KR101708039B1 - Optical Measuring Apparatus And Cuvette Holder therein - Google Patents
Optical Measuring Apparatus And Cuvette Holder therein Download PDFInfo
- Publication number
- KR101708039B1 KR101708039B1 KR1020150075377A KR20150075377A KR101708039B1 KR 101708039 B1 KR101708039 B1 KR 101708039B1 KR 1020150075377 A KR1020150075377 A KR 1020150075377A KR 20150075377 A KR20150075377 A KR 20150075377A KR 101708039 B1 KR101708039 B1 KR 101708039B1
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- South Korea
- Prior art keywords
- cuvette
- light
- window
- exit window
- sample
- Prior art date
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
- G01N2021/3122—Atomic absorption analysis using a broad source with a monochromator
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
- G01N2021/317—Special constructive features
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
An optical analyzing apparatus according to an aspect of the present invention includes a lamp, an excitation monochromator for extracting and exciting specific monochromatic light from the light generated from the lamp, an excitation monochromator for exciting the excited light from the sample, A light absorbing module for absorbing and analyzing the light irradiated to the sample and a cuvette on which the sample is to be placed, the light emitted from the sample is absorbed by at least one of the emission monochromator and the light absorbing module And a cuvette holder for selectively controlling the cuvette holder to advance.
Description
The present invention relates to an optical analyzer for analyzing an optical spectrum of a sample and a cuvette holder included therein, and more particularly, to a cuvette holder capable of selectively performing a light absorption analysis and a fluorescence analysis by selectively controlling a light path in a cuvette holder And an optical analysis apparatus having the same.
Fluorescence is a phenomenon in which a molecule absorbs light with a short wavelength of high energy and emits light with a wavelength longer than that. When a molecule absorbs light, it moves up to a higher energy excited state. When a molecule that absorbs light ascends to an excited state, it becomes unstable and emits energy in the form of heat or emits light of a different wavelength and falls into its original state.
A fluorescence spectrophotometer, known as a fluorescence spectrometer, measures the fluorescence emitted from a material to determine how much fluorescence is emitted at which wavelength.
Specifically, a fluorescence spectrophotometer excites light generated in a lamp by excitation at an excitation monochromator and irradiates the sample. The light emitted from the sample is passed through an emission monochromator and then detected by a fluorescence detector Analysis.
However, in the conventional optical analysis apparatus, only the fluorescence analysis can be performed in the case of the fluorescence analysis apparatus, and the UV-Vis absorption spectroscopic analysis can not be performed. On the contrary, the analysis apparatus performing the UV-Vis absorption spectroscopy analysis performs fluorescence spectroscopy Therefore, there is a problem in that, in the case of a sample suitable for each analysis, it is necessary to provide separate equipments for analysis.
Embodiments of the present invention provide an optical analysis apparatus capable of performing absorption analysis in a fluorescence analysis apparatus, and a cuvette holder used therein, in order to overcome the problems of the prior art described above.
An optical analyzing apparatus according to an aspect of the present invention includes a lamp for generating light, an excitation monochromator for extracting and exciting specific monochromatic light from the light generated from the lamp, an excitation monochromator for exciting the excited light to a sample, A light absorbing module for absorbing and analyzing the light received by the sample and a cuvette on which the sample is placed, the light emitted from the sample is absorbed by the emission monochromator and the light absorbing And a cuvette holder for controlling the cuvette holder to select at least one of the modules.
The cuvette holder includes a cuvette insertion hole into which the cuvette is inserted, an incidence window opened to the cuvette inserted into the cuvette insertion hole, a first exit window formed to be opened by a path perpendicular to the incidence window, A first screen door capable of opening and closing an exit window, a second exit window formed on a path aligned with the incident window, and a second screen door capable of opening and closing the second exit window.
The cuvette holder includes a first insertion channel in which a first screen door for opening and closing the first exit window is inserted and slid, and a second insertion port in which a second screen door for opening and closing the second exit window is inserted and slid Channel.
Further, the incident window, the first exit window, and the second exit window are configured to correspond to the side surface of the cuvette when the cuvette is mounted on the cuvette insertion hole.
Meanwhile, the optical analyzing apparatus according to an embodiment of the present invention further includes at least one reflector for controlling the light emitted from the excitation monochromator to proceed toward the cuvette holder.
In addition, the optical analyzing apparatus according to an embodiment of the present invention includes a beam splitter on the optical path between the reflecting unit and the cuvette holder.
In addition, an image sensor for alignment is provided on the lower side of the beam splitter to check whether the optical elements are aligned as intended.
