KR101719101B1 - A root hairs specific promoter and use thereof - Google Patents

Abstract

The present invention relates to a promoter specific to root hairs of monocotyledonous plants, and a use thereof. The promoter of the present invention regulates expression of genes required for trait improvement for root hair, and thus can be useful for improving traits for root hair such as nutrient uptake through root hair in major monocotyledonous plants or crops including rice.

Description

Root-specific promoters and their uses {A root hairs specific promoter and use thereof}

The present invention relates to a root hair specific promoter of monocotyledonous plants and uses thereof.

Rice (Oryza sativa) is one of the most important food crops in the world. It has been steadily increasing its yields over the past two decades, but by 2020 it will have to produce more than 70% have. However, the agricultural land where rice is cultivated is getting smaller and smaller as the industrialization phenomenon of each country in the world, and the new genetic resource which is the subject of breeding is depleted and the introduction of the foreign useful gene is required. Fortunately, the development of molecular biology has enabled the isolation and manipulation of exogenous useful genes, transforming useful genes into many plant cells, including rice, to obtain transformants with new genes, Research has become a good source of breeding as well. The production of new varieties using this transformation will not only become a high value - added industry in the coming 21st century, but it will also solve some of the food problems that are highlighted as the greatest problems of mankind.

Many transgenic plants have been obtained by using polyethylene glycol (PEG) and electroporation in the protoplasts of the 1980s as a known transformation method. In the late 1990s, Agrobacterium, which has been widely used in the art, is also widely used. Other methods include pollen pathway and microinjection method.

The promoter is a genomic region connected to the upper side of the gene, and plays a role of regulating transcription of a structural gene linked to the promoter to mRNA. Since the proteins necessary for basic metabolism must maintain a certain concentration in the cells, the promoter linked to these genes is always activated by the action of a common transcription factor. Conversely, proteins that do not have a role in normal times and that require function only under specific circumstances may be activated by binding environmental transcription factors or specific transcription factors activated by external stimuli.

That is, a promoter can achieve the purpose of transformation by limiting the expression of a foreign gene to a whole body or a specific tissue of a plant as necessary, and may include the following promoters depending on function.

First, systemic expression-inducible promoters can be mentioned. As a plant systemic expression-inducing promoter, a promoter of 35S RNA gene of cauliflower mosaic virus (CaMV) is used as a typical promoter for dicotyledonous plants. Actin and maize ubiquitin gene promoters have been mainly used as promoters for the expression of the whole plants in rice plants and rice plants. Recently, a promoter of the rice cytochrome C gene (OsOc1) has been developed by domestic researchers, (Cf. Reg. No. 10-0429335). They are already inherent in inducing the expression of antibiotics, herbicide resistance genes and reporter genes used as selection markers in the plant transgenic carrier. From a research point of view, Promoters considered.

Second, seed-specific promoters can be mentioned. As a representative example, the rice glutelin promoter used for the development of golden rice as promoters of major storage protein genes of rice has been widely used to induce seed-specific expression of monocotyledonous plants to date, Promoter of soybean-derived lectin, napin promoter derived from Chinese cabbage, and gamma-tocopherol methyl transferase (gamma-TMT) in the seeds of Arabidopsis thaliana (Patent Application No. 10-2006-0000783) inducing seed-specific expression of carrot-derived DC-3 promoter and perilla originated oleosin promoter used in the study promoting the production of vitamin E by inducing gene expression, There is an example. The seed-specific promoters are mainly used for the purpose of accumulating useful proteins and producing beneficial substances in major crops in which seed itself is used as a food, a food, or a raw material for food.

Third, other tissue specific promoters such as leaves may be mentioned. (RbcS: ribulose bisphosphate carboxylase / oxygenase small subunit) promoter which induces expression of a strong gene only in photosynthetic tissues such as leaves, RolD promoter inducing expression of plant roots derived from Agrobacterium, potato-derived tuber A specific expression-inducible patatin promoter, and a tomato-derived fruit maturation-specific expression-inducing PDS (phytoene synthase) promoter.

Fourth is root specific expression promoter. (IbMADS) and sugar-induced adipose-glucose pyrophosphatase (AGPase) genes were isolated from the sweet potato-derived rice paddy rice, and the peroxidase (prxEa) (Korean Registration Nos. 10-0604186 and 10-0604191), confirming that the promoter induces specific expression in roots and induces root-specific transient expression in carrot and radish.

