KR101710226B1 - Food composition containing the juice of Hippophae rhamnoides L. - Google Patents

Abstract

The present invention relates to a composition for reducing lipid or glucose comprising vitamin Hippophae rhamnoides L. juice, and more particularly to a composition for reducing vitamin, such as glucose, cholesterol and / or triglyceride ). Thus, it is possible to provide a composition capable of preventing or treating diabetes, liver disease and / or hyperlipemia and the like.

Description

[0001] The present invention relates to a food composition containing a vitamin-containing fruit juice,

The present invention relates to a composition comprising vitamin Hippophae rhamnoides L. juice, and more particularly to a composition comprising a vitamin tree having a function of reducing glucose, cholesterol and / or triglyceride of human body And is intended to provide a composition capable of preventing or treating diabetes, hyperlipidemia, liver disease and the like.

Vitamin tree (Hippophae rhamnoides L.; Sanjayu) is a plant of Eurasian origin and its scientific name is Hippophae rhamnoides L. The origin of this scientific name is a plant which has the scientific name of 'Rhamnoides L' which means 'Hippo' for 'horse' in Latin and 'Phae' for light. Hanja is called sandy spiky pole (spine), and it is also called SAJEE or SHAJI in tinnitus.

Vitamin tree is a deciduous woody plant that grows mainly in the dry or sandy areas, with sharp thorns on the branches. It grows well in the cold of winter and the high temperatures of summer, and has a strong adaptability that grows well in the barren zone. The name 'Sea buckthorn', the English name of the vitamin tree, is derived from the characteristic of having a habit of growing near the ocean and a lot of visibility. There are subspecies that grow to a height of 2 ~ 4m but grow from 50cm to 18m. There are a total of 12 subspecies, ssp.monglica of Siberian origin, ssp.rhamnoides of European origin, and ssp.sinensis of Chinese native species. It is widely distributed in mainland China, Russia and Europe, and about 90% is distributed in mainland China. It grows mainly in the plateau of 600 ~ 5,000m above sea level, and is distributed widely around the Himalayas (border areas of India and Pakistan), especially in the Inner Mongolia plateau and the clean plateau. From September to October, orange fruits with a diameter of about 5 mm are opened.

Medical records of vitamin trees were first recorded at the time of Chinese Tang Dynasty. The four-part medical history of the classic medicine book of Tibetan medicine details the use of vitamins in medicine. And, from 1271 to 1368, at the Kublai Khan of the Yuan Dynasty, vitamin Nut was used as a 'health product for the emperor'. The medicinal value and health management function was rewritten in the pharmacopoeia of the Pearl Material Medica of the Qing Dynasty from 1644 to 1911 and in 1977 the vitamin tree was recorded in the Chinese Pharmacopoeia, It is listed in the medical encyclopedia.

This vitamin tree is a shrub belonging to the barley family. It contains more than 100 kinds of ingredients, and it is known that it contains a lot of effective ingredients such as vitamins (A, B, C, E, F and K).

Therefore, various research fields such as measurement of antioxidant activity of vitamin tree, fatty acid content of vitamin tree, prevention of cancer and immunity system have been carried out, and it has been reported that beta-carotene is contained in vitamin Nut. In addition, we measure the active ingredient through the analysis of the vitamin tree leaf, and make various foods using vitamins.

In recent years, it has been actively cultivated in northern China and other countries as it has been spotlighted as a plant that prevents soil loss and saves the earth as a raw material for foods and pharmaceuticals, and active research is being conducted recently as a diet drug

In this regard, Korean Patent Publication No. 2010-0054772 (title of the invention: Vitamin tree extract and fractions thereof) describes an antioxidant containing vitamin tree leaves, root or stem extract or organic solvent fraction thereof as an active ingredient.

However, research on vitamin wood has not yet been actively conducted, and only a part of the antioxidant activity of vitamin tree has been confirmed.

