KR101815225B1 - Nanoparticles that encapsulated with anti-wrinkles active ingredient, preparation method thereof and anti-wrinkles cosmetic composition - Google Patents

Abstract

The present invention relates to a nanoparticle encapsulating an active substance for improving wrinkles, a process for producing the nanoparticle, and a cosmetic composition for improving wrinkles containing the same as an active ingredient. The nanoparticle encapsulating the active substance for improving wrinkles according to the present invention comprises a skin permeation accelerator The present invention relates to a cosmetic composition for improving wrinkles, which is excellent in stability since the wrinkle-reducing effective substance is solved the problem of low solubility in water to provide excellent long-term stability and can further contain an antioxidant, Can be used.

Description

TECHNICAL FIELD The present invention relates to a nanoparticle encapsulated with an effective wrinkle-improving substance, a method for producing the nanoparticle, and a cosmetic composition for improving wrinkles containing the same as an active ingredient.

The present invention relates to a nanoparticle encapsulating an effective substance for improving wrinkles, a process for producing the same, and a cosmetic composition for improving wrinkles containing the same as an active ingredient.

Skin aging occurs through two main processes. The change that occurs with age without any special environmental factors is called intrinsic aging, and the change in appearance on the face, neck, and hands due to long exposure to environmental factors such as ultraviolet light It is called photo aging or extrinsic aging.

The main cause of endogenous aging is the accumulation of damage to the constituents of our body caused by reactive oxygen radicals produced during the metabolic process of our body. On the other hand, extrinsic aging is different in that harmful free radicals are produced by ultraviolet rays. Once active oxygen is produced, failure to effectively remove by various protective devices in the body results in a series of inflammatory reactions leading to skin damage, and typical damage to the skin is wrinkled skin.

The main mechanism of skin wrinkle formation is that the skin is continuously exposed to oxidative stress from harmful environments such as air pollution, ultraviolet ray exposure, stress or disease, and the radicals in the body are increased, and collagen ), Elastin, hyaluronic acid (hyaluronic acid, etc.) and can cause the subsidence phenomenon (wrinkles) in certain areas of the skin. In addition, these mechanisms oxidize the lipid part of the cell membrane and cause cell destruction, which can lead to diseases such as dermatitis, acne or skin cancer. In addition, the radical participates in the process of melanin formation, causing spots, freckles and wrinkles.

In addition, collagenase, which is a collagenase, decreases the amount of collagen production and collagen content and has the greatest effect on the wrinkles of the skin. Particularly, in the case of photo aging due to ultraviolet rays, the collagenase is overexpressed rather than natural aging.

Collagen is a macromolecular substance in the dermal layer of the skin and is known as one of the main ingredients that give skin elasticity. Therefore, efforts have been made to find a substance capable of promoting collagen biosynthesis and simultaneously inhibiting collagenase activity by a method of improving skin wrinkles.

As far as we have been studying so far, the gene that has the greatest effect on the expression of collagenase is c-Jun, a proto-oncogene. The c-Jun, together with another proto-oncogene, c-Fos, constitutes the transcription factor AP-1. The c-Jun gene is a gene that is constantly expressed in the cell, and c-Jun is a gene that increases expression by stimulation such as ultraviolet rays. The expressed c-Jun and c-Fos bind to each other to form AP-1 Thereby promoting the expression of various genes including collagenase.

Since collagenase is a major factor in the process of wrinkling, studies have been actively conducted to develop a natural extract that inhibits the activity of collagenase as an effective substance for improving wrinkles.

As a result of this, Patent Document 1 discloses an antisense oligonucleotide for collagenase gene mRNA having an excellent wrinkle-reducing effect by inhibiting collagen degradation, and a wrinkle-improving antisense oligonucleotide including an antisense oligonucleotide for the mRNA of the c-Jun gene The contents of the active substance are disclosed.

In order to improve the wrinkles of the skin, ascorbic acid, alpha -tocopherol, retinol and derivatives thereof, superoxide dismutase (SOD) and the like are used for free radical scavenging function or skin wrinkle improvement. They have been used for preventing wrinkles and other skin diseases, but they have poor long-term stability and are hard to expect a substantial effect. For this reason, the development of a safer and more effective substance for improving skin wrinkles has become an important task not only in pharmaceuticals and foodstuffs but also in the cosmetics industry, and many studies have been conducted.

