KR101771125B1

KR101771125B1 - Methods for preparing medicinal herbs extract containing a high content of aroma components

Info

Publication number: KR101771125B1
Application number: KR1020150040159A
Authority: KR
Inventors: 구성민; 구태훈; 박규열; 정대화; 정철종; 윤칠석
Original assignee: (주)한의바이오; (주)옥천당
Priority date: 2015-03-23
Filing date: 2015-03-23
Publication date: 2017-08-28
Also published as: KR20160114229A

Abstract

The present invention provides a method for producing an oriental component-containing herbal extract and an oriental component-containing herbal extract. Since the process according to the present invention is carried out at a low temperature or at a normal temperature, it is possible to minimize the loss of a direction component mainly composed of a low molecular material in the manufacturing process. In addition, since the mineral-concentrated water is used as the extraction solvent in the present invention, the herbal medicine extract prepared according to the present invention contains various minerals (ionized calcium, magnesium, potassium, etc.) contained in the concentrated mineral water as well as aromatic components, These minerals can be absorbed directly into the body and contribute to health promotion.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for preparing a medicinal herb extract containing a high concentration of aromatic components,

The present invention relates to a method for producing an orient component-containing herbal extract and an orient component-containing herbal extract.

A Chinese medicine preparation is a medicine made by oriental medicinal materials according to oriental medicine principle. It is a prescription medicine made by combining herbal medicines appropriately for the treatment of pathologies, and is divided into a herbal medicine extract, a mixed extract and a combination extract.

The term "excipient" refers to a preparation made by granulating or powdering after extracting, concentrating, and drying one kind of medicinal material, and adding excipient to the granular powder or powder. The mixed excipient is prepared by mixing the prescription medicaments with each excipient and adding excipient Is a term used to mean a preparation made from granules or powders by mixing, extracting, concentrating, drying and adding excipients at a time.

Directional (fragrant) Herbals of Korean medicine include Korean ginseng, ginseng, gangjin, mackerel, broccoli, cinnamon, myrrh, cinnamon, cinnamon, mulberry, Baekdugwa, sandalwood, thyme, thyme, There are several kinds of fennel, mint, horseradish, horsetail, nutshell, clove, clove, clay, sea grass, The major oriental medicinal preparations that prescribe and prescribe the role of the oriental ingredient in the actual prescription of herbal medicines include acne, musk, octopus, fennel, peppermint, hyssop, hornblende, cinnamon, cinnamon,

As a general rule, the herbal preparation is inevitably subjected to a high-temperature spray drying process after concentrating a vacuum of 60 ° C in a vacuum at 60 ° C after extraction with hot water (extraction of the Korean Pharmacopoeia Standard for 2 to 3 hours) at 80-100 ° C, . In addition, the method of collecting the fragrance of the extract or essential oil in the prior art is a method of collecting the fragrance of the extract or essential oil by adding 10 to 60% of a hot water extract and pre-classified, cellulose, skimmed milk, dextrin, acacia gum and arabic gum, The microencapsulation method by spray drying was carried out so that a directional component was contained in the dried material. However, in the conventional method, a large amount of directional components are firstly lost in the hot water extraction process, and there is a problem that the directional components are lost secondarily in the spray drying process.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

DISCLOSURE OF THE INVENTION The present inventors have made efforts to develop a process for preparing a herb medicine containing oriental components at a high concentration in view of the problem that a directional component of herbal medicine is largely lost during the decompression concentration and high temperature spraying process after the conventional high temperature extraction. As a result, it is possible to minimize the disappearance of directional components in the extraction process by extracting at low temperature or room temperature using high-hardness mineral-concentrated water and capturing directional components using cyclodextrin, thereby producing a herbal medicine extract containing a high concentration of orienting components And the present invention has been completed.

Accordingly, it is an object of the present invention to provide a method for producing oriental component-containing herbal extracts.

Another object of the present invention is to provide an oriental ingredient-containing herbal extract.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention provides a method for preparing an oriental-containing herbal extract comprising the steps of:

obtaining a homogenization solution in which a mineral concentrate having a hardness of 50,000 to 400,000 mg / L and cyclodextrin are mixed and homogenized in the oriental ingredient-containing herbal medicine (a);

(b) extracting the orienting component from the orienting component-containing herbal medicine by placing the homogenizing solution obtained in step (a) under a temperature of 15-30 캜; And

(c) separating the liquid component from the result of step (b) to obtain a directional component-containing herbal extract.