Accordingly, there is always a need for studies on various useful ingredients using vitamin wood and development of medicines and food compositions using them.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems, and it is an object of the present invention to provide a composition capable of reducing glucose, cholesterol and / or triglyceride of a human body by using vitamin wood.

The present invention also provides a composition having a useful effect for preventing or treating diabetes, hyperlipemia, liver disease and the like by reducing glucose and / or lipid (neutral fat).

In order to accomplish the above object, the present invention is a composition for reducing lipid and / or glucose comprising a fruit juice of Vitamin tree (Hippophae rhamnoides L.).

The lipid may be at least one selected from the group consisting of cholesterol and triglyceride.

In addition, the present invention may be a composition for preventing or treating hyperlipidemia, liver disease and / or diabetes, including flesh pressured products of vitamin Nut.

In another aspect of the present invention, there is provided a method for preparing a flesh of vitamins, And compressing the prepared flesh to obtain a juice. The present invention also provides a method for producing a composition for reducing lipid and / or glucose.

The present invention also relates to a method for preparing a flesh of vitamins, Pressing the prepared flesh to obtain juice; Homogenizing the obtained juice at a pressure within a range of 20 to 40 MPa using a high-pressure homogenizer; Filtering the homogenized juice to obtain a liquid phase; Placing the obtained liquid phase in a bottle and sterilizing it in a vacuum state; Cooling the sterilized liquid phase; And a step of putting the cooled liquid phase in a container. The method for preparing a composition for reducing lipid and / or glucose may be used.

The details of other embodiments are included in the detailed description and drawings.

The present invention relates to a composition comprising vitamin-wood fruit juice, which has the effect of reducing the glucose, cholesterol and / or triglyceride of the human body.

In addition, the composition according to the present invention is characterized by reducing glucose and / or lipid, and is useful for preventing or treating diabetes, hyperlipidemia, liver disease and the like, and has an effect of decomposing lactic acid to generate an energy source.

FIG. 1 is a flow chart showing a process for preparing a composition for reducing lipid or glucose comprising vitamin wood nuts according to a preferred embodiment of the present invention,
FIG. 2 is a graph showing changes in glucose, cholesterol, and triglyceride of subjects to which the composition according to the present invention was administered.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and will be described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The terminology used in this application is used only to describe a specific embodiment and is not intended to limit the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the terms "comprises" or "having" and the like are used to specify that there is a feature, a number, a step, an operation, an element, a component or a combination thereof described in the specification, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

The terms first, second, etc. may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another.

The present invention relates to a composition for reducing lipid and / or glucose comprising a fruit juice of a vitamin tree (Hippophae rhamnoides L.).

The inventors of the present invention have been studying various physiological activities such as vitamins, leaves, stems and the like, and found that the fruit juice of the vitamin tree reduces the glucose, cholesterol and / or triglyceride of the human body The present invention has been completed.

That is, as can be seen from the examples described later, when the vitamin Nut fruit juice according to the present invention is consumed, glucose and cholesterol are decreased in all users and triglyceride is remarkably decreased in most users (Experimental Example 2 Reference).

For this purpose, vitamin trees available in the present invention can be used without limitation, such as cultivated or commercially available.

The present invention is characterized by using fruit among fruits, leaves and stems of a vitamin tree, and in particular, it is characterized by containing fruit juice. Vitamin Nuts can be obtained by removing leaves and stems from the Vitamin tree, and by removing the peel from the fruit thus obtained, you can obtain a grain or pulp, which can be squeezed to obtain juice. The juice may be a liquid component obtained from the nuts or flesh of the vitamin Nut fruit, which may include some grain or pulp. Since the pressed meat of the fruit is obtained by grinding or squeezing the lump or the flesh of the fruit, it may contain pulp and moisture, and the water contains about 70%.