Herein, retinol is a typical compound of fat-soluble vitamin A. It is known that skin epidermal cells play an important role in maintaining original function. Retinol quickly penetrates the skin and activates genes that are involved in cell growth and differentiation from the cell nucleus DNA of skin cells. In addition, by activating mRNA involved in the synthesis of proteins (collagen, etc.), collagen biosynthesis is promoted and the connective tissue of the skin is increased. It is known that the skin elasticity is improved.

However, since retinol is highly effective, it is very unstable in light, temperature, oxygen, and is easily oxidized and discolored. Even if it is applied to skin, retinol stability and effect can not be guaranteed until reaching the inside of skin. And a variety of studies have been conducted for effective retinol with long-term stability.

In addition, it is known that natural substances derived from the larvae, pupa, and cocoon of silkworms are also effective for improving wrinkles such as ascorbic acid, alpha -tocopherol, retinol and the like. For example, Patent Document 2 discloses a cosmetic composition comprising a powder, an extract or a hydrolyzate of a golden silkworm-derived substance as an effective substance. The silkworm-derived material has excellent wrinkle-improving, whitening and moisturizing effects, It has been confirmed that it is easy to formulate lotion, essence, lotion, cream, pack, gel, ointment, patch, mask sheet, spraying agent, cleaning agent and the like because of its excellent bonding force with cosmetic material.

Accordingly, the inventors of the present invention have been studying to develop nanoparticles having excellent long-term stability by encapsulating retinol, which is well-known as a wrinkle improving active material, for example, a wrinkle improving active ingredient, The nanoparticles contain a skin penetration enhancer to solve the low solubility problem of wrinkle-effective substances in water, thereby providing excellent long-term stability, and can further contain an antioxidant, thereby suppressing the oxidation of the wrinkle- Can be useful as an excellent cosmetic composition for improving wrinkles, and the present invention has been completed.

Korean Patent Publication No. 10-2006-0065813 Korean Patent Publication No. 10-2013-0108805

An object of the present invention is to provide a nanoparticle encapsulating an effective wrinkle-improving substance having excellent long-term stability and skin permeability.

Another object of the present invention is to provide a method for producing nanoparticles encapsulating the wrinkle-improving effective substance.

It is still another object of the present invention to provide a cosmetic composition for improving wrinkles containing the nano-particles encapsulating the wrinkle-improving effective substance as an active ingredient.

In order to solve the above object,

The present invention relates to a wrinkle-improving effective ingredient (1 part by weight)

1 to 30 parts by weight of a glycol solubilizer;

1 to 30 parts by weight of a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)]; And

And 0.5 to 10 parts by weight of a skin penetration promoting agent.

The present invention also relates to a method for producing a wrinkle-improving active material, comprising the steps of adding a glycol solubilizing agent and a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethyleneoxide (PEO) ; And

And a step of adding and dispersing a skin penetration enhancer (step 2). The present invention also provides a method for producing nanoparticles encapsulating the wrinkle-improving effective substance.

Further, the present invention relates to a wrinkle-improving active material comprising 1 part by weight;

1 to 30 parts by weight of a glycol solubilizer;

1 to 30 parts by weight of a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)]; And

And 0.5 to 10 parts by weight of a skin penetration enhancer as an active ingredient. The present invention also provides a cosmetic composition for improving wrinkles containing nanoparticles encapsulating an effective wrinkle-reducing substance as an active ingredient.

The nanoparticles encapsulating the wrinkle-reducing effective substance according to the present invention include a skin penetration enhancer to solve the problem of low solubility in water of the wrinkle-improving active material, so that it is excellent in long-term stability and can further contain an antioxidant, It is possible to use the composition as a cosmetic composition for wrinkle improvement which is excellent in stability because the oxidation of the improvement effective substance is inhibited.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is an image showing the structure of nanoparticles encapsulating the wrinkle-improving effective substance provided by the present invention. Fig.
Fig. 2 is a photograph of Example 1 of the present invention prepared and diluted in water. Fig.
Fig. 3 is a photograph of Example 2 of the present invention prepared and diluted with water. Fig.
4 is a photograph of Example 3 of the present invention prepared and diluted with water.
Fig. 5 is a photograph of Example 4 of the present invention prepared and diluted in water. Fig.
Fig. 6 is a photograph of the sediment according to the lapse of time in Example 1 of the present invention. Fig.
7 is a photograph of the sediment according to the elapsed time of Example 2 of the present invention.
8 is a photograph of discoloration according to the third embodiment of the present invention over time.
FIG. 9 is a graph showing the results of measuring the particle size after diluting the Example 1 of the present invention with water. FIG.
10 is a graph showing the results of measuring the particle size after diluting the second embodiment of the present invention with water.
11 is a graph showing the result of measuring the particle size after diluting the third embodiment of the present invention with water.
Fig. 12 is a graph showing the results of measuring the particle size after diluting Example 4 of the present invention with water. Fig.
13 is a graph showing the results of observing changes in particle size with time and external temperature conditions in order to evaluate the long-term stability of Example 3 and Example 4. Fig.
14 is a graph showing the results of observing changes in the content with time and external temperature conditions in order to evaluate the long-term stability of Example 3 and Example 4 of the present invention.
FIG. 15 is a photograph showing a visual observation of a change occurring when the third embodiment of the present invention is left for 5 months under the respective external conditions. FIG.
FIG. 16 is a photograph showing a visual observation of a change occurring when the fourth embodiment of the present invention is left for 5 months under the respective external conditions. FIG.

