KR101657898B1 - An airborne microbe ditecting apparatus - Google Patents

An airborne microbe ditecting apparatus Download PDF

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Publication number
KR101657898B1
KR101657898B1 KR1020150075863A KR20150075863A KR101657898B1 KR 101657898 B1 KR101657898 B1 KR 101657898B1 KR 1020150075863 A KR1020150075863 A KR 1020150075863A KR 20150075863 A KR20150075863 A KR 20150075863A KR 101657898 B1 KR101657898 B1 KR 101657898B1
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South Korea
Prior art keywords
pump
collecting
sensing
buffer solution
mixed
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KR1020150075863A
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Korean (ko)
Inventor
진준형
박민준
이도훈
김준협
이동진
장호찬
전태진
이병양
Original Assignee
고려대학교 산학협력단
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

According to the present invention, a floating microbe detecting apparatus includes: a collecting unit for collecting a floating microbe floating in the air to accommodate a microbe-mixed solution obtained by mixing the collected microbe into a buffer solution accommodated therein; a sensing unit for having a sensing member prepared therein with a plurality of channels which are attached thereto with a plurality of specific antibodies to supply the microbe-mixed solution to the plurality of specific antibodies and for detecting the microbe existing in the microbe-mixed solution, upon an antigen-antibody reaction between the microbe-mixed solution and the plurality of specific antibodies; and a pump unit including a first pump for supplying the buffer solution to the collecting unit, a plurality of mixed solution supplying pumps respectively connected to the collecting unit and the plurality of channels to individually supply the microbe-mixed solution to the plurality of channels, and a second pump connected to the collecting unit and a discharging line for discharging the microbe-mixed solution or the buffer solution to the outside. Accordingly, the microbe floating in the air is detected in real time by automating a series of procedures such as collecting, cultivating, and detecting.

Description

{AN AIRBORNE MICROBE DITECTING APPARATUS}

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a floating microbe detection device, and more particularly, to a floating microbe detection device capable of detecting microbes floating in the air in real time by automatically collecting and detecting a floating microbes in the air, will be.

In bio-aerosol detection equipment floating in conventional air, microbial particles collected by passing air through a filter are cultured in a Patri dish for a certain period of time, and the number of the colonies is counted to determine the concentration of microbial particles. This method has a problem in that the time required for collecting the microbial particles and the labor cost when the manpower is input are large.

In addition, in the case of the method of collecting the microorganisms by the filter, severe dehydration phenomenon occurs due to the filtering, the survival rate of the collected microorganisms is low, and the method of collecting the microorganisms by such filtering is mainly used in a region where the degree of pollution is relatively high , It is unsuitable to collect microorganisms in general air.

Korean Patent No. 10-0868460 discloses a collection plate for an airborne microorganism collection device.

An object of the present invention is to provide a floating microbe detection device capable of detecting a microorganism floating in the air in real time by collecting airborne microorganisms in the air, and automating a series of processes of culturing and detecting.

The apparatus for detecting a suspended microorganism according to an embodiment of the present invention includes a trapping unit for trapping microorganisms floating in the air and containing a microorganism mixture in which the microorganisms trapped in the buffer solution contained therein are mixed; There is provided a sensing member provided with a plurality of channels to which a plurality of specific antibodies are attached and which provide a mixture of microorganisms with a plurality of specific antibodies, and a sensing member for detecting microorganisms present in the microorganism mixture when the microorganism mixture and the antigen- part; A first pump for supplying the buffer solution to the collecting part, a collecting part and a plurality of mixed liquid supplying pumps respectively connected to the plurality of channels for individually supplying the microbial mixed solution to the plurality of channels, and a collecting part connected to the collecting part and the discharging line of the sensing part And a second pump for discharging the microbial mixture solution or the buffer solution to the outside. The pump unit is configured such that the first pump, the plurality of mixed solution supply pumps and the second pump sequentially circulate, The microbial mixture liquid in the collecting section is supplied to the sensing section during the operation of the plurality of mixed liquid feed pumps and the microbial mixture liquid passing through the sensing section during the operation of the second pump is discharged to the outside along the discharge line, And the second pump, the first pump, and the plurality of mixed solution supply pumps are sequentially circulated, so that the operation of the second pump The buffer solution in the collecting part or the sensing part is discharged to the outside and the buffer solution newly supplied to the collecting part in the operation of the first pump is supplied and the buffer solution in the collecting part is supplied to the sensing part in operation of the plurality of mixed solution supplying pumps, It is preferable that the buffer solution in the sensing part is discharged to the outside along the discharge line during the operation of the second pump so that the collecting part and the sensing part are cleaned by the buffer solution.