According to another aspect of the present invention, there is provided an orient component-containing herbal extract extract prepared through the above-described method.

DISCLOSURE OF THE INVENTION The present inventors have made efforts to develop a process for preparing a herb medicine containing oriental components at a high concentration in view of the problem that a directional component of herbal medicine is largely lost during the decompression concentration and high temperature spraying process after the conventional high temperature extraction. As a result, the extraction of the orient component at low temperature or room temperature using the high hardness mineral concentrated water and the capturing of the orient component with the cyclodextrin were minimized, so that the oriental ingredient extract containing the orient component at a high concentration was minimized .

Hereinafter, each step of the present invention will be described in detail.

(a) Obtaining a homogenizing solution containing a directional component-containing herb medicine, mineral water with high hardness and cyclodextrin

In step (a), a homogenization solution obtained by mixing a mineral concentrate having a hardness of 50,000-400,000 mg / L and cyclodextrin in a herbal medicine containing an orienting component is obtained.

According to the present invention, the method of the present invention can be used without limitation as long as it is a herbal medicine (aroma component-containing herbal medicine) containing aroma components.

Examples of herbal medicines containing an orientable ingredient which can be used in the present invention include herbal medicinal herbs such as Ganoderma, Gammahang, Gangjin, Cinnamon, Mulberry, Myrrh, Sine, Creation, Zucchini, Baekduk, , Fructose, nutmeg, self-directional, clove, excess, civil flavor, salted, flavor, ointment, musk, octopus, fennel, peppermint,

According to one embodiment of the present invention, in step (a), crushed with orient component-containing herbal medicine is used. For example, the diameter of the pulverized orient component-containing herbal medicine may be 100-2,000 mu m. In one particular example, the milled orient component-containing herb has a diameter of 300-1500 mu m, 300-1000 mu m, or 300-600 mu m.

According to the present invention, a mineral concentrate having a high hardness (50,000-400,000 mg / L) prepared from salt ground water or deep sea water is used as an extraction solvent.

Sea water collected at 200 m depth below sea level is called deep sea water. Sea water and fresh water penetrate underground through underground rock, and sea water produced in underground rocks below 1000 m in coast is called salt groundwater. These seawater are rich in inorganic nutrients because they do not change their water temperature during the year and do not reach sunlight because they do not reach the sunlight. They are rich in minerals because they penetrate into the basement through rock masses. Especially, they contain essential trace elements such as magnesium and calcium, Contains a balanced balance of minerals. There is known a method of producing various mineral water with various hardness by conducting various known processes such as reverse osmosis filtration, electrodialysis, nanofiltration, heat concentration, etc. (Korean Patent Application No. 10-2008-0116464 number).

As used herein, the term "hardness" refers to the content of divalent metal ions (Ca ²⁺ , Mg ²⁺ , Sr ²⁺ , Fe ²⁺ , Mn ^2+, etc.) dissolved in water, Is expressed in mg / L unit as the amount of CaCO ₃ corresponding to < RTI ID = 0.0 >

According to one embodiment of the present invention, the hardness of the mineral concentrated water in step (a) is 100,000-400,000 mg / L.

According to one embodiment of the invention, the weight of the mineral-enriched water in step (a) is 2 to 12 times the weight of the orienting ingredient-containing herbal medicine. In one particular example, the weight of the mineral-enriched water is 2-10 times the weight of the orienting component-containing herbal medicine.

According to one embodiment of the present invention, the weight of the cyclodextrin in step (a) is 0.1-0.3 times the weight of the orienting ingredient-containing herbal medicine. In one particular example, the weight of the cyclodextrin is 0.1-0.2 times the weight of the orienting component-containing herbal medicine.

(b) Directional component - Extraction of directional component from herbal medicine

In step (b), the homogenization solution obtained in step (a) is placed under the temperature of 15-30 ° C to extract the direction component from the orient component-containing herbal medicine. Specifically, when the homogenization solution is left at a temperature of 15-30 ° C for a certain period of time, a directional component is extracted from the oriental herb-containing herbal medicine with a high-hardness mineral-concentrated water as an extraction solvent, and the extracted oriental component is extracted with cyclodextrin Lt; / RTI >

As described above, the present invention is differentiated from the conventional hot water extraction method in that it is carried out at a low temperature (for example, room temperature).