Hereinafter, the present invention will be described in detail.

The present invention relates to a wrinkle-improving effective ingredient (1 part by weight)

1 to 30 parts by weight of a glycol solubilizer;

1 to 30 parts by weight of a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)]; And

0.5 to 10 parts by weight of a skin penetration enhancer, wherein the particle size of the nanoparticles has a particle size of 10-100 nm, more preferably 20-30 nm Particle size. The structure of nanoparticles encapsulating the wrinkle-improving active material provided by the present invention is shown in Fig.

The wrinkle-improving effective substance may be selected from the group consisting of silkworm extract, retinoids, vitamin A, vitamin C, betulinic acid, transforming growth factor (TGF), coenzyme Q10, ascorbic acid, Ononin, animal placenta-derived protein, centigrass extract, allium extract, chlorella extract and the like can be used.

At this time, the cocoon extract means powders, extracts, hydrolysates and the like of a larva, a pupa, and a cocoon-derived material of a silkworm.

More specifically, the powder of the silkworm-derived material can be obtained by pulverizing, i.e., pulverizing, silkworm cocoons, but is not limited thereto.

Also, the extract of the cocoon-derived material may be an oil component extracted using an extraction solvent selected from water, an organic solvent or a mixture thereof, and the organic solvent may be selected from methanol, ethanol, propanol, butanol, isopropanol, Alcohols having 1 to 5 carbon atoms such as 3-butylene glycol; Polar solvents such as ethyl acetate, acetone and the like; Non-polar solvents such as ether, chloroform, benzene, hexane and dichloromethane; Or a mixed solvent thereof may be used. Here, the extract of the cocoon-derived material includes fractions obtained by further fractionating the primary extract extracted using the extraction solvent with an extraction solvent having a different polarity. For example, the extract of the cocoon-derived material may be a fraction obtained by extracting through hot water extraction using water and then fractionating again with a polar solvent such as ethyl acetate or acetone, but the present invention is not limited thereto.

Further, the hydrolyzate of the silkworm-derived cocoa material is an acid hydrolyzate obtained by treating the silkworm with at least one selected from the group consisting of hydrochloric acid, phosphoric acid and sulfuric acid. The acid hydrolyzate is selected from the group consisting of silkworm pepsin, trypsin , Nutraceuticals, Alkalase, and the like, but the present invention is not limited thereto.

In addition, the glycol-based solubilizer dissolves the wrinkle-improving active material for micellization, and ethylene glycol, propylene glycol, tetraethylene glycol, polyethylene glycol, polypropylene glycol, polybutylene glycol and the like can be used. The glycol solubilizer is preferably contained in an amount of 1 to 30 parts by weight, more preferably 1 to 10 parts by weight, more preferably 2 to 8 parts by weight, based on 1 part by weight of the wrinkle- , And most preferably 4 to 6 parts by weight. If the glycerol solubilizer is contained in an amount of less than 1 part by weight based on 1 part by weight of the wrinkle improving active material, dispersion of the wrinkle improving active material is incomplete and there is a problem that release of the effective wrinkle improving material becomes difficult at the time of use, If the amount is more than 30 parts by weight based on 1 part by weight of the improvement active material, there is a problem that the improvement of the stability of the wrinkle improving active material is insufficient and the reagent is wasted.