As described above, although a large number of promoters for specific expression are known, there is a need to develop promoters for further organ-specific expression.

For example, the root of the root is a cylindrical protruding part made from the epidermal cells of the root. Root hair effectively spreads the surface area of roots, plays an important role in absorption of water and nutrients, plant-microbial interactions, nitrogen fixation in the atmosphere, and rhizosphere. Considering the ability to absorb major nutrients, heavy metals and diffusion limited ions, root hair contributes greatly to agricultural, environmental and human health safety. That is, even in the root, there are genes to be expressed specifically in the root coat, and accordingly, there is a need to develop more specific promoters.

That is, a new promoter capable of regulating the location of the gene expression more precisely according to the intention of the developer, for example, a gene which should be expressed only in a specific organ (for example, a flower color, infertility, When switched, there is a need to continue to develop promoters that work only at specific institutions or periods.

Korean Registered Patent No. 10-0632486 2006. 9. 28. Korean Registered Patent No. 10-1383376 2014.4.2.

The present invention relates to a method for producing a recombinant vector, which comprises the step of expressing a foreign gene in a plant, a promoter capable of preferentially expressing the gene in the root of the plant, an expression vector containing the promoter, a transformant transformed with the expression vector, A composition for inducing preferential expression of a foreign gene comprising a foreign gene, a terminal plant transformed with the same, and a method for producing the same.

In order to achieve the above object, the inventors of the present invention have discovered that promoters capable of regulating plant tissue or organ-specific gene expression can be identified, thereby specifically expressing or inhibiting a gene of interest, And to establish a system with In this process, it was confirmed that the promoter of LOC_Os02g46500 of rice induces preferential expression in the root hair. Accordingly, the present invention has been accomplished by securing the promoter, preparing an expression vector containing the promoter, and then introducing the expression vector into a plant to confirm that the reporter gene is expressed preferentially in the root of the transformant.

In more detail, we performed agilent arrays using rice bulb roots and merged with published rice agilent data, GSE21494. GSE21494 is agilent data of 209 rice development step-by-step samples. It is a very high reproducibility that can be analyzed by 13 different tissues. Agilent array data from the roots of the present inventors also represent gene expression in root hair. Agilent arrays enable the analysis of gene expression expressed by 45151 probes in a single experiment. Through the analysis of the expression patterns of these data, genes expressed preferentially in the root hair were isolated.

Through this method, 409 genes showed higher expression patterns in root hair compared to other genes.

Regarding the promoter of LOC_Os02g46500 among the above genes, the promoter region from -1522 to +750 of LOC_Os02g46500 and the region including a part of the first exon were separated by genomic DNA PCR with reference to the base sequence decode information, and pGEM T- And cloned into an ejaculatory vector to decode the base sequence.

The thus isolated promoter was inserted into the pGA3519 vector to prepare a transformed plant. By verifying the GUS reaction in the transformed plants, the present invention was completed by inducing the expression of the root hair preferential gene of the plant using the corresponding promoter.

The present invention provides a root hair preferential expression promoter comprising the nucleotide sequence shown in SEQ ID NO: 1.

The root hair preferentially expressing promoter according to the present invention includes a promoter of LOC_Os02g46500 of rice, which is the nucleotide sequence of SEQ ID NO: 1, and preferentially expresses a foreign gene in root hair.

The promoter is an essential element to regulate the environmental, temporal, and systemic expression of the gene, and the expression site of the foreign useful gene in the plant can be preferentially induced in the root hair using the root hair preferential expression promoter according to the present invention. The present invention provides a promoter of LOC_Os02g46500 of rice and a promoter of a root hair preferential expression (hereinafter, referred to as 'promoter of the present invention') which induces expression of foreign genes preferentially to root hair.

In the present invention, "preferential" means a preferential expression increase in a part of a plant including all of the high specificity and specificity by the promoter according to the present invention. For example, 50% or more, 100% or more, 200% or more, 5-fold or more, or more, as compared with a plant lacking the promoter according to the present invention.

LOC_Os02g46500 encodes OsPUB36, an E3 ubiquitin ligase containing the U-Box domain, and consists of one exon.

