KR101002433B1 - nano-emulsion comprsing vitamine A or its derivatives and acetyl hexapeptide-3, and anti-wrinkle cosmetic preparation including the same - Google Patents

Abstract

The present invention relates to a nano-emulsion and a cosmetic composition for improving skin wrinkles containing the same, containing vitamin A and its derivatives and acetyl hexapeptide-3, wherein the nano-emulsion cosmetic composition according to the present invention comprises vitamin A and its derivatives and acetyl hexa In addition to peptide-3, oil (A), surfactant (B), and higher alcohol (C) are related to a nanoemulsion comprising a weight ratio of (A) / [(B) + (C)] at 0.5 to 9. .

The nanoemulsion cosmetic composition according to the present invention not only has excellent skin aging, anti-wrinkle effect and transdermal absorption effect, but also provides excellent anti-wrinkle effect for long-term storage stability, skin safety, and ease of use.

Vitamin A, acetyl hexapeptide-3, nanoemulsion, skin wrinkle improvement, anti wrinkle,

Description

Nano-emulsion comprsing vitamine A or its derivatives and acetyl hexapeptide-3, and anti-wrinkle cosmetic preparation including the same}

The present invention relates to a nano-emulsion and cosmetic composition for improving wrinkles containing vitamin-A and its derivatives and acetyl hexapeptide-3, and more particularly, to a micro-containing containing vitamin-A and its derivatives and acetyl hexapeptide-3. The present invention relates to a wrinkle-improving nanoemulsion composition which improves permeability absorption as well as long term storage stability, skin safety and ease of use by preparing an emulsion.

In general, theories explaining skin aging include the theory of mutagenesis of somatic cells, the error occurrence theory of gene replication, the gene program theory, the free radical theory, the autoimmune response theory, the accumulation of toxic substances, and the modification theory of dermal constituent proteins. , C & T, 110, 94, 1994). The above theories are all involved in skin aging, and free radicals are known to be the main cause of skin aging. As a result of skin aging, wrinkles, skin elasticity, senile plaques, etc. are finally formed in the skin appearance, of which wrinkles are the most representative. Free radicals are chemically highly reactive chemicals that occur when the skin is irradiated with UV light or during the respiration of cells in the skin.

As described above, most of the free radicals related to skin aging are reactive oxygen species (ROS), for example, superoxide radical, hydrogen peroxide (H ₂ O ₂ ), hydroxy radical (hydroxy radical). ), Singlet oxygen, and the like. These reactive oxygen species are generated during ultraviolet irradiation or respiration process to cause peroxidation of lipids, which are components of cell membranes, through a multi-step chain reaction.

Lipid peroxidation generates various active species such as radicals, peroxides, aldehydes, epoxides, and the like, and damages DNA, RNA, proteins, cell membranes, and cell structures. Toxicity of these reactive oxygen species is considered to be a major cause of cancer, tissue damage, and aging (Black HS, Photochem photobiol, 46 (2), 213, 1987). Peroxidation of the membrane lipids that make up the cell changes the structure and fluidity of the cell membrane, increases the permeability of cellular material, leaks of lysosome enzymes, inactivation of membrane enzymes and transport proteins, and covalent crosslinking of lipids and proteins. ), Protein chain cleavage, DNA damage, and mutations. Among these reactions, peroxidation of dermal cell membranes and modification of dermal constituent proteins are known to be most closely associated with skin aging. However, in spite of many of these studies, it is not yet known how each factor causes skin wrinkles, and thus, research on the mechanism of wrinkle formation is still ongoing.

Materials used for inhibiting skin aging to date include enzymes such as superoxide dismutase or catalase; fat-soluble or water-soluble antioxidants such as α-tocopherol (vitamin E), β-carotene, ascorbic acid (vitamin C), or glutathione, or radical scavengers. And, they mainly have a preventive effect of suppressing wrinkle formation due to skin aging. On the other hand, vitamin A, which has recently been spotlighted as an anti-aging substance, has a healing effect to alleviate wrinkles generated through the enhancement of collagen synthesis in the dermis, and the wrinkle relief effect appears faster than that of antioxidants, and additional effects such as skin cell regeneration and whitening. Efficacy has been reported (R. Hermitte, C & T, 107, 63, 1992).