Further, in order to further improve the solubility of the wrinkle-improving active material by the above-mentioned glycol-based solubilizing agent, glycofurol, polysorbate, cremophor, Solutol HS 15 and the like may be further mixed and used.

In addition, the triblock copolymer residues in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)] serve as micelle formation and are used in the form of poloxamer 184, poloxamer 185, poloxamer 188, Somer 124, Poloxamer 237, Poloxamer 338, Poloxamer 407, and the like. The triblock copolymer is preferably contained in an amount of 1 to 30 parts by weight, more preferably in an amount of 1 to 10 parts by weight, more preferably in an amount of 2 to 8 parts by weight, based on 1 part by weight of the wrinkle- And most preferably 4 to 6 parts by weight. If the triblock copolymer is contained in an amount of less than 1 part by weight based on 1 part by weight of the wrinkle improving active material, precipitation of the effective wrinkle improving substance occurs. In addition, There is a problem that the particle size increases and the stability is lowered.

Further, the skin penetration enhancer may be selected from the group consisting of diethylene glycol monoethyl ether, isopropyl myristate, isostearyl glyceryl ether, polysorbate 80, But are not limited to, sorbitan sesquioleate, caprylocaproyl macrogolglycerides, oleoyl macrogolglycerides, sodium lauryl sulfate, hexadecyltrimethylammonium bromide Hexadecyltrimethylammonium bromide, Cetrimide, Benzethonium chloride, Cetylpyridinium chloride, polyoxyl-10-stearyl ether (Brij 56), polyoxyl-20-cetyl ether (Brij 58), polyoxyl-2-stearyl ether (Brij 72), polyoxyl-10-stearyl ether (Brij 76), polyoxyl- (Brij 93), polyoxyl-10-oleyl ether (Brij 97), polyoxyl-35-castor oil (Cremophor EL), polyoxyl-40-hydrogeneate (Cremophor RH40), polyoxyl- And Cremophor RH60 may be used. Preferably, the skin penetration enhancer is contained in an amount of 0.5 to 10 parts by weight based on 1 part by weight of the wrinkle-improving effective substance. If the skin penetration enhancer is contained in an amount of less than 1 part by weight based on 1 part by weight of the wrinkle improving active material, there is a problem that an excellent effect of skin permeation can not be secured. There is a problem that the reagent is wasted because it contains an excessive amount of the skin penetration promoter required for permeation of the skin.

The nanoparticles encapsulated with the wrinkle improving active material may further contain 0.1 to 1 part by weight of an antioxidant to effectively prevent oxidation of the wrinkle improving active material over time. Examples of the nanoparticles include α-lipoic acid, but are not limited to, α-Tocopherol, Retinol, Glutathione, Butylated Hydroxy Toluene, Genistein, Quercetin, Propyl Gallate, Epigallocatechin gallate, Gallocatechin gallate, Silybin, Diosmetin, Kaempferol, Epicatechin, Galangin and the like can be used. have. If the antioxidant is contained in an amount of less than 0.1 part by weight based on 1 part by weight of the wrinkle improving active material, there is a problem that oxidation of the wrinkle improving active material can not be prevented. In the case where more than 1 part by weight There is a problem that the amount of the reagent is wasted because the excessive amount of the antioxidant is required to prevent oxidation of the wrinkle improving active material.

The present invention also relates to a method for producing a wrinkle-improving active material, comprising the steps of adding a glycol solubilizing agent and a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethyleneoxide (PEO) ; And

And a step of adding and dispersing a skin penetration enhancer (step 2). The present invention also provides a method for producing nanoparticles encapsulating the wrinkle-improving effective substance.

Hereinafter, a method for producing nanoparticles encapsulating an effective wrinkle-reducing substance according to the present invention will be described in detail.

In the method for producing nanoparticles encapsulating a wrinkle-improving effective substance according to the present invention, the step 1 is a step of adding a glycol-solubilizing agent and a [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO )] Type triblock copolymers are added and stirred.

At this time, the stirring temperature may be 20 to 100 ° C, preferably 40 to 80 ° C. The stirring may be performed by ultrasonic treatment.

In the method for producing nanoparticles encapsulating an effective substance for improving wrinkles according to the present invention, step 2 is a step of adding and dispersing a skin penetration promoting agent.

In this case, the step 1 may be performed before the step 2, and then the antioxidant may be added.