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In an embodiment of the present invention, the collecting portion includes a collecting member having a closed space and provided with a receiving space therein; A collecting pump connected to the collecting member for providing vacuum pressure to the collecting member; And a collecting tube which is connected to the collecting member and through which the air flows. In operation of the first pump, the buffer solution flows into the receiving space. At the time of operating the collecting pump, To the accommodation space and mixed with the buffer solution to form a microbial mixture.

In one embodiment of the present invention, the sensing unit includes a thermoelectric element positioned to contact a mixed liquid flow pipe connecting the sensing member and the pump unit; A heat dissipating member attached to the thermoelectric element to dissipate heat generated from the thermoelectric element to the outside; And a sensing member connected to the sensing member for sensing the microorganism, wherein the sensing member is provided with a temperature-controlled microorganism mixture while passing through the thermoelectric element.

In one embodiment of the present invention, the plurality of channels include a first channel attached with a first specific antibody reactive with the first specific antigen and connected to the first mixed liquid feed pump of the pump section by the first mixed liquid flow tube; And a second channel attached with a second specific antibody that reacts with the second specific antigen and connected to the second mixed liquid feed pump of the pump section by the second mixed liquid flow tube.

In one embodiment of the present invention, it is preferable that a temperature control unit is connected to the thermoelectric element, and the temperature controller controls the operating temperature of the thermoelectric element to control the temperature of the microorganism mixture flowing in the mixed liquid flow tube.

In one embodiment of the present invention, the sensing member is preferably a carbon nanotube field effect transistor (CNT-FET).

The present invention enables automatic detection of a series of processes for collecting and detecting suspended microorganisms in the air in a liquid phase, thereby enabling detection time and manpower input to be reduced compared to the conventional method.

Further, according to the present invention, the operator can automatically clean the collecting part and the sensing part by operating the pump part without cleaning the collecting part and the sensing part, thereby reducing the working time and manpower usage, Can be increased.

The present invention is designed so that the sensing member can be driven at a low temperature, so that the service life of the sensing member can be extended.

In addition, the present invention can detect microorganisms reacting with specific antibodies by attaching different specific antibodies to one sensing member.

FIG. 1 schematically shows a configuration of an apparatus for detecting a suspended microorganism according to an embodiment of the present invention. Referring to FIG.
2 schematically shows a connection state diagram between a collecting part and a sensing part in an embodiment of the present invention.
FIG. 3 (a) schematically shows a plan view of a sensing member having two channels, FIG. 3 (b) schematically shows a configuration of a sensing unit and a flow diagram of a microbial mixture flow introduced into two channels, respectively ,
FIG. 4 is a graph showing a change in current value with time in the temperature change of ammonia gas.
FIG. 5 is a graph showing a change in current value with time and a change in current value after cleaning of the sensing part when providing a pure buffer solution and a microorganism mixture.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, an apparatus for detecting a suspended microorganism according to a preferred embodiment of the present invention will be described with reference to the accompanying drawings.

The apparatus for detecting a suspended microorganism according to an embodiment of the present invention is for detecting a microorganism by performing a series of processes for collecting and detecting microorganisms floating in air.

1, the apparatus 100 for detecting a suspended microorganism includes a collecting unit 110, a sensing unit 150, and a pump unit 130. As shown in FIG.

As shown in FIGS. 1 and 2, the collecting unit 110 collects microorganisms floating in the air and includes a collecting member 111, a collecting cover member 111a, a collecting pump 113, 115).

The collecting member 111 is provided with a receiving space 112 therein. Here, the accommodation space 112 is a space in which a buffer solution or a mixture of microorganisms is accommodated. The 1a flow tube 131a, the 2a flow tube 132a, the 1a mixture liquid flow tube 133a, and the 2a mixture liquid flow tube 134a are connected to the lower part of the collecting member 111. The 1a flow tube 131a, the 2a flow tube 132a, the 1a mixture liquid flow tube 133a and the 2a mixture liquid flow tube 134a may be installed on the collecting member 111 so as to communicate with the receiving space 112.