Wrinkles are formed as a result of the movement of muscles in a particular direction, which is a result of scaling for a very long time. In addition, wrinkle formation is caused by various factors such as age, external environment, UV irradiation, and the like, and the change between the dermis and the epidermis due to skin aging is related to wrinkle formation.

Until now, the most effective substance for wrinkle removal is vitamin A (retinoid), and recently, peptides are used to improve wrinkles by controlling the triggering pathways such as changes in collagen triple helix structure and elastin destruction using wrinkles. Research on improved cosmetics is actively progressing. The vitamin A is known to penetrate the skin to promote cell production and increase the synthesis of collagen fibers to remove and reduce wrinkles (Muneo Tanaka. Recent development of skincare products in Japan from the Bio-Science standpoint Fragrance Journal. , 9, 1994 pp 13-23., 光 井 武夫.

The vitamin A is widely known as a substance having the effect of removing wrinkles through cell regeneration and collagen synthesis (Raoul Hermitte. Aged Skin, Retinoids, and Alpha Hydroxy Acids Cosmetics & Toiletries ., Vol. 107, Jul. 1992 pp 63-67), and also reports that light aging can be improved (Webb et al. Topical Tretinoin Improves Photoaged Skin, JAMA, 259, vol. 4, Jan, 22/29, 1988, pp95, 527-532). ).

Recently, a procedure for removing wrinkles by injecting a substance involved in muscle paralysis called botulinum toxin (Botox) into the wrinkle area has been popularized, and thus, studies on botulinum toxin are being actively conducted (Montecucco, C. and Schiavo). , G. (1994) .Mechanism of action of tetanus and botulinum neurotoxins.Mol. Microbiol. 13, 1-8 Jankovic, J. and Brin, MF (1997) .Botulinum toxin: historical perspective and potential new indications.Muscle Nerve Suppl 6, pp129-145).

In addition, the peptide component is a substance applying the wrinkle improvement reaction pathway of botulinum toxin to cosmetics and improves wrinkles through muscle paralysis, thus acting through a wrinkle improvement mechanism different from vitamin A. That is, catecholamines (peptide derivative neurotransmitters involved in cellular signaling systems) that interfere with the formation of SNARE (recognition receptors present on the surface of vesicles involved in the transport of substances in cells) complexes involved in neurotransmission pathways. By suppressing the release of the muscles to suppress the contraction of the skin when applied to improve wrinkles. European Patent Publication No. 1,180,524 A1 discloses an amino acid sequence excellent in the wrinkle improvement effect of the peptide. In particular, 6 to 19 peptides among the amino acid sequences describe the most excellent wrinkle improvement effect.

Therefore, there is a need for further studies on compositions having excellent skin removal effects and their wrinkle removal mechanisms.

In general, cosmetic formulations are commonly used for solubilization, emulsification, gels, lotions, lotions, creams, essences, etc. Recently, research on nanoemulsion formulations for increasing transdermal absorption has been actively conducted.

  The production of microemulsions containing vitamin-A, derivatives thereof, and acetyl hexapeptide-3, in which the emulsified particles are characterized by a fine nanoemulsion, has a very poor preservation stability and a large amount of surfactant in the conventional general emulsification method. Because of the stimulus problem, it was almost impossible to use.

In order to overcome this problem, Peterson, Hamill, Macmahon, etc. introduced a new emulsification method that emulsifies glycerin and olive oil by using a nonionic surfactant and various surfactants, but did not make a stable emulsion.

Others include Japanese Patent Laid-Open No. 57-29213 and Japanese Patent Laid-Open No. 7-20543. They first dissolved the hydrophilic nonionic surfactant in an aqueous solvent and then added oil to prepare an emulsion first, followed by the addition of water in the next step to form the final emulsion. They dissolve the surfactants in polyhydric alcohols, which are water-soluble solvents rather than oils, which significantly lower the interfacial tension between oil and water in the final emulsion preparation, allowing the surfactants to be oriented efficiently at the interface, thus making formulations with relatively small amounts of surfactants. Although completed, only short-term preservation stability has been secured, thus practical commercialization has not been achieved. In the case of preparing the nanoemulsion according to a conventionally known non-aqueous method, there is a problem in that the stability with time is low and there is no commerciality.

