KR101645936B1 - Dental caries preventive and halitosis deodorant comprising chestnut inner shell as an active ingredient and preparation method thereof - Google Patents

Abstract

The present invention relates to an extracting step of extracting a Chestnut inner shell with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof; And filtering the extract mixture obtained in the extracting step to obtain a filtrate from which impurities have been removed. The present invention also relates to a method for producing a deodorant composition for removing odor elimination containing the extract as an active ingredient.

Description

[0001] The present invention relates to a dental caries preventive or oral deodorant composition and a method for manufacturing the dental caries preventive and oral deodorant composition,

The present invention relates to a deodorant composition for removing a bad breath and a method for producing the same, which comprises an effective amount of a udder extract having a deodorizing power against a volatile sulfur compound as a cause of bad breath and a property of inhibiting proliferation of the causative bacteria.

The cause of bad breath is largely divided into oral causes and external causes. Oral causes include oral microorganisms or untreated dental caries (tooth decay) and periodontal disease, There are various causes such as respiratory-related diseases, gastrointestinal diseases, and fasting, menstruation, smoking, and drinking.

Among these causes, bad breath is mainly caused by volatile sulfur compounds generated by decomposition of food waste precipitated between microorganisms by the microorganisms. These volatile sulfur compounds include hydrogen sulfide, methyl mercaptan (CH ₃ SH) Dimethyl sulfide (S (CH ₃ ) ₂ ), and the like. Among these, methyl mercaptan is known as a major component of halitosis [Kleinberg and Westbay, 1990; Loesche and Kazor, 2002].

Specifically, the volatile sulfur compounds are known to occur through the fermentation of gram negative anaerobic bacteria against peptide and protein substrates containing sulfur in the mouth, and it is known that Porphyromonas gingivalis causes periodontal disease In particular, the occurrence of halitosis by sulfur compounds is increased. Other bad breath inducing substances include volatile amine compounds such as trimethylamine, aldehydes, fatty acids, and ammonia.

In the mouth, Streptococcus mutans mutans), Streptococcus sanggwi varnish (Streptococcus sanguinis , and Streptococcus salivarius . Among them, Streptococcus mutans is known to be a major causative organism (Loeshe 1986; Bowen 2002; Beighton 2005). In general, tooth decay is a disease caused by the interaction of pathogenic bacteria, residual food, and saliva in oral cavity. Dental cavities are infectious diseases caused by oral bacteria. Especially, dextran produced by Streptococcus mutans produces a plaque on the surface of the teeth. The plaque is formed by the bacteria forming the plaque and the sugar (sucrose), starch And the generated acid damages the enamel surface (enamel) of the tooth (demineralization), thereby advancing the cavity and generating bad breath together.

Therefore, in the past, it has been proposed to use antibiotics or a compound such as fluorine, which inhibits the proliferation of Streptococcus mutans, in an oral product such as toothpaste to prevent plaque formation and to suppress the occurrence of tooth decay, Various antibiotics and bactericides have been widely used as oral compositions. However, these antibiotics and fungicides have disadvantages that they can be used only as a therapeutic agent because it is difficult to use for a long period of time because it may cause adverse effects on the body and the appearance of resistant bacteria in oral cavity.

Korean Patent Laid-Open Publication No. 2005-0010082 discloses a method of preparing a silver toothpaste added with a silver solution, a silver powder or a silver foil film so as to have effects such as tooth whitening, tooth decay prevention and periodontal disease prevention. Korean Patent No. 0691792 discloses a method of preparing a dentine-containing cavity containing a lignan derivative excellent in antibacterial activity against Streptococcus mutans bacteria which are caries-inducing bacteria and Porphyromonas japonica which is a periodontal disease-causing bacteria, Patent Publication No. 2001-0001476 discloses a method for preventing and treating periodontal disease, which comprises oral germicide, periodontal disease inhibitor, and flavonoid to remove pathogenic bacteria, periodontal disease, In the composition of the present invention.

