KR101639880B1 - A seed preferential promoter in monocotyledones and use thereof - Google Patents

Abstract

The present invention relates to seed-preferential promoters of monocotyledonous plants and their uses.
The promoter of the present invention is a promoter that regulates gene expression necessary for improvement of the trait of rice seeds to improve the seed composition of the main cereals including rice and increase the tolerance to environmental stress of seeds, Lt; / RTI >

Description

A seed preferential promoter in monocotyledones and use thereof,

The present invention relates to seed-preferential promoters of monocotyledonous plants and their uses.

Rice (Oryza sativa) is one of the most important food crops in the world. It has been steadily increasing its yields over the past two decades, but by 2020 it will have to produce more than 70% have. However, the agricultural land where rice is cultivated is getting smaller and smaller as the industrialization phenomenon of each country in the world, and the new genetic resource which is the subject of breeding is depleted and the introduction of the foreign useful gene is required. Fortunately, the development of molecular biology has enabled the isolation and manipulation of exogenous useful genes, transforming useful genes into many plant cells, including rice, to obtain transformants with new genes, Research has become a good source of breeding as well. The production of new varieties using this transformation will not only lead to a high value-added industry in the coming 21st century, but will also solve some of the food problems that are the biggest problem of humanity.

Many transgenic plants have been obtained by using polyethylene glycol (PEG) and electroporation in the protoplasts of the 1980s as a known transformation method. In the late 1990s, Agrobacterium, which has been widely used in the art, is also widely used. Other methods include pollen pathway and microinjection method.

The promoter can achieve the transformational purpose by locating the expression of the foreign gene only at the whole body of the plant or a specific tissue, and can be classified as follows according to its function.

First, systemic expression-inducible promoters can be mentioned. As a plant systemic expression-inducing promoter, a promoter of 35S RNA gene of cauliflower mosaic virus (CaMV) is used as a typical promoter for dicotyledonous plants. Actin and maize ubiquitin gene promoters have been mainly used as promoters for the expression of the whole plants in rice plants and rice plants. Recently, a promoter of the rice cytochrome C gene (OsOc1) has been developed by domestic researchers, (Cf. Reg. No. 10-0429335). They are already inherent in inducing the expression of antibiotics, herbicide resistance genes and reporter genes used as selection markers in the plant transgenic carrier. From a research point of view, Promoters considered.

Second, other tissue-specific promoters such as leaves may be mentioned. (RbcS: ribulose bisphosphate carboxylase / oxygenase small subunit) promoter which induces expression of a strong gene only in photosynthetic tissues such as leaves, RolD promoter inducing expression of plant roots derived from Agrobacterium, potato-derived tuber A specific expression-inducible patatin promoter, and a tomato-derived fruit maturation-specific expression-inducing PDS (phytoene synthase) promoter.

Third, it is a root specific expression promoter. (IbMADS) and sugar-induced adipose-glucose pyrophosphatase (AGPase) genes were isolated from the sweet potato-derived rice paddy rice, and the peroxidase (prxEa) (Korean Registration Nos. 10-0604186 and 10-0604191), confirming that the promoter induces specific expression in roots and induces root-specific transient expression in carrot and radish.

Fourth, seed-specific promoters can be mentioned. As a representative example, captor motor of gluterin, prolamin, globulin and albumin genes, seed-storage proteins in rice, are used for expression of preferential gene expression in rice. The seed-specific promoters are mainly used for the purpose of accumulating useful proteins and producing beneficial substances in major crops in which seed itself is used as a food, a food, or a raw material for food. However, since all of these are promoters of the storage proteins, potential limitations in their application are anticipated. Therefore, in order to optimize a variety of endosperm-related traits, it is necessary to diversify the endogenous promoters necessary for its use.

In addition, although the development is currently underway, new promoters that can control the location of gene expression more precisely according to the intention of the developer, for example, genes that should be expressed only in specific organs (petals, roots, leaves, stems, Promoting activity to be performed only in specific organs or periods needs to be continuously developed when transforming plants, flowers, infertility, burning, specific metabolism-related substances, defensive substances, seed production and the like.