The buffer solution flows into the accommodation space 112 through the first flow tube 131a when the first pump 131 is operated. Here, the buffer solution is the solution introduced through the first flow tube 131a. The buffer solution is also referred to as a "buffer solution ", and is intended to keep the hydrogen ion concentration constant without being greatly affected when a certain amount of acid or base is applied from the outside.

The air existing outside the collecting member 111 flows into the collecting cover member 111a through the collecting tube 115 and then flows into the receiving space 112 of the collecting member 111 . In this process, the microorganisms floating in the air are mixed with the buffer solution. In this embodiment, the buffer solution in a state where microorganisms are mixed is referred to as a " microorganism mixture solution ".

A collecting cover member (111a) is provided on the collecting member (111). The collecting cover member 111a has a structure for sealing the collecting member 111. [ A collecting pump 113 and a collecting tube 115 are connected to the collecting cover member 111a. The collection pump 113 may be a vacuum pump.

The collecting tube 115 is a passage through which air existing outside the collecting member 111 flows. The air introduced into the collecting cover member 111a through the collecting tube 115 during the operation of the collecting pump 113 is supplied to the receiving space 112 of the collecting member 111 by the vacuum pressure, ≪ / RTI >

Hereinafter, the pump unit 130 will be described.

The pump unit 130 is disposed between the collecting unit 110 and the sensing unit 150 so as to supply the microbial mixed solution from the collecting unit 110 to the sensing unit 150. The pump unit 130 includes a first pump 131, a second pump 132, and a mixed solution supply pump.

The first pump 131 is connected between the collecting part 110 and the first storage tank 137. Specifically, the first pump 131 is connected to the collecting unit 110 by the first flow tube 131a and is connected to the first storage tank 137 by the first flow tube 131b. Here, the first storage tank 137 is a member in which the buffer solution is accommodated. The buffer solution in the first storage tank 137 flows into the first pump 131 along the first b flow pipe 131b and is discharged and accommodated along the first flow pipe 131a And is supplied to the space 112.

The second pump 132 is connected between the collecting part 110 and the second storage tank 138. Specifically, the second pump 132 is connected to the collecting portion 110 by the second flow tube 132a, and is connected to the second storage tank 138 by the second flow tube. Here, the second storage tank 138 is a space in which the cleaning solution of the accommodation space 112 or the microorganism mixed solution sensed by the sensing unit 150 is accommodated. That is, the second storage tank 138 is a member that receives the waste solution.

The second pump 132 is operated separately from the first pump 131. In this embodiment, the second pump 132 is operated when cleaning the accommodation space 112 under the control of the pump control unit 136. [ In operation of the second pump 132, the buffer solution or microbial mixture in the first storage tank 137 flows into the second pump 132 along the 2a flow tube 132a and is discharged to the 2b flow tube, The second storage tank 138 is connected to the discharge line 135 of the sensing unit 150 along the 2b flow tube.

Here, the discharge line 135 of the sensing unit 150 is connected between the sensing unit 150 and the second storage tank 138. The discharge line 135 of the sensing unit 150 is connected to the microbiological mixture liquid that has passed through the sensing unit 150 and the buffer solution that cleans the accommodation space 112 of the collecting member 111 during operation of the second pump 132, .

The mixed liquid feed pumps 133 and 134 are connected between the collecting unit 110 and the sensing unit 150 by a mixed liquid flow pipe to supply the mixed microbial liquid in the collecting unit 110 to the sensing unit 150. The mixed liquid supply pump is connected to the collecting unit 110 separately from the first pump 131 and the second pump 132.

In an embodiment of the present invention, a plurality of mixed liquid supplying pumps may be provided. In order to simplify the description, the first mixed liquid supplying pump 133 and the second mixed liquid supplying pump 134 The number of channels may be varied according to the number of channels provided in the sensing member 154, which will be described later. Also, since the mixed liquid feed pump is divided into the 'first mixed liquid feed pump 133 and the second mixed liquid feed pump 134', the mixed liquid flow tubes connected to the first mixed liquid flow pipes 133a and 133b, (134a, 134b) '.

