KR101750893B1 - Zc4h2 유전자를 녹아웃시킨 형질전환 동물모델 및 이의 용도 - Google Patents
Zc4h2 유전자를 녹아웃시킨 형질전환 동물모델 및 이의 용도 Download PDFInfo
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- KR101750893B1 KR101750893B1 KR1020150079135A KR20150079135A KR101750893B1 KR 101750893 B1 KR101750893 B1 KR 101750893B1 KR 1020150079135 A KR1020150079135 A KR 1020150079135A KR 20150079135 A KR20150079135 A KR 20150079135A KR 101750893 B1 KR101750893 B1 KR 101750893B1
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| PCT/KR2016/003348 WO2016195224A1 (fr) | 2015-06-04 | 2016-03-31 | Modèle animal transgénique ayant gène zc4h2 inactivé, et son utilisation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190004663A (ko) | 2017-07-04 | 2019-01-14 | 고려대학교 산학협력단 | 우울증 또는 뇌전증 동물 모델과 그 제조방법 및 이를 이용한 우울증 또는 뇌전증 치료용 후보약물의 스크리닝 방법 |
| KR20190024658A (ko) | 2017-08-31 | 2019-03-08 | 고려대학교 산학협력단 | 조현병 동물모델 및 이의 제조방법 |
| KR20190116844A (ko) * | 2018-04-05 | 2019-10-15 | 재단법인대구경북과학기술원 | 뇌전증 동물 모델, 그의 제조방법 및 용도 |
| KR20200001022A (ko) * | 2018-06-26 | 2020-01-06 | 연세대학교 산학협력단 | Irx1 유전자-녹아웃 형질전환 제브라피쉬 모델 및 이의 용도 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019009586A1 (fr) * | 2017-07-04 | 2019-01-10 | 고려대학교 산학협력단 | Modèle d'animal dépressif ou épileptique, procédé d'établissement s'y rapportant et procédé destiné à cribler un médicament candidat destiné au traitement d'un trouble dépressif ou d'une épilepsie utilisant ledit modèle |
| KR102122500B1 (ko) * | 2018-03-07 | 2020-06-15 | 한국생명공학연구원 | Dyrk1a 유전자를 녹아웃시킨 형질전환 동물모델, 및 이를 이용한 신경 장애 관련 질환 약물 스크리닝 방법 |
| KR102328773B1 (ko) * | 2019-04-23 | 2021-11-19 | 고려대학교 산학협력단 | Fam19a5l 유전자를 녹아웃 시킨 제브라피쉬 동물모델 및 이의 제조방법 |
| CN111944835B (zh) * | 2019-05-14 | 2022-03-29 | 南通大学 | 一种突变型gabrg2转基因斑马鱼癫痫模型的构建方法及应用 |
| KR102271414B1 (ko) * | 2019-08-28 | 2021-07-01 | 충남대학교산학협력단 | Fam50a 유전자를 녹아웃시킨 동물모델 및 이의 용도 |
| CN111304251A (zh) * | 2020-03-12 | 2020-06-19 | 南通大学 | 一种斑马鱼难治性癫痫模型的构建方法及应用 |
| KR102612878B1 (ko) * | 2021-01-22 | 2023-12-13 | 충남대학교산학협력단 | eif2b3 유전자를 녹아웃시킨 동물모델 및 이의 용도 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20020187921A1 (en) * | 2001-04-04 | 2002-12-12 | Rubeinstein Amy K. | Transgenic zebrafish models for neurodegenerative disease |
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| KR100429397B1 (ko) * | 2000-08-28 | 2004-04-28 | 주식회사 티지 바이오텍 | 신경특이적 유전자 발현을 조절하는제브라피쉬의ＨｕＣ프로모터 유전자, 이를 이용한 형광성신경을 갖는 형질전환 동물 및 그의 제조방법, 그리고 이를 이용한 신경변이체 동물의 선별방법 |
| EP1855525A4 (fr) * | 2005-01-27 | 2010-04-28 | Zygogen Llc | Modeles poisson zebre de la maladie d'alzheimer |
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| US20020187921A1 (en) * | 2001-04-04 | 2002-12-12 | Rubeinstein Amy K. | Transgenic zebrafish models for neurodegenerative disease |
Non-Patent Citations (2)
| Title |
|---|
| Am. J. Hum. Genet., 92권, 5호, 페이지 681-695. (2013.05.02.).* |
| NCBI, GenBank Accession No. NP_955936.1, 'zinc finger C4H2 domain-containing protein [Danio rerio]', (2014.01.26.).* |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190004663A (ko) | 2017-07-04 | 2019-01-14 | 고려대학교 산학협력단 | 우울증 또는 뇌전증 동물 모델과 그 제조방법 및 이를 이용한 우울증 또는 뇌전증 치료용 후보약물의 스크리닝 방법 |
| KR20190024658A (ko) | 2017-08-31 | 2019-03-08 | 고려대학교 산학협력단 | 조현병 동물모델 및 이의 제조방법 |
| KR20200090664A (ko) | 2017-08-31 | 2020-07-29 | 고려대학교 산학협력단 | 조현병 동물모델 및 이의 제조방법 |
| KR20190116844A (ko) * | 2018-04-05 | 2019-10-15 | 재단법인대구경북과학기술원 | 뇌전증 동물 모델, 그의 제조방법 및 용도 |
| KR102109852B1 (ko) | 2018-04-05 | 2020-05-13 | 재단법인 대구경북과학기술원 | 뇌전증 동물 모델, 그의 제조방법 및 용도 |
| KR20200001022A (ko) * | 2018-06-26 | 2020-01-06 | 연세대학교 산학협력단 | Irx1 유전자-녹아웃 형질전환 제브라피쉬 모델 및 이의 용도 |
| KR102133179B1 (ko) | 2018-06-26 | 2020-07-13 | 연세대학교 산학협력단 | Irx1 유전자-녹아웃 형질전환 제브라피쉬 모델 및 이의 용도 |
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| WO2016195224A1 (fr) | 2016-12-08 |
| KR20160143913A (ko) | 2016-12-15 |
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