Further, the present invention relates to a wrinkle-improving active material comprising 1 part by weight;

1 to 30 parts by weight of a glycol solubilizer;

1 to 30 parts by weight of a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)]; And

And 0.5 to 10 parts by weight of a skin penetration enhancer as an active ingredient. The present invention also provides a cosmetic composition for improving wrinkles containing nanoparticles encapsulating an effective wrinkle-reducing substance as an active ingredient.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experiments are merely illustrative of the present invention, and the contents of the present invention are not limited by Examples and Experimental Examples.

< Example  1> Preparation of Nanoparticles Containing Wrinkle-Enhancing Active Substance and Glycol Solubilizer

30 mg of polyethylene glycol (PEG400) was added to 10 mg of retinol, dissolved and dissolved in water.

< Example  2> Preparation of Nanoparticles Containing Wrinkle-Enhancing Active Substance, Glycol Solubilizer and Triblock Copolymer

To 10 mg of retinol, 30 mg of polyethylene glycol (PEG400) was added and mixed. Then, 60 mg of triblock copolymer F127 (Poloxamer 407) was added, melted at a temperature of 60 ° C and dissolved in water.

< Example  3> Wrinkle improving effective substance, Glycol solubilizer , Triblock copolymer residues and skin permeation enhancers

After adding 30 mg of polyethylene glycol (PEG400) to 10 mg of retinol and mixing, 60 mg of triblock copolymer F127 (Poloxamer 407) and 30 mg of polyoxyl-35-castor oil (Cremophor EL) Lt; / RTI &gt; and melted in water.

< Example  4> Wrinkle improving effective substance, Glycol solubilizer , Triblock copolymer residues, skin Preparation of nanoparticles containing permeation enhancer and antioxidant

30 mg of polyethylene glycol (PEG400) was added to 10 mg of retinol and 2 mg of antioxidant alpha -tocopherol and mixed. Then, 60 mg of a triblock copolymer F127 (Poloxamer 407) and 60 mg of a polyoxyl-35-castor oil ) Was added, and melted at a temperature of 60 캜 and dissolved in water.

< Experimental Example  1> Particle size evaluation

ELS (ELS-Z, Otsuka, Japan) apparatus was used to evaluate the particle size of the nanoparticles prepared in Example 1-4 according to the present invention, and the results are shown in Tables 1 and 9-12 .

Example One 2 3 4 Particle Size (nm) 375.6 234.5 24.7 27.1

As shown in Table 1,

Examples 3 and 4, including skin permeation enhancers, can ensure particle sizes as small as 24.7 nm and 27.1 nm, respectively, while Examples 1 and 2, which do not include a skin permeation enhancer, have a particle size of 375.6 nm and 234.5 nm, respectively.

In addition, the nanoparticles prepared in Example 1-4 were visually observed, and the results are shown in Figs. 2-8.

As shown in Figures 2-8, Examples 1 and 2 were identified as very opaque solutions and precipitation occurred over time. On the other hand, Examples 3 and 4 including the skin permeation enhancer were found to be in a transparent solution state, but in Example 3, which did not contain an antioxidant, the color changed with time.

< Experimental Example  2> Particle size measurement for long-term stability evaluation

For the measurement of the particle size for evaluating the long-term stability of the nanoparticles prepared in Examples 3 and 4 according to the present invention, the nanoparticles prepared in Examples 3 and 4 were exposed to sunlight, 4 ° C, room temperature (25 ° C) And the particle size was measured with time at 50 캜. The results are shown in Table 2, FIG. 13, and FIG. 15-16.

Unit (nm) sunshine 4 ℃ Room temperature (25 캜) 50 ℃ Example 3 0 months 24.7 24.7 24.7 24.7 5 months 15.4 21.3 20.5 14.3 Example 4 0 months 27.1 27.1 27.1 27.1 5 months 27.5 27.3 27.0 27.3

As shown in Table 2,

In Example 3 which did not contain an antioxidant, the particle size was changed due to external conditions and showed discoloration, indicating that the long-term stability was not excellent. However, in Example 4 including an antioxidant, It was found that there was almost no change in size, indicating that the long-term stability was excellent.

Therefore, it can be seen that the nanoparticles encapsulating the wrinkle-improving effective substance according to the present invention preferably include an antioxidant.

