JP2005140666A5 - - Google Patents

Claims (13)

A reaction vessel formed on the substrate;
Formed on the substrate, and at least a reagent and a sample can be independently supplied to the reaction vessel part, and a flow path part ,
It is arranged in the reaction vessel, can capture on the surface at least one of the substance in the reagent and the substance in the specimen, and the substance in the reagent and the substance in the specimen are combined. A cantilever that is the stage where the reaction takes place ;
A microchip for inspection. Furthermore,
A waste liquid tank formed on the substrate and communicating with the reaction vessel;
The inspection microchip according to claim 1, further comprising: an openable / closable valve portion that is provided in the flow path portion and controls fluid flow. The flow path portion can be supplied to the reactor section the reagent and the specimen and the washing liquid independently, testing microchip of claim 1 or 2. The inspection microchip according to any one of claims 1 to 3, wherein a plurality of the reaction tank parts are provided, and a plurality of the cantilevers independent from each other are arranged in each of the reaction tank parts . The inspection microchip according to any one of claims 1 to 4 ,
And sensing means can detect deflection of the cantilever,
Inspection device having In addition,
A pump capable of making the inside of the reaction vessel negative pressure;
Valve control means capable of arbitrarily opening and closing the valve portion;
The inspection apparatus according to claim 5 , comprising: The inspection device according to claim 5 or 6 , wherein the detection means optically detects bending of the cantilever. The inspection device according to claim 5 or 6 , wherein the detection means detects the bending of the cantilever as a change in electric resistance. Supplying a reagent from a flow path formed in the substrate to a reaction tank formed on the substrate, and attaching a substance in the reagent on a cantilever provided in the reaction tank;
Supplying a cleaning liquid from the flow path to the reaction tank, leaving only the substance adhering to the cantilever, and discharging the other reagents from the reaction tank;
Supplying a specimen from the flow path section to the reaction tank section and capturing the substance in the specimen on the cantilever by reacting with the substance adhering to the cantilever;
Measuring the amount of deflection of the cantilever while the substance in the specimen is captured on the cantilever;
Identifying and / or quantifying a substance in the specimen based on the amount of deflection of the cantilever ;
Including inspection methods. The test method according to claim 9 , wherein one of the substance in the reagent and the substance in the specimen is an antigen, and the other is an antibody. Supply of the reagent, the specimen, and the cleaning liquid from the flow path section to the reaction tank section, a pump capable of setting the inside of the reaction tank section to a negative pressure, and fluid flow provided in the flow path section independently controlled by actuating the openable valve unit for controlling the inspection method according to claim 9 or 10. The test method according to claim 11 , wherein a reaction time between the substance in the reagent and the substance in the sample is arbitrarily set by controlling the opening and closing timing of the valve unit. Wherein a plurality of reaction bath section, may be provided in each of a plurality of said cantilevers each of said reaction vessel portion independent of each other, performing different test independently the each cantilever, any one of the claims 9-12 Inspection method described in 1.