However, when antibiotics are continuously used, microorganisms may become resistant, cause vomiting, and the like, and the growth of microorganisms in the oral cavity may be inhibited. Therefore, it is urgently required to separate anticaries, anti-periodic diseases and bad breath removing substances from natural products without side effects.

Chestnut (Castanea crenata Sieb) is a deciduous broad-leaved tree belonging to the oak tree, and its fruit (Castanea crenata) is composed of fruit, endothelium and mantle. The endothelium, also called ufpy, has a pungent taste, so it is generally used after being used for edible use, and the removed endothelium is being abandoned as waste. Korean Patent Laid-Open No. 10-2006-0110579 discloses a skin whitening composition containing quercetin as an active ingredient extracted from uri, and is characterized by inhibiting c-kit activity involved in melanogenesis and differentiation and maturation of melanocytes In addition, it has been reported that the tubercle bark extract can exhibit antioxidant and anti-inflammatory activity and thus can be utilized as a functional material for atopic disease.

The first problem to be solved by the present invention is to provide a deodorant composition for removing bad breath including an extract of Quercus as an active ingredient.

A second object of the present invention is to provide a method for manufacturing the deodorant composition for removing the bad breath.

In order to achieve the above object, the present invention provides a deodorant composition for removing bad breath, which comprises an extract of Chestnut inner shell as an active ingredient.

According to an embodiment of the present invention, the deodorant composition for removing the bad breath may further include at least one selected from green tea extract, raspberry extract, black tea extract and acorn bark extract.

According to an embodiment of the present invention, the tuberculosis extract may be contained in an amount of 0.001 to 50% by weight based on the total weight of the deodorant composition for deodorization.

According to an embodiment of the present invention, the tuberculosis extract may be an extract extracted with any one solvent selected from water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

According to one embodiment of the present invention, the bad breath removal can be accomplished through removal of volatile sulfur compounds in the oral cavity or inhibition of bacterial growth caused by tooth decay.

On the other hand, the deodorant composition for removing bad breath, which comprises the Chestnut inner shell extract as an active ingredient, may be prepared by dissolving the Chestnut inner shell in any one solvent selected from water, lower alcohols having 1 to 4 carbon atoms, An extracting step of extracting; And filtering the extracted mixture obtained in the extracting step to obtain a filtrate from which impurities have been removed.

According to an embodiment of the present invention, the extraction step may further include 0.1 to 10 parts by weight of the base with respect to 100 parts by weight of the extraction solvent.

According to an embodiment of the present invention, the extraction step may further include irradiating ultrasonic waves, and the ultrasonic irradiation may be performed at 15 to 25 kHz and 500 to 800 watts for 2 to 60 minutes .

The deodorant composition for removing bad breath containing the tubular extract of the present invention as an active ingredient can effectively inhibit bad breath by effectively adsorbing or chemically binding a substance such as a sulfur compound inducing halitosis to the oral cavity, It is possible to prevent or inhibit infectious oral diseases that cause bad breath such as tooth decay and periodontal disease.

FIG. 1 is a graph showing the content of condensed tannin in the tuberous extract according to an embodiment of the present invention.
FIG. 2 is a graph showing the total flavonoid content of the tuba extract according to an embodiment of the present invention. FIG.
FIG. 3 is a graph showing the deodorizing power against methyl mercaptan according to the concentration of tubercle extract at 60 ° C. extraction temperature according to an embodiment of the present invention.
FIG. 4 is a graph showing the deodorizing power against methyl mercaptan according to the concentration of tubercle extract at 90 ° C. extraction temperature according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The deodorant composition for removing bad breath of the present invention contains Chestnut inner shell extract as an active ingredient.

According to the present invention, the component particularly effective for removing bad breath among the components of the tubular extract may be a flavonoid or a tannin compound. The tannin may be a hydrolyzable tannin such as gallotannins and ellagitannins hydrolyable tannins, and condensed tannins such as catechin, epicatechin and epilocatechin.

According to the present invention, it is possible to suppress the spread of odor by suppressing vaporization by adsorbing a causative substance causing bad breath, and in particular, a condensed tannin component such as epicatechin, And exhibits a high antioxidative effect due to a free radical inhibiting effect, so that it is possible to inhibit the growth of harmful bacteria in the oral cavity and thus to suppress halitosis.