According to another aspect of the present invention, there is provided a cuvette holder for receiving a cuvette on which a sample is mounted, irradiating the excited light to the sample, and then selecting the light emitted from the sample as at least one of an absorption measurement mode and a fluorescence measurement mode The cuvette holder includes a cuvette insertion hole into which the cuvette is inserted, an incidence window opened to the cuvette inserted into the cuvette insertion hole, and a cuvette holder which is opened and formed by a path perpendicular to the incidence window, A second exit window formed on the path aligned with the incidence window, and a second exit window formed on the path aligned with the incidence window, the second exit window being openable and closable, the first exit window having a first exit window, Screen doors.
The following effects can be realized by the optical analyzing apparatus according to the embodiment of the present invention.
First, since both the fluorescence analysis and the absorption analysis can be performed in one optical analyzing apparatus, it becomes possible to analyze the absorption spectrum as a fluorescence measuring apparatus, thereby increasing the usability of the apparatus.
Second, the direction of light introduced into the cuvette holder in the fluorescence mode and the direction of the emission monochromator for fluorescence analysis are arranged perpendicular to each other, thereby preventing the intensity of the light from being lowered.
Third, the direction of the light introduced into the cuvette holder in the fluorescence mode and the direction toward the emission monochromator for fluorescence analysis are arranged perpendicular to each other, thereby increasing the space utilization and reducing the volume of the device as a whole.
Meanwhile, according to the cuvette holder according to the embodiment of the present invention, the following effects can be realized.
First, since the cuvette holder of the embodiment of the present invention can easily perform the switching between the fluorescence mode and the light absorption mode, by using the cuvette holder, the optical analysis apparatus can perform two kinds of optical analysis.
Secondly, the cuvette holder of the embodiment of the present invention has an advantage that the mode change can be easily performed by simply raising or lowering the screen door to convert between the fluorescence mode and the light absorption mode.
FIG. 1 is a perspective view illustrating an optical absorption mode of an optical analyzing apparatus according to an embodiment of the present invention. Referring to FIG.
2 is a perspective view of a cuvette holder and a cuvette used in the optical analysis apparatus of the present invention.
FIG. 3 is a partially enlarged view showing the state of the screen door of the cuvette holder in the light absorption mode of FIG. 1;
4 is a perspective view of a fluorescence mode state of the optical analyzing apparatus according to an embodiment of the present invention.
FIG. 5 is a partially enlarged view showing the state of the screen door of the cuvette holder in the fluorescent mode state of FIG. 4;
BRIEF DESCRIPTION OF THE DRAWINGS The present invention will become more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. The present invention may, however, be embodied in many different forms and should not be construed as being limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Is provided to fully convey the scope of the invention to those skilled in the art, and the invention is only defined by the scope of the claims. It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
In the present specification, the singular form includes plural forms unless otherwise specified in the specification. It is noted that the terms "comprises" and / or "comprising" used in the specification are intended to be inclusive in a manner similar to the components, steps, operations, and / Or additions. The terms first, second, etc. may be used to describe various elements, but the elements should not be limited by terms. Terms are used only for the purpose of distinguishing one component from another.
2 is a perspective view of a cuvette holder and a cuvette used in the optical analysis apparatus of the present invention, and Fig. 3 is a cross-sectional view of the cuvette holder and the cuvette used in the optical analysis apparatus of the present invention. Fig. 5 is a partially enlarged view showing the state of the screen door of the cuvette holder in the light absorption mode. Fig.
An
The
The
1, the light generated by the
The light irradiated from the
The light reflected by the second reflecting
Light entering the light
1 to 3 show a case where the
The
1 to 3, the light emitted from the sample by the excited light irradiated on the
In this case, the
Hereinafter, the specific structure of the cuvette holder will be described. The principle of selectively guiding the light emitted from the sample to the cuvette holder by the
2 shows an enlarged view of a specific configuration of the
The
The
The
The
The
The
The
The
2, the
FIG. 3 shows a path through which the light travels by the
2 and 3, the
Accordingly, the excited light introduced into the
Meanwhile, although not clearly shown in the drawing, in the mode in which the first screen door or the second screen door is raised to the upper side, the
Similar fitting or complementary fastening structures may be applied between the
FIG. 4 is a perspective view of a fluorescence mode of an optical analysis apparatus according to an embodiment of the present invention, and FIG. 5 is a partially enlarged view showing a state of a screen door of a cuvette holder in the fluorescence mode of FIG.
4 and 5 are different from those of FIGS. 1 to 3 in the setting of the screen door of the cuvette holder, and accordingly, the path of the light emitted from the
4 and 5, the light generated by the
The light irradiated from the
The light reflected by the second reflecting
Light entering the light path control
4 and 5 show a case in which the
In FIGS. 4 and 5, the progress path of light controlled in the
Therefore, the light emitted from the sample in the
FIG. 5 is an enlarged view of a specific configuration of the
5, since the
Therefore, the excited light introduced into the
Although the present invention has been described in connection with the above-mentioned preferred embodiments, it is possible to make various modifications and variations without departing from the spirit and scope of the invention. Accordingly, it is intended that the appended claims cover all such modifications and variations as fall within the true spirit of the invention.
