KR101739122B1 - Antibiotic composition having a wide scope of antibacterial activity untill drug resistance bacteria - Google Patents

Abstract

The present invention relates to an antibacterial composition exhibiting excellent and widespread antibacterial activity to antibiotic-resistant bacteria, such as super bacteria as well as general pathogenic strains. The antibacterial composition is manufactured by: injecting Lactobacillus plantarum to a mixture consisting of a dry Cornus officinalis leave powder, a dry Lycium chinense leave powder, a dry Lindera obtusiloba leaf powder, and a hive dried powder which completed honey extraction; culturing the mixture; and hot-water extracting the culture.

Description

[0001] The present invention relates to an antibacterial composition having antibacterial activity against bacteria having antibiotic resistance,

The present invention relates to a composition for antimicrobial activity having a wide range of antibacterial activity, and more particularly to a composition for antimicrobial activity having an excellent and broad antibacterial activity against antibiotic resistant bacteria such as super bacteria.

Direct or indirect damages caused by pathogenic microorganisms are causing economic, environmental and medical problems. The loss of food in the food industry due to corruption, the harmfulness and environmental pollution of the human body due to the use of excessive chemical pesticides in the agricultural industry, and the emergence of antibiotic resistant strains due to abuse of antibiotics. .

Vancomycin-resistant staphylococcus aureus (VRSA), a highly resistant strain of vancomycin, known as the last treatment for Staphylococcus aureus, the most common cause of human infection, was first introduced by the Centers for Disease Control in 2002 As a result, the possibility of so-called super-bacteria spread is increasing. This is the first time in Europe that vancomycin resistant enterococcus (VRE) resistant to vancomycin in 1988 after methicillin-resistant S. aureus (MRSA), which is only treated by vancomycin, And vancomycin intermediate-resistant S. aureus (VISA), a vancomycin resistant strain reported in Japan, the United States, France and Korea in late 1990, is a new example of antibiotic resistance that has emerged as a global crisis (Pfeltz, RF and Wilkinson, BJ 2004. The escalating challenge of vancomycin resistance in Staphylococcus aureus. Drug targets-infect. Disord. 4: 273-294. ; Levy, SB and Marshall, B. 2004. Antibacterial resistance worldwide: 122-129.).

Most of the currently used antibiotics are manufactured through chemical synthesis, which is costly and has many limitations such as causing side effects.

Therefore, in recent years, studies for separating new antimicrobial substances from natural products have been actively conducted. In order to isolate new antimicrobial substances from natural products, it should be considered that the antimicrobial spectrum is wide and safe even without long-term administration without adverse effects.

Korean Patent Publication No. 10-2013-0112322

Accordingly, an object of the present invention is to provide an antimicrobial composition exhibiting excellent antimicrobial activity against antibiotic resistant bacteria such as superbacteria, having a wide range of antimicrobial activity using natural products, especially plant extracts, free from toxicity and side effects.

In order to accomplish the above object, the present invention provides an antimicrobial composition having a wide range of antimicrobial activity, which is prepared by subjecting a mixture of dried corn oil leaf powder, dried gugija leaf powder and dried ginger leaf powder to hot water extraction.

In the antimicrobial composition having a broad antibacterial activity according to the present invention,

Preferably, the mixture further comprises a honeycomb dried powder having been densified. More preferably, the mixture is prepared by inoculating Lactobacillus plantarum to the mixture, culturing the mixture, and then subjecting the culture to hot water extraction.

In the antimicrobial composition having a broad antibacterial activity according to the present invention,

It is preferable that the weight ratio of the dried safflower powder, dried gugija leaf powder, dried ginger leaf powder and dried honeycomb dried powder is the same.

The antimicrobial composition having a broad antibacterial activity according to the present invention has antimicrobial activity against vancomycin resistant strain (VRE), methicillin resistant strain (MRSA), quinolone resistant resistant strain (QRSA) and multidrug resistant resistant strain (MDR).