The first mixed liquid supply pump 133 and the second mixed liquid supply pump 134 individually connect the collecting unit 110 and the sensing unit 150 with each other.

Specifically, the first mixed liquid supply pump 133 is connected between the collecting unit 110 and the first channel 154c of the sensing unit 150 by the first mixed liquid flow pipes 133a and 133b. Here, the first mixed solution flow tubes 133a and 133b are referred to as a first mixed solution flow tube 133a and a first mixed solution flow tube 133b, depending on the connected positions.

The first mixed liquid supply pump 133 is connected to the collecting member 111 by the first a mixed liquid flow pipe 133a and is connected to the first channel 154c by the first b mixed liquid flow pipe 133b. The first mixed liquid supply pump 133 is connected to the pump control unit 136. The pump control unit 136 controls whether the first mixed liquid supply pump 133 is operated. The pump control unit 136 may control the first mixed liquid feed pump 133 so that the first mixed liquid feed pump 133 supplies the microbial mixed liquid to the first channel 154c at a flow rate of 1 mm / The pump control unit 136 can individually control the operation of the first pump 131, the second pump 132, the first mixed liquid supply pump 133, and the second mixed liquid supply pump 134. [

The second mixed liquid feed pump 134 is connected between the collecting unit 110 and the second channel 154d of the sensing unit 150 by a second mixed liquid flow pipe. Here, the second mixed solution flow tube is divided into a second mixed solution flow pipe 134a and a second mixed solution flow pipe 134b depending on the connected position.

The second mixed solution supply pump 134 is connected to the collecting member 111 by the second mixed solution flow pipe 134a and is connected to the second channel 154d by the second mixed solution flow pipe 134b. The second mixed liquid supply pump 134 is connected to the pump control unit 136. The pump control unit 136 may control the second mixed solution supply pump 134 so that the second mixed solution supply pump 134 supplies the microbial mixed solution to the second channel 154d at a flow rate of 1 mm /

Hereinafter, the sensing unit 150 will be described.

As shown in FIGS. 2 and 3, the sensing unit 150 receives the microbial mixture in the collecting unit 110 and detects microorganisms present in the microbial mixture. The sensing unit 150 includes a sensing case 151, a sensing holder 151a, a heat radiating member 152, a thermoelectric element 153, a sensing member 154, a temperature regulating unit 158 and a measuring member 159 do.

The sensing case 151 serves to protect the heat radiating member 152, the thermoelectric element 153, and the sensing member 154. The heat dissipating member 152 is disposed in contact with the thermoelectric element 153 inside the sensing case 151. The heat dissipating member 152 is a member for dissipating the heat of the thermoelectric element 153 to the outside when the thermoelectric element 153 operates.

As shown in Fig. 2, the thermoelectric element 153 is connected to the surface of the heat radiating member 152 so as to be in contact with it. That is, the thermoelectric element 153 is provided with a heat dissipating member 152 on one side and heat transferable by the first b mixed liquid flow tube 133b and the second b mixed liquid flow tube 134b on the other side. The thermoelectric element 153 is for cooling the microbial mixture flowing along the first b mixed solution flow tube 133b and the second mixed solution flow tube 134b to a predetermined temperature.

The thermoelectric element 153 is connected to the temperature regulator 158 so that the temperature of the microbial mixture can be controlled by the temperature regulator 158. The thermoelectric element 153 can adjust the operating temperature of the sensing member 154 from 5 ° C to room temperature by the temperature controller 158, thereby extending the service life of the sensing member 154.

The thermoelectric element 153 is fixed by the sensing holder 151a. Here, the sensing holder 151a is for fixing the sensing member 154, the thermoelectric element 153, the first b mixed liquid flow tube 133b and the second b mixed liquid flow tube 134b to be in a predetermined position.

The sensing holder 151a is preferably made of a material having a high thermal conductivity such as aluminum. The sensing holder 151a can transmit the cool air provided from the thermoelectric element 153 to the first b mixed liquid flow pipe 133b and the second b mixed liquid flow pipe 134b.

The first b mixed solution flow pipe 133b and the second b mixed solution flow pipe 134b via the thermoelectric element 153 are connected to the sensing member 154. [ Specifically, the end of the first b mixed liquid flow pipe 133b is connected to the first channel 154c of the sensing member 154 and the end of the second b mixed liquid flow pipe 134b is connected to the second channel 154d of the sensing member 154 .

