KR101660382B1

KR101660382B1 - The manufacturing method of safflower drinking using powder fermentation for safflower seeds

Info

Publication number: KR101660382B1
Application number: KR1020150185580A
Authority: KR
Inventors: 송형성
Original assignee: 산청군; 송형성
Priority date: 2015-12-24
Filing date: 2015-12-24
Publication date: 2016-09-27

Abstract

The present invention relates to a method for producing a safflower beverage using fermented safflower seed powder, and more particularly, to a safflower beverage making method using a fermented safflower seed powder capable of completely removing oil components of safflower seed.
The method of manufacturing a safflower beverage using the fermented safflower seed powder according to an embodiment of the present invention comprises: a first step of roasting safflower seeds washed at 160 DEG C for 10 minutes to firstly milk; A second step of feeding the safflower seeds in the distilled water, boiling them, and heating the same to make the second milking; A third step of naturally fermenting the second milking safflower seed at 30 to 35 DEG C for 48 hours to ferment the microorganism; The fermenting microorganism is fermented by adding a fermenting microorganism to the natural fermented safflower seed, wherein the fermenting microorganism is selected from the group consisting of Bacillus sp., Bacillus macerans, Bacillus circulas, Bacillus coagulans ) Strain of the present invention; A fifth step of drying the microorganism-fermented safflower seed at 40 to 45 DEG C for 5 to 10 hours; A sixth step of pulverizing the dried safflower seed into 20 to 50 mesh; Mixing the 100 parts by weight of purified water with 1 part by weight of the pulped safflower seed, mixing the same at a low temperature of 40 to 60 DEG C for 10 to 20 minutes, and then filtering to prepare an extract of safflower seed; Mixing the sugar and the acidifier into the safflower seed extract, cooling the safflower seed extract to 5 to 8 ° C, and then adding carbonic acid gas at a pressure of 0.3 to 1.0 kg / cm 2 to produce a safflower seed carbonated beverage; And the eighth step of sterilizing the safflower seed extract and the safflower seed carbonated drink at 70 to 75 DEG C for 5 to 10 minutes.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a safflower beverage comprising safflower seed powder,

The present invention relates to a method for preparing a safflower beverage by using fermented safflower seed powder, and more particularly, to a method for producing a safflower beverage by completely removing the oil component of safflower seed, The present invention also relates to a method for producing safflower beverage using the safflower safflower seed powder having enhanced functionality and flavor.

Recently, studies supporting the weakness of safflower seed have been carried out at the Food Nutrition Society, the Korean Periodontal Society, the Korean Society of Orthopedic Surgery, the Korean Society of Inflammation, the Korean Fracture Society, and the Korean Society of Veterinary Pathology.

In addition, development of new medicines using safflower seed is underway in the pharmacy field, and development of health functional foods using safflower seed is underway in the food and nutrition science field.

Safflower seeds are white in color and have a white appearance and are ripened in July and August. They are widely used for weak osteoporosis and weak bones. Especially, it increases the bone density of osteoporosis and fracture of elderly people after menopause. It is well known to make it easy and fast.

It is known that safflower seed can be used as powder, ring, and edible oil, and it has a good effect on bone disease in the private sector, and consumption of the user is gradually increasing. Recently, there have been various studies for developing products having improved functionality and taste by using safflower seeds or roasted seeds in beverage products, oriental drinks, confectionery, and bakery industries.

However, conventionally, there have been various problems in manufacturing and selling safflower seed as a beverage. The fact that the oil contained in safflower seeds is easy to rancor and that the residual oil inhibits the inherent taste of the product.

Meanwhile, Korean Patent Registration No. 10-1331427 describes a method for producing various beverages using safflower seeds germinated. When the beverage is prepared by fermenting safflower seed after roasting, extraction, filtration, etc., the present invention not only satisfies consumers' preferences but also includes a high content of nutrients and improves shelf life and shelf life of the product It is possible to provide a food formulation that can be helpful for health promotion as well as palatability.

