 300 includes:
A grip unit 310 coupled to the joint unit 130 formed in the main body unit 100;
A nozzle unit 320 extending from the grip unit 310 and communicating with the main unit 100 to provide a discharge path of the saliva in the main unit 100; And
The main body unit 100 may be closed when the grip unit 310 rotates in one direction of the grip unit 310 and the main body unit 100 may be closed when the grip unit 310 rotates in the other direction of the grip unit 310. [ And a packing unit (330)
A saliva collection module capable of collecting, storing and pretreating saliva.
delete The method according to claim 1,
The packing unit (330)
A protrusion unit (336) for sealing the discharge port (140) formed at the end of the main body unit (100);
A through hole that is formed adjacent to the protrusion unit 336 to provide a flow path for communicating the discharge port 140 and the nozzle unit 320 when the protrusion unit 336 is separated from the discharge port 140; / RTI &gt;
A saliva collection module capable of collecting, storing and pretreating saliva.
The method of claim 3,
The packing unit (330)
And has a rounded surface 337 which is inclined in the direction of the nozzle unit 320 toward the center portion 335 from the outer circumferential surface,
A saliva collection module capable of collecting, storing and pretreating saliva.
5. The method of claim 4,
The through-
A plurality of holes are formed at the same distance from the central portion 335,
Which is opened in the longitudinal direction along the circumferential direction,
A saliva collection module capable of collecting, storing and pretreating saliva.
The method according to claim 1,
The door unit (200)
A handle unit (210);
An axle unit 220 extending from the handle unit 210; And
A sealing unit 230 formed at an end of the axle unit 220 to seal the main body unit 100; / RTI &gt;
A saliva collection module capable of collecting, storing and pretreating saliva.
The method according to claim 1,
The main body unit 100 is provided with citric acid as a preservative liquid,
A saliva collection module capable of collecting, storing and pretreating saliva.
The method according to claim 1,
The saliva discharging regulation unit 300 may be equipped with a polypropylene filter (PP filter) as a pretreatment filter,
A saliva collection module capable of collecting, storing and pretreating saliva.

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