Korean Patent No. 10-1428631 Korean Patent No. 10-1369526

The present invention relates to a method for producing a recombinant vector, which comprises expressing a foreign gene by expressing the expression of the foreign gene locally to a non-systemic plant, an expression vector containing the promoter, a transformed cell transformed with the expression vector, A composition for inducing seed-preferential expression of a foreign gene, a transgenic transgenic plant, and a method for producing the transgenic plant.

In order to achieve the above object, the inventors of the present invention have found that promoters capable of regulating plant tissue or organ-preferential gene expression can be identified, thereby allowing preferential expression or inhibition of a target gene by locating it in a specific tissue or organ of a plant System. In this process, it was confirmed that the promoter of LOC_Os05g23910 of rice was not expressed in organs such as leaves and roots, and the expression was preferentially in seeds. Thus, the promoter was secured and an expression vector containing the promoter was prepared, Thus, the present inventors have completed the present invention by confirming that the reporter gene is preferentially expressed in the seeds of the transformant.

In more detail, we collected 1150 species of published rice affymetrix data and reclassified them into 17 organelles. The affymetrix array allows the analysis of gene expression represented by 57,000 probes in a single experiment and the organ-specific expression pattern produced by reconstruction of the large-scale collection of affymetrix arrays is quite high Reproducibility. Through analysis of the expression patterns of these data, various organ and tissue priority genes were isolated. Among them, the present inventors have been interested in finding promoters necessary for expressing important traits in seeds.

Through this method, more than 300 kinds of genes showed high expression patterns in seeds as compared with other genes.

The gene expression pattern regulated by these genes was precisely analyzed by expressing the GUS reporter gene in the 3 'direction of these promoters and measuring its activity in the plant.

With reference to the nucleotide sequence decode information, the ATG forward promoter region of LOC_Os05g23910 was isolated by genomic DNA PCR and cloned into pGEM T-Ease vector to decode the base sequence.

The thus isolated promoter was inserted into pGA3383 vector to prepare a transformed plant. By verifying the GUS response in the transformed plant, it was found that the promoter can be used to preferentially induce gene expression in the seed of the plant.

The present invention provides a promoter that preferentially expresses a foreign gene in a seed containing a promoter of LOC_Os05g23910 of rice, which is the nucleotide sequence shown in SEQ ID NO: 1.

A promoter is an essential element for regulating the environmental, temporal, and systemic expression of a gene. Using the seed-preferential promoter according to the present invention, the expression site of the foreign useful gene can be preferentially induced in the plant. According to one embodiment of the present invention, the promoter of the present invention is a promoter that preferentially expresses a foreign gene in the endosperm of seed.

The present invention provides a promoter of LOC_Os05g23910 of rice and a seed-preferential promoter (hereinafter, referred to as 'promoter of the present invention') which induces the expression of foreign genes to be preferentially in seeds.

LOC_Os05g23910 is expected to encode a histone-like transcription factor and archaeal histone, and is composed of one exon. The promoter of this gene consisting of 327 amino acids has the nucleotide sequence of SEQ ID NO: 1.

The invention also provides a vector comprising said promoter and operatively linked foreign genes.

The term "vector" refers to a DNA fragment (s), nucleic acid molecule, which transfers into a cell, which can be cloned and independently reprogrammed in host cells. "Expression vector" means a recombinant DNA molecule comprising a desired coding sequence and a suitable nucleic acid sequence necessary for expressing a coding sequence operably linked in a particular host organism. The expression vector may generally be derived from a plasmid or viral DNA, or may contain both elements. Thus, an expression vector refers to a recombinant DNA or RNA construct, such as a plasmid, phage, recombinant virus, or other vector that, upon introduction into an appropriate host cell, results in the expression of the cloned DNA. Suitable expression vectors are well known to those skilled in the art and include those that replicate in eukaryotic and / or prokaryotic cells and those that remain as episomes or that are integrated into the host cell genome.