The composition according to the present invention utilizes not only general susceptible strains but also multi-drug resistant bacteria such as super bacteria and exhibits excellent antimicrobial activity and also has no toxicity or side effects. It is used for preventing or preventing infectious diseases caused by pathogenic bacteria, antibiotic- And can be useful for treatment.

The present invention relates to an antimicrobial composition having broad antibacterial activity that exhibits excellent antimicrobial activity against antibiotic resistant bacteria such as superb bacteria as well as general pathogenic bacteria. The present invention relates to a composition for antimicrobial activity, which comprises a dry powder of corn oil, dried gugija leaf powder and dried ginger leaf powder The present invention provides a composition for antimicrobial activity having a wide range of antibacterial activity, which is produced by subjecting a mixture to hot water extraction.

The leaves of the acidulant oil used in the present invention are obtained by picking and drying the leaves of the acidulant oil. The leaves of the acidulant are not particularly limited. However, particularly preferred are the leaves of the spring, not the leaves of the autumn, The unoccupied collection from August uses dark leaves. These criteria are applicable to both Gugija leaf powder and ginger leaf powder.

Also, the ratio of the dried corn oil leaf powder, the dried Gujuga leaf powder and the dried ginger leaf powder to each other is not particularly limited, but it is preferable that they have the same ratio, and the ratio of water to the mixture in the hot water extraction, the time, Can be used without any particular limitation as long as it is well known in the art to which the present invention belongs.

Cornus wood in the present invention is the scientific name Cornus officinalis S. et Z. It is light brown with a height of 7m, but its bark is peeled off and faces oval, elliptical or ovate lanceolate. Length is 4 ~ 12㎝, width is 2.5 ~ 6㎝, surface is green, there is little bittern (伏 毛) and the back side is light green or white.

In addition, the peduncle is yellow in color and blooms earlier than leaves in April to April. Its shape is beautiful and it is cultivated much as ornamental water. The fruit is long elliptical and ripens in August. The fruit contains cornin, verbellin, tannin, ursohn, vitamin A, etc., and its pharmacological action is mild, poisonous, tasteful and sweet, and the kidney function and reproductive function deteriorate. It is effective when you take a look, a nightingale, a headache, tinnitus, waist and knee, and when you feel pain in the back.

Gugija wood in the present invention is the scientific name Lycium Chinense is a deciduous broad-leaved shrub belonging to the genus Chinense. Young leaves like a green leaf are edible and are also planted for ornamental purposes and distributed in Korea, China and Japan. The fruit is called gugija and it is used as a medicinal ingredient. It contains beta-in, beacaxanthin, carotene, thiamine, vitamin A, B1, B2 and C and it is used for chronic hepatitis, cirrhosis, It is used for lower back pain. Root skin is used as antipyretic agent such as peel, chill and so on.

In the present invention, the ginger tree has a scientific name Lindera obtusiloba I have Blume . It is obtusiloba and belongs to the camphoraceae . Its height is 3 ~ 4m, the leaves are 5 ~ 15㎝ long, 4 ~ 13㎝ wide and ovate or ovate round, It is a deciduous broad-leaved shrub that grows in the mountains of the country, which is round with the diameter of 0.7 ~ 0.8 ㎝, green to yellow or red, ripe in black, from around September to October. The leaves of the ginger tree are in the shape of a heart when they grow up, and the ginger-like fragrance comes from the stem, and the fragrance is strong even in leaves and flowers.

After mixing the above-mentioned leaves of Cornus sylvestris seed oil, leaves of Gujuga tree and leaves of ginger tree, the extract was extracted with hot water and used as an active ingredient. The antimicrobial activity of this extract was high, It is possible to make a composition.

In the present invention, it is preferable that the mixture of the leaves of Sanskrit tree, the leaves of Gugija, and the dry powder of ginger leaf further contains honeycomb dried powder which has been densified to have a more preferable effect in antibacterial activity and its application range do.