As disclosed in Korean Patent No. 10-1331427, when safflower seeds are germinated to prepare beverages, the content of tocopherol and the amino acid content are increased to help improve health. However, the actual germinated safflower seeds can be produced as beverages There is a problem that the taste is deteriorated due to the residual oil or the quality of the fragrance or color is lowered.

In order to solve the above-described problems, the present invention provides a method for producing a microorganism by converting a low-molecular-weight useful ingredient into a microbial fermentation process capable of easily absorbing the human body and enhancing the functionality and flavor thereof by producing secondary metabolites of an edible microorganism, And to provide a high-quality safflower beverage manufacturing method.

Korean Patent No. 10-1331427

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made in order to solve the above problems, and it is an object of the present invention to provide a method of manufacturing safflower beverages which can completely remove oil remaining in safflower seeds through roasting, microbial fermentation, have.

The method of manufacturing a safflower beverage using the fermented safflower seed powder according to an embodiment of the present invention comprises: a first step of roasting safflower seeds washed at 160 DEG C for 10 minutes to firstly milk; A second step of feeding the safflower seeds in the distilled water, boiling them, and heating the same to make the second milking; A third step of naturally fermenting the second milking safflower seed at 30 to 35 DEG C for 48 hours to ferment the microorganism; The fermenting microorganism is added to the natural fermented safflower seed, wherein the fermenting microorganism is selected from the group consisting of Bacillus sp., Bacillus macerans, Bacillus circulas, Bacillus coagulans A fourth step of microbial fermentation with any one selected; A fifth step of drying the microorganism-fermented safflower seed at 40 to 45 DEG C for 5 to 10 hours; A sixth step of pulverizing the dried safflower seed into 20 to 50 mesh; A seventh step of mixing 100 to 150 parts by weight of purified water with 1 part by weight of the pulped safflower seed, extracting it at a low temperature of 40 to 60 DEG C for 10 to 20 minutes, and then filtering to obtain an extract of safflower seed; And sterilizing the safflower seed extract at 70 to 75 DEG C for 5 to 10 minutes.

The safflower beverage manufacturing method using the fermented safflower seed powder according to the present invention has the effect of completely removing the oil remaining in the safflower seed by fermenting the first and second milking safflower seeds.

In addition, there is an effect that safflower beverages having improved taste and improved functionality and palatability can be produced as compared with beverages made with roasted safflower seeds by a general method.

In addition, a person with a weak digestion period can induce diarrhea upon excessive consumption of safflower seed, and it is effective to produce safflower beverage having no side effects by producing safflower beverage using fermented safflower seed.

In addition, natural pigments of safflower seeds fermented as compared with other beverages that add color by adding an edible pigment have the effect of further increasing the taste and flavor of the consumer.

In addition, the general ingredients, amino acids, minerals, free sugars, vitamins and fatty acids are more increased than beverages made with roasted safflower seeds in general manner, so that safflower beverages manufactured as health beverages can be produced.

1 is a flowchart showing a method of manufacturing a safflower beverage using a fermented safflower seed powder according to an embodiment of the present invention
Fig. 2 is a graph comparing the general components of common safflower seeds and fermented safflower seeds
3 is a graph comparing the amino acid compositions of common safflower seeds and fermented safflower seeds
4 is a graph comparing the mineral composition of common safflower seeds and fermented safflower seeds
Figure 5 is a graph comparing the composition of free sugar of common safflower seeds and fermented safflower seeds
Fig. 6 is a graph comparing the vitamin contents of common safflower seeds and fermented safflower seeds
7 is a graph comparing the fatty acid contents of common safflower seeds and fermented safflower seeds

The present invention relates to a method for producing a safflower beverage using fermented safflower seed powder, and more particularly, to a safflower beverage making method using a fermented safflower seed powder capable of completely removing the oil component of safflower seed.

The above and other objects, features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention and the manner of achieving them will become apparent by reference to an embodiment which will be described in detail below with reference to the accompanying drawings.

Hereinafter, a method for producing safflower beverage using the above-described fermented safflower seed powder will be described in detail with reference to the drawings.