According to the present invention, it is preferable that the tuberculosis extract is crosslinked with the causative substance causing bad breath, thereby reducing the odor perception or deodorizing the bad breath by forming new molecules that exhibit unpleasant odor or neutralizing the odor. For example, flavonoids in the uri extracts can inhibit halitosis through condensation, polymerization or addition reaction with -NH 2, -SH, which is a functional group of ammonia or methyl mercaptan, which causes odor through the hydroxyl groups possessed in the molecular structure And tannin can form an esterification reaction with -NH 2 and -SH to suppress halitosis.

According to the present invention, the content of the tubercle bark extract in the deodorant composition for deodorizing the bad breath may vary somewhat depending on the formulation, but may be 0.001 to 50% by weight. If the content of the tuberculosis extract is less than 0.001% by weight, it is difficult to expect a malodor removing effect. If the content of the tuberculosis extract is more than 50% by weight, stabilization of the formulation is not preferable.

According to the present invention, the deodorant composition for deodorizing the bad breath may further comprise at least one selected from green tea extract, raspberry extract, black tea extract and acorn bark extract, in addition to the uri extract as an effective ingredient.

Green tea extract, black tea extract and raspberry extract have high tannin content such as catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, and gallic acid, and also contain a large amount of polyphenol and flavonoid components. It is preferable because it can effectively work.

In addition, the acorn bark extract is preferable because it contains polyphenols, especially polyphenols and flavonoids including tannin components such as gallic acid, triglyceride, and gallotannin, effectively acting to remove bad breath.

According to the present invention, when at least one selected from the green tea extract, the raspberry extract, the black tea extract and the acorn bark extract is further added, the extracts may be contained in a proportion of 0.01 to 10 parts by weight based on 10 parts by weight of the udder extract have.

According to the present invention, when the additive is one selected from green tea extract, raspberry extract, black tea extract and acorn bark extract, the content of the additive may be 0.01 to 10 parts by weight based on 10 parts by weight of the extract,

In the case of two or more kinds of additives, the content of the mixed additives may be 0.01 to 10 parts by weight based on 10 parts by weight of the extract.

The ratio of the green tea extract, raspberry extract, black tea extract and acorn bark extract of additives is not particularly limited. For example, 0.05 to 2 parts by weight of raspberry extract, 0.05 to 2 parts by weight of black tea extract, 0.05 to 2 parts by weight of the extract.

The tuberous extract according to the present invention may be an extract obtained by extracting tuberose with any one solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, preferably 30 to 80% To 4% by weight of an aqueous solution of a lower alcohol is more preferable because it has a high extraction yield and a high content of components effective for removing bad breath in the extract.

In the present invention, the lower alcohol having 1 to 4 carbon atoms may be selected from methanol, ethanol, propanol and butanol, preferably ethanol.

According to the present invention, the removal of the bad breath may be the removal of odor-inducing substances and volatile sulfur compounds in the oral cavity or the inhibition of bacterial growth caused by tooth decay.

On the other hand, the deodorant composition for removing the bad breath containing the tuberculosis extract as an active ingredient may be prepared by extracting the Chestnut inner shell with any one solvent selected from water, lower alcohols having 1 to 4 carbon atoms or a mixed solvent thereof ; And filtering the extracted mixture obtained in the extracting step to obtain a filtrate from which impurities have been removed.

According to the present invention, the udder is a chestnut of the chestnut, preferably a chestnut powder which is obtained by crushing the chestnut of the chestnut. When the chestnut powder is used in the extraction, the surface area is widened, It is easy to extract and the yield can be improved.

According to the present invention, the extraction can be carried out by adding 5 to 50 parts by weight per 100 parts by weight of the solvent, and can be carried out by stirring at a temperature ranging from 25 to 100 DEG C for 0.5 to 12 hours. If the agitation temperature is lower than the above range, the extraction yield of substances effective for removing bad breath is lowered, but it is not economical because equipment for lowering the temperature is required. If the agitation temperature exceeds the above range, the effective ingredient effective for removing bad breath is destroyed , Substances which are not effective for removing bad breath can be extracted together to obtain an extract having reduced deodorizing power, which is not preferable.