Such a vector may be any vector as long as it can introduce the promoter of the present invention. Preferably, the vector may be a vector such as pCAMBIA family, pGA family, pGWB family such as pGA3383, pCAMBIA3301, pCAMBIA3300, pGA3426, pGA3780, pGWB12, pGWB14 Ti-plasmid, and vectors derived therefrom. These vectors contain the promoter and the foreign gene of the present invention, and have a remarkable effect on expression of the foreign gene preferentially in the seed. According to one embodiment of the present invention, the vector of the present invention is a vector that preferentially expresses a foreign gene in the endosperm of seed.

The expression vector of the present invention includes a nucleic acid consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a functionally equivalent fragment thereof.

Means a fragment or a fragment of a nucleic acid consisting of the nucleotide sequence shown in SEQ ID NO: 1, which exhibits substantially equivalent effect to the promoter of the present invention. These nucleic acid fragments are 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95% 96%, 97%, 98%, 99% or more of sequence homology. Such a nucleic acid fragment can be easily prepared by molecular biological methods well known in the art. In addition, the promoter represented by SEQ ID NO: 1 of the present invention is a sequence located in front of LOC_Os05g23910 on the published rice genome sequence. Even if some of the nucleotide sequences shown in SEQ ID NO: 1 are substituted, inserted or deleted, It can be included in the scope of the present invention if homology with the sequence located before the sequence LOC_Os05g23910 is maintained and exhibits substantially equivalent effect.

The expression vector of the present invention allows the gene for the target protein to be expressed in the 3 'direction. Preferably, the expression vector may comprise a useful foreign gene which is intended to preferentially express in the seed.

The foreign gene may be of any kind related to seed characteristics such as seed production, development, and the like. One of the most important traits of crops is the control of seed quality, which allows fine control and optimization of related traits by using preferentially expressed promoters in the seeds, especially in the endosperms that occupy the majority of seeds.

The above expression vector may be one in which the promoter of the present invention is introduced into a conventional vector, and the expression vector may be prepared by introducing the promoter of the present invention into such a manner as will be readily apparent to those skilled in the art. It is possible.

The present invention also provides a transformed cell transformed with said vector.

Such recombinant vectors may be introduced into host cells cultured using well known techniques such as infection, transfection, transfection, electroporation and transformation. Representative examples of the host include bacterial cells such as E. coli, Streptomyces and Salmonella typhimurium cells; And plant cells.

Any plant cell can be used as "plant cell" used for transformation of a plant. The plant cell may be any type of cultured cell, cultured tissue, cultured organ or whole plant, preferably cultured cell, cultured tissue or culture organ, and more preferably cultured cell. "Plant tissue" refers to a tissue of a differentiated or undifferentiated plant, such as, but not limited to, stem cells, leaves, cancer tissues, and various types of cells used in culture, such as single cells, protoplasts, Callus tissue.

The present invention also provides a composition for inducing seed-preferential expression of a foreign gene comprising the expression vector or the transformed cell. The composition for inducing seed preferential expression can specifically induce the expression of a foreign gene in a seed of a terminal plant by introducing a foreign gene into a terminal plant. In the present invention, the monocotyledonous plant includes, without limitation, all of the monocotyledonous plants including rice (Oryza sativa) according to one embodiment of the present invention. In the present invention, induction of seed preferential expression means preferential expression of a foreign gene in the seed of a terminal plant. According to one embodiment of the present invention, the composition for inducing seed-preferential expression of the present invention preferentially induces the expression of a foreign gene in the endosperm of seed.

The present invention also provides a transgenic plant transformed with the vector, the transgenic cell, or a composition for inducing seed-preferential expression of the transgenic cell comprising the transgenic cell or the transgenic cell. Preferably, the transgenic plant is rice. These transgenic monocotyledons exhibit the preferential expression of the introduced foreign gene in the terminal seed. According to one embodiment of the present invention, the introduced foreign gene of the present invention has the property that it is preferentially expressed in the endosperm of the terminal leaf seed.

65%, 70%, 75%, 80% or more of the variants of the nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 "and" functionally equivalent transgenic plant of the present invention " , 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or more sequence homology As a terminal plant, it is a transgenic terminal plant having a characteristic that the promoter effect is substantially equivalent in the whole plant of the terminal plant.