The honeycomb-dried powders that have been densely packed can be collected without any distinction between hanbong, beekeeping, The amount of dried corn oil leaf powder, dried Gugija leaf powder, dried ginger leaf powder and dried honeycomb dried powder is preferably the same ratio.

The honeycomb used in the present invention can not infiltrate with mold, virus, bacteria, etc. This is because of the healing substance called propolis called "natural penicillin", and the bees ask the honeycomb of the propolis and the saliva and the enzyme To the honeycomb inlet and outer wall to keep the inside of the honeycomb clean at all times

In order to increase the antimicrobial activity and extend the application range of the present invention, Lactobacillus plantarum is added to a mixture of dried leaves of corn oil, dried Gugija leaf powder, dried ginger leaf powder, And then cultured, and then the culture is subjected to hot-water extraction. As an active ingredient, an antimicrobial composition can be prepared.

Lactobacillus plantarum is a rod-shaped lactic acid bacterium with a high acid resistance generated in the late stage of kimchi fermentation. It is a bacterium that grows mainly when kimchi is fermented and sour, and has acid fastness and strong bite resistance .

Fermentation methods such as inoculation amount, fermentation conditions, and fermentation time in fermentation through inoculation of Lactobacillus plantarum can be used without any particular limitation as long as they are well known in the art to which the present invention belongs.

Hereinafter, the present invention will be described in detail with reference to the following Examples and Experimental Examples. However, the following examples and experimental examples are illustrative of the present invention, and the content of the present invention is not limited by the following examples and experimental examples.

Example  1. Preparation of the extract according to the invention

Dried corn oil leaves, dried Gugija leaves, and dried ginger leaves were each weighed into a grinder in an amount of 1 kg and pulverized to a size of not more than 30 mesh to obtain a dry powder, and then the dried powder was weighed Distilled water corresponding to 3 times was added and the mixture was reflux cooled for 3 hours at 100 ° C, filtered and concentrated under reduced pressure to obtain 600 g of an extract.

Example  2. Preparation of the extract according to the present invention

Dried corn oil leaves, dried ginger leaf, dry ginger leaf, and honeycomb which has been minced is put into a pulverizer in an amount of 1 kg each, and the pulverized powder is pulverized to have a particle size of 30 mesh or less to obtain a dry powder, Distilled water corresponding to the weight of the powder was added, and the mixture was refluxed at 100 ° C for 3 hours, cooled, and concentrated under reduced pressure to obtain 600 g of an extract.

Example  3. Preparation of the extract according to the present invention

Each 1 kg of dried corn oil leaves, dried Gugija leaves, dried ginger leaves and dense honeycombs were each pulverized in a pulverizer so as to have a particle size of 30 mesh or less to obtain a dry powder, and then the Lactobacillus planta (lactobacillus plantarum, KCCM11019P) and cultured. Three times of distilled water was added to the weight of the culture, and the mixture was refluxed and cooled at 100 ° C for 3 hours. After filtration and concentration under reduced pressure, 600 g of an extract was obtained.

Experimental Example  : Antimicrobial activity measurement of the extract according to the present invention

In order to examine the antibacterial activity of the extract of the present invention, the following experiment was conducted.

The test bacteria were cultured in Mueller Hinton broth and the antimicrobial activity was measured by broth microdilution. The test strains cultured up to the middle of the larval stage were put on a same medium so as to have a concentration of 10 ⁵ / ml, and 0.2 ml per well was added to a 96-well plate. The samples prepared in Examples 1 to 3 were diluted 2-fold Lt; / RTI >

And cultured at 30 DEG C for 18 hours to measure the growth of bacteria at 650 nm. The minimum concentration of compounds that completely inhibit the growth of bacteria was defined as MIC, and the results are shown in Table 1.