The promoter of this LOC_Os02g46500 composed of 426 amino acids has the nucleotide sequence of SEQ ID NO: 1.

The invention also provides an expression vector comprising said promoter and an operably linked foreign gene.

The term "vector" refers to a DNA fragment (s), nucleic acid molecule, which transfers into a cell, which can be cloned and independently reprogrammed in host cells. "Expression vector" means a recombinant DNA molecule comprising a desired coding sequence and a suitable nucleic acid sequence necessary for expressing a coding sequence operably linked in a particular host organism. The expression vector may generally be derived from a plasmid or viral DNA, or may contain both elements. Thus, an expression vector refers to a recombinant DNA or RNA construct, such as a plasmid, phage, recombinant virus, or other vector that, upon introduction into an appropriate host cell, results in the expression of the cloned DNA. Suitable expression vectors are well known to those skilled in the art and include those that replicate in eukaryotic and / or prokaryotic cells and those that remain as episomes or that are integrated into the host cell genome.

Such a vector may be any vector as long as it can introduce the promoter of the present invention. Preferably, the vector may be a pCAMBIA family, a pGA family, a pGWB family such as pGA3519, pCAMBIA3301, pCAMBIA3300, pGA3426, pGA3780, pGWB12, pGWB14 Ti-plasmid, and vectors derived therefrom. These vectors contain the promoter and the foreign gene of the present invention and have a remarkable effect on expression of the foreign gene preferentially in the root hair.

The expression vector of the present invention includes a nucleic acid consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a functionally equivalent fragment thereof.

Means a fragment or a fragment of a nucleic acid consisting of the nucleotide sequence shown in SEQ ID NO: 1, which exhibits substantially equivalent effect to the promoter of the present invention. These nucleic acid fragments are 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95% 96%, 97%, 98%, 99% or more of sequence homology. Such a nucleic acid fragment can be easily prepared by molecular biological methods well known in the art. In addition, the promoter shown in SEQ ID NO: 1 of the present invention and a part of the gene region are located in front of LOC_Os02g46500 in the published rice genome sequence, and some of the nucleotide sequences shown in SEQ ID NO: 1 are substituted, inserted Deletion or deletion may be included in the scope of the present invention if the same homology with the promoter of LOC_Os02g46500 on the published rice genome sequence is maintained and the sequence located in some gene region exhibits substantially equivalent effect.

The expression vector of the present invention allows expression of a foreign gene for the target protein in the 3 'direction. Preferably, the expression vector may comprise a useful foreign gene that is preferentially expressed in the root hair.

For plants to grow, the absorption of nutrients and water is important and their role is dominated by root hair. In addition, it plays a role of fixing plants and synthesizing secondary metabolites. Thus, the foreign gene can be introduced whatever is related to the aforementioned function of the root hair and the characteristics of the root hair. For example, ROOT HAIRLESS1 , EXPA17, etc. of rice can be introduced as a gene related to root hair development. In addition, CSLD1 involved in the development of root hair and OsPT1 genes involved in nutrient absorption can be introduced, and examples of such foreign genes are not limited to the types of genes that can be introduced.

The above expression vector may be one in which the promoter of the present invention is introduced into a conventional vector, and the expression vector may be prepared by introducing the promoter of the present invention into such a manner as will be readily apparent to those skilled in the art. It is possible.

The present invention also provides a transformed cell transformed with said vector.

Such recombinant vectors may be introduced into host cells cultured using well known techniques such as infection, transfection, transfection, electroporation and transformation. Representative examples of the host include bacterial cells such as E. coli, Streptomyces and Salmonella typhimurium cells; And plant cells.

Any plant cell can be used as "plant cell" used for transformation of a plant. The plant cell may be any type of cultured cell, cultured tissue, cultured organ or whole plant, preferably cultured cell, cultured tissue or culture organ, and more preferably cultured cell. "Plant tissue" refers to a tissue of a differentiated or undifferentiated plant, such as, but not limited to, stem cells, leaves, cancer tissues, and various types of cells used in culture, such as single cells, protoplasts, Callus tissue.