The technical problem to be achieved by the present invention is an average emulsified particle size by efficiently orienting a derivative including vitamin-A and its derivatives, acetyl hexapeptide-3, a higher alcohol, a surfactant, and an oil at an interface even by a general emulsification method. To provide a nanoemulsion having 100-1000 nm.

It is also an object of the present invention to provide a wrinkle improving nanoemulsion cosmetic composition comprising the nanoemulsion composition, excellent transdermal absorption fastness, long-term storage stability, skin safety, ease of use.

In order to achieve the above technical problem, the present invention includes vitamin A and derivatives thereof, acetyl hexapeptide-3, oil (A), surfactant (B), and alcohol (C) having 12 to 22 carbon atoms, The weight ratio of (A) / [(B) + (C)] is 0.5 to 9, and the nanoemulsion having excellent stability over time is 100 to 100 nm in average particle size. The emulsion according to the invention is an O / W emulsion.

The present invention provides a cosmetic composition for improving wrinkles comprising 1-100 parts by weight of the nanoemulsion. The cosmetic composition may further include a moisturizing solution, a lotion, or a macro emulsion having an average particle size of more than 1000 nm.

The present invention provides a nanoemulsion using a general emulsification method in which a derivative including vitamin-A and its derivatives, acetyl hexapeptide-3, a higher alcohol, a surfactant, and an oil is efficiently oriented at the interface to have a smaller particle size. In addition, by adjusting the content of the composition at an appropriate ratio, it provides a wrinkle-improving nano-emulsion cosmetic composition excellent in transdermal absorption fastness, long-term storage stability, skin safety, ease of use.

Hereinafter, the present invention will be described in detail.

Emulsions are classified into three types: microemulsion (5-100 nm), nanoemulsion (100-1000 nm), and macroemulsion (more than 1000 nm), depending on the average size of the emulsion particles. That is, in the present invention, the nanoemulsion means an emulsion of emulsified particles of 100 to 1000 nm. In order to improve transdermal absorption fastness, the size of emulsified particles should be made small. Vitamin-A, its derivatives, and acetyl hexapeptide-3 have high molecular weight structurally, so the compatibility with the constituents forming the formulation is very stable. It is important.

In the emulsion composition of the present invention, the weight ratio of (A) / [(B) + (C)] is 0.5 to 9, preferably 1 to 5. If the weight ratio is less than 0.5, the content ratio of the surfactant is greater than that of the oil, so that the skin irritation is not only increased, but the solubilization type of the solubilization type, which is not a nanoemulsion, is made. If the (A) / [(B) + (C)] weight ratio exceeds 9, the amount of surfactant is too small compared to the oil, and the excess oils that are not properly oriented at the interface float to the surface, resulting in poor formulation stability. . The excellent long-term storage stability of the nanoemulsion cosmetic composition according to the present invention, as described above, by controlling the weight ratio of (A) / [(B) + (C)], effectively oriented higher alcohols and surfactants to the interface. Rather, the emulsified particles do brown motion.

The vitamin A and its derivatives and acetyl hexapeptide-3 is preferably used in a ratio of 10,000 to 1, vitamin A and its derivatives / acetyl hexapeptide-3 for skin safety and wrinkle improvement effect, more preferably 1,000 It is used in ratio of 10 to. If the vitamin A and its derivatives / acetyl hexapeptide-3 is less than 1, the content of vitamin A is too small, the synergistic effect is significantly reduced, if the content exceeds 10,000, the content of acetyl hexapeptide-3 is too small There is a problem that the synergistic effect of wrinkle improvement is reduced.

In the nano-emulsion cosmetic composition of the present invention, the vitamin A and its derivatives act to alleviate skin wrinkles by enhancing intracellular collagen synthesis, cell regeneration, and the like, and vitamin A and its derivatives known to perform such a function All are applicable to the present invention. Examples of the vitamin A and its derivatives include water-soluble vitamin A and derivatives thereof such as polyethoxylateddretinamide, or oil-soluble vitamin A such as retinoic acid, retinol, retinyl acetate, or retinyl palmitate, and derivatives thereof. Can be used. The said vitamin A and its derivative (s) contain 1 type (s) or 2 or more types.