The sensing member 154 is for detecting microorganisms present in the microbial mixture. In this embodiment, the sensing member 154 is preferably a carbon nanotube field effect transistor (CNT-FET).

2 and 3 (a), the sensing member 154 is formed by patterning chromium or gold on the Si / SiO 2 substrate 154a. Here, the printed pattern 154b having a gold or chromium material is formed by embossing on the Si / SiO 2 substrate 154a. The carbon nanotubes adhere to the grooves formed as the printed pattern 154b is embossed on the Si / SiO2 substrate 154a. A measurement member 159 is connected to the print pattern 154b.

The print pattern 154b is provided with a first channel 154c and a second channel 154d. In the present embodiment, for convenience of description, a portion connected to the first channel 154c is referred to as a 'first carbon nanotube 155a' and a portion connected to the second channel 154d is referred to as a ' Quot; nanotube 155b ". However, this is for convenience of explanation, and the first carbon nanotubes 155a and the second carbon nanotubes 155b do not mean separate members.

The sensing member 154 according to the present embodiment may be configured such that the first specific antigen 157a is specifically bound to the first specific antibody 156a immobilized on the surface of the first carbon nanotube 155a, And transmits an electrical signal to the measuring member 159 when the second specific antigen 157b is specifically bound to the second specific antibody 156b immobilized on the surface of the tube 155b.

The first specific antibody 156a is attached to the surface of the first carbon nanotube 155a. The first specific antibody 156a is an antibody that reacts with the first specific antigen 157a. IgG or IgM may be used as the first specific antibody 156a, but the present invention is not limited thereto.

The microbial mixture discharged from the first b-mixed flow tube 133b passes through the first channel 154c and is supplied to the first carbon nanotubes 155a. At this time, if there is a first specific antigen 157a reacting with the first specific antibody 156a among the microorganisms contained in the microbial mixture, an antigenic antibody reaction occurs and an electrical signal is generated in the printed pattern 154b, (159).

In this embodiment, the second specific antibody 156b is attached to the surface of the second carbon nanotube 155b. The second specific antibody 156b is an antibody that reacts with the second specific antigen 157b. The second specific antibody 156b may be an antibody that distinguishes it from the first specific antibody 156a. As the second specific antibody 156b, IgG or IgM may be used, but is not limited thereto.

The microbial mixed solution discharged from the second b-mixed flow tube 134b passes through the second channel 154d and is supplied to the second carbon nanotubes 155b. At this time, if there is the second specific antigen 157b reacting with the second specific antibody 156b among the microorganisms contained in the microbial mixture solution, the antigen antibody reaction occurs and an electrical signal is generated in the printing pattern 154b, (159).

In this embodiment, the measurement member 159 is electrically connected to the print pattern 154b, and can detect the microorganism from the change in the current value of the print pattern 154b upon the antigen-antibody reaction of the specific antigen with the specific antibody .

Hereinafter, with reference to FIG. 4, a sensitivity test of the sensing member 154 according to the type of gas and temperature will be described. FIG. 4 is a graph showing a change in current value with time at a temperature change of water at room temperature and ammonia gas.

As shown in FIG. 4, it can be seen that the change of the current value I is almost insignificant even if the water (H 2 O) at room temperature (25 ° C) elapses. On the other hand, ammonia gas in the case of (NH 4 OH), can be seen that the current value is started at a current value measured, 80nA of ammonia gas (NH 4 OH) at 25 ℃ reduced to approximately 70nA Thirty minutes later, 35 It can be seen that the current value started at 70 nA when the current value of ammonia gas (NH 4 OH) is measured at 30 ° C is lowered to about 60 nA after 30 minutes. However, when the current value of ammonia gas (NH 4 OH) is measured at 7 ° C, it is understood that the current value is drastically reduced to about 30 nA at the elapse of 30 minutes at a current value of 80 nA or more.

The graph of FIG. 4 shows that the ammonia gas (NH 4 OH) has a variation width of the current value of about 10 nA at room temperature (25 ° C.) or above at room temperature (35 ° C.) The change in the current value with the passage of time is greater than about 50 nA. This is because a gas such as an ammonia gas (NH 4 OH) has a lower sensitivity because the gas solubility is lowered at a higher temperature. In general, gas solubility is inversely proportional to temperature, and temperature is inversely proportional to sensitivity.