The present invention also provides a composition for inducing preferential expression of the expression vector or a foreign gene comprising the transformed cell. The composition for inducing the primary expression of the root hair can induce the expression of the foreign gene preferentially in the root hair of the terminal leaf by introducing the foreign gene into the terminal leaf. In the present invention, the monocotyledonous plant includes, without limitation, all of the monocotyledonous plants including rice (Oryza sativa) according to one embodiment of the present invention. In the present invention, induction of preferential expression of the root hair means the preferential expression in the root hair of the terminal plant.

The present invention also provides a terminal plant transformed with the vector, the transformed cell or a composition for inducing preferential expression of the root of the transformed cell or a foreign gene comprising the same, wherein the terminal plant is preferably rice. These transgenic monocotyledonous plants exhibit the preferential expression of introduced foreign genes in the terminal plant root hair.

65%, 70%, 75%, 80% or more of the variants of the nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 "and" functionally equivalent transgenic plant of the present invention " , 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence homology As a terminal plant, it is a transgenic terminal plant having a characteristic that the promoter effect is substantially equivalent in the whole plant of the terminal plant.

Transformation of a plant of the present invention can be carried out by any method known in the art for transferring DNA to a plant. Such transformation methods do not necessarily have a regeneration and / or tissue culture period. Transformation of plant species is now common for plant species, including both terminal plants as well as dicotyledonous plants. For example, the calcium / polyethylene glycol method for protoplasts (Krens, FA et al., 1982, Nature 296, 72-74; Negrutiu I. et al., June 1987, Plant Mol. Biol. 8, 363-373) (Shillito RD et al., 1985 Bio / Technol. 3, 1099-1102), microinjection into plant elements (Crossway A. et al., 1986, Mol. Gen. Genet. (Klein et al., 1987, Nature 327, 70), infiltration of plants or mature pollen of various plant elements (DNA or RNA-coated) And infection by (non-integrative) viruses in Agrobacterium tumefaciens mediated gene transfer (EP 0301316). Such mutants can be easily produced by molecular biological methods well known in the art.

The present invention also encompasses a step of transfecting a monocotyledonous plant with a vector comprising a promoter comprising the nucleotide sequence of SEQ ID NO: 1 and a foreign gene operably linked thereto, and expressing the foreign gene in the monocotyledonous plant Which expresses a foreign gene in the root of the plant.

Preferably, the method comprises the steps of: preparing a composition for inducing preferential expression of a terminal plant of a terminal plant of a foreign gene comprising the expression vector or the transformed cell; Introducing said composition into a terminal plant; And selecting the transgenic monocotyledonous plants to which the composition is introduced and in which the foreign gene is preferentially expressed in the terminal root of the monocotyledonous plant.

The promoter of the present invention is a promoter capable of preferentially expressing a foreign gene in root hair, and it can regulate gene expression necessary for improvement of root hair characteristic, and thus, it is possible to control the root expression of nutrients And can be usefully used for improvement.

Fig. 1 is a heat map showing the isolated gene showing preferential expression in the root hair and its expression pattern.
2 is a diagram showing a microarray result showing preferential expression in the root hair of the LOC_Os02g46500 gene.
Figure 3 is a diagram of a vector used for the production of GUS plants containing the LOC_Os02g46500 promoter and part of the gene.
Fig. 4 is a diagram showing the result of GUS staining showing that the LOC_Os02g46500 promoter in the transgenic plant expresses preferentially in the root hair.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following embodiments are only examples for helping understanding of the invention, and thus the scope of the present invention is not limited thereto.

< Example  1> Large-scale selection of rice genes preferentially expressed in root hair

In order to select genes expressing preferentially in the root hair, an agilent array was carried out using the root hair of rice, and an open microarray database NCBI GEO (http://www.ncbi.nlm.nih. gov / geo /) with the agilent data GSE21494. Using this, it is possible to analyze gene expression by 14 tissues / tissues including root hair.

The above analysis database is shown in Table 1.

Table 1. Database for gene expression analysis reconstructed by institution / organization

Using the K-means clustering analysis method, gene groups having high expression in the root hair of the database for gene expression analysis shown in Table 1 were isolated.

This was performed using the K-means clustering analysis method of MeV, a microarray data analysis software, and 409 genes having preferential expression in the root hair among the databases for expression / organization-specific expression analysis were isolated.

The results are shown in Fig.