According to the present invention, the stirring temperature is in a temperature range of 50 to 95 ° C., because the food cell tissue is softened and the component effective for removing bad breath is easily eluted, so that an extract having excellent deodorizing power can be obtained.

According to the present invention, the solvent may be any one selected from the group consisting of water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably an alcohol aqueous solution of 30 to 80% by volume, 30 to 70% by volume aqueous ethanol solution.

According to the present invention, the extraction may be performed by further containing 0.1 to 10 parts by weight of a base relative to 100 parts by weight of the extraction solvent. The base may be NaOH or KOH. According to the present invention, when the content of the base is less than the above range, the degree of increase in the extraction yield of the tuberculosis extract is insignificant, and when the above range is exceeded, the extract effective to hydrolyze a substance effective for removing bad breath, It is not preferable. More preferably 0.5 to 3 parts by weight of a base per 100 parts by weight of the solvent to obtain an extract having a high content of hydrolyzable tannins, condensed tannins, total flavonoids and total phenols, The content of condensed tannin such as kylocatechin and the content of total flavonoid are improved, so that an extract having improved deodorizing power against bad breath can be produced.

According to the present invention, the step of irradiating the ultrasonic wave during the stirring may further include the step of increasing the extraction yield of the useful component effective for removing the bad breath.

The ultrasonic wave irradiation may be performed at 15 to 25 kHz and 500 to 800 watts for 2 to 60 minutes. If the irradiation time is less than the above range, the ultrasonic wave irradiation time is too short and the effect by ultrasonic irradiation is insignificant. Exceeding this range is undesirable since the components of the substance effective for removing bad breath may be destroyed or may be transformed into a structure that is not effective for bad breath removal.

The deodorant composition for removing bad breath according to the present invention is not limited to the formulations and may be formulated using a commonly available formulation method such as, for example, solution, gaggle solution, spray, toothpaste and the like.

Hereinafter, the present invention will be described in more detail with reference to preferred embodiments. It will be apparent, however, to those skilled in the art that these embodiments are for further explanation of the present invention and that the scope of the present invention is not limited thereby.

Example

Manufacturing example  One

The chestnut skin was washed, peeled, dried naturally for 12 hours, and pulverized with a grinder to obtain a jellyfish powder.

Manufacturing example  2

30 g of green tea powder was added to 100 g of water and stirred at 60 캜 to prepare green tea extract.

Manufacturing example  3

30 g of raspberry powder was added to 100 g of water and stirred at 60 캜 to prepare a raspberry extract.

Manufacturing example  4

30 g of black tea powder was added to 100 g of water and stirred at 60 캜 to prepare black tea extract.

Manufacturing example  5

30 g of acorn shell powder was added to 100 g of water and stirred at 60 캜 to prepare an acorn bark extract.

Example 1 Preparation of deodorant composition for removing bad breath

Example  1.1

To 10 g of water was added 1 g of the jellyfish powder of Preparation Example 1, and the mixture was stirred at 25 DEG C for 0.5 hour to prepare a jellyfish extract. The jellyfish extract was filtered to remove the jellyfish powder and impurities, and the filtrate was concentrated under reduced pressure to prepare a jellyfish extract.

Example 1.2

The uri extract was prepared according to the method of Example 1.1 except that it was stirred at 60 캜 instead of 25 캜.

Example  1.3

The uri extract was prepared according to the method of Example 1.1 except that it was stirred at 90 캜 instead of 25 캜.

Example  2

The extracts were prepared according to the methods of Examples 1.1 to 1.3, except that 30%, 50% and 70% aqueous ethanol solutions were used instead of water as the extraction solvent, respectively.

Example  3

The uri extract was prepared according to the methods of Examples 1.1 to 1.3, except that 30%, 50% and 70% aqueous methanol solutions were used instead of water as the extraction solvent, respectively.