The fruit juice of the vitamin tree not only contains vitamins together with fatty acids, but also contains a number of various essential amino acids, minerals, nutrients and the like (see Experimental Example 1). That is, the fruit juice of the vitamin tree according to the present invention contains about 600 to 700 mg (preferably 650 mg) of a fatty acid per 100 g, and about 150 to 11,000 mg (preferably 150 to 200 mg, more preferably 170 mg) of vitamin , Especially omega fatty acids and vitamins in large quantities. The present invention has different characteristics from the fruit juice of other fruits in that it does not often contain the two components together in one fruit. In addition, the oil-soluble components contained in the vitamin wood raw material have a high yield that is absorbed into somatic cells.

Thus, according to the present invention, a composition comprising fruit juice of a vitamin tree contains various useful components and has a characteristic of reducing glucose and / or lipid (cholesterol and / or triglycerides) and triglycerides. Accordingly, the composition according to the present invention can be useful for preventing or treating diabetes, hyperlipidemia, liver disease and the like.

FIG. 1 is a flow chart showing a process for preparing a composition for reducing lipid or glucose comprising a vitamins fruit juice according to a preferred embodiment of the present invention.

As shown in the figure, the method for preparing a composition for reducing lipid and / or glucose according to the present invention comprises: (S10) preparing peeled skin from vitamin Nut fruits; And pressing the prepared flesh to obtain juice (S20).

The step S10 of preparing the skin from which the skin is removed from the vitamin Nut fruit is for obtaining juice from the vitamin Nut fruit. For this purpose, the vitamins are first harvested, the fruit and stem are separated therefrom, and the stem is removed to obtain the fruit. It is then possible to separate the skin from the fruit (or flesh) from the fruit through a grinding process.

The step (S20) of compressing the prepared flesh to obtain a juice comprises a large amount of useful components from vitamin flesh, and is to produce a juice suitable for pharmaceutical or food composition. For this purpose, it is possible to squeeze the prepared flesh with a high-pressure squeezer to extract juice. After pressing, it is preferable to leave the oil component of the produced juice as it is. That is, the oil component is an (omega) fatty acid, and the vitamin tree fruit juice according to the present invention has both omega fatty acid and vitamin component, and contains many other useful components.

Next, the manufacturing method according to the present invention may further include a step (S25) of keeping the juice obtained as described above at a low temperature. For example, the juice can be stored by freezing rapidly at a temperature of minus 20 ° C, and thawing can be performed by heating at 50 to 55 ° C.

According to such a production method, it is possible to produce a composition containing fruit juice of a vitamin tree. Such a composition contains various useful components, in particular, glucose and / or lipid (cholesterol and / or triglyceride) It has the effect of reducing fat.

Meanwhile, the method for preparing a composition according to the present invention may further include homogenizing the obtained juice at a pressure within a range of 20 MPa to 40 MPa using a high-pressure homogenizer (S30). That is, homogenization process of high pressure is performed for fine homogenization of pulp. The high pressure homogenization is preferably carried out using a high pressure homogenizer well known in the art and is preferably performed at a pressure in the range of 20 to 40 MPa (preferably in the range of 25 to 35 MPa, more preferably about 30 MPa). The present inventors conducted experiments under various conditions using a high-pressure homogenizer, and found that the most homogeneous components were obtained when the pressure was in the range of 20 MPa to 40 MPa.

In addition, the present invention may further include a step (S40) of filtering the homogenized juice to obtain a liquid phase. That is, the homogenized juice is filtered to separate solid components and obtain only liquid components suitable for use as medicines and food compositions. For example, it is possible to filter the liquid with a 120 mesh filter net. The liquid component includes all the vitamins useful as vitamins, and may contain some pulp.

Subsequently, the present invention may further include a step (S50) of sterilizing the obtained liquid phase in a bottle and vacuum state. That is, since the vitamins nut juice according to the present invention is 100% natural juice and mold may occur, it is preferable to pass sterilization process to cut off the vitamins. For this purpose, the obtained liquid phase may be introduced into the automatic branching device and fed in. For example, the bottled juice is covered with a polyethylene material or a CAP with an inner cap and a steel cap of butyle rubber combined to prepare for sterilization in a vacuum state. When CAP is used, the inner cap and the steel cap of the polyethylene material or butyl rubber are combined with each other to prevent the generation of environmental hormones that may occur during pasteurization to the utmost. Then, the bottle containing the liquid phase can be sterilized for about 10 minutes by putting it in a high-pressure chamber in a vacuum state.