100: Optical analysis device
102: Base portion
110: Here, the monochromator
120: light path control unit
122: Cuvette holder
122a: first screen door 122aa: first screen door handle
122ab: first screen door screen part 122ac: first screen door insertion channel
122b: second screen door 122ba: second screen door handle
122bb: second screen door screen part 122bc: second screen door insertion channel
122c:
122e:
122g: 2nd outgoing window
127: Cuvette insert ball
130: lamp
140: Emissive monochromator
Claims (8)
An excitation monochromator for extracting and exciting specific monochromatic light from the light generated from the lamp;
An emission monochromator for extracting a predetermined monochromatic light from the light emitted from the sample after irradiating the excitation light to the sample;
A light absorbing module for absorbing and analyzing the light irradiated to the sample; And
And a cuvette holder configured to receive the cuvette on which the sample is mounted and to control the light emitted from the sample to be selected as at least one of the emission monochromator and the light absorption module,
The cuvette holder
A cuvette insertion hole into which the cuvette is inserted;
An incidence window opened to the cuvette inserted in the cuvette insertion hole;
A first exit window formed to be opened in a path perpendicular to the incident window;
A first screen door capable of opening and closing said first exit window;
A second exit window formed on the path aligned with the incident window;
A second screen door capable of opening and closing the second exit window;
A first insertion channel through which a first screen door for opening and closing the first exit window is inserted and slid; And
And a second inserting channel for inserting and sliding the second screen door for opening and closing the second exit window.
Wherein the incidence window, the first exit window, and the second exit window correspond to a side surface of the cuvette when the cuvette is mounted on the cuvette insertion hole.
Further comprising at least one reflector for controlling the light emitted from the excitation monochromator to proceed toward the cuvette holder.
The cuvette holder
A cuvette insertion hole into which the cuvette is inserted;
An incidence window opened to the cuvette inserted in the cuvette insertion hole;
A first exit window formed to be opened in a path perpendicular to the incident window;
A first screen door capable of opening and closing said first exit window;
A second exit window formed on the path aligned with the incident window;
A second screen door capable of opening and closing the second exit window;
A first insertion channel through which a first screen door for opening and closing the first exit window is inserted and slid; And
And a second insertion channel in which a second screen door for opening and closing the second exit window is inserted and slid.
Wherein the incidence window, the first exit window, and the second exit window correspond to a side surface of the cuvette when the cuvette is mounted on the cuvette insertion hole.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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KR1020150075377A KR101708039B1 (en) | 2015-05-28 | 2015-05-28 | Optical Measuring Apparatus And Cuvette Holder therein |
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KR1020150075377A KR101708039B1 (en) | 2015-05-28 | 2015-05-28 | Optical Measuring Apparatus And Cuvette Holder therein |
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KR20160139819A KR20160139819A (en) | 2016-12-07 |
KR101708039B1 true KR101708039B1 (en) | 2017-02-17 |
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CN110487764B (en) * | 2019-08-30 | 2024-06-21 | 天津陆海石油设备系统工程有限责任公司 | Manual and automatic sample feeding mode switching device of quantitative fluorescence analyzer |
CN111948161B (en) * | 2020-09-11 | 2024-06-11 | 上海元析仪器有限公司 | Multipurpose colorimetric combination rack for spectrophotometer |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005513497A (en) * | 2002-01-03 | 2005-05-12 | カール ツァイス イエナ ゲゼルシャフト ミット ベシュレンクテル ハフツング | Method and / or apparatus for identification of fluorescent, luminescent and / or light-absorbing substances on and / or in a sample carrier |
JP4143512B2 (en) * | 2003-10-07 | 2008-09-03 | 株式会社堀場製作所 | Spectrophotometer |
JP2014524582A (en) | 2011-08-23 | 2014-09-22 | バイオクロム リミテッド | Cuvette |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100489405B1 (en) * | 2004-09-24 | 2005-05-12 | 큐딕스 (주) | DLS-type real time particle size analyzer |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005513497A (en) * | 2002-01-03 | 2005-05-12 | カール ツァイス イエナ ゲゼルシャフト ミット ベシュレンクテル ハフツング | Method and / or apparatus for identification of fluorescent, luminescent and / or light-absorbing substances on and / or in a sample carrier |
JP4143512B2 (en) * | 2003-10-07 | 2008-09-03 | 株式会社堀場製作所 | Spectrophotometer |
JP2014524582A (en) | 2011-08-23 | 2014-09-22 | バイオクロム リミテッド | Cuvette |
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