The vitamin A and its derivatives are preferably included in 0.0001 to 10.0 parts by weight based on 100 parts by weight of the composition. If the content is less than 0.0001 parts by weight, wrinkle reduction through collagen synthesis enhancement and cell regeneration is insignificant. If it is more than 10.0 parts by weight, skin side effects are severe and synergistic effects with acetyl hexapeptide-3 are insignificant. have.

The acetyl hexapeptide-3 used in the present invention functions to improve wrinkles through muscle paralysis. The amino acid sequence of acetyl hexapeptide-3 is acetyl glutamyl-glutamyl-methionyl-glutamyl-arginyl-arginylamine (acetly glutamyl-glutamyl-methionly-glutamyl-arginyl-arginylamine). The acetyl hexapeptide-3 is preferably included in an amount of 0.00001 to 0.001 parts by weight based on 100 parts by weight of the cosmetic composition. If the content is less than 0.00001 parts by weight, the effect of muscle paralysis is insignificant, if it exceeds 0.001 parts by weight there is a problem that the wrinkle improvement effect is no longer increased and the synergistic effect with vitamin A is insignificant.

In a preferred embodiment of the present invention, the emulsion composition of the present invention is an alcohol (C) having 1 to 50 parts by weight of oil (A), 0.5 to 20 parts by weight of surfactant (B) and 12 to 22 carbon atoms based on 100 parts by weight of the composition. 0.1 to 5 parts by weight, vitamin A and derivatives thereof from 0.0001 to 10 parts by weight and acetyl hexapeptide-3 from 0.00001 to 0.001 parts by weight.

In the nanoemulsion composition according to the present invention, one or more selected from vegetable oil, mineral oil, silicone oil and synthetic oil may be used as the oil. The content of oil in the composition according to the present invention may be 1-50 parts by weight with respect to 100 parts by weight of the composition, preferably 3 to 45 parts by weight. If it is less than 1 part by weight, the amount of oil is so small that the usability as an emulsion (appliability, softness and tightness, etc.) is poor, and if it exceeds 50 parts by weight, the amount of oil is too large, making nanoemulsion difficult and poor stability over time.

In the nanoemulsion composition according to the present invention, the higher alcohol may be used by mixing one or more alcohols having 12 to 22 carbon atoms, for example, lauryl alcohol, cetyl alcohol, cetearyl alcohol, stearyl alcohol, But not limited to henyl alcohol.

In addition, conventional surfactants may be used as the surfactant, but in particular, sorbitan monostearate, cetheares-20, sorbitan sesquistearate, sorbitan sesquioleate, lipophilic glyceryl stearate, octyldodeces Nonionic interfaces, such as -16, polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 65, polysorbate 80, polysorbate 85, polyoxyethylene-cured castor oil, sebum fiji copolymer Preference is given to using activators.

The nanoemulsion cosmetic composition according to the present invention may further contain ceramide, ceramide derivatives such as ceramides 1 to 6, cholesterol derivatives such as cholesterol, cholesterol esters, cholesterol ethers, and the like. These materials are added to higher alcohols and serve as secondary surfactants to strengthen the interfacial membrane, thereby improving long-term shelf life. The preferred content of ceramide or ceramide derivative is 0.001 to 2 parts by weight, and the preferred content of cholesterol or cholesterol derivative is 0.01 to 2 parts by weight.                     

Nanoemulsion according to the present invention may contain a wax in the oil phase (oil phase) for viscosity control, and may contain a hydrophilic polymer in the water phase. The preferred content of the wax in the nanoemulsion is 0.01 to 5 parts by weight, and the preferred content of the hydrophilic polymer may contain 0.01 to 2 parts by weight. As waxes usable in the present invention, one or more selected from animal waxes, vegetable waxes and mineral waxes can be used. Examples of hydrophilic polymers include: carbomer, xanthan gum, gelatin, carrageenan, starch, gum arabic, carboxymethylcellulose. , But not limited to, ethyl cellulose, bentonite, sodium polyacrylate, and the like.