1 is a flow chart showing a flow of a method for producing safflower beverage using the fermented safflower seed powder according to the present invention.

First, in the first step (S100), safflower seeds without peel are roasted at 160 DEG C for 10 minutes to be firstly milked.

The safflower seeds are roasted at 160 DEG C for 10 minutes to heat the surface of the safflower seed so that the sour safflower beverage can be prevented from spoiling and the circulation period can be increased by evaporating the safflower seed oil.

Conventionally, it was most common to roast at 70 to 80 ° C for 30 minutes at the first milking. However, when roasting at 70 to 80 ° C for 30 minutes, the fat can not be sufficiently removed, and when roasted at 160 ° C for 10 minutes, But the flavor preference of the prepared beverage is lowered.

However, in the present invention, the first step (S100) is roasted at 160 DEG C for 10 minutes, and then the second-stage milking step (S200) is performed to remove the bitterness or bitterness. Therefore, it is possible to increase the distribution period by preventing the effect of increasing taste preference and rancidity.

Further, through the following Example 2, the most effective roasting temperature condition at the first milking can be known.

Next, in the second step (S200), the safflower seeded with the first milk is put into distilled water and boiled to heat the second milk. Specifically, it is preferable to mix 10 parts by weight of distilled water with 1 part by weight of safflower seed obtained by primary milking, and milking at 50 to 60 캜.

The safflower seeds are added to the distilled water and heated so that the oil remaining in the safflower seeds is discharged to the outside and the bitter taste or bitterness is removed.

When 1 part by weight of the first milking safflower is mixed with less than 10 parts by weight of distilled water, the first milking safflower seeds may not be completely immersed so that the oil remaining in the safflower seeds may not be completely discharged, , The second milking time is too long, so it is preferable to perform the second milking under the above conditions.

In addition, it is preferable to perform the second milking of the first milking safflower at 50 to 60 DEG C, and since the temperature is too low at the second milking at less than 50 DEG C, the second milking time takes too long, In case of second milking in excess, the residual oil in the safflower seeds is insignificantly discharged, which accelerates the rancidity of the safflower beverage produced by the residual oil, and the bitter taste and the bitter taste of the safflower beverage are lowered, Most preferred.

Next, in a third step (S300), microbes are naturally fermented so as to naturally propagate in the second milking safflower seed. Specifically, it is preferable to naturally ferment at 30 to 35 캜 for 48 hours so as to maintain the relative humidity at 60%.

The safflower seed is largely removed by passing through the first milking and the second milking. The safflower seed is fermented through the natural fermentation in the third step (S300) and the microbial fermentation in the fourth step (S400) The flavor of the safflower beverage produced by the method of the present invention is added.

In the third fermentation step S300, it is most preferable that the relative humidity is 60%. If the relative humidity is less than 60%, natural fermentation in which the microorganisms naturally propagate may be insignificant, and the relative humidity may be 60% It is preferable to spontaneously grow the microorganism under the above conditions since the naturally grown microorganism may be altered or the microorganism harmful to health may naturally multiply.

If the temperature is lower than 30 ° C, the relative humidity is less likely to be 60%, resulting in microbial fermentation which is detrimental to microorganisms or harmful to health. When the fermentation temperature exceeds 35 ° C , It is preferable to naturally ferment under the above conditions since the sourdough can be produced in the prepared safflower beverage.

If the fermentation time is less than 48 hours, the relative humidity is less likely to be 60%, so that the fermentation of the microorganisms may be deteriorated or harmful to health. If the fermentation time exceeds 48 hours It is preferable to perform the natural fermentation under the above conditions since the produced safflower beverages may grow in the mouth.

In addition to the beneficial bacteria in the process of natural fermentation of the microorganisms, there is no possibility of the propagation or pollution of the germs. However, the presence of any germs was not detected in several tests, It is thought that the growth of germs is inhibited or interfered by the action of enzymes or antagonistic substances secreted by beneficial bacteria or fungi that predominate by proliferation first, even if the germs sometimes infiltrate by the antibacterial action.