As shown in Fig. 1, a gene which shows stronger expression toward yellow is closer to blue, and the expression becomes lower toward blue. Since the root hair is an organ contained in the root, the genes whose expression in the root hair is two times higher than the root are classified as the root-dominant gene even if they are expressed in the root. In other words, we identified 409 genes that showed preferential expression in root hair in 14 institutions / organization databases.

< Example  2> Analysis of tissue expression by microarray data analysis

The expression pattern of LOC_Os02g46500 gene was analyzed by microarray database analysis and the results are shown in FIG.

As shown in FIG. 2, it can be confirmed that the root tissue has a yellow color, indicating that the expression of the gene by the LOC_Os02g46500 promoter is preferentially in the root.

< Example  3> promoter for preferential expression of root hair Cloning

PCR was performed using the primers prepared to clone the promoter of LOC_Os02g46500 and the genomic DNA of Oryza sativa L. japonica cultivar-group. Template DNA was used at 100 ng and each primer was used at 5 pmol. PCR conditions were 5 min at 95 ° C; Repeating 4 times at 95 캜 for 30 seconds, at 56 캜 for 30 seconds and at 68 캜 for 300 seconds; 30 seconds at 95 ° C, 30 seconds at 62 ° C, and 300 seconds at 68 ° C for 35 times and finally 68 ° C for 10 minutes. The PCR product was electrophoresed to confirm its size and cloned into a pGEM T-EG vector and sequenced. The cloned DNA was digested with Hpa1 and Xba1, followed by ligation with a pGA3519 vector (vector for promoter-GUS cloning) digested with the same restriction enzyme at 14 DEG C for 12 hours. The ligation product was mixed with 200 [mu] l of Top10 E. coli competent cells, transferred to a 1.5 ml tube, and left on ice for 15 minutes. Subsequently, the plate was allowed to stand in a 37 ° C oven for 1 minute, and then 1 ml of LB liquid medium was further placed in the tube and left in a 37 ° C shaking incubator for 3 hours. Then, the cells were plated on tetracycline-resistant LB solid medium and the resulting colonies were awaited for 12 hours and then mini-prepared after cell culture in 1 mL of LB liquid medium. The DNA obtained as miniprep was digested with restriction enzymes Hpa1 and XbaI, and the agarose gel was separated by electrophoresis and the band was confirmed. The cloning DNA thus obtained was subjected to base sequence analysis again to select DNA that did not cause an error. Nucleotide sequencing was performed by capillary sequencing (Macrogen), and primers using NGUS1 and promoter sequences were used for the nucleotide sequence analysis.

Primers for promoter cloning and primers used for base sequence analysis are shown in Tables 2 and 3 below.

Table 2. Primers for cloning promoter of LOC_Os02g46500

Table 3. Primers for sequencing

The sequence analyzed in the above is shown in SEQ ID NO: 1, and a promoter region from -1522 to +750 of LOC_Os02g46500 and a region including a part of the first exon were used as a promoter sequence.

< Example  4> Production of transformed cells

The plant expression vector prepared in Example 3 was extracted.

50 아 of Agrobacterium tumefaciens LB4404 and 2 의 of plant expression vector were mixed and left on ice for 15 minutes. Subsequently, the substrate was placed in liquid nitrogen for 75 seconds. Then, the substrate was allowed to stand in an oven at 37 ° C for 5 minutes. Then, 1 ml of LB liquid medium was added thereto, followed by incubation in a 28 ° C shaking incubator for 3 hours. Then, the cells were plated on a tetracycline-resistant LB solid medium and incubated for 36 hours. The resulting colonies were cultured in 1 mL of LB liquid medium, mini-preparations were performed, and Hpa1 and Xba1 were used to confirm the size after enzymatic cleavage.

The resulting transformed Agrobacterium was used for transgenic rice transformation experiments.

< Example  5> Production of Transgenic Plants

In the N6D solid medium, Dongjinbyeon seeds were grown in a 28 ℃ growth chamber for 7 days to produce calli of rice. The resulting callus was mixed with the cells cultured for 72 hours with Agrobacterium transformed with the plant expression vector obtained in Example 3, and then placed in a culture medium containing N6D-Acetosyringone at a temperature of 22 占 폚.