The nanoemulsion cosmetic composition according to the present invention can be used by itself, but can be used by mixing the nanoemulsion based on other cosmetic compositions, macro emulsion, and also can be used in the lotion, moisturizing liquid phase depending on the application. Therefore, examples of the formulation of the cosmetic composition including the nano-emulsion of the present invention include, but are not limited to, soft cosmetics, nourishing cosmetics, nourishing cream, massage cream, essence, pack, emulsion, oil gel, and the like. Preferably, the cosmetic composition of the present invention may be used by adding the nanoemulsion to the general macroemulsion, and even if the nanoemulsion is added to the softening longevity, essence and cream of the macroemulsion, it has excellent transdermal absorption fastness and long-term storage stability. The object of the invention can be achieved. This is because nanoemulsions with small particles are stably dispersed in the general emulsion.

In addition, the cosmetic composition may be formulated as necessary to the usual ingredients, such as oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc., which are formulated in general skin cosmetics. .

The content of the nanoemulsion in 100 parts by weight of the cosmetic composition according to the present invention is 1-100 parts by weight. When added to the general emulsion based on the nanoemulsion, the more preferable content of the nanoemulsion is 1 to 50 parts by weight based on the total weight of the composition. Less than 1 part by weight of vitamin-A, its derivatives, and acetyl hexapeptide-3 content is difficult to expect a clear whitening effect, and adding more than 50 parts by weight of the nanoemulsion to the macroemulsion to control the viscosity by nanoemulsion This is because it becomes more inconvenient than preparing the whole emulsion.

Hereinafter, the present invention will be described in detail with reference to Examples and Comparative Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below.

Examples 1 to 3

According to the composition shown in Table 1, each component was divided into an oil phase and an aqueous phase, and heated to 70 to 90 ° C., respectively. Then, an oil phase was added to the aqueous phase and stirred with a homomixer. O / W emulsions containing peptides were prepared.                     

Raw material name (weight part) Example 1 Example 2 Example 3 Liquid paraffin 7.0 5.0 5.0 Trioctanoine 2.0 5.0 20.0 Phenyltrimethicone 1.0 - 5.0 Ceteares-20 2.5 2.0 2.0 Octyldodec-16 - 0.5 1.0 Behenyl Alcohol 0.5 0.5 1.5 Cetearyl Alcohol 0.5 0.5 1.5 cholesterol - 1.0 1.0 Beeswax - 0.2 0.5 Ceramide-1 0.1 0.1 0.2 Ceramide-2 - 0.2 0.2 Vitamin-A Palmitate - 0.2 0.2 1,3-butene glycol 2.0 5.0 3.0 Dipropylene glycol - 2.0 2.0 Concentrated glycerin 1.5 1.0 1.0 ethanol - 3.0 5.0 Polyethoxylatedretinamide 0.1 1.0 0.1 Acetylhexapeptide 0.001 0.05 0.005 antiseptic q.s q.s q.s incense q.s q.s q.s Triethanolamine q.s q.s q.s Pigment q.s q.s q.s Carboxy vinyl polymer (1% aqueous solution) - - 10.0 Purified water to 100 to 100 to 100

The difference between Examples 1 and 2 is that two types of surfactants are used, and the viscosity of the nanoemulsion is increased by wax, which is a wax component, and Example 3 is an increase in the oil content and the degree due to the water-soluble polymer. Reference). In the above examples, the weight ratio of (A) / [(B) + (C)] is 2.86 for the emulsion of Example 1, 2.08 for the emulsion of Example 2, and 4.05 for the emulsion of Example 3.

Examples 4-6

According to the composition shown in Table 2 below, a general emulsion containing the nanoemulsion of Example 2 was prepared.                     