Next, in a fourth step (S400), fermentation microorganisms are added to the safflower seed which is naturally fermented by the growth of the microorganisms, thereby carrying out microbial fermentation.

Specifically, the fermenting microorganism is preferably any one selected from the group consisting of Bacillus sp., Bacillus macerans, Bacillus circulas, and Bacillus coagulans.

The above-mentioned Bacillus sp. Is an aerobic or tuberous anaerobic bacterium widely distributed in nature, and is involved in denitrification, fermentation source, oxidation of manganese, and the like.

Bacillus macerans is a bacterium having an enzyme that synthesizes cyclodextrin. When it is fed starch or saccharide, it is decomposed and absorbed into monosaccharide glucose. In this case, when Bacillus macerans, a cyclodextrin synthase, is supplied, Approximately half of the amount absorbed by degradation is converted to oligosaccharides called cyclodextrins, which are highly water-absorbing, and excreted intact. Therefore, it has been studied as a preventive medicine for diabetes, and constipation can be prevented.

The Bacillus circulas produce the antipyretic polypeptides.

The Bacillus coagulans is a beneficial bacterium having a similar action to probiotics or Lactobacillus. It is generally used for the treatment of diarrhea and infectious diseases. It is also useful for the treatment of indigestion, irritable bowel syndrome, and inflammatory bowel disease.

In the fourth step (S400), microbial fermentation may induce diarrhea, thereby reducing the side effects of safflower seed causing digestion failure, and increasing the taste and flavor of the safflower beverage. Therefore, desirable.

Next, in a fifth step S500, the fermented safflower seeds are dried at 40 to 45 DEG C for 5 to 10 hours so that the moisture content is 5 to 20 w / w%.

Preferably, the drying step is performed such that the moisture content of the fermented safflower seed is 5 to 20 w / w%, and when the water content is less than 5 w / w% or more than 20 w / w% It may be difficult to pulverize in the sixth step (S600). Therefore, drying under the above conditions is preferable.

In addition, drying at a temperature lower than 40 캜 has a disadvantage in that the drying time is too long, and when the drying temperature exceeds 45 캜, the moisture content exceeds 20 w / w%.

In addition, when drying is performed for less than 5 hours or for more than 10 hours, the moisture content is exceeded, so it is preferable to dry under the above-mentioned time conditions.

Next, in a sixth step (S600), the dried safflower seed is crushed. Specifically, the safflower seed dried in the sixth step (S600) is preferably pulverized to a size of 20 to 50 mesh since the safflower seeds are contained in the safflower seed.

Since the shell portion of the dried safflower seeds contains a large amount of essential inorganic components such as calcium, potassium, magnesium, iron and zinc, it is preferable to crush the safflower seeds together.

Next, in a seventh step (S700), 100 parts by weight of purified water is mixed with 1 part by weight of the pulped safflower seed, extracted at a low temperature of 30 to 40 DEG C for 10 to 20 minutes, and filtered to prepare an extract of safflower seed.

When 1 part by weight of pulverized safflower seeds is mixed with less than 100 parts by weight of purified water, flavor of the safflower beverage can be improved, and when it exceeds 150 parts by weight, extraction takes a long time at low temperature. Therefore, .

If the temperature is lower than 30 ° C, the extraction may be difficult. If the temperature is higher than 40 ° C, the temperature may be too high, so that the carbonic acid gas in step 7-1 (S701) It is preferable to perform extraction under the above conditions since the charging process may not proceed.

It is preferable that the extraction time is 10 to 20 minutes. If the extraction time is less than 10 minutes, the extraction may not be performed sufficiently. If the extraction time exceeds 20 minutes, the taste of the safflower beverage may be deteriorated. It is preferable to extract it under the conditions.

Next, in step 7-1 (S701), carbonated gas is filled into the safflower seed extract to produce a safflower seed carbonated beverage. Specifically, it is preferable to mix the saccharide and the acidifier in the safflower seed extract, cool it to 5 to 8 ° C, and then fill it with carbonic acid gas at a pressure of 0.3 to 1.0 kg / cm 2.