Then, the present invention may further comprise cooling the sterilized liquid phase (S60). For example, it is possible to rapidly cool a sterilized liquid phase in an automatic cooling water tank. Then, it is possible to conduct a microorganism test (total number of strains, coliform group, fungi and yeast group, PH, sugar content, total acidity test).

In addition, the present invention may further include a step (S70) of placing the cooled liquid phase in a container. This is to store the liquid component obtained as described above in a container and commercialize it. Storage is preferably tanned so that the sunlight transmittance is the lowest, and it is preferable to store in a shaded place in a glass bottle in which the container is not corroded because the acidity is low.

Vitamin tree juice prepared as described above can be directly commercialized and can be aged for commercialization for a predetermined period of time. In case of commercialization without aging, a fresh texture can be obtained, and a large amount of vitamin can be ingested. In the case of aging for 1 month to 1 year, preferably 4 months to 8 months, more preferably 5 months to 7 months, and inducing the enzymatic reaction and commercializing it, the absorption rate absorbed by the somatic cells can be increased, Do. That is, when the vitamins of the present invention are aged, the vitamins that are weak to heat or sunlight are destroyed by about 60%, but the remaining 40% of vitamins remain. This means that when vitamins are aged with other inorganic salts and fatty acids, The rate of uptake up to 90% is absorbed into the cells and is advantageous for cell activation.

In addition, the present invention may be a medicinal and / or food composition containing a vitamin wood fruit juice, which is produced by the above-mentioned production method. Such a composition has the effect of reducing glucose, cholesterol and / or triglycerides of the human body, and thus has a useful effect for the prevention or treatment of diabetes, hyperlipemia, liver disease and the like.

Furthermore, the present invention is also preferably a pharmaceutical preparation, composition or kit in the form of a lyophilizate or a liquid buffer or artificial breast milk preparation containing the fruit juice of the vitamin Nut. Such a pharmaceutical composition may comprise a peptide or peptide combination as an active ingredient together with at least one pharmaceutically acceptable carrier, excipient, binder, disintegrant, glident, diluent, lubricant, colorant, sweetener, flavoring, preservative, . The pharmaceutical compositions of the present invention can be prepared in conventional manner in conventional solid or liquid carriers or diluents and conventional pharmaceutically-acceptable adjuvants, at the appropriate dosage levels by known methods.

Preferably, the vitamin Nut fruit juice is suitable for intravenous administration, is suitable for oral administration, or is suitable for inhalation administration. Dosage forms include, for example, pills, tablets, film tablets, coated tablets, capsules, liposomal preparations, micro and nano preparations, powders, and deposits. The present invention also encompasses the use of a compound of formula (I) as described herein for the manufacture of a medicament for the treatment of a disease or condition selected from the group consisting of skin, intradermal, intragastric, intracutaneous, intravascular, intravenous, intramuscular, intraperitoneal, intranasal, intrathecal, intraoral, percutaneous, , Or transdermal administration, and the preparations contain, in addition to the typical vehicle and / or diluent, the juice of the vitamin Nutrition of the present invention.

The present invention relates to a pharmaceutical agent and / or a nutritional supplement which is capable of rapidly increasing the blood concentration of lactic acid and ammonia in the body after or after exercise such as STIMOL SOLUTION, It dissolves and increases the energy and helps the excretion of ammonia accumulated in the body through the urine. This is a product of this principle, such as football players who need muscle endurance, athletes who have a lot of exercise power training, or post-operative lethargy patients can not eat meals can also be a meal.