Raw material name (weight part) Example 4 Example 5 Example 6 Purified water to 100 to 100 to 100 Triethanolamine 0.15 0.15 0.15 Carboxy vinyl polymer (1% aqueous solution) 10.0 10.0 10.0 Dark glycerin 2.0 2.0 2.0 Propylene glycol 3.0 3.0 3.0 Paraoxybenzoic Acid Ester 0.2 0.2 0.2 Cetostearyl alcohol 0.8 0.8 0.8 Glyceryl Monostearate 1.5 1.5 1.5 Sorbitol Monostearate 0.3 0.3 0.3 POE (20) sorbitan monostearate 1.5 1.5 1.5 Paraoxybenzoic acid propyl 0.1 0.1 0.1 Isocetyl octinoate 5.0 5.0 5.0 Isocetil Myristate 7.0 7.0 7.0 Nanoemulsion of Example 2 1.0 10.0 50.0

Comparative Examples 1 to 3

Comparative Example 1 was prepared by the same general emulsification method as in the examples in which each component was divided into an oil phase and an aqueous phase and heated to 75 ° C. according to the composition shown in Table 3 below, and then the oil phase was added to the aqueous phase and stirred with a homomixer. In Comparative Example 1, an emulsion was prepared according to the general emulsification method without adding a higher alcohol component, unlike in Example 1, and thus a macro emulsion is formed because a nanoemulsion was not prepared by the general emulsification method.

In preparing the samples of Comparative Example 2 and Comparative Example 3, the first step is to add a hydrophilic nonionic surfactant to the water-soluble solvent, and then add an oil phase to prepare a type emulsion in the water-soluble solvent, and the second step to add purified water to the emulsion The non-aqueous emulsification method of Japanese Patent Laid-Open No. 57-29213 for preparing an oil-in-water emulsion was used. In Comparative Example 2 was prepared according to the conventional non-hydrating method of preparing a nano-emulsion, the nano-emulsion size of 900nm particle size is obtained, but the stability over time is very poor, there is no product value (see Experimental Example below). When the oil content in Comparative Example 3 increases, the nanoemulsion cannot be prepared by the nonaqueous emulsification method, and only a macroemulsion is formed.

Raw material name (weight part) Comparative Example 1 Comparative Example 2 Comparative Example 3 Liquid paraffin 7.0 5.0 5.0 Jojoba You 2.0 5.0 20.0 Phenyltrimethicone 1.0 - 5.0 Ceteares-20 2.5 2.0 2.0 Octyldodec-16 - 0.5 0.5 Vitamin-A Palmitate 0.1 0.2 0.2 1,3-butene glycol 2.0 5.0 5.0 Propylene glycol - 4.5 4.5 Concentrated glycerin 1.5 3.5 3.5 Carboxy Vinyl Polymer - - 0.1 ethanol 3.0 3.0 5.0 Polyethoxylatedretinamide 0.1 0.1 0.1 Acetylhexapeptide 0.001 0.0005 0.005 Triethanolamine q.s q.s q.s antiseptic q.s q.s q.s incense q.s q.s q.s Pigment q.s q.s q.s Purified water to 100 to 100 to 100

The prepared Examples 1 to 6 and Comparative Examples 1 to 3 were evaluated by dividing the average emulsion particle size, long-term preservation stability, skin safety, percutaneous absorption, wrinkle improvement effect, ease of use.

Experimental Example 1: Change in average emulsion particle size

The emulsion particle size of the nanoemulsion of Example 1 was measured using an emulsified particle size measuring device (PARTICLE SIZER MODEL 370 NICOMP) while changing the temperature and duration, and then the standard deviation () of the measured distribution value is shown in Table 4. Indicated.

Item 1 week (unit: nm) 1 month 6 months 0 ℃ 53.52 54.62 54.69 40 ℃ 53.52 55.57 56.60 45 ℃ 53.54 55.60 57.70 50 ℃ 53.55 56.59 58.70 Cycle 53.55 56.60 58.72 Room temperature 53.52 53.57 53.59

 As shown in Table 4, the emulsion of Example 2 maintained stability in which the average particle size hardly changed even after long-term storage at low temperature, room temperature and high temperature.

In addition, the emulsion of Comparative Examples 1 to 3 was measured in the same manner as in the above Example to obtain the standard deviation of the measured distribution value by measuring the emulsified particle size change, Comparative Example 1 is 2500nm, Comparative Example 2 is 800nm, Comparative Example 3 Was poor at 2320 nm.

The average particle sizes of Examples 1 to 3 were 200 nm, 180 nm and 410 nm, respectively. On the other hand, the average particle sizes of Comparative Examples 1 to 3 are 1500 nm, 900 nm and 2500 nm, respectively, and most of the macroemulsion was produced, and Comparative Example 1 had poor aging stability.