Transformation of a plant of the present invention can be carried out by any method known in the art for transferring DNA to a plant. Such transformation methods do not necessarily have a regeneration and / or tissue culture period. Transformation of plant species is now common for plant species, including both terminal plants as well as dicotyledonous plants. For example, the calcium / polyethylene glycol method for protoplasts (Krens, FA et al., 1982, Nature 296, 72-74; Negrutiu I. et al., June 1987, Plant Mol. Biol. 8, 363-373) (Shillito RD et al., 1985 Bio / Technol. 3, 1099-1102), microinjection into plant elements (Crossway A. et al., 1986, Mol. Gen. Genet. (Klein et al., 1987, Nature 327, 70), infiltration of plants or mature pollen of various plant elements (DNA or RNA-coated) And infection by (non-integrative) viruses in Agrobacterium tumefaciens mediated gene transfer (EP 0301316). Such mutants can be easily produced by molecular biological methods well known in the art.

The present invention also encompasses a step of transfecting a monocotyledonous plant with a vector comprising a promoter comprising the nucleotide sequence of SEQ ID NO: 1 and a foreign gene operably linked thereto, and expressing the foreign gene in the monocotyledonous plant A method for producing transgenic monocotyledonous plants expressing a foreign gene in seed preference is provided.

Preferably, the method further comprises the step of preparing a composition for inducing seed-preferential expression of a terminal plant of a foreign gene comprising the expression vector or the transformed cell. Introducing said composition into a terminal plant; And selecting the transgenic monocotyledonous plants to which the composition is introduced so that the foreign gene is preferentially expressed in the transgenic plant seeds.

The promoter of the present invention is a promoter that preferentially expresses seeds, and it is a promoter that preferentially regulates gene expression necessary for improving the trait of seeds to improve the seed components of major cereals including rice and to increase resistance to environmental stress of seeds Can be used to improve the agricultural traits of seed crops.

FIG. 1 is a diagram illustrating a process of selecting a promoter of a gene having high expression in a seed based on microarray data.
Fig. 2 is a diagram showing microarray results showing preferential expression in the seed of the LOC_Os05g23910 gene.
Fig. 3 is a diagram showing a diagram of a vector used for the production of the LOC_Os05g23910 promoter GUS plant.
Fig. 4 shows seed-preferential expression of LOC_Os05g23910 promoter in transgenic plants through GUS staining.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following embodiments are only examples for helping understanding of the invention, and thus the scope of the present invention is not limited thereto.

< Example  1> Large-scale selection of rice genes preferentially expressed in seeds

To select genes that preferentially express on the seeds, 1150 species of rice epimetrex microarrays (&quot; Nucleic Acid &lt; RTI ID = 0.0 &gt; affymetrix microarray) were reconstructed by 17 institutions / organizations to construct a database for gene expression analysis.

This is shown in Table 1.

Table 1. Database for gene expression analysis reconstructed by institution / organization

Using the K-means clustering analysis method, gene groups having high expression in the seeds of the database for gene expression analysis shown in Table 1 were isolated.

This was performed using MeV's K-means clustering analysis method for microarray data analysis. More than 300 kinds of genes having high expression in seeds, especially in seeds, were isolated from databases for expression analysis by tissue / tissue.

The results are shown in Fig.

As shown in Fig. 1, a gene which shows stronger expression toward yellow is closer to blue, and the expression becomes lower toward blue. That is, among the 17 reconstructed gene databases, about 300 kinds of genes showing preferential expression in the seeds, especially in the seeds, were identified.

< Example  2> Analysis of tissue expression by microarray data analysis

The expression pattern of LOC_Os05g23910 gene was analyzed by microarray database analysis and the results are shown in FIG.

As shown in Fig. 2, it can be confirmed that the endosperm of the seed is yellowish, indicating that the expression of the gene by the LOC_Os05g23910 promoter is preferentially in the seed.