The callus contaminated with Agrobacterium was rinsed 5 times with tertiary distilled water. Subsequently hygromycin selection was performed in N6D solid medium (hygromycin 30 mg / L) and subculture (hygromycin 40 mg / L) . Selection was made in the 28 ℃ growth room for 2 weeks each for 4 weeks. The divided callus was transferred to the MSR (hygromycin 40 mg / L) solid medium for regeneration and induced to regenerate in a light condition growth chamber for 4 weeks at 28 ° C. The plants were transferred to MS solid medium, grown in a light condition growth room for 7 days, To grow regenerated plants.

< Example  6> Validation of predominant expression of root hair using transgenic plants

In order to confirm the organ specific GUS expression of regenerated plants, 10 seeds harvested were cultured at 28 ° C for 7 days and all individuals were stained with GUS.

The GUS solution was prepared by mixing 200 ml of 0.5 M sodium phosphate buffer, 100 ml of 12.5 mM potassium ferricyanide, 100 ml of 12.5 mM potassium ferrocyanide, 20 ml of 0.5 M EDTA, 50 ml of 10% Triton X-100, 1 g of X-glucin dissolved in 10 ml of DMSO, 200 ml of distilled water was added to make 1 L volume. Each plant was treated with 15 ml of GUS staining solution for 24 hours, followed by decolorization at room temperature using 70% ethanol and 100% ethanol sequentially.

The results are shown in Fig. Fig. 4 (A) shows plant roots on day 7 after germination, and Fig. 4 (B) shows photographs of root hair closely observed.

As shown in FIG. 4, it was confirmed that the transgenic plants showed predominant GUS expression in root hair, unlike GUS expression in other regions.

Based on the results of FIG. 2 and FIG. 4, it was confirmed that the promoter of LOC_Os02g46500 was preferentially expressed in root hair.

That is, it was confirmed from the above analysis result that the sequence including the promoter region from -1522 to +750 and the part of the first exon of the gene of LOC_Os02g46500 could act as a root hair preferential expression promoter.