According to one embodiment of the present invention, the temperature in step (b) is room temperature (20-25 ° C).

According to one embodiment of the invention, the extraction in step (b) is carried out for 2-30 hours.

In the present invention, extraction can be carried out under a constant pressure to further improve extraction efficiency.

According to one embodiment of the invention, the extraction in step (b) is carried out under a pressure of 5-40 kg / cm < ^{2 &} gt ;. In one particular embodiment, the extraction pressure is ^{5-30 kg / cm 2, 5-25 kg} / cm 2 or 5-20 kg / cm ^2.

(c) Separation of liquid component (direction component-containing herbal extract)

When the step (b) is completed, the homogenizing solution contains mineral-concentrated water (liquid component) having high hardness containing directional components captured in the cyclodextrin and orient component-containing herbal medicine (solid component) ), The liquid component is separated from the result of step (b) to finally obtain the orient component-containing herbal extract.

The liquid component may be separated using a known method for removing solid components such as centrifugation and filtration.

According to the conventional method of processing herbal medicinal materials (Korean Pharmacopoeia herbal medicine standard), herbal medicine is put into an extractor, and 8-10 times of purified water is added thereto, and the mixture is extracted at a high temperature of 80-100 ° C for 2-3 hours. , And the filtrate is concentrated under reduced pressure at 60 ° C or lower, and is dried by spray drying or the like, and then the dried product is granulated. Such a conventional method has a problem that a large amount of aromatic components are lost in high temperature extraction, low pressure concentration and high temperature spraying.

In Oriental medicine, the stem of the cinnamon tree is called Cinnamon Bark, and Cinnamon Branch is the dried thing of cinnamon twigs. According to the pharmacopoeial standard, Cinnamon Branch Extract Powder should contain more than 81 ㎍ of 1 g of cinnamic acid as a directional component, 0.03% (300 ㎍) or more, and broiler extract powder which is extracted and dried by using a medicinal material conforming to the standard shall contain 81 ㎍ or more of cinnamic acid. Therefore, the disappearance rate of Shinnam acid by hot water extraction and spray drying is 73% even by simple calculation ignoring the surface component content and extraction efficiency of the raw material.

Therefore, in the following examples, the behavior of the direction components by the present invention and the conventional hot water extraction method were compared using cinnamon (broiler). The same weight of broilers was extracted from purified water or mineral concentrate (200,000 hardness), but the purified water was hydrothermally extracted at 100 ° C for 3 hours and the mineral water was extracted at room temperature for 3 hours after addition. The amount of cinnamic acid contained in the subsequent extract was measured and analyzed by high performance liquid chromatography. As a result, when the content of cinnamic acid contained in the pre-extraction cinnamon was 100, it was confirmed that 15% of cinnamic acid was contained in the hydrothermal extraction condition extract and 60% of cinnamic acid was contained in the mineral condensed water extraction condition (See Table 1).

(d) formulation

According to one embodiment of the present invention, the present invention further comprises the step (d) of formulating the orient component-containing herbal extract obtained in step (c) using an excipient.

Such formulation can be carried out by a conventional formulation method. For example, the oriental herb-containing herb extract obtained in step (c) may be added to the tableting excipient mixture in an amount of 5-20% by weight based on the unit weight of the herbal extract, followed by mixing into fine granules by a compaction machine, , Dried at room temperature for 6-12 hours, and then coated on the outside.

The features and advantages of the present invention are summarized as follows:

(I) The present invention provides a method for producing an oriental component-containing herbal extract and an oriental component-herbal extract.

(Ii) Since the process of the present invention is carried out at a low temperature or at a normal temperature, it is possible to minimize the loss of a direction component mainly composed of a low molecular material in the manufacturing process.

(Iii) In the present invention, since mineral concentrated water is used as the extraction solvent, the herbal medicine extract prepared according to the present invention contains not only aromatic components but also various minerals (ionized calcium, magnesium, potassium, etc.) contained in mineral concentrated water , These minerals can be absorbed directly into the body and contribute to health promotion.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

EXAMPLES Example 1. Comparison of extraction efficiency with the method of the Korean Pharmacopoeia (Chinese medicine) standard

Cinnamic acid (C ₆ H ₅ CHCHCO ₂ H, a slightly water-soluble white crystalline compound), which is a directional index component contained in broilers, is volatile and is processed by the processing method of short-lived herbal medicines specified in the Korean Pharmacopoeia Shin-Nam-san becomes a volcano.