< Example  3> Promoter for seed-preferential expression Cloning

SEQ ID NOS: 2 and 3 prepared for cloning the promoter of LOC_Os05g23910 were subjected to PCR using genomic DNA of Oryza sativa L. japonica cultivar-group as a template. The primer sequences are shown in Table 2. Template DNA was used at 100 ng and each primer was used at 5 pmol. PCR conditions were 5 min at 95 ° C; Repeating 4 times at 95 캜 for 30 seconds, at 55 캜 for 30 seconds and at 68 캜 for 300 seconds; 30 seconds at 95 ° C, 30 seconds at 62 ° C, and 300 seconds at 68 ° C for 35 times and finally 68 ° C for 10 minutes. The PCR product was electrophoresed to confirm its size and cloned into a pGEM T-EG vector and sequenced. The cloned DNA was digested with HpaI and SpeI, followed by ligation with pGA3383 vector (vector for promoter-GUS cloning) digested with the same restriction enzyme at 14 DEG C for 12 hours. The ligation product was mixed with 50 μl of Top10 E. coli competent cells, transferred to a 1.5 ml tube, and left on ice for 15 minutes. Subsequently, the plate was allowed to stand in a 37 ° C oven for 1 minute, and then 1 ml of LB liquid medium was further placed in the tube and left in a 37 ° C shaking incubator for 3 hours. Then, the cells were plated on tetracycline-resistant LB solid medium and the resulting colonies were awaited for 12 hours and then mini-prepared after cell culture in 1 mL of LB liquid medium. The DNA obtained as miniprep was digested with restriction enzymes HpaI and SpeI and then separated by agarose gel electrophoresis to confirm bands. The cloning DNA thus obtained was subjected to base sequence analysis again to select DNA that did not cause an error. Nucleotide sequencing was carried out using Macrogen (SEQ ID NOS: 4, 5, 6 and 7), and the corresponding primer sequences are shown in Table 3.

Table 2. Primers for cloning promoter of LOC_Os05g23910

Table 3. Primers for sequencing

The results of the analysis are shown in SEQ ID NO: 1, which corresponds to a promoter in which the approximately 2200 bp sequence preceding the ATG of the LOC_Os05g23910 gene is preferentially expressed on the seed.

< Example  4> Production of transformed cells

The plant expression vector prepared in Example 3 was extracted.

50 아 of Agrobacterium tumefaciens LB4404 and 2 의 of plant expression vector were mixed and left on ice for 15 minutes. Subsequently, the substrate was placed in liquid nitrogen for 75 seconds. Then, the substrate was allowed to stand in an oven at 37 ° C for 5 minutes. Then, 1 ml of LB liquid medium was added thereto, followed by incubation in a 28 ° C shaking incubator for 3 hours. Then, the cells were plated on a tetracycline-resistant LB solid medium and incubated for 36 hours. The resulting colonies were cultured in 1 mL of LB liquid medium. After mini-preparation, HpaI and SpeI were used to cleave the enzymes.

The resulting transformed Agrobacterium was used for transgenic rice transformation experiments.

< Example  5> Production of Transgenic Plants

In the N6D solid medium, Dongjinbyeon seeds were grown in a 28 ℃ growth chamber for 7 days to produce calli of rice. The resulting callus was mixed with the cells cultured for 72 hours with Agrobacterium transformed with the plant expression vector obtained in Example 3, and then placed in a culture medium containing N6D-Acetosyringone at a temperature of 22 占 폚.

The callus contaminated with Agrobacterium was rinsed 5 times with tertiary distilled water. Subsequently hygromycin selection was performed in N6D solid medium (hygromycin 30 mg / L) and subculture (hygromycin 40 mg / L) . Selection was made in the 28 ℃ growth room for 2 weeks each for 4 weeks. The divided callus was transferred to the MSR (hygromycin 40 mg / L) solid medium for regeneration and induced to regenerate in a light condition growth chamber for 4 weeks at 28 ° C. The plants were transferred to MS solid medium, grown in a light condition growth room for 7 days, To grow regenerated plants.

< Example  6> Seed-preferential expression test using transgenic plants

In order to confirm the seed-preferential GUS expression of the regenerated plants, some of the mature seeds were cut, and some of them were treated with GUS staining for 10 days at 28 ° C for 7 days.