Example  4

The uri extract was prepared according to the methods of Examples 1.1 to 1.3 except that 1% (w / v) aqueous NaOH solution was used instead of water as the extraction solvent, respectively.

Example  5

The uri extract was prepared in the same manner as in Example 1.2 except that 2% (w / v) NaOH aqueous solution was used instead of water as the extraction solvent, respectively.

Test Example  1. Component analysis

end. Condensed tannin content

Condensed tannin content was measured according to the vanillin-HCl method (Price et al., 1978). The solids obtained by lyophilization of the udder extracts were re - dispersed in 10 ml of Absolute methanol to prepare a uvi extract with a concentration of 5000 ppm. 2 ml of 0.5% vanillin reagent and 2 ml of 4% HCl were added to 1 ml of tubercle bark extract and allowed to react at room temperature for 20 minutes. After the reaction was completed, the absorbance was measured at 500 nm using a UV-Vis spectrometer. The standard test was carried out in the same manner as in the case of using the methanol extract instead of the tubular extract and the standard curve was obtained by setting the concentration of (+) - catechin hydrate (Sigma Co., USA) to 10-50 ㎍ / Tannin content (mg catechin equivalent / g ufpy) was obtained.

B. Total phenol content

The total phenol content of the tuberous extract was measured by Folin & Ciocalteu method. Concentration is added and the Folin-Ciocalteu reagent 800㎕ to 100 ppm of yulpi 200㎕ extract, were added 7.5% Na ₂ CO ₃ solution 2㎖ after 10 minutes. After incubation for 90 minutes in the dark, the absorbance was measured at 765 nm using a UV-Vis spectrometer. Total polyphenol content was expressed as mg Gallic acid equivalents (GAE) / g Chestnut inner shell.

C. Total flavonoid content

The total flavonoid content of the jujube extracts was measured by the aluminum chloride colormetric method (Zhishen J et al. 1999). The lyophilized extracts were lyophilized and resuspended in 10 ㎖ of Absolute methanol. 75 ㎕ of 5% NaNO ₂ and 1 ㎖ of distilled water were added to 0.5 ml of tuberculosis extract and allowed to react for 5 minutes. Then, 10% AlCl ₃ 150 μl of the reagent was added, and the mixture was further reacted at room temperature for 6 minutes. Then, 500 μl of 1M NaOH and 275 μl of distilled water were added to the final 2.5 ml, followed by reaction at room temperature for 15 minutes. After completion of the reaction, the absorbance was measured at 510 nm using a UV-Vis spectrometer. The standard curve was obtained by adjusting the concentration of (+) - catechin hydrate (Sigma Co., USA) to 10-50 ㎍ / ㎖, and the flavonoid content (mg catechin equivalent / g ufpy).

Test Example  1.1 Analysis of components by extraction solvent

(30%, 50% and 70%), aqueous ethanol solution (30%, 50% and 70%), methanol and ethanol were used as solvents to obtain condensed tannin content The mixture was stirred for 30 minutes at < RTI ID = 0.0 > 0 C < / RTI > for 30 minutes to prepare a tubercle extract. The condensed tannin content was calculated and shown in Table 1 below.

division
Condensed tannin content
(mg catechin / g urine) Flavonoid content
(mg catechin / g urine)  Example 1.2 water 95.63 255.07 Example 2.1
ethanol
30% 118.21 264.81 Example 2.2 50% 193.47 481.61 Example 2.3 70% 172.15 451.15 Example 3.1
Methanol 30% 115.28 260.45 Example 3.2 50% 192.97 479.82 Example 3.3 70% 170.45 448.21

As shown in Table 1, both condensed tannins and flavonoids were extracted with the highest content in an aqueous 50% alcohol solution, and when the concentration of alcohol was increased by 50% or more, the extractable content tended to decrease.