<110> University-Industry Cooperation Group of Kyung Hee University <120> A root hairs specific promoter and use thereof <130> P16-053-KHU <160> 5 <170> KoPatentin 3.0 <210> 1 <211> 2272 <212> DNA <213> Oryza sativa <220> <221> promoter &Lt; 222 > (1) .. (2272) <400> 1 aagtgacact ctgagattac aaatgtgtgc actcaattct gaaactgcat gcttgttcca 60 accggatttc ccagctcaat tcaactttca accgtttgat ctgaaatgga tagttgcaac 120 cgaactccat aagattgacc tgcagtcatc tatgtaatcc attcatcccc agtttcaaag 180 tcattgcaaa gatcaggtaa accttgtaac ggaggaatcc caagatgaag atatcaaatc 240 tttgagatac tctagcctat ggttggatct ttaggtaaca agaaatttac aggagaaatc 300 acttgtacga ctagcacgat ctcttggcag attagtacga agcatctgat gatatattat 360 aatggcttta cgatcggtga taattcttag gatgcatttt atatgtgcat tagtcaacgt 420 ctggaatgat tcttagcgat catgagctgc gtttaaatag tcctttgata aatttttttt 480 caaggatttg aaagccatct gcttgtgacg tacctactgg ctactgagat acgaatcgca 540 ttacagggtc aatgcgaaca tgatctgaga ggtatagtgc gttccatgta tacttatgca 600 tcctcagatc agagagtttt tctattggtg tattgcacgt tagctcagcg tacacaattg 660 tgcaaacaaa acataatcac ctttctaatt attttcgtct gcatcactgc aaagacgttt 720 gagattttca gtacggaatc cgttcttgaa aggaaatggt atcttgttct tgacttgtaa 780 atgttaagaa ctttcggcgt ttgacttgag caagtggtgg tatcttactt gtttactccg 840 aggagcttag agcgcgcgcg tctcacttct aggacaggaa acatcgtcgt gcgaagcgct 900 caaccgcctg caactaaaat catatgaact gtaagcaaaa gagttcaaca atatacagcc 960 ttgcaagaga aaagcatgaa aatccttaaa aatcaacacc aataatcgga agatatctgc 1020 cgtccacacc caaagtgtct gccgtctcca tttaacaatt catgatatgc atatcacatt 1080 tgtcattttc ttatgttttt tgttttaaat taataccatg catattgcat agtctcctcc 1140 taactactcc agtaaaattt aattagtgga gatattagga ttgcacttgg tcatcatttg 1200 tcaagtatgg ccacaaacat ttccaatttc catcagattt atggatgtgg catggcatac 1260 atatctcgaa aaatactaca taatactata tactatgttt tagaaaatgg aaatgttttg 1320 cacgtcactt gaaaaagtcc aatttacagg tcacatcaag cttccatgag accatgacct 1380 tctcaccact aattgcaagc atctcttacc tttgactatt gacttgcaca cttgccagcc 1440 catatgcccc tacaagtata tataatgagc ccaccgtgtc attacttgct cagcttcagc 1500 tctgtgactg cgacgcgaac ccatggccgc catggacgag cctcctcagc tattcctctg 1560 cccgatctcc atggagctca tggaggatcc ggtgacggtg tccaccggcg tgacgtacga 1620 ccgccggagc atcgaggagt ggctcttcgt ctacggccgg accacctgcc cggccaccat 1680 gcagcctctc tccaacttcg acctcactcc caaccacact ctcaagcgcg tcatctcgtc 1740 gtggctcgat cgcggctcgt cgtcgtcgtc gtcttcgtcg ccgtcgacgt cgacgctgtc 1800 gagcccgatc cacgagctgg cgacgccgct gtcgcgcgcg ctggagcagg agcggttgct 1860 ggctgcgctg gccgagctgg aggagacgcc gttcaaggtg accaagctga agagcatgag 1920 ggcccgcatg gcgggcgacg tggcaatgca gggggagttc gtcgcctcgg gcggggtgcg 1980 cgtggtgggg cgcgtgatgg cgcaggcgct ggcggagagc ggcggcgact tctcggcgtt 2040 cgccgcgtgc gaggaggctg ccgcggtgct cgccgcgctc ccgctctcgg acgaggcgtc 2100 ggtgcgggtc gtccttgcgc cggagtgcat caggcccgtc atggcgctgc tccagcgcgg 2160 cggcgccgag gcgaggctgc acgccatgga catcctcacc aagatctcca gctccggatc 2220 aggcggcgac tggaccgccg gcgtcgacat cgacgacgtg atcaagtccc tc 2272 <210> 2 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Os02g46500 proF hpa1 <400> 2 gttaacaagt gacactctga gattac 26 <210> 3 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Os02g46500 proR XhaI <400> 3 tctagacgga gggacttgat cacgtcgt 28 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> NGUS1 <400> 4 aacgctgatc aattccacag 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Os02g46500 seq1 <400> 5 cttcagctct gtgactgcga 20

Claims (8)

1. A composition for inducing preferential expression of a foreign gene in a root, comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a vector comprising an operably linked foreign gene. 1. A composition for inducing preferential expression of a foreign gene in a root, comprising a transformed cell transformed with a vector comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a foreign gene operatively linked thereto. The composition according to claim 1 or 2, wherein the induction of the primary expression of the root is directed to the primary expression of the root of the rice (Oryza sativa). A transgenic terminal plant transformed with a composition for inducing preferential expression of a foreign gene according to claim 1 or 2 and expressing the foreign gene preferentially in the root hair of a terminal plant. The transgenic plant of claim 4, wherein the terminal plant is rice (Oryza sativa). Comprising the step of transfecting a monocotyledonous plant with a vector comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a foreign gene operatively linked thereto, and expressing the foreign gene in the monocotyledonous plant, A method for producing a transgenic monocotyledonous plant which is preferentially expressed in root hair. A method for producing a transgenic monocotyledonous plant wherein the foreign gene is preferentially expressed in the root of the monocotyledonous plant comprising the steps of:
Preparing a composition for inducing preferential expression of a foreign gene of a foreign gene, the vector comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a foreign gene operatively linked thereto;
Introducing said composition into a terminal plant; And selecting the transgenic monocotyledonous plants to which the composition is introduced and the foreign gene is preferentially expressed in the root of the monocotyledonous plant. 8. The method according to claim 7, wherein the terminal plant is rice (Oryza sativa).

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