Specifically, the sugar is preferably selected from among fructose, sugar, oligosaccharide, and honey.

It is preferable that the acidulant is any one selected from citric acid, malic acid and ascorbic acid.

The mixing ratio of sugar and acidifier to the safflower seed extract is preferably from 7 to 15 parts by weight per 100 parts by weight of the safflower seed extract and from 0.01 to 0.1 part by weight of the acidulant.

In order to improve the palatability of the safflower beverage of the present invention, the sugar and the acidulant are added to the safflower seed extract to adjust the saccharide acid ratio. The sugar and the acidulant added to the safflower seed extract are not particularly limited, but the sugar and the acidulant described above can increase the degree of preference, and mixing in the mixing ratio can increase the flavor of the safflower beverage.

In addition, the sugar and the acidifier are mixed and stirred in the safflower seed extract, and then cooled to about 5 to 8 캜 so as to facilitate carbon dioxide gas injection, and the temperature is kept constant.

The safflower seed extract is filled with carbonic acid gas at a pressure of 0.3 to 1.0 kg / cm 2 to prepare safflower beverage. If the pressure of the carbon dioxide gas is less than 0.3 kg / cm 2, the refreshing feeling of the sourdough beverage falls. If the pressure is 1.0 kg / cm 2 or more, the bitterness inherent in the safflower seed is strong. Most preferred.

The step 7-1 (S701) may be selectively performed according to the taste of the consumer.

Next, in an eighth step (S800), the safflower seed extract or safflower's carbonated beverage is sterilized at 70 to 75 DEG C for 5 to 10 minutes. Specifically, the safflower seed extract or the safflower seed carbonated beverage is filled into a can or a bottle using a can filling machine or a bottle filling machine, and the beverage is sealed and sterilized at 70 to 75 ° C for 5 to 10 minutes.

Hereinafter, the experiment contents prepared by using the fermented safflower seeds according to the present invention will be described in detail.

The components of the fermented safflower seeds used according to the present invention were analyzed as follows and the results are shown in Tables 1 to 6. In each table, general components, amino acid content, mineral content, free sugar composition, tocopherol content, vitamin content and fatty acid analysis of the common safflower seeds and fermented safflower seeds used in the present invention were compared and analyzed.

The powder samples used for the analysis of this component were stored at 4 ℃ after filling with nitrogen gas and used as experimental materials.

A. Comparison of general ingredients

Table 1 and FIG. 2 below show the general composition of common safflower seeds and fermented safflower seeds.

Moisture, crude protein, crude fat, crude ash and crude fiber were measured and quantified according to the AOAC method. That is, the moisture content was measured by the 105 ° C atmospheric pressure heating drying method, the crude fat was measured by Soxlet method, and the ash content was measured by dry painting method. The crude protein was quantified by Semimicro-Kjeldahl method using a Kelltec Auto Analyzer (Tecator) and the AOAC method (Hemmeberg-Stohmann) using a Fibertec System M-1020 (Tecator) Was calculated by subtracting the amount of the above-mentioned components.

Sample crude fiber
(Crude fiber) crude fat
(Crude fat) crude ash
(Minutes) crude protein
(Crude protein) N-free extract Safflower 38.54 + - 0.48 18.53 + 0.03 2.86 ± 0.03 16.61 + - 0.11 23.46 Fermented safflower seed 42.73 + - 0.38 16.60 ± 0.20 3.03 ± 0.01 18.32 + - 0.38 19.32

From Table 1 and FIG. 2, it can be seen that crude fiber, crude protein and crude protein were increased except crude fat and N-free extract of fermented safflower seed.

N. Comparison of amino acid composition

Table 2 and FIG. 3 below show the amino acid compositions of common safflower seeds and fermented safflower seeds in comparison.