Test Example  1.2. Analysis of components by extraction temperature

(Example 1), 50% ethanol (Example 2.2), 50% methanol (Example 3.2), and 1 (ethyl alcohol) were used as solvents to confirm condensed tannins, total phenol and total flavonoid contents depending on extraction temperature and solvent To determine the content of condensed tannin according to the extraction temperature, the extracts were incubated at 25 ° C. for 24 hours in the presence of 5% (w / v) NaOH (Example 4) and 2% (w / v) NaOH (Example 5) The mixture was stirred at 60 캜 and 90 캜 for 30 minutes to prepare a tubercle extract. This is shown in Table 2 below (unit: mg catechin / g ufpy).

division Example 1 Example 2.2 Example 3.2 Example 4 Example 5
Condensed tannin 25 ℃ 85.13 171.30 172.47 240.80 238.63 60 ° C 95.63 193.47 192.97 241.97 244.47 90 ° C 100.97 229.97 226.30 244.63 228.13
Total phenol
25 ℃ 221.37 242.74 211.11 - - 60 ° C 287.18 521.79 464.53 470.94 473.93 90 ° C 411.11 558.12 510.26 463.67 457.69 Total flavonoid
25 ℃ 237.07 446.94 438.57 699.25 708.82 60 ° C 255.61 481.61 479.82 765.02 775.78 90 ° C 292.08 593.42 602.39 831.39 764.42

As shown in Table 2, the total phenols, condensed tannins, and total flavonoids were increased by extraction with a base in a solvent at the time of extraction, and it was found that the higher the extraction temperature, the more soluble components were extracted Respectively.

Test Example  2. Deodorizing force  Measure

In order to confirm the effect of removing the bad breath, the deodorization test for methyl mercaptan, which is the main cause of bad breath, was performed using the Manual SPME-GC method. 1 ml of a watery extract having a concentration of 500,000 ppm was mixed with methyl mercaptan (50 ppm) and sealed. Then, a sealed vial containing the uri extract and methyl mercaptan was reacted at 37 ° C for 10 minutes to remove volatile substances from the head space of the vial Respectively. Then, SPME fiber; Methylmercaptan captured in head space was adsorbed for 10 minutes using 100 μm PDMS (Supelco), and methylmercaptan-adsorbed SPME fiber was directly injected into the GC injector (250 ° C.) And analyzed with desorption. As a control, deodorizing power was measured by using water instead of uri extract.

division Methyl mercaptan Control group Example
One Example 2.2 Example 3.2 Example
4 Example
5 60 ° C
peak area 223325.7 218591.2 163858.0 150930.6 141164.6 124529.0 111114.8 Deodorizing force (%) - 2.12 26.6 32.42 36.79 44.26 50.25 90 ° C
peak area 223325.7 218434.9 156637.3 125967.3 136403.1 106990.9 119254.7 Deodorizing force (%) - 2.19 29.86 43.59 38.92 52.09 46.6

As shown in Table 3, as the extraction temperature was increased, the deodorizing power against methyl mercaptan was improved, which was similar to the increase of total phenol, condensed tannin and total flavonoid content. In addition, the deodorizing power of the extract was significantly improved when the base was treated. However, when the content of the base was 2% or more, the deodorizing power was lowered when the extraction temperature was high. And is destroyed at a high concentration of the base.

Test Example  3. Antibacterial experiments on oral bacteria

The present inventors have investigated the effect of the tuberculosis extract according to the present invention on the strain by using Streptococcus mutans , a causative bacterium of tooth decay. Streptococcus mutans (KCCM 11823), which is a freeze-dried cucumber, was suspended in a BHI liquid medium and incubated at 37 ° C for 24 hours to activate the bacteria, which were then cultured in a BHI solid medium for 24 hours. Experiments were carried out using paper disk assay for antimicrobial test. The extracts of Puccinia japonica Extract at 90 ℃ were treated with 50% ethanol at 0.5, 1 and 2 mg / disc, respectively. Control group was treated with 5% phenol solution and BHI liquid The experiment was carried out using the medium. After culturing under optimal culture conditions for 24 hours, the growth inhibition size (mm) was measured and the antimicrobial activity thereof was verified based on the results.

division Urine extract (1 ml) BHI badge 5% phenol Treatment concentration 0.5 mg / disc 1 mg / disc 2 mg / disc 1 mg / disc 1 mg / disc Growth restriction (mm) 5 8 9 - 11

As shown in Table 4, it was confirmed that the growth inhibition volume of Streptococcus mutans was also increased as the concentration of the tubercle extract was increased. It is presumed that the tannin component contained in the tuber extract is reacted with the protein of the cell wall of the microorganism and the enzyme activity of the microorganism is inhibited to exhibit the antibacterial effect.