In the conventional pharmacopoeial extraction method, 500 g of broiled chickens and 5,000 g of purified water (10 times of the raw material) were put in an extractor, and after reaching a center temperature of 100 ° C., they were extracted for 3 hours. The extract was squeezed at 10 kg / cm < ² > and again filtered through a 100 mu m filter to analyze the content of cinnamic acid.

Extraction with mineral-enriched water was first diluted with purified water to a hardness of 200,000 (mg / L) with mineral-concentrated water having a hardness of 380,000 (mg / L) (ARIBIA CORPORATION, the same applies hereinafter). Next, 500 g of broiled chickens and 5,000 g of mineral concentrated water having a hardness of 200,000 (mg / L) (10 times the raw material) were added to the extractor and extracted at room temperature for 3 hours. Compression and filtration conditions of the extracts were the same as those of the pharmacopoeial extraction. In the case of mineral - concentrated water treatment, the immersion time was added under pressure.

The Waters ACQUITY ™ photodiode array detector (PDA) and the HPLC column were packed with Waters ACQUITY ™ BEH C18 column (1.7 μm, 2.1 × 100) in a high performance liquid chromatography (UPLC; Waters ACQUITY ™ ultra performance LC system, USA) And the software was Empower. The reagents used were methanol (Junsei for HPLC), acetonitrile (for HPLC, JT BAKER) and water (third distilled water). Standard products were purchased from Sigma (USA). The results of the analysis are shown in Table 1.

Content of cinnamic acid per 1 ml of extract (%) Ratio of Extract Content to Broth Raw Material Shin-Nam-san Content (100%) Remarks RO-treated purified water extraction 0.003% 8.57% 3 hours extraction at room temperature Korean Pharmacy
Hot water extraction 0.005% 14.29% Extraction for 3 hours at 100 ° C Extraction of mineral concentrate ① 0.0215% 61.43% 200,000 (mg / L) hardness and 3 hours extraction at room temperature Extraction of mineral concentrate ② 0.0283% 80.86% Extraction after 24 hours of reaction at a hardness of 200,000 (mg / L) and 20 kg / cm ²

Cinnamic acid content in broilers is 0.035%. The content of cinnamic acid at the time of extraction was 0.005% by the method of the Korean Pharmacopoeia Specification. However, since the content of cinnamic acid at the extraction was 0.025% by the method described in the present invention, It was volatilized a lot. On the other hand, it was confirmed that the extraction effect of minerals with high hardness when compared with purified water under the same conditions minimized loss of directional components. In addition, when mineral water was used and high pressure was applied, it tended to be further improved when immersed at room temperature for 24 hours (Table 1).

Example 2. Degreasing and musk extraction

Soaking was very hard, so we used finely crushed powder in the grinder first. It is an iridescent tree with irregular lumps or irregularities or nicks on the resin of Aquilaria agallocha Roxburgh (red beech and Thymeleaceae), and its outer surface is dark brown, contains resin and many parallel fibers. When I burn it, I get a refreshing smell.

Musk is a dwarf musk curd Moschus berezovskii Flerove, hornblown Moschus chrysogaster Hodgson or musk herb Moschus moschiferus Linne (musk deer and Moschidae) male musculoskeletal secretion, which is taken out of its contents and dried, is called flour musk, and bag-shaped sand is cut and dried. Pocket musk is circular to elliptical, 3 ~ 7 cm in diameter, 2 ~ 4 cm in thickness and weighs about 10 ~ 30 g. Dried brown to reddish brown powder is a mixture of black and purple and sometimes black and purple grains.

These are very rare and the price of raw materials is expensive. Therefore, the range used in the actual prescription is 0.1% or less, which is used in special cases, and since it is used in a very small amount in actual use, it takes much time and attention to mix it sufficiently with other pharmaceutical powder to be uniformly distributed. In addition, in this embodiment, since the aromatic component is finally produced as fine granules having a particle size of about 100 탆, which is well dispersed and contained, it is convenient for uniform mixing with other medicinal materials.