4, it can be seen that the present invention can improve the sensing efficiency of the sensing member 154 by cooling the sensing member 154 and the microbial mixture using the thermoelectric element 153.

Hereinafter, with reference to FIG. 5, a description will be given of a current value change over time and a change in current value after the cleaning of the sensing unit 150 in providing the pure buffer solution and the microorganism mixture. The experimental graph shown in FIG. 5 is a graph of change in current value sensed at the sensing member 154 at 7 ° C.

As shown in FIG. 5, it can be seen that the sensing member 154 can not detect microorganisms even when the pure buffer solution containing no microorganisms passes through the sensor, and measures the same current value of 10 nA. On the graph, the first current value change line (1) is a current value change of the buffer solution with time.

 However, in the case of the microbial mixture containing microorganisms, the sensing member 154 can measure the change of the current value over a period of 0 to 5 minutes. In the case of the microbial mixed solution, the change of the current value with time is dependent on the second current value change line 2 and the third current value change line 3. This is because the first specific antibody 156a attached to the sensing member 154 and the specific antigen reacting with the second specific antibody 156b are different from each other. Here, the second current value change line (2) is a current value change line that reacts when the Aspergillus niger strain is contained in the microbial mixture, and the third current value change line (3) A current value change line. Here, the Alternaria alternata strain and the Aspergillus niger strain are inducers of black fungi.

After the elapse of 5 minutes, if the cleaning process of the sensing unit 150 is performed, the current value change sensed by the sensing unit 150 becomes closer to the current value of the buffer solution as time elapses, 150 are washed.

The cleaning process of the sensing unit 150 is as follows.

First, the second pump 132 is operated to discharge the mixed microbial fluid present in the receiving space 112 of the collecting unit 110 to the second storage tank 138. After the operation of the second pump 132 is stopped, the first pump 131 is operated to introduce a clean buffer solution into the accommodation space 112. Thereafter, a clean buffer solution, that is, a buffer solution not containing microorganisms is supplied to the sensing unit 150 while the first mixed solution supply pump 133 and the second mixed solution supply pump 134 are operated, The sensing unit 150 is cleaned. Then, the buffer solution that has been cleaned by the sensing unit 150 is supplied to the second storage tank 138 through the discharge line 135. This process is repeated for a few minutes, and the sensing unit 150 is cleaned.

Hereinafter, the cleaning process of the collecting unit 110 will be described.

The apparatus 100 for detecting a suspended microorganism according to the present embodiment is configured to detect the presence of the trapping portion 110 in the trapping portion 110 by using the first pump 131 and the second pump 132, Lt; / RTI >

The second pump 132 is operated to discharge the mixed microbial fluid present in the receiving space 112 of the collecting unit 110 to the second storage tank 138. Thereafter, the first pump 131 is operated to supply the buffer solution to the receiving space 112, so that the clean buffer solution can be used to wash the receiving space 112. The pump controller 136 may control the first pump 131 to supply the buffer solution to the accommodation space 112 at a flow rate of 10 mm /

Alternatively, instead of the buffer solution, the washing liquid may be supplied into the receiving space 112 by using the first pump 131. [ When the receiving space 112 of the collecting part 110 is cleaned by the washing solution or the clean buffer solution, the washing solution existing in the receiving space 112 or the buffer solution after washing is activated by the second pump 132, 132a to the second pump 132 and then to the discharge line 135 in the second flow tube to discharge it to the second storage tank 138. [

According to the present invention, the operator can automatically clean the collecting unit 110 and the sensing unit 150 without cleaning the collecting unit 110 and the sensing unit 150, The use of manpower can be reduced, thereby increasing the efficiency of microbial detection work.

In addition, the present invention can increase the sensing efficiency by adjusting the temperature of the sensing member 154 and maintaining the temperature of the sensing member 154 at a temperature at which microorganisms can be effectively detected.

In addition, the present invention can detect microorganisms reacting with specific antibodies by attaching different specific antibodies to one sensing member 154. In addition, the present invention can detect microorganisms by varying the type of a specific antibody that reacts with the specific type of antigen.