The amino acid composition analysis was carried out according to the method of Heinrikson and Meredith. About 1 g of safflower seed powder was precisely weighed and placed in a muffle. After adding 15 ml of 6N HCl, the solution was hydrolyzed in a 150 ° C drying oven for 1 hour. The lyophilized solution was filtered through a Whatman No 2 filter paper after washing with a 50 ml volumetric flask . 2 ml of the above solution was diluted to 25 ml and then filtered through a 0.45 쨉 m membrane filter (Millipore Co., USA), and the amino acid composition was analyzed using AccQFluor ™ reagent kit (Waters, USA) according to the AccQTag method. That is, take 10 μl of the filtered free amino acid sample, carefully place it in the sample tube, add 70 μl of the first solution (AccQFluor Reagent Kit) of the AccQFluor Reagent Kit, mix with vortex mixer, aminoquinolyo-N-hydroxy succinimidyl carbamate (AQC)). The mixture is allowed to stand at room temperature for 1 minute, then derivatized at 55 ° C for 10 minutes, and then the amino acid is measured by HPLC. The amino acid standard used for the analysis was amino acid standard H (Pierce, USA) and the column was a Nova-pak column (Waters, USA).

amino acid Safflower Fermented safflower seed Asp
(Aspartic acid) 1516.3 1521.0 Ser
(Serine) 435.7 467.0 Glu
(Glutamic acid) 2808.1 2908.6 Gly
(Glycine) 789.9 795.7 His
(Histidine) 367.0 362.2 Thr
(Threonine) 369.2 387.9 Arg
(Arginine) 1337.7 1328.1 Ala
(Alanine) 537.8 537.9 Pro
(Proline) 553.8 556.1 Cys
(Cysteine) - - Tyr
(Threonine) 239.4 229.6 Val
(Balin) 699.4 683.2 Met
(Methionine) 38.2 87.8 Lys
(Lee Sin) 414.5 429.9 Isoleu
(Isoleucine) 481.5 473.5 Leu
(Lucin) 781.5 787.7 Phe
(Phenylalanine) 540.0 538.7 all 11910.1 12094.9

As shown in Table 2 and FIG. 3, it can be seen that the total amino acid content increased from 11910.1 to 12094.9, and the content of glutamic acid (Glu) increased in a considerable proportion.

C. Mineral composition comparison

Table 3 and FIG. 4 show the mineral composition of common safflower seeds and fermented safflower seeds.

The content of the nine kinds of the minerals of the safflower seed powder was quantified by a high frequency plasma method (Jobin Yvon Co., France) according to the AOAC method. After adding 10 g of deionized water to the ash obtained by dry-blending the sample with 4 g of the sample in a crucible at 500 ° C, 3 to 4 ml of HNO ₃ solution (HNO ₃ : H ₂ O = 1: 1) Evaporate and dry in the reactor. The mixture was further stirred at 500 ° C. for 1 hour and completely dissolved in 10 ml of HCl solution (HCl: H ₂ O = 1: 1). The mixture was adjusted to 50 ml and quantitatively analyzed by ICP atomic emission spectrophotometer.

Mineral composition Safflower Fermented safflower seed Na
(Sodium) 49.48 37.44 Al
(aluminum) 2.90 1.73 Cu
(Copper) 1.01 1.46 Ca
(calcium) 439.99 641.19 Fe
(iron) 5.38 7.78 Zn
(zinc) 4.12 6.71 K
(potassium) 729.52 882.02 Mg
(magnesium) 530.55 578.16 P
(sign) 535.05 538.06 all 2298.00 2694.55

As shown in Table 3 and FIG. 4, it can be seen that the total inorganic content increased from 2298.00 to 2694.55, and the content of sodium (Na) decreased and the content of calcium (Ca) and potassium (K) .

D. Comparison of free sugar composition

Table 4 and FIG. 5 show the free sugar composition of common safflower seeds and fermented safflower seeds.

For free sugar analysis, 10 g of the sample, which was degreased with diethyl ester according to the method of Wilson and Work, was added to 100 mL of 70% ethanol and reflux-cooled for 2 hours at 80 ° C in a water bath. The extract was filtered with Watman No. 1, and the filtrate was concentrated under reduced pressure at 40 ° C or less and diluted to 10 mL with distilled water. The solution was passed through an activated charcoal column to remove color, filtered, and passed through Sepak C ₁₈ (Waters Co.). The eluate was passed through a 0.45 μm membrane filter and analyzed by HPLC (high performance liquid chromatography). HPLC was performed using Jasco PU-980 (Jasco Co.).