The GUS solution was prepared by mixing 200 ml of 0.5 M sodium phosphate buffer, 100 ml of 12.5 mM potassium ferricyanide, 100 ml of 12.5 mM potassium ferrocyanide, 20 ml of 0.5 M EDTA, 50 ml of 10% Triton X-100, 1 g of X-glucin dissolved in 10 ml of DMSO, 200 ml of distilled water was added to make 1 L volume. Each plant was treated with 15 ml of GUS staining solution for 24 hours, followed by decolorization at room temperature using 70% ethanol and 100% ethanol sequentially.

The results are shown in Fig. 4. Fig. 4 (a) shows the results of GUS expression on the 7th day after germination, (b) shows the leaves, (c) shows the cross section of mature seeds, Show. As shown in FIG. 4, it was confirmed that GUS expression was dominant in the seeds, as opposed to very low GUS expression in other organs of the transgenic plants.

Based on the results of FIG. 2 and FIG. 4, it was confirmed that the promoter of LOC_Os05g23910 showed preferential expression in seeds. That is, it was confirmed that the sequence corresponding to about 2.2 kb in the front of ATG of the LOC_Os05g23910 gene can serve as a seed-preferential expression promoter through the above-described analysis results.

<110> University-Industry Cooperation Group of Kyung Hee University <120> A seed preferential promoter in monocotyledones and use thereof <130> P14-094-KHU <160> 7 <170> Kopatentin 2.0 <210> 1 <211> 2215 <212> DNA <213> Oryza sativa <220> <221> promoter &Lt; 222 > (1) .. (2215) <223> LOC Os05g23910 promoter <400> 1 ggttaacata ccctccaagt tgagatccct caaattccca tggaatgtct taatacaatt 60 gttcctatgt tcttgcatcc aatggtgaca aaactatttt ttgagagaaa acggtaacaa 120 catatggacc ctttcccccc tcccccaatt ctcactcaat ccctgcatta agaaaaaaaa 180 tggtaagaac agtttatacc tagactatac aaaataaatg ggaagatctt ggtactacaa 240 tgtgtccgtg ctattttact aaactagaag gtaccccgcg gcgttgctac ggaacattgt 300 agaaaaaaat tagaagaata gtttgtattg gaagtaaaag gattctaaag taagacaata 360 tgtgaatatt aatttgattg tatgataggg tacacaaaag ttttgttaga ctgaaaggga 420 actggcatat atcaaaatta tgattatttg aaagcaccta gagtatttag tttggacata 480 gtcttgacaa cggaaacagg atagaccatg attacgaaaa tagtaaacaa cggatgtgat 540 tattcggaag catctgcagt ctttaagctg tatattatgt attgacctgc aaatttcgct 600 ggacaatgta ttagaataaa tcataatcgg atttgcttgt atgatgaagc aaggcttcca 660 aacatgggtg attattaatg actaatttga tttgaactat tagatggatg atggattgat 720 ctaaggtgac caaacatggt ctacaatttt tgcttttatc tcttaaaaca tccgttacaa 780 cacacgtgcc tttatctagt tcttgttaat ttcaatcgat atgtatttgt tttttccatg 840 taaaatgtag tgaattggtt tttttagata aaaaatgaga ttttgaggga gagacgtcca 900 tccaaaaaat ttttaagaag aaattataag cgtgttgggg attgaacacg ggacctcgag 960 ttgaaaccat acatcccttg ccactgcact atcaagtgca tctctagtaa attggttttg 1020 agataaaaaa attgtagtat aattgttttt ttttgttttg gaaaccaata tcactactga 1080 ttcacccctt gaatcggcat atgatactga ccaacattgc cagttgaaca acacacaata 1140 ttaatgccta atttgatttg agctattaga tggatgatgg attgatctag ggtgaccaaa 1200 atcaggaaat gtgtattcca cagtcgtgct tcgcttaaaa tttaatcaga tagtcatgca 1260 ttgatgacaa cgtggaaagc tattatgaaa gagaatattt aatgctacat ggtgggaggc 1320 aattgtatag taatgcagga ttacaataca aggtaagata atttaagcct catttagatg 1380 ggctttattc tcagcacttc ggcccataaa aacaatgatt tagtgaaaaa caatatgcac 1440 cataacacat gtaaagtagg aaaagggctc aacatatgac ataccacgtt tggattgcag 1500 caaacaactt tattagttat gttgttacac aaaacaacac aaacaatccc ctcctctgct 1560 tacttacaac tgactatcaa tttctcaagc cttccagaag gctctttcta gcatgcggca 1620 aaatccatcg cttagcatgt acctgtacat tgataagcat tgagctagaa gaatcatcca 1680 aggatggcgt ggaggcttag accaccaagt atggaatgat gcataaaatt agtggatcgc 1740 ttgtacatca attcacaagt gtatggacat gcagtggtgc aaaaggaaag aagcaagaaa 1800 ggctaacaga accccgcaac aaacccacaa gagaacaatt agagacacct aacctcgatg 1860 ctttttcatg aatcatcgtg tctccagttg gcatccacaa tatcctttac tactttgtac 1920 tcctatataa actactttgc ttggttccca ttctctcatc gaccacacca taaattggtc 1980 acctgcttca acatcttgat ataagaaagg ttgtttatta tatcattatt atgtacttct 2040 actaattctt gagtgttagt gtaaattgtg ttagtggcta tacttccaac gagaagcaat 2100 gtagtttcta ttcaatttac atatttttac tgggaagatg aaataagttg ttgtttcaag 2160 caagtactta actgagttat tgttcatgca ttacttaaaa atgtaggagt aaaga 2215 <210> 2 <211> 28 <212> DNA <213> Artificial Sequence <220> <223> Os05g23910-pro_Hpa <400> 2 ggttaacata ccctccaagt tgagatcc 28 <210> 3 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> Os05g23910-pro_Spe1 <400> 3 gactagttct ttactcctac atttttaagt aatgc 35 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> NGUS1 <400> 4 aacgctgatc aattccacag 20 <210> 5 <211> 19 <212> DNA <213> Artificial Sequence <220> &Lt; 223 > RB_backbone-F2 <400> 5 tcgcacggaa tgccaagca 19 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Os05g23910-seq1 <400> 6 ggtgaccaaa catggtctac 20 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Os05g23910-seq2 <400> 7 gacctgcaaa tttcgctgga 20