100: Flotation microorganism detection device 110:
111: collecting member 111a: collecting cover member
112: accommodation space 113: collection pump
115: collection tube 130: pump part
131: first pump 131a: first flow tube
131b: first b flow pipe 132: second pump
132a: 2a flow tube 132b: 2b flow tube
133: First mixed liquid supply pump 133a: First mixed liquid flow tube
133b: first b mixed liquid flow tube 134: second mixed liquid supply pump
134a: the 2a mixed liquid flow tube 134b: the 2b mixed liquid flow tube
137: first storage tank 138: second storage tank
150: sensing unit 151: sensing case
151a: sensing holder 152: heat dissipating member
153: thermoelectric element 158: temperature regulator
159: measuring member 154: sensing member

Claims (8)

A trapping unit for trapping microorganisms floating in the air and containing a microorganism mixture in which the trapped microorganisms are mixed in a buffer solution contained therein;
There is provided a sensing member having a plurality of specific antibodies attached thereto and provided with a plurality of channels for providing the microbial mixture with the plurality of specific antibodies, wherein the microbial mixture solution and the plurality of specific antibodies, A sensing unit for sensing the microorganism; And
A first pump for supplying the buffer solution to the collecting part; a plurality of mixture liquid supply pumps connected to the collecting part and the plurality of channels, respectively, for separately supplying the microorganism mixture to the plurality of channels; And a pump unit connected to a discharge line of the sensing unit and having a second pump for discharging the microbial mixture solution or the buffer solution to the outside,
The pump unit includes:
Wherein the buffer solution is supplied to the collecting part during operation of the first pump while the first pump, the plurality of mixed solution supplying pumps and the second pump are sequentially circulated and the buffer solution is supplied during operation of the plurality of mixed solution supplying pumps Wherein the microorganism mixed liquid in the collecting unit is provided to the sensing unit and the microbial mixture liquid passing through the sensing unit during operation of the second pump is discharged to the outside along the discharge line,
The microbial mixture liquid in the collecting part or the sensing part is discharged to the outside during the operation of the second pump while the second pump, the first pump and the plurality of mixed solution supplying pumps are sequentially circulated, Wherein the buffer solution newly supplied to the collecting portion is supplied during operation of the pump, the buffer solution in the collecting portion is supplied to the sensing portion when the plurality of mixed solution supplying pumps are operated, Wherein the buffer solution is actuated to be washed by the buffer solution while the buffer solution is discharged to the outside along the discharge line to wash the collecting part and the sensing part.
delete delete The apparatus according to claim 1,
A collecting member having a closed space and provided with a receiving space therein;
A collecting pump connected to the collecting member and providing vacuum pressure to the collecting member; And
And a collection tube connected to the collection member and into which the air flows,
Wherein when the first pump is operated, the buffer solution flows into the accommodation space,
Wherein the air present on the outside of the collecting member during operation of the collecting pump is supplied to the receiving space through the collecting tube and mixed with the buffer solution to form the microorganism mixed solution.
The apparatus of claim 1, wherein the sensing unit
A thermoelectric element placed in contact with the mixed liquid flow pipe connecting the sensing member and the pump section;
A heat dissipating member attached to the thermoelectric element to dissipate heat generated from the thermoelectric element to the outside; And
And a measuring member connected to the sensing member for detecting the microorganism,
Wherein the sensing member is provided with the microbial mixture liquid whose temperature is controlled while passing through the thermoelectric element.
6. The method of claim 5,
A first channel attached with a first specific antibody reacting with the first specific antigen and connected to the first mixed liquid feed pump of the pump section by a first mixed liquid flow tube; And
A second specific antibody which reacts with the second specific antigen is attached and is connected to the second mixed liquid feed pump of the pump section by the second mixed liquid flow tube.
6. The method of claim 5,
A thermostat is connected to the thermoelectric element,
Wherein the temperature regulator controls the operating temperature of the thermoelectric element to regulate the temperature of the microbial mixture flowing in the mixed-solution flow tube.
6. The method of claim 5,
Wherein the sensing member is a carbon nanotube field effect transistor (CNT-FET).
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KR20080082858A (en) * 2007-03-09 2008-09-12 한국화학연구원 Microorganism detection sensor using carbon nanotube transister array and method of detecting microorganism using thereof
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