Sample Fructose
(fruit sugar) Glucose
(glucose) Sucrose
(Sugar) Raffinose
(Raffinose) total Safflower 1.82 + - 0.67 65.11 + - 4.61 528.69 + - 0.74 625.29 + - 6.47 1,220.92 Fermented safflower seed 0.66 + 0.03 16.09 + - 0.66 649.89 + - 4.37 991.66 + - 8.21 1,658,312

From Table 4 and FIG. 5, it can be seen that the total inorganic content increased from 1,220.92 to 1,658,312, and the content of fructose and glucose was decreased, and the content of sugar (Sucrose) and Raffinose It can be seen that the content has increased at a considerable rate.

ㅁ. Vitamin content comparison

Table 5 and FIG. 6 show the vitamin contents of common safflower seeds and fermented safflower seeds.

The vitamin C content was determined by measuring oxidized and reduced ascorbic acid according to the method of Ikegaya et al.

To the oxidized form, 5 g of the safflower powder was added with 50 mL of 2% metaphosphoric acid solution, extracted at room temperature for 30 minutes, filtered, and used. The filtrate was filtered through a 0.45 μm membrane filter (Milex-LCR 13MM, PIFE, Millipore Co, Bedford, MS, USA) and analyzed by HPLC.

The reduced form was prepared by mixing 1 mL of dithiothreitol solution and 1 mL of neutralizing agent in 5 mL of the above filtrate, leaving it for 15 minutes, filtering it with a 0.45 μm syringe filter, and analyzing it by HPLC. HPLC was performed using Jasco PU-980 (Jasco Co.) and amino acid standard H (Pierce, USA) was used as the standard amino acid.

Sample alpha -tocopherol ? -tocopherol α-tocopherol 3 ? -tocopherol 3 ? -tocopherol 3 all
Tocopherol
content Vitamin C carotene Safflower 0.14 0.14 0.06 0.05 0.05 0.43 0.43 0.22 Fermented safflower seed 0.14 0.12 0.04 0.05 0.06 0.41 0.95 0.21

From Table 5 and FIG. 6, it can be seen that the vitamin C content increased from 0.43 to 0.95, and the total tocopherol content and carotene content were almost unchanged.

F. Fatty acid content comparison

Table 6 and FIG. 7 show the fatty acid contents of common safflower seeds and fermented safflower seeds.

Sample
fatty acid(%) C16: 0 C18: 0 C18: 1 C18: 2 C18: 3 Safflower 5.00 1.96 10.03 82.85 0.16 Fermented safflower seed 6.30 2.50 16.23 74.83 0.14

From Table 6 and FIG. 7, it can be seen that the fatty acid content has changed. The content of unsaturated fatty acids, oleic acid (C18: 1) and linolenic acid (C18: 0), decreased with the addition of palmitic acid (C16: 0) and stearic acid (C18: 3) decreased. In addition, it can be seen that linolenic acid (C18: 2) is decreased in the unsaturated fatty acid.

The sensory evaluation was carried out by varying the temperature of the first milking step of the first step (S100) and the second milking step of the second step (S200) during the production of the fermented safflower seed.

The results of the sensory evaluation of the safflower beverage prepared after the first milking of the washed safflower seeds at 140, 160, 180, 200 ° C and the second milking at a temperature of 50-60 ° C are shown in Table 7.

Primary milking temperature /
2nd milking temperature

Sensory characteristics
160/45 (占 폚)
160/55 (占 폚)
180/65 (占 폚) smell Flavor odor
A fragrant smell
Burnt smell
Rancidity
Odor symbol 1.9
3.2
2.5
1.6
2.9 2.0
3.6
1.8
1.5
2.9 2.4
1.3
2.0
1.0
2.3 flavor Flavored flavor
A sweet taste
Ambitious
bitter
Flavor preference 1.6
2.8
3.0
3.1
2.2 1.6
3.0
1.5
2.1
3.0 2.0
2.3
1.7
2.4
2.9

It can be seen from Table 7 that the first milking at 160 ° C and then the second milking at 55 ° C yielded the highest score in odor preference and taste preference.