< Experimental Example  3> Measurement of retinol content for long term stability evaluation

For the measurement of the particle size for evaluating the long-term stability of the nanoparticles prepared in Examples 3 and 4 according to the present invention, the nanoparticles prepared in Examples 3 and 4 were exposed to sunlight, 4 ° C, room temperature (25 ° C) And the change of retinol content by HPLC (High-performance liquid chromatography) was measured at 50 ° C. The results are shown in Table 3 and Fig.

unit(%) sunshine 4 ℃ Room temperature (25 캜) 50 ℃ Example 3 0 months 100.1 100.1 100.1 100.1 5 months 32.1 48.3 51.2 25.1 Example 4 0 months 100.5 100.5 100.5 100.5 5 months 98.2 100.1 99.1 95.8

As shown in Table 3,

In Example 3, which did not contain an antioxidant, the retinol content was changed by external conditions, whereas in Example 4 containing an antioxidant, the change in retinol content due to external conditions hardly occurred, It is very good.

Therefore, it can be seen that the nanoparticles encapsulating the wrinkle-improving effective substance according to the present invention preferably include an antioxidant.

Claims (10)

1 part by weight of wrinkle improving active substance;
1 to 30 parts by weight of a glycol solubilizer;
1 to 30 parts by weight of a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)]; And
0.5 to 10 parts by weight of a skin penetration enhancer.
The method according to claim 1,
The wrinkle-improving effective substance may be selected from the group consisting of cocoon extract, retinoids, vitamin A, vitamin C, betulinic acid, transforming growth factor (TGF), coenzyme Q10, ascorbic acid, Wherein the nanoparticle is at least one selected from the group consisting of Ononin, an animal placenta-derived protein, centigrass extract, allium extract, and chlorella extract.
The method according to claim 1,
Wherein the glycol solubilizer is at least one selected from the group consisting of ethylene glycol, propylene glycol, tetraethylene glycol, polyethylene glycol, polypropylene glycol and polybutylene glycol.
The method according to claim 1,
The triblock copolymer residues in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -Polyethylene Oxide (PEO)] include Poloxamer 184, Poloxamer 185, Poloxamer 188, Poloxamer 124, Poloxamer 237, 338 and poloxamer 407. The nanoparticle according to claim 1,
The method according to claim 1,
The skin penetration enhancer may be selected from the group consisting of diethylene glycol monoethyl ether, isopropyl myristate, isostearyl glyceryl ether, polysorbate 80, sorbitan But are not limited to, sorbitan sesquioleate, caprylocaproyl macrogolglycerides, oleoyl macrogolglycerides, sodium lauryl sulfate, hexadecyltrimethylammonium bromide Cetrimide, benzethonium chloride, cetylpyridinium chloride, polyoxyl-10-stearyl ether (Brij 56), polyoxyl-20-cetyl ether (Brij 58) , Polyoxyl-2-stearyl ether (Brij 72), polyoxyl-10-stearyl ether (Brij 76), polyoxyl- (Brij 93), polyoxyl-10-oleyl ether (Brij 97), polyoxyl-35-castor oil (Cremophor EL), polyoxyl-40-hydrogeneate (Cremophor RH40) and polyoxyl- (Cremophor RH60). &Lt; / RTI &gt;
The method according to claim 1,
Wherein the nanoparticles encapsulating the wrinkle improving active material may further contain 0.1 to 1 part by weight of an antioxidant based on 1 part by weight of the wrinkle improving active material.
The method according to claim 6,
The antioxidant may be selected from the group consisting of? -Lipoic acid,? -Tocopherol, retinol, glutathione, butylated hydroxy toluene, genistein, quercetin, Quercetin, Propyl gallate, Epigallocatechin gallate, Gallocatechin gallate, Silybin, Diosmetin, Kaempferol, Wherein the nanoparticle is at least one selected from the group consisting of Epicatechin and Galangin.
(Step 1) of adding a glycoleol solubilizing agent and a triblock copolymerization agent in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)] to the wrinkle improving active material; And
A method for preparing nanoparticles encapsulating an effective wrinkle-reducing substance according to claim 1, comprising the step of adding and dispersing a skin penetration promoting agent (step 2).
9. The method of claim 8,
Wherein the method further comprises the step of adding an antioxidant prior to carrying out the step 2.
1 part by weight of wrinkle improving active substance;
1 to 30 parts by weight of a glycol solubilizer;
1 to 30 parts by weight of a triblock copolymer in the form of [polyethylene oxide (PEO) -polypropylene oxide (PPO) -polyethylene oxide (PEO)]; And
And 0.5 to 10 parts by weight of a skin penetration promoting agent as an active ingredient.

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