Test Example  4. Antioxidant effect

The DPPH radical scavenging activity was investigated in order to investigate the antioxidative effect of the extracts on the extractive solvent and extraction temperature. (1% NaOH) and Example 5 (2% NaOH), which had a concentration of 1000 ppm, of Example 2, Example 2.2 (50% ethanol), Example 3.2 500 μl of DPPH (1,1-diphenyl-2picrylhydrazyl) reagent (6 × 10 ^-5 mol / L in ethanol) was added and reacted in the dark for 30 minutes. Absorbance was measured at 517 nm using UV-Vis spectrometer. Antioxidant activity was expressed in mM Trolox equivalent / g chestnut inner shell and is shown in Table 5 below.

division Example 1 Example 2.2 Example 3.2 Example 4 Example 5
Antioxidant power 25 ℃ 159.79 170.20 169.75 - - 60 ° C 162.60 172.56 170.66 168.68 169.44 90 ° C 164.96 174.61 173.47 169.67 167.62

As shown in Table 5, the antioxidant activity was found to be the highest in the tubular extracts extracted with 90% dpt in 50% ethanol aqueous solution, and the antioxidative activity was also decreased with the extraction temperature of 2% NaOH aqueous solution Which is similar to the deodorization test.

Test Example  5. Ultrasonic treatment Deodorizing force  Test

The effects of ultrasonic treatment and ultrasonic irradiation time on extraction were measured. Ultrasonic waves were generated and processed at 25 ° C, 20 kHz, 750 watt and 20 amplitude using an ultrasonic wave extractor (SEEC-SONIC II, UL-Tech, Korea). In order to keep the temperature constant, Respectively. Ultrasonic waves were irradiated for 0, 10, 30, 45, 60, 90, and 120 minutes, respectively, and the tubular extracts were prepared according to the method of Example 1.2 to perform a deodorization test.

Methyl mercaptan Example 1.2 Example 6.1 Investigation time - 0 10 30 45 60 90 120 peak area 223325 163858 163250 162134 156997 159901 163920 165037 Deodorizing force (%) 0 26.6 26.9 27.4 29.7 28.4 26.6 26.1

Claims (9)

The present invention relates to a deodorant composition for removing volatile sulfur compounds in oral cavity containing, as an active ingredient, an extract of Chestnut inner shell extracted with a solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof. The method according to claim 1,
Wherein the composition further comprises at least one member selected from the group consisting of hot water extract of green tea at 60 ° C, hot water extract at 60 ° C for raspberry, hot water extract at 60 ° C for tea, and hot water extract at 60 ° C for acorn skin. . The method according to claim 1,
The composition for removing odorous sulfur compounds in oral cavity according to claim 1, wherein the tubular extract is contained in an amount of 0.001 to 50% by weight based on the total weight of the composition. delete delete An extraction step of extracting a Chestnut inner shell with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof; And
And filtering the extracted mixture obtained in the extracting step to obtain a filtrate from which impurities have been removed. The method for preparing a deodorant composition for removing volatile sulfur compounds in oral cavity according to claim 1, The method according to claim 6,
Wherein the extracting step is performed by adding 0.1 to 10 parts by weight of NaOH to 100 parts by weight of the extraction solvent. The method of claim 6, wherein
Wherein the extracting step further comprises the step of irradiating ultrasound waves. ≪ RTI ID = 0.0 > 11. < / RTI > The method of claim 8, wherein
Wherein the ultrasonic irradiation is performed at 15 to 25 kHz and 500 to 800 watts for 2 to 60 minutes.

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