In particular, when milk was first milked at 160 ℃ and second milking at 55 ℃, the smell was the best, and the flavor of Fulvirine was low and the flavor was low.

Therefore, it can be seen that it is most effective to produce safflower beverage by first milking at 160 ° C and secondly milking at 55 ° C of the present invention.

S100. The first step of roasted safflower seeds at 160 ° C for 10 minutes to first milk
S200. The second step of milking the first-step safflower seeds by boiling them in distilled water,
S300. The third step of saponification of the second milk is performed at 30 to 35 DEG C for 48 hours to naturally ferment the microorganism to be fermented
S400. Fermenting the microorganism by adding a fermenting microorganism to the naturally fermented safflower seed,
Wherein the fermenting microorganism is any one selected from the group consisting of Bacillus sp., Bacillus macerans, Bacillus circulas, and Bacillus coagulans.
S500. The fifth step of drying the microorganism-fermented safflower seeds at 40 to 45 DEG C for 5 to 10 hours
S600. A sixth step of pulverizing the dried safflower seed to 20 to 50 mesh,
S700. Mixing the 100 parts by weight of purified water with 1 part by weight of the pulped safflower seed, mixing the same at a low temperature of 40 to 60 DEG C for 10 to 20 minutes, filtering the safflower seed extract,
S701. Step 7-1 of mixing the sugar and the acidifier into the safflower seed extract, cooling to 5 to 8 DEG C, and then filling the carbonic acid gas at a pressure of 0.3 to 1.0 kg / cm < 2 &
S800. The step S8 of sterilizing the safflower seed extract and the safflower seed carbonated drink at 70 to 75 DEG C for 5 to 10 minutes

Claims

Washing the safflower seeds which have not been removed with the skin, and then roasting them at 160 ° C for 10 minutes to make a first milking step;
A second step of mixing 10 parts by weight of distilled water with 1 part by weight of the first milking safflower and milking the mixture at 50 to 60 캜;
The third step of naturally fermenting the second milking safflower seed so that the microorganism is fermented at 30 to 35 DEG C for 48 hours, while maintaining a relative humidity of 60%;
The fermented microorganism is added to the natural fermented safflower seed,
Wherein the fermenting microorganism is fermented by microorganism fermentation with any one selected from Bacillus sp., Bacillus macerans, Bacillus circulans, and Bacillus coagulans;
A fifth step of drying the microorganism-fermented safflower seed at 40 to 45 DEG C for 5 to 10 hours;
A sixth step of pulverizing the dried safflower seed into 20 to 50 mesh;
A seventh step of mixing 100 to 150 parts by weight of purified water with 1 part by weight of the pulped safflower seed, extracting it at a low temperature of 40 to 60 DEG C for 10 to 20 minutes, and then filtering to obtain an extract of safflower seed; And
And sterilizing the safflower seed extract at 70 to 75 DEG C for 5 to 10 minutes. The method of manufacturing safflower beverage using the fermented safflower seed powder

delete

The method according to claim 1,
Wherein the drying is carried out so that the water content is 5 to 20 w / w%. [5] The method for manufacturing a safflower beverage using the fermented safflower seed powder

The method according to claim 1,
In the seventh step,
(7-1) of preparing a safflower seed carbonated drink by filling carbonic acid gas after preparing an extract of safflower seed,
In the step 7-1, the sugar and the acidifier are mixed and then cooled to 5 to 8 DEG C, and then the mixture is produced at a pressure of 0.3 to 1.0 kg /
Characterized in that 7 to 15 parts by weight of the sugar and 0.01 to 0.1 part by weight of the acidulant are mixed with 100 parts by weight of the safflower seed extract.

delete