Experimental Example 2: Long-term Preservation Stability

The following evaluation of long-term preservation stability in Examples 1 to 6 and Comparative Examples 1 to 3 in units of 1 week, 1 month and 6 months at 0 ° C., 40 ° C., 45 ° C., 50 ° C., a thermostat and a room temperature of Cycle. Evaluation by reference is shown in Table 5 below.

<Evaluation Criteria>

O: good, △: unstable, ×: separation                     

As shown in Table 5, Examples 1 to 6 were superior to the long-term storage stability as compared to Comparative Examples 1 to 3.

Experimental Example 3: Skin Safety

Skin safety was evaluated through skin intelligence experiments for Example 3 and Comparative Example 3. A total of 90 patients (73 women and 17 men) who had experienced skin side effects by cosmetics were applied to the patient's back, and after 48 hours, the patch was removed and the first 1 to 2 hours later. Judgment was made, and after 72 hours or 96 hours, the results are shown in Table 6 below.                     

Item Positive reaction % Example 3 1 (+) 1.1 Comparative Example 3 5 (+) 5.7

As shown in Table 6, the sample of Example 3 according to the present invention has excellent skin safety, but it can be seen that the sample of Comparative Example 3 has severe skin side effects.

Experimental Example 4 Percutaneous Absorption Efficiency

Transdermal absorption efficiency was evaluated through transdermal absorption for polyethoxylatedretinamide. That is, in vitro skin permeation tests were performed using the skin of hairless mice. 48 microliters of the samples of Examples 1, 3, and 5 and Comparative Examples 1 to 3 were applied to the skin surface having a diameter of 15.0 mm, and the polyethoxylatedretin permeated after 24 hours using a Hanson Microette. The amount of amide was quantified by liquid chromatography and shown in Table 7.

sample Example 1 Example 3 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Transmittance (%) 15.55 14.66 15.64 8.55 9.65 7.55

As shown in Table 7, it can be seen that the percutaneous absorption efficiency according to the embodiment is superior to the comparative example. This is because the size of the emulsified particles according to the embodiment is smaller than that of the comparative example, so that it is more easily transmitted to the skin.

Experimental Example 5: Wrinkle improvement effect

Wrinkle improvement effect was measured using the cosmetics prepared in Examples 1, 3, 5 and Comparative Examples 1, 2, 3.                     

Eighty women aged 40 to 60 years were applied twice a day to the cosmetic compositions prepared in Examples 1, 3, 5 and Comparative Examples 1, 2, and 3 uniformly around the eyes. Wrinkles (crow's feet) were performed for a total of three months.

The cosmetic composition prepared in Examples 1, 3 and 5 was applied to the left eye area of the test subject, and the cosmetic composition prepared in Comparative Examples 1, 2 and 3 was applied to the right eye area. After 3 months, the wrinkles were visually judged by visual judgment (no improvement, slight improvement, moderate improvement, significant improvement compared with the control group), and eye wrinkle wrinkles before and after applying the cosmetic composition. The depth (μm) of the wrinkles was measured, and the results are shown in Tables 8 and 9 below.

division No improvement Minor improvements Improvement in severity A significant improvement Example 1 One 2 3 4 Example 3 0 2 4 4 Example 5 One 4 2 3 Comparative Example 1 3 3 3 One Comparative Example 2 3 4 3 0 Comparative Example 3 2 3 4 One

division Example 1 Example 3 Example 5 Comparative Example 3 Before applying cosmetic composition 325 ± 41 308 ± 39 86 ± 33 295 ± 35 90 days after application of cosmetic composition 179 ± 41 255 ± 32 247 ± 36 276 ± 37 Wrinkle depth reduction rate (%) 14.1% 17.2% 13.6% 6.4%

Through Table 8, the cosmetic compositions of Examples 1, 3 and 5 according to the present invention showed an excellent wrinkle relief effect compared to the cosmetic compositions of Comparative Examples 1, 2 and 3.