Claims (9)

1. A composition for inducing seed-preferential expression of a foreign gene, comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a vector comprising an operably linked foreign gene. A composition for inducing seed-preferential expression of a foreign gene, comprising a transformed cell transformed with a vector comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and an operably linked foreign gene. 3. The composition according to claim 1 or 2, wherein the seed-preferential expression of the foreign gene is inducible in seed-preferential expression of a foreign gene, which is preferentially expressed in the endosperm of seed. The composition for inducing seed-preferential expression according to claim 1 or 2, wherein the composition for inducing seed-preferential expression is for inducing seed-preferential expression of a foreign gene in rice. A transgenic monocotyledonous plant transformed with a composition for inducing seed-preferential expression of a foreign gene according to claim 1 or 2, wherein the foreign gene is preferentially expressed in the seeds of the monocotyledonous plant. 6. The transgenic plant of claim 5, wherein the terminal plant is rice (Oryza sativa). Comprising the step of transfecting a monocotyledonous plant with a vector comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a foreign gene operatively linked thereto, and expressing the foreign gene in the monocotyledonous plant, A method for producing transgenic monocotyledonous plants expressing preferentially in seeds. A method for producing a transgenic monocot plant, wherein a foreign gene is preferentially expressed in the seed of the monocot plant, comprising the steps of:
Comprising the steps of: preparing a composition for inducing seed-preferential expression of a foreign gene, the vector comprising a promoter consisting of the nucleotide sequence shown in SEQ ID NO: 1 and a vector comprising an operably linked foreign gene;
Introducing said composition into a terminal plant; And selecting the transgenic monocotyledonous plants to which the composition is introduced so that the foreign gene is preferentially expressed in the transgenic plant seeds. 9. The method according to claim 8, wherein the terminal lobe is rice (Oryza sativa).

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