The present invention also relates to a pharmaceutical composition for oral administration of fruit juice of vitamin A to a newborn, toddler, and infant as a pharmaceutical preparation and / or a nutritional supplement, Milk, and mammalian milk substitutes. The juice of the vitamin Nut fruit of the present invention may also be administered in the form of its pharmaceutically active salts. Suitable pharmaceutically active salts include acid addition salts and alkali salts or alkaline earth metal salts. For example, sodium, potassium, lithium, magnesium or calcium salts may be obtained. The pharmaceutical compositions of the present invention are typically formulated for a desired dosage form, that is, tablets, capsules (solid, semi-solid or liquid), powders for constitution, suitable carriers selected for oral administration in the form of aerosol preparations, ≪ / RTI >

Another aspect of the invention relates to the use of the disclosed vitamin nuts juice as a nutritional supplement. Nutritional supplements are preferred for oral administration, but are not limited thereto. Nutritional supplements are used to supplement daily food. The "dietary components" of these products may also include vitamins, minerals, herbs or other plant components, amino acids, and substances such as enzymes, organ systems, glands, and metabolites. Nutritional supplements may be prepared, for example, in the form of tablets, capsules, soft gels, gel caps, liquids, or powders.

Another aspect of the present invention relates to a method for the treatment and / or prophylaxis of diabetes, hyperlipidemia, hyperlipidemia, hyperlipidemia, hyperlipidemia, And a method for preventing and / or treating a related disease such as liver disease.

The present invention may be better understood by the following examples, which are for the purpose of illustrating the invention and are not intended to limit the scope of protection defined by the appended claims.

Example  One: Vitamin tree  Manufacture of fruit juice

From November to December in the Inner Mongolia area, the harvested vitamin tree was purchased and its stem was removed to obtain fruit, which was then divided into crusts and pulp through a pulverizing process. Then, the separated pulp was squeezed using a squeezer, and the oil component of the juice produced after squeezing was left unremoved.

Subsequently, homogenization of the pulp was performed under a high pressure homogenization process at a pressure of 30 MPa to homogenize the pulp, and the liquid was filtered through a 120 mesh filter net. Thereafter, the filtered liquid was injected into the automatic branching machine and then bottled. The bottled juice was sealed with CAP, which is made of a polyethylene inner cap and a steel cap, and sterilized in a vacuum for about 10 minutes. After sterilization, it was rapidly cooled in an automatic cooling water tank.

Experimental Example  One: Vitamin tree  Identify ingredients of fruit juice

The components contained in 100 g of the vitamin nut fruit juice prepared in Example 1 were examined and the results are shown in Table 1 below.

Essential amino acid Isoleucine 21.24 mg Leucine 32.07 mg Lysine 31.38 mg Methionine 6.07 mg Phenylalanine 31.85 mg Threonine 23.22 mg Balin 26.71 mg
fatty acid Linolenic acid ( Omega 3 ) 18.17 mg Linoleic acid  ( Omega 6 ) 52.09 mg Palmitoleic acid ( Omega 7 ) 220.11 mg Oleic acid ( Omega 9 ) 118.15 mg Palmitic acid 239.64 mg
mineral Sodium (Na) 26.01 mg Copper (Cu) 0.09 mg Zinc (Zn) 0.26 mg Iron (Fe) 4.09 mg Magnesium (Mg) 10.00 mg Calcium (Ca) 32.21 mg
Other selenium 7.73 μg Soluble fiber 6.18 mg Saponin 259 mg Total flavonoid 191 mg
nutrient calorie 55.84 Kcal carbohydrate 11.51 g protein 1.01 g Fat 0.64 g Fiber 0.08 g
vitamin Beta-carotene 1.06 mg B1 0.44 mg B2 0.07 mg B6 0.71 mg C 146.03 mg D 2.83 μg E 2.27 mg Folic acid 38.56 μg Nei Asin 24.66 mgNE
amino acid Histidine 13.15 mg Glycine 24.20 mg Arginine 60.33 mg Alanine 12.59 mg Tyrosine 10.93 mg Serine 28.34 mg Glutamine 66.50 mg Asparagine 205.40 mg Proline 51.46 mg

That is, as shown in Table 1, the vitamin Nut fruit juice according to the present invention not only contains vitamins together with fatty acids, but also contains a variety of essential amino acids, minerals, and nutrients. That is, the fruit juice of the vitamin tree according to the present invention contains about 650 mg of fatty acid and about 170 mg of vitamin per 100 g, and it can be confirmed that a large amount of omega fatty acid and vitamin are contained together.