(100 g) was pulverized in a pulverizer and 1,000 g of concentrated mineral water diluted to 200,000 (mg / L) was added thereto. The mixture was thoroughly stirred to prepare a homogenization solution, and a β- 20 g of cyclodextrin (CD) powder was dissolved in 200 g of purified water, followed by addition, homogenization, sealing, and reaction for 24 hours so that the directional component was sufficiently collected in the pores of β-CD. Then, 100,000 g of a 1: 1 mixture of lactose and corn starch was sealed in a mixing and pressure vessel and allowed to fully absorb for 24 hours while maintaining 15 kg / cm ² at room temperature (20-25 ° C). And then pressed sufficiently by using a roller compactor (Roller Compactor) to make irregular fine particles of 100 mu m in size. The microgranules were prepared in the same manner as in the case of musk dehydration.

Example 3. Extraction of Group I herb medicinal materials (mint, mokosei, mujangpyu, cremation, kwakae)

Before the extraction, the corresponding medicinal materials were finely pulverized and finely milled.

1000 g of the mint (or Group I individual herb medicinal materials) and 5000 g of mineral concentrated water diluted to 100,000 (mg / L) were added and stirred sufficiently to prepare a homogenization solution. The β-CD powder 150 g was dissolved in 1000 g of purified water and then added and homogenized. The mixture was sealed in a pressure-controlled vessel and reacted for 24 hours while maintaining the pressure at 10 kg / cm ² at room temperature (20-25 ° C) Minute. Then, the mixture was squeezed out and the squeezed material was filtered with a 100 μm filter cloth to obtain 5,250 g of the extract. And we got extracts of 5650 g of wheat germ, 4920 g of strawberry, 5150 g of gum, and 4120 g of gum.

Example 4: Extraction of Group II herb medicine (octagonal fennel, fennel, cinnamon, sine, loquat)

1,000 g of an octagonal fennel (or a second group of herbal medicines) and 2000 g of a mineral concentrate having a hardness of 380,000 (mg / L) were added and thoroughly stirred to prepare a homogenization solution. 100 g of? -CD powder corresponding to 10% Were dissolved in 1000 g of purified water and added and homogenized. The mixture was sealed in a pressure-controlled container and reacted for 24 hours while keeping the pressure at 5 kg / cm ² at room temperature (20-25 ° C) Respectively. Then, the mixture was squeezed out, and the squeezed material was filtered with a 100 mu m filter cloth to obtain 2200 g of the extract. Then, the lees were obtained by leaching method. 1710 g of fennel, 2650 g of cinnamon, 2050 g of sine and 1780 g of cooked liquor were obtained.

Example 5. Formulation

The basic compounding ratios of lactose, lactose, 15% corn starch, 15% corn starch, 3.0% povidone, 0.5% anhydrous silicic acid and 0.5% magnesium stearate were used.

5-1. Formulation of acne and musk

The tablets prepared in Example 2 containing 10% of fine fine grains were prepared by replacing 10% of lactose in the above basic blending ratio, that is, 10% of fine grained fine grains, 56% of lactose and corn starch, Respectively. Finally, 200 g of HPMC powder per 2.5 g of 2 mm sized granules were prepared in 2.5% aqueous solution and coated in a coater (Pan Coater).

5-2. Formulation of herbal medicines in Groups I and II

200 g of the prepared extract was used in an amount of 1000 g (basic blending ratio: lactose 66%, microcrystalline cellulose 15%, corn starch 15%, povidone 3.0 %, Anhydrous silicic acid 0.5% and magnesium stearic acid 0.5%), and then air-dried at room temperature and compressed to a size of 2 mm. Finally, 200 g of HPMC powder per 1000 g of 2 mm sized granules were prepared in 2.5% aqueous solution and coated in a fluidized bed coater.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims

A method for producing a cinnamon extract comprising the steps of:
(a) obtaining a homogenized solution in which a mineral concentrate having a hardness of 50,000 to 400,000 mg / L and a cyclodextrin are mixed and homogenized;
(b) extracting the homogenization solution obtained in step (a) under a temperature of 15-30 캜; And
(c) separating the liquid component from the result of step (b) to obtain a cinnamon extract.

delete

2. The method of claim 1, wherein the cinnamon in step (a) is ground to a size of 100-2,000 mu m in diameter.

The method according to claim 1, wherein the weight of the mineral-enriched water in step (a) is 2 to 12 times the weight of the cinnamon.

The method of claim 1, wherein the weight of cyclodextrin in step (a) is 0.1-0.3 times the weight of cinnamon.

The method according to claim 1, wherein the extraction in step (b) is carried out under a pressure of 5-40 kg / cm ² .

The method according to claim 1, further comprising (d) after step (c), formulating the cinnamon extract obtained in step (c) using an excipient.