division Strain characteristic Example 1 Example 2 Example 3 Gram
Positive








Staphylococcus aureus CCARM 0203 sensibility 0.5 0.5 0.5 Staphylococcus aureus CCARM 0204 sensibility 0.5 0.5 0.5 Staphylococcus aureus CCARM 0205 sensibility 0.5 0.5 0.5 Staphylococcus aureus CCARM 3505 QRSA 1.7 1.3 1.0 Staphylococcus aureus CCARM 3002 MRSA 1.5 1.3 1.0 Staphylococcus aureus CCARM 3196 MRSA 1.7 1.3 1.0 Staphylococcus aureus CCARM 3579 MRSA 1.5 1.3 1.0 Enterococcus feacium CCARM 5212 sensibility 0.5 0.5 0.5 Enterococcus feacium CCARM 5024 VRE
(VanA) 1.6 1.3 1.0 Enterococcus feacium CCARM 5025 VRE
(VanB) 1.7 1.3 1.0 Gram
Negarive






Escherichia coli CCARM 0230 sensibility 0.5 0.5 0.5 Escherichia coli CCARM 0237 sensibility 0.5 0.5 0.5 Salmonella typhimurium CCARM 0240 sensibility 0.5 0.5 0.5 Pseudomonas aeruginosa CCARM 0225 sensibility 0.5 0.5 0.5 Klebsiella oxytoca CCARM 0249 sensibility 0.5 0.5 0.5 Escherichia coli CCARM 1136 MDR 1.7 1.3 1.0 Escherichia coli CCARM 1137 MDR 1.7 1.3 1.0 Pseudomonas aeruginosa CCARM 2009 MDR 1.7 1.3 1.0 Pseudomonas aeruginosa CCARM 2203 MDR 1.7 1.3 1.0 Salmonella typhimurium CCARM 8002 MDR 1.7 1.3 1.0

As shown in Table 1, the extract according to the present invention can be used as an antibiotic-sensitive strain of Gram-positive bacteria or Gram-positive bacteria, as well as vancomycin resistant strains (VRE), methicillin resistant strains (MRSA), quinolone resistant strains (QRSA) MDR) has also shown excellent antibacterial activity.

Examples of formulations for the composition of the present invention are illustrated below.

Formulation Example 1: Preparation of powder

0.1 g of extract

Lactose 1.5 g

Talc 0.5 g

The above ingredients were mixed and filled in an airtight container to prepare powders.

Formulation Example 2: Preparation of tablets

0.1 g of extract

Lactose 7.9 g

Crystalline cellulose 1.5 g

0.5 g of magnesium stearate

After mixing the above ingredients, tablets were prepared by direct tableting method.

Formulation Example 3: Preparation of capsules

0.1 g of extract

Corn starch 5 g

4.9 g of carboxycellulose

After mixing the above components to prepare a powder, the powder was filled in a hard capsule according to a conventional preparation method of a capsule to prepare a capsule.

Formulation Example 4: Preparation of injection

0.1 g of extract

Sterile sterilized water for injection

pH adjuster

(2 ml) per 1 ampoule in accordance with the usual injection preparation method.

Formulation Example 5: Preparation of a liquid preparation

0.1 g of extract

10 g per isomer

5 g mannitol

Purified water quantity

Each component was dissolved in purified water in accordance with a conventional method for producing a liquid agent, and the lemon flavor was added in an appropriate amount, followed by mixing the above components. Then, purified water was added to adjust the total volume to 100 ml, and the solution was filled in a brown bottle and sterilized to prepare a liquid preparation.

Claims (5)

Characterized in that the composition is prepared by hot extraction of a mixture of dried corn oil leaves, dried Goujita leaf powder, dried ginger leaf powder and honeycomb dried powder that has been compacted.
The antimicrobial composition according to claim 1, wherein the mixture is inoculated with Lactobacillus plantarum and cultured, and then the culture is subjected to hot water extraction.
The method according to claim 1,
Wherein the weight ratio of the dry powder of corn oil to the dried gugija leaf powder, the dry ginger leaf powder, and the honeycomb dried powder having been densified is the same ratio.
The method according to claim 1,
Wherein the antimicrobial activity is active against vancomycin resistant strains (VRE), methicillin resistant strains (MRSA), quinolone resistant strains (QRSA) and multidrug resistant strains (MDR).
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