In addition, through Table 9, the cosmetic compositions of Examples 1, 3, 5 according to the present invention, while the depth of the wrinkles is reduced by 13.6 ~ 17.2%, the cosmetic composition of Comparative Example 3 was reduced by 6.4%. Through this, the cosmetic composition according to the present invention was confirmed that the excellent wrinkle depth reduction effect.

Experimental Example 6: Ease of Use

Ease of use was evaluated for Comparative Examples 1-3 and Examples 1-3. In other words, each of the comparative sample and the example sample (comparative example 1, example 1, (comparative example 3, example 3), etc.) by five persons was sensually evaluated for a total of 45 subjects. The results are shown in Table 10 below.

<Evaluation Criteria>

1: very bad, 2: bad, 3: slightly bad, 4: normal

5: slightly good, 6: good, 7: very good

Item Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Ease of application when applying skin 5.7 5.2 5.7 4.7 5.1 5.2 Skin adhesion 5.2 5.0 5.0 5.0 4.3 4.7 Absorbent 5.9 5.2 5.0 3.5 4.5 4.0 flexibility 5.7 5.6 5.1 4.7 4.7 4.1

As shown in Table 10, Examples 1 to 3 were excellent in absorbency and flexibility, it was evaluated that the ease of use.

As such, the present invention relates to a vitamin emulsion containing a vitamin-A, a derivative thereof, and an acetyl hexapeptide-3, and a cosmetic composition for improving wrinkles comprising the same. The present invention relates to a nano-emulsion and a cosmetic composition for improving skin wrinkles including the same, wherein the nano-emulsion cosmetic composition according to the present invention not only has excellent skin aging, anti-wrinkle effect and transdermal absorption effect, but also long-term storage stability, skin safety, and Ease of use provides excellent wrinkle improvement.

Claims (12)

At least one vitamin A or derivative of vitamin A selected from the group consisting of polyethoxylateddretinamide, retinoic acid, retinol, retinyl acetate, and retinyl palmitate; Acetyl hexapeptide-3; At least one oil (A) selected from the group consisting of vegetable oils, mineral oils, silicone oils, and synthetic oils; Sorbitan monosulfate, ceteareth-20, sorbitan sesquistearate, sorbitan sesquioleate, lipophilic glyceryl stearate, octyldodeces-16, polysorbate 20, polysorbate 40, polysorbate 60 At least one nonionic surfactant (B) selected from the group consisting of polysorbate 65, polysorbate 80, polysorbate 85, polyoxyethylene-cured castor oil, and sebum fiji copolymer; And at least one alcohol having 12 to 22 carbon atoms (C) selected from the group consisting of lauryl alcohol, cetyl alcohol, cetearyl alcohol, stearyl alcohol, and behenyl alcohol. The nano-emulsion of the weight ratio of (A) / [(B) + (C)] is 0.5 to 9, the average size of the emulsified particles is 100 to 1000 nm. The nano-emulsion of claim 1, wherein the weight ratio of the vitamin A or a derivative of vitamin A / acetyl hexapeptide-3 is 10,000 to 1. According to claim 1, wherein the vitamin A or a derivative of vitamin A nanoemulsion is included in 0.0001 to 10.0 parts by weight based on 100 parts by weight of the total composition. The nanoemulsion of claim 1, wherein the oil is included in an amount of 1 to 50 parts by weight based on 100 parts by weight of the total composition. The nanoemulsion of claim 1, wherein the nonionic surfactant comprises 0.5 to 20 parts by weight based on 100 parts by weight of the total composition. The nanoemulsion of claim 1, further comprising one or more selected from the group consisting of ceramides, ceramides 1 to 6, cholesterol, cholesterol esters, and cholesterol ethers. The nanoemulsion of claim 1, further comprising a wax in the oil phase as a viscosity modifier. The nanoemulsion of claim 1, further comprising a hydrophilic polymer in the aqueous phase as a viscosity modifier. The cosmetic composition for wrinkle improvement comprising 1-100 parts by weight of the nanoemulsion according to any one of claims 1 to 8. delete The cosmetic composition of claim 9, wherein the cosmetic composition is a lotion, essence, cream, wrinkle improvement. The nano-emulsion of claim 1, wherein the oil is selected from the group consisting of jojoba oil, liquid paraffin, and phenyltrimethicone.