Experimental Example  2: Vitamin tree  Fruit juice Glucose , Cholesterol and Triglyceride  Reduction effect

The clinical trial was conducted with the vitamin Nut fruit juice prepared in Example 1 above. The composition used in clinical use was the fruit juice obtained from the vitamin 1 fruit juice prepared in Example 1, which was stored in a 50 ml bottle for about one year in a vacuum state in the shade to induce the enzyme action.

Experimental Example  2-1: Test method

This Pilot test was conducted to examine the effect of vitamin Nut fruit juice according to the present invention on the blood lipid concentration after feeding for 3 weeks. 300 ml of 100 ml x 3 times a day for 3 weeks were allowed to drink before, during, and after breakfast, lunch and dinner. All subjects were prohibited from using any dietary supplementation during the period of the experiment. All items were recorded separately when using other foods other than those provided by the dormitory.

1) Blood collection

Subjects participated in the experiment for more than 12 hours, and blood samples were collected from the venous blood of the upper arm on the day of the experiment. The collected blood was centrifuged and the serum was separated and stored at -80 ° C.

2) Blood lipid analysis

All serum samples stored at -80 ℃ were thawed at room temperature for analysis of blood lipids of subjects and were used for analysis using COBAS C111 equipment. Total cholesterol and triglyceride were measured optically using the principle of enzymatic colorimetric method. The analysis of glucose was based on the principle of enzymatic reference method with hexokinase, and the production rate of NADPH formed during the reaction was optically measured .

Experimental Example  2-2: Test results

The results of the test as described above are shown in FIG. 2 and Table 2 below.

One 2 3 4 5 P -value
Pre Post Pre Post Pre Post Pre Post Pre Post cholesterol 230.98 207.9 200.31 188.61 153.22 148.42 181.09 172.3 82.28 73.98 0.043 Glucose 85.97 63.76 87.73 73.21 87.25 73.57 86.26 73.98 89.27 70.97 0.043 Triglyceride 860.11 674.77 282.61 143.25 48.46 51.74 82.28 48.8 69.29 80.31 0.225

That is, as shown in the above Table 2, when the vitamin N fruit juice according to the present invention was consumed, glucose and cholesterol were decreased in all users, and the triglyceride was remarkably decreased in most users.

While the present invention has been particularly shown and described with reference to preferred embodiments thereof, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It will be apparent to those skilled in the art that changes may be made.

Claims (5)

A food composition for prevention of fatty liver comprising fruit juice of a vitamin tree (Hippophae rhamnoides L.)
The fruit juice includes fatty acids, vitamins, essential amino acids, minerals and amino acids,
Wherein the fatty acid comprises omega 3, omega 6, omega 7, omega 9 and palmitic acid,
Wherein said vitamin comprises beta carotene, B1, B2, B6, C, D, E, folic acid and niacin,
The essential amino acids include isoleucine, leucine, lysine, methionine, phenylalanine, threonine, and valine,
Wherein the mineral comprises sodium, copper, zinc, iron, magnesium and calcium,
Wherein the amino acid comprises histidine, glycine, arginine, alanine, tyrosine, serine, glutamine, asparagine and proline,
The fatty acid contained 650 mg per 100 g of the fruit juice,
The vitamin contains 170 mg per 100 g of the fruit juice,
The fruit juice of the vitamin tree,
The leaves and stems were removed from the vitamin tree to obtain fruit,
The above-mentioned fruit husk is removed to obtain a lysine flesh,
The fruit juice can be obtained by squeezing the above-
Wherein the juice comprises water by 70%. delete delete delete delete

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